Apparatus for producing figures on a carrier

Abstract

An apparatus for creating graphics on a carrier is provided. The device of the present invention includes a bottom 1, a peripheral wall 2, a first baffle 31 having a first protrusion 311, a second baffle 32 having a second protrusion 321, and a slot 4. The peripheral wall is formed on the upper surface of the bottom. The first baffle and the second baffle are respectively located on opposite sides of the peripheral wall and parallel to the bottom. The slot is surrounded and formed by the bottom, the peripheral wall, the first baffle, and the second baffle.

Description

[Technical field]

[0001] The present invention relates to an apparatus, in particular to an apparatus for creating graphics on a carrier. [Background technology]

[0002] Immunofluorescence (IF) is a widely used technique in biotechnology, mainly used to stain biological samples such as cells and tissue slices to detect and localize certain proteins in the biological samples. In traditional staining methods, users manually manipulate with a marker pen to create a closed geometric shape in plan view around the biological sample on a carrier (e.g., a glass slide), creating a water-tight barrier around the sample and thus allowing the staining reagent to remain on the biological sample.

[0003] In addition, conventional techniques for automatically detecting biological samples generally use image recognition devices to capture images of the samples for subsequent observation, and such automated image recognition devices have the ability to capture images appropriately according to the shape of the object to be measured.

[0004] However, the above-mentioned conventional staining methods cannot produce a repeatable, regular and bounded figure. In other words, when detecting a biological sample in a closed figure with an automated image recognition device, it is difficult to accurately capture and recognize the desired image of the sample based on an irregular closed figure. Therefore, there is a need in the field for an auxiliary device to solve the above problem. Summary of the Invention

[0005] In order to solve the above problems, the present invention provides an apparatus for creating a pattern on a carrier, which includes: a bottom, a peripheral wall, a first baffle, a second baffle, and a slot. The peripheral wall is formed on the upper surface of the bottom. The first baffle and the second baffle are respectively located on opposite sides of the peripheral wall and parallel to the bottom. The slot is surrounded and formed by the bottom, the peripheral wall, the first baffle, and the second baffle. The first baffle has a first protrusion, the first protrusion extending from the first baffle toward the second baffle. The second baffle has a second protrusion, the second protrusion extending from the second baffle toward the first baffle. [Brief description of the drawings]

[0006] [Figure 1A-1B] 1 is a schematic diagram of an apparatus for creating graphics on a carrier in accordance with at least one embodiment of the present invention; [Diagram 2] 1 is a schematic diagram of an embodiment for creating a graphic on a carrier and a created graphic according to at least one embodiment of the present invention; [Diagram 3] 1 is a schematic diagram of a pattern created according to at least one comparative example of the present invention; DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0007] The following examples are provided to describe the present invention in detail. Those skilled in the art can easily understand the advantages and effects of the present invention by reading the contents disclosed in this specification, and can also implement or apply the present invention in other different embodiments. Therefore, the following examples for implementing the present invention can be modified and / or changed without going against the scope of different aspects and applications of the present invention. In addition, any element or step included in the scope of the present invention can be combined with any other element or step disclosed in any embodiment of the present invention.

[0008] Unless otherwise stated, the articles "a", "one", "the", and "said" are used herein to refer to the grammatical object "one or more than one" (i.e., at least one). Also, unless otherwise stated, "or", " / " and "and / or" are interchangeable.

[0009] The phrase "at least one" as used herein refers to one or more parts and should be understood as meaning at least one part selected from any one or more of the listed parts, but not necessarily including at least one of all the listed parts and not excluding any combination of elements from the listed elements. This definition implies that parts other than the parts listed in the parts list to which the phrase "at least one" refers may be present as needed, whether or not related to the listed parts. Thus, as a non-limiting example, "at least one of A and B" (or equivalently "at least one of A or B" or equivalently "at least one of A and / or B") can refer to at least one A in one embodiment and can refer to more than one A and no B as needed (and can include parts other than B as needed). In another embodiment, it can refer to at least one B and can refer to more than one B and no A as needed (and can include parts other than A as needed). In another embodiment, the term may refer to at least one A, and may optionally refer to more than one A, or may refer to at least one B, and may optionally refer to more than one B (and may include other components as appropriate).

