Specimen processing device and nucleic acid detection integrated machine equipped with the same

Abstract

To provide a specimen treatment device and a nucleic acid detection integrated machine comprising the same.SOLUTION: A specimen treatment device comprises: a frame body 10 having a sample treatment platform 11; an extraction mechanism 30 for extracting an analysis object; a gripping part 40 which is movably provided above the sample treatment platform; a shift member 50 movably provided on the sample treatment platform; and a liquid transfer part which is movably provided above the sample treatment platform. The shift member includes an extraction plate shift member 53 having a first shift position. The gripping part has a first gripping position and a second gripping position. When the extraction plate shift member is at the first shift position, the gripping part is movable between the first and second gripping positions. The extraction plate shift member further has a sample addition position. When the extraction plate shift member is at the sample addition position, the liquid transfer part further has a first liquid transfer position.SELECTED DRAWING: Figure 1

Description

Technical Field

[0001] The present invention relates to the technical field of biological detection, and specifically to a specimen processing device and a nucleic acid detection integrated machine equipped with the same.

Background Art

[0002] Currently, nucleic acid detection is widely used in fields such as clinical diagnosis, agricultural monitoring, and food safety. In particular, when a large-scale infectious disease occurs, nucleic acid detection can be used as the gold standard for disease confirmation. The currently commonly used nucleic acid detection method is PCR detection. The general principle of PCR detection is a process of replicating daughter-strand DNA complementary to the parent-strand DNA in vitro using the parent-strand DNA as a template and a specific primer as the elongation starting point under the catalytic action of DNA polymerase.

[0003] In related technologies, for a nucleic acid detection integrated machine to extract parent-strand DNA, its multiple modules need to cooperate with each other to complete. By transferring consumables between each module with a movable gripper, it is possible to extract parent-strand DNA from a sample using an extraction reagent.

[0004] However, the transfer of consumables between different modules needs to be realized by different grippers. Alternatively, it is necessary to make the operating area of the gripper large enough. Both of the above two solutions require a large amount of space, leading to an increase in the volume of the device.

Summary of the Invention

Problems to be Solved by the Invention

[0005] In order to solve the problem that the volume of the nucleic acid detection integrated machine in related technologies is large, the present invention provides a specimen processing device and a nucleic acid detection integrated machine equipped with the same.

Means for Solving the Problems

[0006] According to one aspect of the present invention, there is provided a specimen processing apparatus including: a frame having a sample processing platform; an extraction mechanism for extracting an analyte in a specimen; a gripping portion movably provided above the sample processing platform along a vertical direction and a first horizontal direction; a transfer member movably provided on the sample processing platform along a second horizontal direction perpendicular to the first horizontal direction; and a liquid transfer portion movably provided above the sample processing platform. The transfer member includes an extraction plate transfer member having a first transfer position that moves corresponding to a position below the gripping portion. The gripping portion has a first gripping position located above the extraction plate transfer member and a second gripping position located above the extraction mechanism. When the extraction plate transfer member is located at the first transfer position, the gripping portion can move between the first gripping position and the second gripping position so as to move the extraction plate between the extraction plate transfer member and the extraction mechanism. Among them, the extraction plate transfer member further has a sample addition position where it moves to a corresponding liquid transfer portion. When the extraction plate transfer member is located at the sample addition position, the liquid transfer portion further has a first liquid transfer position where it moves above the extraction plate transfer member.

[0007] Furthermore, the transfer member further includes an amplification plate transfer member. Both the extraction plate transfer member and the amplification plate transfer member move along the second horizontal direction. The amplification plate transfer member has a nucleic acid addition position flush with the extraction plate transfer member. The liquid transfer portion further has a second liquid transfer position corresponding to a position above the amplification plate transfer member. When the extraction plate transfer member is located at the sample addition position and the amplification plate transfer member is located at the nucleic acid addition position, the liquid transfer portion can switch between the first liquid transfer position and the second liquid transfer position.

[0008] Furthermore, the extraction mechanism includes an extraction member and a transfer member that is movably bored along a second horizontal direction in the extraction member. The transfer member has a transfer position corresponding to below the gripping portion and an extraction position located below the extraction member. When the extraction plate transfer member is located at the first transfer position and the transfer member is located at the transfer position, the gripping portion can move the extraction plate between the extraction plate transfer member and the transfer member.

[0009] Furthermore, the specimen processing apparatus further includes a film sealing mechanism for sealing the amplification plate. The film sealing mechanism includes a placement member and a hot press member for sealing the amplification plate. The hot press member is located above the placement member. The placement member is provided movably along a second horizontal direction on the sample processing platform. The placement member has an amplification plate placement position for placing the amplification plate. The amplification plate transfer member has a second transfer position located below the gripping portion. The gripping portion further has a third gripping position located above the placement member and a fourth gripping position located above the amplification plate transfer member. When the amplification plate transfer member is located at the second transfer position, the gripping portion can move between the third gripping position and the fourth gripping position so as to move the amplification plate from the amplification plate transfer member to the amplification plate placement position.

