Amplification module having a gas transfer path and an extract transfer path

Abstract

The present invention relates to an amplification module having a gas transfer passage and an extract transfer passage, and more specifically, to an amplification module in which a fixed amount of extract is dispensed into each storage section when extract is dispensed from an extraction device in which the amplification module is installed, thereby improving detection accuracy.

Description

[Technical Field]

[0001] The present invention relates to an amplification module having a gas transfer passage and an extract transfer passage. [Background technology]

[0002] In modern times, advances in biotechnology have made it possible to interpret the causes of disease at the genetic level, which has led to an ever-increasing demand for the manipulation and biochemical analysis of biological samples to cure or prevent human diseases.

[0003] In addition to disease diagnosis, there is a demand for technology to extract and analyze nucleic acids from biological samples and samples containing cells in a variety of fields, including new drug development, preliminary testing for viral and bacterial infections, and forensic medicine.

[0004] Conventional genome extraction equipment requires separate equipment for each processing step (concentration, purification), and after one processing step is completed, the sample must be moved to another device, which takes a long time.

[0005] In order to solve the conventional problem of low detection efficiency during such a long processing process, the present applicant has developed and used patents such as Patent No. 10-1989920.

[0006] In the above document, the buffer is directly dispensed into the buffer chamber and stored, but there is a problem that micro-leakage occurs through the various layer structures at the bottom of the buffer chamber during long-term storage, adversely affecting extraction performance.

[0007] In addition, the pad placed between the upper body and the base plate is made of rubber, but as the pad is pressed between the upper body and the base plate, the diameter of the hole formed through the pad decreases, causing a problem in which a volume of extract different from the intended volume of the product design moves to the amplification module.

[0008] Furthermore, due to vibrations occurring during the production and distribution of the product, the sealing member sealing the buffer chamber may be punctured by the protruding member, resulting in leakage and contamination of the reagent stored inside the buffer chamber.

[0009] Therefore, the present inventors focused on and completed the present invention in order to solve the problems of conventional genome extraction devices. [Prior art documents] [Patent documents]

[0010] [Patent Document 1] Korean Patent Registration No. 10-1989920 [Patent Document 2] Korean Patent Registration No. 10-2065649 [Patent Document 3] Korean Patent Registration No. 10-2065650 [Patent Document 4] Korean Patent Registration No. 10-2076220 Summary of the Invention [Problem to be solved by the invention]

[0011] According to the present invention, an inner chamber containing reagents necessary for genome extraction is provided separately from an outer chamber, and the upper and lower parts of the inner chamber are sealed, thereby solving the problem of reagents contained in a single chamber leaking out in conventional genome extraction devices.

[0012] Another object of the present invention is to provide a genome extraction device including a safety clip to prevent the protruding members formed on the cover and outer chamber from puncturing the sealing members that seal the upper and lower openings of the inner chamber due to the inner chamber moving up and down due to vibrations that occur during the production and distribution process of the product.

[0013] Another objective of the present invention is to provide a genome extraction device that solves the problem of cross-contamination between reagents due to capillary action occurring through the space between the double chambers through a unique inner chamber design (lower inner chamber).

[0014] Another objective is to provide a genome extraction device that has a unique inner chamber design (upper inner chamber) to prevent reagents from leaking out in a structure that prevents capillary action.

[0015] Another object of the present invention is to provide a genome extraction device in which the sealing member can be broken with little force due to the configuration of the first protruding member formed on the bottom surface of the outer chamber, and the perforated portion expands, allowing the reagent contained inside the inner chamber to smoothly flow out to the outside.

[0016] Another object of the present invention is to provide a genome extraction device in which a sloped portion is formed around a discharge hole through which a reagent is discharged, thereby enabling the reagent to be smoothly discharged through the discharge hole.

[0017] In addition, the purpose of this invention is to provide a genome extraction device that is easier to manufacture and solves the problem of unintentional narrowing of the flow path, by arranging a double-structured flow cover-pad between the outer chamber and the base plate, compared to conventional genome extraction devices that have only one pad arranged.

[0018] Another object of the present invention is to provide a genome extraction device in which a tightly sealed flow path is formed without intermediate leakage during the reagent transfer process by achieving a strong engagement between the base plate, flow cover, pad, and outer chamber.

[0019] Another objective of the present invention is to provide a genome extraction device that can maintain the performance of beads, which are sensitive to moisture, for a long period of time by having a double chamber structure consisting of an outer chamber and a bead chamber in the bead chamber that contains the beads necessary for genome extraction and amplification.

[0020] Another object of the present invention is to provide a genome extraction device in which the performance of the beads is maintained by a dehumidifying unit located above the bead chamber even when the bead chamber is opened.

[0021] Another object of the present invention is to provide a genome extraction device using an amplification module that allows a sufficient volume of extract to be introduced by easily expelling any air remaining inside the storage section as the pretreated extract is introduced.

[0022] In addition, the amplification module has multiple storage compartments, each of which stores different primers and probes for genome amplification, and the purpose is to provide a genome extraction device that can diagnose several types of diseases through a single genome extraction.

[0023] Another object of the present invention is to provide a genome extraction device in which the length, thickness, and pattern of the gas transfer passage and the extract transfer passage are different depending on the position of the connected storage section, thereby preventing the extraction or amplification product introduced into the storage section from mixing.

[0024] Another object of the present invention is to provide a genome extraction method using the genome extraction device described above. [Means for solving the problem]

[0025] In order to solve the above problems, one embodiment of the present invention provides an amplification module that engages with an extractor and stores an extract extracted from the extractor, the amplification module including: a body; one or more inlets formed on one side of the body and communicating with a discharge hole of the extractor; a storage section formed on the other side of the body as a space for storing the extract; a gas transfer passage formed on one side of the body and connecting one of the one or more inlets to the storage section; and an extract transfer passage formed on the opposite side of the one side of the body and connecting another of the one or more inlets to the storage section.

[0026] In one embodiment, the gas transfer passage may be connected to an upper part of the container, and the extract transfer passage may be connected to a lower part of the container.

[0027] In one embodiment, the edges of the container may be rounded.

[0028] In one embodiment, the gas transfer passage and the extract transfer passage may be bent one or more times from the inlet to the container, and the edges of the bent portions may be rounded.

[0029] In one embodiment, the receiving portion may have a shape in which the width thereof narrows as it moves away from the gas passage and the extract passage.

[0030] In one embodiment, the storage units may be provided in a plurality, the gas transfer passage may be formed by extending from one of the inlets so as to be connected to the storage units in a one-to-one correspondence, and the extract transfer passage may be formed by extending from another of the inlets so as to be connected to the storage units in a one-to-one correspondence.

[0031] In one embodiment, the plurality of gas transfer passages may be formed to have different lengths.

[0032] In one embodiment, the length of the gas transfer passage may be shorter as the gas transfer passage is connected to the receiving portion located at the top.

[0033] In one embodiment, the gas transfer passage includes a communication hole formed through the body and communicating with the storage unit, a transfer passage extending from the communication hole, a storage passage for storing a predetermined volume of gas, and a passage pattern forming part combined with one or more points of the transfer passage and closing the combined part of the transfer passage, and the more the gas transfer passage connected to the storage unit located at the upper position, the more the number of the storage passages and the number of points where the passage pattern forming part is combined may be.

[0034] In one embodiment, the plurality of extraction liquid transfer passages may be formed to have different lengths.

[0035] In one embodiment, the thickness of the extract transfer passages having different lengths may be thinner as the extract transfer passage connected to the receiving portion is located at the top.

[0036] In one embodiment, the extract transfer path may extend from the inlet to a single point and branch from the single point toward each of the storage portions.

[0037] In one embodiment, the thickness of the extract passage may vary from the branched point.

[0038] In one embodiment, the container may store a substance for amplifying a genome contained in the extract.

[0039] In one embodiment, a plurality of the containers may be provided, and different materials for amplifying genomes may be stored in each of the containers.

[0040] In one embodiment, the one surface and the opposite surface of the body may be sealed by a sealing member attached to the one surface and the opposite surface. [Effects of the Invention]

[0041] The genome extraction device according to the present invention has an inner chamber containing reagents necessary for genome extraction, which is separate from the outer chamber, and the top and bottom of the inner chamber are sealed, thereby solving the problem of reagents contained in a single chamber leaking out in conventional genome extraction devices.

