Materials and Methods for Differential Characterization of Molecular Conjugates and Conjugates

Abstract

A method for analyzing drug-polypeptide conjugates using tandem mass spectrometry involving dual tandem mass tags (TMT) is described. An integrated method using TMT to obtain three analytical measurements, termed a "triple run," is provided herein, which enables the triggering of an additional MS / MS assay to determine drug occupancy, normalize across multiple samples, and identify and localize the payload conjugation site. Also described is a method for multiplexing conjugation reactions in a single run using TMT labeling for enhanced throughput capabilities while maintaining the same sensitivity as current mass spectrometers.

Description

[Technical Field]

[0001] This application claims priority to U.S. Provisional Application No. 62 / 902,958, filed September 19, 2019, the disclosure of which is incorporated herein by reference in its entirety.

[0002] This application relates, inter alia, to molecular conjugates comprising conjugated molecules, e.g., methods and reagents for characterizing molecular conjugates, including quantifying, normalizing, detecting, and / or identifying conjugated molecules. As taught herein, the present invention is useful for use with various types of molecules, including, for example, molecular conjugates, drug conjugates such as antibody-drug conjugates (ADCs), and the like. [Background technology]

[0003] Conjugation of molecules can be advantageous. For example, drug conjugates, such as antibody-drug conjugates (ADCs), are an expanding class of therapeutics for treating cancer (Polakis, Pharmacological Reviews 68, 3-19 (2016)) and immunological disorders (McPherson, Methods in Molecular Biology 2078, 23-36 (2020)). The general structure of an ADC contains a monoclonal antibody (mAb) connected to a drug via a cleavable or non-cleavable linker. For example, an ADC can have a humanized / human mAb connected to a biologically active payload, such as a cytotoxic agent, a steroid, or an antisense oligonucleotide, via a non-cleavable or cleavable linker, such as an acid-labile linker, a protease-cleavable linker, or a disulfide linker. The linker can be covalently attached to the mAb at the conjugation site via lysine coupling, cysteine ​​alkylation, or enzymatic reaction. ADCs bind to their target cell surface antigen receptors, which allows for targeted delivery of drugs to the target site and minimizes systemic toxic effects on healthy tissue, resulting in increased selectivity and improved efficacy and safety over alternative chemotherapeutics or other first-generation mAbs (see, e.g., Dan et al., 2018, Pharmaceuticals (Basel) 11).

[0004] Complete conjugation is difficult to achieve. An incomplete conjugation process can result in free or unconjugated drug, drug linker, or drug-related impurities. Degradation products can arise over time in formulations and in vivo. Characterization and quantification of ADCs are important for their safety, efficacy, and uniformity. Summary of the Invention [Means for solving the problem]

[0005] The present application provides improved, e.g., more accurate, reliable, sensitive, etc., materials and methods for the efficient identification and quantification of molecular conjugates, including therapeutic conjugates such as drug conjugates. The present invention teaches, for example, materials and methods for such improved characterization.

[0006] The present application relates, inter alia, to a method for analyzing conjugates comprising a drug covalently attached to a polypeptide by using Tandem Mass Tags (TMT).

[0007] In one aspect, the present application provides a method for analyzing a conjugate comprising a drug covalently attached to a polypeptide, the method comprising: (i) contacting a sample containing the conjugate with a first tandem mass tag (TMT), thereby labeling the polypeptide of the conjugate with the first TMT; (ii) digesting the polypeptide of the first TMT-labeled conjugate to produce a first mixture comprising one or more unlabeled peptides and one or more peptides labeled with the first TMT; (iii) contacting the first mixture with a second tandem mass tag (TMT), thereby obtaining a second mixture comprising one or more peptides labeled with at least one of the first TMT and the second TMT, and optionally one or more unlabeled peptides, wherein the first TMT and the second TMT do not have the same reporter ion mass; (iv) subjecting the second mixture to liquid chromatography (LC) to produce an LC eluate; (v) subjecting the eluate to tandem mass spectrometry to obtain mass spectra of peptides containing at least one reporter ion of the first TMT and the second TMT; (vi) detecting the mass-to-charge ratio (m / z) associated with the at least one reporter ion, thereby analyzing the conjugate in the sample.

[0008] In another embodiment, a sample containing a conjugate of a drug covalently attached to a polypeptide is analyzed along with a control sample containing a polypeptide that is not covalently attached to a drug. (i) contacting a sample containing the conjugate with a first tandem mass tag (TMT), thereby labeling the polypeptide of the conjugate with the first TMT; (ii) digesting the polypeptide of the first TMT-labeled conjugate to produce a first mixture comprising one or more unlabeled peptides and one or more peptides labeled with the first TMT; (iii) contacting the first mixture with a second tandem mass tag (TMT), thereby obtaining a second mixture comprising one or more peptides labeled with at least one of the first TMT and the second TMT, and optionally one or more unlabeled peptides; (iv) contacting a control sample containing a polypeptide that is not covalently bound to a drug with a third TMT to label the polypeptide that is not covalently bound to a drug with the third TMT; (v) digesting the polypeptides that are not covalently attached to the third TMT-labeled drug to produce a third mixture comprising one or more unlabeled peptides and one or more peptides labeled with the third TMT; (vi) contacting the third mixture with a fourth tandem mass tag (TMT), thereby obtaining a fourth mixture comprising one or more peptides labeled with at least one of the third TMT and the fourth TMT, and optionally one or more unlabeled peptides; (vii) combining the second mixture with the fourth mixture and subjecting the combination to liquid chromatography (LC) to generate an LC eluate; (viii) subjecting the eluate to tandem mass spectrometry to obtain mass spectra of peptides containing reporter ions of at least one of the first TMT, the second TMT, the third TMT, and the fourth TMT; (ix) detecting a mass-to-charge ratio associated with at least one reporter ion, thereby analyzing the conjugate in the sample; None of the first, second, third, and fourth TMTs have the same reporter ion mass, the first and third TMTs are selected from a first isobaric set of TMTs, the second and fourth TMTs are selected from a second isobaric set of TMTs, the first isobaric set of TMTs are reactive to unconjugated amino acid residues capable of forming covalent bonds with a drug, and the second isobaric set of TMTs are reactive to lysines or free amines at the N-terminus of a peptide.

[0009] In one embodiment, the method of the present application is used to determine the occupancy of conjugation sites in a conjugate. For example, the individual site occupancy of a conjugate can be determined by 1) obtaining mass spectra using the method of the present application for a peptide labeled with both a first TMT and a second TMT and a peptide labeled with both a third TMT and a fourth TMT, the mass spectra including a reporter ion of the first TMT, a reporter ion of the third TMT, a reporter ion of the second TMT, and a reporter ion of the fourth TMT; 2) detecting the mass-to-charge ratio (m / z) associated with the reporter ions in the mass spectrum; and 3) determining the occupancy of the conjugation sites in the conjugate based on the intensities of the reporter ions of the first TMT and the third TMT, or the intensities of the reporter ions of the second TMT and the fourth TMT, in the mass spectrum, preferably, the occupancy being calculated based on the following formula: (intensity of reporter ion of third TMT - intensity of reporter ion of first TMT) / intensity of reporter ion of third TMT, or The intensity of the reporter ion of the fourth TMT can be determined by (intensity of the reporter ion of the fourth TMT - intensity of the reporter ion of the second TMT) / intensity of the reporter ion of the fourth TMT.

[0010] In certain embodiments, the occupancy of the conjugation sites in the conjugate is determined at various time points, and additional TMTs are used to label the polypeptide at different time points.

[0011] In another embodiment, the method of the present application is used to normalize a conjugate sample with a control sample. For example, a conjugate sample is normalized with a control sample by a method comprising: 1) obtaining mass spectra for a peptide labeled with only a second TMT and a peptide labeled with only a fourth TMT using the method of claim 2, wherein the mass spectra include a reporter ion of the second TMT and a reporter ion of the fourth TMT, but not a reporter ion of the first TMT or a third TMT; 2) detecting the mass-to-charge ratio (m / z) associated with the reporter ions; and 3) normalizing the sample with the control sample by the ratio of the intensity of the reporter ion of the second TMT to the intensity of the reporter ion of the fourth TMT.

[0012] In yet another embodiment, the methods of the present application are used to localize drug conjugation sites, e.g., sites where a drug is covalently attached to a polypeptide in a conjugate. For example, the drug conjugation site can be localized by a method comprising: 1) obtaining a mass spectrum of a peptide labeled with only a second TMT using the methods of the present invention, the mass spectrum including only the reporter ion of the second TMT, but not the reporter ions of the first, third, or fourth TMTs; and 2) triggering a second tandem mass spectrometry analysis of the peptide labeled with only the reporter ion of the second TMT, thereby localizing the drug conjugation site. In certain embodiments, the peptide labeled with only the reporter ion of the second TMT obtained after digestion of the second mixture is fully conjugated to the drug, e.g., all amino acid residues on the peptide that can be conjugated to the drug are covalently attached to the drug. A peptide can be conjugated to only one drug if it contains only one amino acid residue (e.g., Cys or Lys) capable of forming a covalent bond with a drug, or it can be conjugated to more than one drug if it contains two or more amino acid residues (e.g., Cys or Lys) capable of forming a covalent bond with a drug.

[0013] In certain embodiments, a method for localizing a drug conjugation site in a conjugate includes: 1) obtaining a mass spectrum of a peptide labeled with only the first and second TMTs using a method of the present application, the mass spectrum including only the reporter ions of the first and second TMTs, but not the reporter ions of the third or fourth TMTs; and 2) triggering a second tandem mass spectrometry analysis of the peptide labeled with only the first and second TMTs, thereby localizing the drug conjugation site. In certain embodiments, the peptide labeled with only the first and second TMTs obtained after digestion of the second mixture is not fully conjugated to the drug, e.g., only some, but not all, amino acid residues available for conjugation to the drug are covalently attached to the drug.

[0014] In one embodiment of the present application, the conjugate is a drug-antibody conjugate (ADC), more preferably the ADC comprises a drug covalently attached to one or more cysteine ​​(Cys) or lysine (Lys) residues of a monoclonal antibody.

[0015] As will be appreciated by those skilled in the art, various suitable tandem mass spectrometry methods can be used in the methods of the present application in light of the present disclosure. For example, see Friese et al., MAbs 10, 335-345 (2018), the contents of which are incorporated herein by reference in their entirety, for a review of tandem mass spectrometry methods. In certain embodiments, high-energy collision-induced dissociation tandem mass spectrometry (HCD-MS2) is used to obtain mass spectra of peptides containing reporter ions of at least one of a first TMT, a second TMT, a third TMT, and a fourth TMT. In other embodiments, after one or more TMT reporter ions are detected from a peptide from an HCD-MS2 analysis, an additional dissociation analysis is triggered for further characterization of the peptide, e.g., to localize drug conjugation sites in the peptide. The additional dissociation can be performed by a second tandem mass spectrometry analysis. Examples of second tandem mass spectrometry techniques useful in such methods include, but are not limited to, electron transfer dissociation tandem mass spectrometry (ETD-MS2) or electron-capture dissociation tandem mass spectrometry (ECD-MS2).

[0016] In other embodiments, a higher trigger intensity threshold and / or a narrower isolation window are used to improve triggering of the second tandem mass spectrometry. In certain embodiments, synchronous precursor selection using tribrid technology is applied to tandem mass spectrometry to improve the specificity and accuracy of detection and quantification.

[0017] In view of the present disclosure in this application, any suitable TMT can be used. For example, for a review of TMT, see Bachor et al., Molecules 2019, 24, 701, the contents of which are incorporated herein by reference in their entirety. In some embodiments of the present application, the first isobaric set of TMTs includes two or more TMTs reactive to reduced cysteines. Preferably, the first isobaric set of TMTs includes two, three, four, five, six, or more TMTs reactive to reduced cysteines. In some embodiments, the first isobaric set includes two, three, four, five, six, or more isobaric isomers (e.g., of the same mass and structure) that are iodoacetyl-activated for covalent, irreversible labeling of sulfhydryl (—SH) groups. In some embodiments, the first isobaric set comprises two, three, four, five, or six isobaric isomers of the IodoTMTsixplex isobaric labeling reagent set available from ThermoFisher Scientific (catalog number 90101).

[0018] In certain embodiments, the first and third TMTs each comprise a mass reporter, a mass normalizer, and a cysteine-reactive group covalently linked to each other. Thus, each of the first and third TMTs labels reduced cysteines in the polypeptide of the conjugate and can be used to analyze conjugates containing drugs covalently attached to one or more cysteine ​​residues of the polypeptide. Preferably, each of the first and third TMTs is selected from the isobaric set of IodoTMTsixplex.

[0019] In some embodiments of the present application, the second isobaric set of TMTs includes two, three, four, five, six, or more TMTs reactive with lysine or primary amines at the N-terminus of peptides. In one embodiment of the present application, the second isobaric set of TMTs includes two, three, four, five, six, seven, eight, nine, ten, or more isobaric compounds having an amine-reactive NHS-ester group, a spacer arm, and a mass reporter. For example, the second isobaric set can include two, three, four, five, six, or more isobaric isomers of TMT10plex™ (ThermoFisher, Catalog No. 90110), TMTsixplex™ (ThermoFisher, Catalog No. 90061), or TMTpro™16plex (ThermoFisher, Catalog No. A44520) labeling reagent sets.

[0020] In certain embodiments, the second and fourth TMTs each comprise a mass reporter, a mass normalizer, and an amine-reactive group covalently linked to each other. Thus, each of the second and fourth TMTs labels the lysine or N-terminus of a conjugated polypeptide and can be used with the first and third TMTs for the analysis of conjugates containing a drug covalently attached to one or more cysteine ​​residues of the polypeptide. Preferably, each of the second and fourth TMTs is selected from the isobaric sets TMT6plex, TMT10plex, or TMT pro16plex.

[0021] In some embodiments, the first isobaric set and the second isobaric set of TMTs each independently comprise two, three, four, five, six, or more TMTs reactive with lysines or primary amines at the N-terminus of a peptide. In certain embodiments, the first, second, third, and fourth TMTs each comprise a mass reporter, a mass normalizer, and an amine-reactive group covalently linked to each other. The TMTs can be used to analyze conjugates comprising a drug covalently attached to one or more lysine residues of a polypeptide.