[0010] Furthermore, the placement member further has a lid placement position for placing the lid. When the gripping portion is located at the third gripping position, the placement member has a separation position where the lid placement position moves below the gripping portion and the gripping portion grips the lid, a release position where the amplification plate placement position moves below the gripping portion and the gripping portion releases the lid to the amplification plate, and a sealing position where the amplification plate placement position moves below the hot press member.

[0011] Furthermore, the film sealing mechanism includes a film sealing frame and a detection member. The film sealing frame is provided on the sample processing platform. Both the thermal pressing member and the detection member are fixedly installed on the film sealing frame. The placement member is provided on the film sealing frame so as to be movable along the second horizontal direction. The detection member can detect the placement state of the placement member.

[0012] Furthermore, the lid placement position has a first position limiting protrusion for limiting the position of the lid, and / or the amplification plate placement position has a second position limiting protrusion for limiting the position of the amplification plate.

[0013] Furthermore, the specimen processing apparatus further includes a consumable storage bin for placing the liquid transfer head. The liquid transfer unit has a load position where it moves above the consumable storage bin to load the liquid transfer head.

[0014] Furthermore, the liquid transfer unit includes a sample arm and a liquid transfer needle. The sample arm is provided on the frame body so as to be horizontally movable and is located above the sample processing platform. The liquid transfer needle is provided on the sample arm so as to be vertically movable.

[0015] Furthermore, the frame body further includes a first guide rail and a second guide rail located above the sample processing platform. The first guide rail extends in the first horizontal direction. The second guide rail extends in the second horizontal direction. The first guide rail is provided on the frame body so as to be movable along the extension direction of the second guide rail below the second guide rail. The sample arm is provided on the first guide rail so as to be movable along the extension direction of the first guide rail.

[0016] Furthermore, the gripping portion has a gripping frame and a gripper provided on the gripping frame so as to be vertically movable. The gripping frame is provided on the frame body so as to be movable along the first horizontal direction. The gripper has a first gripping position and a second gripping position.

[0017] According to another aspect of the present invention, there is provided a nucleic acid detection integrated machine including a reagent preparation device for preparing extraction reagents and amplification reagents, an amplification detection device for performing an analyte detection measurement on a measurement mixture to analyze a specimen, and the specimen processing device provided above.

[0018] Furthermore, the reagent preparation device, the specimen processing device, and the amplification detection device are isolated from each other. The reagent preparation device and the amplification detection device are located on both sides of the specimen processing device respectively. A first passage is provided between the reagent preparation device and the specimen processing device, and a second passage is provided between the specimen processing device and the amplification detection device. Both the first passage and the second passage have a blocked state and a conductive state, and / or the nucleic acid detection integrated machine further includes a transfer mechanism provided between the reagent preparation device and the specimen processing device. The transfer mechanism can move an extraction plate for preparing extraction reagents, an amplification plate for preparing amplification reagents, and a lid through the first passage to the specimen processing device. The gripping part of the specimen processing device can move the extraction plate to the extraction plate transfer member of the specimen processing device and move the amplification plate to the amplification plate transfer member of the specimen processing device.

Advantages of the Invention

[0019] If the technical means of the present invention are applied, the specimen processing apparatus includes a frame body, an extraction mechanism, a gripping part, a transfer member, and a liquid transfer part. When extracting an analyte in a specimen, the liquid transfer part is used to aspirate the specimen, and further, the extraction plate transfer member is moved to the sample position and the liquid transfer part is moved to the first liquid transfer position, so that the liquid transfer part discharges the specimen onto the extraction plate of the extraction plate transfer member. Further, the extraction plate transfer member is moved to the first transfer position, and the gripping part is moved to the first gripping position located above the extraction plate transfer member, and the gripping part is used to grip the extraction plate on the extraction plate transfer member. Further, the gripping part is moved to the second gripping position located above the extraction mechanism, and the extraction plate is discharged to the extraction mechanism, and further, the extraction mechanism is used to extract the analyte in the specimen. Since the extraction of the analyte is realized only by the movement of the gripping part along the vertical direction and the first horizontal direction, and the movement of the extraction plate transfer member along the second horizontal direction, the occupation of the space of the specimen processing apparatus can be significantly reduced, and further, the volume of the specimen processing apparatus can be reduced.

[0020] The drawings constituting a part of this application are for further understanding of the present invention, and the exemplary embodiments of the present invention and their descriptions are for interpreting the present invention and do not unduly limit the present invention.

Brief Description of the Drawings

[0021]

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Embodiments for Carrying Out the Invention

[0022] Hereinafter, the technical means of the embodiments of the present invention will be clearly and completely described with reference to the drawings in the embodiments of the present invention. It goes without saying that the embodiments described below are only some of the embodiments of the present invention, not all of them. The description of at least one exemplary embodiment below is actually only for the purpose of explanation and does not impose any limitation on the present invention and its application or use. All other embodiments obtained by those skilled in the art without creative labor based on the embodiments of the present invention belong to the protection scope of the present invention.