[0010] As used herein, the terms "comprise," "include," "contain," "have," and other variations are intended to cover non-exclusive inclusions. For example, when an object is described as "comprising" a limiting element, unless otherwise stated, it can further include other components, parts, members, structures, regions, portions, devices, systems, steps, or connections, and does not exclude other limiting elements.

[0011] The numerical ranges used herein are inclusive and combinable, and any numerical value included in the numerical ranges herein can be considered as a maximum or minimum value, from which subranges can be derived. For example, the numerical range "75mm-90mm" includes any subrange between the minimum value of 75mm and the maximum value of 90mm, for example, subranges such as 75mm-85mm, 76mm-88mm, 80mm-90mm, etc. Also, if necessary, multiple numerical values ​​used herein can be selected as maximum and minimum values ​​to derive numerical ranges. For example, the numerical ranges 75mm-83mm, 83mm-90mm, and 75mm-90mm are derived from the numerical values ​​75mm, 83mm, and 90mm.

[0012] The term "about" in the present invention means within a typical tolerance range in the art. For example, "about" can be understood to mean a difference from the mean value of about 2 times the standard deviation or less. When "about" is placed before a series of numbers or ranges, it should be understood that "about" modifies each of the series of numbers or ranges. For example, the numerical values ​​are intended to cover a variation of ±20%, ±10%, ±5%, ±1%, ±0.5% or ±0.1% of the numerical value. The numerical ranges used herein are inclusive and combinable, and any numerical value included in the numerical ranges herein can be considered as a maximum or minimum value, from which subranges can be derived. For example, the numerical range "20-30%" should be understood to include any subrange between the minimum value of 20% and the maximum value of 30%, such as the subranges of 20-25%, 25-30%, and 22.5-27.5%. Such variation in numerical values ​​may be caused by measurement errors or similar considerations. Alternatively, the term "about" means within the average standard deviation that is acceptable to one of ordinary skill in the art. Unless otherwise expressly stated, all numerical ranges, amounts, values, and percentages disclosed herein should be understood to be modified in all circumstances by the term "about."

[0013] Spatial terms, such as "top", "bottom", "left", "right", "front", "back" and similar terms, may be used herein to describe the relationship of one component / feature to another component / feature(s) as shown in the figures. It should be understood that the spatial terms are intended to cover different orientations of the components / features, other than the orientation shown in the figures. For example, if the components / features in the figures are inverted, a component described as "below other components / features" or "under other components / features" would be defined as "above other components / features". Thus, the exemplary term "top" can include both the above and below orientations. Similarly, unless otherwise specified, the terms "top", "bottom", "left", "right", "front", "back" and similar terms are merely terms of description herein.

[0014] The ordinal numbers in the present invention, such as "first", "second" and "third", are not intended to indicate the order of one component / feature relative to another component / feature or the order in a manufacturing method, but are only used to distinguish components / features having the same name by modifying the components / features.

[0015] Unless otherwise specified herein, there is no particular limitation on the manufacturing method of the device for creating a pattern on a carrier, and the related description is merely intended to illustrate a preferred structure of said device. In some embodiments, the device for creating a pattern on a carrier can be manufactured using additive manufacturing or subtractive manufacturing methods, but the invention is not limited thereto.

[0016] In at least one embodiment of the present invention, the first baffle and the second baffle may be rectangular.

[0017] In at least one embodiment of the present invention, the first protrusion and the second protrusion may have a shape comprised of a semicircle and a rectangle.

[0018] In at least one embodiment of the present invention, the diameter of the semicircle corresponds to the length of the rectangle, and the diameter of the semicircle may be about 6 mm or more, for example, but not limited to, about 6 mm to 10 mm, about 6.2 mm to 9.8 mm, or about 6.4 mm to 9.6 mm. In some embodiments, the diameter of the semicircle may be about 6, 6.2, 6.4, 6.6, 6.8, 7, 7.2, 7.4, 7.6, 7.8, 8, 8.2, 8.4, 8.6, 8.8, 9, 9.2, 9.4, 9.6, 9.8, or 10 mm, preferably about 8 mm, but the present invention is not limited thereto.