[0042] In addition, when the inner chamber moves up and down due to vibrations that occur during the production and distribution process of the product, the protruding members formed on the cover and the outer chamber prevent the sealing members that seal the upper and lower openings of the inner chamber from being punctured.

[0043] Furthermore, the problem of cross-contamination between reagents is resolved by capillary action occurring through the space between the double chambers.

[0044] Furthermore, the structure for preventing capillary action prevents the reagent from leaking out.

[0045] In addition, the protruding member formed on the bottom surface of the outer chamber allows the sealing member to be broken with little force, expanding the perforated area and allowing the reagent contained inside the inner chamber to smoothly flow out.

[0046] In addition, a sloped portion is formed around the outlet hole through which the reagent is discharged, so that the reagent can be smoothly discharged through the outlet hole.

[0047] In addition, the dual-structure flow cover-pad is placed between the outer chamber and the base plate, which improves ease of manufacturing compared to conventional genome extraction devices that only have one pad, and solves the problem of unintentional narrowing of the flow path.

[0048] Furthermore, by achieving a strong engagement between the base plate, flow cover, pad and outer chamber, a sealed flow path is formed without intermediate leakage during the reagent transfer process.

[0049] In addition, the bead chamber containing the beads necessary for genome extraction and amplification has a double chamber structure consisting of an outer chamber and a bead chamber, which makes it possible to maintain the performance of the beads, which are sensitive to moisture, for a long period of time.

[0050] In addition, even if the bead chamber is opened, the performance of the bead is maintained by the dehumidifying unit located above the bead chamber.

[0051] Furthermore, by adding the pretreated extract, any air remaining inside the container can be easily expelled, and a sufficient volume of extract can be added to the amplification module.

[0052] In addition, the amplification module has a plurality of storage compartments, each of which stores different primers and probes for genome amplification, making it possible to diagnose several types of diseases through a single genome extraction.

[0053] In addition, the length, thickness, and pattern of the gas transfer passage and the extract transfer passage are different depending on the position of the connected storage section, thereby preventing the extract or amplification product introduced into the storage section from being mixed. [Brief explanation of the drawings]

[0054] [Figure 1] 1 is a perspective view showing the overall appearance of a genome extraction device according to an embodiment of the present invention. [Figure 2] FIG. 2 is a perspective view of the genome extraction device of FIG. 1 as seen from another side. [Figure 3] FIG. 2 is an exploded perspective view of FIG. 1. [Figure 4] 10 is a diagram illustrating an engagement relationship between an outer chamber and an inner chamber. [Figure 5] 10 is a diagram illustrating an engagement relationship between an inner chamber and a safety clip. [Figure 6] FIG. 1 is a plan view of the outer chamber. [Figure 7] FIG. 10 is a cross-sectional view illustrating the engagement relationship between the inner chamber and the outer chamber. [Figure 8] 10 is an enlarged view illustrating a protruding member formed on the bottom surface of an outer chamber. [Figure 9] 1 is a diagram for explaining the inner chamber in more detail. [Figure 10] FIG. 10 is a bottom perspective view for more specifically illustrating the cover. [Figure 11] FIG. 10 is an exploded perspective view for more specifically illustrating the flow cover and pad disposed between the base plate and the outer chamber. [Figure 12] FIG. 2 is an exploded perspective view for specifically explaining the configuration of the piston. [Figure 13] FIG. [Figure 14] FIG. 4 is a perspective view for more specifically explaining the base plate. [Figure 15] 1 is a cross-sectional view for specifically explaining a genome extraction device according to an embodiment of the present invention. [Figure 16] FIG. 2 is another cross-sectional view for specifically explaining the genome extraction device according to an embodiment of the present invention. [Figure 17] 1 is a diagram illustrating an amplification module according to a first embodiment of the present invention. [Figure 18] 1 is a diagram illustrating an amplification module according to a first embodiment of the present invention. [Figure 19] 1 is a diagram illustrating an amplification module according to a first embodiment of the present invention. [Figure 20] 10 is a diagram illustrating an amplification module according to a second embodiment of the present invention. [Figure 21] 10 is a diagram illustrating an amplification module according to a second embodiment of the present invention. [Figure 22] 10 is a diagram illustrating an amplification module according to a second embodiment of the present invention. [Figure 23] 10 is a diagram illustrating an amplification module according to a third embodiment of the present invention. [Figure 24] 10 is a diagram illustrating an amplification module according to a third embodiment of the present invention. [Figure 25] 10 is a diagram illustrating an amplification module according to a third embodiment of the present invention. [Figure 26] FIG. 2 is a plan view of the bead chamber. [Figure 27] FIG. 2 is a perspective view for more specifically explaining the configuration of a bead chamber. [Figure 28] FIG. 2 is a perspective view for more specifically explaining the configuration of a bead chamber. [Figure 29] FIG. 29 is a cross-sectional view of the bead chamber of FIG. 28. [Figure 30]29 is a longitudinal cross-sectional view of the bead chamber of FIG. 28, illustrating the structure engaged with the outer chamber. DETAILED DESCRIPTION OF THE INVENTION

[0055] In some cases, well-known structures and devices are omitted or shown in block diagram form, focusing on the core functions of each structure and device, in order to avoid obscuring the concepts of the present invention.

[0056] Throughout the specification, when a part "comprising" or "including" a certain element(s), this does not mean excluding other elements, but means further including other elements, unless otherwise specified to the contrary. Furthermore, terms such as "unit," "group," and "module" used in the specification refer to a unit that processes at least one function or operation, and may be embodied in hardware, software, or a combination of hardware and software. Furthermore, in the context of describing the present invention (particularly in the context of the following claims), the terms "a" (or "an"), "one," "the," and similar related words are used to include both the singular and the plural, unless otherwise indicated in the specification or clearly contradicted by the context.

[0057] In describing embodiments of the present invention, if a detailed description of well-known functions or configurations is deemed to unnecessarily obscure the gist of the present invention, the detailed description will be omitted. Furthermore, the terms used below are defined in consideration of the functions in the embodiments of the present invention, and may vary depending on the intentions or practices of users or operators. Therefore, the definitions should be based on the overall content of this specification.

[0058] The present invention will now be described in detail with reference to the accompanying drawings.

[0059] 1 and 2, a genome extraction device 1000 according to an embodiment of the present invention includes an outer chamber 100, an inner chamber 200, a cover 300, a base plate 400, a safety clip 500, an amplification module 600, a piston 700, a drive unit 800, and a bead chamber 900.

[0060] The outer chamber 100 is partitioned by outer chamber partitions into a plurality of first spaces 101, 102, 103, 104, 105, 106, and 107. That is, the plurality of first spaces 101, 102, 103, 104, 105, 106, and 107 may be spaces independent from one another.

[0061] The first spaces 101, 102, 103, 104, 105, 106, and 107 may have an open top and a closed bottom. First discharge holes 121, 122, 123, 124, and 125 are formed through the bottoms of the first spaces 101, 102, 103, 104, and 105, respectively, and are spaced a first distance from the center of the outer chamber 100 and extend in the circumferential direction. Second discharge holes 126 and 127 are formed through the bottoms of the remaining first spaces 106 and 107, respectively, and are spaced a second distance from the center of the outer chamber 100 and extend in the circumferential direction. Discharge holes 128 and 129, which communicate with the amplification module 600, are formed through the bottoms of the spaces between the first spaces 106 and 107. Here, the first distance may be shorter than the second distance, but in other embodiments, the first distance may be longer than the second distance.

[0062] A reagent stored in an inner chamber 200 (described later) is poured into the plurality of first spaces 101, 102, 103, 104, and 105, and beads stored in a bead chamber 900 are poured into the remaining plurality of first spaces 106 and 107.

[0063] Piston insertion portions 108 into which pistons 700 are inserted are formed penetrating vertically at the centers of the plurality of first spaces 101, 102, 103, 104, 105, 106, and 107. When the pistons 700 are inserted into the piston insertion portions 108 and a driving portion (not shown) of the diagnostic device engages with the pistons 700 to raise and lower the pistons 700, the reagent (fluid) in the first spaces 101, 102, 103, 104, 105, 106, and 107 can enter and exit the fluid containing portion 701 inside the pistons 700. More specific details will be described later.