[0023] As shown in FIGS. 1 to 5, an embodiment of the present invention provides a specimen processing apparatus including a frame body 10, an extraction mechanism 30, a gripping portion 40, a transfer member 50, and a liquid transfer portion 60. The frame body 10 has a sample processing platform 11. The extraction mechanism 30 is for extracting an analyte in a specimen. The gripping portion 40 is provided above the sample processing platform 11 so as to be movable in the vertical direction and a first horizontal direction. The transfer member 50 is provided above the sample processing platform 11 so as to be movable along a second horizontal direction perpendicular to the first horizontal direction. The transfer member 50 includes an extraction plate transfer member 53 having a first transfer position that moves corresponding to below the gripping portion 40. The gripping portion 40 has a first gripping position located above the extraction plate transfer member 53 and a second gripping position located above the extraction mechanism 30. When the extraction plate transfer member 53 is located at the first transfer position, the gripping portion 40 can move between the first gripping position and the second gripping position so as to move the extraction plate between the extraction plate transfer member 53 and the extraction mechanism 30. The liquid transfer portion 60 is movably provided above the sample processing platform 11. Among them, the extraction plate transfer member 53 further has a sample addition position that moves to the corresponding liquid transfer portion 60. When the extraction plate transfer member 53 is located at the sample addition position, the liquid transfer portion 60 further has a first liquid transfer position that moves above the extraction plate transfer member 53.

[0024] When the technical means of the present invention are applied, the specimen processing device includes a frame body 10, an extraction mechanism 30, a gripping part 40, a transfer member 50, and a liquid transfer part 60. When extracting an analyte in a specimen, first, the liquid transfer part 60 is used to aspirate the specimen. Next, the extraction plate transfer member 53 is moved to the sample addition position, and the liquid transfer part 60 is moved to the first liquid transfer position, so that the liquid transfer part 60 discharges the specimen onto the extraction plate of the extraction plate transfer member 53. Further, the extraction plate transfer member 53 is moved to the first transfer position, and the gripping part 40 is moved to the first gripping position located above the extraction plate transfer member 53, and the gripping part 40 is used to grip the extraction plate on the extraction plate transfer member 53. Then, the gripping part 40 is moved to the second gripping position located above the extraction mechanism 30, and the extraction plate is discharged to the extraction mechanism 30. Further, the extraction mechanism 30 is used to extract the analyte in the specimen. Since the extraction of the analyte is realized only by the movement of the gripping part 40 along the vertical direction and the first horizontal direction, and the movement of the extraction plate transfer member 53 along the second horizontal direction, the occupation of the space of the specimen processing device can be significantly reduced, and further the volume of the specimen processing device can be reduced.

[0025] Note that the specimen may be transferred from outside the device to the sample processing platform 11 inside the device, or a specimen loading member 20 may be provided on the sample processing platform 11, and the specimen may be placed on the specimen loading member 20.

[0026] In this embodiment, the specimen loading member 20 includes a specimen bottle for accommodating the specimen and an opening mechanism. The specimen is accommodated in a specimen tube, the specimen tube is placed in the specimen bottle, and is opened by the opening mechanism. The opened specimen tube is conveyed below the liquid transfer part 60 so as to aspirate the specimen.

[0027] Note that the specimen includes one or more of a sample, a quality control material, or a calibration material that needs to be measured.

[0028] As shown in FIGS. 1 and 3, the extraction mechanism 30 includes an extraction member 31 and a transfer member 32 that is movably formed in the extraction member 31 along a second horizontal direction. The transfer member 32 has a transfer position corresponding to below the gripping portion 40 and an extraction position located below the extraction member 31. When the extraction plate transfer member 53 is located at the first transfer position and the transfer member 32 is located at the transfer position, the gripping portion 40 can move the extraction plate between the extraction plate transfer member 53 and the transfer member 32. By adopting the above structure, the transfer of the extraction plate between the extraction plate transfer member 53 and the extraction member 31 can be realized by using the transfer member 32, and it has the advantages of simple structure and easy realization.

[0029] In this embodiment, both the extraction plate transfer member 53 and the transfer member 32 can move along a second horizontal direction below the gripping portion 40. As shown in FIGS. 1 to 3, the transfer member 50 includes an amplification plate transfer member 54, and both the extraction plate transfer member 53 and the amplification plate transfer member 54 move along the second horizontal direction. The amplification plate transfer member 54 has a nucleic acid addition position flush with the extraction plate transfer member 53, and the liquid transfer portion 60 further has a second liquid transfer position corresponding to above the amplification plate transfer member 54. When the extraction plate transfer member 53 is located at the sample addition position and the amplification plate transfer member 54 is located at the nucleic acid addition position, the liquid transfer portion 60 can be switched between the first liquid transfer position and the second liquid transfer position. When the amplification plate transfer member 54 is located at the nucleic acid addition position and the extraction plate transfer member 53 is located at the sample addition position, the liquid transfer portion 60 can be used to inject the extracted nucleic acid from the extraction plate located on the extraction plate transfer member 53 into the amplification plate located on the amplification plate transfer member 54, and it has the advantages of compact structure and easy operation.

