Cosmetic composition using processed beer lees and its manufacturing method

Abstract

The method according to an embodiment of the present invention includes the steps of sterilizing, drying and pulverizing brewers' spent material; classifying the pulverized brewers' spent material according to particle size conditions and carrying out post-processing by a post-processing method to prepare brewers' spent material scrub or brewers' spent material capsules in advance; separately measuring and preparing an aqueous phase and an oil phase of a cosmetic raw material for manufacturing a cosmetic composition containing the brewers' spent material scrub or brewers' spent material capsules; heating the aqueous phase and the oil phase in a hot water bath, and then stirring the aqueous phase and adding the oil phase to perform an emulsification process; cooling the emulsified composition; adding the previously prepared brewers' spent material scrub or brewers' spent material capsules and stirring; and vacuum degassing the stirred composition.

Description

[Technical Field]

[0001] The present invention relates to a cosmetic composition and a method for producing the same, and more specifically, the present invention relates to a cosmetic composition using a processed product of brewers' spent grains and a method for producing the same. [Background technology]

[0002] Modern humans are prone to various skin problems caused by internal and external factors such as ultraviolet rays and stress, which accelerate skin aging phenomena such as age spots, freckles, and skin pigmentation (1. Voegeli, R. 1996. Elastase and tryptase determination on the human skin surface. Cosmetic & Toiletries. 111, 51-58.). Skin pigmentation is known to regulate the initial reactions in melanin biosynthesis, including the conversion of L-tyrosine to DOPA (3,4-dihydroxyphenylalanine) and DOPA to DOPAquinone, via tyrosinase enzymes and DHICA oxidase (TRP-1) (2. Aroca, P., et al. 1993. Melanin biosynthesis patterns of following hormonal stimulation. J. Biol. Chem. 268, 25650-25655.). Based on this, active research is being conducted to discover natural products that may inhibit the activity of the enzyme tyrosinase and thus suppress melanin biosynthesis. As a result, research utilizing natural products is actively being conducted, such as the use of Houttuynia cordata extracts to inhibit tyrosinase gene expression (3. Chin, JE, et al. 2005. Effects of Houttuynia cordata extracts on tyrosinase gene expression. J. Korean Soc Food Sci Nutr 34, 1284-1288.). Other currently known antioxidant and whitening ingredients include arbutin, kojic acid, and ascorbic acid, as well as plant extracts such as kozo (paper mulberry) and licorice.

[0003] Brewer's spent grain (BSG) accounts for approximately 80-90% of the by-products generated during the beer brewing process. BSG is a by-product of the germination and mashing of two-row barley, the main ingredient in beer production, and the separation of the mash. It is obtained as the residue left over from the process of adding malt to barley grains to obtain wort, which is then processed to produce beer. BSG is essentially made up of the husk, pericarp, and seed coat layers of barley, and is rich in minerals and vitamins, with particularly high dietary fiber and protein content.

[0004] Brewers' grains are highly nutritious, containing large amounts of dietary fiber and essential amino acids despite their low price, making them highly valuable not only as food but also as livestock feed. In fact, reports have shown increased milk production in livestock fed diets containing brewers' grains, and fish fed diets containing brewers' grains have shown higher weight gain compared to fish fed diets containing rice bran alone. Brewers' grains contain high amounts of lactogenic growth hormones, particularly prolactin, and have long been used to promote the growth, weight gain, and high-quality milk production of sheep and cattle. Furthermore, research into their commercial value has been conducted in the baking industry, where brewers' grains are used to make bread, flakes, and biscuits. In actual food applications, adding brewers' grains to bread and cookies has shown improved flavor, and clinical trials have shown effects such as reducing lipids and cholesterol and alleviating constipation. As such, brewers' grains are a food ingredient that provides high dietary fiber and have ample potential for expansion into industries other than food.

[0005] However, up until now, the beer grains have only been used as livestock feed or as a food ingredient, and the beer grains that cannot be used for this purpose are discarded. Summary of the Invention [Problem to be solved by the invention]

[0006] The present invention has been made to solve these problems, and its purpose is to provide a cosmetic composition using processed beer grains, which can utilize the entire beer grains economically and environmentally by processing and upcycling the rich fiber and active ingredients of beer grains so that they are suitable as cosmetic ingredients, and a method for producing the same. [Means for solving the problem]

[0007] To achieve the above object, a method according to an embodiment of the present invention comprises: The method includes the steps of sterilizing brewers' spent material, drying the sterilized brewers' spent material, pulverizing the dried brewers' spent material, classifying the pulverized brewers' spent material according to particle size conditions, determining a post-processing method corresponding to the classified particle size conditions, and producing brewers' spent material for producing a category-specific cosmetic composition according to the determined post-processing method.

