Improved LAMP construct

Abstract

The present invention provides improved LAMP constructs for enhanced processing, including specific fragments of the LAMP luminal domain for delivering antigens to immune cells. These LAMP constructs can be used to treat diseases, particularly allergies, infectious diseases, diabetes, hyperproliferative disorders, and / or cancer. When administered to a subject, the improved LAMP constructs enable the presentation of appropriately structured three-dimensional epitopes for the generation of an immune response. The improved LAMP constructs can be multivalent molecules and / or can be provided as part of a multivalent vaccine containing two or more LAMP constructs. The improved LAMP constructs described herein can also be used to generate antibodies when administered to non-human vertebrates. [Selection diagram] None

Description

[Technical field]

[0001] The present invention relates to improved LAMP constructs and their use in treating subjects suffering from infectious diseases, diabetes, allergies, hyperproliferative disorders and / or cancer.Furthermore, the improved LAMP constructs described herein can be used to generate antibodies in non-human vertebrates, preferably the genome of the non-human vertebrate comprises at least partially human immunoglobulin regions and / or humanized immunoglobulin regions.Also described are prime-boost protocols utilizing the improved LAMP constructs described herein. [Background technology]

[0002] In the following discussion, certain articles and methods are described for background and introduction purposes. Nothing contained herein should be construed as an "admission" of prior art. Applicants expressly reserve the right, if necessary, under applicable legal provisions to demonstrate that the articles and methods referenced herein do not constitute prior art.

[0003] DNA vaccines are new promising candidates for developing both preventive and therapeutic vaccines. They have been proven safe, and the lack of immune response to the vector backbone could be a crucial advantage if repeated cycles of vaccination are required to achieve clinical benefit. However, one of the drawbacks of conventional DNA vaccines is their poor immunogenicity in humans. A key limiting step in the immunogenicity of epitope-based DNA vaccines could be the access of epitopes to the pathway of MHCII presentation to T cells, which seems to be a stochastic manner in the case of vaccines without targeted technology.

[0004] US Patent No. 5,633,234 describes a chimeric protein containing a modified influenza hemagglutinin (HA) antigenic domain and a cytoplasmic endosomal / lysosomal targeting signal that effectively targets the antigen to that compartment. The antigenic domain was processed and peptides derived therefrom were presented on the cell surface with the cooperation of major histocompatibility complex (MHC) class II molecules. The cytoplasmic tail of LAMP-1 was used to form the endosomal / lysosomal targeting domain of the chimeric protein.

[0005] No. 8,318,173 extended these initial observations by describing chimeric proteins (and the corresponding DNA encoding these proteins) that comprised the HIV-1 Gag protein inserted between the complete luminal and transmembrane domains of LAMP-1. This construct was subsequently reported to be introduced into dendritic cells and to target the MHC II pathway.

[0006] This approach has proven useful in increasing cellular and humoral responses to several viral antigens, including human papillomavirus E7, dengue virus membrane protein, HIV-1 gp160 membrane protein, HIV-1 p55 Gag, West Nile membrane protein, hepatitis C virus NS3 protein, and cytomegalovirus pp65 (see, e.g., J.Immunol.166:5250-5257,2001). The enhanced immune response is due to the colocalization of LAMP and MHC II, as well as more efficient processing and delivery of antigenic peptides. Furthermore, it has been reported that LAMP targeting results in the presentation of an increased number of immunogenic epitopes, thus inducing a qualitatively expanded immune response compared to non-targeted antigens. For example, Fernandes et al.,2000,Eur.J.Immunol.30(8):2333-43, showed an increase in the number of presentation peptides of LAMP-delivered OVA antigen encoded in a vaccinia vector. Of the 12 peptides generated from exogenously supplied OVA, 9 were presented by the OVA / LAMP chimera, compared with only 2 presented by the LAMP-free construct.

[0007] It has been determined that the cytoplasmic domain of LAMPs (together with the signal sequence and transmembrane domain) is necessary, but not necessarily sufficient, for endosomal / lysosomal trafficking of all antigens. Instead, the complete luminal domain of LAMPs has been shown to be required for trafficking of proteins to the lysosomal vesicle pathway.

[0008] However, even when the complete luminal domain and complete transmembrane / cytoplasmic tail of LAMP ("complete LAMP construct") are present, it has gradually been found that the effect of enhancing the immune response of a particular antigen is highly dependent on the specific sequences used in these constructs. In fact, it has been found that different antigen fragments of the same protein do not induce the same immune response when inserted into a complete LAMP construct. At some times, an antigen fragment generates an immune response, but at other times, it does not. These observations allow one to predict in advance which specific antigen sequences of a protein of interest will trigger an immune response that would be difficult to achieve with a complete LAMP construct.

[0009] Furthermore, in generating complete LAMP constructs, it has been repeatedly observed that an intact luminal domain is required for proper expression and processing of antigens. For example, in Godinho et al., PLoS ONE 9(6):9(6):e99887.doi:10.1371 / journal.pone.0099887, the authors reported that a complete and intact luminal domain is the minimal region required to target an antigen to lysosomes, and that fragments of the luminal domain do not work. See id on page 6.

[0010] Specifically, author Godinho showed that complete removal of the first luminal domain and some of the second luminal domain (i.e., the T1-Lum / gag construct) reduced both protein expression and antibody responses. Similarly, deletion of 25% of the first luminal domain but with an intact second luminal domain (i.e., T2-lum / gag) relatively increased both protein expression and antibody responses, but still less than those obtained with the complete LAMP construct.

[0011] Furthermore, the authors acknowledged that the ability to trigger an immune response depends on the specific antigens and epitopes used in these complete LAMP constructs. For example, on page 9, in the second column, the authors state, "Accordingly, previous studies have demonstrated that DNA vaccines producing Gag secreted as VLPs or in a soluble form induce different levels of T-cell and B-cell activation, which are also distinct from the responses induced by cytoplasmic Gag." However, as described in this literature, inserting antigen sequences between the complete luminal domain of the LAMP and the complete transmembrane / cytoplasmic domain of the LAMP would result in large polynucleotide sequences that would be too expensive to produce at a commercial level or would be impractical from a scientific point of view.

[0012] Therefore, there is a need to design new and improved LAMP constructs that can be used as vaccines to effectively treat, for example, allergies, infectious diseases, diabetes, hyperproliferative disorders and / or cancer. Furthermore, once improved, these new LAMP constructs can be used to generate antibodies. [Prior art documents] [Patent documents]

[0013] [Patent Document 1] U.S. Patent No. 5,633,234 [Patent Document 2] U.S. Patent No. 8,318,173 [Non-patent literature]

[0014] [Non-Patent Document 1] J.Immunol.166:5250-5257,2001 [Non-Patent Document 2] Fernandes et al.,2000,Eur.J.Immunol.30(8):2333-43 [Non-Patent Document 3] Godinho et al.,PLoS ONE 9(6):9(6):e99887.doi:10.1371 / journal.pone.0099887 Summary of the Invention [Problem to be solved by the invention]

[0015] This Summary is provided to introduce in a simplified form a selection of concepts further described below in the Detailed Description. This Summary is not intended to identify key or essential features of the claimed subject matter, nor is it intended to be used to limit the scope of the claimed subject matter. Other features, details, utilities, and advantages of the claimed subject matter will become apparent from the following Detailed Description, including the aspects illustrated in the accompanying drawings and defined in the appended claims.

[0016] It is an object of the present invention to provide novel constructs comprising specific fragments and / or variants of the LAMP domain ("improved LAMP constructs") that effectively present antigen(s) of interest to the immune system to generate enhanced immune responses. These improved LAMP constructs effectively target antigens to lysosomal / endosomal compartments where they are processed and presented to major histocompatibility complex (MHC) class II molecules for preferential stimulation of helper T cells and / or for antibody generation.

[0017] The improved LAMP constructs and methods described herein can induce an immune response in a subject. The immune response can be an immune response to an epitope of an antigen in the improved LAMP construct (e.g., a vaccine). The vaccine arms the immune system of a subject so that the immune system can detect and destroy anything in the subject that contains the antigen of the vaccine. The improved LAMP constructs and methods described herein can induce a Th1 immune response in a subject. A Th1 immune response can include the secretion of inflammatory cytokines (e.g., IFNγ, TNFα) by a subset of immune cells (e.g., antigen-specific T cells). In some cases, the inflammatory cytokines activate another subtype of immune cells (such as cytotoxic T cells) in the subject that can destroy anything that contains the antigen.

[0018] In some cases, the epitopes and / or antigens used in the improved LAMP constructs and methods described herein can be recognized by the subject's immune system, eliciting a Th1 immune response and releasing type I cytokines. A Th1 response can be initiated by the interaction between an epitope and a T cell, more specifically, a major histocompatibility complex (MHC) expressed by the T cell. For example, high affinity binding of an epitope to an MHC receptor can stimulate a Th1 response. The MHC receptor can be at least one of multiple types of MHC receptors. The MHC receptors involved in T cells can vary among individuals in a population.

[0019] In some cases, the immune response is a type 1 immune response. In some cases, the immune response is characterized by a ratio of type I cytokine production to type II cytokine production that exceeds 1. In some cases, the immune response is characterized by a ratio of type I cytokine production to type II cytokine production that is less than 1. In some cases, the immune response is characterized by a ratio of IFNγ production to IL-10 production that exceeds 1. In some cases, the immune response is characterized by a ratio of IFNγ production to IL-10 production that is less than 1.

[0020] Yet another object of the present invention is to provide an improved method for the treatment of cancer and / or hyperproliferative disorders by inducing an anti-tumor immune response through the stimulation of helper T cells. [Means for solving the problem]

[0021] The improved LAMP constructs described herein can also be used to treat allergies, such as food allergies (e.g., peanut allergens such as Ara H1, H2 and / or H3), or environmental allergens, such as pollen (tree pollens, such as CRY J1 or CRY J2), dog dander, cat saliva, or dust mites. Other diseases and / or disorders that can be treated using the improved LAMP constructs described herein include, for example, infectious diseases and diabetes.

[0022] The present invention further provides a nucleic acid molecule encoding any of the improved LAMP constructs described herein. The present invention also provides an improved LAMP construct comprising an antigen for generating antibodies. The improved LAMP construct can comprise a nucleic acid molecule operably linked to an expression control sequence. In a preferred embodiment, the improved LAMP construct is a vaccine vector suitable for vaccinating a patient. In another embodiment, the present invention provides a delivery vehicle comprising the improved LAMP construct for facilitating the introduction of a nucleic acid molecule encoding an antigen into a cell. The delivery vehicle can be lipid-based (e.g., a liposomal formulation), viral-based (e.g., comprising a viral protein that encapsulates the nucleic acid molecule), or cell-based.

[0023] In a preferred embodiment, the present invention provides an injectable composition comprising an improved LAMP construct comprising an antigen of interest for inducing an immune response (e.g., production of antibodies) in a mammal against the antigen. In a preferred embodiment, the vaccine generates a Th1 response preferentially over a Th2 response. The improved LAMP construct comprises at least one epitope of the antigen.

[0024] The present invention also provides cells comprising any of the improved LAMP constructs described herein that can be used to generate an immune response. In one embodiment, the cell is an antigen-presenting cell. The antigen-presenting cell can be a professional antigen-presenting cell (e.g., dendritic cell, macrophage, B cell, etc.) or an engineered antigen-presenting cell (e.g., a non-professional antigen-presenting cell engineered to express a molecule required for antigen presentation, e.g., an MHC class II molecule). The molecule required for antigen presentation can be derived from another cell, e.g., a natural cell, or can itself be engineered (e.g., mutated or modified to express a desired property, such as high or low affinity for an antigenic epitope). In one embodiment, the antigen-presenting cell does not express any costimulatory signal and the antigen is a self-antigen.

[0025] The present invention further provides a kit comprising a plurality of cells comprising any of the improved LAMP constructs described herein. At least two cells express different MHC class II molecules, and each cell comprises the same LAMP construct. In one embodiment, a kit is provided comprising an improved LAMP construct and cells for receiving a vector.

[0026] The present invention also provides a transgenic animal comprising at least one cell and / or at least one improved LAMP construct as described herein.The present invention also provides a transgenic animal comprising at least one cell as described herein.

[0027] The present invention further provides a method of generating an immune response to an antigen in an animal (e.g., a human or non-human vertebrate), comprising administering the above-described cells to the animal, wherein the cells express or can be induced to express an improved LAMP construct in the animal. In one embodiment, the cells contain MHC class II molecules that are compatible with the MHC proteins of the animal, such that the animal does not generate an immune response to the MHC class II molecules. In a preferred embodiment, the animal is a human.

[0028] In a further aspect, the present invention provides a method for inducing an immune response to an antigen, comprising administering to an animal, such as a human or non-human vertebrate, any of the improved LAMP constructs described herein.Preferably, the improved LAMP construct is infectious to the cells of the animal.For example, the improved LAMP construct can be a viral vector, such as a vaccinia improved LAMP construct.

[0029] Prime-boost protocols are also contemplated. For example, the present invention further provides a method of generating an immune response to an antigen in an animal, comprising priming the animal with an improved LAMP construct comprising an antigen as described herein, followed by at least one boosting of the animal with the antigen or a related antigen (e.g., a second antigen derived from the same or very similar protein sequence). A mixture of antigens can be used in either or both of the priming and boosting steps. The use of improved LAMP constructs for the priming step followed by the antigen boost step has been shown to generate very high titers, demonstrating the power of LAMP in enhancing antibody responses.

[0030] In a further embodiment, cells are obtained from a patient, an improved LAMP construct as described herein is introduced into the cells, and the cells or progeny of the cells are reintroduced into the patient. In one embodiment, the cells are stem cells capable of differentiating into antigen-presenting cells. Treatment of human patients and veterinary uses are specifically contemplated.

[0031] The present invention also includes a method for generating antibodies in a non-human vertebrate by injecting a non-human vertebrate with an improved LAMP construct comprising an antigen of interest as described herein, which is then efficiently presented to the immune system of the non-human vertebrate with the aid of the LAMP, resulting in the production of antibodies against the antigen.

[0032] Specifically, by combining the presentation of an antigen of interest with LAMP, the antigen is then efficiently transported to the cytoplasmic endosomal / lysosomal compartments, where it is processed and antigen-derived peptides are presented on the cell surface with the cooperation of major histocompatibility complex (MHC) class II molecules.

[0033] These generated antibodies are isolated from the vertebrate blood (as polyclonals) and further separated to generate monoclonal antibodies using standard techniques.

[0034] In a preferred embodiment, the genome of the non-human vertebrate comprises an introduced partial human immunoglobulin region, said introduced region comprising human immunoglobulin variable region locus coding sequences and non-coding sequences based on the endogenous immunoglobulin variable region locus of the non-human vertebrate. Preferably, the genome of the non-human vertebrate has at least some or all of the endogenous immunoglobulin region removed.

[0035] In a further preferred embodiment, production of human monoclonal antibodies in a non-human vertebrate requires that the host have at least one locus that expresses a human heavy chain immunoglobulin protein and one locus that expresses a human light chain immunoglobulin protein.

[0036] In some embodiments, the partially human immunoglobulin variable region locus is a human V H Coding sequences and endogenous V of non-human vertebrates H Region-based non-coding V H In these embodiments, the partially human immunoglobulin variable region locus further comprises human D and J gene coding sequences and non-coding D and J gene sequences based on the endogenous genome of the non-human vertebrate host.

[0037] In other embodiments, the immunoglobulin region is a human V L Coding sequences and endogenous V of non-human vertebrates L Region-based non-coding V LMore preferably, the human V L The introduced partially human immunoglobulin region comprising a coding sequence further comprises a human J gene coding sequence and a non-coding J gene sequence based on the endogenous genome of a non-human vertebrate host.

[0038] In certain embodiments, the vertebrate is a mammal, preferably the mammal is a rodent, such as a mouse or a rat. In other embodiments, the vertebrate is an avian, such as a chicken. Other non-human vertebrates include rabbits, llamas, camels, cows, guinea pigs, hamsters, dogs, cats, horses, non-human primates, anthropoids (e.g. monkeys or apes), monkeys (e.g. marmosets, baboons, rhesus monkeys), or apes (e.g. gorillas, chimpanzees, orangutans, gibbons).

[0039] In a further embodiment, the partially human immunoglobulin region is a human V H In another embodiment, the V gene coding region further comprises i) human D and J gene coding sequences, and ii) non-coding D and J genes and pre-DJ sequences based on the endogenous genome of a non-human vertebrate host. H The gene coding regions can be (at least in part) derived from other sources, e.g., they can be rationally or otherwise designed sequences, sequences that are a combination of human and other designed sequences, or sequences from other species, e.g., non-human primates.

[0040] In yet another particular embodiment, the partially human immunoglobulin region is a human V L In certain embodiments, the partially human immunoglobulin region comprises a human V gene coding sequence, and further comprises i) a human J gene coding sequence, and ii) a non-coding J gene sequence based on the endogenous genome of the non-human vertebrate host. H It further includes coding regions, human D and J gene coding sequences, as well as non-coding D and J gene and pre-DJ sequences based on the endogenous genome of a non-human vertebrate host.

[0041] The methods described herein can be used to produce and / or optimize antibodies, including fully human, humanized, and chimeric antibodies, for diagnostic and therapeutic applications. Hybridomas producing such antibodies are further objects of the present invention.

[0042] These and other aspects, objects and features are described in further detail below.

[0043] The objects and features of the present invention can be better understood with reference to the following detailed description and the accompanying drawings. [Brief description of the drawings]

[0044] [Figure 1] FIG. 1 illustrates a general scheme of the various types of improved LAMP constructs (identified as ILC-1, ILC-2, ILC-3, ILC-4, ILC-5 and ILC-6) that can be used as described herein. [Diagram 2] Figure 2A is a diagram showing the domains of the LAMP proteins as defined herein, and Figure 2B is a diagram defining the specific amino acid boundaries of these domains for human LAMP-1 (SEQ ID NO: 1), human LAMP-2 (SEQ ID NO: 2), human LAMP-3 (SEQ ID NO: 3), human LIMP-2 (SEQ ID NO: 4), human Endolyn (SEQ ID NO: 5), human Macrosialin (SEQ ID NO: 80), human LAMP-5 (SEQ ID NO: 93) and human LIMBIC (SEQ ID NO: 67). As described herein, the LAMP luminal domain, homology domain, transmembrane domain, cytoplasmic tail and signal sequence can be used to generate the improved LAMP constructs ILC-1, ILC-2, ILC-3, ILC-4, ILC-5 and ILC-6 as described herein. [Diagram 3]Figure 3 provides an alignment of LAMP-1 proteins found in other species compared to human LAMP-1 (SEQ ID NO: 1). Equivalent domains in these other species can be used to generate improved LAMP constructs as described herein and can be readily identified by comparing the domains identified for human LAMP-1 in Figures 2 and 3 with the alignment shown in Figure 3. [Figure 4] Figure 4 provides an alignment of LAMP-2 proteins found in other species compared to human LAMP-2 (SEQ ID NO: 2). Equivalent domains in these other species can be used to generate improved LAMP constructs as described herein and can be readily identified by comparing the domains identified for human LAMP-2 in Figures 2 and 4 with the alignment shown in Figure 4. [Diagram 5] Figure 5 provides an alignment of LAMP-3 proteins found in other species compared to human LAMP-3 (SEQ ID NO: 3). Equivalent domains in these other species can be used to generate improved LAMP constructs as described herein and can be readily identified by comparing the domains identified for human LAMP-3 in Figures 2 and 5 with the alignment shown in Figure 5. [Figure 6] Figure 6 provides an alignment of LIMP-2 proteins found in other species compared to human LIMP-2 (SEQ ID NO: 4). Equivalent domains in these other species can be used to generate improved LAMP constructs as described herein and can be readily identified by comparing the domains identified for human LIMP-2 in Figures 2 and 6 with the alignment shown in Figure 6. [Figure 7]Figure 7 provides an alignment of LIMBIC proteins found in other species compared to human LIMBIC (SEQ ID NO: 67). Equivalent domains in these other species can be used to generate improved LAMP constructs as described herein and can be readily identified by comparing the domains identified for human LIMBIC in Figures 2 and 7 with the alignment shown in Figure 7. [Figure 8] Figure 8 provides an alignment of Endolyn proteins found in other species compared to human Endolyn (SEQ ID NO: 5). Equivalent domains in these other species can be used to generate improved LAMP constructs as described herein and can be readily identified by comparing the domains identified for human Endolyn in Figures 2 and 8 with the alignment shown in Figure 8. [Figure 9] Figure 9 provides an alignment of human Macrosialin proteins found in other species compared to human Macrosialin (SEQ ID NO: 80). Equivalent domains in these other species can be used to generate improved LAMP constructs as described herein and can be readily identified by comparing the domains identified for human Macrosialin in Figures 2 and 9 with the alignment shown in Figure 9. [Figure 10] Figure 10 provides an alignment of LAMP-5 proteins found in other species compared to human LAMP-5 (SEQ ID NO: 93). Equivalent domains in these other species can be used to generate improved LAMP constructs as described herein and can be readily identified by comparing the domains identified for human LAMP-5 in Figures 2 and 10 with the alignment shown in Figure 10. [Figure 11]Figure 11 shows the results obtained when mice were immunized with HVEM-LAMP, HVEM, or LAMP on days 0, 7, and 14. On day 28, mice were bled and serum samples were isolated. HVEM-specific IgG was examined by ELISA. Data represent the geometric mean of antibody titers ± geometric SD, n=6. **p-value<0.01 [Figure 12] Figure 12 shows the results obtained when mice were immunized with HVEM-LAMP, HVEM, or LAMP on days 0, 7, and 14. On day 35, mice were boosted with 5 μg HVEM protein in the presence of alum adjuvant. Mice were bled on day 49 and serum samples were isolated. HVEM-specific IgG was examined by ELISA. Data represent the geometric mean of antibody titers ± geometric SD, n=6. ***p-value<0.001; ****p-value<0.0001. [Figure 13] FIG. 13 shows that LAMP alters the binding affinity of epitopes on CRD3 / 4 of HVEM. [Figure 14] Figure 14 confirms protein expression of the improved LAMP constructs tested. In each of Figures 14-17, the designations "complete LAMP construct", ILC-1, ILC-2, ILC-3 and ILC-4 correspond to the constructs shown in Figure 1. [Figure 15] FIG. 15 shows that the improved LAMP construct induces Th1 effector T cells that produce INFγ. [Figure 16] FIG. 16 shows that certain improved LAMP constructs (eg, ILC-4 as shown in FIG. 1) elicited significantly higher T cell responses against all survivin peptide pools. [Figure 17] FIG. 17 shows that CD4 T cells are the major source of IFNγ producing cells and the improved LAMP construct displays an increased CD4 effector memory cell population over the complete LAMP construct. [Figure 18] FIG. 18 shows that the improved LAMP construct generated stronger survivin-specific total IgG responses in BALB / c mice. [Figure 19] Figure 19 provides the amino acid sequence of each tested LAMP construct. The signal sequence of each construct is represented by lowercase and underlined letters, the survivin sequence is written in uppercase white letters on a black background, the luminal domain is written in italic capital letters, and the transmembrane / cytosolic domain is written in capital letters and shaded in grey, and in ILC-4, the second homology domain is in bold. Additional amino acids (LE and EF) can be included as part of the cloning linker. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0045] The present invention provides improved LAMP constructs and nucleic acids encoding them, which can be used to generate vaccines and / or to produce antibodies. The improved LAMP constructs can be used to modulate or enhance immune responses. In a preferred embodiment, the present invention provides a method of treating patients with allergies, infectious diseases, diabetes, cancer or hyperproliferative disorders by providing the improved LAMP constructs described herein. The improved LAMP constructs can also be used to produce antibodies in non-human vertebrates, and preferably non-human mammals.

