Method for decomposing polycyclic aromatic hydrocarbons using a complex decomposition bacterial consortium

Abstract

Provided is a method for decomposing polycyclic aromatic hydrocarbons using a complex decomposition bacterial consortium. [Solution] A method for decomposing polycyclic aromatic hydrocarbons using a decomposition bacterial flora complex is disclosed, which includes the following steps: first, decomposition bacterial flora is added to organic-contaminated water and allowed to react for 1 to 48 hours; then, a pre-complex is added and allowed to react continuously until the total time reaches 72 hours, so that the decomposition bacterial flora and the pre-complex form a complex system; this process can avoid the adverse effects of the pre-complex on the decomposition bacterial flora and successfully achieve decomposition; the present invention adds a decomposition bacterial flora and a pre-complex of calcium peroxide and ferrous sulfate heptahydrate as a complex system to polycyclic aromatic hydrocarbon-contaminated water, and can effectively remove polycyclic aromatic hydrocarbons through the decomposition reaction; compared with conventional microbial purification and oxidative purification, this method is efficient, environmentally friendly and has high applicability.

Description

[Technical field]

[0001] The present invention relates to the technical field of purification of polluted water areas, specifically, to a method for the purification of polycyclic aromatic hydrocarbons using a complex of decomposing bacteria. The present invention relates to an improved method for the cracking of aromatic hydrogen. [Background technology]

[0002] Polycyclic aromatic hydrocarbons (PAHs) are a class of toxic organic pollutants commonly found in aqueous phases. It is normally non-polar, hydrophobic, lipophilic, contains at least two benzene rings, and persists in the environment. These properties of polycyclic aromatic hydrocarbons can be used to purify polycyclic aromatic hydrocarbons in contaminated media. In most cases, biodegradation strategies are limited in their decomposition spectrum and are highly acidic. In addition, various environmental factors can reduce the efficiency of biological purification. Chemical purification techniques are expensive and prone to secondary pollution. In existing purification technologies for polycyclic aromatic hydrogen contaminated water areas, the decomposition efficiency of microorganisms is low and the decomposition spectrum is The torque is narrow, and the oxidation techniques are efficient and thorough, but expensive and highly toxic halogen-based. and nitro-based by-products. To improve the shortcomings of the technology, researchers have begun to focus on combining biological and oxidative purification. The researchers used a bacterial flora and calcium peroxide complex to measure the amount of polycyclic aromatic hydrocarbons (PAHs) in water. The technology to purify water uses free radicals generated in the decomposition of calcium peroxide and ferrous sulfate. However, there is a problem to be solved in that the growth of decomposition bacteria is inhibited by the fermentation process. Summary of the Invention