[0008] Furthermore, the particle size conditions correspond to grinding size conditions, and the grinding size conditions can include at least one of a 50 mesh or less condition, a 50 mesh condition, a 1000 mesh condition, and a 3000 mesh condition.

[0009] In addition, when the pulverization size condition is fine powder, the post-processing method includes pulverizing the pulverized brewers' spent material, mixing the pulverized brewers' spent material with capsule seeds, granulating the pulverized brewers' spent material, and then coating and encapsulating the pulverized brewers' spent material.

[0010] Furthermore, when the grinding size condition is 3000 mesh, the category of the cosmetic composition is a body scrub; when the grinding size condition is 1000 mesh, the category of the cosmetic composition is a body cleanser; and when the grinding size condition is 50 mesh, the category of the cosmetic composition is a face scrub.

[0011] The post-processing method may also include a process of obtaining a brewer's spent grain extract by performing hot water extraction and filtration from the pulverized brewer's spent grain raw material.

[0012] The cosmetic composition contains the processed brewers' grains as an active ingredient, and is thus manufactured into a brewers' grain extract-containing cosmetic having antibacterial, whitening, antioxidant and melanin production inhibitory effects.

[0013] Furthermore, the processed brewer's spent grains obtained by the above-described method can be produced into a cosmetic composition containing the processed brewer's spent grains as an active ingredient. [Effects of the Invention]

[0014] According to an embodiment of the present invention, the fiber and active ingredients contained in brewer's grains in abundance can be utilized to the fullest extent in the cosmetics industry, so that a cosmetic raw material having a whitening effect and a cosmetic composition containing the same can be processed.

[0015] Therefore, according to an embodiment of the present invention, brewers' spent grains can be utilized as a cosmetic composition in addition to their existing uses, and 100% of the spent grains can be utilized without generating additional waste, thereby providing an economical and environmentally friendly production process for whitening cosmetics. [Brief explanation of the drawings]

[0016] [Figure 1] 1 is a flowchart illustrating a processed beer grain product, a cosmetic composition using the same, and a method for producing the same according to an embodiment of the present invention. [Figure 2]1 is an illustration of a processed beer spent product and a cosmetic composition using the same according to an embodiment of the present invention. [Figure 3] 1 is an illustration of a processed beer spent product and a cosmetic composition using the same according to an embodiment of the present invention. [Figure 4] 1 is a diagram illustrating an encapsulation process of processed brewers' spent grains according to an embodiment of the present invention. FIG. [Figure 5] FIG. 1 is an illustration of an encapsulated beer spent product. [Figure 6] 2 is a flowchart for more specifically illustrating a method for producing a scrub and capsule-containing cosmetic composition made from processed brewers' spent grains according to an embodiment of the present invention. [Figure 7] 1 is a flowchart for more specifically illustrating a method for producing a cosmetic composition containing an extract of processed brewers' spent grains according to an embodiment of the present invention. [Figure 8] 1 is a diagram illustrating the effects of a cosmetic product containing brewer's spent grain extract according to an embodiment of the present invention. [Figure 9] 1 is a diagram illustrating the effects of a cosmetic product containing brewer's spent grain extract according to an embodiment of the present invention. [Figure 10] 1 is experimental data showing a comparison of extraction yields according to drying processing methods of brewer's spent grains according to an embodiment of the present invention. [Figure 11] 1 shows comparative data of efficacy experiments on the extract concentration during hot water extraction of a brewers' spent grain extract according to one embodiment of the present invention and the processed concentration of the extracted brewers' spent grain hot water extract processed product. [Figure 12] 1 shows an analysis chart of the influence of different processing concentrations of brewers' grains processed products using brewers' grain extracts according to an embodiment of the present invention on hair growth / hair development-related growth factors in human hair dermal papilla cells. DETAILED DESCRIPTION OF THE INVENTION

[0017] Hereinafter, a processed beer lees product, a cosmetic composition using the same, and a method for producing the same according to an embodiment of the present invention will be described in more detail with reference to the drawings.