[0064] 4, the upper outer surface 100a of the outer chamber 100 is connected to the upper portion of the lower outer surface 100b and recessed toward the center. The safety clip 500 engages with the upper outer surface 100a of the outer chamber 100, and the boundary between the upper outer surface 100a and the lower outer surface 100b serves as a short jaw of the safety clip 500, so that the safety clip 500 can maintain its engagement position after engaging with the upper outer surface 100a. The safety clip 500 has a length that at least partially surrounds the periphery of the upper outer surface 100a of the outer chamber 100 and includes an extending outer chamber engaging portion 510 and a handle 520 formed on one side of the outer chamber engaging portion 510.

[0065] By engaging the safety clip 500 with the outer chamber 100, the cover 300 can pressurize the inner chamber 200, which is engaged with the outer chamber 100, preventing the upper and lower openings of the inner chamber 200 from opening. The user can begin the extraction process after grasping the handle 520 and removing the safety clip 500 from the outer chamber 100. In other words, when the safety clip 500 is engaged with the outer chamber 100, the reagent in the inner chamber 200 cannot be introduced into the outer chamber 100, and only when the safety clip 500 is removed from the outer chamber 100 can the reagent in the inner chamber 200 be introduced into the outer chamber 100.

[0066] 3-5, the construction of the safety clip 500 will be described in more detail.

[0067] Safety clip 500 includes an outer chamber engaging portion 510 , a handle 520 , a top extension 530 and a side extension 540 .

[0068] The outer chamber engaging portion 510 engages with the outer chamber 100 while surrounding at least a portion of the periphery of the outer surface (specifically, the upper outer surface 100a) of the outer chamber 100. More specifically, the outer chamber engaging portion 510 engages with the outer chamber 100 so as to surround the four outer surfaces of the outer chamber 100, but the extended ends of the outer chamber engaging portion 510 may be configured to be spaced apart from each other. As shown in FIG. 1 , when the safety clip 500 engages with the outer chamber 100, the extended end of the outer chamber engaging portion 510 rests on one of the outer surfaces of the outer chamber 100, and the safety clip 500 can be separated from the outer chamber 100 only when the user grasps the safety clip 500 and applies an external force in one direction.

[0069] The handle 520 is a portion that extends outward from the outer chamber engagement portion 510 and is the portion that is grasped by the user to separate the safety clip 500 from the outer chamber 100 .

[0070] The upper extension 530 extends upward from one side of the outer chamber engaging portion 510 , and the side extension 540 extends from the upper extension 530 toward the center of the outer chamber 100 .

[0071] The safety clip 500 according to the embodiment of the present invention is characterized in that a cover support member 541 is protruded from the upper surface of the side extension portion 540, and an inner chamber engagement portion 542 is protruded from the extension end of the side extension portion 540.

[0072] The cover support member 541 serves to prevent the protruding members 311, 312, 313, 314, 315, 316, 317 formed on the bottom surface of the cover 300 from breaking (puncturing) the first sealing member S1 that seals the upper openings of the multiple second spaces 201, 202, 203, 204, 205 of the inner chamber 200 and the third sealing member S3 that seals the upper opening of the bead chamber 900 when the safety clip 500 engages with the outer chamber 100.

[0073] 15, when the safety clip 500 is engaged with the outer chamber 100 and the inner chamber 200, contact between the protruding members 311, 312, 313, 314, 315, 316, and 317 and the first and third sealing members S1 and S3 is blocked. Therefore, when the safety clip 500 is engaged with the outer chamber 100 and the inner chamber 200, perforation of the inner chamber 200 and the bead chamber 900 is prevented, thereby preventing the reagent contained in the inner chamber 200 and the beads contained in the bead chamber 900 from leaking into the outer chamber 100.

[0074] The inner chamber engaging portion 542 is a portion that engages with the fixing portion 230 of the inner chamber 200 when the safety clip 500 engages with the outer chamber 100. When the inner chamber engaging portion 542 engages with the fixing portion 230, the bottom surface of the inner chamber 200 is positioned at a predetermined distance from the bottom surface of the outer chamber 100, and therefore, the second sealing member S2 that seals the lower openings of the plurality of second spaces 201, 202, 203, 204, 205 is prevented from being torn by the protruding members 111, 112, 113, 114, 115 formed on the bottom surface of the outer chamber 100 (see FIG. 15).

[0075] In the attached drawings, the inner chamber engaging portion 542 is shown in the form of an engaging protrusion and the fixing portion 230 is shown in the form of an engaging groove that engages with the engaging protrusion, but in other embodiments, the inner chamber engaging portion 542 may be provided in the form of an engaging groove and the fixing portion 230 may be provided in the form of an engaging protrusion that engages with the engaging groove.

[0076] The outer chamber 100 (more specifically, the outer chamber partition) has a recessed seating portion 109 that provides a space for the fixing portion 230 of the inner chamber 200 to be seated. 500 The inner chamber 200 is fixed at a position spaced a predetermined distance from the bottom of the outer chamber 100 through an engagement structure with the seat 109, and the fixing force can be further improved by the fixing part 230 of the inner chamber 200 being seated and supported by the seat 109.

[0077] 7, an insertion space 130 is recessed into the upper side of the inner wall of the outer chamber 100, and an engagement hook 240 of the inner chamber 200 can engage with the insertion space 130. A stopper 131 is formed on the upper side of the insertion space 130 and protrudes toward the inside of the outer chamber 100. Therefore, when the inner chamber 200 is not pressurized by the cover 300, the engagement hook 240 of the inner chamber 200 is positioned on the stopper 131, but when the inner chamber 200 is pressurized by the cover 300, the engagement hook 240 may pass through the stopper 131 and be inserted into the insertion space 130.

[0078] Referring to Figure 15, the engagement relationship between the outer chamber and the inner chamber according to another embodiment of the present invention will be described. In Figure 7, instead of the engagement hook 240 formed on the inner chamber 200, a locking protrusion 250 is provided that protrudes outward from the outer wall of the inner chamber 200, and the locking protrusion 250 engages with a stopper 131 formed on the inner wall of the outer chamber 100, partially restricting the downward movement of the inner chamber 200. When the safety clip 500 is removed from the outer chamber 100 and the inner chamber 200 is pressurized by the cover 300, the locking protrusion 250 passes through the stopper 131 and is inserted into the insertion space 130, thereby piercing the second sealing member S2 that seals the multiple second spaces in the inner chamber 200 by the protruding member formed on the outer chamber 100.

[0079] The inner chamber 200 is partitioned by inner chamber partitions into a plurality of second spaces 201, 202, 203, 204, and 205. That is, the plurality of second spaces 201, 202, 203, 204, and 205 may be spaces independent from one another.

[0080] The upper and lower portions of the plurality of second spaces 201, 202, 203, 204, and 205 are open (i.e., the plurality of second spaces have upper and lower openings), and the upper portions are sealed by a first sealing member S1, and the lower portions are sealed by a second sealing member S2. The first sealing member S1 and the second sealing member S2 may be, for example, a film, but are not limited thereto, and a film made of any material that is impermeable to fluids may be used.

[0081] Different reagents are poured into the multiple second spaces 201, 202, 203, 204, and 205, respectively. First, the second sealing member S2 seals the lower portions of the multiple second spaces, and then the reagents are poured into the inner chamber 200. Then, the first sealing member S1 seals the upper portions of the multiple second spaces, thereby completing the pouring of the reagents into the inner chamber 200.

[0082] Referring to FIG. 4, the inner chamber 200 includes an upper inner chamber 210 and a lower inner chamber 220 .

[0083] The upper inner chamber 210 is integrally formed and is configured to fit tightly against the inner wall of the outer chamber 100 when engaged with the outer chamber 100 .

[0084] The lower inner chamber 220 is connected to the upper inner chamber 210 and includes a portion that is bent (toward the inside in the radial direction) so as to be separated from the inner wall of the outer chamber 100 when engaged with the outer chamber 100 .

[0085] In the present invention, since a dual chamber structure consisting of an inner chamber and an outer chamber is used, there is a risk of cross-contamination between reagents in the inner chamber 200 during operation. Cross-contamination occurs when capillary action occurs through the minute space between the inner and outer chambers. In order to prevent this cross-contamination problem, the present invention employs a curved structure in which the inner chamber 200 is sufficiently spaced from the inner wall of the outer chamber 100 to prevent capillary action.

