Portable microfluidics chip system and droplet preparation method

Abstract

To provide a portable microfluidics chip system.SOLUTION: Provided is a microfluidics chip system for generating droplets. The microfluidics chip system includes: a droplet generating device 20 for generating droplets; a power generating device 50 for supplying the droplet generating device with power for generating droplets; a collection bottle for collecting droplets flowing out from the droplet generating device; a connection device 40 for communicating the droplet generating device, the power generating device, and the collection bottle; and a preparation table 10 for fixing the droplet generating device, the power generating device, and the collection bottle. The present application further relates to a method for preparing droplets.SELECTED DRAWING: Figure 1

Description

[Technical Field]

[0001] The present application relates to the field of single-cell sequencing, and in particular to a portable microfluidics chip system and a method for preparing droplets. [Background technology]

[0002] The field of single-cell genomics has advanced very rapidly in recent years, revealing many important clues to complex biological systems, including the ecological diversity of microbial communities and human cancer.

[0003] Single-cell sequencing has traditionally been performed using microfluidics chip technology, which requires droplet sample preparation via an accompanying single-cell sample preparation platform. While these platforms are effective at preparing droplet samples and can be automated, they are relatively complex, requiring at least three pumps to power the flow and droplet generation, as well as other devices and systems connected to the pumps. These platforms require synchronized operation between the pumps to ensure the flow of the three reagents is synchronized. Failure to do so can affect droplet formation, droplet size uniformity, voltage drops in the droplet generation device, and reduce the predictability, programmability, and reproducibility of the continuous and dispersed phase flow rates and droplet generation frequency.

[0004] Furthermore, conventional single cell sample preparation stands are bulky, inconvenient to carry, and have high environmental requirements. Summary of the Invention

[0005] In view of this, the present application provides a microfluidics chip system that can solve the above problems.

[0006] The present invention also provides a method for preparing droplets that can solve the above problems.

[0007] A microfluidics chip system for generating droplets, the microfluidics chip system including: a droplet generating device for generating droplets; a power generating device for supplying power for generating droplets to the droplet generating device; a collection bottle for collecting droplets flowing out from the droplet generating device; a connection device for communicating the droplet generating device, the power generating device, and the collection bottle; and a preparation stand for fixing the droplet generating device, the power generating device, and the collection bottle.

[0008] The droplet generator further comprises a tip body having a continuous phase inlet and a disperse phase inlet formed therein, the continuous phase and disperse phase entering the tip body through the continuous phase inlet and the disperse phase inlet.

[0009] The microfluidics chip system further includes a continuous phase reservoir pool and a dispersed phase reservoir pool, each of which is fixed on the chip body or formed outside the chip body.

[0010] Furthermore, the preparation stand includes a base and a slot for a droplet generator and a slot for a power generator formed on the base, and the droplet generator and the power generator are sequentially accommodated in the droplet generator slot and the power generator slot.

[0011] Further, the base includes a first portion and a second portion connected to the first portion, the first portion being lower than the second portion, the droplet generator slot being formed in the first portion, and the power generator slot being formed in the second portion.

[0012] Furthermore, the collection bottle is located outside the droplet generation device, and the base further includes a collection bottle slot for receiving and securing the collection bottle.

[0013] Furthermore, the collection bottle is housed and fixed within the tip body, and the tip body is housed and fixed in the droplet generator slot.

[0014] Furthermore, the microfluidics chip system includes at least one vibration device, which is disposed in the droplet generator slot, corresponds to the dispersed phase inlet, and applies vibration to the dispersed phase.

[0015] Furthermore, the microfluidics chip system includes at least one vibration device, which is provided in the chip body, corresponds to the dispersed phase inlet, and applies vibration to the dispersed phase.

[0016] Furthermore, the power generating device includes an operating unit and a first positioning member formed at the end of the operating unit, and the preparation table includes a first positioning groove that accommodates and fixes the first positioning member to hold the operating unit in a predetermined position.