[0022] In some embodiments, multiplexes of samples containing one or more conjugates are analyzed together. In certain embodiments, the drug conjugation sites in the conjugates are characterized by mass spectrometry barcodes containing (2n+2) reporter ions, and the conjugates are normalized by mass spectrometry barcodes containing n+1 reporter ions, where n is the number of samples analyzed by this method.

[0023] Another aspect of the present application relates to a system for carrying out any of the methods described in claims 1-23.

[0024] Another aspect of the present application relates to a composition comprising a mixture of peptides labeled with at least one of a first TMT and a second TMT and, optionally, one or more unlabeled peptides, wherein the first TMT and the second TMT do not have the same reporter ion mass, and the mixture of peptides comprises at least one peptide conjugated to a drug and labeled with at least one of the first TMT and the second TMT.

[0025] Other aspects, features, and advantages of the present invention will become apparent from the following disclosure, which is illustrative and non-limiting, including the detailed description and preferred embodiments thereof, and the appended claims, as will be appreciated by those skilled in the art. [Brief explanation of the drawings]

[0026] The foregoing summary, as well as the following detailed description of preferred embodiments of the present application, will be better understood when read in conjunction with the appended drawings. It should be understood, however, that the present application is not limited to the precise embodiments shown in the drawings.

[0027] This patent or application file contains at least one drawing executed in color. Copies of this patent or patent application publication with color drawing(s) will be provided by the Office upon request and payment of the necessary fee.

[0028] Unless otherwise indicated, in all figures herein, the reporter ions for IdoTMT are

[0029] [ka] and the reporter ion for TMT6 is

[0030] [ka] The analyzed peptides are

[0031] [ka] and drugs are

[0032] [ka] It is expressed as:

[0033] [Figure 1A] The following are exemplary tandem mass tag (TMT) structures useful for labeling cysteines in polypeptides: Figure 1A - IodoTMTsixplex set; Figure 1B - TMTsixplex set; Figure 1C - TMT10plex set; Figure 1D - TMTpro16plex (including TMT chemical structures and 13C and 15N stable isotope positions (*)). [Figure 1B] The following are exemplary tandem mass tag (TMT) structures useful for labeling cysteines in polypeptides: Figure 1A - IodoTMTsixplex set; Figure 1B - TMTsixplex set; Figure 1C - TMT10plex set; Figure 1D - TMTpro16plex (including TMT chemical structures and 13C and 15N stable isotope positions (*)). [Figure 1C]The following are exemplary tandem mass tag (TMT) structures useful for labeling cysteines in polypeptides: Figure 1A - IodoTMTsixplex set; Figure 1B - TMTsixplex set; Figure 1C - TMT10plex set; Figure 1D - TMTpro16plex (including TMT chemical structures and 13C and 15N stable isotope positions (*)). [Figure 1D] The following are exemplary tandem mass tag (TMT) structures useful for labeling cysteines in polypeptides: Figure 1A - IodoTMTsixplex set; Figure 1B - TMTsixplex set; Figure 1C - TMT10plex set; Figure 1D - TMTpro16plex (including TMT chemical structures and 13C and 15N stable isotope positions (*)). [Figure 2] The creation of unique barcodes according to embodiments herein for sample characterization is demonstrated, for example, to quantify (e.g., drug occupancy), normalize (e.g., normalize mock to conjugate), and detect (e.g., localize drug conjugation sites) samples based on the intensity and combination of predetermined TMT reporters (patterns) detected from tandem mass spectrometry (MS2) analysis. [Figure 3A] An embodiment of the present application is shown in which MS barcodes are created via triple play by sequential dual TMT labeling of conjugates with a drug (Drug, D), e.g., its antibody or fragment conjugated with the drug, and a mimic, e.g., its antibody or fragment not conjugated with the drug, using an analytical scheme that can be quantified, normalized, and localized. [Figure 3B] 1 illustrates a process using dual TMT to quantify drug conjugates with one drug (D) coupled to a peptide obtained by trypsin digestion, according to an embodiment of the present application. [Figure 3C] 1 illustrates a process using dual TMT to quantify drug conjugates with up to two drugs (D1 and D2) conjugated to peptides obtained by trypsin digestion, according to one embodiment of the present application. [Figure 3D]1 illustrates the use of dual TMT to quantify drug conjugates with up to three drugs (D1, D2, and D3) coupled to peptides obtained by trypsin digestion, according to an embodiment of the present application. [Figure 4] Several exemplary experimental ADC systems analyzed by the methods of the present invention are shown, including biotin-PEO acetamide conjugates formed by conjugation of iodoacetamide (IAA) and biotin-PEO iodoacetamide, HSA peptides containing one to three cysteines conjugated to NIST mAb or N-(7-dimethylamino-4-methyl-3-coumarinyl)maleimide (DACM-3), and the ADC standard MSQC8 (densyl fluorophore LC-SMCC crosslinker) from Sigma. [Figure 5A] Although each shows an embodiment of the present application using dual TMT to quantify site-specific ADC on NIST mAb by performing HCD-MS2, other MS2 such as ETD-MS2 can also be applied to quantify drug occupancy for specific residues using, for example, iodoTMT (io, labels Cys residues or C) or TMT (tm, labels Lys residues or N-terminal amine) reporter ion intensities and calculate the formula for % occupancy, where the ADC is NIST mAb-biotin-PEOacetamide. [Figure 5B] Although each shows an embodiment of the present application using dual TMT to quantify site-specific ADC on NIST mAb by performing HCD-MS2, other MS2 such as ETD-MS2 can also be applied to quantify drug occupancy for specific residues using, for example, iodoTMT (io, labels Cys residues or C) or TMT (tm, labels Lys residues or N-terminal amine) reporter ion intensities and calculate the formula for % occupancy, where the ADC is NIST mAb-biotin-PEOacetamide. [Figure 5C] The corresponding MS2 spectrum (with all backbone product ions) of the peptide sequenced by HCD-MS2 is shown. [Figure 5D]The corresponding MS2 spectrum (with all backbone product ions) of the peptide sequenced by HCD-MS2 is shown. [Figure 6A] Although the embodiment of this application shows the use of TMT to normalize the mixing ratio of NIST mAb by performing HCD-MS2 across reaction conditions with non-cysteine ​​peptides, other MS2, such as other ETD-MS2, can also be used, and the normalization ratio shown in the formula can be used to correct for mixing or digestion bias between the ADC (NIST mAb-biotin-PEO acetamide) containing sample (sample) and the unconjugated sample (mock). [Figure 6B] Although the embodiment of this application shows the use of TMT to normalize the mixing ratio of NIST mAb by performing HCD-MS2 across reaction conditions with non-cysteine ​​peptides, other MS2, such as other ETD-MS2, can also be used, and the normalization ratio shown in the formula can be used to correct for mixing or digestion bias between the ADC (NIST mAb-biotin-PEO acetamide) containing sample (sample) and the unconjugated sample (mock). [Figure 7] Using backbone fragmentation of a peptide consisting of a drug and analysis using methods according to embodiments of the present application and ETD-MS2, the HCD-MS2 product ion spectrum, which also includes fragments corresponding to fragmentation of the biotin-PEO-acetamide molecule, is shown. Note that small molecule conjugates are prone to complex spectra generated by fragmentation, and the fragment masses derived from the drug are specific to each drug. [Figure 8A] Figure 1 shows the TMT-130 reporter trigger for the NIST triple play workflow. [Figure 8B] Using methods according to embodiments of the present application and observed total ion chromatograms (TICs), we demonstrate the use of TMT-130 signature ions (barcodes) to trigger ETD-MS2 and localize conjugated drugs. [Figure 9A]Using methods according to embodiments of the present application, we show the localization of biotin-PEO-acetamide via the mass trigger of TMT-130: TMT-130 from HCD-MS2 is a unique trigger and is independent of the type of ADC and drug localized by ETD-MS2 without drug fragmentation: D = biotin-PEO-acetamide. [Figure 9B] Using methods according to embodiments of the present application, we show the localization of biotin-PEO-acetamide via the mass trigger of TMT-130: TMT-130 from HCD-MS2 is a unique trigger and is independent of the type of ADC and drug localized by ETD-MS2 without drug fragmentation: D = biotin-PEO-acetamide. [Figure 9C] The trigger mass detection corresponds to biotin-PEO-acetamide conjugated at Cys-23, and TMT130 is less abundant than the immonium ion generated by biotin-PEO-acetamide. [Figure 9D] The trigger mass detection corresponds to biotin-PEO-acetamide conjugated at Cys-23, and TMT130 is less abundant than the immonium ion generated by biotin-PEO-acetamide. [Figure 10A] The following methods of improving the specificity of the trigger TMT-130 according to embodiments of the present application are shown: co-isolation and co-fragmentation of peptides resulted in a loss of specificity of TMT-130, whereas a higher trigger intensity threshold or narrower isolation window resulted in improved mass triggering. [Figure 10B] The following methods of improving the specificity of the trigger TMT-130 according to embodiments of the present application are shown: co-isolation and co-fragmentation of peptides resulted in a loss of specificity of TMT-130, whereas a higher trigger intensity threshold or narrower isolation window resulted in improved mass triggering. [Figure 11]FIG. 1 shows how the Sample Prep Station (SPS-3) is used in an isobaric labeling experiment according to an embodiment of the present application, where precursor ions are transferred from the Ion Routing Multipole (IRM) to the Ion trap (IT), and synchronous precursor selection (SPS) is performed in the IT. [Figure 12A] Improved specificity and accuracy of TMT quantification using synchronous precursor selection according to embodiments of the present application are shown in Figure 12A, which shows the MS2 TMT reporter for synchronous precursor ion selection (top 5 fragments). [Figure 12B] Figure 12B shows improved specificity and accuracy of TMT quantification using synchronous precursor selection according to embodiments of the present application. Figure 12B shows that SPS-MS3 improves quantification sensitivity and accuracy upon simultaneous solubility of interferences. [Figure 12C] Figure 12C shows improved specificity and accuracy of TMT quantification using synchronous precursor selection according to embodiments of the present application. Figure 12C shows reporter quantification, where the ADC is a SigmaMAb ADC mimic (MSQC8)-human universal mAb standard conjugated to a dansyl fluorophore, and the sham is a human universal mAb standard (MSQC4, an IgG1 mAb) Sigma mAb not conjugated to a drug. [Figure 13A] Experiments and results using dual TMT to quantify site-specific ADC (dansyl fluorophore) on MSQC8 (mAb antibody-drug conjugate mimic) using methods according to embodiments of the present application, including MS-based barcoding for site-specific conjugate occupancy, are presented. [Figure 13B] Experiments and results using dual TMT to quantify site-specific ADC (dansyl fluorophore) on MSQC8 (mAb antibody-drug conjugate mimic) using methods according to embodiments of the present application, including MS-based barcoding for site-specific conjugate occupancy, are presented. [Figure 13C]Experiments and results using dual TMT to quantify site-specific ADC (dansyl fluorophore) on MSQC8 (mAb antibody-drug conjugate mimic) using methods according to embodiments of the present application, including MS-based barcoding for site-specific conjugate occupancy, are presented. [Figure 13D] Experiments and results using dual TMT to quantify site-specific ADC (dansyl fluorophore) on MSQC8 (mAb antibody-drug conjugate mimic) using methods according to embodiments of the present application, including MS-based barcoding for site-specific conjugate occupancy, are presented. [Figure 13E] Experiments and results using dual TMT to quantify site-specific ADC (dansyl fluorophore) on MSQC8 (mAb antibody-drug conjugate mimic) using methods according to embodiments of the present application, including MS-based barcoding for site-specific conjugate occupancy, are presented. [Figure 13F] Experiments and results using dual TMT to quantify site-specific ADC (dansyl fluorophore) on MSQC8 (mAb antibody-drug conjugate mimic) using methods according to embodiments of the present application, including MS-based barcoding for site-specific conjugate occupancy, are presented. [Figure 14A] ADC quantification across the MSQC8 site using a method according to one embodiment of the present application shows that the following known methods correlated with a triple play amount of MSQC8 light chain conjugation: Cys-218 = 60%, which correlated with a DAR0 / DAR1 ratio of 1:2 for light chain SLIM-IMS, and estimated occupancy at Cys-266 (max) = 60% and Cys-372 = 15%, while all other cysteines showed 0% conjugation. [Figure 14B] ADC quantification across the MSQC8 site using a method according to one embodiment of the present application shows that the following known methods correlated with a triple play amount of MSQC8 light chain conjugation: Cys-218 = 60%, which correlated with a DAR0 / DAR1 ratio of 1:2 for light chain SLIM-IMS, and estimated occupancy at Cys-266 (max) = 60% and Cys-372 = 15%, while all other cysteines showed 0% conjugation. [Figure 14C] ADC quantification across the MSQC8 site using a method according to one embodiment of the present application shows that the following known methods correlated with a triple play amount of MSQC8 light chain conjugation: Cys-218 = 60%, which correlated with a DAR0 / DAR1 ratio of 1:2 for light chain SLIM-IMS, and estimated occupancy at Cys-266 (max) = 60% and Cys-372 = 15%, while all other cysteines showed 0% conjugation. [Figure 15] We have demonstrated the identification of dansyl fluorophores in MSQC8 using methods according to embodiments of the present application, which showed that fragmentation-prone small molecule conjugates and drug-derived fragment masses that generate complex spectra are specific to each drug. [Figure 16A] 1 illustrates the use of TMT to profile reaction time courses using methods according to embodiments of the present application. [Figure 16B] 1 illustrates the use of TMT to profile reaction time courses using methods according to embodiments of the present application. [Figure 16C] 1 illustrates a triple play workflow applied to monitor responses to multiple drugs (D1, D2, D3, D4, and D5) in a multiplexed manner according to an embodiment of the present application. [Figure 16D] Mass triggering via a single TMT reporter ion specific for each drug molecule is shown. [Figure 17A] We show that the results from TMT analysis correlate with those from fluorescence quantification at high occupancy. [Figure 17B] We show that the results from TMT analysis correlate with those from fluorescence quantification at high occupancy. [Figure 18A] A multiplexing scheme is described for how TMT can be used in various combinations for triple play analysis of up to four ADC samples using dual TMT according to one embodiment of the present application. [Figure 18B] 1 shows the multiplex analysis (using TMT from TMT10plex reagents) of four ADC samples with four different site-specific ADC occupancies according to one embodiment of the present application. [Figure 19A] For example, we show results using the methods of the present invention for high-throughput analysis, analyzing four ADCs in triplicate, achieving dual TMT quantification of four ADC samples in a single run. [Figure 19B] For example, we show results using the methods of the present invention for high-throughput analysis, analyzing four ADCs in triplicate, achieving dual TMT quantification of four ADC samples in a single run. [Figure 19C] For example, we show results using the methods of the present invention for high-throughput analysis, analyzing four ADCs in triplicate, achieving dual TMT quantification of four ADC samples in a single run. [Figure 20A] Here, we demonstrate the use of a non-cysteine ​​peptide sequence, bearing barcodes for five TMT10 reporter ions (one simulant (B) and four conjugate samples (1)–(4)) during MS2-HCD to correct for sample concentrations in multiplexed experiments. The normalization factor for the ith sample is given by Equation 2, and the corrected occupancy for each ADC sample can be obtained by Equation 3: [Figure 20B] Normalized occupancies obtained for four samples in a single acquisition are shown. DETAILED DESCRIPTION OF THE INVENTION

[0034] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Unless otherwise defined, specific terms used herein have the meanings set forth herein. All patents, published patent applications, and publications cited herein are incorporated by reference as if set forth in their entirety.