[0003] In order to solve the above problems, the present invention provides a method for the decomposition of polycyclic aromatic hydrocarbons using a decomposition bacterial consortium. A solution method is provided, which includes the following steps: S1: First, decomposition bacteria were added to the organic polluted water at a dosage of 1 to 11 wt%, and 150 rp The organic contaminated water was decomposed for 1 to 48 hours using a vibrator at 1000m. The decomposition flora is a proteobacterial genus Acinetobacter. netobacter, Stenotrophomonas , Comamonas genus bacteria were mixed in a weight ratio of 1:1:1. S2, then precomplex was added, and the amount of precomplex added in 1 liter of organically polluted water was 1.39 The decomposition flora and the precomplex form a decomposition flora complex system, and a vibrator The reaction was continued for a total of 72 hours to complete the decomposition. wherein the precomplex is composed of calcium peroxide and ferrous sulfate heptahydrate; The mass ratio of calcium to ferrous sulfate heptahydrate is 1-13.90:0.39-13.90. . Description: The above method is to determine the toxicity of calcium peroxide + ferrous sulfate to the decomposition flora in the decomposition system. It can solve the problem of bacterial degradation, and can degrade organic pollutants in water, especially polycyclic pollutants, which were previously degraded by conventional microbial decomposition techniques. It is good at solving the problems of low removal efficiency and narrow cracking spectrum of aromatic hydrogen. It has a solution effect, and utilizes the oxygen releasing and oxidizing properties of calcium peroxide, and decomposition technology to It has significantly improved the decomposition ability of polycyclic aromatic hydrogens, and decomposed calcium peroxide and ferrous sulfate. The system has a good decomposition effect, but the fluoride generated in the decomposition system of calcium peroxide + ferrous sulfate - Radicals not only remove pollutants, but also inhibit the growth of decomposing bacteria, and react with the above addition method. By controlling the time, the above effects can be effectively reduced, where calcium peroxide The system is not only complementary but also complementary, and compared to conventional microbial or oxidative purification, The method is more efficient, environmentally friendly and has high applicability. In one embodiment of the present invention, in S1, the decomposition reaction is carried out for 24 hours using a shaker at 150 rpm. Explanation: The reaction time is the equilibrium point at which the decomposition bacteria are retained while removing the contaminants. At the same time, the survival rate of decomposing bacteria is increased. In one aspect of the present invention, in S1, the pH value of the organic polluted water is 5 to 7, The aromatic ring hydrogens are phenanthrene and pyrene. In one embodiment of the present invention, in S2, 1 liter of organically contaminated water is treated with calcium peroxide. The amount added was 2.41 g, and the amount of ferrous sulfate heptahydrate added was 7.51 g. In one embodiment of the invention, the method for preparing the precomplex is as follows: S2-1, 25℃, 1~10μm activated carbon, magnesium oxide in a mass ratio of 3:0.5 Mix to obtain a carrier, S2-2, 15g: 1-2ml of hexadecylamine octane was applied to the surface of the carrier. Spraying acid carbonate, S2-3, the carrier and calcium peroxide are mixed in a ratio of 1 to 0.8:1 at a temperature of -15 to -10°C. The carrier sprayed with hexadecylamine octanoic acid carbonate and calcium peroxide were mixed in a weight ratio of The mixture was heated to 25°C and dried at 25°C for 1-2 hours to obtain the precomplex. S2-4, calcium peroxide and ferrous sulfate heptahydrate 1-13.90:0.39-13. The precomplex is mixed with ferrous sulfate heptahydrate in a weight ratio of 0.90 to form a precomplex. Description: The precomplex prepared by the above method is a mixture of organic pollutants, calcium peroxide and sulfuric acid. To provide a carrier for the reaction with ferrous heptahydrate, thereby improving the decomposition effect of contaminated water; Activated carbon and magnesium oxide are porous materials, and magnesium oxide catalyzes the decomposition of compounds. At the same time, the use of a carrier has the effect of promoting calcium peroxide and ferrous sulfate heptahydrate. This reduces the inhibitory effect of hydrates on the decomposition flora, further improving the decomposition ability of the system. It can be raised. In one embodiment of the present invention, in the step S2, the method of adding the precomplex is as follows: Divide calcium peroxide and ferrous sulfate heptahydrate into three equal parts, and first boil one third of them at 25°C. Add calcium peroxide, react for 5-15 minutes on a 150 rpm shaker, then shake for 3 minutes. Add 1 part of ferrous sulfate heptahydrate and react for 3-4 hours in a 120 rpm shaker. The temperature was lowered from 25°C to 5-10°C, and at the same time, one-third of the calcium peroxide and one-third of the sulfur were added. Add ferrous sulfate heptahydrate and react for 2-3 hours on a shaker at 180 rpm. Calcium oxide was added, and the reaction was continued for 20 to 25 minutes using a 150 rpm shaker. Add