[0019] In at least one embodiment of the present invention, the width of the rectangle may be about 0.5 mm or more, such as, but not limited to, about 0.5 mm to 1.5 mm, about 0.6 mm to 1.4 mm, or about 0.7 mm to 1.3 mm. In some embodiments, the width of the rectangle may be about 0.5, 0.6, 0.7, 0.8, 0.9, 1, 1.1, 1.2, 1.3, 1.4, 1.5 mm, and preferably about 1 mm, although the present invention is not limited thereto.

[0020] In at least one embodiment of the present invention, the first baffle and the first protrusion may be symmetrical with the second baffle and the second protrusion.

[0021] In at least one embodiment of the present invention, the peripheral wall may include a first side wall, a second side wall, and a third side wall, and may have a U-shape. In some embodiments, the first side wall and the second side wall may be located on opposite sides of the bottom and parallel to each other. In some embodiments, the third side wall may be perpendicular to the first side wall and the second side wall. In some embodiments, any two of the first side wall, the second side wall, and the third side wall may be connected or not connected.

[0022] In at least one embodiment of the present invention, the slot may be surrounded and defined by a bottom, a first sidewall, a second sidewall, a third sidewall, a first baffle, and a second baffle.

[0023] In at least one embodiment of the present invention, the length of the interior space of the slot may be greater than about 75 mm, such as, but not limited to, about 75 mm to 90 mm, about 76 mm to 89 mm, or about 77 mm to 88 mm. In some embodiments, the length of the interior space of the slot may be about 75, 76, 77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, or 90 mm, preferably about 79 mm, although the present invention is not limited thereto.

[0024] In at least one embodiment of the present invention, the width of the interior space of the slot may be greater than about 40 mm, such as, but not limited to, about 40 mm to 50 mm, about 41 mm to 49 mm, or about 42 mm to 48 mm. In some embodiments, the width of the interior space of the slot may be about 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, or 50 mm, preferably about 46 mm, although the present invention is not limited thereto.

[0025] In at least one embodiment of the present invention, the height of the internal space of the slot may be about 1 mm or more, such as, but not limited to, about 1 mm to 10 mm, about 2 mm to 9 mm, or about 3 mm to 8 mm. In some embodiments, the height of the internal space of the slot may be about 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 mm, preferably about 2 mm, although the present invention is not limited thereto.

[0026] In at least one embodiment of the present invention, the apparatus for creating graphics on the carrier can further include a first opening, which is formed in the peripheral wall between a location connected to the first baffle and the second baffle.

[0027] In at least one embodiment of the present invention, the length of the first opening can be greater than about 60 mm, such as, but not limited to, about 60 mm to 70 mm, about 61 mm to 69 mm, or about 62 mm to 68 mm. In some embodiments, the length of the first opening can be about 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, or 70 mm, preferably about 65 mm, although the present invention is not limited thereto.

[0028] In at least one embodiment of the present invention, the width of the first opening may be greater than about 25 mm, such as, but not limited to, about 25 mm to 30 mm, about 25.5 mm to 29.5 mm, or about 26 mm to 29 mm. In some embodiments, the width of the first opening may be about 25, 25.5, 26, 26.5, 27, 27.5, 28, 28.5, 29, 29.5, or 30 mm, preferably about 27.5 mm, although the present invention is not limited thereto.

[0029] In at least one embodiment of the present invention, the apparatus for creating graphics on the carrier can further include a second opening, which is formed in the peripheral wall between a location not connected to the first baffle and the second baffle.

[0030] In at least one embodiment of the present invention, the device for creating graphics on the carrier can further include an insertion hole, which is formed on the side not connected to the peripheral wall at the bottom.

[0031] In at least one embodiment of the present invention, the peripheral wall may further include a notch formed at two ends of the U-shape.