[0018] FIG. 1 is a flowchart illustrating a processed brewer's spent grain product, a cosmetic composition using the same, and a method for producing the same according to an embodiment of the present invention.

[0019] Brewers' spent grains contain all solids separated from wort by filtration, including the remains of barley malt and adjuncts. When corn grit is used as the adjunct, the brewers' spent grains are primarily composed of the husk and husk portions of barley and the non-starch portions of corn. Brewers' spent grains are lignocellulosic materials that typically contain lipids, lignin, protein, cellulose, hemicellulose, and small amounts of ash. In describing and claiming this invention, the term "brewers' spent grains" (BSG) will be used in accordance with the definition hereinabove.

[0020] Barley is also the primary raw material used in the production of brewer's grains, although other grains such as corn and rice are typically used along with two-row barley. During the brewing process, the starchy endosperm of these grains is enzymatically degraded, liberating fermentable (maltose, maltotriose, and a small percentage of glucose) and non-fermentable carbohydrates (dextrins), proteins, polypeptides, and amino acids. The resulting medium, which is then fermented into beer by the action of yeast, is known as wort. The insoluble grain components (mainly containing grain husks) are brewer's grains (BSG). In traditional brewing using a lauter tun, the BSG components play an important role, forming the layer through which the mash is filtered to produce wort. Therefore, initial milling of the malt must ensure that the grain husks remain intact to form a suitable filter. Today, many small or artisan breweries still use this mash filtration method, but many larger breweries use mash filters that rely less on the filtering capabilities of the BSG, so the malt can be milled more extensively.

[0021] FIG. 1 is a diagram illustrating a process for processing a cosmetic composition using such brewer's spent grains, and FIGS. 2 and 3 are diagrams illustrating examples of a brewer's spent grain processed product and a cosmetic composition using the same according to an embodiment of the present invention.

[0022] First, referring to FIG. 1, beer grains are subjected to a sterilization process (S101).

[0023] The sterilization environment during the sterilization process is preferably 121°C for 0.5 to 1.0 hour.

[0024] In addition, the sterilized brewer's grains are subjected to a drying treatment (S103).

[0025] The drying environment during the drying process is preferably 50 to 70°C for 2 to 6 hours, but various drying methods can be applied.

[0026] More specifically, according to the first embodiment, the drying environment can be exemplified by a method in which the sterilized brewer's spent grains are dried at 45° C. for 24 hours.

[0027] According to the second embodiment, the drying environment may be, for example, freeze-drying for 22 hours followed by drying at 75° C. for 2 hours.

[0028] According to the third embodiment, the drying environment can be exemplified by a method of drying with hot air, for example.

[0029] The dried brewer's grains are then crushed (S105).

[0030] Here, the brewer's spent grains to be pulverized may have sizes corresponding to meshes according to various particle size conditions.

[0031] As a result, the crushed beer grains are classified according to the crushed grain size (S107).

[0032] More specifically, referring to Fig. 2, the particle size conditions of brewers' spent grains are preferably classified into fine powder, 50 mesh, 1000 mesh, 3000 mesh, etc. Examples of the fine powder conditions include 173 µm or less, 50 mesh corresponding to 173 to 279 µm, 1000 mesh corresponding to 279 to 864 µm, and 3000 mesh corresponding to 864 to 1520 µm.

[0033] Then, the post-processing method of the brewers' spent grains is determined based on the particle size classification (S109), and cosmetic compositions of each category corresponding to the determined processing method are produced (S111).

[0034] More specifically, referring to FIG. 3, the meshes for each particle size condition can be used to manufacture different categories of cosmetics through different processing methods.

[0035] For example, as shown in Figure 3, 3000 mesh can be manufactured as a body scrub, 1000 mesh can be manufactured as a body cleanser, and 50 mesh can be manufactured as a face scrub, and the fine powder can be processed into beer grain capsules and manufactured as a base dosage form to be mixed with various other cosmetic ingredients.

[0036] Furthermore, as confirmed by various experimental results described below, the efficacy of processed brewers' spent grains varies depending on the processing concentration of the processed brewers' spent grains. Thus, the cosmetic composition according to the present invention can optimize its effects according to the purpose, such as whitening, antioxidation, hair growth, and hair care, by adjusting the processing concentration of the processed brewers' spent grains according to the particle size classification.