[0035] Any discussion of documents, operations, materials, devices, articles and the like which has been included in the present specification is for the purpose of providing a context for the present invention. Such discussion is not an admission that any or all of these items constitute part of prior art to any invention disclosed or claimed.

[0036] It should be noted that as used in this specification and the appended claims, the singular forms "a," "an," and "the" include plural referents unless the context clearly dictates otherwise.

[0037] Unless otherwise specified, any numerical values, such as concentrations or concentration ranges, described herein should be understood in all cases to be modified by the term "about." Thus, numerical values ​​typically include ±10% of the stated value. For example, an amount of about 50 ppm or less includes 45 ppm or less to 55 ppm or less. As used herein, the use of numerical ranges explicitly includes all possible subranges, including integers and fractions of values ​​within that range, and all individual numerical values ​​within that range, unless the context clearly indicates otherwise.

[0038] Throughout this specification and the claims that follow, unless the context otherwise requires, the term "comprise" and variations such as "comprises" and "comprising" will be understood to mean the inclusion of a specified integer or step or group of integers or steps, but not the exclusion of any other integer or step or group of integers or steps. As used herein, the term "comprise" may be replaced with the term "containing" or "including," or in some cases, when used herein, may be replaced with the term "having."

[0039] As used herein, "consisting of" excludes any element, step, or ingredient not specified in the claim element. As used herein, "consisting essentially of" does not exclude materials or steps that do not materially affect the basic and novel characteristics of the claim. When used herein in connection with aspects or embodiments of this application, any of the above terms "comprising," "containing," "including," and "having" may be substituted with the terms "consisting of" or "essentially consisting of" in order to vary the scope of the disclosure.

[0040] As used herein, the connective term "and / or" between multiple listed elements is understood to encompass both individual and combined alternatives. For example, when two elements are connected by "and / or," the first alternative refers to the first element being applicable without the second element. The second alternative refers to the second element being applicable without the first element. The third alternative refers to the first and second elements being applicable together. Any one of these alternatives is understood to be included within the meaning and, therefore, meets the requirements of the term "and / or" as used herein. The simultaneous applicability of two or more of the alternatives is also understood to be included within the meaning and, therefore, meets the requirements of the term "and / or."

[0041] As used herein, "MS / MS" or "MS 2" or "MS2" refers to tandem mass spectrometry. Tandem mass spectrometry is a technique in instrumental analysis in which two or more mass analyzers are coupled together using an additional reaction step to increase the ability to analyze a sample. Tandem use of mass spectrometry can be performed where the reaction steps are separated in space (tandem in space) and / or where the reaction steps are separated in time (tandem in time). A common use of tandem mass spectrometry is the analysis of biomolecules such as proteins, peptides, organic and inorganic molecules, lipids, metabolites, and oligonucleotides.

[0042] As used herein, a "reporter ion" or "diagnostic ion" refers to a characteristic product ion of a labeled peptide containing an N-terminal tag or label observed in an ETD mass spectrum. Typically, it is the most predominant product ion in the mass spectrum, which is used to trigger a subsequent MS / MS event to further sequence the labeled peptide.

[0043] As used herein, "tribrid technology" refers to a technology that uses a hybrid mass spectrometer with multiple types of mass analyzers. This allows for the performance of tandem mass spectrometry experiments with great flexibility for TMT sample multiplexing. Tribrid technology can be used to characterize challenging samples, including low-abundance peptides in complex matrices, determining the location and post-translational isoforms of intact proteins, resolving isobaric metabolites, and characterizing protein structure using chemical cross-linking.

[0044] As used herein, "orbitrap" or "OT" refers to an ion trap mass analyzer consisting of two outer electrodes and a central electrode. This configuration allows the orbitrap to function as both an analyzer and a detector. Ions entering the orbitrap are trapped and oscillate between the two outer electrodes, centered on the central electrode. Different ions oscillate at different frequencies, resulting in their separation. The oscillation frequencies induced by the ions on the outer electrodes are measured, and a mass spectrum of the ions is obtained using imaging current detection.

[0045] As used herein, "isobaric" refers to having the same nominal molecular or formula weight. Preferably, isobaric TMTs useful in the present invention have the same mass and structure, and are also referred to as isotopic isomers.

[0046] As used herein, "ion" refers to a molecule that has a net charge due to the loss or gain of one or more electrons. Examples of ions can be product ions of type a, b, or y that result from cleavage of amide bonds along the backbone of a protein by collision-induced dissociation (CID).

[0047] As used herein, a "sequence ion" refers to an ion (or ions) that corresponds to the product of a particular peptide being cleaved at a given peptide bond.

[0048] As used herein, "immonium ion" refers to an ion (or ions) corresponding to an internal fragment of a peptide having a single side chain formed by a combination of -type or y-type cleavages.

[0049] As used herein, a "reporter ion" is an ion that can be detected by methods known in the art (e.g., MS 2 ) refers to the ion cleaved from the isobaric tagged peptide.

[0050] As used herein, "isotope" refers to a molecule that differs from its parent molecule in that at least one atom has a different number of neutrons.

[0051] As used herein, "multipole" refers to an ion guide constructed from metal rods to transport ions through a vacuum system.

[0052] As used herein, "conjugate" refers to a protein or peptide covalently bound to one or more heterologous molecules. Examples of proteins or peptides that can be covalently bound to heterologous molecules include, but are not limited to, therapeutic peptides or proteins, antibodies or fragments thereof. Examples of heterologous molecules that can be covalently bound to a protein or peptide include, but are not limited to, one or more small molecule compounds, labels, etc.

[0053] As used herein, "peptide" refers to an amino acid-based polymer that typically consists of some combination of the 20 common naturally occurring amino acids, but may also contain or be composed entirely of unnatural amino acid monomer residues. It may comprise a linear amino acid polymer structure, or may comprise a cyclic peptide, or any combination of one or all three branched structures. Peptides may also have any combination of naturally occurring modifications (e.g., phosphorylation or glycosylation) or non-naturally occurring modifications (e.g., carbamidomethylation).

[0054] "Antibody" is intended to include (a) immunoglobulin molecules comprising two heavy chains and two light chains and recognizing an antigen, (b) polyclonal or monoclonal immunoglobulin molecules, and (c) monovalent or divalent fragments thereof. Immunoglobulin molecules can be derived from any of the commonly known classes, including, but not limited to, IgA, secretory IgA, IgG, IgE, and IgM. IgG subclasses are well known to those skilled in the art and include, but are not limited to, human IgG1, IgG2, IgG3, and IgG4. Antibodies can be both naturally occurring and non-naturally occurring. Furthermore, antibodies include multispecific antibodies, such as diabodies, triabodies, tetrabodies, fully synthetic antibodies, single-chain antibodies, and fragments thereof. Antibodies can be human or non-human. Antibody fragments include, but are not limited to, Fab fragments, Fv fragments, and other antigen-binding fragments.

[0055] As used herein, "tandem mass tag" or "TMT" refers to a chemical label that can be used for mass spectrometry (MS)-based quantification and identification of molecules. Any molecule with a free thiol or primary amine or glycan can be tagged. For example, the molecule can be a protein or peptide. TMT tags typically contain at least three regions, such as a mass reporter region, a mass normalization or balance region, and a reactive group. The reporter mass can be incremented to create unique masses, while the balance mass can be used to offset the total mass of the reagents, creating a series of reagents with the same overall mass that still have distinct reporter masses. The reactive group facilitates the addition reaction of amines, thiols, oxonium, or other functional groups. It can be, for example, free thiol or reduced cysteine ​​reactive, primary amine or lysine reactive, or aminoxy reactive. Optionally, TMTs can also contain one or more cleavable linker regions.

[0056] An isobaric set of TMTs allows for the simultaneous identification and multiplexed quantification of proteins in different samples using tandem mass spectrometry. An isobaric set of n TMTs can contain one TMT and multiple TMTs with n-1 isotopic substitutions. The chemical structures of all tags in an isobaric set of TMTs are identical, but each While the mass reporter and mass normalization regions contain isotopes substituted at various positions so that each tag has a different molecular weight, the TMTs have the same overall molecular weight (isotropic) and structure so that molecules labeled with different tags are indistinguishable during chromatographic or electrophoretic separation and in a single MS mode. For example, each isobaric reagent can contain a different number of heavy isotopes in the mass reporter region, resulting in a unique reporter mass during tandem MS / MS for sample identification and relative quantification. During fragmentation in MS / MS mode, sequence information is obtained from the fragmentation of the peptide backbone, and quantification data is simultaneously obtained from the fragmentation of the tag, resulting in mass reporter ions. Each TMT in an isobaric set produces a unique reporter mass in the MS / MS spectrum.

[0057] TMT labels, such as commercially available TMT labels, can be multiplexed (e.g., 6-plex, 10-plex, or more) and are reactive to cysteines or amines, respectively. By shifting the position of the heavy isotope between the reporter group and the spacer, the overall mass and chemical structure of each tag can be kept the same (isobaric). For example, the cysteine-reactive IodoTMTsixplex reagent and the amine-reactive TMTsixplex reagent each have six identical reporter ion masses. However, each reporter ion has a unique mass-to-charge ratio (m / z), or reporter mass, also referred to as the reporter ion mass. For example, the nominal reporter masses of the six TMTs in the isobaric TMT6 set range from 126 to 131 Da (Daltons).

[0058] "CysTMT", "IodoTMT", "IodoTMT6" or "IodoTMT 6The "IodoTMTsixplex" isobaric labeling reagent set, also referred to as "IodoTMTsixplex," refers to an isobaric set of six TMTs that are iodoacetyl-activated for covalent, irreversible labeling of sulfhydryl (-SH) groups and have the chemical structures shown in Figure 1A. The TMTs are 13C and / or 15N isotopes connected to reactive groups by spacer arms (Figures 1A-1D). * The IodoTMT reagents contain a signature reporter group (denoted as ). The IodoTMT sixplex isobaric labeling reagent set is commercially available from ThermoFisher Scientific (catalog number 90101). The IodoTMT reagents react specifically with reduced cysteines (Cys) in peptides and proteins. The IodoTMT reagents can be differentiated by mass spectrometry (MS) to allow quantification of the relative abundance of cysteine ​​modifications, such as S-nitrosylation, oxidation, and disulfide bonds, in cultured cells grown or treated under different conditions.

[0059] As used herein, "TMT6", "TMT 6 The "TMTsixplex" isobaric labeling reagent set, also referred to as "TMT6plex," refers to an isobaric set of six TMTs that are NHS-activated for covalent, irreversible labeling of primary amine (-NH) groups and has the chemical structure shown in Figure 1B. The TMTsixplex isobaric labeling reagent set is commercially available from ThermoFisher Scientific (catalog number 90061). This reagent labels all peptides prepared from cell or tissue samples for analysis of up to six samples in a single MS run. According to ThermoFisher Scientific, the TMT6 reagents are optimized for use on high-resolution Thermo Scientific MS / MS platforms, such as the Q Exactive, Orbitrap Elite, Orbitrap Fusion, Tribrid, and Orbitrap Lumos instruments, with data analysis fully supported by Proteome Discoverer 1.0 and above.

[0060] As used herein, "TMT10" and "TMT 10 The "TMT10plex" isobaric labeling reagent set, also referred to as "TMT10 plex," refers to an isobaric set of 10 TMTs, each with an amine-reactive NHS-ester group, a spacer arm, and a mass reporter, and has the chemical structure shown in Figure 1C. The amine-reactive TMT10plex can multiplex up to 10 distinct samples (10-plex). The TMT10plex isobaric labeling reagent set is commercially available from ThermoFisher Scientific (catalog number 90110). It is important to note that the 10-plex reagent still has six nominal masses (126-131 Da), but four of the six reporter masses (127-130 Da) each have two unique reporter ion masses that differ by 6.32 mDa (millidaltons), resulting in a C 12 , N 15 Atom pair is C 13 , N 14 High-resolution mass spectrometry allows accurate relative quantification of these reporter ion masses and their isotopes with baseline resolution. This reagent set allows up to 10 different peptide samples prepared from cells or tissues to be labeled in parallel and then combined for analysis. For each sample, a unique reporter mass in the low-mass region of the high-resolution MS / MS spectrum (i.e., TMT10 126-131 Da) can be used to measure peptide fragmentation and relative protein expression levels during tandem mass spectrometry. According to ThermoFisher Scientific, the TMT10 reagent is optimized for use on high-resolution Thermo Scientific MS / MS platforms such as the Q Exactive, Orbitrap Elite™, and Orbitrap Fusion™ Orbitrap Elite™ Tribrid™ instruments with data analysis fully supported by Proteome Discoverer™ 1.4.