[0046] definition The following definitions are provided for specific terms used in the description that follows.

[0047] As used in this specification and claims, the singular forms "a," "an," and "the" include plural referents unless the context clearly dictates otherwise. For example, the term "a cell" includes a plurality of cells, including mixtures thereof. The term "nucleic acid molecule" includes a plurality of nucleic acid molecules.

[0048] The term "comprising" as used herein is intended to mean that the improved LAMP constructs and methods include the recited elements but do not exclude other elements. "Consisting essentially of" when used to define the improved LAMP constructs and methods shall mean excluding any other elements of essential importance to the combination. Thus, an improved LAMP construct consisting essentially of the elements defined herein does not exclude trace contaminants from the isolation and purification methods, as well as pharma- ceutically acceptable carriers such as phosphate buffered saline, preservatives, etc. "Consisting of" means excluding trace elements of other components and more than substantial method steps for administering the improved LAMP constructs of the invention. Embodiments defined by each of these transition terms are within the scope of the invention.

[0049] The term "about" or "approximately" means within an acceptable range of a particular value as determined by one of ordinary skill in the art, which depends in part on how the value is measured or determined, e.g., the limitations of the measurement system. For example, "about" can mean within a range of up to 20%, preferably up to 10%, more preferably up to 5%, and even more preferably up to 1% of a given value. Alternatively, particularly with respect to biological systems or processes, the term can mean within 10-fold, preferably within 5-fold, and more preferably within 2-fold of a value. Unless otherwise specified, the term "about" means within an acceptable range of error of a particular value, e.g., ±1-20%, preferably ±1-10%, and more preferably ±1-5%.

[0050] When a range of values ​​is provided, it is understood that each intervening value between the upper and lower limit of that range, as well as any other stated or intervening value within that stated range, is encompassed within the invention. The upper and lower limits of these smaller ranges may be independently included in the smaller ranges and are encompassed within the invention, subject to the exclusion of any specific limit in the stated range. When the stated range includes one or both of the limits, ranges excluding both of those included limits are also included in the invention.

[0051] As used herein, the term "lysosomal / endosomal compartment" refers to a membrane-bound acidic vacuole that contains LAMP molecules in its membrane, hydrolases that function in antigen processing, and MHC class II molecules for antigen recognition and presentation. This compartment serves as a degradation site for foreign bodies internalized from the cell surface by any of a variety of mechanisms, including endocytosis, phagocytosis, and pinocytosis, and for intracellular materials delivered to the compartment by specialized autolytic events (de Duve, Eur. J. Biochem. 137:391, 1983). As used herein and in the claims, the term "endosome" encompasses lysosomes.

[0052] As used herein, "lysosome-associated organelle" refers to any organelle that contains a lysosome, including, but not limited to, MIIC, CIIV, melanosome, secretory granule, lytic granule, platelet dense granule, basophil granule, Virbeck granule, phagolysosome, secretory lysosome, etc. Preferably, such organelles lack mannose 6-phosphate receptors and contain LAMPs, but may or may not contain MHC class II molecules. For reviews, see, e.g., Blot and Griffiths, Nature Reviews, Molecular Cell Biology, 2002; Dell'Angelica, et al., The FASEB Journal 14:1265-1278, 2000.

[0053] As used herein, the terms "polynucleotide" and "nucleic acid molecule" are used interchangeably to refer to polymeric forms of nucleotides of any length. Polynucleotides may contain deoxyribonucleotides, ribonucleotides and / or their analogs. Nucleotides can have any three-dimensional structure and can perform any function, known or unknown. The term "polynucleotide" includes, for example, single-stranded, double-stranded and triple-helical molecules, genes or gene fragments, exons, introns, mRNA, tRNA, rRNA, ribozymes, antisense molecules, cDNA, recombinant polynucleotides, branched polynucleotides, aptamers, plasmids, vectors, isolated DNA of any sequence, isolated RNA of any sequence, nucleic acid probes, and primers. Nucleic acid molecules may also include modified nucleic acid molecules (e.g., containing modified bases, sugars, and / or internucleotide linkers).

[0054] As used herein, the term "peptide" refers to a compound of two or more subunit amino acids, amino acid analogs, or peptidomimetics. The subunits may be linked by peptide bonds or other bonds (e.g., esters, ethers, etc.).

[0055] The term "amino acid" as used herein refers to natural and / or unnatural or synthetic amino acids, including glycine and both D or L optical isomers, as well as amino acid analogs and peptidomimetics. A peptide of three or more amino acids is commonly called an oligopeptide if the peptide chain is short. If the peptide chain is long (e.g., more than about 10 amino acids), the peptide is commonly called a polypeptide or protein. The term "protein" encompasses the term "polypeptide", although a "polypeptide" may be shorter than a full-length protein.

[0056] As used herein, "LAMP polypeptide" refers to the mammalian lysosomal associated membrane proteins described herein, such as human LAMP-1, human LAMP-2, human LAMP-3, human LIMP-2, human Endolyn, human LIMBIC, human LAMP-5, or human Macrosialin, and orthologs (e.g., the LAMP proteins shown in Figures 3-10), and allelic variants.

[0057] As used herein, "expression" refers to the process by which a polynucleotide is transcribed into mRNA and / or translated into a peptide, polypeptide, or protein. If the polynucleotide is derived from genomic DNA, expression may include splicing of the mRNA transcribed from the genomic DNA.

[0058] As used herein, "under transcriptional control" or "operably linked" refers to expression (e.g., transcription or translation) of a polynucleotide sequence that is controlled by the appropriate juxtaposition of expression control elements and a coding sequence. In one aspect, a DNA sequence is "operably linked" to an expression control sequence if the expression control sequence controls and regulates the transcription of that DNA sequence.

[0059] As used herein, a "coding sequence" is a sequence which is transcribed and translated into a polypeptide when placed under the control of appropriate expression control sequences. The boundaries of the coding sequence are determined by a start codon at the 5' (amino) terminus and a translation stop codon at the 3' (carboxyl) terminus. Coding sequences can include, but are not limited to, prokaryotic sequences, cDNA from eukaryotic mRNA, genomic DNA sequences from eukaryotic (e.g., mammalian) DNA, and even synthetic DNA sequences. Polyadenylation signals and transcription termination sequences are usually located 3' to the coding sequence.

[0060] As used herein, two coding sequences "correspond" to each other if the sequences or their complementary sequences encode the same amino acid sequence.

[0061] As used herein, "signal sequence" refers to an endoplasmic reticulum translocation sequence. This sequence encodes a signal peptide that communicates with the cell to direct a polypeptide that is linked to the cell (e.g., via chemical bond) to an endoplasmic reticulum vesicular compartment, enter an exocytosis / endocytosis organelle, and deliver the polypeptide to either a cellular vesicular compartment, the cell surface, or secrete the polypeptide. This signal sequence is sometimes clipped off by the cell during protein maturation. Signal sequences are found associated with a variety of proteins native to prokaryotes and eukaryotes.

[0062] "Transport" as used herein refers to the movement or progression of a polypeptide encoded by an improved LAMP construct through cellular organelles or compartments on the route from the rough endoplasmic reticulum to the endosomal / lysosomal compartment, or through related organelles where antigen processing and binding to MHC II occurs.

[0063] As used herein, "improved LAMP construct" and "improved LAMP construct comprising an antigen" and "improved LAMP construct comprising an antigen of interest" are used interchangeably. Various configurations of improved LAMP constructs are shown in FIG. 1 as ILC1-ILC6. Furthermore, the use of "improved LAMP construct" encompasses both the polynucleotide sequence of the improved LAMP construct and the protein encoded by the polynucleotide sequence of the improved LAMP construct.

[0064] As used herein, an "improved LAMP construct delivery vehicle" is defined as any molecule or group of molecules or macromolecules (e.g., genes or gene fragments, antisense molecules, ribozymes, aptamers, etc.) that occur in association with an improved LAMP construct described herein that is capable of carrying the improved LAMP construct into a host cell.

[0065] As used herein, "improved LAMP construct delivery" or "improved LAMP construct transfer" refers to the introduction of an improved LAMP construct into a host cell, regardless of the method used for introduction. The introduced improved LAMP construct can be stably or transiently maintained in the host cell. Typically, stable maintenance requires that the introduced improved LAMP construct contains a replication origin compatible with the host cell or is integrated into a host cell replicon, such as an extrachromosomal replicon (such as a plasmid) or a nuclear or mitochondrial chromosome.

[0066] As used herein, "viral modified LAMP construct" refers to a virus or virus particle containing an improved LAMP construct that is delivered to a host cell either in vivo, ex vivo or in vitro. Examples of viral modified LAMP constructs include, but are not limited to, adenoviral vectors, adeno-associated virus vectors, retroviral vectors, and the like. In an embodiment in which gene transfer is mediated by an adenoviral vector, the improved LAMP construct comprises an adenoviral genome or a portion thereof, and a selected non-adenoviral gene associated with an adenoviral capsid protein.

[0067] As used herein, "adenovirus-mediated gene transfer" or "adenovirus transduction" refers to a method in which an improved LAMP construct is introduced into a host cell by adenovirus entering the cell. Preferably, the improved LAMP construct can replicate and / or integrate and transcribe within the cell.

[0068] As used herein, an "adenovirus particle" refers to an individual adenovirus virion that includes an outer capsid and an improved LAMP construct, which further includes an adenovirus envelope protein. The adenovirus envelope protein may be modified to include a fusion polypeptide that contains a polypeptide ligand covalently linked to a viral protein, for example, to target the adenovirus particle to a specific cell and / or tissue type.

[0069] As used herein, the term "administration" or "immunization" or "injection" of an improved LAMP construct refers to transducing, transfecting, microinjecting, electroporating, or firing a cell with the improved LAMP construct. In some embodiments, the improved LAMP construct is introduced into a target cell by contacting the target cell with a delivery cell (e.g., by cell fusion or by lysing the delivery cell when it is in close proximity to the target cell).

[0070] The phrase "prime-boost" as used herein refers to the use of an improved LAMP construct as described herein used to prime a T cell response, followed by the use of a second improved LAMP construct containing an antigen, a DNA vaccine containing an antigen or a recombinant antigen to boost said response. These heterologous prime-boost immunizations induce higher and broader immune responses than can be achieved by priming and boosting with the same vector. Priming with an improved LAMP construct containing an antigen elicits memory cells, and the boost step expands the memory response. Preferably, the use of two different agents does not provoke a reaction against each other and therefore does not interfere with each other's activity. Mixtures of antigens are specifically contemplated in the prime and / or boost steps. Boosting may be performed one or more times.

[0071] "Hybridization" as used herein refers to a reaction in which one or more polynucleotides react to form a complex stabilized through hydrogen bonding between the bases of the nucleotide residues. The hydrogen bonds may occur by Watson-Crick base pairing, Hoogstein binding, or any other sequence-specific manner. The complex may include two strands forming a duplex structure, three or more strands forming a multistranded complex, a single self-hybridizing strand, or any combination of these. A hybridization reaction may constitute a step in a more extensive method, such as the initiation of a PCR reaction, or the enzymatic cleavage of a polynucleotide by a ribozyme.

[0072] As used herein, a polynucleotide or polynucleotide region (or polypeptide or polypeptide region) having a particular percentage (e.g., at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, at least about 99%) "sequence identity" to another sequence means that that percentage of bases (or amino acids) are the same when the two sequences are compared, when maximally aligned using software programs routine in the art.

[0073] Two sequences are "substantially homologous" or "substantially similar" if at least about 50%, at least about 60%, at least about 70%, at least about 75%, preferably at least about 80%, and most preferably at least about 90 or 95% of the nucleotides match over a defined length of the DNA sequence. Similarly, two sequences are "substantially homologous" or "substantially similar" if at least about 50%, at least about 60%, at least about 66%, at least about 70%, at least about 75%, preferably at least about 80%, and most preferably at least about 90 or 95% of the amino acid residues of the polypeptide match over a defined length of the polypeptide sequence. Substantially homologous sequences can be identified by comparing sequences using standard software available in sequence databanks. Substantially homologous nucleic acid sequences can also be identified, for example, in Southern hybridization experiments under stringent conditions defined for that particular system. Defining appropriate hybridization conditions is within the skill of the art. For example, stringent conditions are hybridization in 5xSSC and 50% formamide at 42°C, and washing in 0.1xSSC and 0.1% sodium dodecyl sulfate at 60°C. Further examples of stringent hybridization conditions include: incubation temperature of about 25°C to about 37°C; hybridization buffer concentration of about 6xSSC to about 10xSSC; formamide concentration of about 0% to about 25%; and washing solution of about 6xSSC. Examples of moderate hybridization conditions include: incubation temperature of about 40°C to about 50°C; buffer concentration of about 9xSSC to about 2xSSC; formamide concentration of about 30% to about 50%; and washing solution of about 5xSSC to about 2xSSC. Examples of highly stringent conditions include: an incubation temperature of about 55° C. to about 68° C.; a buffer concentration of about 1×SSC to about 0.1×SSC; a formamide concentration of about 55% to about 75%; and a wash solution of about 1×SSC, 0.1×SSC, or deionized water.Typically, hybridization incubation times range from 5 minutes to 24 hours with one, two or more washing steps, with wash incubation times of about 1, 2, or 15 minutes. SSC is 0.15M NaCl and 15mM citrate buffer. It will be appreciated that equivalents of SSC using other buffer systems can be used. Similarity can be verified by sequencing, but preferably or alternatively, can also be verified by function (e.g., ability to transport to endosomal compartments) using an assay appropriate for the particular domain in question.

[0074] The terms "percent sequence similarity", "percent sequence identity" and the like generally refer to the degree of identity or correspondence between different nucleotide sequences of nucleic acid molecules or amino acid sequences of polypeptides that may or may not share a common evolutionary origin. (See Reeck et al., supra). Sequence identity can be determined using any of a number of publicly available sequence comparison algorithms, such as BLAST, FASTA, DNA Strider, GCG (Genetics Computer Group, Program Manual for the GCG Package, Version 7, Madison, Wisconsin).

[0075] To determine the percent identity between two amino acid sequences or two nucleic acid molecules, the sequences are aligned for optimal comparison. The percent identity between two sequences is a function of the number of identical positions shared by these sequences (i.e., percent identity=number of identical positions / total number of positions (e.g., overlapping positions)×100). In one embodiment, the two sequences are the same length or approximately the same length. The percent identity between two sequences can be determined using a similar approach as described below, with or without allowing gaps. In calculating percent sequence identity, perfect matches are usually counted.

[0076] Determining percent identity between two sequences can be achieved using a mathematical algorithm. A non-limiting example of a mathematical algorithm used to compare two sequences is the algorithm of Karlin and Altschul, Proc. Natl. Acad. Sci. USA 1990, 87: 2264, as modified by Karlin and Altschul, Proc. Natl. Acad. Sci. USA 1993, 90: 5873-5877. Such an algorithm is incorporated into the NBLAST and XBLAST programs of Altschul et al, J. Mol. Biol. 1990; 215: 403. BLAST nucleotide searches can be performed with the NBLAST program, score = 100, word length = 12, to obtain nucleotide sequences homologous to the sequences of the present invention. BLAST protein searches can be performed with the XBLAST program, score = 50, word length = 3, to obtain amino acid sequences homologous to the protein sequences of the present invention. To obtain gapped alignments for comparison, one can use Gapped BLAST as described in Altschul et al, Nucleic Acids Res. 1997, 25:3389. Alternatively, PSI-Blast can be used to perform an iterated search that detects distant relationships between molecules. See Altschul et al. (1997) supra. When using BLAST, Gapped BLAST, and PSI-Blast programs, the default parameters of the respective programs (e.g., XBLAST and NBLAST) can be used. See WorldWideWeb at ncbi.nlm.nih.gov / BLAST / .

[0077] Another non-limiting example of a mathematical algorithm utilized for comparing sequences is the algorithm of Myers and Miller, CABIOS 1988;4:1 1-17. Such an algorithm is incorporated into the ALIGN program (version 2.0), which is part of the GCG sequence alignment software package. When utilizing the ALIGN program for comparing amino acid sequences, a PAM120 weight residue table, a gap length penalty of 12, and a gap penalty of 4 can be used.

[0078] In a preferred embodiment, the percent identity between two amino acid sequences is determined using the algorithm of Needleman and Wunsch (J. Mol. Biol. 1970, 48:444-453) as incorporated into the GAP program of the GCG software package (Accelrys, Burlington, MA; available on the WorldWideWeb at accelrys.com), using either a Blossum 62 matrix or a PAM250 matrix, gap weights of 16, 14, 12, 10, 8, 6, or 4, and length weights of 1, 2, 3, 4, 5, or 6. In yet another preferred embodiment, the percent identity between two nucleotide sequences is determined using the GAP program of the GCG software package, using a NWSgapdna.CMP matrix, gap weights of 40, 50, 60, 70, or 80, and length weights of 1, 2, 3, 4, 5, or 6. A particularly preferred set of parameters (and parameters that can be used if the practitioner is unsure which parameters must be applied to determine whether a molecule is within the sequence identity or homology limits of the invention) uses a gap open penalty of 12, a gap extension penalty of 4, and a frameshift gap penalty of 5, with a Blossum 62 scoring matrix.

[0079] Another non-limiting example of a method for determining percent identity is to use software programs such as those described in Current Protocols In Molecular Biology (FM Ausubel et al., eds., 1987) Supplement 30, section 7.7.18, Table 7.7.1. Preferably, default parameters are used for the alignment. A preferred alignment program is BLAST using default parameters. In particular, preferred programs are BLASTN and BLASTP using the following default parameters: Genetic code=standard; Filter=none; Strand=both; Cutoff=60; Expectation=10; Matrix=BLOSUM62; Descriptions=50 sequences; Sort by=HIGH SCORE; Database=non-redundant, GenBank+EMBL+DDBJ+PDB+GenBank CDS translations+SwissProtein+SPupdate+PIR. Details of these programs can be found at the following internet address: http: / / www.ncbi.nlm.nih.gov / cgi-bin / BLAST.

[0080] Statistical analysis of the characteristics described herein can be performed by standard tests, such as t-tests, ANOVA, or chi-square tests. Typically, statistical significance is measured to a level of p=0.05 (5%), more preferably p=0.01, p=0.001, p=0.0001, p=0.000001.

[0081] "Conservatively modified variants" of domain sequences can also be provided. With respect to a particular nucleic acid sequence, conservatively modified variants refer to nucleic acids that code identical or essentially identical amino acid sequences, or, if the nucleic acid does not code for an amino acid sequence, essentially identical sequences. Specifically, degenerate codon substitutions can be achieved by generating sequences in which the third position of one or more selected (or all) codons is replaced with mixed base and / or deoxyinosine residues (Batzer, et al., 1991, Nucleic Acid Res. 19:5081; Ohtsuka, et al., 1985, J. Biol. Chem. 260:2605-2608; Rossolini et al., 1994, Mol. Cell. Probes 8:91-98).

[0082] The terms "biologically active fragment," "biologically active form," "biologically active equivalent," and "functional derivative" of a wild-type protein have a biological activity that is at least substantially equal to (e.g., not significantly different from) the biological activity of the wild-type protein as measured using an assay suitable for detecting the activity.

[0083] As used herein, "in vivo" nucleic acid delivery, nucleic acid transfer, nucleic acid therapy, and the like refers to the direct introduction of an improved LAMP construct into the body of an organism, such as a human or non-human mammal, whereby the improved LAMP construct is introduced into the cells of such an organism in vivo.

[0084] The term "in situ" as used herein refers to in vivo nucleic acid delivery in which the improved LAMP construct is in close proximity to the target cells (e.g., the nucleic acid is not administered systemically). For example, in situ delivery methods include, but are not limited to, injecting the improved LAMP construct directly into the site (e.g., into a tissue such as a tumor or myocardium), contacting the improved LAMP construct with a cell or tissue(s) via a direct surgical field, or delivering the improved LAMP construct to the site using a medical access device such as a catheter.

[0085] The term "isolated" or "purified" as used herein means that a polynucleotide, peptide, polypeptide, protein, antibody, or fragment thereof is separated (or substantially free) from the cellular and other components with which it is normally associated in nature. For example, with respect to the improved LAMP construct, an isolated polynucleotide is one that is separated from the 5' and 3' sequences with which it is normally associated with the chromosome. As will be apparent to one of skill in the art, a non-natural polynucleotide, peptide, polypeptide, protein, antibody, or fragment thereof does not require "isolation" to distinguish it from its natural counterpart. Substantially free or substantially purified means that at least 50%, preferably at least 70%, more preferably at least 80%, and even more preferably at least 90% of the population is free of components with which it is naturally associated.

[0086] As used herein, "target cell" or "recipient cell" refers to an individual cell or cells that are desired to be or have been recipients of the improved LAMP construct described herein. The term is also intended to include the progeny of a single cell, which may not necessarily be completely identical (in morphology or genome or total DNA complement) to the original parent cell due to natural, accidental, or deliberate mutations. Target cells may be in contact with other cells (e.g., cells of a tissue) or circulating within the body of an organism.