[0086] In addition, the outer chamber 100 and the inner chamber 200 are designed to be separated to prevent capillary action, and the upper inner chamber 210 is configured to be in close contact with the inner wall of the outer chamber 100 to prevent the reagent from leaking out through the separated area.

[0087] Meanwhile, second protruding members 111, 112, 113, 114, and 115 are protrudingly formed on the bottom surfaces of the first spaces 101, 102, 103, 104, and 105, so that the reagent contained in the inner chamber 200 can flow into the first spaces 101, 102, 103, 104, and 105 by breaking the second sealing member S2 of the inner chamber 200.

[0088] Each second protruding member 111, 112, 113, 114, 115 may be arranged in one-to-one correspondence with the plurality of first spaces 101, 102, 103, 104, 105, and for example, the second protruding member corresponding to drawing reference symbol 111 breaks the second sealing member S2 sealing the lower part of the second space corresponding to drawing reference symbol 201, and the second protruding member corresponding to drawing reference symbol 115 breaks the second sealing member S2 sealing the lower part of the second space corresponding to drawing reference symbol 205.

[0089] The second protruding members 111, 112, 113, 114, 115 include protruding portions 111a, 112a, 113a, 114a, 115a that protrude a first height (h1) from the bottom surfaces of the plurality of first spaces 101, 102, 103, 104, 105, and wing portions 111b, 112b, 113b, 114b, 115b that extend from the protruding portions 111a, 112a, 113a, 114a, 115a and protrude a second height h2 that is lower than the first height h1 from the bottom surfaces. Here, the wing portions 111b, 112b, 113b, 114b, 115b may have a structure that extends in both the left and right directions from the protruding portions 111a, 112a, 113a, 114a, 115a.

[0090] The protrusion serves to pierce the second sealing member S2, and the wing serves to expand the perforation of the second sealing member S2. In the present invention, since the height of the protrusion is higher than the wing, point contact is made between the second sealing member S2, which seals the lower part of the inner chamber 200, and the protrusion. This point contact has the effect of minimizing pressure when the second sealing member S2 is torn. Therefore, the second sealing member S2 can be torn with less force.

[0091] When the second sealing member S2 is torn by the protruding members 111, 112, 113, 114, and 115, the reagent stored in the second spaces 201, 202, 203, 204, and 205 of the inner chamber 200 flows out into the first spaces 101, 102, 103, 104, and 105 of the outer chamber 100. The leaked reagent is then discharged through the first discharge holes 121, 122, 123, 124, and 125 formed on the bottom surfaces of the first spaces 101, 102, 103, 104, and 105. In order to facilitate the outflow of the reagent to the first discharge holes 121, 122, 123, 124, and 125, there are portions around the first discharge holes 121, 122, 123, 124, and 125 that are inclined downward toward the first discharge holes 121, 122, 123, 124, and 125. The inclined portions may have an angle of 3 to 10 degrees, which facilitates the process of the reagent that has flowed into the first spaces 101, 102, 103, 104, and 105 flowing out to the first discharge holes 121, 122, 123, 124, and 125.

[0092] The cover 300 is configured to engage with the top of the outer chamber 100 and cover the top of the outer chamber 100 and the inner chamber 200 .

[0093] Referring to FIG. 10, the cover 300 includes a cover body 301 and a lid 302 .

[0094] The cover body 301 is formed with a first insertion hole 307 aligned with the piston insertion portion 108 and a first specimen insertion hole 309 through which the specimen is inserted (in the attached drawing, the first specimen insertion hole 309 is aligned with the first space 105, and the specimen is inserted into the first space 105 through the first specimen insertion hole 309), and the bottom surface of the cover body 301 is formed with first protruding members 311, 312, 313, 314, 315 for breaking the first sealing member S1 and third protruding members 316, 317 for breaking the third sealing member S3.

[0095] The first protruding members 311, 312, 313, 314, and 315 may be arranged in one-to-one correspondence with the plurality of first spaces 101, 102, 103, 104, 105, 106, and 107, and the third protruding members 316 and 317 may be arranged in one-to-one correspondence with the plurality of third spaces 910 and 920. For example, the first protruding member corresponding to reference numeral 311 breaks the first sealing member S1 sealing the upper part of the second space corresponding to reference numeral 201, and the first protruding member corresponding to reference numeral 315 breaks the first sealing member S1 sealing the upper part of the second space corresponding to reference numeral 205.

[0096] A spacing member 320 is formed on the bottom surface of the cover body 301 along the periphery of the first insertion hole 307. The spacing member 320 separates the first protruding member and the first sealing member from each other when the safety clip 500 is engaged with the outer chamber 100. That is, the spacing member 320 is supported by the cover support member 541, so that the cover 300 is spaced a predetermined distance from the inner chamber 100.

[0097] The cover 302 is hingedly connected to one side of the cover body 301. A second insertion hole 308 aligned with the first insertion hole 307 is formed through the center of the cover 302.

[0098] With the cover 300 engaged with the outer chamber 100, after the safety clip 500 is separated from the outer chamber 100, pressing the cover 300 downward causes the inner chamber 200 engaged with the outer chamber 100 to descend along the inner wall of the outer chamber 100. Second protruding members 111, 112, 113, 114, 115, 116, and 117 are formed on the bottom surface of the outer chamber 100, and first protruding members 311, 312, 313, 314, and 315 and third protruding members 316 and 317 are formed on the bottom surface of the cover 300, causing the first sealing member S1 and second sealing member S2 sealing the upper and lower openings of the inner chamber 200, and the third sealing member S3 sealing the upper opening of the bead chamber 900, to tear. Therefore, the reagent contained in the inner chamber 200 flows out into the multiple first spaces 101, 102, 103, 104, and 105 of the outer chamber 100, and the second sealing member S2 sealing the upper opening of the inner chamber 200 tears, thereby acting as an air vent to ensure that the reagent is sufficiently discharged into the first spaces.

[0099] The base plate 400 is engaged with the lower part of the outer chamber 100 and includes a plurality of channels that guide the path of the reagent between the first spaces 101 , 102 , 103 , 104 , 105 , 106 , 107 of the outer chamber 100 and the fluid-containing portion of the piston 700 .

[0100] According to one embodiment of the present invention, the base plate 400 has liquid flow paths 401 to 408 through which liquid can move and an air flow path 409 through which air can move, and a flow cover 410 and a pad 420 may be further provided between the outer chamber 100 and the base plate 400 to prevent leakage of liquid when the outer chamber 100 is engaged. When the base plate 400, the flow cover 410, and the pad 420 are engaged, the upper surfaces of the liquid flow paths and the air flow paths of the base plate 400 are blocked by the flow cover 410 and the pad 420, forming a space and completing the flow paths.

[0101] The liquid flow paths 401 to 408 are connected to the flow cover 410, the pad 420 and the outer chamber 100, and provide a space in which the specimen and the reagent can move and mix.

[0102] The air flow path 409 connects the amplification module 600 and the vacuum control portion of the piston 700, and serves to control the vacuum that may be generated when the genome extracted from the amplification module 600 moves, thereby preventing contamination of the amplification products that may be generated during genome amplification.

[0103] In one embodiment, one end of the air flow path 409 is connected to the fluid containing portion 701 of the piston 700, and the other end is connected to the amplification module 600, and air discharged from the amplification module 600 may pass through the air flow path 409 and be discharged to the fluid containing portion 701.

[0104] A plurality of flow channels 401, 402, 403, 404, 405, 406, 407, 408, and 409 are formed on the upper portion of the base plate 400. The flow channels do not intersect with each other. base plate The liquid flow passages are formed so as to extend from the center to the outer periphery of 400. Here, the liquid flow passages are components corresponding to reference numerals 401 to 408, and the air flow passage is a component corresponding to reference numeral 409.

[0105] 14, some of the multiple flow paths may have one end located on the same circumference and the other ends located on the same circumference. Among the multiple flow paths, one end of air flow path 409 is located on a different circumference from one end of the other liquid flow paths 401-408, and the other end is also located on a different circumference from the other ends of the other liquid flow paths 401-408, making it possible to control a vacuum.