[0061] As used herein, "TMT16", "TMT 16The "TMTpro 16plex" isobaric labeling reagent set, also referred to as "TMTpro" or "TMTpro16 plex," refers to an isobaric set of 16 amine-reactive NHS-ester-activated reagents with the chemical structure shown in Figure 1D. Each of the 16 TMTs has a group at the N-terminus that reacts with lysines or primary amines. TMTpro 16 plex is commercially available from ThermoFisher Scientific (catalog number A44520). According to ThermoFisher Scientific, the TMTpro labeling reagents are next-generation tandem mass tags optimized for use on high-resolution Thermo Scientific MS / MS platforms such as the Q Exactive and Orbitrap Fusion Tririd instrument series, including the Orbitrap Eclipse Tribrid and Orbitrap Exploris 480 mass spectrometers, with data analysis fully supported by Proteome Discoverer 2.3.

[0062] Sets of six reporters (TMT-6 plex), ten reporters (TMT-10 plex), eleven reporters (TMT-11 plex), and up to sixteen reporters (TMT-16 plex) of such TMTs can be used in the methods of the present invention. Given the present disclosure, other examples of TMTs can also be used in the present invention. In particular, TMT-16-plex and future n-plex reagents, where n>16, will increase the number of samples that can be labeled with dual TMT labeling. TMTs can be obtained using methods known in the art or from commercial sources such as ThermoFisher Scientific. However, when two TMTs are used in tandem, they cannot have the same reporter ion mass. For example, IodoTMT and TMT6 have the same set of reporter masses, so if an isobaric set of IodoTMT and TMT6 is used for dual labeling, the selected IodoTMT and TMT must not have the same reporter mass when used for dual labeling. Similarly, when three or more TMTs are used in the same analysis, such as TMTs used together in the analysis of a sample and a mock, or together in the analysis of multiple samples, each of the TMTs used in the analysis must have a unique reporter mass, and none of the TMTs used in the same assay can have the same reporter mass.

[0063] An important metric for ADC efficacy, safety, and selectivity is determined by the drug-antibody ratio (DAR). Chromatographic approaches, such as size exclusion chromatography (SEC), hydrophobic interaction chromatography (HIC), and reversed-phase liquid chromatography (RPLC), can be used to obtain DAR values ​​and characterize ADCs. However, these approaches have significant drawbacks, such as low throughput due to long separation and column regeneration times. Furthermore, the required specific mobile phase prevents on-line coupling to mass spectrometry (MS). Native MS-based approaches preserve noncovalent interactions and, as a result, can obtain information about an array of possible drug-conjugate species.

[0064] Another approach is ion mobility spectroscopy coupled with mass spectrometry (IMS-MS). IMS-MS provides both structural and mass information in a single assay. Consistency in DAR characterization between IMS-MS and MS has been demonstrated. Therefore, IMS-MS can be used to study the drug loading distribution of ADCs and characterize mAbs.

[0065] However, conventional methods for quantifying and identifying drug conjugates suffer from the drawbacks of low sensitivity, poor selectivity, and / or relatively long analytical times or high costs. For example, native MS, chromatography, and IMS-MS approaches provide little information about site-specific drug conjugation of mAbs and whether the occupancy and location of the drug bound to the antibody alters the selectivity or efficacy of the ADC. Identifying peptides with complete drug molecules is difficult due to the small size of the molecule and the linker.

[0066] By normalizing the conjugated peptide intensity by the combined intensity of the unmodified peptide and the conjugated peptide, a ratio can be obtained to quantify the conjugated peptide. This ad hoc ratio facilitates relative estimation of conjugation levels across samples relative to the site of interest. Stoichiometry-based approaches can also be used to indirectly determine modification occupancy by chemically removing the modification or by using stable isotope-labeled synthetic peptides and stable isotope-labeled cell lines (see, e.g., Wu et al., Nature Methods 8, 677-683 (2011); Lim et al., Journal of Proteome Research 16, 4217-4226 (2017)). Primary amine-specific isotropic labeling has been utilized to study the occupancy of a model NIST mAb to demonstrate the utility of a stoichiometry-based approach based on isobaric labeling (Hill et al., Sci Rep 8, 17680 (2018)). Nevertheless, stoichiometry-based quantification methods have not been integrated into pharmaceutical pipelines for quantification or ADC analysis due to, for example, complex and difficult sample preparation, elaborate analytical schemes required to obtain compositional differences based on drug conjugation, etc. (Janin-Bussat et al., Journal of chromatography. B, Analytical technologies in the biomedical and life sciences 981-982, 9-13 (2015); Le et al., Anal Chem 84, 7479-7486 (2012)).

[0067] An isobaric set of TMTs can be used to label and study a set of drug-polypeptide conjugates, such as ADCs. The baseline resolution of these reporter ion masses and their isotopic substitutions allows for accurate relative quantification using high-resolution mass spectrometry. In one aspect of the present application, TMTs are used in tandem mass spectrometry (MS) of samples, particularly drug-drug conjugates, for characterization of the sample's mass spectrometric barcode. 2 ) is used in the analysis of

[0068] A mass spectrum is a histogram obtained using a mass spectrometer. The mass spectrum of an analysis is usually plotted as an intensity (Y) versus m / z (mass-to-charge) ratio (X) plot. The reporter ion for TMT can be detected by its m / z ratio in the mass spectrum. The Y axis represents the signal intensity of the ion.

[0069] Depending on the instrument and software, signal intensity can be measured and expressed differently. For example, when using a counting detector, intensity is often measured in counts per second (cps). When using analog detection electronics, intensity is typically measured in volts. In Fourier transform ion cyclotron resonance mass spectrometry and Orbitrap, the frequency domain signal (y-axis) is related to the power (approximately the squared amplitude) of the signal sine wave (often reduced to the power rms). Some software calculates reporter area rather than intensity. As used herein, with respect to mass spectra, the term "intensity" encompasses the signal intensity of an ion measured by any method and expressed in any form. For example, the "intensity" of a reporting ion can include the intensity of the reporter ion, the reporter area, or any other variation of the signal intensity of an ion from a mass spectrometry analysis.

[0070] As used herein, a "mass spectrometry barcode," "MS barcode," "reporter ion mass barcode," "barcode," or "mass barcode" of a sample refers to a tandem mass spectrometry (MS) barcode that contains information for a set of reporter ions, the measurements of which are unique to the sample. 2 ) analysis. The unique barcode for the sample is used to measure the 2 The analysis may be based on the intensity and / or the presence (pattern) or absence of a set of TMT reporter ions detected from one or more barcodes. One or more barcodes may be used in a triplex prep analysis of a sample.

[0071] Figure 2 shows IodoTMTsixplex and TMT6 for multiplexing six different cell lysates, where labeling can be performed on proteins before digestion or on cysteine-bearing surrogate peptides after digestion of each sample. Each of the six samples is labeled with TMT in the IodoTMT set and analyzed by LC-MS. 2 Combined analysis yields distinct barcodes for each of the six samples. The barcodes are defined by the m / z intensity and pattern of reporter ions generated by TMT on the tandem mass spectrum. Specifically, labeling generates the same peptide isobaric masses for each cell line, indistinguishable by mass. However, after dissociation by either collisional dissociation (HCD) or electron-based dissociation (ETD), unique reporter ions are generated. It is important to note that HCD dissociates the entire reporter, while ETD generates a series of charged fragments that generate a second series of unique reporter ion masses. Similarly, TMT labeling can also be used in proteomics to compare 6, 10, 11, and 16 sets of reporters for any peptide in different samples, since every peptide has an N-terminus and the TMT reagent can label the N-terminal amine and / or lysine, thereby attaching to every peptide.

[0072] Therefore, the present application provides an improved method for accurate, sensitive, and efficient identification and quantification of drug conjugates. In one aspect of the present invention, a drug-polypeptide conjugate, such as an ADC, is sequentially labeled with a first TMT, such as a Cys-reactive IodoTMT, and a second TMT, such as a TMT that reacts with N-terminal Lys or primary amines, and the labeled drug conjugate is then subjected to tandem mass spectrometry analysis. This sequential labeling scheme is useful for investigating drug conjugates. According to an embodiment of the present application, two parallel sample preparation arms can be used to label the drug conjugate (conjugate sample) and the unconjugated mock (reference or control sample). Both labels facilitate encoding unique reporter ion signatures that distinguish the sample and the mock or reference. The reference sample generates a reference reporter ion channel, and the conjugate sample generates a sample reporter ion channel. Unique reporter ions are generated after dissociation via either electron-based dissociation, such as HCD or ETD. The number of channels of reporter ion regions that implement specificity associated with the TMT, their ratios, and / or other mass analysis can be used to create unique barcodes for characterizing drug conjugates. For example, the barcodes can include distinct sets of reporter ions and / or mass barcodes.

[0073] As shown in Figure 3A, the method of the present application can be used to quantify the occupancy of a drug in a conjugate, for example, by comparing the mass spectrum of the lower m / z region reporter ion intensity of a Cys-containing peptide in the conjugate with the mass spectrum of a mock control containing a polypeptide not conjugated to a drug. This method can also be used to normalize the mock control and the conjugate.

[0074] Another aspect of the present application relates to a method for localizing a conjugation site in a polypeptide, for example, by using one or two TMT reporter ions to trigger an additional dissociation step to further characterize a peptide covalently bound to a drug. In one embodiment of the present application, one or two TMT reporter ions are generated using a first tandem mass spectrometry analysis, such as an HCD analysis. The reporter ions are then used to trigger an ETD scan of the peptide to obtain an ETD spectrum. The ETD spectrum complements the HCD spectrum for characterizing the conjugate, as the ETD provides sequence ions that aid in the localization of residues in the drug conjugate. Alternatively, the reporter ions are then used to trigger an ECD scan to obtain an ETD spectrum for further analysis of the peptide.

[0075] Figures 3A-3C show a dual TMT workflow using IodoTMT6 and TMT6 to label mAbs and their drug conjugates. In particular, IodoTMT6 is used to multiplex six different samples (e.g., cell lysates). In this case, labeling can be performed on proteins before digestion or on cysteine-bearing surrogate peptides after digestion of each sample. Both the iodoTMT6 and TMT6 reagents have reporters with the same nominal mass. Isobaric labeled peptides are generated for the selection of non-overlapping mass reagents. First, sequential labeling of the mAb with iodoTMT6 is performed, followed by trypsinization and labeling of the peptides obtained by tryptic digestion with the TMT6 label. Peptides from the conjugate sample and the mock are mixed in an equimolar ratio and subjected to LC-MS. The sequential labeling scheme described herein is a novel methodology for interrogating peptides, which facilitates the creation of mass spectrometry barcodes according to embodiments of the present application.

[0076] In certain embodiments, the illustrated workflow has two parallel sample preparation arms for drug conjugates and unconjugated mimics. For example, both IodoTMT6 and TMT6 labels facilitate encoding unique reporter ion signatures that distinguish between samples and mimics or references, e.g., a reference sample generates a reference reporter ion channel, and a conjugated sample generates a sample reporter ion channel. Additionally, the number of channels in the reporter ion region and their ratio encode the occupancy and type of experimental measurement that can be obtained, with each experimental measurement having a different set of reporter ions or mass barcodes.

[0077] Thus, aspects of the present application relate to a composition comprising a mixture of peptides labeled with at least one of a first TMT and a second TMT, and optionally one or more unlabeled peptides, wherein the first TMT and the second TMT do not have the same reporter ion mass, and the mixture of peptides comprises at least one peptide conjugated to a drug and labeled with at least one of the first TMT and the second TMT. For example, the composition of the present application may comprise a mixture of peptides derived from a conjugate sample labeled with at least one of the first TMT and the second TMT, and optionally one or more unlabeled peptides. The composition may also comprise a mixture of peptides derived from a conjugate sample described herein, peptides derived from a mimic labeled with at least one of a third TMT and a fourth TMT, and optionally one or more unlabeled peptides.

[0078] Figure 3A shows drug conjugation at a single cysteine ​​found in a surrogate tryptic peptide, where prior to digestion, iodoTMT labeling is performed on the free thiol of the mAb on the unconjugated fraction. Sample preparation and LC-MS can remain the same regardless of the number of conjugation sites. Data-dependent MS 2 or data-dependent SPS-MS 3(Synchronous precursor selection and triple-stage mass spectrometry) and TMT reporter ion-triggered ETD-MS can be performed in a seamless manner under full control of the instrument software used to generate mass spectrometry barcodes. This novel, automated, three-step method is referred to herein as "triple play," in which specific reporter ion combinations are used to quantify, normalize, and detect drug-polypeptide conjugates. In some embodiments of the present application, triple play is performed using dual TMT reporters to quantify drug occupancy, normalize drug conjugates in multiple samples, and detect or localize conjugation sites (e.g., by triggering an additional MS2). Preferably, the drug-polypeptide conjugate is an ADC.

[0079] Referring to Figures 3A-3B, following the workflow shown in the figures, all peptides bearing a single cysteine ​​conjugation site labeled with the iodoTMT labeling set generate four reporter ions (i.e., IodoTMT-126, IodoTMT-127, TMT-129, and TMT-130), while all non-cysteine-labeled peptides generate two reporter ions, TMT-129 and TMT-130, which can be used to correct for differences in sample mixing and normalization. The single reporter, TMT-130, uniquely found in drug-conjugated peptides can be used for additional ETD-MS analysis for site localization. 2 Scans can be triggered (see, for example, Figure 3B). The TMT-130 reporter ion is drug-independent, conjugated to a peptide and independent of drug fragments. As a result, triggered MS 2 can be used to screen libraries of drugs for any kind of drug conjugate or with unique reporter ions to easily identify and localize the drug.

[0080] Unlike peptides with a single cysteine, peptides with multiple cysteine ​​residues can have drugs covalently attached to one or more of the cysteine ​​residues. For example, the two hinge cysteines of IgG1 and IgG4 mAbs allow for dual and single occupancy of any single cysteine. The four hinge cysteines on IgG2 (with IgG2-A, IgG2-B, and IgG2-A / B isoforms) further increase the possibilities for combinatorial conjugation (Liu et al., MAbs 4, 17-23 (2012)).