[0087] As used herein, a "subject" is a vertebrate, preferably a mammal, more preferably a human. Mammals include, but are not limited to, murines, simians, humans, farm animals, sports animals, and pets. In other preferred embodiments, the "subject" is a rodent (e.g., rat, mouse, rabbit, llama, camel, cow, guinea pig, hamster, dog, cat, horse, non-human primate, simian (e.g., monkey or ape), monkey (e.g., marmoset, baboon, rhesus), or ape (e.g., gorilla, chimpanzee, orangutan, gibbon). In other embodiments, mammals (e.g., murine, primate, porcine, canine, or lagomorph) that are conventionally used as models for demonstrating therapeutic effects in non-human mammals, particularly humans, may be used.

[0088] The terms "cancer," "neoplasm," and "tumor" are used interchangeably, either in the singular or plural, to refer to cells that have undergone malignant transformation that renders the host organism pathological. Transformation of primary cancer cells renders the host organism pathological. Primary cancer cells (i.e., cells obtained from near the site of malignant transformation) are readily distinguishable from noncancerous cells by well-established techniques, particularly histological examination. The definition of cancer cells as used herein includes not only primary cancer cells, but any cells derived from a cancer cell ancestor. This includes metastasized cancer cells, and in vitro cultures and cell lines derived from cancer cells. When referring to types of cancer that typically manifest as solid tumors, a "clinically detectable" tumor is one that is detectable based on the tumor mass, for example, by procedures such as CAT scan, MR imaging, X-ray, ultrasound, or palpation, and / or is detectable due to the expression of one or more cancer-specific antigens in a sample obtained from a patient.

[0089] In a preferred embodiment, the cancer (including all advanced stages including hyperplasia) is adenocarcinoma, sarcoma, skin cancer, melanoma, bladder cancer, brain cancer, breast cancer, uterine cancer, ovarian cancer, prostate cancer, lung cancer (including but not limited to NSCLC, SCLC, squamous cell carcinoma), colorectal cancer, anal cancer, rectal cancer, cervical cancer, liver cancer, head and neck cancer, oral cancer, salivary gland cancer, esophageal cancer, pancreatic cancer, pancreatic ductal adenocarcinoma (PDA), renal cancer, gastric cancer, renal cancer, multiple myeloma or brain cancer.

[0090] The improved LAMP constructs described herein can also be used to treat allergies, such as food allergies (e.g., peanut allergens such as Ara H1, H2 and / or H3), or environmental allergens, such as pollen (tree pollens, such as CRY J1 or CRY J2), dog dander, cat saliva, or dust mites. Other diseases and / or disorders include, for example, infectious diseases and diabetes.

[0091] The term "pharmaceutical acceptable carrier" as used herein includes any of the standard pharmaceutical carriers, such as phosphate buffered saline, water, and emulsions, such as oil / water or water / oil emulsions, and various types of wetting agents. The composition containing the improved LAMP construct may also include stabilizers and preservatives. For examples of carriers, stabilizers, and adjuvants, see Martin Remington's Pharm.Sci., 15th Ed. (Mack Publ.Co., Easton (1975)).

[0092] When an improved LAMP construct is introduced into a cell, the cell is "transformed," "transduced," or "transfected" by such nucleic acid. The transforming DNA may or may not be integrated (covalently linked) into the chromosomal DNA that constitutes the genome of the cell. For example, in prokaryotes, yeast, and mammalian cells, the improved LAMP construct may be maintained in an episomal element, such as a plasmid. In eukaryotic cells, a stably transformed cell is one in which the improved LAMP construct is integrated into the chromosome and inherited by daughter cells through chromosome replication. This stability is demonstrated by the ability of the eukaryotic cell to establish a cell line or clone consisting of a population of daughter cells that contain the improved LAMP construct. A "clone" is a population of cells derived from a single cell or common ancestor by mitosis. A "cell line" is a clone of a primary cell that is capable of stable growth in vitro for many generations (e.g., at least about 10 generations).

[0093] As used herein, an "effective amount" is an amount sufficient to affect a beneficial or desired outcome, such as an effective amount of transfer and / or expression of an improved LAMP construct and / or an effective amount of achieving a desired therapeutic endpoint. An effective amount can be administered in one or more administrations, applications or dosages. In one embodiment, an effective amount of an improved LAMP construct is an amount sufficient to transform / transduce / transfect at least one cell in a cell population comprising at least two cells.

[0094] As used herein, a "therapeutically effective amount" is used herein to mean an amount sufficient to prevent, correct, and / or normalize an abnormal physiological response. In one embodiment, a "therapeutically effective amount" is an amount sufficient to reduce, for example, a clinically significant feature of a pathology, such as an allergic reaction, tumor mass size, antibody production, cytokine production, fever, or white blood cell count, by at least about 30 percent, more preferably at least 50 percent, and most preferably at least 90 percent.

[0095] An "antibody" is an immunoglobulin, including antibodies and fragments thereof, that bind to a specific antigen. The term includes polyclonal, monoclonal, and chimeric antibodies (e.g., bispecific antibodies). An "antibody combining site" is the structural portion of an antibody molecule comprised of the variable and hypervariable regions of the heavy and light chains that specifically binds to an antigen. Exemplary antibody molecules include intact immunoglobulin molecules, substantially intact immunoglobulin molecules, and Fab, Fab', F(ab') and / or F(ab') fragments. 2 and F(v) portions, which are preferred for use in the therapeutic methods described herein. Thus, the term antibody encompasses not only whole antibody molecules, but also antibody fragments, and variants of antibodies and antibody fragments, including derivatives such as fusion proteins. Examples of molecules described by the term "antibody" in this application include, but are not limited to, single chain Fv (scFv), Fab fragments, Fab' fragments, F(ab') fragments, and F(ab') fragments. 2 , disulfide-linked Fv (sdFv), Fv, and fragments comprising or consisting of either a VL or VH domain. As used herein, the term "single-chain Fv" or "scFv" refers to a polypeptide comprising the VL domain of an antibody linked to the VH domain of an antibody. See Carter (2006) Nature Rev. Immunol. 6:243.

[0096] Additionally, antibodies of the present invention include, but are not limited to, monoclonal, multispecific, bispecific, human, humanized, murine, or chimeric antibodies, single chain antibodies, camelid antibodies, Fab fragments, F(ab') fragments, anti-idiotypic (anti-Id) antibodies (including, for example, anti-Id antibodies to an antibody of the present invention), domain antibodies, and epitope-binding fragments of any of the above. The immunoglobulin molecules of the present invention can be of any type (e.g., IgG, IgE, IgM, IgD, IgA, and IgY), class (e.g., IgG1, IgG2, IgG3, IgG4, IgA1, and IgA2) or subclass of immunoglobulin molecule.

[0097] Most preferably, the antibody is a human antibody. As used herein, "human" antibody includes antibodies having the amino acid sequence of a human immunoglobulin, including antibodies isolated from human immunoglobulin libraries and xenomice or other organisms genetically engineered to produce human antibodies. The improved LAMP constructs described herein can be used in combination with known techniques for producing human antibodies and human monoclonal antibodies, as described in the exemplary protocols. See, e.g., PCT Publication Nos. WO 98 / 24893; WO 92 / 01047; WO 96 / 34096; WO 96 / 33735; European Patent No. 0598877; U.S. Patent Nos. 5,413,923; 5,625,126; 5,633,425; 5,569,825; 5,661,016; 5,545,806; 5,814,318; 5,885,793; 5,916,771; and 5,939,598; as well as Lonberg and Huszar, Int. Rev. Immunol. 13:65-93 (1995).

[0098] Human or "humanized" chimeric monoclonal antibodies can be produced using improved LAMP constructs in combination with the techniques described herein or other techniques known in the art. For example, standard methods for producing chimeric antibodies are known in the art. For reviews, see the following references: Morrison, Science 229:1202 (1985); Oi et al., BioTechniques 4:214 (1986); Cabilly et al., U.S. Pat. No. 4,816,567; Taniguchi et al., EP 171496; Morrison et al., EP 173494; Neuberger et al., WO 8601533; Robinson et al., WO 8702671; Boulianne et al., Nature 312:643 (1984); Neuberger et al., Nature 314:268 (1985).

[0099] The antibody of the present invention can be monovalent, bivalent, trivalent or multivalent. For example, monovalent scFv can be multimerized chemically or by binding with another protein or substance. scFv fused with hexahistidine tag or Flag tag can be multimerized using Ni-NTA agarose (Qiagen) or anti-Flag antibody (Stratagene, Inc.). Furthermore, the improved LAMP construct can be used to generate monospecific, bispecific, trispecific or greater multispecificity against the encoded antigen(s) contained in the improved LAMP construct. See, e.g., PCT publications WO 93 / 17715; WO 92 / 08802; WO 91 / 00360; WO 92 / 05793; Tutt, et al., J. Immunol. 147:60-69 (1991); U.S. Patent Nos. 4,474,893; 4,714,681; 4,925,648; 5,573,920; 5,601,819; Kostelny et al., J. Immunol. 148:1547-1553 (1992).

[0100] An "epitope" is a structure, usually a short peptide sequence or oligosaccharide, that is specifically recognized or specifically bound by components of the immune system. It has been shown that T cell epitopes are generally linear oligopeptides. Two epitopes correspond to each other if they can be specifically bound by the same antibody. Two epitopes correspond to each other if both can be bound to the same B cell receptor or the same T cell receptor, and the binding of one antibody to that epitope substantially prevents binding by the other epitope (e.g., less than about 30%, preferably less than about 20%, more preferably less than about 10%, 5%, 1%, or about 0.1% of other epitopes are bound). In the present invention, multiple epitopes can constitute an antigen.

[0101] The term "antigen" or "antigen of interest" as used herein encompasses polypeptide sequences encoded by polynucleotide sequences cloned into the improved LAMP construct that are used to elicit an innate or adaptive immune response. "Antigen" encompasses both single antigens and multiple antigen sequences (derived from the same or different proteins) cloned into the improved LAMP construct.

[0102] As used herein, the term "antigen presenting cell" includes any cell that presents on its surface an antigen associated with a major histocompatibility complex molecule or a portion thereof, or one or more non-classical MHC molecules or portions thereof. Examples of suitable APCs are described in detail below, and include, but are not limited to, whole cells such as macrophages, dendritic cells, B cells, hybrid APCs, and foster antigen presenting cells.

[0103] As used herein, "engineered antigen-presenting cell" refers to an antigen-presenting cell that has a non-natural molecular moiety on its surface.For example, such cells may not have a natural costimulatory substance on their surface, may have an additional artificial costimulatory substance on their surface in addition to a natural costimulatory substance, or may express a non-natural class II molecule on their surface.In a preferred embodiment, the engineered antigen-presenting cell has an antigen expressed by an improved LAMP construct on its surface.

[0104] As used herein, "immune effector cells" refers to cells that can bind to antigens and mediate immune responses. These cells include, but are not limited to, T cells, B cells, monocytes, macrophages, NK cells, and cytotoxic T lymphocytes (CTLs), such as CTL lineages, CTL clones, and CTLs from tumors, inflammatory, or other infiltrates.

[0105] As used herein, "partially human" refers to a nucleic acid having sequences from both human and non-human vertebrates. In the context of a partially human sequence, the partially human nucleic acid has sequences based on human immunoglobulin coding regions and non-coding sequences of endogenous immunoglobulin regions of a non-human vertebrate. When used in reference to endogenous non-coding sequences from a non-human vertebrate, the term "based on" refers to sequences that correspond to non-coding sequences and share a relatively high degree of homology with non-coding sequences of endogenous loci of a host vertebrate, e.g., a non-human vertebrate from which an ES cell is derived. Preferably, the non-coding sequences share at least 80%, more preferably 90%, homology with the corresponding non-coding sequences found in the endogenous loci of a non-human vertebrate host cell into which the partially human molecule containing the non-coding sequences is introduced.

[0106] The term "immunoglobulin variable region" as used herein refers to a nucleotide sequence that encodes all or a portion of the variable region of an antibody molecule, or all or a portion of the regulatory nucleotide sequence that controls the expression of an antibody molecule. The immunoglobulin region of a heavy chain may include, but is not limited to, all or a portion of the V, D, J, and switch regions, including introns. The immunoglobulin region of a light chain may include, but is not limited to, the V and J regions, their upstream adjacent sequences, and introns associated with or adjacent to the light chain constant region gene.

[0107] By "transgenic animal" is meant a non-human animal, usually a mammal, that has an exogenous nucleic acid sequence present in some of its cells as an extrachromosomal element or stably integrated into the germline DNA (i.e., into the genomic sequence of most or all of its cells). In producing transgenic animals containing human sequences, a partially human nucleic acid is introduced into the germline of such a transgenic animal according to methods well known in the art, for example, by genetic manipulation of the host animal's embryos or embryonic stem cells.

[0108] "Vector" includes plasmids and viruses and any DNA or RNA molecule, whether autonomously replicating or not, that can be used to transform or transfect cells.

[0109] " Genetic modification " as used herein refers to any addition, deletion or disruption to the normal nucleotide of cells.Art-recognized methods include virus-mediated gene transfer, liposome-mediated transfer, transformation, transfection and transduction, for example, the improved LAMP construct based on DNA viruses such as adenovirus, adeno-associated virus and herpes virus, and the use of retrovirus-based vectors.

[0110] The practice of the present invention employs conventional molecular biology, microbiology, and recombinant DNA techniques within the skill of the art, unless otherwise specified. Such techniques are fully explained in the literature. See, for example, Maniatis, Fritsch & Sambrook, In Molecular Cloning: A Laboratory Manual (1982); DNA Cloning: A Practical Approach, Volumes I and II (DN Glover, ed., 1985); Oligonucleotide Synthesis (MJ Gait, ed., 1984); Nucleic Acid Hybridization (B.D. Hames & S.J. Higgins, eds., 1985); Transcription and Translation (B.D. Hames & S.I. Higgins, eds., 1984); Animal Cell Culture (R.I. Freshney, ed., 1986); Immobilized Cells and Enzymes (IRL Press, 1986); B. Perbal, A Practical Guide to Molecular Cloning (1984).

[0111] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention belongs. All publications mentioned herein are incorporated by reference for the purpose of describing and disclosing devices, formulations, and methodologies that can be used in connection with the invention described herein.

[0112] LAMP constructs LAMP-1, as deduced from a cDNA clone (Chen, et al., J. Biol. Chem. 263:8754, 1988), consists of a polypeptide core of approximately 382 amino acids with a large (346 residues) luminal amino-terminal domain followed by a 24-residue hydrophobic transmembrane region and a short (12 residues) carboxyl-terminal cytoplasmic tail. See Figures 2A and 2B. The luminal domain is heavily glycosylated and substituted with approximately 20 asparagine-linked complex-type oligosaccharides and consists of two approximately 160-residue "homologous domains" separated by a proline / serine-rich hinge region. Each of these "homologous domains" contains four evenly spaced cysteine ​​residues that are disulfide-bonded to form four 36-38 residue loops symmetrically arranged in both halves of the luminal domain (Arterburn, et al., J. Biol. Chem. 265:7419, 1990; see also Chen, et al., J. Biol. Chem. 25:263(18):8754-8, 1988). Figure 2A shows a schematic of the conserved domains between LAMP-1, LAMP-2, LAMP-3, Endolyn, LIMBIC, LAMP5, or Macrosialin.

[0113] The previously reported LAMP construct contains the following elements in this particular arrangement:

[0114] (a) the complete luminal domain of the LAMP-1 protein, the antigen, and the complete transmembrane / cytoplasmic tail of the LAMP-1 protein; or

[0115] (b) Antigen and the complete transmembrane / cytoplasmic tail of the LAMP-1 protein. In example (a), the antigen sequence is inserted between the complete luminal domain of the LAMP-1 protein and the LAMP-1 complete transmembrane domain / cytoplasmic tail. Both constructs have been shown to successfully target the antigen sequence to lysosomes / endosomes and are referred to as "complete LAMP constructs" as shown in FIG. 1, in comparison to the improved LAMP constructs ILC1-ILC6 described herein. The improved LAMP constructs described herein do not include the complete LAMP constructs described in the prior art.

[0116] Although it has been widely reported in the literature that fragments smaller than the complete luminal domain of LAMP-1 are not effective in generating a robust immune response (see, e.g., Godinho et al.), the present inventors have unexpectedly discovered that certain fragments in certain configurations do in fact effectively present antigens to the immune system and generate robust immune responses, including the generation of distinct repertoires of antibodies. For example, the present inventors have determined that the minimal LAMP luminal domain fragment effective in generating a robust immune response is not the complete luminal domain (as has been widely reported in the literature), but rather a single homologous domain of the luminal domain of the LAMP protein.

[0117] For example, the construct may not include a complete luminal domain, but may instead include a single homologous domain of the luminal domain of a LAMP protein. As used herein, a "homologous domain" includes at least four evenly spaced cysteine ​​residues as shown in Figures 3-10. The presence of these cysteines is labeled 1, 2, 3, and 4 in each homologous domain as shown in Figures 3-10 (five cysteines are identified in LIMP-2 and Macrosialin, six cysteines are identified in LIMBIC, and eight cysteines are identified in Endolyn), and is defined herein as a "cysteine-conserved fragment." Additional amino acids can be included at either the N-terminus and / or C-terminus of the cysteine-conserved fragment to generate a complete homologous domain of a LAMP protein. These additional added amino acids may be from the homologous domain from which the cysteine-conserved fragment is derived, or may be from other LAMP protein homologous domains. Thus, as used herein, a LAMP homology domain comprises and / or consists of one cysteine-conserved fragment. At least two LAMP homology domains constitute the luminal domain of LAMP-1, LAMP-2, LAMP-3, or Endolyn.

[0118] Specifically, in a preferred embodiment, the improved LAMP construct comprises at least one antigen of interest fused to the N-terminus of the luminal domain of a LAMP protein, at least one homologous domain of a LAMP protein, or at least one cysteine-conserved fragment of a LAMP protein. See, for example, ILC-2 and ILC-6 in FIG. 1. In a preferred embodiment, these constructs further comprise a transmembrane domain of a LAMP protein, and / or a cytosolic tail of a LAMP protein. In another preferred embodiment, when the antigen comprises a transmembrane domain, the transmembrane domain of a LAMP protein and / or the cytosolic tail of a LAMP protein is not required. In a preferred embodiment, two homologous domains are included in the improved LAMP construct (e.g., ILC-1 in FIG. 1). In a further preferred embodiment, the two homologous domains are derived from LAMP-1, LAMP-2, LAMP-3, or Endolyn proteins. Alternatively, the two homologous domains are derived from different LAMP proteins. These constructs that comprise two homologous domains may also include a LAMP hinge domain. Since fragments of the luminal domain have not been reported to be effective in generating a robust immune response, the improved LAMP constructs described in this paragraph are unexpected in view of the prior art in which the antigen is always located between the complete luminal LAMP-1 domain and the complete LAMP-1 transmembrane / cytoplasmic tail.

[0119] In another preferred embodiment, the improved LAMP construct comprises at least one antigen of interest fused to the C-terminus of a single homologous domain of a LAMP protein or a single cysteine-conserved fragment of a LAMP protein. See, for example, ILC-3 and ILC-5 in FIG. 1. In a preferred embodiment, these constructs further comprise a transmembrane domain of a LAMP protein and / or a cytosolic tail of a LAMP protein. In another preferred embodiment, when the antigen comprises a transmembrane domain, the transmembrane domain of a LAMP protein and / or the cytosolic tail of a LAMP protein are not required. The improved LAMP construct described in this paragraph is unexpected in view of the prior art described above.

[0120] In another preferred embodiment, the improved LAMP construct comprises at least one antigen of interest fused between a first homologous domain of a LAMP protein and a second homologous domain of a LAMP protein (or between at least two cysteine-conserved fragments). See, for example, ILC-4 in FIG. 1. In a preferred embodiment, these constructs further comprise a transmembrane domain of a LAMP protein and / or a cytosolic tail of a LAMP protein. In a preferred embodiment, the two homologous domains are derived from a LAMP-1, LAMP-2, LAMP-3, or Endolyn protein. In these constructs, the antigen may be located in the LAMP hinge region. Alternatively, two homologous domains from two different LAMP proteins may be used. This arrangement of at least one antigen of interest fused between two LAMP homologous domains (including cysteine-conserved fragments) is unexpected in view of the prior art described above.

[0121] Each of the improved LAMP constructs defined above can be made using the domains defined in the figures. For example, it is specifically contemplated that the domains included in the improved LAMP construct illustrated in FIG. 1 can originate from sequences derived from orthologous sequences. See, for example, FIGS. 3-10. It is specifically contemplated to use the equivalent domains defined in FIGS. 2A and 2B to make the improved LAMP construct illustrated in FIG. 1 for orthologous sequences. Furthermore, the orthologous sequences shown in FIGS. 3-10 are representative of sequences that can be used to generate the domains. It is well within the skill of the art to identify other orthologous sequences and / or isotypes and compare them to the alignments shown in FIGS. 3-10. Thus, the improved LAMP construct illustrated in FIG. 1 can be generated by identifying the equivalent boundaries defined in FIGS. 2A and 2B for the human LAMP protein having the sequence shown in FIGS. 3-10.

[0122] As will be appreciated by those of skill in the art, the boundaries of each domain are approximate and may be adjusted by at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 amino acids based on cloning considerations and restriction enzyme placement. Thus, when a particular domain (e.g., a LAMP homology domain) is included in an improved LAMP construct, the amino acids at the start and end of the domain may be adjusted by at least 1, 2, 3, 4, 5, 6, 7, 8, 9, or 10 amino acids in the boundaries defined in Figure 2B.

[0123] Each of the above improved LAMP constructs may further comprise a signal sequence and / or additional amino acids between each domain for cloning purposes, as is well known in the art. Furthermore, the LAMP homology domain, LAMP luminal domain, LAMP transmembrane domain, and / or LAMP cytosolic tail domain may originate from the same LAMP protein (e.g., human LAMP-1) or different LAMP proteins (e.g., the luminal domain from human LAMP-1, the transmembrane domain from human LAMP-2, and / or a mixture of orthologous domains from the same gene family (e.g., LAMP-1) or different gene families (LAMP-1 and LAMP-2)).