ferrous trihydrate and react to completion on a shaker at 130 rpm. Explanation: The above method of addition promotes the progress of the decomposition reaction and reduces the effect of the precomplex on the decomposition bacteria. This will reduce the amount of calcium peroxide and improve the decomposition effect of the calcium peroxide + ferrous sulfate decomposition system. The above stepwise addition method is a method of adding ferrous sulfate heptahydrate and calcium peroxide in combination. This effectively slows down the time it takes for the two to react together after the reaction to form a composite system. By controlling the time and temperature, the promotion effect on the decomposition reaction of free radicals is improved, and the decomposition The killing effect of the bacterial flora can be reduced. In one embodiment of the present invention, the proteobacteria Acinetobacter genus (A cinetobacter, Stenotrophomona s) and Comamonas are both habituated bacteria and are the only ones used for habituation. The target pollutant to be decomposed is utilized as the sole carbon source. In another preferred embodiment of the present invention, the decomposition bacterial consortium is screened from long-term contaminated soil. The long-term contaminated soil is a soil contaminated with polycyclic aromatic hydrocarbons that has been contaminated for more than two years. It is soil. Description: Screening and enrichment of soil long-term contaminated with polycyclic aromatic hydrocarbons The decomposition flora selected ensures that the flora used is more suitable for decomposing the relevant pollutants in the water body. The precomplex of decomposing bacteria and calcium peroxide (CP) was added to the polycyclic aromatic hydrogen contaminated water. After a certain period of reaction, the polycyclic aromatic hydrogen can be removed with high efficiency. The screening and enrichment method is as follows: S1-1, Pre-acclimation: Aromatic hydrocarbon contaminated soil, deionized water, and sodium sulfide in a mass ratio of 1:0.4:0.01~ The mixture was mixed at a ratio of 0.02 to obtain a mixture, and the mixture was mixed with phenanthrene in a mass ratio of 1:70 to 80. The mixture was added to a mixed nutrient solution containing arsenic and pyrene, and the mixture was cultured for 10 to 15 days for pre-acclimation. The sludge is dried until the moisture content is 30-40% to obtain pre-conditioned sludge. S1-2, Screening and enrichment: The pre-acclimated sludge obtained in step S1-1 is acclimated again, and the acclimation is performed as follows: The pre-conditioned sludge is added to the mixed nutrient solution in a mass ratio of 1:60 to 1:65 to obtain an conditioned liquid. The cells were cultured on a shaker at 0-200 rpm for 3-5 days per generation, and then subcultured 5-6 times. The phenanthrene content in the acclimated liquid was 2.2-fold higher than that in the pre-acclimated sludge. Incubate until it multiplies five-fold. S1-3, decomposition flora obtained: The conditioned liquid obtained in step S1-2 was inoculated at a mass fraction of 4 to 6% in 100 to 3,000 Inoculate inorganic salt medium containing 1000 mg / L phenanthrene / pyrene at 180-200 rpm. The mixture is then cultured for 24 to 64 hours using a shaker to obtain a decomposition flora. Description: The decomposition bacterial consortium enriched and acclimatized and screened by the above method was found to be capable of decomposing polycyclic aromatic hydrogens. It has a good decomposition effect on bacteria, and two acclimation steps with mixed nutrient solution promote the growth of bacteria. The bacterial flora can be enriched for efficient biphenyl degradation, and the bacterial flora can be enriched for efficient biphenyl decomposition. The diversity and polycyclic aromatic hydrogen cracking rate can be improved. The composition of the mixed nutrient solution described in S1-1 and S1-2 was yeast extract 1 g / L, peptone 5 g / L, and ethanol. g / L, urea 1.6g / L, K2HPO48g / L, deionized water 1L, phenanthrene 4 50 mg / L and pyrene 450 mg / L, pH 7.0±0.5, The composition of the salt medium was NH4Cl 1g / L, K2HPO4 1g / L, and MgSO4 0.2g / L. , FeSO4·7H2O0.02g / L, NaCl1g / L, (NH4)2SO43g / L , 0.01g / LCaCl2, MoO34mg / L, ZnSO4·5H2O28mg / L , CuSO4·5H2O0.02mg / L, H3BO34mg / L, MnSO4·5H2 O4 mg / L, CoCl2·6H2O4 mg / L, and pH 7.0 ± 0.5. The method of the present invention is to determine the toxicity of calcium peroxide and ferrous sulfate to the decomposition flora in the decomposition system. This can solve the problems of conventional microbial decomposition technology, particularly in the case of polycyclic organic pollutants in water. This is a good solution to the above problems of low removal efficiency of aromatic hydrogen and narrow decomposition spectrum. It has a decisive effect on the water by decomposition technology through the oxygen releasing and oxidizing properties of calcium peroxide. It belongs to the field of contaminated water purification, and is superior to conventional microbial purification by utilizing the decomposition ability of polycyclic aromatic hydrogen in water. Compared with chemical or oxidation purification, the method is more efficient, environmentally friendly and has high applicability. [Brief description of the drawings]