[0037] FIG. 4 is a diagram illustrating the encapsulation process of the processed product of brewers' spent grains according to an embodiment of the present invention, and FIG. 5 is an example of the encapsulated processed product of brewers' spent grains.

[0038] Referring to FIG. 4, first, the sterilization, drying and crushing processes of the brewers' grains are carried out in the above-mentioned S101 and S103, and then, when a coarse powder is obtained, it is crushed into fine powder for encapsulation (S201).

[0039] The micronization process results in a powder having a size of 173 μm or less, which is smaller than 50 mesh.

[0040] Then, capsule seeds for encapsulation are produced (S202). The capsule seeds may contain capsule polymers for cosmetic production, which may contain beer grain fine powder.

[0041] The capsule seeds are then mixed with brewer's spent grains and granulated (S203).

[0042] The granulated beer spent grains are then encapsulated by a coating process (S205).

[0043] 5 shows encapsulated brewers' spent grains granules, which can be used as a base formulation for the aforementioned cosmetics using brewers' spent grains. Examples of base formulations include skin lotions, skin care products, lotions, creams, massage creams, essences, and mask packs, and by adding the encapsulated brewers' spent grains granules, basic cosmetics using processed brewers' spent grains can be produced.

[0044] FIG. 6 is a flowchart for more specifically explaining the method for producing a scrub and capsule-containing cosmetic composition made from processed brewers' spent grains according to an embodiment of the present invention.

[0045] Referring to FIG. 6, first, the aqueous phase and oil phase of the cosmetic raw materials for producing the scrub and capsule-containing cosmetic composition are separately measured and prepared (S301).

[0046] Thereafter, the aqueous phase and the oil phase are each heated in a hot water bath (S303).

[0047] Here, the water bath is preferably heated to about 90°C.

[0048] Further, an emulsification treatment is carried out by stirring the aqueous phase and adding the oil phase (S305).

[0049] Thereafter, the emulsified composition is subjected to a cooling treatment (S307).

[0050] Here, the cooling treatment is preferably carried out at about 50° C. or below.

[0051] Furthermore, beer spent scrub or beer spent capsules prepared in advance by the method shown in FIG. 1 or FIG. 4 are added to the cooled composition and stirred (S309).

[0052] Thereafter, the stirred composition is subjected to a vacuum degassing treatment (S311).

[0053] The scrub and capsule-containing cosmetic composition of processed brewer's spent grains produced by the above-mentioned method for producing a scrub and capsule-containing cosmetic composition can be produced as a variety of products, such as a body scrub, a face scrub, a cleanser, a scalp scaling product, a body wash, a facial wash, a shampoo, etc.

[0054] FIG. 7 is a flowchart for more specifically illustrating the method for producing a cosmetic composition containing an extract of processed brewers' spent grains according to an embodiment of the present invention.

[0055] Referring to FIG. 7, steps S101 to S103 in FIG. 1 are performed, and dried beer grains are crushed and mashed (S401).

[0056] Here, the pulverized beer spent grains may be exemplified as those having a mesh size of 50, which indicates that the grains are pulverized to a size of approximately 173 to 279 μm.

[0057] Then, the mashed beer grain powder is extracted with hot water (S403).

[0058] More specifically, in the hot water extraction, the mashed brewer's spent grains may be dried in various ways, but it is preferable to mix them in a predetermined weight ratio, weight-to-volume ratio, or concentration ratio depending on the state and then perform hot water extraction.

[0059] Preferably, 10 g of mashed brewers' spent grains are mixed with 200 mL of distilled water (DW) to prepare a mixture for hot water extraction at a weight-to-volume ratio of 5%, and the mixture for hot water extraction is extracted in a shaking water bath at 70°C for 3 hours at 50 rpm, thereby maximizing the efficacy and effects of the brewers' spent grains extract described below.

[0060] Thereafter, the hot water extracted brewer's spent grain extract is filtered (S405).

[0061] The hot water extracted brewer's spent grain extract obtained as described above can be filtered through filter paper made of quantitative filter paper. Preferably, filter paper with a pore size of 20 μm can be used.

[0062] The filtered brewer's spent grain extract can also be processed by freeze-drying for 48 hours to produce a mixture that can be stirred into other cosmetic composition formulations.

[0063] The filtered brewer's spent extract is then mixed with other cosmetic composition formulations by stirring to produce a brewer's spent extract-containing cosmetic product (S407).