[0081] Methods according to embodiments of the present application can be used to study drug conjugates with one or more drugs (such as antibodies) conjugated to a polypeptide. The one or more drugs can be within a single digested peptide. The drug molecules can be of the same or different types. Figures 3B-3D illustrate the study of drug conjugates containing one, two, or three drugs within a single digested peptide, where D1, D2, and D3 can be identical or unique. The number of barcodes for quantification and normalization remains constant regardless of the number of available conjugation sites and whether they are fully or partially conjugated. The occupancy in such examples is the total drug occupancy of all positional isoforms, and the number of barcodes for normalization remains at two reporters. Furthermore, when the number of conjugation sites within a single peptide is greater than two per peptide, partial conjugation generates two reporters, e.g., TMT-127 and TMT-130 ions, while full conjugation creates a single reporter, e.g., TMT-130 ion, to trigger ETD-MS2 scans for occupancy studies to localize the conjugated drug to the appropriate amino acid residue. As used herein, a peptide having "full conjugation" to a drug or "fully conjugated" to a drug refers to a peptide in which the drug is covalently bound at all amino acid residues available for conjugation. As used herein, a peptide having "full conjugation" or "not fully conjugated to a drug" refers to a peptide in which the drug is covalently bound at only some, but not all, amino acid residues available for conjugation. A peptide that is fully conjugated to a drug may have one, two, three, or more drugs conjugated to it.

[0082] While the sample preparation workflow remains the same for analyzing such conjugation reactions, the mass spectrometry parameters for TMT triggering require either one of two reporters or two of two reporters to be included in the trigger setup for localization of fully multiplexed or partially multiplexed conjugations. Using a similar method / scheme, and given the present disclosure, drug conjugates containing four or more drugs can be studied using the methods of the present application.

[0083] Figure 4 shows some exemplary experimental ADC systems analyzed by the methods of the present invention. For example, the NIST mAb-biotin PEO acetamide ADC has biotin PEO acetamide conjugated to the mAb and its mock control, which contains only the antibody without conjugated drug. In addition to antibodies or fragments thereof, drug conjugates with other polypeptides can also be characterized by the methods of the present application. Human serum albumin (HSA) peptide is used as an example. Unlike NIST, which has only one peptide with two cysteines, the HSA peptide has multiple cysteines on the same peptide. It was used to test methods according to embodiments of the present application, such as fluorescence assays and IodoTMT labeling.

[0084] In the representative structure shown in Figure 4, two biotin PEO acetamides are conjugated to the NIST mAb, one in the light chain and one in the heavy chain of the NIST mAb. However, a NIST mAb-biotin PEO acetamide ADC may have several different isoforms with different combinations of biotin PEO acetamide present and attached to different cysteine ​​residues within the NIST antibody with different levels or occupancies. Similarly, the representative structure of MSQC8 shown in Figure 4 has only one dansyl fluorophore conjugated to the mAb (MSQC4), but can have different isoforms of the dansyl fluorophore conjugated to the mAb at one or more other sites.

[0085] Any ADC, including but not limited to any of the ADCs shown in Figure 4, and its mock controls, can be subjected to tandem mass spectrometry analysis according to embodiments of the present application having a dual TMT, such as a Cys-reactive IodoTMT TMT, and a TMT reactive to N-terminal Lys or primary amines. Examples of mass spectra obtained from this analysis, as well as results from the analysis, are shown, for example, in Figures 5A, 5B, 5C, 5D, 6A, 6B, 7, and 9A-9D.

[0086] In another embodiment, three different types of reporter barcodes are used to calculate the drug occupancy at each cysteine ​​residue. Figure 5A shows HCD-MS analysis of dual TMT-labeled isobaric peptides of the NIST mAb light chain. 2 The spectra are shown. The peptide has unoccupied cysteine ​​residues labeled with iodoTMT (io) and N- and terminal lysine residues labeled with TMT (TMT). The HCD spectrum (left panel) consists of a series of characteristic b- and y-type backbone product ions localizing the labeling sites with the corresponding TMT labels. The lower mass range reveals four reporter ions, which are more clearly shown in the expanded right panel, including the reporter ion masses of each dual TMT channel: iodoTMT-129 and TMT-128 pair representing the simulated channel, and iodoTMT-126 and TMT-130 pair representing the conjugate sample channel. Occupancy can be derived either by using the iodoTMT intensities of the simulated (A2) and conjugated (A1) or the TMT intensities of the simulated (B2) and conjugated (B1). The HCD spectrum provides both sequence ions and spectral ratios to identify the site conjugation at light chain Cys-193 with biotin-PEO-acetamide at approximately 50% occupancy. Similarly, occupancies can be obtained for given reaction conditions for conjugate generation. For example, Figure 5B shows reporter ion regions for two additional sites: light chain Cys-63 with approximately 65% ​​biotin-PEO-acetamide occupancy and heavy chain Cys-147 with approximately 100% biotin-PEO-acetamide occupancy, and Figures 5C and 5D show the HCD-MS results. 2 The corresponding MS of peptides sequenced by2 The spectrum (with all backbone product ions) is shown.

[0087] The dual TMT workflow uses a normalization factor between the conjugate and mock samples to correct for deviations in occupancy estimates. For example, Figures 6A and 6B show HCD-MS results of dual TMT-labeled isobaric peptides of the NIST mAb heavy chain. 2 The spectra are shown. The N- and C-terminal lysines of the peptide are labeled with TMT. The HCD spectrum (Figure 6A) consists of a series of characteristic b- and y-type backbone product ions localizing the TMT labeling site. The lower mass range shows two reporter ions, the reporter ions TMT-128 and TMT-130 (Figure 6B), representing the mock and conjugate sample channels, respectively. Peptides lacking cysteine ​​residues exhibit such a characteristic mass barcode of two reporter ions that the reporter ion intensities represent the concentrations of the mock and conjugate samples. Typically, a ratio of 1 is observed for such fully labeled non-cysteine ​​peptides.

[0088] In another embodiment, the sequence ions and immonium ions of the corresponding drug fragments are useful for determining the site of site occupation of the conjugated peptide (see, for example, Figure 7). When multiple potential conjugation sites exist on the same peptide, it is difficult to determine the site location using conventional methods. Site-specific sequence ions often do not exist, and peptide localization is difficult when the mass-to-charge ratios of peptide sequences are indistinguishable. As a result, determining the precise location of the conjugation site often requires site-specific sequence ions obtained by additional complementary dissociation methods known in the art. For example, ETD-MS2 is complementary to HCD-MS2. Two methods can be used to generate sequence ions that can distinguish sites, or in other words, localize modified residues. For example, drug-conjugated cysteines can be localized from unbound cysteines. Methods according to embodiments of the present application make it possible to determine the site occupation of conjugated peptides, including those with multiple conjugation sites, without using complementary dissociation methods. For example, dissociation can be performed to identify and localize payloads.

[0089] The methods of the present application may further include steps to improve mass triggering, quantification sensitivity, and accuracy using methods known in the art in view of this disclosure. For example, HCD-MS 2 can be performed on mass-selected precursor ions of TMT-labeled peptide conjugates in the ion-routing multipole (see, e.g., Figures 8A and 8B). The resulting ion spectrum is acquired in the Orbitrap where the TMT reporter ions are detected. The reporter ion mass (130.14 Da in Figure 8A) can be used to trigger mass selection of the same precursor ions and transfer them into the ion trap. Subsequently, ETD-MS 2 The ion trap is then used to perform mass analysis of the resulting product ions in the Orbitrap. For example, Figure 8B shows the MS, MS of the NIST light chain cys-193 biotin PEO acetamide conjugated peptide. 2 , and Trigger MS 2Figure 1 shows the TICs and corresponding mass spectra for data-dependent scans occurring sequentially from 1 to 3. The TIC labeled 1 is the full MS scan collected in the Orbitrap. The TIC labeled 2 is the HCD-MS of mass-selected precursor ions acquired in the Orbitrap in two consecutive scans. 2 and the TIC labeled with 3 was obtained within the same scan period and mass analyzed by the Orbitrap TMT-130 triggered ETD-MS. 2 Both MS spectra are 2 The time penalty incurred for high-resolution Orbitrap mass analysis sequencing of product ion spectra is likely to have no effect on the relatively small subset of drug-conjugated peptides.

[0090] Charge-loss ions generated by HCD-MS2, such as the reporter ion TMT-130, can be used to trigger ETD-MS2 and generate ETD spectra. Figures 9A and 9B show annotated HCD and ETD spectra, respectively, for a Cys-193 biotin-PEO-acetamide conjugated peptide. It is important to note that the highly selective TMT130 ion and immonium ions from the drug are readily observed in the spectrum. In addition, the characteristic neutral loss of the reporter and the entire tag were observed in the ETD spectrum, indicated by asterisks in Figures 9B and 9D. The backbone fragments from both ETD and HCD are complementary and localize the biotin-PEO-acetamide on Cys-193. Despite not being the most abundant immonium ion, TMT130 has been shown to be a useful immonium ion. For example, Figures 9C and 9D show trigger mass detection of the peptide-compatible biotin-PEO-acetamide conjugated at Cys-23, where TMT130 was less abundant compared to the immunoion generated by biotin-PEO-acetamide. The specificity of generating the TMT130 mass trigger can also be controlled to interfere with peptides by reducing the mass selection window of the precursor ion.

[0091] For example, co-isolation and co-fragmentation of peptides allows for ETD-MS on ADC-peptides to localize conjugation sites on polypeptides. 2 This can result in a loss of specificity for the TMT-130 reporter ion, which serves to trigger the analysis. Using a higher trigger intensity threshold or a narrower isolation window (e.g., 0.4 Da) can improve mass triggering (see, e.g., Figures 10A and 10B). Different reporter ions can affect the type of barcode used for triggering, particularly co-isolation and co-fragmentation due to peptides co-eluting with the same isolation window size (e.g., 2 Da) (Figure 10B).

[0092] In another embodiment, SPS-MS 3 Using HCD-MS, the specificity and accuracy of detection and quantification can be further improved (see, for example, Figure 11). Precursor ions are dissociated by collision-induced dissociation (CID) in the ion trap, and several product ion masses are synchronously isolated using a notch wave configuration applied to the ion trap. The isolated product ions are then analyzed by HCD-MS. 3 The ions are then transferred to an ion-routing multipole where fragmentation is carried out. SPS reduces any interference from co-fragmentation during standard HCD, resulting in less interfering reporter ions.

[0093] For example, synchronous precursor selection was used in reporter quantification studies on ADCs of MSQC8, which has the structure shown in Figure 4, and its mock control MSQC4, an antibody without conjugated drugs, using methods according to embodiments of the present application. As shown in Figures 12A-12C, application of synchronous precursor selection allowed for accurate TMT reporting, thereby improving quantification sensitivity and accuracy. Accurate reporter ion ratios were obtained using SPS-MS. 3Later, N = 5 ions were observed with the iodoTMT-128, TMT-129 reporter ion pair representing MSQC8 and the iodoTMT-130, TMT-131 reporter ion pair representing MSQC4 (simulant). The reporter ion ratio of MSQC8:MSQC4 was 1, indicating that the cysteine ​​residues were unconjugated.

[0094] Figures 13A, 13B, and 14 show experiments and results using dual TMT to quantify site-specific ADCs on MSQC8 using a method according to an embodiment of the present application. As shown in Figures 13A and 13B, when ADC labeling was incomplete—for example, the conjugate was labeled only with Cys-reactive TMT, not Lys-reactive TMT—the detected occupancy (% ADC) was slightly lower than that with complete labeling. Interestingly, despite finding peptides with incomplete labeling, similar site occupancy rates of 55–60% were observed, demonstrating the robustness of the present application's method.

[0095] Preferably, the conjugate is fully labeled with both TMTs. Labeling completion can be measured by the intensity of the report ions for the TMTs in the mass spectrum. The results shown in Figures 13C-13F show that in MSQC8, the drug is conjugated to the antibody at Cys-266 and Cys-372. Cys-218 is the only conjugation site for dansylcadaverine-SMCC on the light chain, with a site occupancy of 60%, which is also closely consistent with the average DAR of 1 observed by SLIM-IMS (Nagy et al., Anal Chem 92, 5004-5012 (2020)) and the reduction in mass spectrometry of MSQC8 (data not shown). A maximum of 60% occupancy at Cys-266 and 15% at Cys-372 were estimated, while all other cysteines showed 0% conjugation (see, e.g., Figures 14A-14C). Such results are consistent with the 1:2 DAR0 / DAR1 ratio obtained from existing methods, such as structure for lossless ion manipulation combined with ion mobility spectrometry (SLIM-IMS) of the light chain. Furthermore, the use of multiple peptides to normalize for differences in mixing between the two samples (i.e., the MSQC8 ADC sample and the MSQC4 simulant) ensures that occupancy estimates are accurate for each conjugation site.

[0096] Methods according to embodiments of the present application can also be used to characterize or identify the structure of drugs conjugated to polypeptides. As shown in Figure 15, small molecule conjugates are prone to fragmentation, generating complex spectra. The fragment masses from drugs are specific to each drug and can be used to identify the drug. Figure 15 shows a series of immonium ions for the SigmaMAb dansyl-cadaverine-SMCC conjugate, which are significantly different from the immonium ion of biotin PEO acetamide. Similar to the results in Figures 8A and 8B, by using specific reporter ion m / z to trigger ETD, the method of the present invention provides ETD-MS analysis that is independent of the structure of small molecule drugs that tend to generate fragments. 2 In other words, the TMT reporter m / z is specific, but the reporters from drugs that alter m / z depend on the structure of the molecule.

[0097] According to other embodiments of the present application, dual TMT mass spectrometry according to the present invention can also be used to profile the reaction time course of conjugation reactions (see, e.g., Figures 16A-16B, 17A, and 17B). In the dual TMT experimental scheme of Figures 16A and 16B, a single TMT was used with synthetic peptide standards (such as HSA peptide) with different numbers of cysteine ​​residues for multiplexed reaction time course experiments combining TMT with fluorescence.