[0004] [Figure 1] 1 shows the composition of the decomposition bacterial flora screened and concentrated in Example 20. DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS

[0005] In order to better reflect the advantages of the present invention, the present invention will be more fully described below with reference to specific embodiments. This will be explained in more detail. Example 1 The method for decomposing polycyclic aromatic hydrocarbons using a decomposition bacterial consortium complex includes the following steps: S1: First, add 5 wt% of the decomposition bacteria to the organic polluted water and shake it at 150 rpm. The decomposition reaction was allowed to proceed for 24 hours. Here, the pH value of the organic polluted water is 6, and the organic polluted water is decomposed by polycyclic aromatic hydrogen. The polycyclic aromatic hydrogen is phenanthrene or pyrene, and the polycyclic aromatic hydrogen is a water body polluted with The decomposition flora is composed of the Proteobacteria Acinetobacter genus. , Stenotrophomonas, Comamonas amonas) in a 1:1:1 weight ratio. S2, then precomplex was added, and the amount of precomplex added in 1 liter of organically polluted water was 9.92 g, the decomposition flora and the precomplex form a decomposition flora complex, and the total time is The reaction was continued for 72 hours until the decomposition was complete. wherein the precomplex is composed of calcium peroxide and ferrous sulfate heptahydrate; The mass ratio of calcium to ferrous sulfate heptahydrate is 2.41:7.51.