[0030] In this embodiment, the extraction plate transfer member 53 and the amplification plate transfer member 54 are provided to be parallel. On the sample processing platform 11, two parallel rails are provided, and both of the two rails extend along the second horizontal direction. The extraction plate transfer member 53 and the amplification plate transfer member 54 are respectively movably provided on the corresponding rails.

[0031] After the analyte is extracted from the specimen on the extraction plate by the extraction mechanism 30, the extraction plate is grasped by the grasping portion 40 and discharged to the extraction plate transfer member 53 located at the first transfer position. Further, the extraction plate transfer member 53 is moved to the sample addition position, and using the liquid transfer portion 60, it is moved to the first liquid transfer position to suck the analyte after extraction on the extraction plate. Then, by further moving the liquid transfer portion 60 to the second liquid transfer position located above the amplification plate transfer member 54 to inject the analyte into the amplification plate, the transfer of the analyte can be completed.

[0032] Among them, the amplification plate transfer member 54 further has a second transfer position that moves corresponding to the lower part of the grasping portion 40 so that the grasping portion 40 loads the amplification plate.

[0033] By the grasping portion 40 moving along the vertical direction and the first horizontal direction, the transfer of the extraction plate between the extraction plate transfer member 53 and the extraction mechanism 30 is realized. By the extraction plate transfer member 53, the amplification plate transfer member 54, and the transfer member 32 all transferring along the second horizontal direction, after the specimen is sucked by the liquid transfer portion 60, it can be transferred to the extraction member 31 to perform the extraction of the analyte, and the transferred analyte after extraction can be transferred from the extraction plate to the amplification plate, which can make the arrangement of each member more reasonable and compact, and reduce the volume of the device.

[0034] As shown in FIGS. 1 and 4, the specimen processing apparatus further includes a film sealing mechanism 70 for sealing the amplification plate. The film sealing mechanism 70 includes a placement member 72 and a hot press member 71 for sealing the amplification plate. The hot press member 71 is located above the placement member 72. The placement member 72 is provided movably along a second horizontal direction on the sample processing platform 11. The placement member 72 has an amplification plate placement position 722 for placing the amplification plate. The amplification plate placement position 722 has a third transfer position that moves corresponding to below the gripping portion 40. The amplification plate transfer member 54 has a second transfer position located below the gripping portion 40. The gripping portion 40 further has a third gripping position located above the placement member 72 and a fourth gripping position located above the amplification plate transfer member 54. When the amplification plate transfer member 54 is located at the second transfer position and the amplification plate placement position 722 is located at the third transfer position, the gripping portion 40 can move between the third gripping position and the fourth gripping position so as to move the amplification plate from the amplification plate transfer member 54 to the amplification plate placement position 722. By adopting the above structure, the amplification plate can be placed using the placement member 72, the amplification plate on which the analyte is placed can be moved to the placement member 72 using the gripping portion 40, and the sealing of the amplification plate can be completed using the hot press member 71.

[0035] And the transfer of both the extraction plate and the amplification plate is performed by the gripping portion 40. In the transfer process of the extraction plate and the amplification plate, since the extraction plate and the amplification plate have a common movement path, it is advantageous for further reducing the occupation of space and making the structure of the specimen processing apparatus more compact.

[0036] As shown in FIG. 4, the placement member 72 further has a lid placement position 721 for placing the lid. When the gripping portion 40 is located at the third gripping position, the placement member 72 has a separation position where the lid placement position 721 moves below the gripping portion 40 and the gripping portion 40 grips the lid, a release position (corresponding to the above-mentioned third transfer position) where the amplification plate placement position 722 moves below the gripping portion 40 and the gripping portion 40 releases the lid onto the amplification plate, and a sealing position where the amplification plate placement position 722 moves below the hot pressing member 71. Within the movement range of the gripping portion 40, by the movement of the placement member 72 along the second horizontal direction and the cooperation with the gripping portion 40, the sealing of the lid and the amplification plate can be realized, and there is an advantage that the structure is compact.

[0037] Specifically, when the gripping portion is located at the fourth gripping position, the placement member 72 can be moved to the separation position, and further, the gripping portion 40 can be used to grip the lid and detach it from the lid placement position 721. Furthermore, by moving the placement member 72 along the second horizontal direction to the release position, the amplification plate placement position 722 is moved below the gripping portion 40 to release the lid onto the amplification plate. Furthermore, the placement member 72 is moved to the sealing position, and the hot pressing member 71 is used to seal the lid and the amplification plate.