[0098] In a further embodiment of the present application, dual TMT mass spectrometry according to the present invention can be used for multiplexed quantification of drug-polypeptide conjugates to increase throughput and reduce costs. Figure 18A shows a multiplex format in which an additional drug-conjugate sample is added to the original workflow. Multiplexing allows multiple reaction conditions to be monitored in a single analysis, which is advantageous over separate workflows for each sample. Careful selection of TMT reagents with non-overlapping reporter ion masses enables even higher-order sample multiplexing. Multiplexing also alters the reporter ion signature for occupancy, where the number of reporters in the barcode is (2n + 2) relative to the number of samples, n. In the example of Figure 18A, the addition of a second sample results in a total of six reporters, and these reporters are derived from equal numbers of IodoTMT and TMT reagents. The number of reporter ions in the barcode for normalization is half the number as the barcode for occupancy, i.e., (2n + 2) / 2. This is a dual TMT strategy that always performs normalization across samples using only amine-reactive TMT reporter ions. It is important to note that the number of reporter ions in a barcode for a peptide-drug conjugate mass trigger does not depend on the number of samples. Rather, the number of reporters for a mass trigger depends on the number of possible conjugation sites, m. Typically, when m = 1, a single reporter is observed; when m > 1, the drug is not conjugated at all sites, and two reporter ions are observed.

[0099] In certain embodiments of the present application, multiplexed triplex assays can be used to analyze multiple samples containing conjugates with two or more conjugation sites. For example, synthetic peptides with one to three cysteine ​​residues conjugated to the fluorescent molecule DCAM-3 are analyzed using the methods of the present application. Figure 16B shows the results of iodoTMT in combination with IAA, which blocks unreacted cysteines. 6 This application demonstrates the use of the method of the present application to monitor the time point of a reaction using IodoTMT. 6 is TMT because the upper limit of multiplexing is 4 under the assay conditions.10 Currently, TMT has not been used as a dual-labeling approach in conjugation with 16 The availability of future n-plex reagents (16-plex) and n>16 greatly facilitates the number of samples for dual TMT approaches. Fluorescence-based quantification was used as the standard and compared with TMT-based occupancy estimation. Figure 16C shows the triple-play workflow of this application applied to monitoring reactions of multiple drugs: D1–D5 in a multiplexed fashion. This scheme represents TMT labeling with IAA or iodoTMT to achieve dual labeling. Occupancy can be estimated for each drug using reporter intensities, which indicate a rank order of reactivity: D5>D3>D1, and for failed reactions of D2 and D4 with zero occupancy. Figure 16D shows mass triggering via a single TMT reporter ion specific for each drug molecule: TMT127 for D1, TMT129 for D2, and TMT130 for D3.

[0100] Figures 17A and 17B show the results of a multiplexed experiment in which reaction time points were simulated by mixing peptide-DACM-3 conjugation percentage ratios of 0, 20, 40, 60, and 80. Each peptide mixture was prepared separately so that the unconjugated peptide counterpart was first reduced and all free thiols were blocked with IAA. The conjugated peptide mixture and the unconjugated counterpart of each peptide sequence were used in two parallel experiments. First, fluorometric measurements were performed to ensure DACM conjugation and mixing. The fluorescence intensity of all peptides with one to three cystine residues shows a linear response from no conjugation (mock) to 80% conjugation. Next, five peptide-DACM conjugate ratios of 0, 20, 40, 60, and 80 and each sample were labeled with iodoTMT 126, 127, 128, 129, 130, and 131, respectively, and equimolar amounts were mixed for reporter ion quantification. The HCD-MS2 reporter ions reflect a unique barcode for the reaction, which plateaus as reactants are depleted as the reaction progresses. The reporter intensity of the peptide mixture relative to its mimic decreases with increasing conjugation, as expected. Correlations of observed conjugation levels with expected or theoretical conjugation levels were obtained based on the TMT reporter ions. It is important to note that TMT reporter ion intensity is inversely proportional to conjugation level. In particular, the dynamic compression of the TMT ratio relative to low-intensity reporter ions most impacts high-drug conjugates.

[0101] Algorithms such as those reported by Savisky et al. can be used to correct for ratio compression (Savisky et al., 2013, Journal of proteome research 12, 3586-3598), the entire contents of which are incorporated herein by reference. SPS-MS3 routines available with current instrumentation can also improve ratio compression (McAlister, et al., Anal Chem 86, 7150-7158 (2014)). Nevertheless, the present method, without any correction, can provide accurate estimates of low levels of conjugation, where the TMT response is a linear function of conjugation. Detecting low conjugate stoichiometry is beneficial because most off-target conjugation is undesirable and can be more accurately monitored. Multiplexed TMT ratios and / or reverse TMT ratios (site occupancy) were compared with fluorescence-based yield estimates. The linear dynamic range for fluorescence-based measurements is higher compared to the dynamic range for TMT reporter occupancy due to ratio compression. However, TMT reporters with high intensity are also channels with low levels of conjugation, which can be measured with fewer ratio compression effects and missing values ​​(Lim et al., Journal of Proteome Research 16, 4217-4226 (2017)).

[0102] In another embodiment, the method of the present application includes using a robotic system. For example, the triple-play workflow of the present application can be implemented on the AsayMap Bravo liquid-handling robotic system, where dual labeling with TMT reagents and sample multiplexing can enhance the accuracy and precision of TMT-based quantification. Figure 18A shows an overall scheme where automation can be particularly beneficial for robust monitoring of reactions at the point of synthesis. Simultaneous analysis of multiple drug conjugation reactions can be useful when reaction conditions need to be rapidly optimized. Furthermore, estimation of drug occupancy at intermediate steps, in addition to the final product, is necessary for sequential conjugation reactions to optimize overall yield. The speed and reproducibility that any automated platform provides with multiplexing is necessary to reduce human error during sample handling.

[0103] In one embodiment, the sample multiplexing scheme of the present application can analyze up to four ADC samples using dual TMT labeling of samples such as MSQC8 at two different concentrations in duplicate. Figure 18B shows the TMT labeling such that all masses are unique. 10 Derived dual TMT reporter and IodoTMT 6 Select a reagent: TMT 10 (10-plex) reagents are labeled isotope-substituted or C 12 , N 15 Atom pair is C 13 , N 14 TMT differs by 6.32 mDa (millidaltons) and is substituted with 10 xN or TMT 10 It has six of the ten isotopes labeled as xC. 10 The xC isotopic masses are identical to the iodoTMT reagents and cannot be used simultaneously. When a sample is multiplexed with the appropriate combination of reporter ions, high-resolution mass spectrometry allows baseline separation of reporter ion masses and their isotopic masses. Four different ADC samples and a mock or unconjugated sample are dual-TMT labeled as shown in the scheme, whereby the cysteine-containing tryptic peptide is an isobaric mixture of five TMT reporters and five iodoTMT reporters. During MS2, the barcode consists of 10 reporter ions.

[0104] Four MSQC8 drug conjugate mimics were prepared at two concentrations: the original sample and another sample at half the concentration by diluting with MSQC4 and MSQC8. Each of these samples was a duplicate sample with dual TMT labeling. Figure 19 shows the reporter ion of the Cys-218 peptide after HCD-MS2. The reporter of the mimic (MSQC4) was TMT. 10 -126 and IodoTMT 6 -130 pairs were double-labeled, while four samples were TMT 10 xN, IodTMT 6The MSQC8 replicates were labeled with x, where x ranges from 127 to 130. Replicate 1 of MSQC8 was labeled with dual reagent x = 127, and replicate 1 of MSQC8 was labeled with dual reagent x = 130. Similarly, the two replicates for the equimolar mixture of MSQC8 and MSQC4 labeling reagents are x = 128 and 129. The site occupancy at Cys-218 for the ith sample is given by Equation 1. Figure 20A shows the site occupancy of five TMTs during MS2-HCD. 10 Figure 20B shows the use of non-cysteine ​​peptide sequences to correct sample concentrations in multiplexed experiments with barcodes of reporter ions (one mock and four samples). The normalization factor for the i-th sample is given by Equation 2. The corrected occupancy for each ADC sample can be obtained by Equation 3. Figure 20B shows the normalized occupancy obtained for four samples in a single acquisition. In principle, these multiplexed experiments can also be used to identify drug conjugates using trigger masses.

[0105] In one embodiment of the present application, the relative occupancy between any two reaction steps can be determined when the reaction proceeds through intermediate steps or when reference material is unavailable (see, e.g., Figures 20A and 20B). As shown in Figures 16A and 16B, occupancy can be estimated for a peptide conjugated to a fluorophore drug mimic, DACM-3, via a two-step antibody conjugation reaction. An example of a two-step antibody conjugation reaction is a transglutaminase reaction followed by click addition of a cytotoxic payload. See, e.g., Huggins, et al., Molecules. 2019 Sep;24(18):3287, the contents of which are incorporated herein in their entirety. Any ADC prepared by a two-step antibody conjugation reaction can be analyzed by the methods of the present application.

[0106] The present invention will now be described in more detail with reference to the following specific, non-limiting examples. Those skilled in the art will appreciate that the techniques disclosed in the examples which follow represent techniques discovered by the inventors to function well in the practice of the invention, and can therefore be considered to constitute preferred modes for its practice. However, those skilled in the art will, in light of the present disclosure, appreciate that many changes can be made in the specific embodiments which are disclosed and still obtain like or similar results without departing from the scope of the invention. [Example]

[0107] Example 1: Conjugation of dual TMT-labeled and unconjugated mAb Four different ratios (1:1, 1:9, 9:1, and 1:3) of reduced and drug-conjugated NIST monoclonal antibody (mAb, Sigma Aldrich, catalog number 8671) (sample #1) to non-reduced and IodoTMT™-tagged NIST mAb (sample #2) were prepared. NIST mAb was conjugated using either iodoacetamide (IAA) (Sigma Aldrich, catalog number 16125) or biotin-PEO iodoacetamide (Sigma Aldrich, catalog number B2059). For sample #1, two aliquots of 100 μg of NIST (50 μl of 2 mg / ml NIST) were prepared by combining 10 μl of the stock (10 mg / ml) with 40 μl of a solution containing 8 M guanidine hydrochloride (GuHCl) (Sigma Aldrich, catalog number G3272) and 4 mM ethylenediaminetetraacetic acid (EDTA) (Sigma Aldrich, catalog number 03620). The NIST aliquots were then reduced by adding 10 μl of 1 M DTT (Sigma Aldrich, catalog number 1019777001) and incubated at 37°C for 1 hour. Alternatively, TCEP can be used as a reducing agent instead of DTT. DTT is typically active in neutral to basic pH conditions, while TCEP has a wider pH range and is a stronger reducing agent.

[0108] NIST was conjugated with 1M IAA. 1M IAA was prepared by adding 300 μl of trimethyl ammonium bicarbonate (TEAB) (ThermoFisher, part of the TMT labeling kit) to an Eppendorf tube, which was vortexed and sonicated for 20 minutes to allow the TMT reagent to equilibrate to room temperature before use. 24 μl of 1M IAA was then added to the reduced NIST sample, which was incubated in the dark at room temperature for 1 hour. Following the incubation period, 15 μl of 1M DTT was added to the sample to quench the conjugation reaction. The sample was combined with a buffer exchange solution (8M GuHCl + 4M EDTA) and placed through a Zebra™ spin desalting column with a molecular weight cutoff of 7 kDa (ThermoFisher, catalog number 89882).

[0109] To conjugate the reduced NIST sample with biotin PEO iodoacetamide, the sample was buffer exchanged into sulfhydryl-free PBS (phosphate buffered saline) at a pH of approximately 7.5. A 20 mM stock solution of biotin PEO iodoacetamide was prepared immediately before use (190 μl of PBS was added to 2 mg of biotin PEO iodoacetamide in an Eppendorf tube). The reduced NIST sample in PBS was combined with 5 μl of 20 mM biotin PEO iodoacetamide and mixed. The reaction was incubated on ice or at room temperature for 2 hours. Following the incubation period, the sample was desalted. The sample was placed in buffer exchange solution (8 M GuHCl + 4 M EDTA) and passed through a Zebra™ spin desalting column with a 7 kDa molecular weight cutoff.

[0110] Sample #2 (IodoTMT™-tagged non-reduced NIST sample) was prepared by generating three aliquots of NIST (50 μl of 2 mg / ml NIST combined with 10 μl of stock (10 mg / ml)) and adding them to 40 μl of solution (8 M GuHCl + 4 mM EDTA). Each aliquot was combined with 50 μl of a solution containing 8 M GuHCl and 4 mM EDTA to offset its volume. To reduce the sample for subsequent iodoTMT labeling, 10 μl of DTT was added to each aliquot, and the samples were incubated at 37° C. for 1 hour. Four ratios of Sample #1 and Sample #2 were prepared: 1) a 1:9 ratio (10 μl reduced NIST:90 μl non-reduced NIST), 2) a 1:3 ratio (25 μl reduced NIST:75 μl non-reduced NIST), 3) a 1:1 ratio (50 μl reduced NIST:50 μl non-reduced NIST), and 4) a 9:1 ratio (90 μl reduced NIST:10 μl non-reduced NIST). A sample mixture containing 100 μg of NIST and 2 μl of IodoTMT™-LabelA1 (10 μl methanol added to 200 μg IodoTMT™) was added to all samples. The samples were then incubated in the dark at 37° C. for 1 hour. To quench the labeling reaction, 4 μl of 0.5 M DTT was added to each sample and incubated for an additional 15 minutes at 37°C in the dark. Samples had their buffer exchanged with TEAB, and IodoTMT™-labeled proteins were digested with 4 μl of trypsin (1 mg / ml) for 4 hours to overnight at 37°C. Trypsin was quenched with 2 μl of 98% formic acid, and 2 μl of TMT-LabelB1 (800 μg dissolved in 40 μl of anhydrous acetonitrile) was added to each of four samples. Samples were incubated for 1 hour at 37°C in the dark, and the reaction was quenched by adding 8 μl of 5% hydroxylamine for 15 minutes at room temperature.

[0111] In addition to Sample #1 and Sample #2, a reference or mock sample, designated Sample #3, was prepared and tagged with IodoTMT™ tags. Sample #3 was prepared using the same protocol as Sample #2, except that four samples were generated instead of three. Sample #3 was separately trypsinized and labeled with TMT-LabelB2. Samples #1 and #2 were each mixed with 132 μl of Sample #3 in a 1:1 volume ratio, resulting in TMT-labeled proteins with unique isobaric reporter ion masses forming four multiplexed samples for mass spectrometry analysis. Up to four reaction mixtures can be multiplexed by dual labeling with IodoTMT™ and five unique reporter masses from the amine-reactive TMT10plex™ reagent (ThermoFisher, catalog no. 90110). The four dual-labeled conjugate samples and the single mock / reference control sample were combined in a volume ratio of 1:1:1:1:1, which generated a single multiplexed sample for liquid chromatography-mass spectrometry analysis.