[0106] A piston driver insertion hole 400a is formed through the center of the base plate 400 so that a piston driver 800 that rotates the piston 700 can be engaged therewith.

[0107] A flow cover 410 is placed in the mounting space on the upper part of the base plate 400. The flow cover 410 may be made of, for example, plastic, and may be ultrasonically fused to the upper part of the base plate 400 while being mounted thereon, thereby being integral with the base plate 400.

[0108] The flow cover 410 has a first through hole 410a aligned with the insertion hole 400a of the piston drive unit, a plurality of first flow cover holes 411a, 412a, 413a, 414a, 415a, 416a, 417a, and 418a formed on a first circumference at a first distance from the first through hole 410a, a plurality of second flow cover holes 411b, 412b, 413b, 414b, and 415b formed on a second circumference at a second distance from the first through hole 410a, a plurality of third flow cover holes 416b, 417b, and 418b formed on a third circumference at a third distance from the first through hole 410a, and fourth flow cover holes 419a and 419b communicating with one end and the other end of the air flow path 409. Here, the first flow cover holes are base plate The second flow cover hole is aligned with one inner end of the flow path formed in 400, the second flow cover hole and the third flow cover hole are aligned with the other outer ends of the flow path, and the fourth flow cover hole communicates with one end and the other end of the air flow path. The second distance may be longer than the first distance and shorter than the third distance.

[0109] Referring to FIG. 11, a first engaging protrusion 410b protruding upward and downward may be further formed on the outer periphery of the first through-hole 410a.

[0110] Furthermore, melt protrusions 410c may be formed on the bottom surface of the flow cover 410 to engage with the edges of a plurality of flow paths of the base plate 400 (see FIG. 12). When ultrasonic welding is performed after the flow cover 410 is placed on the top surface of the base plate 400, the melt protrusions 410c melt and become integrated with the base plate 400. This allows for tight engagement between the base plate 400 and the flow cover 410.

[0111] A pad 420 is placed on the upper part of the flow cover 410. The pad 420 may be made of, for example, a silicon material, but is not limited thereto and may be made of any material having a predetermined elasticity.

[0112] The flow cover 410 has a plurality of second engagement protrusions 410d formed protruding from the top surface thereof, and the second engagement protrusions 410d engage with the engagement grooves 420c of the pad 420, thereby achieving a strong engagement between the flow cover 410 and the pad 420. In addition, the first engagement protrusions 410b of the flow cover 410 are also inserted and coupled into the second through-holes 420a of the pad 420, thereby achieving a strong engagement between the two components.

[0113] The pad 420 has a second through hole 420a aligned with the first through hole 410a, and has a plurality of first pad holes 421a, 422a, 423a, 424a, 425a, 426a, 427a, and 428a formed therethrough on a first circumference a first distance away from the second through hole 420a, a plurality of second pad holes 421b, 422b, 423b, 424b, and 425b formed therethrough on a second circumference a second distance away from the second through hole 420a, a plurality of third pad holes 426b, 427b, and 428b formed therethrough on a third circumference a third distance away from the second through hole 420a, and fourth pad holes 429a and 429b communicating with one end and the other end of the air flow path 409 formed therethrough. Here, the first pad hole is aligned with the first flow cover hole, the second pad hole is aligned with the second flow cover hole, the third pad hole is aligned with the third flow cover hole, and the fourth pad hole is aligned with the fourth flow cover hole.

[0114] The pad 420 has a protrusion formed on its upper surface that protrudes from the area where the plurality of second pad holes 421b, 422b, 423b, 424b, and 425b, the plurality of third pad holes 426b, 427b, and 428b, and the fourth pad hole 429b, which communicates with the other end of the air flow path, are formed, and the protrusion narrows toward the top. The protrusion solves the problem of the diameter of the pad hole decreasing unintentionally even when the pad 420 is closely placed between the outer chamber 100 and the base plate 400.

[0115] The amplification module 600 is engaged with the outer chamber 100 and configured to receive a pre-treated sample. Pre-treatment of the sample means that genomes such as DNA and RNA contained in the sample have been lysed into a reagent. When the genome extraction device 1000 according to the present invention is engaged with a diagnostic device (not shown), an amplification process (e.g., PCR) of the genome received in the amplification module 600 is performed.

[0116] 1 and 2, the amplification module 600 engages with the outer chamber 100 in a vertical direction. In other words, the upper portion 631 of the housing portion 630 of the amplification module 600 engages with the outer chamber 100 so that it is higher than the lower portion 632 of the housing portion 630 on the ground.

[0117] 17 to 25, the amplification module 600 includes a body 610, inlets 621 and 622, a container 630, a gas transfer passage 640, and an extract transfer passage 650.

[0118] The body 610 is a part that forms the outer shape of the amplification module 600 , and one side of the body 610 is formed with inlet ports 621 and 622 that engage with the outlet ports 128 and 129 of the outer chamber 100 .

[0119] The inlets 621 and 622 are engaged with the discharge holes 128 and 129 and serve as inlets for introducing the extract discharged from the discharge holes 128 and 129 into the storage section 630 .

[0120] Although the amplification module 600 according to the embodiment of the present invention may have two inlets 621 and 622, it is not particularly limited thereto, and embodiments having more than two inlets may also be included in the scope of the present invention.

[0121] In the following, it is assumed that the amplification module 600 according to the embodiment of the present invention has two injection ports 621 and 622.

[0122] One of the two inlets 621, 622, inlet 621, is connected to air flow path 409, and the other inlet 622 is connected to liquid flow path 408. That is, an extract containing a pretreated specimen flows in through the other inlet 622, and in the process, air in storage section 630 is discharged into air flow path 409 through one of the inlets 621.

[0123] The other side of the body 610 is formed with a receiving portion 630, which is a space for receiving the extract that has flowed in through the inlet 621.

[0124] In one example, the receiving portion 630 may be manufactured in a form that completely penetrates one side of the body 610 and the opposite side, or in another example, it may be manufactured in a form that penetrates only one side and does not penetrate the opposite side. Both of these embodiments are the same in that the open portion is sealed with a sealing member. Therefore, the extract and air are introduced into and discharged from the receiving portion 630 only through the gas transfer passage 640 and the extract transfer passage 650.

[0125] One or more receiving units 630 according to an embodiment of the present invention may be provided in one amplification module 600. Fig. 17 shows an amplification module having one receiving unit, Fig. 20 shows an amplification module having two receiving units, and Fig. 23 shows an amplification module having four receiving units.

[0126] The receiving portion 630 may have a substantially trapezoidal shape, and more specifically, it is preferable that the receiving portion 630 has a trapezoidal shape with rounded edges.

[0127] Here, the trapezoidal shape means that the width narrows as it gets farther away from the gas transfer passage 640 and the extract transfer passage 650. The shape of the receiving portion 630 solves the problem of air bubbles being generated when the extract is injected through the extract transfer passage 650. If air bubbles remain in the receiving portion 630, a detection failure that can occur during the fluorescence detection process after the amplification process can occur. The shape of the receiving portion 630 can solve this problem.

[0128] Primers and probes necessary for genome amplification are stored in the storage unit 630. The amplification module 600 according to an embodiment of the present invention includes one or more storage units 630, and each storage unit 630 may be equipped with different types of primers and probes. This has the advantage of allowing multiple detection processes to be performed simultaneously on genomes extracted from a single sample. For example, one storage unit 630a may be equipped with primers and probes for coronavirus amplification, and another storage unit 630b may be equipped with primers and probes for influenza virus amplification, allowing multiple detection processes to be performed simultaneously in a single amplification module 600.

[0129] The gas transfer passage 640 is formed on one surface 611 of the body 610 and is configured to connect the inlet 621 to the upper portion 631 of the receiving portion 630. Conversely, the extract transfer passage 650 is formed on the opposite surface 612 opposite to the one surface 611 and is configured to connect the inlet 622 to the lower portion 632 of the receiving portion 630.

[0130] The gas transfer passage 640 serves as a passage through which the gas in the container 630 moves. 1000 and at the same time has the characteristics of a closed flow path. Because the storage portion 630 is filled with air before the extract is poured, an appropriate volume of air must be discharged to the outside once the extract is poured. In the present invention, the air inside the storage portion 630 is discharged to the air flow path 409 via the gas transfer path 640 through the inlet 621, thereby solving the problem of air bubbles occurring due to residual air remaining as the pressure inside the storage portion 630 decreases. Like the storage portion 630, the gas transfer path 640 also has curved connection portions without any sharp edges, minimizing the occurrence of air bubbles.