[0038] As shown in FIG. 4, the film sealing mechanism 70 includes a film sealing frame 73 and a detection member. The film sealing frame 73 is provided on the sample processing platform 11, and both the hot pressing member 71 and the detection member are fixedly installed on the film sealing frame 73. The placement member 72 is provided on the film sealing frame 73 so as to be movable along the second horizontal direction, and the detection member can detect the placement state of the placement member 72. The detection member can be used to detect whether an amplification plate, a lid, or an amplified plate and a lid after sealing are provided on the placement member 72.

[0039] In this embodiment, the lid placement position 721 has a first position limiting protrusion for limiting the position of the lid, and the amplification plate placement position 722 has a second position limiting protrusion for limiting the position of the amplification plate. By using the first position limiting protrusion, the position of the lid can be limited, and the lid can be made more stable at the lid placement position 721. By using the second position limiting protrusion, the position of the amplification plate can be limited, and the amplification plate can be made more stable at the amplification plate placement position 722.

[0040] As shown in FIG. 2, the specimen processing apparatus further includes a consumable storage bin 80 for placing a liquid transfer head, and the liquid transfer unit 60 has a load position where it moves above the consumable storage bin 80 to load the liquid transfer head. The liquid transfer head can be stored using the consumable storage bin 80. Further, the liquid transfer unit 60 can be used to load the liquid transfer head, and the liquid transfer head can be replaced before sucking the specimen or the analyte so as to prevent contamination between the extraction reagent, the amplification reagent, the specimen, and the analyte.

[0041] Specifically, before the liquid transfer unit 60 sucks the specimen in the specimen tube, it is necessary to move the liquid transfer unit 60 above the consumable storage bin 80 to load the liquid transfer head. Further, the specimen is sucked using the liquid transfer head. The suction process of the analyte is similar, so the description is omitted here.

[0042] As shown in FIG. 1, the liquid transfer unit 60 includes a sample arm 61 and a liquid transfer needle 62. The sample arm 61 is provided on the frame body 10 so as to be horizontally movable and is located above the sample processing platform 11. The liquid transfer needle 62 is provided on the sample arm 61 so as to be vertically movable. By adopting the liquid transfer unit 60 having the above structure, the three-axis movement of the liquid transfer needle 62 can be realized by the cooperation of the sample arm 61 and the liquid transfer needle 62.

[0043] In this embodiment, the liquid transfer head can be loaded using the liquid transfer needle 62. Of course, after each liquid transfer operation is completed, the liquid transfer needle 62 can be cleaned, or a plurality of liquid transfer needles 62 can be provided on the sample arm 61, and different liquid transfer needles 62 can be used for each operation to prevent contamination.

[0044] As shown in FIGS. 1 and 5, the frame body 10 further includes a first guide rail 12 and a second guide rail 13 located above the sample processing platform 11. The first guide rail 12 extends in the first horizontal direction, and the second guide rail 13 extends in the second horizontal direction. The first guide rail 12 is movably provided above the second guide rail 13 along the extending direction of the second guide rail 13. The sample arm 61 is movably provided on the first guide rail 12 along the extending direction of the first guide rail 12. By adopting the first guide rail 12 and the second guide rail 13, the horizontal movement of the sample arm 61 can be realized, and it has the advantage of simple structure.

[0045] As shown in FIG. 5, the gripping part 40 has a gripping frame and a gripper movably provided in the vertical direction on the gripping frame. The gripping frame is movably provided on the frame body 10 along the first horizontal direction. The gripper has a first gripping position and a second gripping position. By adopting the gripping part 40 with the above structure, the biaxial movement of the gripper in the vertical plane can be realized using the gripping frame, and it has the advantage of simple structure.