[0032] In at least one embodiment of the present invention, the carrier may be a glass slide, a cover slip, or a polished glass.

[0033] In at least one embodiment of the present invention, the closed figure may be a waterproof ring.

[0034] In at least one embodiment of the present invention, the peripheral walls can be used to limit the range of movement of the carrier in the slot.

[0035] In at least one embodiment of the present invention, the first opening is used to expose the top surface of the carrier from the slot, thereby allowing a user to draw a line segment on the top surface of the carrier along the edge of the first protrusion or the second protrusion.

[0036] In at least one embodiment of the present invention, the second opening is used to move the carrier within the slot.

[0037] In at least one embodiment of the present invention, the notch is used to prevent the carrier from slipping by allowing a user to clamp one end of the carrier with their fingers.

[0038] In at least one embodiment of the present invention, the device of the present invention can be sized based on the carrier as needed.

[0039] In at least one embodiment of the present invention, the first and second protrusions may be a combination of a symmetrical semicircle and a rectangle, in order to avoid the problem that the ends of the first and second line segments drawn along the first and second protrusions do not overlap and cannot form a closed figure.

[0040] The following further describes exemplary embodiments of the present invention, but is not intended to limit the scope of the present invention, and those skilled in the art can make various adjustments and modifications without departing from the spirit and scope of the present invention. EXAMPLES

[0041] In this embodiment, the apparatus and slide glass shown in Figure 1A and Figure 1B are prepared, and then, by manually operating with a marker pen, the apparatus is used as an auxiliary tool to create a closed pattern on the surface of the slide glass as a waterproof ring in the manner shown in Figure 2.

[0042] Please refer to Figures 1A and 1B, which are schematic diagrams of an apparatus for creating a pattern on a carrier in at least one embodiment of the present invention. As shown in Figure 1A, the apparatus for creating a pattern on a carrier includes a bottom 1, a peripheral wall 2, a first baffle 31, a first protrusion 311, a second baffle 32, a second protrusion 321 and a slot 4. The peripheral wall 2 is located on the upper surface of the bottom 1 and includes a first side wall 21, a second side wall 22 and a third side wall 23. The first side wall 21 is located on the left side of the bottom 1, the second side wall 22 is located on the right side of the bottom 1, and the third side wall 23 is located on the rear side of the bottom 1. The first side wall 21, the second side wall 22 and the third side wall 23 are connected to each other and form a U-shape. The first baffle 31 is located on the left side of the peripheral wall 2, the second baffle 32 is located on the right side of the peripheral wall 2, and the first baffle 31 and the second baffle 32 are parallel to the bottom 1. The first baffle 31 includes a first protrusion 311 that extends from the first baffle 31 to the second baffle 32, and the second baffle 32 includes a second protrusion 321 that extends from the second baffle 32 to the first baffle 31. The first baffle 31 and the second baffle 32 are symmetrical rectangular, and the first protrusion 311 and the second protrusion 321 are symmetrical in shape, and they are configured as a semicircle and a rectangle. The slot 4 is surrounded and formed by the bottom 1, the first side wall 21, the second side wall 22, the third side wall 23, the first baffle 31 and the second baffle 32. The device in this embodiment further includes a first opening 51, a second opening 52 and an insertion hole 53, the first opening 51 being formed between the positions connected to the first baffle 31 and the second baffle 32 in the surrounding wall 2, the second opening 52 being formed between the positions not connected to the first baffle 31 and the second baffle 32 in the surrounding wall 2, and the insertion hole 53 being formed on one side of the bottom 1 that is not connected to the surrounding wall 2. The peripheral wall 2 further includes notches 54 formed at the two ends of the U-shape. As shown in Figure 1B, the device in this embodiment may be designed to have the following sizes:The length L1 of the inner space of the slot 4 is about 75mm-90mm, the width W1 is about 40mm-50mm, and the height (not shown) is about 1mm-10mm, the length L2 of the first opening 51 is about 60mm-70mm, the width W2 is about 25mm-30mm, and the first protrusion 311 and the second protrusion 321 are composed of a semicircle and a rectangle, where the diameter D of the semicircle (corresponding to the length of the rectangle) is about 6mm-10mm, and the width W3 of the rectangle is about 0.5mm-1.5mm. The device can place a slide glass (not shown) as a carrier, which has a length of about 75mm, a width of about 26mm, and a thickness of about 1mm.