[0006] Example 2 This example differs from Example 1 in the following points: In S1, the amount of decomposition flora added was 100%. It is 1 wt% of water. Example 3 This example differs from Example 1 in the following points: In S1, the amount of decomposition flora added was 100%. It is 11 wt% of water. Example 4 This embodiment differs from the first embodiment in the following respects: In S1, the vibration was performed for 1 h using a 150 rpm vibrator. The decomposition reaction is carried out. Example 5 This embodiment differs from the first embodiment in the following respects: In S1, the vibration was performed at 48 rpm with a 150 rpm vibrator. h Decomposition reaction. Example 6 This embodiment differs from embodiment 1 in the following points: In S1, the pH value of the organic polluted water is 5; do. Example 7 This embodiment differs from embodiment 1 in the following points: In S1, the pH value of the organic polluted water is 7. do. Example 8 This embodiment differs from the embodiment 1 in the following points: In S2, 1 liter of organic polluted water The amount of precomplex added was 1.39 g, where calcium peroxide was 1 g and dibasic sulfate was 1 g. Ferrous iron heptahydrate is 0.39 g. Example 9 This embodiment differs from the embodiment 1 in the following points: In S2, 1 liter of organic polluted water The amount of precomplex added was 27.80 g, where calcium peroxide was 13.9 g. , ferrous sulfate heptahydrate is 13.9 g. Example 10 This embodiment differs from Example 1 in the following respects: the components of the precomplex were improved, and the improved precomplex The complex contains two or more components, calcium peroxide and ferrous sulfate heptahydrate, and the improved precomplex The preparation method is as follows: S2-1, 25℃, 1~10μm activated carbon, magnesium oxide in a mass ratio of 3:0.5 Mix to obtain a carrier, S2-2, hexadecylamine octanoic acid carbon dioxide was applied to the surface of the carrier at a ratio of 15 g:1 ml. Spray the salt, S2-3, the carrier and calcium peroxide were mixed in a hexadecane in a weight ratio of 1:1 at a temperature of -15°C. The carrier sprayed with Silamine octanoic acid carbonate was mixed with calcium peroxide and heated to 25°C. The mixture was heated and dried at 25°C for 1 h to obtain the precomplex. S2-4: calcium peroxide and ferrous sulfate heptahydrate in a mass ratio of 1:0.39, The body is mixed with ferrous sulfate heptahydrate to form a precomplex. Example 11 This embodiment differs from Example 10 in the following respects: in S2-2, the carrier and hexadecyla The ratio of minoctanoic acid carbonate is 15g:2ml. Example 12 This embodiment differs from Example 10 in the following respects: in S2-2, the carrier and hexadecyla The ratio of minoctanoic acid carbonate is 15g:1.8ml. Example 13 This embodiment differs from Example 10 in the following respects: In S2-3, the temperature is -12°C, and the The carrier and calcium peroxide were mixed in a weight ratio of 0.9:1 with hexadecylamine octanoic acid carbonate. The sprayed carrier and calcium peroxide were mixed, heated to 25°C, and dried at 25°C for 1.5 h. to obtain the precomplex. Example 14 This embodiment differs from Example 10 in the following respects: In S2-3, the temperature is -10°C, and the The carrier and calcium peroxide were mixed in a weight ratio of 0.8:1 with hexadecylamine octanoic acid carbonate. The sprayed carrier and calcium peroxide were mixed, heated to 25°C, and dried at 25°C for 2 hours. Obtain the precomplex. Example 15 This example differs from Example 10 in the following respects: In S2-4, calcium peroxide and sulfur are The precomplex was mixed with ferrous sulfate heptahydrate in a mass ratio of 3:1 to obtain a precomplex. Form a coalescence. Example 16 This example differs from Example 10 in the following respects: In S2-4, calcium peroxide and sulfur are The precomplex was mixed with ferrous sulfate heptahydrate in a mass ratio of 1:1 to obtain a precomplex. Form a coalescence. Example 17 This embodiment differs from Example 1 in the following respects. In the above S2, the method of adding the precomplex is as follows: The formula is as follows: Calcium peroxide and ferrous sulfate heptahydrate are each divided into 3 equal parts. Add 1 / 3 of calcium peroxide at 5℃ and react for 10 min on a 150 rpm shaker. Then, one-third of ferrous sulfate heptahydrate was added and the mixture was shaken at 120 rpm for 3.5 h. After that, the temperature was lowered from 25°C to 8°C, and at the same time, 1 / 3 of calcium peroxide and 3 / 4 of the mixture were added. Add 1 part of ferrous sulfate heptahydrate and react for 2.5 hours in a 180 rpm shaker. The remaining calcium peroxide was added, and the mixture was reacted for 23 minutes on a 150 rpm shaker. Add ferrous sulfate heptahydrate and react to completion on a shaker at 130 rpm. Example 18 This embodiment differs from Example 17 in the following respects. In the above S2, the method of adding the precomplex is The results are as follows: Calcium peroxide and ferrous sulfate heptahydrate were each divided into three equal parts. At 25℃, add 1 / 3 of calcium peroxide and react for 5 minutes on a 150 rpm shaker. Then, one-third of the ferrous sulfate heptahydrate was added and reacted for 3 hours on a shaker at 120 rpm. Then, the temperature was lowered from 25°C to 5°C, and at the same time, one-third of the calcium peroxide and one-third of the of ferrous sulfate heptahydrate was added, and the mixture was reacted for 2 hours in a 180 rpm shaker. Add calcium oxide, react for 20 minutes with a 150 rpm shaker, and remove the remaining dilute sulfuric acid. Add iron heptahydrate and react to completion on a shaker at 130 rpm. Example 19 This embodiment differs from Example 17 in the following respects. In the above S2, the method of adding the precomplex is The results are as follows: Calcium peroxide and ferrous sulfate heptahydrate were each divided into three equal parts. At 25°C, add 1 / 3 of calcium peroxide and shake for 15 min at 150 rpm. Then, one-third of ferrous sulfate heptahydrate was added and the mixture was reacted for 4 hours on a 120 rpm shaker. After that, the temperature was lowered from 25°C to 10°C, and at the same time, 1 / 3 of calcium peroxide and 3 / 4 of the mixture were added. Add 1 part of