[0017] Furthermore, the power generating device is an injection device.

[0018] Furthermore, the power generating device is a negative pressure generating device that is sealingly connected to the collection bottle via the connecting device.

[0019] Furthermore, the preparation stand further includes a fixed stand and a dust cover that is reversibly connected to the fixed stand and positioned on the droplet generation device side, and the dust cover prevents dust from entering the droplet generation device.

[0020] Furthermore, the power generating device is a positive pressure generating device sealed and connected to the continuous phase inlet and the dispersed phase inlet via the connecting device, respectively.

[0021] A method for preparing droplets using the microfluidics chip system described above includes the steps of measuring predetermined amounts of a continuous phase and a dispersed phase using a pipette gun or a pipette tube, introducing the measured continuous phase into the continuous phase inlet, and introducing the measured dispersed phase into the dispersed phase inlet; and activating a power generating device to create a pressure difference within a collection bottle, a connecting device, and a chip body, which causes the pressure difference to cause the dispersed phase and the continuous phase to merge and flow into the collection bottle as droplets.

[0022] The method further includes the step of applying vibration to the dispersed phase with at least one vibration device before activating the power generating device.

[0023] This application provides a microfluidics chip system with the following features. First, the droplet generator, power generator, and collection bottle are fixed to a preparation table, ensuring the stability of droplets generated by the microfluidics chip system. Second, a single power source is applied to the droplet outlet of the droplet generator, creating a vacuum within the droplet generator and synchronously flowing the cell solution, microbead solution, and oil phase within the droplet generator, resulting in a high flow rate and excellent droplet size uniformity. Third, the injection device is powered by a single power source, and the front and rear positions of the injection device operating unit are controlled based on the ideal gas law P1V1=P2V2. A first positioning groove on the base holds the first positioning member of the injection device in a predetermined position, maintaining a vacuum. This system is low-cost and easy to handle. Fourth, the pressure in the power generator changes according to the injection and flow of reagents, and the reagents are filled into the collection device. The pressure change in the power generator also follows the ideal gas law. 5. The voltage drop of the droplet generator, the flow rates of all continuous and dispersed phases, the droplet size and generation frequency are predictable, programmable, and reproducible. 6. The microfluidics chip system is small in volume and can be self-assembled, so it has low requirements for the operating environment, is easy to carry, and allows for scalable production. [Brief explanation of the drawings]