[0064] 8 and 9 are diagrams illustrating the effects of the beer spent extract-containing cosmetic product according to an embodiment of the present invention.

[0065] First, FIG. 8(A) shows a comparison of the total polyphenol content of processed brewers' spent grains produced according to the embodiment of the present invention, and it can be seen that the content is approximately 25 to 35 mgGAE / g depending on the concentration of brewers' spent grains.

[0066] Furthermore, Figure 8(B) shows a comparison of the total flavonoid content of processed brewers' spent grains produced according to the embodiment of the present invention, and it can be seen that the content is approximately 150 to 200 mgQE / g depending on the concentration of brewers' spent grains.

[0067] Flavonoids and polyphenols are known to have excellent antibacterial and whitening effects, and it has been confirmed that the brewer's grain extract-containing cosmetics using the brewer's grain processed product according to an embodiment of the present invention have sufficient antibacterial and whitening effects.

[0068] 8(C) shows a comparison of the antioxidant activity of the brewer's spent processed products prepared according to the embodiment of the present invention using a DPPH assay, which confirms that the antioxidant activity increases depending on the concentration of brewer's spent. Therefore, it can be confirmed that the brewer's spent extract-containing cosmetics using the brewer's spent processed products according to the embodiment of the present invention have sufficient antioxidant activity depending on the concentration.

[0069] FIG. 9 shows the results of testing the melanin production inhibitory effect of the processed brewer's spent grains produced according to an embodiment of the present invention.

[0070] As shown in Figure 9, it can be confirmed that the melanin increased by α-MSH in B16F10 cells, which are melanocytes, is reduced in a concentration-dependent manner by the processed hot water extract of brewers' grains shown in the lower panel.

[0071] In particular, it can be confirmed that even at a low processing concentration of 200 μg / mL, when the brewer's spent grain extract was processed, melanin production was inhibited at a level equivalent to that of the negative control (NC).

[0072] This confirms that the cosmetics containing brewer's grain extract using the brewer's grain processed product according to the examples of the present invention have an excellent effect of inhibiting melanin production.

[0073] Meanwhile, FIG. 10 shows experimental data comparing extraction yields according to drying processing methods of brewer's spent grains according to an embodiment of the present invention.

[0074] As mentioned above, in the case of brewer's spent grains, the drying processing conditions are different from each other, so the experimental data shows the extraction yield and effect measured according to the first, second and third embodiments.

[0075] More specifically, the drying environment in the first embodiment involves drying the sterilized brewer's spent grains at 45°C for 24 hours, the drying environment in the second embodiment involves freeze-drying for 22 hours followed by drying at 75°C for 2 hours, and the drying environment in the third embodiment involves hot air drying.

[0076] 200 mL of distilled water (DW) was then added to 10 g of brewers' spent grains dried and mashed using each of the three drying methods to prepare a mixture for hot water extraction at a weight-to-volume ratio of 5%. The mixture for hot water extraction was then extracted in a shaking water bath at 70°C for 3 hours at 50 rpm. The hot water extracted brewers' spent grains extract was filtered through filter paper made from quantitative filter paper, with a pore size of 20 μm.

[0077] As shown in Figure 10, the yield of brewer's spent grain extract in a drying environment was significantly higher in the third embodiment, which involved hot air drying, and the polyphenol content also increased accordingly. However, the antioxidant activity due to DPPH radical scavenging activity was relatively higher in the first and second embodiments.

[0078] Therefore, in the method for producing a brewers' spent grain extract according to an embodiment of the present invention, the drying environment in the production step can be varied by selecting one of the following methods: a method in which the drying is performed at a first temperature of 40°C or higher for a certain period of time as in the first embodiment; a method in which freeze-drying is performed for a first hour and then drying at a second temperature of 70°C or higher for a second period of time that is shorter than the first period as in the second embodiment; and a method in which hot air drying is performed for a certain period of time as in the third embodiment. By adjusting the selective drying method, it is possible to appropriately adjust the target polyphenol content and target antioxidant capacity according to the characteristics of the cosmetic composition, and therefore it can be seen that the yield of the brewers' spent grain extract according to the target efficacy can be optimized by varying the drying method.

[0079] Meanwhile, FIG. 11 shows comparative data of efficacy experiments on the extract concentration during hot water extraction of brewers' spent grain extract according to an embodiment of the present invention and the processed concentration of the extracted brewers' spent grain hot water extract processed product.