[0124] Polypeptide variants of the described LAMP constructs are contemplated. For example, polypeptides with at least about 60%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, 96%, 97%, 98% or 99% identity to any of the improved LAMP constructs described herein, as well as polynucleotides encoding these variants. The variants of the improved LAMP constructs retain the ability to function by targeting antigenic sequences to lysosomes. For example, the modified luminal sequence must retain the ability to transport both membrane and non-membrane antigenic materials to endosomal compartments with at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, or at least about 100% efficiency compared to the original domain sequence, i.e., efficiency that results in sufficient antigen presentation by cells containing the chimeric sequence to initiate an immune response. In one embodiment, sequences containing suitable transport signals can be identified by constructing improved LAMP constructs that contain the well-characterized antigenic domain of ovalbumin, the transmembrane domain, and the cytoplasmic domain of a protein that contains a putative lysosomal / endosomal targeting signal. Targeting efficiency can be measured by measuring the ability of antigen-presenting cells expressing the improved LAMP constructs to stimulate HA epitope-specific MHC class II restricted T cells (see, for example, Example 5 of U.S. Patent No. 5,633,234).

[0125] Polynucleotides encoding any of the described improved LAMP constructs are preferred embodiments of the present invention, as are polynucleotides with at least about 60%, at least about 70%, at least about 75%, at least about 80%, at least about 85%, at least about 90%, at least about 95%, 96%, 97%, 98% or 99% identity to any of the improved LAMP construct polynucleotides described herein. Variants of the improved LAMP constructs retain the ability to function by targeting antigenic sequences to lysosomes. For example, the modified luminal sequence must retain the ability to transport both membrane and non-membrane antigenic materials to endosomal compartments with at least about 50%, at least about 60%, at least about 70%, at least about 80%, at least about 90%, or at least about 100% efficiency compared to the original domain sequence, i.e., efficiency that results in sufficient antigen presentation by cells containing the chimeric sequence to initiate an immune response. In one embodiment, sequences containing suitable transport signals can be identified by constructing improved LAMP constructs that contain the well-characterized antigenic domain of ovalbumin, the transmembrane domain, and the cytoplasmic domain of a protein that contains a putative lysosomal / endosomal targeting signal. Targeting efficiency can be measured by measuring the ability of antigen-presenting cells expressing the improved LAMP constructs to stimulate HA epitope-specific MHC class II restricted T cells (see, for example, Example 5 of U.S. Patent No. 5,633,234).

[0126] Assembly of sequences encoding improved LAMP constructs Procedures for constructing improved LAMP constructs containing an antigen of interest are well known in the art (see, for example, Williams, et al., J. Cell Biol. 111:955, 1990). DNA sequences encoding the desired segments can be obtained from readily available recombinant DNA materials, such as those available from the American Type Culture Collection, 12301 Parklawn Drive, Rockville, Md. 20852, USA, or from DNA libraries containing the desired DNA.

[0127] For example, a DNA segment corresponding to a desired domain sequence can be assembled with appropriate control and signal sequences using conventional procedures of recombinant DNA methodology (see, e.g., U.S. Patent No. 4,593,002, and Langford, et al., Molec. Cell. Biol. 6:3191, 1986).

[0128] A DNA sequence encoding a protein or polypeptide can be chemically synthesized or isolated by one of several approaches. The DNA sequence to be synthesized can be designed with the appropriate codons for the amino acid sequence of interest. In general, codons that are preferred for the intended host in which the sequence will be used for expression are selected. The complete sequence can be assembled from overlapping oligonucleotides prepared by standard methods and assembled into a complete coding sequence. See, for example, Edge, Nature 292:756, 1981; Nambair, et al. Science 223:1299, 1984; Jay, et al., J. Biol. Chem. 259:6311, 1984.

[0129] In one embodiment, one or more nucleic acids encoding the domain sequences of the improved LAMP construct are individually isolated using the polymerase chain reaction (MAInnis, et al., In PCR Protocols: A Guide to Methods and Applications, Academic Press, 1990). The domains are preferably isolated from publicly available clones known to contain them, but may also be isolated from genomic DNA or cDNA libraries. Preferably, the isolated fragments are flanked by compatible restriction endonuclease sites that allow the construction of improved LAMP constructs encoding antigen sequences. This technique is well known to those skilled in the art. The domain sequences can be fused directly to each other (e.g., without intervening sequences) or inserted into each other (e.g., when the domain sequences are discontinuous) or separated by intervening sequences (e.g., linker sequences, etc.).

[0130] The basic strategies for preparing oligonucleotide primers, probes and DNA libraries, and their screening by nucleic acid hybridization, are well known to those skilled in the art. See, e.g., Sambrook, et al., 1989, supra; Perbal, 1984, supra. The construction of suitable genomic DNA or cDNA libraries is within the skill of the art. See, e.g., Perbal, 1984, supra. Alternatively, suitable DNA libraries or publicly available clones are available from suppliers of biological research materials, such as Clonetech and Stratagene, and from public depositories, such as the American Type Culture Collection.

[0131] Selection can be accomplished by expressing sequences from an expression library of DNA and immunologically detecting the expressed peptides. Clones expressing peptides that bind to MHC II molecules and the desired antibody / T cell receptor are selected. These selection procedures are well known to those skilled in the art (see, e.g., Sambrook, et al., 1989, supra).

[0132] Once a clone containing the coding sequence for the desired polypeptide sequence has been prepared or isolated, this sequence may be cloned into any suitable vector, preferably containing an origin of replication for maintaining the sequence in a host cell.

[0133] Nucleic acid delivery vehicle In one embodiment, the vaccine composition comprising the improved LAMP construct is introduced into a cell. The cell can be a host cell for replicating nucleic acid or for expressing the improved LAMP construct. Preferably, the host cell for expressing the improved LAMP construct is an antigen-presenting cell (as described further below).

[0134] In a preferred embodiment, the improved LAMP construct further comprises a polynucleotide sequence for insertion into a target cell and an expression control sequence operably linked thereto for controlling the expression (e.g., transcription and / or translation) of the polynucleotide sequence in the cell. Examples include plasmids, phages, autonomously replicating sequences (ARS), centromeres, and other sequences that are replicable or can be replicated in vitro or in a host cell (e.g., bacteria, yeast, insect cells, etc.) and / or in a target cell (e.g., mammalian cells, preferably antigen-presenting cells, etc.), and / or can deliver the sequence encoding the improved LAMP construct to a desired location in the target cell.

[0135] Recombinant expression vectors may be derived from microorganisms that readily infect animals such as humans, horses, cows, pigs, llamas, giraffes, dogs, cats, chickens, etc. Preferred vectors include those already used as live vaccines, such as vaccinia. These recombinants can be directly inoculated into the host, conferring immunity not only to the microbial vector, but also to the expression of the foreign antigen. Preferred vectors contemplated herein as live recombinant vaccines include RNA viruses, adenoviruses, herpes viruses, polio viruses, and vaccinia and other pox viruses, as taught, for example, in Flexner, Adv. Pharmacol. 21:51, 1990.

[0136] Expression control sequences include, but are not limited to, promoter sequences that bind RNA polymerase, enhancer sequences or negative regulatory elements that bind transcription activators and repressors, respectively, and / or translation initiation sequences that bind ribosomes.For example, bacterial expression vectors can include promoters such as lac promoters, and for transcription initiation, Shine-Dalgarno sequence and start codon AUG (Sambrook, et al., 1989, supra).Similarly, eukaryotic expression vectors preferably include heterologous, homologous, or chimeric promoters of RNA polymerase II, downstream polyadenylation signals, start codon AUG, and termination codons for ribosome detachment.

[0137] Expression control sequences can be obtained from naturally occurring genes or can be designed. Designed expression control sequences include, but are not limited to, mutated and / or chimeric expression control sequences or synthetic or cloned consensus sequences. Vectors that contain both a promoter and a cloning site into which a polynucleotide can be operably linked are well known in the art. Such vectors can transcribe RNA in vitro or in vivo and are commercially available from sources such as Stratagene (La Jolla, Calif.) and Promega Biotech (Madison, Wis.).

[0138] To optimize expression and / or transcription, it may be necessary to remove, add, or modify the 5' and / or 3' untranslated portions of the vector to eliminate extra or alternative translation initiation codons or other sequences that may interfere with or reduce expression at either the transcriptional or translational level. Alternatively, a consensus ribosome binding site can be inserted immediately 5' of the initiation codon to enhance expression. A wide variety of expression control sequences (sequences that control the expression of a DNA sequence operably linked to it) can be used in these vectors to express the DNA sequences of the present invention. Such useful expression control sequences include, for example, early or late promoters of SV40, CMV, vaccinia, polyoma, adenovirus, herpes virus, and other sequences known to control the expression of genes in mammalian cells, as well as various combinations thereof.

[0139] In one embodiment, the improved LAMP construct comprises an origin of replication for replicating the vector. Preferably, the origin functions in at least one type of host cell that can be used to generate a sufficient number of copies of the sequence for use in delivery to target cells. Thus, suitable origins include, but are not limited to, those that function in bacterial cells (e.g., Escherichia sp., Salmonella sp., Proteus sp., Clostridium sp., Klebsiella sp., Bacillus sp., Streptomyces sp., and Pseudomonas sp.), yeast (e.g., Saccharomyces sp. or Pichia sp., etc.), insect cells, and mammalian cells. In a preferred embodiment, an origin of replication is provided that functions in a target cell (e.g., a mammalian cell, such as a human cell) into which the nucleic acid delivery vehicle is introduced. In another embodiment, at least two origins of replication are provided, one that functions in the host cell and one that functions in the target cell.

[0140] Alternatively or additionally, the improved LAMP construct may comprise a sequence that promotes the integration of at least a portion of the nucleic acid delivery vector into the target cell chromosome. For example, the improved LAMP construct may comprise a region of homology with the target cell chromosomal DNA. In one embodiment, the delivery vector comprises two or more recombination sites adjacent to the nucleic acid sequence that codes for the improved LAMP construct.

[0141] Vectors may further include detectable and / or selectable markers to verify that the vector can be successfully introduced into and / or expressed by the target cell. These markers can code for an activity, such as, but not limited to, the production of RNA, peptides, or proteins, or can provide binding sites for RNA, peptides, proteins, inorganic and organic compounds or compositions, and the like.

[0142] Examples of detectable / selectable marker genes include, but are not limited to, the following: DNA segments that encode products that provide resistance to toxic compounds (e.g., antibiotics); DNA segments that encode products that are lacking in recipient cells (e.g., tRNA genes, auxotrophic markers); DNA segments that encode products that suppress the activity of a gene product; DNA segments that encode products that are easily identifiable (e.g., phenotypic markers such as beta-galactosidase, fluorescent proteins (GFP, CFP, YFG, BFP, RFP, EGFP, EYFP, EBFP, dsRed, mutant, modified or enhanced versions thereof, etc.), and cell surface proteins); DNA segments that bind products that are detrimental to cell survival and / or function; DNA segments that inhibit the activity of other nucleic acid segments (e.g., antisense oligonucleotides); DNA segments that bind products that modify substrates (e.g., restriction endonucleases); DNA segments that can be used to isolate or identify desired molecules (e.g., segments that encode specific protein binding sites); primer sequences; DNA segments that, if absent, directly or indirectly confer resistance or sensitivity to a particular compound; and / or DNA segments that encode products that are toxic to recipient cells.

[0143] The marker gene can be used as a marker of successful gene transfer conformation and / or to isolate cells expressing the transgene and / or to recover the transgene from the cells. For example, in one embodiment, the marker gene is used to isolate and purify antigen-presenting cells expressing the improved LAMP construct.

[0144] Substantially similar genes can be provided, for example, genes with identity of more than about 50%, more than about 70%, more than 80%, more than about 90%, preferably more than about 95% with known genes. Substantially similar domain sequences can be first identified by selecting sequences that specifically hybridize to the domain sequence of interest under stringent hybridization conditions. The execution of an assay to determine the suitability of homologous, mutated or modified domain sequences is simply screening for sequences that express appropriate activity. Such screening is routine in the art.

[0145] The improved LAMP constructs may be provided as naked nucleic acid or in a delivery vehicle associated with one or more molecules to facilitate entry of the nucleic acid into a cell. Suitable delivery vehicles include, but are not limited to, liposome formulations, polypeptides, polysaccharides, lipopolysaccharides, viral formulations (including, for example, viruses, viral particles, artificial viral envelopes, etc.), cell delivery vehicles, etc.

[0146] Lipid-Based Formulations A delivery vehicle designed to facilitate intracellular delivery of the improved LAMP construct must interact with both non-polar and polar environments (e.g., in plasma membranes, tissue fluids, intracellular compartments, etc.) Thus, preferably, the delivery vehicle is designed to include both polar and non-polar domains or translocation sequences for translocating the improved LAMP construct into cells.

[0147] Compounds with polar and nonpolar domains are called amphiphiles. Cationic amphiphiles have polar groups that can become positively charged at or near physiological pH in order to interact with negatively charged polynucleotides such as DNA.

[0148] The improved LAMP constructs described herein can be provided in a formulation that includes a lipid monolayer or bilayer to facilitate the translocation of vectors across cell membranes. Liposomes or any form of lipid membrane can be used, such as planar lipid membranes or intact cell membranes, such as red blood cells. The liposome formulations can be administered by any means, including intravenous or oral administration.

[0149] Liposomes and liposomal formulations can be prepared according to standard methods and are well known in the art, see, for example, Remington's; Akimaru, 1995, Cytokines Mol. Ther. 1:197-210; Alving, 1995, Immunol. Rev. 145:5-31; Szoka, 1980, Ann. Rev. Biophys. Bioeng 9:467; U.S. Patent No. 4,235,871; U.S. Patent No. 4,501,728; and U.S. Patent No. 4,837,028. In one embodiment, the liposome comprises a targeting molecule for targeting the liposome:improved LAMP construct complex to a specific cell type. In a particularly preferred embodiment, the targeting molecule comprises a binding partner (e.g., a ligand or receptor) for a biomolecule (e.g., a receptor or ligand) on the surface of blood vessels or cells found in the target tissue.

[0150] The charge of the liposome is an important determinant in the clearance of liposomes from the blood, with negatively charged liposomes being taken up more rapidly by the reticuloendothelial system (Juliano, 1975, Biochem. Biophys. Res. Commun. 63:651), and therefore having a shorter half-life in the bloodstream. Incorporation of phosphatidylethanolamine derivatives extends circulation time by preventing liposome aggregation. For example, incorporation of N-(omega-carboxy)acylamidophosphatidylethanolamine into large unilamellar vesicles of L-alpha-distearoylphosphatidylcholine dramatically extends liposome circulation lifetime in vivo (see, for example, Ahl, 1997, Biochim. Biophys. Acta 1329:370-382). Liposomes with extended circulation half-lives are usually desirable for therapeutic and diagnostic applications. For a general discussion of pharmacokinetics, see, e.g., Emington's, Chapters 37-39, Lee, et al., In Pharmacokinetic Analysis: A Practical Approach (Technomic Publishing AG, Basel, Switzerland 1996).

[0151] Typically, liposomes are prepared with about 5-15 mole percent of negatively charged phospholipids such as phosphatidylglycerol, phosphatidylserine or phosphatidylinositol. The addition of negatively charged phospholipids such as phosphatidylglycerol also serves to prevent spontaneous liposome aggregation, thus minimizing the risk of under-forming liposome aggregates. A concentration of at least about 50 mole percent of sphingomyelin or saturated neutral phospholipids and 5-15 mole percent of a membrane stiffening agent such as monosialyl ganglioside may also impart desirable liposome properties such as stiffness (see, for example, U.S. Pat. No. 4,837,028).

[0152] Additionally, the liposomal suspension may contain lipid-protective agents that protect lipids from free radical and lipid peroxidative damage upon storage. Lipophilic free-radical quenchers such as α-tocopherol and water-soluble iron-specific chelators such as ferrioxianine are preferred.

[0153] The improved LAMP constructs of the present invention can include multilamellar vesicles of heterogeneous size. For example, the vesicle-forming lipids can be dissolved in a suitable organic solvent or solvent system and dried under vacuum or inert gas to form a thin lipid film. If necessary, the film can be redissolved in a suitable solvent such as tertiary butanol and lyophilized to form a more homogenous lipid mixture in a powder form that is more easily hydrated. This film can be covered with an aqueous solution of the peptide or polypeptide complex and allowed to hydrate, typically for 15-60 minutes with stirring. The size distribution of the resulting multilamellar vesicles can be shifted to smaller sizes by hydrating the lipids under more vigorous stirring conditions or by adding a solubilizing detergent such as deoxycholic acid. The hydration medium preferably contains the desired concentration of nucleic acid in the interior volume of the liposomes in the final liposome suspension.

[0154] After liposome preparation, the liposomes can be sized to achieve a desired size range and a relatively narrow distribution of liposome sizes. One preferred size range is about 0.2-0.4 microns, which allows the liposome suspension to be sterilized by filtration through a conventional filter, typically a 0.22 micron filter. If the liposomes are reduced in size to about 0.2-0.4 microns, filter sterilization can be performed at high throughput. Several techniques are available for sizing liposomes to the desired size (see, e.g., U.S. Pat. No. 4,737,323).

[0155] Suitable lipids include, but are not limited to, DOTMA (Felgner, et al., 1987, Proc. Natl. Acad. Sci. USA 84:7413-7417), DOGS or Transfectain™ (Behr, et al., 1989, Proc. Natl. Acad. Sci. USA 86:6982-6986), DNERIE or DORIE (Felgner, et al., Methods 5:67-75), DC-CHOL (Gao and Huang, 1991, BBRC 179:280-285), DOTAP™ (McLachlan, et al., 1995, Gene Therapy 11:131-132, 1997). 2:674-622), Lipofectamine™ and glycerolipid compounds (see, e.g., EP 901463 and WO 98 / 37916).

[0156] Other molecules suitable for conjugation to the improved LAMP constructs include cationic molecules such as polyamidoamines (Haensler and Szoka, 1993, Bioconjugate Chem. 4:372-379), dendritic polysine (WO 95 / 24221), polyethylene rinine or polypropylene h-nine (WO 96 / 02655), polylysine (U.S. Pat. No. 5,595,897; French Patent No. 2719316), chitosan (U.S. Pat. No. 5,744,166), DNA-gelatin coacervates (e.g., U.S. Pat. No. 6,207,195; U.S. Pat. No. 6,025,337; U.S. Pat. No. 5,972,707) or DEAE dextran (Lopata, et al., 1984, Nucleic Acid Res. 12:5707-5717).

[0157] Viral-Based Gene Delivery Vehicles In one embodiment, the improved LAMP construct delivery vehicle comprises a virus or a viral particle. In this embodiment, preferably, the improved LAMP construct comprises a viral vector. Viral vectors, such as retroviruses, adenoviruses, adeno-associated viruses, and herpes viruses, are often composed of two components, a modified viral genome and a coat structure surrounding it (see, for example, Smith et al., 1995, Ann. Rev. Microbiol. 49:807-838), but viral vectors can be introduced in a naked form or coated with proteins other than viral proteins. Most current vectors have coat structures similar to wild-type viruses. This structure packages and protects viral nucleic acid and provides a means for binding and entering target cells.

[0158] Preferably, the viral vectors comprising the improved LAMP constructs described herein are modified from the wild-type viral genome so that the virus cannot grow in the target cell but can grow in the host cell (e.g., packaging or helper cell) used to prepare the infectious particle. The vector nucleic acid generally requires cis-acting viral sequences for replication and packaging in the helper system, and expression control sequences for regulating the expression of the polynucleotide delivered to the target cell. Other viral functions are expressed in trans in a particular packaging or helper cell line, as known in the art.

[0159] A preferred improved LAMP construct is a viral vector derived from a virus selected from the group consisting of herpesvirus, cytomegalovirus, foamy virus, lentivirus, Semliki Forest virus, AAV (adeno-associated virus), poxvirus, adenovirus and retrovirus. Such viral vectors are well known in the art.

[0160] In a preferred embodiment, the viral vector used is an adenoviral vector. The adenoviral genome is composed of a linear double-stranded DNA molecule of about 36 kb carrying more than about 30 genes necessary to complete the viral replication cycle. The early genes are divided into four regions (E1-E4), which are essential for viral replication, apart from the E3 region, which is thought to regulate the antiviral host immune response. The E1 region (EIA and EIB) encodes proteins involved in the transcriptional control of the viral genome. Expression of the E2 region genes (E2A and E2B) results in the synthesis of polypeptides necessary for viral replication. The proteins encoded by the E3 region prevent cell lysis by cytotoxic T cells and tumor necrosis factor (Wold and Gooding, 1991, Virology 184:1-8). The proteins encoded by the E4 region are involved in DNA replication, late gene expression and splicing, and shutting off the host cell (Halbert, et al., 1985, J. Virol. 56:250-257). Late genes generally code for structural proteins that contribute to the viral capsid. In addition, the adenovirus genome possesses cis-acting 5' and 3' ITRs (inverted terminal repeats) and packaging sequences essential for DNA replication. The ITRs contain the origin of DNA replication, and the encapsidation region is required for packaging of adenoviral DNA into infectious particles.

[0161] As described by Heise and Kim (2000, J. Clin. Invest. 105:847-85 1 , adenoviral vectors can be engineered to be conditionally replicating (CRAd vectors) in order to selectively replicate in certain cells (e.g., proliferating cells, etc.). In another embodiment, the adenoviral vector is replication-deficient for E1 function (e.g., by total or partial deletion or mutagenesis of E1). The adenoviral backbone of the vector may contain additional modifications (deletions, insertions or mutations of one or more viral genes). An example of an E2 modification is shown by a temperature-sensitive mutation located in the gene encoding DBP (DNA Binding Protein) (Ensinger et al., 1972, J. Virol. 10:328-339). The adenoviral sequence may be deleted of all or part of the E4 region (see, e.g., European Patent No. 974668; Christ, et al., 2000, Human Gene Ther. 11:415-427; Lusky, et al., 1999, J. Virol. 73:8308-8319). Additional deletions within the non-essential E3 region can increase the size of the polynucleotide delivered (Yeh, et al., 1997, FASEB Journal 11:615 623). However, it may be advantageous to retain all or part of the E3 sequence that encodes a polypeptide (such as gp19k) that allows the virus to escape the immune system (Gooding, et al., 1990, Critical Review of Immunology 10:53-71) or inflammatory response (European Patent No. 00440267.3).