[0131] Since gas is lighter than liquid such as the extract, the gas transfer passage 640 is connected to the end of the upper portion 631 of the container 630 .

[0132] When a plurality of receiving portions 630 are provided, it is preferable that the lengths of the gas transfer passages 640 connected to the respective receiving portions 630 are different from each other.

[0133] In an embodiment having a plurality of receiving portions 630, the extract is injected from the lower receiving portion, and the upper receiving portion is injected with a later time. Therefore, the time it takes for air to be discharged from the corresponding receiving portion 630 varies depending on the position of the receiving portion 630. In other words, the lower the receiving portion, the faster the air is discharged through the gas transfer passage 640.

[0134] In addition, not only the air inside the receptacle 630 but also the extract introduced into the receptacle 630 can be discharged through the gas transfer passage 640. Because the multiple gas transfer passages 640 are connected to each other, the extract discharged through one gas transfer passage 640 may be introduced into another receptacle along another gas transfer passage 640, which may cause a problem of mixing of the extract or the amplified product. To solve this problem, in the present invention, the gas transfer passages 640 connected to the receptacle 630 located at the bottom are formed longer, thereby solving the problem of mixing of the extract or the amplified product.

[0135] The gas transfer passages 640 can be configured to have different lengths as shown in FIG. 21 or as shown in FIG.

[0136] Referring to FIG. 24, the gas transfer passage 640 is formed on one surface 611 of the body 610, and is connected to the gas discharge passage 633 connected to the upper portion 631 of the receiving portion 630. The gas transfer passage 640 includes a communication hole 641 penetrating the body 610, a transfer passage 642, a storage passage 643, and a passage pattern forming portion 644.

[0137] The passage pattern forming portion 644 is configured to form a predetermined passage pattern in the transfer passage 642. Taking FIG. 24 as an example, the passage pattern forming portion 644 may have a semicircular shape, and the semicircular passage pattern forming portion 644 may be combined with the linear transfer passage 642 to form the passage pattern shown in FIG. 24. The passage pattern forming portion 644 may be combined with the transfer passage 642 alternately on the left and right sides of the linear transfer passage 642 to form the passage pattern shown in FIG. 24. Here, "combined" means that the empty space in the transfer passage 642 is filled with the shape of the passage pattern forming portion 644, and the filled space does not allow fluid to pass through.

[0138] That is, the portion of the gas transfer passage 640 combined with the passage pattern forming portion 644 corresponds to the transfer passage 642 , and the portion of the gas transfer passage 640 not combined with the passage pattern forming portion 644 corresponds to the storage passage 643 .

[0139] The length of the gas transfer passage 640 increases proportionally as the number of passage pattern forming units 644 and the number of storage passages 643 increase, and the lower the receiving unit is located, the greater the number of passage pattern forming units 644 and storage passages 643. This prevents the extract or amplification product contained in the receiving unit 630 from being mixed.

[0140] The extract transfer passage 650 is formed on the opposite surface 612 of the body 610 from the surface 611, and is configured to connect the inlet 622 to the lower portion 632 of the receiving portion 630. The extract transfer passage 650 serves as a passage through which the extract pretreated in the genome extraction device 1000 moves.

[0141] The extract transfer passage 650 also prevents the extract or amplification product contained in the storage section 630 from mixing, or allows the same amount of extract to be introduced into each storage section 630. If multiple storage sections 630 are provided, the length of each extract transfer passage 650 may be the same, or if the lengths are different, the thickness of each extract transfer passage 650 may be different.

[0142] In addition, in order to prevent the generation of bubbles during the process of the extract moving through the extract moving passage 650, the extract moving passage 650 has curved connecting portions without sharp edges, thereby minimizing the generation of bubbles.

[0143] 25, the extract transfer passage 650 extends from the inlet 622 and branches at one point, with the lower receiving portions being thicker and the upper receiving portions being thinner from the branching point. The thinner the passage, the faster the extract passes through, so the same amount of extract may be poured into both the upper and lower receiving portions.

[0144] The piston 700 is inserted into the piston insertion portion 108 of the outer chamber 100 and is configured to move up and down to suck in the reagent contained in the outer chamber 100 or to expel the reagent sucked into the outer chamber 100 or the amplification module 600.

[0145] 3 and 12, the piston 700 includes an upper piston 710 and a lower piston 720.

[0146] The upper piston 710 has an open top and a fluid receiving portion 701 formed therein to receive the drawn fluid. A sealing portion 711 is installed inside the upper piston 710. The outer surface of the sealing portion 711 is in close contact with the inner surface of the upper piston 710, preventing fluid from entering or exiting through the space between the outer surface of the sealing portion 711 and the inner surface of the upper piston 710. A drive unit mounting portion 711a is recessed in the center of the sealing portion 711 to engage with a drive unit (not shown) of a diagnostic device. The drive unit (not shown) of a diagnostic device engages with the drive unit mounting portion 711a and raises and lowers the sealing portion 711 inside the upper piston 710 to draw fluid into the fluid receiving portion 701 or discharge the fluid received in the fluid receiving portion 701 to the outside.

[0147] An engagement structure that engages with the lower piston 720 may be formed on the bottom surface of the upper piston 710, and a first hole 712 that is connected to the liquid port of the lower piston 720 and a second hole 713 that is connected to the filter port of the lower piston 720 are formed through the bottom surface of the upper piston 710. The second hole 713 is formed to have a smaller diameter than the filter seating space of the filter port to prevent the support structure and the filter from coming off.

[0148] The lower piston 720 is fixed by engaging with a coupling structure formed on the bottom surface of the upper piston 710 .

[0149] The lower piston 720 may include a disk-shaped body 721, a shaft 722 formed to protrude outward from the center of the body 721, and a liquid port 723 and a filter port 724 arranged at the same distance from the center of the body 721.

[0150] The liquid port 723 is used to suck, mix and discharge the specimen and reagent into the piston 700, and the filter port 724 is used to wash the genome collection filter and to separate the genome from the genome collection filter.

[0151] In addition, a groove recessed toward the center may be formed on the outer periphery of the body 721 of the lower piston 720. This groove serves to remove vacuum that may occur when liquid moves inside the extractor.

[0152] Liquid port 723 and filter port 724 are arranged on the same circumference, spaced apart by a certain angle. For example, filter port 724 and liquid port 723 may be spaced apart by 18 to 36 degrees, and more specifically, the two ports may be spaced apart by 22.5 degrees. When using a step motor that rotates once in 16 steps, the positions of liquid port 723 and filter port 724 can be changed with a single drive.

[0153] The filter port 724 of the lower piston 720 may include a filter seating space 725, and a filter and a support structure may be disposed in the filter seating space 725. Glass fiber filters having various particle sizes are used as the filters for genome capture, and the support structure serves to fix the filters for genome capture.

[0154] The support structure may be made of a porous plastic material having a certain granularity to prevent the filter from coming off when fluid is discharged and to maintain a certain pressure.

[0155] The driving unit 800 is connected to a driving unit (not shown) of the diagnostic equipment and serves as a mediator for rotating the piston 700 at a certain angle.

[0156] The driving part 800 may include an engagement groove formed in the center of one surface to engage with the shaft 722, and a driving groove formed on the other surface to engage with a driving part (not shown) of a diagnostic device.

[0157] The drive unit 800 engages with the piston 700 to position the liquid port 723 and the filter port 724 at the appropriate position of the first discharge hole of the outer chamber 100 so that the various chemical reactions required for the genome extraction step can be performed within a single device.

[0158] The liquid port 723 and the filter port 724 are spaced apart at a certain angle, and the drive unit 800 rotates the ports to positions appropriate for each stage during genome extraction.

[0159] The bead chamber 900 includes a first bead chamber 910, a second bead chamber 920, and a dehumidifying chamber 930, which are separated by a first bead chamber partition 901 and a second bead chamber partition 930. bulkhead 902. The first bead chamber 910 is inserted into the first space 106 of the outer chamber 100, and the second bead chamber 920 is inserted into the first space 107 of the outer chamber 100.