[0080] 11, the extraction concentrations of the brewer's spent grains hot water extract were set to 5%, 15%, and 30%, and the weight-to-volume ratios of the processing concentrations for each extraction concentration were set to 25, 50, 100, 200, 400, and 800 μg / mL. Since distilled water (DW) was used as the solvent, assuming that the specific gravity of distilled water is generally 1, the processing concentrations can be expressed as 25, 50, 100, 200, 400, and 800 ppm (parts per million) based on the total weight of the composition.

[0081] First, Figures 11(A) and 11(B) are for cell safety testing, and show MTT assay charts for cell viability analysis of HaCaT (Human, Adult, Low Calcium, High Temperature) cells to measure the effects on the human body at each processing concentration of the brewer's spent grain hot water extract at a concentration of 5%, and B16F10 cells to measure melanin.

[0082] As shown in Figures 11(A) and 11(B), the relative cell viability of HaCaT cells was highest at 100 ppm, i.e., 100 μg / mL, and in the case of B16F10 cells, a concentration dependency was observed, where the higher the concentration, the higher the cell viability.

[0083] Figure 11(C) shows the DPPH radical scavenging ability of brewer's spent grains hot water extract at different concentrations of 5%, 15%, and 30%, respectively, and Figure 11(D) shows the polyphenol content measured for each of the same cases. The ascorbic acid concentration of the control standard solution in Figure 11(C) was 1 mg / mL, and the control standard solution in Figure 11(D) was gallic acid.

[0084] 11(C) and 11(D), the results show that the 100 μg / mL (100 ppm) extract concentration showed the highest polyphenol content and the highest DPPH radical inhibition ability. Furthermore, when classified by hot water extract concentration, the 15% extract showed the highest polyphenol content and the highest DPPH radical inhibition ability, followed by the 30% and 5% extracts.

[0085] Therefore, in order to maximize the polyphenol content and the melanin secretion inhibitory effect according to the target efficacy and effect, it was confirmed that it is most preferable to set the extraction concentration of brewer's spent grains in hot water extraction according to an embodiment of the present invention to 15% and mix the processed composition concentration at 100 μg / mL.

[0086] Meanwhile, FIG. 12 shows an analysis chart of the influence of processed brewers' grains using the brewers' grain extract according to an embodiment of the present invention on hair growth / hair development-related growth factors in human hair dermal papilla cells at different processing concentrations.

[0087] Referring to Figure 12, the cells used in this experiment were human hair dermal papilla cells (HPCs). The mRNA expression levels of growth factors in processed products using each brewer's spent grain extract at different processing concentrations were measured using qPCR. Three biomarkers were used: VEGF (involved in the proliferation of human hair dermal papilla cells), Wnt7b (involved in hair follicle stem cell homeostasis and hair growth cycling), and FGF7 (involved in the proliferation of human hair dermal papilla cells). For details on this efficacy analysis method, please refer to the 2018 paper, "Effect of Cinnamomum osmophloeum Kanehira Leaf Aqueous Extract on Dermal Papilla Cell Proliferation and Hair Growth" (Cell Transplant. 2018 Feb;27(2):256-263, Tung-Chou Wen et al.).

[0088] First, Figures 12(A), (B), and (C) show the results of the treatment of HDPCs (Human Hair Dermal Papilla Cells) at a cell concentration of 2 × 10 5 The results were obtained by reacting processed brewers' lees extracted with hot water at a concentration of 15% with 1000 cells / mL of minoxidil. After 24 hours of starvation and 24 hours of prep time, the biomarker values ​​confirmed for each processing concentration were shown. Additionally, a positive control was used, using minoxidil (MXD) at a concentration of 209.25 ng / mL (1 μM), which is known to have the highest effect on dermal papilla cells (DPCs).

[0089] 12(A), it can be seen that the expression level of VEGF at a treatment concentration of 100 μg / mL is significantly increased compared to other concentrations, with a 5-fold increase compared to the control and a significant increase of about 1.5-fold compared to the positive control.

[0090] 12(B), it can be seen that the expression level of Wnt7b at a treatment concentration of 100 μg / mL is significantly increased compared to other concentrations, with a 13.4-fold increase compared to the control and a significant increase of approximately 5.4-fold compared to the positive control.