ferrous sulfate heptahydrate, react for 3 hours in a 180 rpm shaker, and then add the remaining of calcium peroxide was added, and the mixture was reacted for 25 minutes on a shaker at 150 rpm. The remaining sulfuric acid was Add ferrous heptahydrate and react to completion on a shaker at 130 rpm. Example 20 This embodiment differs from Example 1 in the following respects. The screening and enrichment of the decomposition bacterial flora was carried out as follows. As follows: S1-1, Pre-acclimation: Polycyclic aromatic hydrocarbon-contaminated soil for 2 years, deionized water, sodium sulfide in a mass ratio of 1:0 The mixture was mixed in a ratio of 1:75 by mass with phenanthroline. The mixture was added to a mixed nutrient solution containing trene and pyrene, and the mixture was cultured for 12 days for pre-acclimation. The sludge was dried until the volume reached 35% to obtain a preconditioned sludge. S1-2, Screening and enrichment: The pre-acclimated sludge obtained in step S1-1 is acclimated again, and the acclimation is performed as follows: The pre-conditioned sludge was added to the mixed nutrient solution in a mass ratio of 1:62 to obtain a conditioned liquid, and 190 rp The cells were cultured at 1000 rpm on a shaker at 300 rpm for 4 days at each generation, and subcultured five times. The phenanthrene content was then increased to three times that of the pre-conditioned sludge, and the culture was continued until the phenanthrene content was three times that of the pre-conditioned sludge. The composition of the mixed nutrient solution described in steps S1-1 and S1-2 is yeast extract 1 g / L, pepto 5g / L, urea 1.6g / L, K2HPO48g / L, deionized water 1L, phenanthrene The concentrations of phenylalanine and pyrene were 450 mg / L and 450 mg / L, respectively, and the pH was 7.0 ± 0.5. S1-3, decomposition flora obtained: The conditioned liquid obtained in step S1-2 was inoculated with 2000 mg / L phenotype at a mass fraction of 5%. Inoculate into inorganic salt medium containing nanthrene / pyrene and culture for 50 h on a shaker at 190 rpm. The decomposition bacterial flora was obtained, and the results showed that the decomposition bacterial flora was Acinetobacter, St Contains three major bacteria: enotrophomonas, Comamonas, and decomposition bacteria The composition of the plexus is shown in FIG. The composition of the mineral salt medium was NH4Cl 1g / L, K2HPO4 1g / L, MgSO4 0. 2g / L, FeSO4·7H2O0.02g / L, NaCl1g / L, (NH4)2SO4 3g / L, 0.01g / LCaCl2, MoO34mg / L, ZnSO4·5H2O28 mg / L, CuSO4·5H2O0.02mg / L, H3BO34mg / L, MnSO4 ·5H2O 4 mg / L, CoCl2·6H2O 4 mg / L, and pH 7.0. Example 21 This embodiment differs from Example 20 in the following respects: in step S1-1, aromatic hydrocarbons are The contaminated soil, deionized water, and sodium sulfide were mixed in a mass ratio of 1:0.4:0.01. The mixture was mixed with a mixture containing phenanthrene and pyrene in a mass ratio of 1:70. The mixture was added to a nutrient solution and pre-cultured for 10 days. After that, it was dried until the water content was 30%. A pre-conditioned sludge is obtained. Example 22 This embodiment differs from Example 20 in the following respects: in step S1-1, aromatic hydrocarbons are The contaminated soil, deionized water, and sodium sulfide were mixed in a mass ratio of 1:0.4:0.02. The mixture was mixed with a mixture containing phenanthrene and pyrene in a mass ratio of 1:80. The mixture was added to a nutrient solution and pre-cultured for 15 days. It was then dried until the water content reached 40%. A pre-conditioned sludge is obtained. Example 23 This embodiment differs from Example 20 in the following respects, in that the culture parameters in step S1-2 are different. The pre-conditioned sludge was added to the mixed nutrient solution in a mass ratio of 1:65 to obtain an conditioned liquid. The cells were cultured in a PM shaker, cultured for 3 days at each generation, and subtype-expanded five times. The incubation is continued until the threne content is 4.5 times that of the phenanthrene content in the preconditioned sludge. . Example 24 This embodiment differs from Example 20 in the following respects: the culture parameters in step S1-2 are different. The pre-conditioned sludge was added to the mixed nutrient solution in a mass ratio of 1:60 to obtain an conditioned liquid. The culture was performed on a shaker at 400 rpm, with 5 d of culture per generation, and six subtypes were grown. The culture was continued until the phenanthrene content was 2.0 times that of the pre-conditioned sludge. do. Example 25 This embodiment differs from Example 20 in the following respects: the culture parameters in step S1-3 are different. The conditioned liquid obtained in step S1-2 was inoculated at a mass fraction of 4% to 3000 mg / Inoculate the inorganic salt medium containing L-phenanthrene / pyrene and incubate for 24 h on a shaker at 200 rpm. h Cultivate to obtain a decomposition flora. Example 26 This embodiment differs from Example 20 in the following respects: the culture parameters in step S1-3 are different. The conditioned liquid obtained in step S1-2 was inoculated at a mass fraction of 6% to give a concentration of 100 mg / L. Inoculate the medium containing phenanthrene / pyrene and incubate for 64 h on a shaker at 180 rpm. The decomposition bacteria are obtained by culturing. Experimental Example 1. Examination of the decomposition efficiency of the present invention against polycyclic aromatic hydrogen in organically contaminated water; Here, the decomposition rate of polycyclic aromatic hydrogen in water is calculated by the following formula: JPEG0007620786000002.jpg151471, Effects of the amount of decomposition bacteria and precomplex added on the decomposition efficiency of polycyclic aromatic hydrogens in organic polluted water Research, Comparative Example 1: Comparative Example 1 differs from Example 1 in the following respects: , without adding ferrous sulfate heptahydrate, only 5 wt% of the decomposition bacteria flora was added to decompose the material. Comparative Example 2: Comparative Example 2 differs from Example 1 in the following respects: decomposition bacteria were added to the organic polluted water. First, 2 g of calcium peroxide and 8.34 g of ferrous sulfate heptahydrate were added to