[0162] Second generation vectors that retain the ITRs and packaging sequences and contain substantial genetic modifications that abolish residual synthesis of viral antigens can also be used to improve long-term expression of expressed genes in transduced cells (see, e.g., WO 94 / 28152; Lusky, et al., 1998, J. Virol 72:2022-2032).

[0163] The improved LAMP construct introduced into the cell can be inserted anywhere in the viral genome, except for the cis-acting sequences. Preferably, it is inserted in place of the deleted region (E1, E3 and / or E4), preferably within the deleted E1 region.

[0164] The adenovirus may be from any human or animal source, in particular canine (e.g. CAV-1 or CAV-2, Genbank refs. CAVIGENOM and CAV77082, respectively), avian (Genbank ref. AAVEDSDNA), bovine (e.g. BAV3, Reddy, et al., 1998, J. Virol. 72:1394 1402), murine (Genbank ref. ADRMUSMAVI), ovine, feline, porcine or simian origin, or may be a hybrid virus. Any serotype may be used. However, human adenoviruses of the C subgroup are preferred, in particular adenovirus 2 (Ad2) and 5 (Ad5). Such viruses are available, for example, from the ATCC.

[0165] Adenoviral particles or empty adenoviral capsids can also be used to introduce the improved LAMP constructs by the virus-mediated co-internalization method described in U.S. Patent No. 5,928,944. This method can be accomplished in the presence of cationic agent(s), such as polycarbene or lipid vesicles containing one or more lipid layers.

[0166] Adenoviral particles can be prepared and propagated according to any conventional technique in the art (e.g., WO 96 / 17070) by using complementation cell line or helper virus that provides the defective viral gene required for viral replication in trans.Cell line 293 (Graham et al., 1977, J.Gen.Virol.36:59-72) and PERC6 (Fallaux et al., 1998, Human Gene Therapy 9:1909-1917) are commonly used to complement E1 deletion. Other cell lines have been engineered to complement defective vectors (Yeh, et al., 1996, J. Virol. 70:559-565; Kroughak and Graham, 1995, Human Gene Ther. 6:1575-1586; Wang, et al., 1995, Gene Ther. 2:775-783; Lusky, et al., 1998, J. Virol. 72:2022-203; EP 919627 and WO 97 / 04119). Adenoviral particles can be recovered from culture supernatants or from cells after lysis, and can be optionally further purified according to standard techniques (e.g., chromatography, ultracentrifugation, as described in WO 96 / 27677, WO 98 / 00524, WO 98 / 26048 and WO 00 / 50573).

[0167] Cell type specific targeting can be achieved with vectors derived from adenoviruses with a broad host range by modifying viral surface proteins.For example, the specificity of adenovirus infection is determined by attachment to cell receptors present on the surface of permissive cells.In this regard, the fiber and penton present on the surface of adenovirus capsid play an important role in cell attachment (Defer, et al., 1990, J.Virol.64:3661-3673).Therefore, adenovirus cell targeting can be carried out by genetically modifying the viral genes that code for fiber and / or penton to generate modified fiber and / or penton that can specifically interact with unique cell surface receptors. Examples of such modifications are described in Wickarn, et al., 1997, J. Virol. 71:8221-8229; Arriberg, et al., 1997, Virol. Chem 268:6866-6869; Roux, et al., 1989, Proc. Natl. Acad. Sci. USA 86:9079-9083; Miller and Vile, 1995, FASEB J. 9:190-199; WO 93 / 09221 and WO 95 / 28494.

[0168] In a particularly preferred embodiment, adeno-associated virus sequences are used as vectors. Vectors derived from the human parvovirus AAV-2 (adeno-associated virus type 2) are one of the most promising gene delivery vehicles currently being developed. Some of the features of this system for packaging single-stranded DNA suggest that it could be an alternative to naked DNA for delivery. In contrast to other viral vectors such as vaccinia or adenovirus, the main attractive feature is that AAV vectors do not express any viral genes. The only viral DNA sequence contained in the vaccine construct is a 145 bp inverted terminal repeat (ITR). Thus, as in the case of naked DNA immunization, the only gene expressed is that of the antigen or antigen chimera. Furthermore, AAV vectors are known to introduce persistent expression of transgenes into both dividing and non-dividing cells, such as human peripheral blood monocyte-derived dendritic cells, and the possibility of oral and intranasal delivery for the generation of mucosal immunity. Furthermore, the amount of DNA required appears to be several orders of magnitude less, with naked DNA doses of 50ug or ca. 15 10 is a copy 10 ~10 11 The dose of particles or DNA copies gives the maximal response.

[0169] In one embodiment, the AAV vector is packaged in a suitable cell line (e.g., human 293 cells) by co-transfection of DNA contained in the AAV ITR chimeric protein encoding construct with the AAV helper plasmid ACG2, which contains the AAV coding region without the ITRs (AAV rep and cap genes). The cells are then infected with adenovirus Ad5. The vector can be purified from cell lysates using methods known in the art (e.g., cesium chloride density gradient ultracentrifugation, etc.) and verified (e.g., by cytopathic effect bioassay) to ensure that it does not contain detectable replication-competent AAV or adenovirus. The AAV titer can be determined by quantitative PCR using viral DNA samples prepared after digestion with proteinase K. Preferably, the vector titer purified by such methods is about 5x10 12 ~1x10 13 DNase resistant particles / ml.

[0170] In other embodiments, retroviral vectors are used. Retroviruses are a class of integrative viruses that replicate using a virally encoded reverse transcriptase enzyme to replicate the viral RNA genome into double-stranded DNA that is integrated into the chromosomal DNA of infected cells (e.g., target cells). Such vectors include those derived from murine leukemia viruses, particularly Moloney (Gilboa, et al., 1988, Adv. Exp. Med. Biol. 241:29) or Friend's FB29 strain (WO 95 / 01447). In general, retroviral vectors are deleted of all or part of the viral genes gag, pol and env, and retain the 5' and 3' LTRs and encapsidation sequences. These elements can be modified to increase the expression level or stability of the retroviral vector. Such modifications include the replacement of the retroviral encapsidation sequence with one of the retrotransposons, such as VL30 (see, e.g., U.S. Pat. No. 5,747,323). Preferably, the improved LAMP construct is inserted downstream of the encapsidation sequence, preferably in the opposite orientation to the retroviral genome. Cell-specific targeting can be achieved by conjugation of antibodies or antibody fragments to retroviral envelope proteins, as known in the art.

[0171] Retroviral particles are prepared in the presence of helper virus or in suitable complementing (packaging) cell lines in which retroviral genes (e.g., gag / pol and env) that are missing from the retroviral vector are integrated into the genome. Such cell lines are described in the prior art (Miller and Rosman, 1989, BioTechniques 7:980; Danos and Mulligan, 1988, Proc. Natl. Acad. Sci. USA 85:6460; Markowitz, et al., 1988, Virol. 167:400). The product of the env gene is responsible for binding of the virus particle to the viral receptor present on the surface of the target cell, thus determining the host range of the retroviral particle. In the context of the present invention, it is advantageous to use packaging cell lines that contain amphotropic envelope proteins, such as PA317 cells (ATCC CRL 9078) or 293EI6 (WO97 / 35996), to allow infection of human and other species of target cells. Retroviral particles are preferably recovered from the culture supernatant and may optionally be further purified according to standard techniques (eg, chromatography, ultracentrifugation).

[0172] Other suitable viruses include poxviruses. The genomes of several members of the poxyiridae family have been mapped and sequenced. Poxvirus vectors can be derived from any member of the poxyiridae family, in particular canarypox virus, fowlpox virus and vaccinia virus. Suitable vaccinia viruses include, but are not limited to, the Copenhagen strain (Goebel, et al., 1990, Virol. 179:247-266; Johnson, et al., 1993, Virol. 196:381-401), the Wyeth strain and the modified Ankara (MVA) strain (Antoine, et al., 1998, Virol. 244:365-396). General conditions for constructing vaccinia virus vectors are known in the art (see, for example, EP 83286 and EP 206920; Mayr et al., 1975, Infection 3:6-14; Sutter and Moss, 1992, Proc. Natl. Acad. Sci. USA 89:10847-10851).Preferably, the polynucleotide of interest is inserted into a non-essential locus, such as a non-coding intergenic region or any gene whose inactivation or deletion does not significantly impair viral growth and replication.

[0173] Poxyiral particles are prepared as described in the art (Piccini, et al., 1987, Methods of Enzymology 153:545-563; U.S. Patent No. 4,769,330; U.S. Patent No. 4,772,848; U.S. Patent No. 4,603,112; U.S. Patent No. 5,100,587 and U.S. Patent No. 5,179,993). In general, a donor plasmid is constructed, amplified by growth in E. coli, and isolated by conventional procedures. It is then introduced into a suitable cell culture (e.g., chicken embryo fibroblasts) along with the poxvirus genome, which generates poxyiral particles by homologous recombination. These can be recovered from the culture supernatant or from the cultured cells after a lysis step (e.g., chemical lysis, freeze / thaw, osmotic shock, sonication, etc.). Successive plaque purifications can be used to remove contaminating wild-type virus. The viral particles can then be purified using techniques known in the art (eg, chromatographic methods or ultracentrifugation on cesium chloride or sucrose gradients).

[0174] The use of vaccinia as a live viral vaccine in the worldwide campaign for smallpox eradication made it the clear choice for development as a live recombinant vaccine vector. Live recombinant vaccinia viruses expressing nearly 100 different foreign proteins have been reported, many of which are effective experimental vaccines (reviewed by Moss and Flexner, 1987). Vaccinia is particularly versatile as an expression vector because of its large genome size, its ability to accept foreign DNA of at least 25,000 base pairs, and its ability to infect most eukaryotic cell types, including insect cells (ibid.). Unlike other DNA viruses, poxviruses replicate exclusively in the cytoplasm of infected cells, reducing the possibility of genetic exchange of recombinant viral DNA with the host chromosome. Recombinant vaccinia vectors have been shown to properly process and express proteins from a variety of sources, including humans, other mammals, parasites, RNA and DNA viruses, bacteria and bacteriophages.

[0175] Expression of DNA encoding a foreign protein is controlled by host virus regulatory elements, such as upstream promoter sequences and, if necessary, RNA processing signals. Insertion of foreign DNA into non-essential regions of the vaccinia virus genome has been achieved by homologous recombination (Panicali, et al., Proc. Nat'l. Acad. Sci, USA, 79:4927, 1982; Mackett, et al., Proc. Nat'l. Acad. Sci, USA, 79:7415, 1982).

[0176] Expression of antigens by improved LAMP constructs can be achieved by transcriptional regulatory elements at or near the insertion site, or by more precise genetic engineering. Plasmid vectors have been constructed that greatly facilitate the insertion and expression of foreign genes (Mackett, et al., J. Virol, 49:857, 1982). These vectors contain an expression site consisting of a vaccinia transcriptional promoter and one or more unique restriction endonuclease sites for the insertion of foreign coding sequences, flanked by DNA from non-essential regions of the vaccinia genome. The choice of promoter determines both the time of expression (e.g., early or late) and the level of expression, while the flanking DNA sequences determine the site of homologous recombination.

[0177] Of the virus particles generated by this procedure, only about one in 1000 are recombinant. Recombinant virus plaques can be identified by DNA hybridization, but efficient selection procedures have been developed. Foreign genes are recombined into the TK locus by using segments of the non-essential vaccinia virus thymidine kinase (TK) gene as flanking sequences, inactivating the TK gene by insertion. Selection of TK viruses is achieved by performing a virus plaque assay in TK cells in the presence of 5-bromodeoxyuridine. Phosphorylation of the nucleoside analogue and the resulting lethal integration into viral DNA occurs only in cells infected with the TK+ parent virus. Depending on the efficiency of transfection and recombination, up to 80 plaques are the desired recombinants, while the rest are spontaneous TK mutants.

[0178] Plasmid vectors containing the E. coli beta-galactosidase gene and an expression site for a second gene allow an alternative method of distinguishing recombinant viruses from the parent virus (Chakrabarti, et al., Mol. Cell. Biol., 5:3403, 1985). Plaques formed by such recombinants can be clearly identified by the blue color they form upon addition of an appropriate indicator. By combining both TK selection and beta-galactosidase expression, recombinant viruses are easily and quickly isolated. Recombinants are then amplified by growth in an appropriate cell line, and expression of the inserted gene is confirmed by appropriate enzymatic, immunological or physical procedures.

[0179] The upper limit to the amount of genetic information that can be added to the vaccinia virus genome is unknown; however, the addition of approximately 25,000 base pairs of foreign DNA had no apparent adverse effect on virus yield (Smith, et al., Gene, 25:21, 1983). Large segments of the vaccinia virus genome can be deleted to provide additional capacity, if necessary (Moss, et al., J. Virol. 40:387, 1981).

[0180] The viral capsid molecule may include a targeting moiety that facilitates targeting and / or cell entry. Suitable targeting molecules include, but are not limited to, chemical conjugates, lipids, glycolipids, hormones, sugars, polymers (e.g., PEG, polylysine, PEI, etc.), peptides, polypeptides (see, e.g., WO 94 / 40958), vitamins, antigens, lectins, antibodies and fragments thereof. Preferably, such targeting molecules recognize and bind to cell-specific markers, tissue-specific markers, cell receptors, viral antigens, antigenic epitopes, or tumor-associated markers.

[0181] The composition containing the improved LAMP construct based on viral particles is 10-10 14 iu (infectious unit), preferably 10 to 10 11 iu doses. The potency can be determined by conventional techniques. The dose of the LAMP construct is preferably comprised between 0.01 and 10 mg / kg, more particularly between 0.1 and 2 mg / kg.

[0182] self-replicating RNA Self-replicating RNA virus vectors can also be constructed using the improved LAMP constructs described herein.For example, alphavirus, flavivirus, measle virus and rhabdovirus can be used to generate self-replicating RNA virus vaccines.Preferred strains of self-replicating RNA virus include, but are not limited to, rabies virus (RABV), vesicular stomatitis virus (VSV), West Nile virus, Kunjin virus, Semliki Forest virus (SFV), Sindbis virus (SIN) and / or Venezuelan equine encephalitis virus (VEE).

[0183] Self-replicating RNA viruses express native antigens upon delivery to tissues, thus mimicking live attenuated vaccines without the risk of reversion to pathogenicity. They also stimulate the innate immune system and enhance responses. See, for example, Ljungberg, K. “Self-replicating alphavirus RNA vaccines,” Expert Rev Vaccines(2):177-94(2015); Lundstrom, K., “Oncolytic Alphaviruses in Cancer Immunotherapy,” Vaccines 5:9(2017); Lundstrom, K. “Replicon RNA Viral Vectors as Vaccines,” Vaccines 4:39(2016), which are incorporated herein by reference in their entirety. The use of self-replicating vaccines, including the improved LAMP constructs described herein, can also be used in prime-boost protocols.

[0184] Furthermore, as described herein, self-replicating RNA viruses can be encapsulated in liposomes to improve delivery and targeting. Immunization with self-replicating RNA viruses, including the improved LAMP constructs described herein, provides higher transient expression levels of antigens, resulting in the generation of neutralizing antibody responses and protection against lethal challenge under safe conditions.

[0185] Cell-Based Delivery Vehicles The improved LAMP construct of the present invention can be delivered to a target cell by another cell ("delivery cell") that contains the construct. Methods for introducing the construct into a cell are known in the art and include microinjection of DNA into the nucleus of the cell (Capechi, et al., 1980, Cell 22:479-488); 4(Chen and Okayama, 1987, Mol. Cell Biol. 7:2745 2752), electroporation (Chu, et al., 1987, Nucleic Acid Res. 15:1311-1326); lipofection / liposome fusion (Feigner, et al., 1987, Proc. Natl. Acad. Sci. USA 84:7413-7417) and particle bombardment (Yang, et al., 1990, Proc. Natl. Acad. Sci. USA 87:9568-9572). Suitable cells include autologous and non-autologous cells, and may include heterologous cells. Delivery cells can be induced to deliver their contents to target cells by inducing their death (e.g., by providing these cells with an inducible suicide gene).

[0186] Accessory molecules Compositions comprising the improved LAMP constructs of the present invention may contain one or more accessory molecules to facilitate the introduction of the improved LAMP construct into cells and / or to enhance a particular therapeutic effect and / or to enhance antibody production.

[0187] Additionally, compositions comprising the improved LAMP constructs according to the present invention may contain one or more stabilizing substances, e.g., lipids, nuclease inhibitors, hydrogels, hyaluronidase (WO 98 / 53853), collagenase, polymers, chelating agents (EP 890362), to inhibit degradation in the animal / human body and / or to improve transfection / infection of the vector into target cells. Such substances may be used alone or in combination (e.g., cationic and neutral lipids).

[0188] It has also been shown that adenovirus proteins destabilize endosomes and enhance DNA uptake into cells. Mixing adenovirus into a solution containing lipid-complexed DNA vectors, or binding DNA to polylysine covalently linked to adenovirus using a protein cross-linker, can greatly improve uptake and expression of modified LAMP constructs (e.g., Curiel, et al., 1992, Am. I. Respir. Cell. Mol. Biol. 6:247-252).

[0189] host cell The improved LAMP constructs according to the present invention can be expressed in a variety of host cells, including but not limited to prokaryotic cells (e.g., E. coli, Staphylococcus sp., Bacillus sp.); yeast cells (e.g., Saccharomyces sp.); insect cells; nematode cells; plant cells; amphibian cells (such as Xenopus laevis); avian cells; and mammalian cells (e.g., human cells, mouse cells, mammalian cell lines, primary cultured mammalian cells from dissected tissues, etc.).

[0190] The molecule can be expressed in a host cell isolated from an organism, a host cell that is part of an organism, or a host cell introduced into an organism. In one embodiment, the improved LAMP construct is expressed in a host cell in vitro, for example in culture. In another embodiment, the improved LAMP construct is expressed in a transgenic organism (e.g., a transgenic mouse, rat, rabbit, pig, primate, etc.) that contains somatic and / or germline cells that contain a nucleic acid encoding the improved LAMP construct. Methods for constructing transgenic animals are well known and routine in the art.

[0191] The improved LAMP constructs can be introduced into cells in vitro, or cells (e.g., stem cells, hematopoietic cells, lymphocytes, etc.) can be introduced into a host organism. The cells can be xenogeneic or autologous with respect to the host organism. For example, cells can be obtained from a host organism and the improved LAMP constructs can be introduced into the cells in vitro and then reintroduced into the host organism.

[0192] antigen presenting cells In a preferred embodiment of the present invention, the improved LAMP constructs described herein are introduced into natural or engineered antigen-presenting cells.

[0193] The term "antigen presenting cell" (APC) as used herein refers to any cell that presents an antigen associated with a major histocompatibility complex molecule, preferably a class II molecule or a part thereof, on its surface. Examples of suitable APCs are described in detail below, but include, but are not limited to, whole cells such as macrophages, dendritic cells, B cells, hybrid APCs, and foster antigen presenting cells. Methods for making hybrid APCs have been described and are known in the art.

[0194] Dendritic cells (DCs) are potent antigen-presenting cells. DCs have been shown to provide all the signals necessary for T cell activation and proliferation. These signals can be classified into two types. The first type, which confers specificity to the immune response, is mediated by the interaction of the T cell receptor / CD3 ("TCR / CD3") complex with antigenic peptides presented by major histocompatibility complex ("MHC") class I or II proteins on the surface of APCs. This interaction is necessary but not sufficient for T cell activation to occur. In fact, in the absence of the second type of signal, the first type of signal can result in T cell immune unresponsiveness. The second type of signal, called costimulatory signals, is neither antigen-specific nor MHC-restricted and can result in a complete T cell proliferative response and induction of T cell effector functions in the presence of the first type of signal.

[0195] Several molecules have been shown to enhance costimulatory activity, including, but not limited to, heat stable antigen (HSA), chondroitin sulfate-modified MHC invariant chain (Ii-CS), intracellular adhesion molecule I (ICAM-1), and the B7 costimulatory molecule on the surface of APCs, and its counter-receptors CD28 or CTLA-4 on T cells.

[0196] Other important costimulatory molecules are CD40, CD54, CD80, CD86. The term "costimulatory molecule" as used herein includes any single molecule or combination of molecules that, when acting together with the peptide / MHC complex bound by the TCR on the surface of the T cell, provide a costimulatory effect that achieves activation of the T cell that binds to the peptide. Thus, the term includes B7, or other costimulatory molecules on the APC, fragments thereof (alone, complexed with another molecule(s) or as part of a fusion protein) that, when the TCR on the surface of the T cell specifically binds to the peptide, binds to the cognate ligand together with the peptide / MHC complex, resulting in activation of the T cell. Costimulatory molecules are commercially available from a variety of sources, including, for example, Beckman Coulter.

[0197] In one embodiment of the present invention, the method described in Romani et al., J.Immunol.Methods 196:135-151, 1996, and Bender et al., J.Immunol.Methods 196:121-135, 1996 is used to generate both immature and mature dendritic cells from peripheral blood mononuclear cells (PBMCs) of mammals such as mice, monkeys, and humans. Briefly, isolated PBMCs are pre-treated to deplete T cells and B cells by immunomagnetic techniques. The lymphocyte-depleted PBMCs are then cultured (e.g., for about 7 days) in RPMI medium 9 supplemented with human plasma (preferably autologous plasma) and GM-CSF / IL-4 to generate dendritic cells. Dendritic cells are non-adherent compared to monocyte precursor cells. Thus, on about day 7, non-adherent cells are harvested for further processing.

[0198] Dendritic cells derived from PBMC in the presence of GM-CSF and IL-4 are immature in that they lose their non-adhesiveness and can revert to a macrophage cell fate when cytokine stimulation is removed from the culture. Dendritic cells in the immature state are very efficient at processing natural protein antigens in the MHC class II restricted pathway (Romani, et al., J. Exp. Med. 169:1169, 1989). Further maturation of cultured dendritic cells is achieved by culturing for 3 days in macrophage conditioned medium (CM) containing the necessary maturation factors. Mature dendritic cells are less capable of capturing new proteins for presentation, but are much better at stimulating resting T cells (both CD4 and CD8) to grow and differentiate.