[0046] The following describes the usage process of the specimen processing apparatus in combination. When extracting the analyte in the specimen, first, the specimen is aspirated using the liquid transfer needle 62 in the liquid transfer unit 60. Next, the liquid transfer needle 62 is moved to the first liquid transfer position located above the extraction plate transfer member 53, and the extraction plate transfer member 53 is moved to the sample addition position, and the specimen is discharged onto the extraction plate of the extraction plate transfer member 53 using the liquid transfer needle 62. Further, the extraction plate transfer member 53 is moved to the first transfer position located below the gripping unit 40, and the gripping unit 40 is moved to the first gripping position, and the extraction plate on the extraction plate transfer member 53 is gripped using the gripping unit 40. Further, the gripping unit 40 is moved to the second gripping position and positioned above the transfer member 32 at the transfer position, and the extraction plate is discharged onto the transfer member 32, and the transfer member 32 is moved to the extraction position located on the extraction member 31, and the analyte in the specimen is extracted using the extraction member 31. After extracting the analyte, the transfer member 32 is moved from the extraction position to the transfer position, and the extraction plate is gripped using the gripping unit 40 and discharged onto the extraction plate transfer member 53 at the first transfer position. Further, the extraction plate transfer member 53 is moved to the sample addition position, the liquid transfer needle 62 is moved to the first liquid transfer position, and the analyte after extraction on the extraction plate is aspirated using the liquid transfer needle 62. Further, the liquid transfer needle 62 is moved to the second liquid transfer position and positioned above the amplification plate transfer member 54 at the nucleic acid addition position, and the analyte is discharged onto the amplification plate. Further, the amplification plate transfer member 54 is moved to the second transfer position, and the gripping unit 40 is moved to the fourth gripping position, and the amplification plate is gripped using the gripping unit 40. Further, the gripping unit 40 is moved to the third gripping position located above the placement member 72, and the amplification plate is discharged onto the amplification plate placement position 722. And the placement member 72 is moved to the separation position, the lid is gripped using the gripping unit 40. Further, the placement member 72 is moved to the discharge position, the lid is discharged onto the amplification plate. Further, the placement member 72 is moved to the sealing position, and the lid and the amplification plate are sealed using the thermal press member 71.Therefore, the specimen processing device can realize the movement of the extraction plate, amplification plate, and lid along the same path, and the combination of the amplification plate and the lid, has the feature of simple structure, and reduces the volume of the specimen processing device.

[0047] Another embodiment of the present invention provides a nucleic acid detection integrated machine including a reagent preparation device, a specimen processing device, and an amplification detection device. The reagent preparation device is for preparing an extraction reagent and an amplification reagent. The amplification detection device is for analyzing and detecting a measurement mixture. The specimen processing device is the specimen processing device provided above. By applying the nucleic acid detection integrated machine, extraction of the analyte can be realized only by the movement of the grasping part 40 along the vertical direction and the first horizontal direction, and the movement of the transfer member 50 along the second horizontal direction. Therefore, the occupation of the space of the specimen processing device can be greatly reduced, and the volume of the specimen processing device can be reduced.

[0048] In this embodiment, the reagent preparation device, the specimen processing device, and the amplification detection device are isolated from each other. The reagent preparation device and the amplification detection device are respectively located on both sides of the specimen processing device. A first passage is provided between the reagent preparation device and the specimen processing device, and a second passage is provided between the specimen processing device and the amplification detection device. Both the first passage and the second passage have a blocked state and a conductive state. By adopting the above structure, isolation between each device module can be realized by using the blocking of the first passage and the second passage, and mutual contamination can be avoided. By using the conduction of the first passage and the second passage, the transfer of consumables between each device module can be realized.

[0049] Among them, the nucleic acid detection integrated machine further includes a transfer mechanism provided between the reagent preparation device and the specimen processing device. The transfer mechanism can move an extraction plate for preparing an extraction reagent, an amplification plate for preparing an amplification reagent, and a lid through a first passage to the specimen processing device. The gripping part 40 of the specimen processing device can move the extraction plate to the extraction plate transfer member 53 of the specimen processing device and move the amplification plate to the amplification plate transfer member 54 of the specimen processing device. By using the transfer mechanism, the extraction plate on which the preparation of the extraction reagent is completed and the amplification plate on which the preparation of the amplification reagent is completed in the reagent preparation device can be moved to the specimen processing device to perform the extraction of the specimen.

[0050] In this embodiment, the extraction plate, the amplification plate, and the lid are all transferred from the reagent preparation device to the specimen extraction device by the transfer mechanism. The gripping part 40 is used to transfer the extraction plate, the amplification plate, and the lid respectively, place the extraction plate on the extraction plate transfer member 53, place the amplification plate on the amplification plate transfer member 54, and place the lid at the lid placement position 721 of the placement member 72. The transfer mechanism is provided within the movement range of the gripping part 40. Therefore, the transfer paths of the extraction plate from the transfer mechanism to the extraction plate transfer member, the transfer path of the amplification plate from the transfer mechanism to the amplification plate transfer member, and the transfer path of the lid from the transfer mechanism to the placement member 72 by the gripping part 40 can all be overlapped, making the structure more compact.

[0051] Among them, below the gripping part 40, the running rails of the transfer mechanism, the running rails of the transfer member 50, the running rails of the transfer member 32, and the running rails of the placement member 72 are sequentially arranged along the moving direction of the gripping part 40, making the structure more compact.

[0052] Note that the terms used herein are merely for explaining specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless otherwise expressly stated in the present specification, the singular forms also include the plural forms. Also, when the terms "include" and / or "comprise" are used in the present specification, it should be understood that the presence of features, steps, operations, devices, components and / or combinations thereof is expressly indicated.