[0112] 13A, 13B, and 14 show experiments and results using dual TMT to quantify site-specific ADC on MSQC8 using a method according to an embodiment of the present application. As shown in FIG. 13A, when ADC labeling was not complete, for example, when the conjugates were labeled with only Cys-reactive TMTs, e.g., IodoTMT128 and IodoTMT130, but not with Lys-reactive TMTs, e.g., TMT129 and IodoTMT131, for MSQC-4 and MSQC-8, respectively, the detected site occupancy (e.g., 55-60% ADC) in single labeling was slightly less than that in dual labeling. Dual labeling of unconjugated peptides with IodoTMT128 and TMT129 for MSQC-4 and IodoTMT130 and TMT131 for MSQC-8 provides a 60% more accurate estimate (measured from two reporters instead of one). Labeling completion was measured by the intensity of the TMT reporter ions in the mass spectrum. MSQC8 was known to have several conjugation sites. The results shown in Figure 13B indicate that in MSQC8, the drug was conjugated to the antibody at Cys-266 and Cys-372. Cys-218 was the only conjugation site for dansyl-cadaverine-SMCC on the light chain with a site occupancy of 60%, closely matching the average DAR of 1 observed by SLIM-IMS (e.g., Nagy, G. et al., Anal Chem 92, 5004-5012 (2020)), which found drug conjugation to both chains of an antibody. Furthermore, mass spectrometric reductions in MSQC8 occupancy of 60% (maximum) and 15% were estimated for Cys-266 and Cys-372, respectively, while all other cysteines showed 0% conjugation (see, e.g., Figure 14). Such results are consistent with the 1:2 DAR0 / DAR1 ratio obtained from existing methods, such as the structure for lossless ion manipulation coupled with ion mobility spectrometry (SLIM-IMS) of the light chain.It is important to note that using multiple peptides to normalize for differences in mixing between the two samples (i.e., MSQC8 ADC sample and MSQC4 simulant) ensures that occupancy estimates for each conjugation site are accurate.

[0113] Example 2: Dual TMT labeling of conjugated mAb Conjugated mAbs were labeled using a dual TMT labeling protocol. MSQC8 (Sigma-Aldrich mAb antibody-drug conjugate mimic) and MSQC4 (Sigma-Aldrich mAb standard) were used as the conjugated sample and reference or mock sample, respectively. Samples were tagged with separate IodoTMT™ tags according to the sample protocol used to tag the NIST mAb and separately trypsinize to generate peptides. The resulting peptide mixtures from MSQC8 and MSQC4 were separately labeled with separate amine-reactive TMT tags according to the same protocol used to label the NIST mAb with TMT or IodoTMT™. After TMT dual labeling, the samples were mixed at a 1:1 volume ratio, generating a single sample that was multiplexed for mass spectrometry analysis.

[0114] Example 3: Automation of TMT labeling in AssayMap Bravo The double TMT labeling protocol was implemented on an AssayMAP Bravo (Agilent) robotic system, which automates protein sample preparation before analysis by LC-MS. Double TMT labeling of unconjugated NIST mAb conjugates was performed as previously described. An in-solution digestion single-plate protocol was used according to the manufacturer's instructions, which are incorporated herein by reference in their entirety (see worldwide web: agilent.com / cs / library / applications / application-protease-digestion-in-solution-assaymap-5994-1682en-agilent.pdf). The automated sample preparation was programmed to dispense a minimum volume of 5 μl, and samples were desalted by reverse-phase protein cleanup using an RP-W cartridge application known in the art. Different sample ratios (e.g., 1:1, 1:9, 9:1, and 1:3) were performed using the AssayMAP Bravo robotic system's reformat utility.

[0115] Example 4: Multiple TMT labeling of conjugated peptides Synthetic mAbs bearing one to three cysteine ​​residues were separately conjugated with N-(7-dimethylamino-4-methylcoumarin-3-yl)maleimide (DACM-3; ThermoFisher, catalog number D10251) via an adapted manufacturer's protocol for protein labeling. A stock solution of 16.76 mM DACM-3 was prepared. 1 mg of each peptide was dissolved in 1 mL of a solution containing 100 mM PBS, 0.1 M NaCl, 10 mM EDTA (pH 8.0), and 50 μL of 16.76 mM DACM-3. The peptides were sealed in a lightweight protective cover and incubated at ambient temperature for approximately 5 minutes. Mass spectrometry analysis was used to confirm complete peptide conjugation. The relative fluorescence (relative fluorescence units or RFU) of samples of N-acetyl-L-cysteine ​​(a cysteine ​​peptide standard derived from a NIST monoclonal antibody synthesized to 99.99% purity by Biomatik Corporation) and standard curve samples was measured (excitation = 385 nm, emission = 465 nm, 455 nm cutoff) using a fluorescence plate reader (Spectramax M5, Molecular Devices). The relative concentration of free or conjugated thiol for each peptide was calculated from regression analysis of the internal N-acetyl-cysteine ​​curve. N-acetyl-L-cysteine ​​was determined to be essentially 100% reactive with DACM-3.

[0116] Following DACM-3 conjugation of the peptides, the peptides were labeled with TMT. Each DACM-3-conjugated peptide was mixed with its unlabeled counterpart to generate five mixtures at various stoichiometric ratios (0, 0.2, 0.4, 0.6, and 0.8). The unlabeled peptide served as a control. Samples were evaluated with fluorescent assays known in the art in light of this disclosure to ensure that the DACM-3 labeling functioned properly prior to TMT labeling. Amine-reactive 6-plex TMT labeling was performed on each sample as described above for TMT labeling of tryptic peptides. Finally, the five labeled mixtures and the control were each combined in a 1:1 volume ratio and analyzed by LC-MS using methods known in the art in light of this disclosure.

[0117] Example 5: LC-MS 2 (LC-MS / MS) analysis LC-MS 2 Samples analyzed in were separated on an Agilent Infinity 12900 UHPLC using an AdvanceBio Peptide Mapping column (Agilent, catalog number 864600-911) at 65°C. A 50-minute liquid chromatography (LC) gradient program using LC-MS grade water with 0.1% formic acid as mobile phase A and acetonitrile as mobile phase B was performed according to the following protocol: 0 min, 2% B; 35 min, 30% B; 40 min, 80% B; 45 min, 85% B; 45.5 min, 2% B; and 5 min, subsequent re-equilibration at 2% B. The flow rate was set at 0.2 mL / min, and the injection volume was set at 2 μl. The mass spectrometer was operated using data-dependent (dd) MS techniques known in the art. 2 HCD (MS / MS-HCD) and electron transfer dissociation (ETD) were operated in positive ionization mode with the following interface conditions: emitter voltage +2600 V; vaporizer temperature 325 °C; ion transfer tube temperature 325 °C; sheath gas 55 (arbitrary units); auxiliary gas 10 (arbitrary units); and sweep gas 1 (arbitrary units).

[0118] The following internal mass spectrometer settings were used for the following MS scans: RF (radio frequency) lens, 60%; AGC (automatic gain control) target, 1e6; maximum injection time, 50 ms; 1 μscan in profile mode at 70K resolution on an Orbitrap (Orbitrap, OT) mass spectrometer. This method can be used with any MS known in the art. 2A series of filters were sequentially included before the HCD event. A monoisotopic peak selection filter was included and set as peptide for all methods, using a 1e5 intensity filter. Some methods used an optional charge state filter to select precursor charge states 2-6. Additionally, certain methods used an optional dynamic exclusion (DE) filter to more efficiently identify peptides in samples with either a 12-second or a 3-second exclusion window, exhibiting the following common parameters: n = 1 exclude; + / - 3 ppm; isotope excluded; and a single charge state per precursor. One method included Apex detection, which used the following parameters: expected peak width, 6 seconds; desired apex window, 30%.

[0119] 5ddMS 2 OT-HCD (Data-Dependent MS / MS-Orbitrap Detection-Higher Energy Collisional Dissociation) scans were performed with the following settings: quadrupole isolation, 1.6 m / z isolation window; detector type, Orbitrap, automatic m / z normal scan range, 70K resolution, 100 m / z first mass; AGC target, 2e5 inject ions at all available parallelizable time, maximum injection time of 50 ms; 1 μscan, profile. ddMS 2 Following OT-HCD, a targeted mass trigger (TMT) scan was performed, which is a scan that is triggered only when the system detects a product ion from a user-defined list. The target ion mass includes a TMT reporter ion specific to detecting the payload from a list of reporter masses (e.g., 126-131 Da). Only the ions with the most intense mass-to-charge ratios within the top 10 were used for all mass triggers. The following conditions were used for ddMS: 2 For OT-ETD (data-dependent MS / MS-Orbitrap detection-electron transfer dissociation), MS nLevel, 2; Quadrupole isolation, 1.6 m / z isolation window; ETD response time, 50 ms; Detector type, Orbitrap, automatic m / z normal scan range, 30K resolution; AGC target, 5e4, inject ions at all available parallelizable times, maximum injection time, 22 ms; 1 μscan, profile. ddMS 2 OT-HCD and ddMS 2 The number of dependent scans between OT and ETD was set to 1.

[0120] Data from the various mass spectrometry analyses were processed with Xcalibur™ data acquisition and interpretation software (ThermoFisher, catalog number OPTON-3096 7), MaxQuant and Perseus source proteomics data analysis software (MaxPlanck Institute), and R 3.6 statistical programming software (R Foundation for Statistical Computing, Vienna, Austria). Site occupancy was estimated by equation 1 using the IodoTMT reporter ion peak area, where A2 is the area of ​​the unconjugated sample and A1 is the area of ​​the conjugated sample. Normalization factors were calculated by equation 2 using the TMT reporter ions, where B1 is the peak area of ​​the conjugated sample and B2 is the peak area of ​​the unconjugated sample.

[0121]