[0160] Similar to the inner chamber 200, the upper opening of the bead chamber 900 is also sealed by a third sealing member S3, which is pierced by third protruding members 316 and 317 formed on the bottom surface of the cover 300 when the cover 300 is engaged with the outer chamber 100. Since the upper opening of the bead chamber 900 is opened by the third protruding members 316 and 317, even if a fluid is subsequently introduced into the first bead chamber 910 and the second bead chamber 920, a corresponding amount of air can be discharged through the pierced portion.

[0161] The bottom opening of the bead chamber 900 is open and not sealed by a separate sealing member. Dry beads, more specifically, freeze-dried beads, are stored in the bead chamber 900, which are susceptible to moisture. In the genome extraction device according to the present invention, the bottom opening of the bead chamber 900, the first space of the outer chamber 100, the flow cover 410, the pad 420, the channels of the base plate 400, and the channels of the amplification module 600 are interconnected, but are closed channels that are not exposed to the outside air, thereby minimizing the inflow of moisture into the bead chamber 900.

[0162] The first bead chamber 910 may store several dry beads (b1) required for genome extraction, and the second bead chamber 920 may store several dry beads (b2) required for genome amplification.

[0163] A first bead holder 911 configured to keep dry beads (b1) inside without being discharged to the outside is installed at the top opening of the first bead chamber 910, and a first dehumidifying unit 912 for dehumidifying the internal space of the first bead chamber 910 is installed in the dehumidifying chamber 930. Here, the dry beads required for genome amplification are provided in the form of, for example, capsules, but are not limited thereto.

[0164] A second bead holder 921 configured to keep the dry beads (b2) inside without being discharged to the outside is installed at the upper opening of the second bead chamber 920, and a second dehumidifying unit 922 that dehumidifies the inside of the second bead chamber 920 is installed above the second bead holder 921. A third sealing member S3 seals the second bead chamber 920 so that it does not communicate with the dehumidifying chamber 930 and the first bead chamber 910, but seals the first bead chamber 910 so that they communicate with each other. This will be described in detail with reference to Figures 26 and 27.

[0165] This effect is achieved by configuring a height difference between the first bead chamber partition wall 901 and the second bead chamber partition wall 902. Referring to Figures 26 and 27, the second bead chamber partition wall 902, which separates the second bead chamber 920 and the dehumidification chamber 930, has a higher height than the first bead chamber partition wall 901, which separates the first bead chamber 910 and the dehumidification chamber 930.

[0166] In other words, the top of the second bead chamber partition 902 extends to the same height as the top of the outer partition that forms the second bead chamber 920, and the top of the first bead chamber partition 901 extends to a lower height than the top of the outer partition that forms the first bead chamber 910.

[0167] Therefore, even if the upper opening of the bead chamber 900 is sealed by the third sealing member S3, the first bead chamber 910 and the dehumidifying chamber 930 can communicate with each other through the space between the first bead chamber partition wall 901 and the third sealing member S3. Therefore, the first bead chamber 910 is dehumidified by the second dehumidifying unit 912 installed inside the dehumidifying chamber 930.

[0168] Lower opening of the first bead chamber 910 914 (i.e., the outlet of the first bead chamber) and the lower opening of the second bead chamber 920 924(i.e., the outlet of the second bead chamber) is a discharge passage that narrows from the bead chamber 900 toward the base plate 400. 913,923 It is formed at the end of the

[0169] discharge passage 913,923 The dry beads may be contained inside the discharge passage. 913,923 A bead holder is installed at the top of the 913,923 This can prevent the beads contained in the nozzle from leaking out.

[0170] discharge passage 913,923 The discharge passage may have a tapered shape that narrows toward the base plate 400. 913,923 Lower opening located at the end of 914,924 The diameter of the opening is smaller than the diameter of the dry bead. 914,924 The bead cannot be discharged to the outside through the bottom opening. 914,924 Discharge through the passage 913,923 The fluid flows into the interior of the nozzle, dissolving the dry beads, and the lower opening is only opened through the fluid. 914,924 The fluid may be discharged to the outside (the fluid reservoir of the piston or the amplification module) through the valve.

[0171] Here, the discharge passage of the first bead chamber 910 in which the dry beads necessary for genome amplification are stored is 913 is the discharge passage of the second bead chamber 920 923 400 and may have a wider diameter than the base plate 400 and narrow towards the base plate 400.

[0172] The first bead chamber 910 is the structure into which the last fluid is introduced before the pre-treated extract is introduced toward the amplification module 600. Accurate detection results can only be obtained if the fluid introduced into the first bead chamber 910 is introduced into the receiving part 630 of the amplification module 600 without remaining in the first bead chamber 910 to the maximum extent possible. 913 The discharge passage of the second bead chamber 920923 By forming the first bead chamber 910 to have a wider diameter than the first bead chamber 910 and narrowing it, the amount of fluid remaining in the first bead chamber 910 is minimized.

[0173] The bead chamber 900 according to the present invention also has first locking protrusions 903 and 904 extending from the bottom surfaces of the outer partition walls of the first bead chamber 910 and the second bead chamber 920. As shown in Figures 28 and 30, the first locking protrusions 903 and 904 may extend toward the base plate 400 and be formed in a structure that protrudes outward.

[0174] The outer chamber 100 that engages with the bead chamber 900 has a second locking protrusion on one side of the outer chamber partition wall that divides the first spaces. 119 When a force is applied to the bead chamber 900 toward the base plate 400, the first locking projections 903 and 904 lock against the second locking projections 904. 119 The first locking projections 903 and 904 are engaged with the second locking projections 905 and 906, respectively, so that the first locking projections 903 and 904 are engaged with the second locking projections 905 and 906. 119 When engaged with the outer chamber 100, the relative position of the bead chamber 900 to the outer chamber 100 is fixed.

[0175] The extraction method according to the embodiment of the present invention will be specifically described below.

[0176] First, (a) the inner chamber engages with the outer chamber through the upper openings of the first spaces of the outer chamber, and the fixing portion of the inner chamber preferably engages with the outer chamber while engaging with the inner chamber engaging portion of the safety clip.

[0177] Next, (b) the cover is engaged with the outer chamber, and (c) the safety clip is removed from the outer chamber.

[0178] Next, (d) the cover is pressurized, causing the first protruding member formed on the bottom surface of the cover to break the first sealing member sealing the upper opening of the inner chamber, and the second protruding members formed on the bottom surfaces of the multiple first spaces of the outer chamber to break the second sealing members sealing the lower opening of the inner chamber, causing the reagent contained in the inner chamber to flow out into the multiple first spaces, and (e) by driving the driving unit, the reagent that has flowed out into the multiple first spaces is sucked into and mixed in the fluid containing section inside the upper piston, and then the mixed reagent is discharged to the amplification module.

[0179] Step (e) is carried out in multiple steps, which will be described in more detail below. First, (e1) a sample to be analyzed is introduced into one of the plurality of first spaces of the outer chamber through a sample introduction hole of the cover.

[0180] Next, (e2) the piston installed in the piston accommodating portion of the outer chamber rotates, and the liquid port of the piston communicates with the first discharge hole formed on the bottom surface of any one of the first spaces into which the sample to be analyzed has been introduced.

[0181] Next, (e3) the contact part installed in the internal space of the piston rises, and the sample to be analyzed accommodated in any one of the first spaces is sucked into the fluid accommodation part in the external chamber.

[0182] Next, (e4) the piston rotates, and the liquid port of the piston communicates with the first discharge hole formed in the bottom surface of the other first space.

[0183] Next, (e5) the contact portion rises, and the first reagent contained in the other first space is sucked into the fluid containing portion inside the outer chamber, thereby mixing the sample to be analyzed and the first reagent in the fluid containing portion.

[0184] Next, (e6) the piston rotates, and the liquid port of the piston communicates with the first discharge hole formed in the bottom surface of another one of the first spaces.

[0185] Next, (e7) the contact portion rises, and the second reagent contained in the other first space is sucked into the fluid containing portion inside the outer chamber, thereby mixing the sample to be analyzed with the first reagent and the second reagent.

[0186] Next, (e8) the piston rotates, and the filter port of the piston communicates with the first discharge hole formed in the bottom surface of the other first space.