[0053] Unless otherwise specified, the relative arrangements, mathematical formulas and numerical values of the members and steps described in these examples do not limit the scope of the present invention. For the sake of convenience of explanation, it goes without saying that the dimensions of each part shown in each figure are not drawn in accordance with the actual proportional relationship. Technologies, methods, and devices known to those skilled in the art may not be examined in detail, but when appropriate, the above-mentioned technologies, methods, and devices should be regarded as part of the specification. In all the examples described and explained herein, any specific value should be construed as merely an example and not a limiting one. Therefore, in examples other than the exemplary embodiments, different values may be used. Note that similar reference numerals and alphabets indicate similar ones in the following drawings. Once any one of them is defined in a drawing, no further consideration is required in subsequent drawings.

[0054] In the description of the present invention, for example, the orientation or positional relationship indicated by orientation terms such as "front, rear, upper, lower, left, right", "lateral direction, longitudinal direction, vertical, horizontal" and "top, bottom" is usually based on the orientation or positional relationship shown in the figure, and is merely for the convenience of explanation and simplification of the description of the present invention. In the absence of a contrary description, these orientation terms do not indicate or imply that the device or element to be indicated must have a specific orientation or be configured and operated in a specific orientation, and thus should not be understood as limiting the protection scope of the present invention. The orientation term "inside, outside" means inside and outside with respect to the contour of each member itself.

[0055] For the sake of convenience in description, spatial relative terms such as, for example, "above...", "above...", "on the upper surface of...", "upper surface of...", etc. can be used to describe the spatial positional relationship between one device or feature shown in the figure and other devices or features. The spatial relative terms are intended to encompass the situation where the device is in a different orientation other than the orientation illustrated during use or operation. For example, if the device is inverted in the figure, a device described as "above other devices or structures" or "on other devices or structures" will then be positioned as "below other devices or structures" or "under other devices or structures". Therefore, the exemplary term "above..." includes two orientations, namely "above..." and "below...". The device may be positioned in other different ways (rotated by 90 degrees or in other orientations), and the description of the space used here should be interpreted accordingly.

[0056] In addition, the use of terms such as "first", "second", etc. to limit components is only for the purpose of facilitating the distinction of the corresponding components. Unless otherwise stated, the above terms have no special meaning and should not be understood as limiting the protection scope of the present invention.

[0057] The above are only preferred embodiments of the present invention and do not limit the present invention. A person skilled in the art can make various modifications and changes to the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should all be included within the protection scope of the present invention.

Description of Reference Numerals

[0058] 10: Frame body, 11: Sample processing platform, 12: First guide rail, 13: Second guide rail, 20: Specimen loading member, 30: Extraction mechanism, 31: Extraction member, 32: Transfer member, 40: Gripping portion, 50: Transfer member, 53: Extraction plate transfer member, 54: Amplification plate transfer member, 60: Liquid transfer portion, 61: Sample arm, 62: Liquid transfer needle, 70: Film sealing mechanism, 71: Heat press member, 72: Placing member, 721: Cover placing position, 722: Amplification plate placing position, 73: Film sealing frame, 80: Consumable storage bin.

Claims

1. a frame (10) having a sample processing platform (11); an extraction mechanism (30) for extracting an analyte in a specimen; a gripping part (40) movably provided above the sample processing platform (11) along a vertical direction and a first horizontal direction; a transfer member (50) movably provided on the sample processing platform (11) along a second horizontal direction perpendicular to the first horizontal direction; a liquid transfer part (60) movably provided above the sample processing platform (11), and comprising: the transfer member (50) includes an extraction plate transfer member (53) having a first transfer position that moves corresponding to below the gripping part (40); the gripping part (40) has a first gripping position located above the extraction plate transfer member (53) and a second gripping position located above the extraction mechanism (30); when the extraction plate transfer member (53) is located at the first transfer position, the gripping part (40) can move between the first gripping position and the second gripping position so as to move an extraction plate between the extraction plate transfer member (53) and the extraction mechanism (30); the extraction plate transfer member (53) further has a sample addition position that moves to the corresponding liquid transfer part (60); when the extraction plate transfer member (53) is located at the sample addition position, the liquid transfer part (60) further has a first liquid transfer position that moves above the extraction plate transfer member (53); the extraction mechanism (30) includes an extraction member (31) and a transfer member (32) movably formed in the extraction member (31) along the second horizontal direction; the transfer member (32) has a transfer position located corresponding to below the gripping part (40) and an extraction position located below the extraction member (31); when the extraction plate transfer member (53) is located at the first transfer position and the transfer member (32) is located at the transfer position, the gripping part (40) can move the extraction plate between the extraction plate transfer member (53) and the transfer member (32); below the gripping part (40), a running rail of the transfer member (50) and a running rail of the transfer member (32) are sequentially arranged along the moving direction of the gripping part (40). A specimen processing apparatus characterized by the following.

2. The transfer member (50) further includes an amplification plate transfer member (54), both the extraction plate transfer member (53) and the amplification plate transfer member (54) move along the second horizontal direction, the amplification plate transfer member (54) has a nucleic acid addition position flush with the extraction plate transfer member (53), the liquid transfer unit (60) further has a second liquid transfer position corresponding to above the amplification plate transfer member (54), when the extraction plate transfer member (53) is located at the sample addition position and the amplification plate transfer member (54) is located at the nucleic acid addition position, the liquid transfer unit (60) can switch between the first liquid transfer position and the second liquid transfer position. The specimen processing apparatus according to claim 1, characterized by the above.