[0199] Mature dendritic cells can be identified by their morphological changes, such as the formation of more motile cytoplasmic processes, by their non-adhesiveness, by the presence of at least one of the following markers: CD83, CD68, HLA-DR or CD86; or by the loss of Fc receptors, such as CD 115 (reviewed in Steinman, Annu. Rev. Immunol. 9:271, 1991). Mature dendritic cells can be collected and analyzed using typical cytofluorometry and cell sorting techniques and devices, such as FACScan and FACStar. Primary antibodies used for flow cytometry are specific for cell surface antigens of mature dendritic cells and are commercially available. Secondary antibodies can be biotinylated Ig followed by FITC or PE-conjugated streptavidin.

[0200] Alternatively, methods have been reported to upregulate (activate) dendritic cells and convert monocytes to an activated dendritic cell phenotype. In this method, monocytes are converted to activated dendritic cells by the addition of calcium ionophore to the culture medium. For example, the addition of calcium 21 ionophore A23187 at the beginning of the 24-48 h culture period results in uniform activation and dendritic cell phenotype conversion of the pooled "monocyte+DC" fraction; characteristically, the activated population is uniformly CD14(Leu M3) negative and upregulates HLA-DR, HLA-DQ, ICAM-1, 137.1, and 137.2. Moreover, this activated bulk population functions similarly on a minority basis when further purified. Specific (one or more) combinations of cytokines have been successfully used to amplify (or partially replace) the activation / conversion achieved with calcium ionophore; these cytokines include, but are not limited to, G-CSF, GM-CSF, IL-2, and IL-4. When administered alone, each cytokine is insufficient for optimal upregulation.

[0201] The second approach to isolate APCs is to collect the relatively large number of pre-committed APCs already circulating in the blood. Previous techniques for isolating committed APCs from human peripheral blood included physical procedures such as metrizamide gradients in combination with adherent / non-adherent steps (Freudenthal et al. PNAS 87:7698-7702, 1990); Percoll gradient separation (Mehta-Damani, et al., J.Immunol.153:996-1003, 1994); and fluorescence-activated cell sorting techniques (Thomas et al., J.Immunol.151:6840-52, 1993).

[0202] There are many other methods routine in the art for isolating professional antigen-presenting cells (or their precursors), and such methods and others that may be developed are not limiting and are encompassed within the scope of the present invention.

[0203] In one embodiment, the APCs, and thus the cells presenting one or more antigens, are autologous, hi another embodiment, the antigen-presenting APCs are allogeneic, i.e., derived from a different subject.

[0204] As discussed herein, the improved LAMP constructs can be introduced into APCs using the above methods or other methods known in the art, including but not limited to transfection, electroporation, fusion, microinjection, viral-based delivery, or cell-based delivery. Arthur et al., Cancer Gene Therapy 4(1):17-25, 1997, reports a comparison of gene transfer methods in human dendritic cells.

[0205] The known partial and deduced amino acid and nucleotide sequences, including consensus sequences, of human leukocyte antigens (HLA), the genetic designation for human MHC, have been published (see, e.g., Zemmour and Parham, Immunogenetics 33:310-320, 1991), and cell lines expressing HLA variants are known, many of which are publicly available from the American Type Culture Collection ("ATCC"). Thus, using PCR, a nucleotide sequence encoding MHC class II is readily operably linked to an expression vector of the invention and then used therein to transform appropriate cells for expression.

[0206] Professional APCs such as macrophages, B cells, monocytes, dendritic cells, Langerhans cells, etc. can be used. They are collected from blood or tissue from 1) autologous donors; 2) xenogeneic donors with different HLA specificities than the host being treated; or 3) xenogeneic donors of different species using standard procedures (Coligan, et.al., Current Protocols in Immunology, sections 3 and 14, 1994). Cells may be isolated from normal hosts or from patients with infections, cancer, autoimmune diseases, or allergies.

[0207] Professional APCs can be obtained from peripheral blood using leukodepletion and "FICOLL / HYPAQUE" density gradient centrifugation (stepwise centrifugation through Ficoll and discontinuous Percoll density gradients), a procedure that avoids exposure of APCs to antigens that may be internalized by the APC, resulting in activation of T cells that are not specific for the antigen of interest.

[0208] Cells that are not naturally antigen-presenting can be engineered to become antigen-presenting by introducing sequences encoding the appropriate molecules. For example, nucleic acid sequences encoding MHC class II molecules, accessory molecules, costimulatory molecules, and antigen processing auxiliary molecules can be introduced following direct synthesis, cloning, purification, etc. of DNA from cells containing such genes. One convenient means for obtaining genes encoding molecules used in the improved LAMP constructs and methods described herein is by polymerase chain reaction (PCR) amplification using selected oligonucleotide primer pairs on selected nucleic acid templates. For example, epithelial cells, endothelial cells, tumor cells, fibroblasts, activated T cells, eosinophils, keratinocytes, astrocytes, microglial cells, thymic cortical epithelial cells, Schwann cells, retinal pigment epithelial cells, myoblasts, vascular smooth muscle cells, chondrocytes, intestinal cells, thyroid cells, and renal tubule cells can be used. These may be primary cells that have been recently explanted from the host and have not been passaged extensively in cell culture to form an established cell line, or they may be established cell lines that are relatively homogenous and can grow indefinitely for many generations.

[0209] Cells that are not professional APCs can be isolated from any tissue of an autologous, xenogeneic or xenogeneic donor where they reside using a variety of known isolation methods (Darling, Animal Cells: Culture and Media. J. Wiley, New York, 1994; Freshney, Culture of Animal Cells. Alan R. Liss, Inc., New York, 1987). Non-autologous cells, such as xenogeneic or xenogeneic cells, can be engineered ex vivo to express HLA class I and class II molecules that match known human HLA specificities. These cells can then be introduced into a human subject that matches the HLA specificity of the engineered cells. The cells can be further engineered ex vivo to express one or more LAMP constructs according to the present invention.

[0210] The engineered cells are maintained in cell culture using standard cell culture techniques (Darling, Animal Cells: Culture and Media, J. Wiley, New York, 1994; Freshney, Culture of Animal Cells, Alan R. Liss, Inc., New York, 1987). Cell lines for use in the present invention can be obtained from a variety of sources (e.g., ATCC Catalogue of Cell Lines & Hybidomas, American Type Culture Collection, 8th edition, 1995) or generated using standard methods (Freshney, Culture of Immortalized Cells, Wiley-Liss, New York, 1996). Non-transformed cell lines are preferred for use in human subjects.

[0211] In one embodiment, CD34+ precursors that differentiate into dendritic cells under the influence of GM-CSF are obtained from the subject's body, and a nucleic acid encoding the LAMP construct according to the present invention is introduced into the cells, which are then injected into the subject. Utilizing the improved LAMP constructs described herein, the binding of peptides derived from a specific antigen to MHC class II molecules on the transduced antigen-presenting cells is enhanced, resulting in stronger systemic T cell-dependent immune responses and / or antibody production. The antigen-presenting cells transfected in this strategy are preferably autologous cells, although any MHC class II cells that effectively present antigens in the host may be used as described above.

[0212] Peptide Vaccine Peptide vaccines encoded by the improved LAMP constructs are also within the scope of the present invention. Preferably, the antigen is processed within the compartment / organelle (or the subsequent compartment / organelle to which it is delivered) to generate epitopes capable of modulating an immune response bound to MHC class II molecules.

[0213] The peptide vaccine encoded by the improved LAMP construct can also be bound to a membrane structure to facilitate its administration to the body of an organism. For example, the peptide vaccine encoded by the improved LAMP construct can be incorporated into a liposome, as described in U.S. Patent No. 4,448,765.

[0214] When a protein or polypeptide is used as an immunogen, it can be produced by expressing any one or more of the improved LAMP constructs described herein in recombinant cells, or prepared by chemical synthesis. For example, the Merrifield technique (Journal of American Chemical Society, vol. 85, pp. 2149-2154, 1968) can be used.

[0215] Methods for producing antibodies using LAMP constructs The improved LAMP construct as a polynucleotide, the encoded protein of the improved LAMP construct, and / or cells (such as antigen-presenting cells expressing the improved LAMP construct described herein) can be used to generate antibodies by methods well known to those of skill in the art, such as methods described in the art. See, for example, Sutcliffe et al., supra; Wilson et al., supra; Chow et al., Proc. Natl. Acad. Sci. USA 82:910-914 (1985); and Bittle et al., J. Gen. Virol. 66:2347-2354 (1985). When in vivo immunization is used, animals can be immunized with a protein encoded by the improved LAMP construct and / or a polynucleotide comprising the improved LAMP construct comprising the antigen described herein. Priming with the improved LAMP constructs as polynucleotides, the encoded proteins of the improved LAMP constructs, and / or cells (such as antigen-presenting cells expressing the improved LAMP constructs described herein) followed by boosting with antigen is a preferred embodiment of the present invention. In a further preferred embodiment, priming with the improved LAMP constructs described herein followed by boosting with antigen is specifically contemplated and can be used to generate a more robust immune response, particularly in terms of antibody repertoire diversity and titer.

[0216] The improved LAMP construct containing the antigen can be injected into a non-human vertebrate to produce antibodies. The preparation of the LAMP construct and the injection into a non-human vertebrate can be accomplished according to the principles of animal immunization well known to those skilled in the art.

[0217] The use of improved LAMP constructs to effectively present antigens involves, in one embodiment, antigens that are processed by LAMP in antigen-presenting cells after endocytosis and fusion of endosomes with lysosomes. The endosomes fuse with exocytic vesicles from the Golgi apparatus that contain class II MHC molecules, and the resulting peptides are bound. The MHC-peptide complexes are then delivered to the CD4+ It is transported to the plasma membrane where it is available for display to T cells.

[0218] Animals such as rabbits, rats, mice, llamas, camels, and / or cows can be immunized with the improved LAMP constructs containing the antigen and / or polynucleotides encoding the improved LAMP constructs containing the antigen. Additional animals suitable for immunization include non-human mammals, such as rodents (e.g., guinea pigs, hamsters, rats, mice), murines (e.g., mice), canines (e.g., dogs), felines (e.g., cats), equines (e.g., horses), primates, anthropoids (e.g., monkeys or apes), monkeys (e.g., marmosets, baboons, rhesus monkeys), apes (e.g., gorillas, chimpanzees, orangutans, gibbons).

[0219] For example, intraperitoneal and / or intradermal injection of an emulsion containing about 100 micrograms of antigen-containing modified LAMP construct or carrier protein and Freund's adjuvant or any other adjuvant known to stimulate immune response can be used. Several booster injections (such as those containing recombinant antigen protein) may be required, for example at intervals of about two weeks, to provide a useful titer of anti-antigen antibodies detectable by ELISA assay using free antigen directly or indirectly (e.g., via biotinylated AviTag) adsorbed to a solid surface. The titer of anti-antigen antibodies in the serum of immunized animals can be increased by the selection of anti-antibodies, for example, by adsorption to antigen on a solid support and elution of the selected antibodies by methods well known in the art.

[0220] Alternatively, the polynucleotide encoding the improved LAMP construct containing the antigen can be directly introduced into an animal.See, for example, U.S. Patent Nos. 5,676,954; 6,875,748; 5,661,133; Sahin et al., Nat Rev Drug Discov, 2014 Oct; 13(10): 759-80; Kariko et al., Mol Ther, 2008 Nov; 16(11): 1833-40; Kariko et al., Nucleic Acid Res, 2011, Nov; 39(21): e142; U.S. Patent No. 6,511,832.In one example, the improved LAMP construct containing the antigen is directly injected into a non-human vertebrate animal.Injection into an animal can be performed via intramuscular, intradermal, intranasal, subcutaneous, intravenous, intratracheal, and intrathecal delivery.Subsequent boosting with recombinant antigen can also be included in the generation of antibodies.

[0221] Additionally, antibodies generated by the disclosed methods can be affinity matured using display technologies such as, for example, phage display, yeast display, or ribosome display. In one example, single chain antibody molecules ("scFvs") displayed on the surface of phage particles are screened to identify scFvs that immunospecifically bind to an antigen and / or starting protein. The present invention encompasses both scFvs and portions thereof that have been identified as immunospecifically binding to an antigen and / or starting protein. Such scFvs can be routinely "converted" into immunoglobulin molecules, for example, by inserting nucleotide sequences encoding the VH and / or VL domains of the scFv into an expression vector containing constant domain sequences and engineering to direct the expression of the immunoglobulin molecule.

[0222] Recombinant expression of the produced antibodies (including scFvs and other molecules comprising or consisting of antibody fragments or variants thereof (e.g., heavy or light chains or portions thereof of the antibody of the invention, or single chain antibodies of the invention) using the improved LAMP constructs comprising antigens and / or polynucleotides encoding the improved LAMP constructs comprising antigens of the invention requires the construction of expression vector(s) comprising polynucleotides encoding the antibody or fragments or variants thereof. Once a polynucleotide encoding an antibody molecule (e.g., a whole antibody, a heavy or light chain of an antibody, or variants or portions thereof (preferably, but not necessarily, comprising a heavy or light chain variable domain)) is obtained, vector(s) for the production of the antibody molecule can be made by recombinant DNA technology using techniques well known in the art. Thus, methods for preparing antibodies by expressing a polynucleotide comprising an antibody-encoding nucleotide sequence are described herein. Methods well known to those skilled in the art can be used to construct expression vectors comprising antibody coding sequences and appropriate transcriptional and translational control signals. These methods include, for example, in vitro recombinant DNA techniques, synthetic techniques, and in vivo genetic recombination, as described herein. Thus, the present invention provides a replicable vector comprising a nucleotide sequence operably linked to a promoter encoding an isolated anti-antigen antibody (e.g., a whole antibody, a heavy or light chain of the antibody, a heavy or light chain variable domain of the antibody, or a portion thereof, or a heavy or light chain CDR, a single chain Fv, or a fragment or variant thereof) obtained as described herein. Such vectors include nucleotide sequences encoding the constant region of an antibody molecule (see, e.g., PCT Publication No. WO 86 / 05807; PCT Publication No. WO 89 / 01036; and U.S. Patent No. 5,122,464), and the variable domain of the antibody can be cloned into such a vector for expression of the whole heavy chain, the whole light chain, or both the whole heavy and light chains.

[0223] The expression vector(s) can be transferred into a host cell by conventional techniques, and the transfected cells are then cultured by conventional techniques to produce any anti-antigen antibody. Thus, the present invention includes a host cell comprising a polynucleotide operably linked to a heterologous promoter that encodes an anti-antigen antibody (e.g., a whole antibody, a heavy or light chain thereof, or a portion thereof, or a single chain antibody of the present invention, or a fragment or variant thereof). In a preferred embodiment, for expression of a whole antibody molecule, vectors encoding both the heavy and light chains can be co-expressed in the host cell for expression of a whole immunoglobulin molecule, as described in detail below.

[0224] A variety of host-expression vector systems can be utilized to express the anti-antigen antibody. Such host-expression systems represent vehicles in which the coding sequence of interest can be produced and subsequently purified, but also represent cells that express the anti-antigen antibody when transformed or transfected with the appropriate nucleotide coding sequence. These include, but are not limited to, microorganisms such as bacteria (e.g., E. coli, B. subtilis) transformed with a recombinant bacteriophage DNA, plasmid DNA, or cosmid DNA expression vector containing the sequence; yeast (e.g., Saccharomyces, Pichia) transformed with a recombinant yeast expression vector containing the coding sequence; insect cell systems infected with a recombinant viral expression vector (such as a baculovirus) containing the coding sequence; plant cell systems infected with a recombinant viral expression vector (e.g., Cauliflower Mosaic Virus, CaMV; Tobacco Mosaic Virus, TMV) or transformed with a recombinant plasmid expression vector (e.g., a Ti plasmid) containing the coding sequence; or mammalian cell systems (e.g., COS, CHO, BHK, 293, 3T3 cells) containing a recombinant expression construct containing a promoter derived from the genome of a mammalian cell (e.g., a metallothionein promoter) or a mammalian virus (e.g., an adenovirus late promoter; a vaccinia virus 7.5K promoter). Preferably, bacterial cells such as Escherichia coli, more preferably eukaryotic cells, are used to express the anti-antigen antibody. For example, mammalian cells such as Chinese hamster ovary cells (CHO) are effective expression systems in combination with vectors such as the major intermediate early gene promoter element from human cytomegalovirus (Foecking et al., Gene 45:101 (1986); Cockett et al., Bio / Technology 8:2 (1990)).

[0225] In bacterial systems, many expression vectors can be advantageously selected depending on the intended use. For example, when producing large amounts of protein (either for antibody production or for encoding polypeptides of the improved LAMP construct), a vector that directs the expression of high levels of a fusion protein product that is easily purified may be desirable. Such vectors include, but are not limited to, the E. coli expression vector pUR278 (Ruther et al., EMBO 1.2:1791 (1983)), in which coding sequences can be individually ligated into the vector in frame with the lac Z coding region such that a fusion protein is produced; pIN vectors (Inouye & Inouye, Nucleic Acids Res. 13:3101-3109 (1985); Van Heeke & Schuster, J. Biol. Chem. 24:5503-5509 (1989)), and the like. pGEX vectors can also be used to express foreign polypeptides as fusion proteins with glutathione 5-transferase (GST). In general, such fusion proteins are soluble and can easily be purified from lysed cells by adsorption and binding to matrix glutathione agarose beads followed by elution in the presence of free glutathione. The pGEX vectors are designed to contain thrombin or factor Xa protease cleavage sites so that the cloned target gene product can be released from the GST moiety.

[0226] In an insect system, Autographa californica nuclear polyhedrosis virus (AcNPV) can be used as a vector to express the encoded polypeptides of anti-antigen antibodies or improved LAMP constructs. The virus grows in the cells of Spodoptera frugiperda. Coding sequences are cloned individually into non-essential regions of the virus (e.g., the polyhedrin gene) and placed under control of an AcNPV promoter (e.g., the polyhedrin promoter).

[0227] In mammalian host cells, many virus-based expression systems can be used to express the coding polypeptide of anti-antigen antibody or improved LAMP construct. When adenovirus is used as an expression vector, the coding sequence of interest can be ligated to the adenovirus transcription / translation control complex, such as the late promoter and tripartite leader sequence. This chimeric gene can then be inserted into the adenovirus genome by in vitro or in vivo recombination.

[0228] Insertion into a non-essential region of the viral genome (e.g., region E1 or E3) results in a recombinant virus capable of expressing the encoded polypeptide of the anti-antigen antibody or improved LAMP construct that is viable in an infected host (see, e.g., Logan & Shenk, Proc. Natl. Acad. Sci. USA 8 1:355-359 (1984)).

[0229] Specific initiation signals may also be required for efficient translation of inserted coding sequences. These signals include the ATG initiation codon and adjacent sequences. Furthermore, the initiation codon must be in phase with the reading frame of the desired coding sequence to ensure translation of the entire insert. These exogenous translational control signals and initiation codons can be of a variety of origins, both natural and synthetic. The efficiency of expression can be enhanced by the inclusion of appropriate transcription enhancer elements, transcription terminators, etc. (see, e.g., Bittner et al., Methods in Enzymol. 153:51-544 (1987)).

[0230] In addition, a host cell line can be selected that modulates the expression of the inserted sequence or modifies and processes the gene product in a desired specific manner. Such modifications (e.g., glycosylation) and processing (e.g., cleavage) of protein products can be important for the function of the protein. Different host cells have characteristic and specific mechanisms for post-translational processing and modification of proteins and gene products. An appropriate cell line or host system can be selected to ensure correct modification and processing of the expressed foreign protein; for this purpose, eukaryotic host cells that possess the cellular machinery for proper processing of the primary transcript, glycosylation and phosphorylation of the gene product can be used. Such mammalian host cells include, but are not limited to, CHO, VERY, BHK, Hela, COS, NSO, MDCK, 293, 3T3, W138, and in particular breast cancer cell lines such as BT483, Hs578T, HTB2, BT2O and T47D, and normal mammary gland cell lines such as CRL7O3O and HsS78Bst.

[0231] For long-term, high-yield production of recombinant proteins, stable expression is preferred. For example, cell lines can be engineered to stably express the expression of anti-antigen antibodies or the encoded polypeptide of an improved LAMP construct. Rather than using expression vectors containing viral origins of replication, host cells can be transformed with a polynucleotide controlled by appropriate expression control elements (promoter, enhancer, sequences, transcription terminators, polyadenylation sites, etc.) and a selectable marker. Following introduction of the foreign polynucleotide, engineered cells can be grown in enriched medium for 1-2 days and then switched to selective medium. The selectable marker on the recombinant plasmid confers resistance to selection, allowing cells to grow with the plasmid stably integrated into their chromosomes and form foci that can be cloned and propagated within the cell line. This method can be advantageously used to engineer cell lines to express the encoded polypeptide of anti-antigen antibodies or the improved LAMP construct.

[0232] A number of selection systems can be used, including but not limited to, the genes for herpes simplex virus thymidine kinase (Wigler et al., Cell 11:223 (1977)), hypoxanthine guanine phosphoribosyltransferase (Szybalska & Szybalski, Proc. Natl. Acad. Sci. USA 48:202 (1992)), and adenine phosphoribosyltransferase (Lowy et al., Cell 22:8 17 (1980)), in tk-, hgprt-, or aprt- cells, respectively. Antimetabolite resistance can also be used as the basis for selection of the following genes: dhfr, which confers resistance to methotrexate (Wigler et al., Natl. Acad. Sci. USA 77:357 (1980); O'Hare et al., Proc. Natl. Acad. Sci. USA 78:1527 (1981)); gpt, which confers resistance to mycophenolic acid (Mulligan & Berg, Proc. Natl. Acad. Sci. USA 78:2072 (1981)); neo, which confers resistance to the aminoglycoside G-418 (Goldspiel et al., Clinical Pharmacy, 12:488-505 (1993); Wu and Wu, Biotherapy, 12:131-135 (1994)). 3:87-95 (1991); Tolstoshev, Ann. Rev. Pharmacol. Toxicol. 32:573-596 (1993); Mulligan, Science 260:926-932 (1993); and Morgan and Anderson, Ann. Rev. Biochem. 62:191-217 (1993); TIB TECH 11(5):155-215 (May; 1993)); and hygro, which confers resistance to hygromycin (Santerre et al., Gene 30:147 (1984)).Methods commonly known in the field of recombinant DNA technology can be routinely applied to select the desired recombinant clones, and such methods are described, for example, in Ausubel et al. (eds.), Current Protocols in Molecular Biology, John Wiley & Sons, NY (1993); Kriegler, Gene Transfer and Expression, A Laboratory Manual, Stockton Press, NY (1990); and Chapters 12 and 13, Dracopoli et al. (eds), Current Protocols in Human Genetics, John Wiley & Sons, NY (1994); Colberre-Garapin et al., J. Mol. Biol. 150:1 (1981).