[0024] [Figure 1] 1 is a perspective schematic diagram of a microfluidics chip system according to a first embodiment of the present application. [Figure 2] FIG. 2 is a schematic diagram of the internal structure of the chip body of the microfluidic chip shown in FIG. [Figure 3] 2 is a schematic diagram of the microfluidic chip, collection bottle, and power generating device in the microfluidic chip system shown in FIG. 1, with the power generating device and chip body (cross-sectional view taken along dashed line III-III in FIG. 2) being cross-sectional views. [Figure 4] This is a schematic diagram of the microfluidics chip shown in Figure 3, in which an oil phase is added to the first liquid reservoir pool, a cell solution is added to the second liquid reservoir pool, and a microbead solution is added to the third liquid reservoir pool. [Figure 5] FIG. 5 is a schematic diagram of the power generating device of the microfluidic chip system shown in FIG. 4 after evacuation. [Figure 6] FIG. 1 is a schematic diagram of a microfluidic chip system with a vibration device according to an embodiment of the present application. [Figure 7] FIG. 1 is a schematic diagram of droplets (without microbeads and cells) generated by the microfluidics chip system provided by the present invention. [Figure 8] 1 is a schematic diagram of droplets (containing microbeads and cells) generated by the microfluidics chip system provided by the present invention. [Figure 9] FIG. 2 is a schematic diagram of the internal structure of a chip body according to a second embodiment of the present application. [Figure 10] FIG. 10 is a schematic diagram of the internal structure of a chip body according to a third embodiment of the present application. [Figure 11] FIG. 10 is a schematic diagram of the connection relationship between a microfluidics chip, a collection bottle, and a power generating device according to a fourth embodiment of the present application, in which the power generating device and the chip body are cross-sectional views. [Figure 12] FIG. 10 is a schematic diagram of the connection relationship between a microfluidics chip, a collection bottle, and a power generating device according to a fifth embodiment of the present application, in which the power generating device and the chip body are cross-sectional views. [Figure 13] FIG. 10 is a schematic diagram of the connection relationship between a microfluidics chip, a collection bottle, and a power generating device according to a sixth embodiment of the present application, in which the power generating device and the chip body are shown in cross section. [Figure 14] FIG. 10 is a schematic diagram of the connection relationship between a microfluidics chip, a collection bottle, and a power generating device according to a seventh embodiment of the present application, in which the power generating device and the chip body are shown in cross section. [Figure 15] FIG. 13 is a schematic diagram of the connection relationship between a microfluidics chip, a collection bottle, and a power generating device according to an eighth embodiment of the present application, in which the power generating device and the chip body are shown in cross section. [Figure 16] FIG. 13 is a schematic diagram of the connection relationship between a microfluidics chip, a collection bottle, and a power generating device according to a ninth embodiment of the present application, in which the power generating device and the chip body are cross-sectional views. [Figure 17] FIG. 13 is a schematic diagram of the connection relationship between a microfluidics chip, a collection bottle, and a power generating device according to a tenth embodiment of the present application, in which the power generating device and the chip body are cross-sectional views. [Figure 18] FIG. 20 is a schematic perspective view of a preparation table according to an eleventh embodiment of the present application. [Figure 19] 19 is a perspective schematic view of the preparation table shown in FIG. 18 in another state. [Figure 20] 1 is a flowchart for preparing droplets using a microfluidics chip system without a vibration device. [Figure 21] 1 is a flowchart for preparing droplets using a microfluidics chip system with a vibration device. DETAILED DESCRIPTION OF THE INVENTION

[0025] The present application is further explained in the following detailed description in conjunction with the above-mentioned drawings.

[0026] In order to further describe the technical means and effects that the present application can take to achieve the specified application objectives, the specific embodiments, structures, features, and effects of the microfluidics chip system and droplet preparation method provided by the present application will be described in detail below in conjunction with Figures 1 to 21 and preferred embodiments.

[0027] 1 to 6, a first embodiment of the present application provides a microfluidics chip system 100 for preparing droplets 200.

[0028] 1 , a microfluidics chip system 100 includes a preparation stage 10, a droplet generation device 20, a collection bottle 30, a connection device 40, and a power generation device 50. The preparation stage 10 is used to fix the droplet generation device 20, the collection bottle 30, the connection device 40, and the power generation device 50, the droplet generation device 20 is used to generate droplets 200, the collection bottle is used to collect the droplets 200 flowing out from the droplet generation device 20, and the connection device is used to connect the droplet generation device 20 and the collection bottle 30 and / or the droplet generation device 20 and the power generation device 50 and / or the power generation device 50 and the collection bottle 30. Here, "fixed" includes cases where the droplet generation device 20, the collection bottle 30, and the power generation device 50 cannot be removed from the preparation stage 10, and cases where the droplet generation device 20, the collection bottle 30, and the power generation device 50 can be removed from the preparation stage 10. In this embodiment, the droplet generator 20 , the collection bottle 30 , and the power generator 50 are removable from the preparation stage 10 .

[0029] In this embodiment, the droplet generation device 20, the collection bottle 30, and the power generator 50 are all fixed to the preparation stand 10. The connection device 40 connects the droplet generation device 20 to the collection bottle 30, and connects the collection bottle 30 to the power generator 50. Specifically, the preparation stand 10 includes a base 11 and a droplet generation device slot 12, a collection bottle slot 13, and a power generator slot 14, which are opened in the base 11. The droplet generation device 20, the collection bottle 30, and the power generator 50 are accommodated and fixed in the droplet generation device slot 12, the collection bottle slot 13, and the power generator slot 14, in that order.