[0187] Next, (e9) the contact part descends, and the mixture contained in the fluid containing part passes through the genome collection filter installed in the filter port and is discharged into the other first space.

[0188] Next, (e10) the piston rotates, and the liquid port of the piston communicates with the first discharge hole formed in the bottom surface of the first space in which the first reagent, the second reagent and other reagents are accommodated.

[0189] Next, (e11) the contact portion rises, and the other reagent is sucked into and mixed with the fluid containing portion.

[0190] Next, (e12) the piston rotates, and the filter port of the piston communicates with the first discharge hole formed in the bottom surface of the first space containing another reagent.

[0191] Next, (e13) the contact part descends, and the mixed liquid contained in the fluid containing part passes through the genome collection filter and is discharged into the first space containing other reagents.

[0192] Next, (e14) the piston rotates, and the liquid port of the piston communicates with the first discharge hole formed in the bottom surface of the first space containing the eluent.

[0193] Next, (e15) the contact part rises, and the eluent is sucked into the fluid storage part.

[0194] Next, (e16) the piston rotates, and the filter port of the piston communicates with the second discharge hole formed in the bottom surface of the first space containing the beads necessary for genome amplification.

[0195] Next, (e17) the contact portion descends, and the eluent contained in the fluid containing portion passes through the genome collection filter and is discharged into the first space containing the beads necessary for genome amplification, and the genomes captured on the genome collection filter are separated from the genome collection filter and discharged together into the first space.

[0196] Next, (e18) the piston rotates, and the liquid port of the piston communicates with the second discharge hole formed in the bottom surface of the first space in which the genome is accommodated.

[0197] Next, (e19) the contact part rises, and the extract containing the genome is sucked into the fluid storage part.

[0198] Next, (e20) the piston rotates, bringing the liquid port of the piston into communication with the amplification module.

[0199] Next, (e21) the contact part is lowered, and the extract containing the genome contained in the fluid containing part is discharged into the amplification module.

[0200] Next, (e22) the extract is introduced into the storage section of the amplification module through the extract transfer passage of the amplification module.

[0201] Next, (e23) the air remaining in the storage section is discharged to the outside of the amplification module through the gas transfer passage of the amplification module.

[0202] Next, (e24) the amplification device applies heat of a predetermined temperature or higher to the storage section, and the genome is amplified.

[0203] Next, (e25) the presence or absence of disease infection in the sample to be analyzed is determined based on the fluorescence intensity of the amplified product of the genome.

[0204] While the present invention has been described above with reference to the embodiments shown in the drawings so that those skilled in the art can easily understand and reproduce the present invention, these are merely illustrative examples, and those skilled in the art will recognize that various modifications and equivalent embodiments are possible from the embodiments of the present invention. Therefore, the scope of protection of the present invention should be determined by the claims. [Explanation of symbols]

[0205] S1: First sealing member S2: Second sealing member S3: Third sealing member S4, S5: Sealing material 100: Outer chamber 100a: Upper outer surface 100b: Lower outer surface 101,102,103,104,105,106,107: 1st space 108: Piston insertion part 109: Landing section 111, 112, 113, 114, 115: second protruding member 111a, 112a, 113a, 114a, 115a: Protrusion 111b, 112b, 113b, 114b, 115b: Blade part 119:Second locking protrusion 121,122,123,124,125: 1st discharge hole 126,127,129:Second discharge hole 128: Air exhaust hole 130: Insertion space 131: Stopper 200: Inner chamber 201,202,203,204,205:Second space 210: Upper inner chamber 220: Lower inner chamber 230: Fixed part 300: Cover 301: Cover body 302: Lid 307: First insertion hole 308: Second insertion hole 311, 312, 313, 314, 315: First protruding member 316, 317: Third protruding member 320: Separation member 400: Base plate 400a: Piston drive part insertion hole 401, 402, 403, 404, 405, 406, 407, 408: Liquid flow path 409: Air flow path 410: Flow cover 410a: 1st through hole 410b: 1st engagement protrusion 410c: Melt protrusion 410d: Second engagement protrusion 411a, 412a, 413a, 414a, 415a, 416a, 417a, 418a: First flow cover hole 411b, 412b, 413b, 414b, 415b: Second flow cover hole 416b, 417b, 418b: 3rd flow cover hole 419a, 419b: 4th flow cover hole 420: Pad 420a: 2nd through hole 421a, 422a, 423a, 424a, 425a, 426a, 427a, 428a: First pad hole 421b, 422b, 423b, 424b, 425b: Second pad hole 426b, 427b, 428b: 3rd pad hole 429a, 429b: 4th pad hole 420c: Engagement groove 500: Safety clip 510: Outer chamber engagement part 520: Handle 530: Upper extension 540: Side extension 541: Cover support member 542: Inner chamber engagement part 600: Amplification module 610: Body 611: One side 612: Opposite side 621,622: Inlet 630: Storage unit 631: Upper 632: Lower 633: Gas exhaust passage 640: Gas transfer passage 641:Communication hole 642: Passageway 643: Storage passage 644: Passage pattern forming section 650:Extract liquid transfer passage 700: Piston 701: Fluid storage section 710: Upper piston 711: Close contact area 711a: Drive unit installation section 712: 1st hole 713: 2nd hole 720: Lower piston 721: Torso 722: Shaft 723: Liquid port 724: Filter port 800: Drive unit 900: Bead chamber 901: 1st bead chamber bulkhead 902: Second bead chamber bulkhead 903,904: 1st locking protrusion 910: 1st bead chamber 911: 1st bead holder 912: 1st dehumidification section 913: Discharge passage 914: Lower opening 920: Second bead chamber 921: Second bead holder 922:Second dehumidification section 923: Discharge passage 924: Lower opening 930: Dehumidifying chamber 1000: Genome extraction device

Claims

1. an amplification module adapted to engage with the extraction device and to contain the extract to be extracted from the extraction device, body; an inlet formed on the body, the inlet communicating with a discharge hole of the extractor when the body is engaged with the extractor, through which the extracting liquid is introduced, and an outlet communicating with an air flow path of the extractor, through which air is discharged into the air flow path; a plurality of storage spaces formed in the body for storing the extract; a plurality of gas passages connecting the exhaust port and the plurality of storage units, the plurality of gas passages extending from the exhaust port so as to be connected to the plurality of storage units in one-to-one correspondence; and a plurality of extract transfer passages connecting the inlet and the plurality of storage units, the extract transfer passages being formed to extend from the inlet so as to be connected to the plurality of storage units in one-to-one correspondence; the plurality of receiving sections are configured to be disposed at different heights when the amplification module is engaged with the extraction device; The lower the gas transfer passage connected to the storage portion, the longer its length. Amplification module.

2. 2. The amplification module according to claim 1, wherein a connection position at which the gas transfer passage is connected in the storage section is located above a connection position at which the extract transfer passage is connected in the storage section.

3. The amplification module according to claim 1 , wherein the edges of the housing are rounded.

4. The amplification module according to claim 1 , wherein the gas transfer passage and the extract transfer passage are bent one or more times from the inlet or the outlet to the storage section, and the edges of the bent portions are rounded.

5. The amplification module according to claim 3 , wherein the container has a shape whose width narrows as it moves away from the gas passage and the extract passage.

6. The gas transfer passage is a communication hole formed through the body and communicating with the receiving portion; a transfer passage extending from the communication hole; a storage passageway containing a volume of gas; and a path pattern forming portion provided at one or more points of the moving path and closing the portion of the moving path where the path pattern forming portion is provided; 2. The amplification module of claim 1, wherein the number of the storage passages and the number of points at which the passage pattern forming units are provided increase as the gas transfer passages connected to the receiving units are located at an upper position.

7. The amplification module according to claim 1 , wherein the plurality of extract transfer paths are formed to have different lengths.

8. The amplification module according to claim 7 , wherein the thickness of the extract liquid transfer passages connected to the receiving portion located at the upper side of the extract liquid transfer passages is thinner than that of the extract liquid transfer passages connected to the receiving portion located at the upper side of the extract liquid transfer passages.

9. The amplification module according to claim 1 , wherein the container stores a substance for amplifying a genome contained in an extract.

10. The amplification module according to claim 9 , wherein each of the plurality of containers stores a different substance for amplifying a genome.

Images