[0233] The expression level of the anti-antigen antibody or the polypeptide encoded by the improved LAMP construct can be increased by vector amplification (for a review, see Bebbington and Hentschel, The Use Of Vectors Based On Gene Amplification For The Expression Of Cloned Genes In Mammalian Cells In DNA Cloning, Vol. 3. (Academic Press, New York, 1987)). If the vector-based marker expressing the polypeptide encoded by the anti-antigen antibody or the improved LAMP construct is amplifiable, increasing the level of inhibitor present in the host cell culture increases the copy number of the marker gene. Since the amplified region is associated with the coding sequence, the expression of the anti-antigen antibody or the production of the polypeptide encoded by the improved LAMP construct also increases (Crouse et al., Mol. Cell. Biol. 3:257 (1983)).

[0234] Other elements that can be included in the vector sequence include, but are not limited to, heterologous signal peptides (secretion signals), membrane anchoring sequences, introns, alternative splice sites, translation start and stop signals, inteins, biotinylation sites and other sites that facilitate post-translational modification, purification tags, sequences encoding fusions to other proteins or peptides, separate coding regions separated by internal ribosome re-entry sites, sequences encoding "marker" proteins that confer, for example, selectability (e.g., antibiotic resistance) or sortability (e.g., fluorescence), modified nucleotides and other known polynucleotides with cis-acting features.

[0235] A host cell can be co-transfected with two expression vectors of the invention, for example, a first vector encoding a heavy chain derived polypeptide and a second vector encoding a light chain derived polypeptide. The two vectors can contain identical selectable markers that allow for equal expression of heavy and light chain polypeptides. Alternatively, a single vector can be used that can encode and express both heavy and light chain polypeptides. In such a situation, it is preferred that the light chain is placed before the heavy chain to avoid excess non-toxic heavy chain (Proudfoot, Nature 322:52 (1986); Kohler, Proc. Natl. Acad. Sci. USA 77:2 197 (1980)). The coding sequences for the heavy and light chains can comprise cDNA or genomic DNA or synthetic DNA sequences.

[0236] Once the encoded polypeptide of the anti-antigen antibody or improved LAMP construct is produced by recombinant expression, it can be purified by any method known in the art for the purification of proteins, such as chromatography (e.g., ion exchange, affinity (particularly by affinity of Protein A and immunoaffinity for the specific antigen), and sizing column chromatography), centrifugation, differential solubility, or any other standard technique for protein purification. Additionally, the encoded polypeptide of the anti-antigen antibody or improved LAMP construct can be fused to heterologous polypeptide sequences described herein or known in the art to facilitate purification.

[0237] In one example, the encoded polypeptide of the anti-antigen antibody or improved LAMP construct may be fused to the constant domain of an immunoglobulin (IgA, IgE, IgG, IgM) or a portion thereof (CH1, CH2, CH3, or any combination and portion thereof), or albumin (including but not limited to recombinant human albumin or a fragment or variant thereof (e.g., U.S. Pat. No. 5,876,969 issued Mar. 2, 1999, European Patent No. 0413622, and U.S. Pat. No. 5,766,883 issued Jun. 16, 1998)), resulting in a chimeric polypeptide. Such fusion proteins may facilitate purification and extend half-life in vivo. This is shown as a chimeric protein consisting of the first two domains of the human CD4-polypeptide and various domains of the constant region of the heavy or light chain of a mammalian immunoglobulin. See, for example, European Patent No. 394,827; Traunecker et al., Nature, 331:84-86 (1988). Enhancement of antigen delivery across epithelial barriers to the immune system has been demonstrated for antigens (e.g., insulin) bound to FcRn binding partners such as IgG or Fe fragments (see, for example, PCT Publications WO 96 / 22024 and WO 99 / 04813). IgG fusion proteins with disulfide-linked dimeric structures through disulfide bonds in the IgG portion have been found to be more efficient in binding and neutralizing other molecules than monomeric polypeptides or fragments thereof alone. See, for example, Fountoulakis et al., J.Biochem., 270:3958-3964 (1995). Nucleic acids encoding anti-antigen antibodies or the encoding polypeptides of the improved LAMP constructs described herein can also be recombined with the gene of interest as an epitope tag (e.g., a hemagglutinin ("HA") tag or a Flag tag) to aid in detection and purification of the expressed polypeptide. For example, a system described by Janknecht et al. allows for the ready purification of non-denatured fusion proteins expressed in human cell lines (Janknecht et al., 1991, Proc. Natl. Acad. Sci. USA 88:8972-897).In this system, a gene of interest is subcloned into a vaccinia recombination plasmid such that the gene's open reading frame is translationally fused to an amino-terminal tag consisting of six histidine residues. The tag serves as the matrix-binding domain of the fusion protein. Extracts from cells infected with recombinant vaccinia virus are loaded onto Ni2+ nitriloacetic acid-agarose columns, and histidine-tagged proteins can be selectively eluted with imidazole-containing buffers.

[0238] Administration Vaccine material according to the invention may comprise an improved immunostimulatory LAMP construct as described herein, or may be a recombinant microorganism, or an antigen-presenting cell expressing an improved immunostimulatory LAMP construct. Preparation of improved LAMP constructs comprising vaccine material according to the invention and administration of such improved LAMP constructs for immunization of an individual is accomplished according to principles of immunization well known to those skilled in the art.

[0239] These materials can be obtained in large quantities by culturing recombinant or transformed cells containing replicons expressing the improved LAMP constructs described herein. Cultivation methods are well known to those skilled in the art and are taught in one or more of the documents cited above. The improved LAMP construct vaccines are generally produced by culturing recombinant or transformed cells and formulated into pharmacologically acceptable solutions or suspensions, which are usually physiologically compatible aqueous solutions, or coated tablets, tablets, capsules, suppositories or ampoules as described in the art, for example, in U.S. Pat. No. 4,446,128, which is incorporated herein by reference. Administration can be by any suitable route, oral, rectal, intranasal or by injection, which can be, for example, transdermal, subcutaneous, intramuscular or intravenous.

[0240] The improved LAMP construct is administered to the mammal in an amount sufficient to induce an immune response in the mammal. The preferred minimum amount for administration is the amount necessary to induce antibody formation to a concentration at least four times that present prior to administration. Typical initial doses for administration are 10 to 5000 micrograms for intravenous, intramuscular or subcutaneous administration, or 10 5 ~10 11 The amount of plaque-forming unit recombinant vector may be adjusted by the clinician administering it, as is the case with administering vaccines and other agents that induce an immune response. Usually, a single dose may be sufficient to induce immunity, but multiple doses may be administered to ensure or boost the response.

[0241] The improved LAMP construct vaccine can be first tested in non-human mammals (e.g., mice or primates). For example, an assay of immune response in vaccinated mice can be used to show more antibody, T cell proliferation, and cytotoxic T cell responses to the improved LAMP construct than to the wild-type antigen. The improved LAMP construct can be evaluated in rhesus monkeys to determine whether the vaccine formulation that is highly effective in mice elicits an adequate monkey immune response. In one embodiment, each monkey is administered a total of 5 mg of DNA per immunization, divided into two sites by IM delivery, and immunized on day 0 and weeks 4, 8, and 20, with additional doses being optional. Antibody responses, ADCC, CD4+ and CD8+ T cell cytokine production, and CD4+ and CD8+ T cell antigen-specific cytokine staining can be measured to monitor the immune response to the vaccine.

[0242] Further description of suitable methods of formulation and administration according to the present invention can be found in U.S. Pat. No. 4,454,116 (constructs), U.S. Pat. No. 4,681,762 (recombinant bacteria), and U.S. Pat. Nos. 4,592,002 and 4,920,209 (recombinant viruses).

[0243] Cancer immunotherapy: Candidates for prevention and treatment Candidates for cancer immunotherapy are any cancer patients who will be treated with any of the improved LAMP constructs described herein.Examples include patients with documented Epstein-Barr virus-associated lymphoma, HPV-associated cervical cancer, chronic HCV, or patients with distinct rearrangements or mutations in oncogenes or tumor suppressor genes.

[0244] In preferred embodiments, cancers treatable using the vaccines described herein include, but are not limited to, adenocarcinoma of all advanced stages, sarcoma, skin cancer, melanoma, bladder cancer, brain cancer, breast cancer, uterine cancer, ovarian cancer, prostate cancer, lung cancer (including but not limited to NSCLC, SCLC, squamous cell carcinoma), colorectal cancer, anal cancer, rectal cancer, cervical cancer, liver cancer, head and neck cancer, oral cancer, salivary gland cancer, esophageal cancer, pancreatic cancer, pancreatic ductal adenocarcinoma (PDA), renal cancer, gastric cancer, renal cancer, multiple myeloma or brain cancer.

[0245] It is envisioned that treatment with vaccine compositions containing the improved LAMP constructs could be used at any time during the course of an individual's cancer once it has been identified, and high-risk patients could also be vaccinated to prevent subsequent cancer development.

[0246] Treatment Procedure In one embodiment, the improved LAMP construct can be injected into the patient at any suitable time during the course of malignant tumor. For example, the improved LAMP construct is injected at a stage when the tumor burden is low. In an alternative embodiment in which the improved LAMP construct is introduced into the antigen-presenting cells of an individual, antigen-presenting cells or precursors of mature antigen-presenting cells are harvested from either the bone marrow or peripheral blood of an individual by venipuncture. These cells are established in culture and transduced with the improved LAMP construct. Once transduced, these antigen-presenting cells are infused back into the patient.

[0247] In a particularly preferred embodiment, the present invention provides a method for treating cancer patients with low tumor burden, such as early in the disease, after resection of a neoplastic tumor, or when tumor cell burden is reduced. In this method, a cell population is obtained from the patient, including autologous stem cells capable of differentiating into antigen-presenting cells expressing MHC class II molecules. These cells are cultured and transformed to deliver antigens associated with MHC class II molecules either into this compartment / organelle or into another compartment / organelle where the antigen is delivered, by introducing an improved LAMP construct.

[0248] The transfected stem cell population is then reintroduced into the patient, where the stem cells react with the T h They differentiate into antigen-presenting cells that express MHC class II molecules complexed with epitopes, and the immune response to antigens is enhanced by enhancing the stimulation of helper T cell populations.

[0249] More generally, in one embodiment, the present invention provides vaccine compositions comprising improved LAMP constructs for modulating a mammalian immune response to an antigen (i.e., stimulating, enhancing or reducing such a response).

[0250] kit The present invention further includes a kit that facilitates the practice of the methods described herein. In one embodiment, the kit includes an improved LAMP construct as described herein and a cell for receiving the improved LAMP construct. The kit may further include one or more nucleic acids for engineering the cell into a professional APC. However, in one embodiment, the cell is a professional APC. The cell may or may not express a costimulatory molecule. In a preferred embodiment, if the cell does not express a costimulatory molecule, the antigen encoded by the improved LAMP construct is an autoantigen. In another embodiment, a panel of cells expressing different MHC molecules (e.g., known to be expressed in humans) is provided. In a further embodiment, the kit includes reagents (e.g., lipid-based formulations, viral packaging materials, cells, etc.) that facilitate the entry of the improved LAMP construct into cells. In yet a further embodiment, one or more T cell lines specific for the antigen encoded by the improved LAMP construct are provided to validate the ability of the improved LAMP construct to induce, modulate, or enhance an immune response. EXAMPLES

[0251] The invention will now be further described with reference to the following examples, it being understood that the following are merely examples and that details can be varied whilst still falling within the scope of the invention.

[0252] Example 1 - Construction of LAMP constructs The improved LAMP constructs shown in Figure 1 can be constructed using standard molecular biology techniques well known to those skilled in the art. For example, plasmids containing polynucleotides can be designed to generate the different structures of ILC-1 to ILC-6 shown in Figure 1. The LAMP domains shown in Figure 1 are derived from the amino acid sequences shown in Figures 3-10. Preferably, the LAMP domains are derived from the human LAMP proteins shown in Figures 3-10. It is also envisioned to clone corresponding domains from orthologous sequences by comparing with the human sequence to identify equivalent domains. Antigens of interest (including one or more antigens of interest) can be cloned individually or in combination into the described LAMP constructs.

[0253] Example 2 - Evaluation of immune responses in mice to LAMP constructs The ability of the improved LAMP construct described in Example 1 can be tested for its ability to modulate immune responses. For example, female BALB / c mice can be immunized with 50 μg of the improved LAMP construct and 5 μg of GMCSF in 100 μl PBS using Nanopass on days 0, 14, and 28. The experiment was terminated 4 weeks after the last administration.

[0254] Splenocytes (3x105 / well) are stimulated with antigen protein (10ug / ml) in T cell medium (RPMI with 10% heat inactivated FBS, 1% penicillin / streptomycin, and 1x2-ME) and supernatants are collected after 72 hours. Supernatants are diluted (400ul of supernatant + 200ul of T cell medium) and cytokines are assessed by ELISA. IL-10 or IL-4 production can be measured by ELISPOT assay.

[0255] Example 3 - Improved antigen presentation using LAMP constructs Survivin is the smallest member of the inhibitor of apoptosis (IAP) family of proteins involved in the inhibition of apoptosis and the regulation of the cell cycle. These functional attributes make it a unique protein that displays diverse functions, namely the regulation of cell proliferation and cell death. Survivin expression in tumors correlates not only with the inhibition of apoptosis and reduced rates of cell death, but also with resistance to chemotherapy and tumor aggressiveness [1-6]. Thus, survivin is an important target for cancer vaccines and therapeutics [7-9]. Survivin has been found to be predominantly expressed in both human and embryonic stem cells, as well as many somatic stem cell types, indicating a still unexplored role in stem cell generation and maintenance.

[0256] Cancer is a heterogeneous group of diseases in which abnormal cell proliferation that can invade other body parts controls normal homeostasis and become fatal if not treated timely and appropriately. Immunotherapy specifically targets tumor cells, thereby avoiding collateral damage to non-tumor cells, and induces an anti-tumor response. This anti-tumor response also has the potential to eradicate tumors at distant sites in the body, where surgical resection may not be possible. Inducing or enhancing an anti-tumor immune response is a formidable challenge in cancer, as tumor cells use multiple evasion strategies to avoid detection or elimination by immune cells.

[0257] The aim of this project is to evaluate the in vivo immune response of all new generation LAMP constructs injected ID into BALB / c mice. Specifically, mice were immunized with 50 μg of the test constructs defined in the legend of Fig. 1 by intradermal injection. No adjuvant was added in this experiment. Six mice / group were administered the vaccine every 7 days for a total of three doses per month. The immune response was monitored 14 days after the last immunization.

[0258] Test LAMP constructs were generated as described herein, and the sequence of each test construct is shown in FIG. 19. Survivin protein was purchased from MyBiosource (San Diego, CA). Survivin peptide was from GenScript (Piscataway, NJ). Anti-survivin and m-IgGk-BP-HRP were purchased from Santa Cruz Biotechnology (Dallas, TX), and mouse monoclonal anti-LAMP-1 / CD107a was purchased from OriGene Technologies (Rockville, MD). ELISPOT antibody pair against IFNγ was from Biolegend. Fluorescently conjugated CD3, CD4, CD8, CD44, CD62L, IFNγ, TNFα, Granzyme B, CD69 monoclonal antibodies and Zombie aqua fixable viability kit were purchased from BioLegend (San Diego, CA). Goat anti-mouse IgG2a-HRP and goat anti-mouse IgG-HRP were purchased from Southern Biotechnologies (Birmingham, AL). Streptavidin-HRP was purchased from Thermo Fisher (Waltham, MA). SureBlue TMB Microwell Peroxidase Substrate and TMB Stop Solution were purchased from KPL (Gaithersburg, MD).

[0259] 50 μg of each construct was used in Pharmajet in a total volume of 100 ul / mouse / dose. Mice were immunized with the vaccine by id delivery on days 0, 7, and 14. Mice were bled on day 28 for serum collection. Serum was collected and stored at -30°C. Spleens were collected at the end of the experiment on day 28 and processed by ELISPOT and FACS to evaluate survivin-specific T cell responses.

[0260] Measurement of plasma survivin-specific total IgG by ELISA. Murine antibody responses to survivin were assessed by indirect ELISA. ELISA plates (MaxiSorp) were coated overnight with 2 μg / ml survivin (1-142) protein in carbonate-bicarbonate buffer and blocked with 2% BSA in PBS. Plasma samples were diluted 1:100 in blocking buffer. Samples were detected with goat anti-mouse IgG-HRP (Southern Biotech, Birmingham, AL). Reactions were developed with SureBlue TMB substrate and stopped with TMB stop solution from KPL (Gaithersburg, MD). Plates were read (OD450) using an Epoch ELISA reader (BioTek, Winooski, VT).

[0261] Assessment of antigen-specific T cell responses. To assess antigen-specific T cell responses in vaccinated mice, splenocytes from vaccinated mice were assessed for antigen-specific IFNγ production by enzyme-linked immunospot (ELISPOT). For the ELISPOT assay, 96-well nitrocellulose plates (Millipore) were coated overnight at 4°C with 100 μl / well capture monoclonal antibody in PBS. Plates were washed three times with 200 μl / well PBS and blocked with 200 μl / well T cell medium at room temperature for at least 2 h. Splenocytes were cultured at 3×10 5 Cells were seeded at 1000 cells / well and incubated with 2 μg / ml of pooled survivin peptides (Table 1), or concavalin A (0.125 μg / ml), or with medium alone in a total volume of 200 μl / well of T cell medium (L-glutamine and HEPES (ATCC), 1% penicillin, 1% streptomycin, and 5 × 10 -5 3 × 10 5 Cells / well at 37°C, 5% CO 2The plates were co-cultured for 48 h at 4 °C for 1 h. The plates were washed twice with 200 μl / well PBS and twice with 200 μl / well PBS-T (0.05% Tween / PBS). Diluted detection antibody (50 μl / well in PBS-T / 0.5% BSA) was added and the plates were incubated for 2 h at room temperature with shaking. The plates were washed four times with PBS. Streptavidin-alkaline phosphatase (50 μl / well) diluted in PBS was added and incubated for 2 h. The plates were washed four times with PBS and developed with 50 μl / well 3-amino-9-ethylcarbazole (AEC, BD Bioscience) substrate for 10 min. Development was stopped by washing with running tap water. After drying for 72 h at room temperature in the dark, the colored spots were counted using an AID ELISPOT high resolution reader system and AID ELISPOT software version 3.5 (Autoimmun Diagnostika GmbH).

[0262] Table 1. Pooled peptides from Genscript

[0263] [Table 1]

[0264] Western Blot. 293T cells were transfected with the test constructs using Lipofectamine 2000 reagent (Invitrogen). Transfected cells were washed with PBS and suspended in 200 μl of RIPA lysis buffer containing stop proteinase inhibitor (Thermo Scientific, Waltham, MA). Lysates were centrifuged (700 g, 15 min at 4° C.) and the protein concentration of the clarified supernatant was then measured using the Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Waltham, MA). 10 μg of protein was electrophoresed on a precast (4-20%) SDS-PAGE gel (BioRad, Hercules, California), transferred to a nitrocellulose membrane (BioRad), and immunoblotted with mAb against hLAMP. Membranes were blocked with Detection™ Blocking Buffer (KPL), probed with rabbit anti-human LAMP (Sino Biological Inc., Beijing, China) or anti-survivin antibody and goat anti-rabbit HRP antibody, and developed with TMB (KPL).

[0265] Flow cytometry. Cells were first labeled with Zombie aqua fixable viability dye in PBS (1:500 dilution), followed by surface antibodies (1:100 dilution) in staining buffer (4% FBS, 2% rat serum, 2% mouse serum in PBS). For intracellular staining, cells were stained with Zombie aqua, followed by surface staining, fixed with 4% paraformaldehyde, and stained with intracellular antibodies in permeabilization buffer (PBS with 1% FCS, 0.1% saponin). Samples were analyzed on a CytoFlex flow cytometer (Beckman Coulter) and analyzed using Kaluza software (Beckman Coulter).

[0266] Statistics. To assess statistical significance, two-way ANOVA tests were performed using GraphPad Prism 6.0 software or R files. The RPMI results for each mouse were subtracted from the results of antigen activation.

[0267] Study design.

[0268] [Table 2]

[0269] Figure 14: Plasmid validation: 293T cells were transfected with plasmids for 3 days. Transfected cells were lysed and electrophoresed in precast SDS-PAGE gels. Proteins were transferred to nitrocellulose membranes and immunoblotted with mAbs against human LAMP (OriGene, #TA337108) or survivin (Santa Cruz #17779). Molecular weight of LAMP = 100 KD, survivin = 16 KD. Figure 13 shows that all tested LAMP constructs produced proteins of the appropriate size.

[0270] Figures 15 and 16: Tested LAMP constructs induce Th1 effector T cells producing IFNγ. Female BALB / c mice were immunized id with 50 μg of the indicated constructs in 100 μl PBS via the Pharmajet device on days 0, 7, and 14. The experiment was terminated 14 days after the last dose. Splenocytes (3x10 5 / well) were stimulated with survivin pool peptides (4 μg / ml) in T cell medium (RPMI with 10% heat-inactivated FBS, 1% penicillin / streptomycin, 1×β-ME) for 48 h. A. IFNγ production by spots. B. IFNγ production induced by all pool peptides (bar graph from A). n=6 / group. For statistical analysis, a two-way ANOVA (R file) was used. Figure 14 shows that all tested LAMP constructs induced robust T cell responses as indicated by IFNγ production.