decompose the solution. Table 1. Effect of the amount of decomposition bacteria added on the decomposition efficiency of polycyclic aromatic hydrogens TIFF0007620786000003.tif85159 As can be seen from Table 1, the results of comparing Example 1, Comparative Example 1, and Comparative Example 2 are as follows: Compared with the single purification technology of the microbial decomposition and CP system used alone in Example 2, In the composite technology of Example 1, the decomposition efficiency of polycyclic aromatic hydrogen in contaminated water is 29.97 to 59.5 1%, which indicates that the method of Example 1 provides the advantage of being system-compatible. Therefore, the method of Example 1 is more preferred. As a result of comparing Examples 1, 2, and 3, when the amount of decomposition flora added was 5 wt% The decomposition effect of polycyclic aromatic hydrogen is the best, because the decomposition bacteria is low when the amount of decomposition bacteria is low. If the amount of decomposition bacteria added is insufficient, the decomposition effect is low, and if the amount of decomposition bacteria added is further increased, the decomposition effect will There is no corresponding improvement, and therefore, from an economic point of view, the parameters of Example 1 are More preferred. 2. The separation of polycyclic aromatic hydrocarbons in organic polluted water by the addition of pre-complex and decomposition bacterial flora during treatment Examining the impact on solution efficiency, Comparative Example 3: This is different from Example 1 in the following respects: First, the precomplex was added and reacted for 24 hours. The decomposition bacteria was added and allowed to react for a total of 72 hours to complete the decomposition. Table 2. Effect of the decomposition bacterial consortium and precomplex methods on the decomposition efficiency of polycyclic aromatic hydrocarbons. TIFF0007620786000004.tif85160 As can be seen from Table 2, as a result of comparing Example 1 and Comparative Example 3, the addition order of Example 1 was more It is preferable that the decomposition order has a large effect on the decomposition of aromatic hydrocarbons, and Example 1 is more preferable. Ku, As a result of comparing Examples 1, 17, 18, and 19, the products prepared by the methods of Examples 17 to 19 were The composite system has a good decomposition effect of pyrene and phenanthrene, which is shown in Examples 17 to 19. By using the carrier, the decomposition effect of polluted water is improved, and activated carbon, magnesium oxide, The porous material has the effect of accelerating and promoting decomposition, so it has a strong decomposition ability for composite systems. Therefore, Example 19 is more preferable. 3. Investigating the effect of the pH value of organic polluted water on the decomposition efficiency of polycyclic aromatic hydrocarbons; Comparative Example 4: The following points are different from Example 1. The pH value of the organic polluted water is 3. Comparative Example 5: The following points are different from Example 1. The pH value of the organic polluted water is 11. Table 3. Effect of pH value of organically contaminated water on the decomposition efficiency of polycyclic aromatic hydrogens TIFF0007620786000005.tif83158 As can be seen from Table 3, Example 1, Example 6 and Example 7, and Comparative Example 4 and Comparative Example 5 were compared. As a result, the pH in Examples 1, 6 and 7, i.e., the pH value is 5 to 7. The decomposition efficiency of the precomplex was the best. As a result of comparing Example 4, Comparative Example 4 and Comparative Example 5, If the value is too low or too high, the decomposition efficiency of the system will be poor, but it is still possible to obtain a certain amount of polycyclic aromatic hydrocarbons. maintains resolving power. 4. Investigating the effect of the components of the decomposition microbial flora on the decomposition efficiency of polycyclic aromatic hydrocarbons in organically polluted water; Comparative Example 6: The decomposition bacteria flora was an existing PAHs decomposition strain, and Quoted from "Advances in Applied Research and Its Applications" Table 4. Effect of components of the decomposition flora on the decomposition efficiency of polycyclic aromatic hydrocarbons TIFF0007620786000006.tif60158 As can be seen from Table 4, the results of comparing Example 1 and Comparative Example 6 show that the decomposition bacteria used in Example 1 The decomposition effect of the flora is more preferable. As a result of comparing Example 1 and Example 20, In addition, the decomposition effect of polycyclic aromatic hydrocarbons in water bodies using the acclimatized and concentrated decomposition bacterial flora is also good. 2. Investigation of the anti-interference performance of the decomposition process in Example 1 Two types of dissolved organic matter, fulvic acid and humic acid, were used to examine the decomposition ability of precomplexes. , Comparative Example 7: Different from Example 1 in the following respects, 100 mg / L fulvic acid was added to the organically polluted water. Then, decompose it. Comparative Example 8: The following points differ from Example 1, and 250 mg / L fulvic acid was added to the organically polluted water. Then, decompose it. Comparative Example 9: The following points are different from Example 1, and 100 mg / L humic acid was added to the organically polluted water. Then, decompose it. Comparative Example 10: This is different from Example 1 in the following respects: 250 mg / L humic acid was added to the organic polluted water. Add and decompose, Table 5. Anti-interference performance of decomposition process TIFF0007620786000007.tif79160 As can be seen from Table 5, the results of comparing Example 1, Comparative Example 7, and Comparative Example 8 show that fulvic acid is effective in preventing the formation of inflammatory bowels. The efficiency of decomposition of polycyclic aromatic hydrogen by the reaction is reduced. As a result, it was found that humic acid also reduced the decomposition efficiency of polycyclic aromatic hydrocarbons by the system, but the reduction in both The degree of degradation is limited, and the precomplex still maintains some degradative capacity.