3. further includes a film sealing mechanism (70) for sealing the amplification plate, the film sealing mechanism (70) includes a placement member (72) and a heat press member (71) for sealing the amplification plate, the heat press member (71) is located above the placement member (72), the placement member (72) is provided movably along the second horizontal direction on the sample processing platform (11), the placement member (72) has an amplification plate placement position (722) for placing the amplification plate, the amplification plate transfer member (54) has a second transfer position located below the gripping portion (40), the gripping portion (40) further has a third gripping position located above the placement member (72) and a fourth gripping position located above the amplification plate transfer member (54), when the amplification plate transfer member (54) is located at the second transfer position, the gripping portion (40) can move between the third gripping position and the fourth gripping position so as to move the amplification plate from the amplification plate transfer member (54) to the amplification plate placement position (722). The specimen processing apparatus according to claim 2, characterized by the above.

4. the placement member (72) further has a lid placement position (721) for placing the lid, When the gripping part (40) is located at the third gripping position, the placing member (72) has: a separation position where the lid placing position (721) moves below the gripping part (40) and the gripping part (40) grips the lid; a release position where the amplification plate placing position (722) moves below the gripping part (40) and the gripping part (40) releases the lid onto the amplification plate; and a sealing position where the amplification plate placing position (722) moves below the hot press member (71). The specimen processing apparatus according to claim 3, characterized in that.

5. The film sealing mechanism (70) includes a film sealing frame (73) and a detection member. The film sealing frame (73) is provided on the sample processing platform (11). Both the hot press member (71) and the detection member are fixedly installed on the film sealing frame (73). The placing member (72) is provided movably along the second horizontal direction on the film sealing frame (73). The detection member can detect the placement state of the placing member (72). The specimen processing apparatus according to claim 4, characterized in that.

6. The lid placing position (721) has a first position limiting protrusion for limiting the position of the lid, and / or The amplification plate placing position (722) has a second position limiting protrusion for limiting the position of the amplification plate. The specimen processing apparatus according to claim 4, characterized in that.

7. It further includes a consumable storage bin (80) for placing a liquid transfer head. The liquid transfer part (60) has a load position where it moves above the consumable storage bin (80) to load the liquid transfer head. The specimen processing apparatus according to claim 1, characterized in that.

8. The liquid transfer part (60) includes a sample arm (61) and a liquid transfer needle (62). The sample arm (61) is provided movably horizontally on the frame body (10) and is located above the sample processing platform (11). The liquid transfer needle (62) is provided movably in the vertical direction on the sample arm (61). The specimen processing apparatus according to claim 1, characterized in that.

9. The frame body (10) further includes a first guide rail (12) and a second guide rail (13) located above the sample processing platform (11). The first guide rail (12) extends in the first horizontal direction, and the second guide rail (13) extends in the second horizontal direction. The first guide rail (12) is provided below the second guide rail (13) so as to be movable along the extending direction of the second guide rail (13). The sample arm (61) is provided on the first guide rail (12) so as to be movable along the extending direction of the first guide rail (12). The specimen processing apparatus according to claim 8, wherein

10. The gripping portion (40) includes a gripping frame and a gripper movably provided in the vertical direction on the gripping frame. The gripping frame is provided on the frame body (10) so as to be movable along the first horizontal direction. The gripper has the first gripping position and the second gripping position. The specimen processing apparatus according to claim 1, wherein

11. A reagent preparation apparatus for preparing extraction reagents and amplification reagents, An amplification detection apparatus for performing analyte detection measurement on a measurement mixture to analyze a specimen, A nucleic acid detection integrated machine, comprising the specimen processing apparatus according to any one of claims 1 to 10.

12. The reagent preparation apparatus, the specimen processing apparatus, and the amplification detection apparatus are isolated from each other. The reagent preparation apparatus and the amplification detection apparatus are respectively located on both sides of the specimen processing apparatus. A first passage is provided between the reagent preparation apparatus and the specimen processing apparatus, and a second passage is provided between the specimen processing apparatus and the amplification detection apparatus. Both the first passage and the second passage have a blocked state and a conductive state, and / or Further includes a delivery mechanism provided between the reagent preparation apparatus and the specimen processing apparatus. The delivery mechanism can move an extraction plate for preparing extraction reagents, an amplification plate for preparing amplification reagents, and a lid body through the first passage to the specimen processing apparatus. The gripping portion (40) of the specimen processing apparatus can move the extraction plate to the extraction plate transfer member (53) of the specimen processing apparatus and move the amplification plate to the amplification plate transfer member (54) of the specimen processing apparatus. The nucleic acid detection integrated machine according to claim 11, wherein

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