[0271] After three doses of the improved LAMP construct (one week apart), it was unexpectedly found that a robust Th1-type response was induced by the tested LAMP constructs, especially by ILC-4, whose hinge sequence was replaced by the survivin gene. More interestingly, the improved LAMP construct ILC-4 appears to recognize the survivin epitope from the N-terminus to the C-terminus and induces a T cell response against the human survivin peptide sequence, which is 100% identical to the mouse. Also, a longer (72 hours) stimulation of frozen-thawed splenocytes with survivin peptide was found, and ILC-4 showed significantly higher IFNγ production than the first generation LAMP-survivin (see FIG. 19). Specifically, FIG. 16 shows that all tested improved LAMP constructs showed higher T cell responses, with ILC-4 having the highest activity, as this construct induced a significantly higher T cell response against all survivin peptide pools. Moreover, contrary to what was known in the art, removal of the second homologous domain of the luminal domain created an improved LAMP construct that elicited a more robust immune response compared to the complete LAMP construct (see results for ILC-2 and ILC-3). Frozen splenocytes (4x105 / well) were stimulated with pooled peptide 4 (4μg / ml) in T cell medium (RPMI with 10% heat-inactivated FBS, 1% penicillin / streptomycin, and 1xβ-ME) for 48 hours. n=6 / group. Two-way ANOVA was used for statistical analysis. *p<0.05, **p<0.01, ***p<0.005, ****p<0.0001

[0272] Figure 17. CD4 T cells are the major source of IFNγ producing cells. Female BALB / c mice were immunized id with 50 μg of the indicated vaccine in 100 μl of PBS via the Pharmajet device on days 0, 7, and 14. The experiment was terminated 14 days after the last dose. Splenocytes (1x10 6100 / well) were stimulated overnight with pool peptide 1 (4 μg / ml) in T cell medium (RPMI with 10% heat-inactivated FBS, 1% penicillin / streptomycin, and 1x β-ME) and cultured for an additional 5 h with the addition of monesin and brefeldin A. Cells were harvested and stained by Zombie, surface markers, and intracellular staining according to the ITI staining protocol. Cells were gated on memory CD4 T cells (CD4+CD44+CD62L-) or CD8 T cells (CD8+CD44+CD62L-). Data are one mouse representative of each group. Mice vaccinated with the various constructs have an increase in CD8 effector memory cells, but IFNγ production is more pronounced in the CD4 T cell population.

[0273] Figure 18: Improved LAMP constructs generated stronger survivin-specific total IgG responses in BALB / c mice. Female BALB / c mice were immunized id with 50 μg of the indicated vaccine in 100 μl PBS via a Pharmajet device on days 0, 7, and 14. The experiment was terminated 14 days after the last dose. Mice were bled on day 28. Serum was separated and stored at -30°C. Total IgG and IgG2a in serum were measured by ELISA. Briefly, ELISA plates were coated with 2 μg / ml survivin (1-142aa), blocked with PBS / 2% BSA, and serum (diluted 1:100 in blocking buffer) was assessed with HRP-labeled goat anti-mouse IgG (1:6000) and IgG2a (1:11000). n=6 mice / group. **p<0.01, ***p<0.005, ****p<0.0001. Importantly, and contrary to what was known in the art, FIG. 18 shows that fragments of the luminal domain worked better than using the complete luminal domain (i.e., comparing the complete LAMP construct with constructs ILC-2 and IL-3). Moreover, unexpectedly, inserting the antigen between the two homologous domains of the luminal domain generated the strongest antibody response (see ILC-4).

[0274] References cited in this section. 1.Kami K,Doi R,Koizumi M,Toyoda E,Mori T,Ito D,et al.Survivin expression is a prognostic marker in pancreatic cancer patients.Surgery.2004;136(2):443-8.doi:10.1016 / j.surg.2004.05.023.PubMed PMID:15300213. 2.Zhang SQ,Qiang SY,Yang WB,Jiang JT,Ji ZZ.[Expression of survivin in different stages of carcinogenesis and progression of breast cancer].Ai Zheng.2004;23(6):697-700.PubMed PMID:15191674. 3.Zhang X,Zhong L,Hu K,Li Q.[Expression of survivin and its correlation with apoptosis in non-small cell lung cancer].Zhongguo Fei Ai Za Zhi.2004;7(2):138-41.doi:10.3779 / j.issn.1009-3419.2004.02.14.PubMed PMID:21215009. 4.Kishi H,Igawa M,Kikuno N,Yoshino T,Urakami S,Shiina H.Expression of the survivin gene in prostate cancer:correlation with clinicopathological characteristics,proliferative activity and apoptosis.J Urol.2004;171(5):1855-60.doi:10.1097 / 01.ju.0000120317.88372.03.PubMed PMID:15076293. 5.Asanuma K,Tsuji N,Endoh T,Yagihashi A,Watanabe N.Survivin enhances Fas ligand expression via up-regulation of specificity protein 1-mediated gene transcription in colon cancer cells.J Immunol.2004;172(6):3922-9.PubMed PMID:15004200. 6.Miyachi K,Sasaki K,Onodera S,Taguchi T,Nagamachi M,Kaneko H,et al.Correlation between survivin mRNA expression and lymph node metastasis in gastric cancer.Gastric Cancer.2003;6(4):217-24.doi:10.1007 / s10120-003-0255-2.PubMed PMID:14716515. 7.Badana AK,Chintala M,Gavara MM,Naik S,Kumari S,Kappala VR,et al.Lipid rafts disruption induces apoptosis by attenuating expression of LRP6 and survivin in triple negative breast cancer.Biomed Pharmacother.2017;97:359-68.doi:10.1016 / j.biopha.2017.10.045.PubMed PMID:29091885. 8. Cai JP, Wang YD, Zhang X, Xue HZ. [Expression of P16 and survivin in liver cancer and their clinical significance]. Zhonghua Gan Zang Bing Za Zhi. 2017;25(10):778-80. doi:10.3760 / cma.j.issn.1007-3418.2017.10.013. PubMed PMID: 29108210. 9. Cho HJ, Kim HR, Park YS, Kim YH, Kim DK, Park SI. Prognostic value of survivin expression in stage III non-small cell lung cancer patients treated with platinum-based therapy. Surg Oncol. 2015;24(4):329-34. doi:10.1016 / j.suronc.2015.09.001. PubMed PMID: 26690822. 10. Godinho RM, Matassoli FL, Lucas CG, Rigato PO, Goncalves JL, Sato MN, et al. Regulation of HIV-Gag expression and targeting to the endolysosomal / secretory pathway by the luminal domain of lysosomal-associated membrane protein (LAMP-1) enhance Gag-specific immune response. PLoS One. 2014;9(6):e99887. doi:10.1371 / journal.pone.0099887. PubMed PMID: 24932692; PubMed Central PMCID: PMCPMC4059647.

[0275] Example 4: Therapeutic treatment with the LAMP construct Female BALB / c mice can be inoculated subcutaneously with 7000 syngeneic 4T1 breast cancer cells on day 0. Once tumors are palpable, 50ug of vaccine and 5ug of GMCSF in 100ul PBS are administered id using a nanopass. Primary tumors are measured with calipers and tumor volumes are calculated using the formula p / 6(length x width)3 / 2. Mean tumor volume can be measured as a function of days after tumor inoculation. Kaplan-Meier plots can be used to show overall survival at endpoint.

[0276] Example 5 - Prime / Boost Protocol Herpesvirus entry mediator (HVEM), also known as tumor necrosis factor receptor superfamily member 14 (TNFRSF14) or CD270, is a human cell surface receptor of the TNF receptor superfamily. Recently, HVEM has been found to be highly expressed in hematopoietic cells and parenchymal cells, such as those in breast, melanoma, colorectal, and ovarian cancer, as well as in the intestinal epithelium. HVEM is a bidirectional protein that either inhibits or stimulates T cells through binding to BTLA or LIGHT (TNFSF14).

[0277] A DNA vaccine encoding HVEM-LAMP was generated to generate antibodies capable of blocking the inhibitory function of HVEM for tumor therapeutic applications. We hypothesized that LAMP promotes antibody responses by increasing the affinity of HVEM-specific antibodies and / or expanding the repertoire of B-cell epitopes of the HVEM protein. In this study, we compared the immunogenicity of HVEM-encoding plasmids containing LAMPs with those without LAMPs. HVEM sequence: HVEM amino acids 39-202 (SEQ ID NO:114) LPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAHLNGLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCAACRAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKCSWLVTKAGAGTSSSHWV

[0278] Plasmids encoding HVEM-LAMP and HVEM and recombinant HVEM proteins were designed by ITI and produced by NTC (Lincoln, NE). Polynucleotides encoding the following HVEM sequences were cloned into the improved LAMP constructs described herein:

[0279] Goat anti-mouse IgG-HRP was purchased from Southern Biotechnologies (Birmingham, AL). SureBlue TMB microwell peroxidase substrate and TMB stop solution were purchased from KPL (Gaithersburg, MD). ELISPOT plates were ordered from EMD Millipore (Billerica, MA, catalog number MAIPS4510). The IFN-γ antibody pair used in the ELISPOT was purchased from BioLegend (San Diego, CA), with clones AN18 and R46A2 used for coating and detection, respectively. Streptavidin-HRP and AEC substrate were purchased from BD Biosciences (San Jose, CA).

[0280] Six- to eight-week-old female Balb / c mice were purchased from Harlan Laboratories (Frederick, MA) and housed in the animal facility at Immunomic Therapeutics, Inc. (Rockville, MA). Mice (n=6) were treated with 10 μg / dose of HVEM-LAMP, HVEM, or LAMP vector control on days 0, 7, and 14 by Ichor electroporation IM delivery. On day 35, mice were boosted by ip injection with 5 μg of HVEM protein in the presence of alum. On days 28 and 49, mice were bled and serum was isolated for antibody detection. Mice were sacrificed on day 56, and splenocytes were tested for IFN-γ production by ELISPOT.

[0281] Then, the ELISA procedure according to Su et al., J of Immunol Res;(10):1-15(2016) was performed. Plates were coated with 5 μg / ml HVEM protein. Data were analyzed using Microsoft Excel and Prism 6 software.

[0282] The main objective of this study was to compare the antibody profiles of HVEM-LAMP and HVEM. On day 28, mice vaccinated with the HVEM-LAMP vaccine produced significantly higher levels of HVEM-specific IgG antibodies than the HVEM group (Figure 11). After protein boosting, HVEM-specific antibodies increased approximately 1000-fold in HVEM-immunized mice, with the average titer changing from 100 to 108,000. This result indicates that immune memory was induced by the HVEM DNA plasmid. HVEM DNA alone elicited only a minimal antibody response, whereas immune memory was rapidly recalled by protein boosting. On the other hand, the HVEM-LAMP group again showed significantly higher titers than the HVEM and LAMP groups. The average titer was 5-fold higher than the HVEM group, indicating the power of LAMP to enhance antibody responses (Figure 12).

[0283] Additionally, serum samples (day 49) from mice immunized with HVEM+LAMP or HVEM alone / boosted with HVEM protein were pooled and tested for peptide mapping. Twelve peptides were found to bind to the pooled serum (mouse IgG response), with 7 of the 12 peptides showing strong binding affinity. HVEM+LAMP alters the binding affinity of peptides 17, 24, 25, and 28 compared to HVEM alone, as shown in Figure 13. These changes may have physiological effects in protecting tumor growth.

[0284] In conclusion, the data from this study suggest that the two constructs were expressed in vivo and that LAMP significantly improved the humoral immune response.

[0285] Example 6: Antibody production from polypeptides Anti-antigen antibodies can be prepared in a variety of standard ways using injection into animals to produce antibodies. (See current protocol, Chapter 2.) For example, cells expressing an improved LAMP construct containing the antigen described herein are administered to a non-human vertebrate to induce the production of serum containing polyclonal antibodies. In a preferred method, a preparation of LAMP / antigen protein is prepared and purified to be substantially free of natural contaminants. Such a preparation is then introduced into a non-human vertebrate to produce polyclonal antisera of higher specific activity.

[0286] In the most preferred method, the anti-antigen antibody of the present invention is a monoclonal antibody (or a protein-binding fragment thereof). Such a monoclonal antibody can be prepared using hybridoma technology. (Kohler et al., Nature 256:495 (1975); Kohler et al., Eur. J. Immunol. 6:511 (1976); Kohler et al., Eur. J. Immunol. 6:292 (1976); Hammerling et al., in: Monoclonal Antibodies and T-Cell Hybridomas, Elsevier, NY, pp.563-681 (1981).) In general, such a procedure involves immunizing a non-human vertebrate (preferably rabbit, mouse, cow, camel, llama) with an improved LAMP construct containing an antigen, an encoded polypeptide of the improved LAMP construct containing an antigen, or more preferably, with an improved LAMP construct-expressing cell. Such cells can be cultured in any suitable tissue culture medium, however, it is preferred to culture the cells in Earle's modified Eagle's medium supplemented with 10% fetal bovine serum (inactivated at about 56° C.), about 10 g / l of non-essential amino acids, about 1,000 U / ml of penicillin and about 100 ug / ml of streptomycin.

[0287] The spleen cells of such a non-human vertebrate host (e.g., mouse) are removed and fused with a suitable myeloma cell line. Any suitable myeloma cell line can be used in accordance with the present invention, but it is preferred to use the parent myeloma cell line (SP20) available from ATCC™. After fusion, the resulting hybridoma cells are selectively maintained in HAT medium and cloned by limiting dilution as described by Wands et al. (Gastroenterology 80:225-232 (1981)). The hybridoma cells obtained by such selection are then assayed to identify clones that secrete antibodies capable of binding to the antigen.

[0288] It will be understood that Fab and F(ab')2 and other fragments of anti-antigen antibodies can be used according to the methods disclosed herein. Such fragments are typically produced by proteolytic cleavage using enzymes such as papain (to produce Fab fragments) or pepsin (to produce F(ab')2 fragments). Alternatively, secreted protein-binding fragments can be produced through the application of recombinant DNA technology. Or through synthetic chemistry.

[0289] For in vivo use of antibodies in humans, it may be preferable to use "humanized" chimeric monoclonal antibodies. Such antibodies can be produced using genetic constructs derived from hybridoma cells that produce the monoclonal antibodies described above. Methods for producing chimeric antibodies are known in the art. (For reviews, see Morrison, Science 229:1202 (1985); Oi et al., BioTechniques 4:214 (1986); Cabilly et al., U.S. Pat. No. 4,816,567; Taniguchi et al., EP 171496; Morrison et al., EP 173494; Neuberger et al., WO 8601533; Robinson et al., WO 8702671; Boulianne et al., Nature 312:643 (1984); Neuberger et al., Nature 314:268 (1985)).

[0290] Example 7: Use of Polynucleotides to Generate Polyclonal and Monoclonal Antibodies Methods for directly injecting polynucleotides into animals are well described in the art. See, e.g., U.S. Patent Nos. 5,676,954; 6,875,748; 5,661,133. For example, polynucleotides encoding improved LAMP constructs containing antigens can be injected into the quadriceps of restrained awake mice (female 6-12 week old BALB / c or nude, nu / nu (Harlan Sprague Dawley, Indianapolis, Ind.)). In one embodiment, 50 μg of polynucleotide in a 50 μl solution can be injected into mice using a disposable sterile plastic insulin syringe and a 28G1 / 2 needle with a plastic collar cut from a micropipette tip (Becton-Dickinson, Franklin Lakes, NJ, Cat. No. 329430) as described in Hartikka, J., et al., Hum. Gene Ther. 7:1205-1217 (1996).

[0291] Alternatively, 6-week-old Sprague Dawley female mice (20-25 grams body weight) may be given 5000 ppm ZnOSO4 in their drinking water for 24 hours prior to injection. This amount of zinc has been shown to be capable of activating the metallothionein promoter. Each mouse is then injected intravenously via tail vein puncture with a 25-gauge needle with 30 μg of polynucleotide encoding the modified LAMP construct containing the antigen and 150 μg of liposomes complexed with it (Lipofection TM) in a total volume of 30 μl. Animal care should be maintained throughout the study and should be performed in accordance with the “Guide for the Use and Care of Laboratory Animals”, Institute of Laboratory Animal Resources, Commission on Life Sciences, National Research Council, National Academy Press.

[0292] After the polynucleotide encoding the improved LAMP construct containing the injected antigen is delivered to the cells of the animal, the antigen is delivered to the endosome / lysosome, processed and presented to the immune system. The improved LAMP construct containing the antigen can then stimulate the production of antibodies specific to the antigen. These antibodies can be isolated and used as a polyclonal mixture, or can be further isolated into a single species or monoclonal. The process of immune response and the production of antibodies against foreign antigens in vivo are well known in the art.

[0293] In the third animal model, Balb / c 3T3 A31 cells are transfected by electroporation with a polynucleotide encoding the modified LAMP construct containing the antigen. G418-resistant clones expressing the LAMP construct containing the antigen are identified by their ability to bind to human RBCs. To generate polyclonal antibodies, Balb / c mice are intraperitoneally injected twice with 10 ng / mL of the modified LAMP construct containing the antigen at an interval of 14 days. 7 After the final boost, immune serum is collected, IgG is purified with protein G sepharose, and passed through an antigen column prepared by binding 1.0 mg of purified antigen to cyanogen bromide-activated sepharose CL-4B. Bound IgG can be eluted with 0.1 M glycine buffer pH 2.5 and neutralized with 0.1 volume of 0.1 M Tris pH 8.0. To generate monoclonal antibodies (mAbs), Balb / c mice are immunized with LAMP constructs containing the antigen, and hybridomas are generated by fusing immune splenocytes with SP2 myeloma according to standard methods (28). Positive wells that specifically react with the antigen can be identified by enzyme-linked immunosorbent assay as described in the art. Hybridomas are cloned three times by limiting dilution to produce antibodies.

[0294] Example 8: Immunization with modified LAMP constructs containing antigens Methods for producing antibodies in mammals are well known in the art. In one example, polyclonal antisera against the LAMP construct containing an antigen are produced by immunizing pathogen-free rabbits with a total of 500 μg of the improved LAMP construct containing the antigen for two months. For example, the improved LAMP construct containing the antigen can be dissolved in PBS and emulsified with an equal amount of Freund's adjuvant. After the final booster, the serum of the rabbit can be separated to determine the titer of the polyclonal antisera.

[0295] In an additional animal model, groups of five mice (C57BL / 6J; Jackson Labs) can be immunized subcutaneously with 5 μg of LAMP constructs containing endotoxin-free antigen emulsified in alum. Three weeks later, the mice can be bled and the serum titrated by ELISA to confirm the presence of anti-antigen-specific antibodies (antibodies in the serum are bound directly to wild-type BPTI or APP-KI that have been coated on wells, or indirectly (via a biotinylated tag and streptavidin)).

[0296] To obtain monoclonal antibodies, 4-6 week old Balb / c mice are immunized with the improved LAMP construct containing the antigen (e.g., 10-100 μg per injection, dissolved in Freund's complete adjuvant for the first injection and Freund's incomplete adjuvant for subsequent immunizations, 4 times at 2 week intervals). Splenocytes are isolated and fused with a fusion cell line, such as Sp2 / 0 myeloma cells, followed by limiting dilution. Growing clones are screened, for example, using enzyme-linked immunosorbent assay (ELISA). 96 cell plates are coated with the improved LAMP construct containing the antigen or a control protein. Culture supernatant is added, followed by washing and adding a labeled anti-mouse antibody for detection. After limiting dilution, cloning of anti-antigen antibodies producing stable hybridomas is obtained. Supernatants are collected from each cell, and monoclonal antibodies can be purified by affinity chromatography using a protein A sepharose column.

[0297] Variations, modifications, and other implementations of what is described herein will occur to those skilled in the art without departing from the spirit and scope of the invention and the claims. All patents, patent applications, international applications, and references identified are expressly incorporated herein by reference in their entirety.

Claims

1. A lysosomal associated membrane protein (LAMP) construct comprising two homologous domains of the luminal domain of the LAMP protein and an antigenic domain heterologous to said LAMP protein, said antigenic domain being located between said two homologous domains, The LAMP construct further comprises a transmembrane domain of a LAMP protein; A LAMP construct, wherein said LAMP protein is selected from human LAMP-1 or LAMP-2.

2. The LAMP construct of claim 1 further comprising a signal sequence.

3. The LAMP construct of claim 2 , wherein the signal sequence is derived from a LAMP protein.

4. A LAMP construct according to any one of claims 1 to 3, further comprising the cytoplasmic tail of the human LAMP-1 or LAMP-2 protein.

5. A LAMP construct according to any one of claims 1 to 4, wherein the antigenic domain is separated from one or both of the homologous domains by a linker.

6. The LAMP construct of claim 5, wherein the linker is selected from the amino acid sequences GPGPG or PMGLP.

7. The LAMP construct according to any one of claims 1 to 6, wherein said LAMP protein comprises an amino acid sequence selected from any one of SEQ ID NOs: 1 or 2.

8. The LAMP construct according to any one of claims 1 to 6, wherein the LAMP protein comprises an amino acid sequence that is at least 90%, 95%, 96%, 97%, 98% or 99% identical to at least one of SEQ ID NOs: 1 or 2.

9. The LAMP construct according to any one of claims 1 to 6, wherein said LAMP protein is human LAMP-1.

10. The LAMP construct according to any one of claims 1 to 6, wherein the two homology domains comprise human LAMP-1 homology domain 1 and human LAMP-1 homology domain 2.

11. The LAMP construct according to claim 10, wherein said human LAMP-1 homology domain 1 comprises the amino acid sequence of residues 29 to 194 of SEQ ID NO:

1.

12. The LAMP construct according to claim 10 or 11, wherein the human LAMP-1 homology domain 2 comprises the amino acid sequence of residues 228 to 381 of SEQ ID NO:

1.

13. The LAMP construct according to claims 9 to 12, wherein the transmembrane domain comprises residues 383 to 405 of SEQ ID NO:

1.

14. The LAMP construct according to any one of claims 9 to 13, wherein the cytoplasmic tail comprises residues 406 to 417 of SEQ ID NO:

1.

15. The LAMP construct of any one of claims 1 to 14, wherein the antigenic domain comprises survivin or HVEM.

16. The LAMP construct of claim 1, comprising the amino acid sequence of SEQ ID NO:

119.

17. A polynucleotide encoding the LAMP construct according to any one of claims 1 to 16.

18. A host cell comprising the polynucleotide of claim 17.

19. A composition comprising a LAMP construct according to any one of claims 1 to 16, a polynucleotide according to claim 17, or a host cell according to claim 18.

20. 20. The composition of claim 19 for alleviating or treating a disease or disorder, wherein the composition is administered to a subject in need thereof in an amount sufficient to alleviate or treat said disease or disorder.

21. The composition according to claim 20, which is used in a priming step and / or at least one boosting step.

22. The composition of claim 21 used in the priming step.

23. The composition of claim 21 or 22, wherein the at least one boosting step comprises administration of an antigen, a LAMP construct, a polypeptide encoded by a polynucleotide sequence of a LAMP construct, or a cell comprising a LAMP construct.

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