number

[0122] It will be understood that the examples and embodiments described herein are for illustrative purposes only, and that modifications to the above-described embodiments may be made without departing from the broad inventive concept thereof. It is therefore understood that the invention is not limited to the particular embodiments disclosed, but that it is intended to cover modifications within the spirit and scope of the invention as defined by the appended claims. The inventions described in the original claims of this application are listed below. [Invention 1] 1. A method for analyzing a conjugate comprising a drug covalently attached to a polypeptide, comprising: (i) contacting a sample containing the conjugate with a first tandem mass tag (TMT), thereby labeling the polypeptide of the conjugate with the first TMT; (ii) digesting the polypeptide of the conjugate labeled with the first TMT to produce a first mixture comprising one or more unlabeled peptides and one or more peptides labeled with the first TMT; (iii) contacting the first mixture with a second tandem mass tag (TMT), thereby obtaining a second mixture comprising one or more peptides labeled with at least one of the first TMT and the second TMT, and optionally one or more unlabeled peptides, wherein the first TMT and the second TMT do not have the same reporter ion mass; (iv) subjecting the second mixture to liquid chromatography (LC) to produce an LC eluate; (v) subjecting the eluate to tandem mass spectrometry to obtain mass spectra of peptides containing reporter ions of at least one of the first TMT and the second TMT; (vi) detecting the mass-to-charge ratio (m / z) associated with said at least one reporter ion, thereby analyzing said conjugate in said sample. [Invention 2] 1. A method for analyzing a conjugate comprising a drug covalently attached to a polypeptide, comprising: (i) contacting a sample containing the conjugate with a first tandem mass tag (TMT), thereby labeling the polypeptide of the conjugate with the first TMT; (ii) digesting the polypeptide of the conjugate labeled with the first TMT to produce a first mixture comprising one or more unlabeled peptides and one or more peptides labeled with the first TMT; (iii) contacting the first mixture with a second tandem mass tag (TMT), thereby obtaining a second mixture comprising one or more peptides labeled with at least one of the first TMT and the second TMT, and optionally one or more unlabeled peptides; (iv) contacting a control sample comprising the polypeptide that is not covalently bound to the drug with a third TMT, thereby labeling the polypeptide that is not covalently bound to the drug with the third TMT; (v) digesting the polypeptide that is not covalently attached to the third TMT-labeled drug to produce a third mixture comprising one or more unlabeled peptides and one or more peptides labeled with the third TMT; (vi) contacting the third mixture with a fourth tandem mass tag (TMT), thereby obtaining a fourth mixture comprising one or more peptides labeled with at least one of the third TMT and the fourth TMT, and optionally one or more unlabeled peptides; (vii) combining the second mixture with the fourth mixture and subjecting the combination to liquid chromatography (LC) to generate an LC eluate; (viii) subjecting the eluate to tandem mass spectrometry to obtain mass spectra of peptides containing reporter ions of at least one of the first TMT, the second TMT, the third TMT, and the fourth TMT; (ix) detecting a mass-to-charge ratio associated with the at least one reporter ion, thereby analyzing the conjugate in the sample; wherein none of the first TMT, the second TMT, the third TMT, and the fourth TMT have the same reporter ion mass; the first TMT and the third TMT are selected from a first isobaric set of TMTs; the second TMT and the fourth TMT are selected from a second isobaric set of TMTs; the first isobaric set of TMTs are reactive to unconjugated amino acid residues capable of forming a covalent bond with the drug; and the second isobaric set of TMTs are reactive to lysine or a free amine at the N-terminus of a peptide. [Invention 3] 1. A method for determining the occupancy of a conjugation site in a conjugate, comprising: 1) using the method of invention 2 to obtain mass spectra for a peptide labeled with both the first TMT and the second TMT and a peptide labeled with both the third TMT and the fourth TMT, wherein the mass spectra comprise a reporter ion of the first TMT, a reporter ion of the third TMT, a reporter ion of the second TMT, and a reporter ion of the fourth TMT; 2) detecting a mass-to-charge ratio (m / z) associated with the reporter ion in the mass spectrum; and 3) determining the occupancy of the conjugation sites in the conjugate based on the intensity of the reporter ion of the first TMT and the intensity of the reporter ion of the third TMT, or the intensity of the reporter ion of the second TMT and the intensity of the reporter ion of the fourth TMT in the mass spectrum, preferably according to the following formula: (intensity of the reporter ion of the third TMT−intensity of the reporter ion of the first TMT) / intensity of the reporter ion of the third TMT, or and determining an intensity of the reporter ion of the fourth TMT, wherein the intensity of the reporter ion of the fourth TMT is determined by (intensity of the reporter ion of the fourth TMT - intensity of the reporter ion of the second TMT) / intensity of the reporter ion of the fourth TMT. [Invention 4] 4. The method of claim 3, wherein the occupancy of the conjugation sites in the conjugate is determined at various time points and additional TMT is used to label the polypeptide at different time points. [Invention 5] 1. A method for normalizing a sample containing a conjugate to a control sample, comprising: 1) obtaining mass spectra for the second TMT-only labeled peptide and the fourth TMT-only labeled peptide using the method of invention 2, wherein the mass spectra contain reporter ions of the second TMT and the fourth TMT, but do not contain reporter ions of the first TMT or the third TMT; 2) detecting the mass-to-charge ratio (m / z) associated with the reporter ion; 3) normalizing the sample to the control sample by the ratio of the intensity of the reporter ion of the second TMT to the intensity of the reporter ion of the fourth TMT. [Invention 6] 1. A method for localizing a drug conjugation site in a conjugate, comprising: 1) obtaining a mass spectrum of a peptide labeled with only the second TMT using the method of invention 2, wherein the mass spectrum contains only reporter ions of the second TMT but does not contain reporter ions of the first, third, or fourth TMTs; 2) triggering a second tandem mass spectrometry analysis on the peptide labeled with only the reporter ion of the second TMT, thereby localizing the drug conjugation site. [Invention 7] 7. The method of claim 6, wherein the peptide is fully conjugated to the drug. [Invention 8] 1. A method for localizing a drug conjugation site in a conjugate, comprising: 1) obtaining a mass spectrum of a peptide labeled with only the first and second TMTs using the method of invention 2, wherein the mass spectrum contains only reporter ions of the first and second TMTs but does not contain reporter ions of the third or fourth TMTs; 2) triggering a second tandem mass spectrometry analysis on the peptide labeled with only the first and second TMTs, thereby localizing the drug conjugation site. [Invention 9] 9. The method of claim 8, wherein the peptide is not fully conjugated to the drug. [Invention 10] 10. The method according to any one of Inventions 1 to 9, wherein the tandem mass spectrometry is high-energy collision-induced dissociation tandem mass spectrometry (HCD-MS2). [Invention 11] 11. The method according to any one of Inventions 6 to 10, wherein the second tandem mass spectrometry is electron transfer dissociation tandem mass spectrometry (ETD-MS2) or electron capture dissociation tandem mass spectrometry (ECD-MS2). [Invention 12] 12. The method according to any one of claims 6 to 11, wherein a higher trigger intensity threshold and / or a narrower isolation window is used to improve triggering of said second tandem mass spectrometry method. [Invention 13] 13. The method according to any one of claims 1 to 12, wherein synchronous precursor selection using tribrid technology is applied to said tandem mass spectrometry to improve the specificity and accuracy of said detection and quantification. [Invention 14] 14. The method of any one of claims 1 to 13, wherein the first TMT and the third TMT each comprise a mass reporter, a mass normalizer, and a cysteine-reactive group covalently bonded to each other, and the second TMT and the fourth TMT each comprise a mass reporter, a mass normalizer, and an amine-reactive group covalently bonded to each other. [Invention 15] 15. The method of claim 14, wherein each of the first TMT and the third TMT is selected from an isobaric set of IodoTMTsixplex. [Invention 16] 16. The method of claim 14 or 15, wherein each of the second TMT and the fourth TMT is selected from an isobaric set of TMT6plex, TMT10plex, or TMT pro16plex. [Invention 17] 17. The method according to any one of Inventions 1 to 16, wherein the conjugate is an antibody-drug conjugate (ADC). [Invention 18] 18. The method according to any one of claims 1 to 17, wherein a multiplex of samples containing one or more conjugates is analyzed. [Invention 19] 19. The method according to any one of Inventions 1 to 18, wherein said conjugate or said ADC comprises one or more drugs conjugated to cysteine ​​residues of said polypeptide or said antibody. [Invention 20] 14. The method according to any one of Inventions 1 to 13, wherein said conjugate or said ADC comprises one or more drugs conjugated to lysine residues of said polypeptide or said antibody. [Invention 21] 21. The method of claim 20, wherein the first TMT, second TMT, third TMT, and fourth TMT each comprise a mass reporter, a mass normalizer, and an amine-reactive group covalently bonded to one another. [Invention 22] 22. The method of claim 21, wherein each of the first TMT, the second TMT, the third TMT, and the fourth TMT is selected from a TMT6plex, a TMT10plex, or a TMT pro16plex. [Invention 23] 23. The method according to any one of claims 1 to 22, wherein the drug conjugation site in the conjugate is characterized by a mass spectrometry barcode comprising (2n+2) reporter ions, and the conjugate is normalized by a mass spectrometry barcode comprising n+1 reporter ions, where n is the number of samples analyzed by the method. [Invention 24] A system for carrying out any of the methods described in any one of inventions 1 to 23. [Invention 25] A composition comprising a mixture of peptides labeled with at least one of a first TMT and a second TMT, and optionally one or more unlabeled peptides, wherein the first TMT and the second TMT do not have the same reporter ion mass, and the mixture of peptides comprises at least one peptide conjugated to a drug and labeled with at least one of the first TMT and the second TMT.

Claims

1. 1. A method for analyzing a conjugate comprising a drug covalently attached to a polypeptide, comprising: (i) contacting a sample containing the conjugate with a first tandem mass tag (TMT), thereby labeling the polypeptide of the conjugate with the first TMT; (ii) digesting the polypeptide of the conjugate labeled with the first TMT to produce a first mixture comprising one or more unlabeled peptides and one or more peptides labeled with the first TMT; (iii) contacting the first mixture with a second tandem mass tag (TMT), thereby obtaining a second mixture comprising one or more peptides labeled with at least one of the first TMT and the second TMT, wherein the first TMT and the second TMT do not have the same reporter ion mass; (iv) subjecting the second mixture to liquid chromatography (LC) to produce an LC eluate; (v) subjecting the eluate to tandem mass spectrometry to obtain a mass spectrum of peptides containing reporter ions of at least one of the first TMT and the second TMT; (vi) detecting the mass-to-charge ratio (m / z) associated with said at least one reporter ion, thereby analyzing said conjugate in said sample.

2. The method of claim 1 , wherein the second mixture comprises one or more unlabeled peptides.

3. 1. A method for analyzing a conjugate comprising a drug covalently attached to a polypeptide, comprising: (i) contacting a sample containing the conjugate with a first tandem mass tag (TMT), thereby labeling the polypeptide of the conjugate with the first TMT; (ii) digesting the polypeptide of the conjugate labeled with the first TMT to produce a first mixture comprising one or more unlabeled peptides and one or more peptides labeled with the first TMT; (iii) contacting the first mixture with a second tandem mass tag (TMT) to thereby obtain a second mixture comprising one or more peptides labeled with at least one of the first TMT and the second TMT; (iv) contacting a control sample containing the polypeptide that is not covalently bound to the drug with a third TMT, thereby labeling the polypeptide that is not covalently bound to the drug with the third TMT; (v) digesting the polypeptide that is not covalently attached to the third TMT-labeled drug to produce a third mixture comprising one or more unlabeled peptides and one or more peptides labeled with the third TMT; (vi) contacting the third mixture with a fourth tandem mass tag (TMT) to thereby obtain a fourth mixture comprising one or more peptides labeled with at least one of the third TMT and the fourth TMT; (vii) combining the second mixture with the fourth mixture and subjecting the combination to liquid chromatography (LC) to generate an LC eluate; (viii) subjecting the eluate to tandem mass spectrometry to obtain a mass spectrum of peptides containing reporter ions of at least one of the first TMT, the second TMT, the third TMT, and the fourth TMT; (ix) detecting a mass-to-charge ratio associated with the at least one reporter ion, thereby analyzing the conjugate in the sample; wherein none of the first TMT, the second TMT, the third TMT, and the fourth TMT have the same reporter ion mass, the first TMT and the third TMT are selected from a first isobaric set of TMTs, the second TMT and the fourth TMT are selected from a second isobaric set of TMTs, the first isobaric set of TMTs are reactive to unconjugated amino acid residues capable of forming a covalent bond with the drug, and the second isobaric set of TMTs are reactive to lysine or a free amine at the N-terminus of a peptide.

4. The method of claim 3 , wherein the second mixture, the fourth mixture, or the second mixture and the fourth mixture comprises one or more unlabeled peptides.

5. 1. A method for determining the occupancy of a conjugation site in a conjugate, comprising: 1) obtaining mass spectra for a peptide labeled with both the first TMT and the second TMT and a peptide labeled with both the third TMT and the fourth TMT using the method of claim 3, wherein the mass spectra include a reporter ion of the first TMT, a reporter ion of the third TMT, a reporter ion of the second TMT, and a reporter ion of the fourth TMT; 2) detecting a mass-to-charge ratio (m / z) associated with the reporter ion in the mass spectrum; and 3) determining the occupancy of the conjugation site in the conjugate based on the intensity of the reporter ion of the first TMT and the intensity of the reporter ion of the third TMT, or the intensity of the reporter ion of the second TMT and the intensity of the reporter ion of the fourth TMT in the mass spectrum.

6. The occupancy is expressed by the following formula: (intensity of the reporter ion of the third TMT−intensity of the reporter ion of the first TMT) / intensity of the reporter ion of the third TMT; or (intensity of the reporter ion of the fourth TMT−intensity of the reporter ion of the second TMT) / intensity of the reporter ion of the fourth TMT The method of claim 5 , wherein the value is determined by

7. 6. The method of claim 5, wherein the occupancy of the conjugation sites in the conjugate is determined at various time points and additional TMT is used to label the polypeptide at different time points.

8. 1. A method for normalizing a sample containing a conjugate to a control sample, comprising: 1) obtaining mass spectra for the second TMT-only labeled peptide and the fourth TMT-only labeled peptide using the method of claim 3, wherein the mass spectra include a reporter ion for the second TMT and a reporter ion for the fourth TMT, but do not include a reporter ion for the first TMT or a third TMT; 2) detecting the mass-to-charge ratio (m / z) associated with the reporter ion; 3) normalizing the sample to the control sample by the ratio of the intensity of the reporter ion of the second TMT to the intensity of the reporter ion of the fourth TMT.

9. 1. A method for localizing a drug conjugation site in a conjugate, comprising: 1) obtaining a mass spectrum of a peptide labeled with only the second TMT using the method of claim 3, wherein the mass spectrum contains only the reporter ion of the second TMT, but does not contain the reporter ions of the first, third, or fourth TMTs; 2) triggering a second tandem mass spectrometry analysis on the peptide labeled with only the reporter ion of the second TMT, thereby localizing the drug conjugation site.

10. 10. The method of claim 9, wherein the peptide is fully conjugated to the drug.

11. 1. A method for localizing a drug conjugation site in a conjugate, comprising: 1) obtaining a mass spectrum of a peptide labeled with only the first and second TMTs using the method of claim 3, wherein the mass spectrum contains only reporter ions of the first and second TMTs, but does not contain reporter ions of the third or fourth TMTs; 2) triggering a second tandem mass spectrometry analysis on the peptide labeled with the first and second TMTs only, thereby localizing the drug conjugation site.

12. 12. The method of claim 11, wherein the peptide is not fully conjugated to the drug.

13. The method according to any one of claims 1 to 12, wherein the tandem mass spectrometry is high-energy collision-induced dissociation tandem mass spectrometry (HCD-MS2).

14. The method according to any one of claims 9 to 13, wherein the second tandem mass spectrometry is electron transfer dissociation tandem mass spectrometry (ETD-MS2) or electron capture dissociation tandem mass spectrometry (ECD-MS2).

15. The method of any one of claims 9 to 14, wherein a higher trigger intensity threshold and / or a narrower isolation window is used to improve triggering of the second tandem mass spectrometry method.

16. The method of any one of claims 1 to 15, wherein synchronous precursor selection using tribrid technology is applied to the tandem mass spectrometry method to improve the specificity and accuracy of the detection and quantification.

17. 17. The method of any one of claims 1 to 16, wherein the first TMT and the third TMT each comprise a mass reporter, a mass normalizer, and a cysteine-reactive group covalently bonded to one another, and the second TMT and the fourth TMT each comprise a mass reporter, a mass normalizer, and an amine-reactive group covalently bonded to one another.

18. 18. The method of claim 17, wherein each of the first TMT and the third TMT is selected from an isobaric set of IodoTMTsixplex.

19. 19. The method of claim 17 or 18, wherein each of the second TMT and the fourth TMT is selected from an isobaric set of TMT6plex, TMT10plex, or TMT pro16plex.

20. The method of any one of claims 1 to 19, wherein the conjugate is an antibody drug conjugate (ADC).

21. The method of any one of claims 1 to 20, wherein a multiplex of samples containing one or more conjugates is analyzed.

22. 22. The method of any one of claims 1 to 21, wherein the conjugate comprises one or more drugs coupled to a cysteine ​​residue of the polypeptide.

23. 17. The method of any one of claims 1 to 16, wherein the conjugate comprises one or more drugs coupled to a lysine residue of the polypeptide.

24. 24. The method of claim 23, wherein the first TMT, second TMT, third TMT, and fourth TMT each comprise a mass reporter, a mass normalizer, and an amine-reactive group covalently bonded to one another.

25. 25. The method of claim 24, wherein each of the first TMT, second TMT, third TMT, and fourth TMT is selected from a TMT6plex, a TMT10plex, or a TMT pro16plex.

26. 26. The method of any one of claims 1 to 25, wherein the drug conjugation moieties in the conjugates are characterized by mass spectrometry barcodes comprising (2n+2) reporter ions, and the conjugates are normalized by mass spectrometry barcodes comprising n+1 reporter ions, where n is the number of samples analyzed by the method.

27. A system for carrying out any of the methods according to any one of claims 1 to 26.

28. A composition comprising a mixture of peptides labeled with at least one of a first TMT and a second TMT, wherein the first TMT and the second TMT do not have the same reporter ion mass, and the mixture of peptides comprises at least one peptide conjugated to a drug and labeled with at least one of the first TMT and the second TMT.

29. 30. The composition of claim 28, further comprising one or more unlabeled peptides.

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