[0091] 12(C), it can be seen that the expression level of FGF7 at a treatment concentration of 100 μg / mL is significantly increased compared to other concentrations, with a 7-fold increase compared to the control and a significant increase of approximately 1.2-fold compared to the positive control.

[0092] Also, Figures 12(D), (E), and (F) show the results for HDPCs (Human Hair Dermal Papilla Cells) tested on different days, with a cell concentration of 3 × 10 5 The results were obtained by incubating cells / dishes with brewers' lees extracted with hot water at a 15% extract concentration. 24 hours of starvation was required, and the biomarker values ​​observed for each concentration were shown. Similarly, a positive control was used, using minoxidil (MXD) at 209.25ng / mL (1μM), which is known to have the highest effect on dermal papilla cells (DPCs).

[0093] Referring to Figure 12(D), it can be seen that the expression level of VEGF at a treatment concentration of 200 μg / mL was significantly increased compared to other concentrations, with an approximately 11-fold increase compared to the control, demonstrating a significant difference from the positive control.

[0094] 12(E), it can be seen that the expression level of Wnt7b at a treatment concentration of 200 μg / mL was significantly increased compared to other concentrations, with a 5-fold increase compared to the control and a significant difference from the positive control.

[0095] 12(F), it can be seen that the expression level of FGF7 at a treatment concentration of 200 μg / mL was significantly increased compared to other concentrations, with a three-fold increase compared to the control and a significant difference from the positive control.

[0096] Based on these experimental results, it can be seen that in the case of brewer's lees processed product with a hot water extraction concentration of 15%, the highest ankle / hair growth-related efficacy of dermal papilla cells was observed at a processing concentration of 100μg / mL to 200μg / mL.

[0097] While specific embodiments of the present invention have been described above, it goes without saying that various modifications can be made without departing from the scope of the present invention. Therefore, the scope of the present invention should not be limited to the described embodiments, but should be defined by the following claims and their equivalents.

[0098] Although the present invention has been described above using limited embodiments and drawings, the present invention is not limited to the above embodiments, and various modifications and variations can be made by those skilled in the art based on such descriptions. Therefore, the spirit of the present invention should be understood only by the following claims, and equivalent or similar modifications should also be considered to fall within the spirit of the present invention.

Claims

1. Sterilizing the brewer's spent grain raw material; selectively drying the sterilized brewers' spent grain raw material according to a predefined drying environment; A step of crushing the dried beer spent material; Classifying the pulverized beer spent raw material according to particle size conditions; determining a post-processing method corresponding to the classified particle size conditions; A step of producing a processed beer spent product for producing a category-specific cosmetic composition according to the determined post-processing method; Including, The particle size conditions correspond to the grinding size conditions, The pulverization size conditions include at least one of a 50 mesh or smaller condition, a 50 mesh condition, a 1000 mesh condition, and a 3000 mesh condition, When the grinding size condition is 50 mesh or less, The post-processing method includes pulverizing the pulverized brewers' spent material, mixing the pulverized brewers' spent material with capsule seeds to granulate the pulverized brewers' spent material, and then coating and encapsulating the granulated brewers' spent material, The encapsulated beer spent material is used as a base formulation of the cosmetic composition, When the grinding size condition is 3000 mesh, the category of the cosmetic composition is a body scrub; When the grinding size condition is 1000 mesh, the category of the cosmetic composition is a body cleanser; When the grinding size condition is 50 mesh, the category of the cosmetic composition is a face scrub. A method for producing a processed brewer's spent grains product for producing a cosmetic composition, comprising:

2. The manufacturing category of the cosmetic composition varies depending on the grinding size conditions, The product category includes any of body scrubs, face scrubs, cleansers, scalp scaling products, body washes, facial washes, and shampoos. A method for producing a processed brewer's spent grains product for producing the cosmetic composition according to claim 1.

3. 2. The method for producing a processed brewers' spent grain for producing a cosmetic composition according to claim 1, wherein the post-processing method comprises obtaining a brewers' spent grain extract by performing hot water extraction and filtration on the crushed brewers' spent grain raw material.

4. The acquiring process includes:

4. A method for producing a processed brewers' spent grains for producing the cosmetic composition according to claim 3, comprising the steps of: mixing the ground brewers' spent grains raw material with distilled water at an extract concentration of 15% to 30% to prepare a mixture for hot water extraction; and performing hot water extraction from the mixture for hot water extraction to obtain the brewers' spent grains extract.