[0043] Please refer to FIG. 2, which is a schematic diagram of an embodiment of making a pattern on a carrier according to at least one embodiment of the present invention and the made pattern. As shown in FIG. 2, in step S101, the slide glass 6 is placed on one side of the slot 4 that is connected to the first baffle 31, and a marker pen is used to draw a first line segment (black dashed line) along the edge of the first protrusion 311 on the slide glass 6. And in step S102, the slide glass 6 is placed on one side of the slot 4 that is connected to the second baffle 32, and a marker pen is used to draw a second line segment (black dashed line) along the edge of the second protrusion 321 on the slide glass 6. After completing the above steps, a waterproof ring formed by the first line segment and the second line segment can be obtained, which is a closed standard circle. Comparative Example

[0044] Without using the device of the present invention, a closed pattern is drawn on the surface of the slide glass manually using a marker pen to form a waterproof ring.

[0045] Please refer to Figure 3, which is a schematic diagram of a shape created according to at least one comparative example of the present invention. As shown in Figure 3, the waterproof ring created on the glass slide 6 is a closed irregular shape (i.e., not a standard circle).

[0046] As can be seen from the above, the device of the present invention can be used to create a standard pattern, which allows users to create a repeatable, regular and bounded closed pattern around a biological sample to form a water-tight ring, thus achieving the technical effect of improving the accuracy of the closed pattern and at the same time improving the operation efficiency of an automated image recognition device.

[0047] The above-described embodiments are merely preferred embodiments of the present invention, and any equivalent modifications and variations made within the scope of the claims of the present invention are intended to be included in the scope covered by the present invention. [Explanation of symbols]

[0048] 1: Bottom 2: Surrounding walls 21:First side wall 22:Second side wall 23: Third side wall 31: First baffle 311: First protrusion 32: Second baffle 321:Second protrusion 4: Slots 51:First opening 52:Second opening 53: Insertion port 54: Notch 6: Slide glass L1, L2: Length W1, W2, W3: Width D: Diameter S101, S102: Step

Claims

1. bottom, a peripheral wall located on an upper surface of the base; a first baffle and a second baffle, respectively, located on opposite sides of the peripheral wall and parallel to the bottom; a slot defined by the bottom, the peripheral wall, the first baffle and the second baffle; and the first baffle has a first protrusion extending from the first baffle toward the second baffle, and the second baffle has a second protrusion extending from the second baffle toward the first baffle. A device for producing shapes on a carrier.

2. 2. The apparatus of claim 1, wherein the first baffle and the second baffle are rectangular, and the first protrusion and the second protrusion have a shape comprised of a semicircle and a rectangle.

3. The apparatus of claim 1 , wherein the first baffle and the first projection are symmetrical with the second baffle and the second projection.

4. The peripheral wall includes a first side wall, a second side wall, and a third side wall and has a U-shape; the first side wall and the second side wall are located on opposite sides of the bottom and are parallel to each other; the third side wall is perpendicular to the first side wall and the second side wall; Any two of the first side wall, the second side wall, and the third side wall are connected or not connected to each other; 2. The apparatus of claim 1, wherein the slot is surrounded and defined by the bottom, the first sidewall, the second sidewall, the third sidewall, the first baffle, and the second baffle.

5. The apparatus of claim 1 , further comprising a first opening formed in the peripheral wall between a location connected to the first baffle and the second baffle.

6. The apparatus of claim 1 , further comprising a second opening formed in the peripheral wall between a location of the first baffle and the second baffle that is not connected to the peripheral wall.

7. The device of claim 1 further comprising an insertion opening formed on a side of said bottom that is not connected to said peripheral wall.

8. 5. The apparatus of claim 4, wherein said peripheral wall further includes notches formed at two ends of said U-shape.

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