Claims

1. S1: First, decomposition bacteria were added to the organic polluted water at a dosage of 1 to 11 wt%, and the mixture was stirred for 150 rpm. The organic polluted water was decomposed by a vibration exciter of 1000m for 1 to 48 hours. The decomposition flora is a proteobacterial genus Acinetobacter (Ac. inetobacter, Stenotrophomonas ), Comamonas genus (Comamonas) three kinds of bacteria are mixed in a weight ratio of 1:1:

1. Tep and S2, then precomplex was added, and the amount of precomplex added per liter of organically contaminated water was 1.39 The decomposition bacterial flora and the precomplex form a decomposition bacterial flora complex system, and a vibrator and continuously reacting for a total time of 72 h to complete the decomposition. wherein the precomplex is composed of calcium peroxide and ferrous sulfate heptahydrate; The mass ratio of calcium to ferrous sulfate heptahydrate is 1-13.90:0.39-13.

90. 、 In S1, the pH value of the organic polluted water is 5 to 7, and the polycyclic aromatic hydrocarbons are phenyl. nanthrene, pyrene, A method for decomposing polycyclic aromatic hydrocarbons using a complex decomposition bacterial consortium, comprising:

2. In step S1, the decomposition reaction is carried out for 24 hours using a vibrator at 150 rpm.

1. The method according to claim 1.

3. In S2, the amount of calcium peroxide added to 1 liter of organically contaminated water is 2.41 g.

2. The method according to claim 1, wherein the amount of ferrous sulfate heptahydrate added is 7.51 g. How to.

4. The method for preparing the precomplex is as follows: S2-1, 25°C, 1-10 μm activated carbon, magnesium oxide in a mass ratio of 3:0.5 Mix to obtain a carrier, S2-2, 15 g: 1 to 2 ml of hexadecylamine octane was applied to the surface of the carrier. Spraying acid carbonate, S2-3: The carrier and calcium peroxide are mixed in a ratio of 1 to 0.8:1 at a temperature of -15 to -10°C. The carrier sprayed with hexadecylamine octanoic acid carbonate and calcium peroxide were mixed in a weight ratio of The mixture was heated to 25°C and dried at 25°C for 1-2 h to obtain the precomplex. S2-4, calcium peroxide and ferrous sulfate heptahydrate 1 to 13.90:0.39 to 13. 90% by weight of the precomposite and ferrous sulfate heptahydrate to form a precomposite; The method of claim 1, further comprising:

5. In the above S2, the method of adding the precomplex is as follows: calcium peroxide, sulfuric acid Ferrous heptahydrate was divided into three equal parts, and one-third of the calcium peroxide was added at 25°C. The mixture was then reacted for 5 to 15 minutes using a 150 rpm shaker, and then one-third of the ferrous sulfate heptahydrate was added. The mixture was then reacted for 3-4 hours with a 120 rpm shaker, and the temperature was then lowered from 25°C to 5-1°C. Reduce temperature to 0°C and add 1 / 3 calcium peroxide and 1 / 3 ferrous sulfate heptahydrate at the same time. The mixture was then reacted for 2-3 hours using a 180 rpm shaker, after which the remaining calcium peroxide was added. The mixture was then reacted for 20-25 min with a 150 rpm shaker, and the remaining ferrous sulfate heptahydrate was added.

2. The method according to claim 1, further comprising: subjecting the mixture to a shaking machine at 130 rpm until the reaction is complete. Law.

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