5. The cosmetic composition comprises: The crushed brewer's spent grains raw material is mixed with distilled water at an extraction concentration of 15% to produce a mixture for hot water extraction; The brewer's lees extract extracted with hot water from the mixture for hot water extraction is mixed with distilled water to a processing concentration of 100 ppm or 200 ppm to produce a processed brewer's lees product as an active ingredient, thereby providing a brewer's lees extract-containing cosmetic product having a hair growth or hair care effect on human hair papilla cells. The processing concentration means a weight-to-volume ratio (ppm) when the brewer's spent grain extract obtained by the process according to claim 4 is mixed with distilled water. A method for producing a processed brewer's spent grain product for producing the cosmetic composition according to claim 4.

6. The cosmetic composition comprises: The hot water extracted brewer's lees extract is mixed with distilled water to a processing concentration of 100 ppm to produce a processed brewer's lees product containing the brewer's lees extract as an active ingredient, thereby producing a brewer's lees extract-containing cosmetic product having antibacterial, whitening, antioxidant and melanin production inhibitory effects. The processing concentration means a weight-to-volume ratio (ppm) when the brewer's spent grain extract obtained by the process according to claim 4 is mixed with distilled water. A method for producing a processed brewer's spent grain product for producing the cosmetic composition according to claim 4.

7. Sterilizing the brewer's spent grain raw material; drying the sterilized brewers' spent grain raw material and pulverizing it into brewers' spent grain raw material powder; a step of pulverizing the pulverized brewers' spent grain raw material powder to a predetermined size or less to obtain brewers' spent grain fine powder; A step of preparing a capsule seed containing a capsule polymer for producing cosmetics that can contain the beer spent fine powder; Mixing the capsule seeds and the beer spent fine powder to obtain beer spent granules; an encapsulation step of coating the beer spent grain granules to encapsulate them; and obtaining the encapsulated brewers' spent material as a brewers' spent material for use as a base formulation of a cosmetic composition.

8. The basic formulation of the cosmetic composition is 8. A method for producing a processed brewer's lees product for producing the cosmetic composition according to claim 7, which is used as a basic formulation of a basic cosmetic product including at least one of a lotion, skin lotion, cream, massage cream, essence, and mask pack.

9. The pulverizing step includes: Selectively creating a dry environment according to a drying method; 8. The method for producing a processed brewer's spent grains for producing a cosmetic composition according to claim 7, wherein the drying environment is selected from the group consisting of an environment in which the processed brewer's spent grains are dried for a certain period of time at a first temperature of 40°C or higher, an environment in which freeze-drying is performed for a first period of time and then drying for a second period of time that is shorter than the first period of time at a second temperature of 70°C or higher, and an environment in which hot air drying is performed for a certain period of time.

10. The method for producing a processed brewer's spent grains for producing a cosmetic composition according to claim 9, wherein the drying environment is determined differently depending on the target polyphenol content and the target antioxidant capacity according to the characteristics of the cosmetic composition.

11. Sterilizing, drying and pulverizing the beer spent material; The crushed brewer's spent material is classified according to particle size requirements, and then processed in a post-processing manner to prepare brewer's spent scrub or brewer's spent capsules in advance; Separately measuring and preparing an aqueous phase and an oil phase of cosmetic raw materials for producing a cosmetic composition containing the beer spent scrub or beer spent capsules; a step of heating the aqueous phase and the oily phase in a hot water bath, and then emulsifying the aqueous phase by stirring the aqueous phase and adding the oily phase; cooling the emulsified composition; Adding the previously prepared brewer's spent grain scrub or brewer's spent grain capsules and stirring; and subjecting the stirred composition to a vacuum degassing treatment.

12. The particle size conditions correspond to the grinding size conditions, 12. The method for producing a cosmetic composition containing a brewers' spent grain extract according to claim 11, wherein the grinding size conditions include at least one of a condition of 50 mesh or less, a condition of 50 mesh, a condition of 1000 mesh, and a condition of 3000 mesh.

13. The cosmetic composition is produced in different production categories depending on the grinding size conditions, 13. The method for producing a cosmetic composition containing brewers' spent grain extract according to claim 12, wherein the product categories include any of body scrubs, face scrubs, cleansers, scalp scaling products, body washes, facial washes, and shampoos.

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