[0030] In other embodiments, the droplet generation device 20, the collection bottle 30, and the power generation device 50 may be installed or attached to the base 11 in other ways, for example, the base 11 may be provided with a snap fit or shack device, and the droplet generation device 20, the collection bottle 30, and the power generation device 50 may be fixed to the base 11 by the snap fit or shack, etc.

[0031] Specifically, base 11 includes first surface 111, second surface 112 opposite first surface 111, first side surface 113 connecting first surface 111 and second surface 112, and second side surface 114 opposite first side surface 113. Droplet generator slot 12 and power generator slot 14 are both recessed from first surface 111 and first side surface 113 to second surface 112 and second side surface 114, respectively, and collection bottle slot 13 is recessed from first surface 111 to second surface 112. Specifically, droplet generator slot 12 and power generator slot 14 both penetrate first surface 111 and first side surface 113, and collection bottle slot 13 penetrates only first surface 111.

[0032] In other embodiments, both the droplet generator slot 12 and the power generator slot 14 extend through the first surface 111 only.

[0033] The preparation table 10 can be manufactured by, but is not limited to, a 3D printing method. In this embodiment, the preparation table 10 is manufactured by a 3D printing method, which has low cost and can be adjusted in size according to actual needs.

[0034] In this embodiment, the power generator slot 14 has a first housing portion 141 and a second housing portion 142. Here, the first housing portion 141 houses the main body portion 51 (see FIG. 1 and the following description) of the power generator 50, and the second housing portion 142 houses the operating portion 52 (see FIG. 1 and the following description) of the power generator 50.

[0035] The preparation table 10 further includes a first positioning groove 15 and a second positioning groove 18. The first positioning groove 15 is for receiving and fixing a first positioning member 54 (see FIG. 1 and the following description) of the power generating device 50, and holds the operating part 52 in a predetermined position. The second positioning groove 18 is in communication with the power generating device slot 14, and for receiving and fixing a second positioning member 55 of the power generating device 50 (see FIG. 1 and the following description).

[0036] In this embodiment, the first positioning groove 15 is a recessed groove recessed from the first surface 111 and the first side surface 113 toward the second surface 112 and the second side surface 114, respectively. In other embodiments, the first side surface 113 may function as a stopper of the first positioning groove 15, or the first positioning groove 15 may be a recessed groove that penetrates only the first surface 111 and does not penetrate the first side surface 113.

[0037] Here, the droplet generating device 20 includes a chip body 21. A continuous phase inlet and a dispersed phase inlet are opened in the chip body 21, and a continuous phase introduction passage and a dispersed phase introduction passage are opened inside the chip body 21. The continuous phase inlet is connected to the continuous phase introduction passage, and the dispersed phase inlet is connected to the dispersed phase introduction passage, and the continuous phase introduction passage and the dispersed phase introduction passage intersect and communicate with one end of a confluence passage. The continuous phase enters the continuous phase introduction passage from the continuous phase inlet, and the dispersed phase enters the dispersed phase introduction passage from the dispersed phase inlet, and the continuous phase and dispersed phase merge at the intersection of the continuous phase introduction passage and the dispersed phase introduction passage to enter the confluence passage.

[0038] Here, the continuous phase may be an oil phase or the like, and the dispersed phase may be a cell fluid, a microbead solution, water, etc. In this embodiment, the continuous phase is an oil phase, and the dispersed phase is a cell fluid and a microbead solution.

[0039] Specifically, in this embodiment, chip body 21 includes a third surface 211 and a fourth surface 212 facing away from third surface 211. Oil phase inlet 213 (continuous phase inlet), cell liquid inlet 214 (dispersed phase inlet), microbead solution inlet 215 (dispersed phase inlet), and droplet outlet 216 are each recessed from third surface 211 toward fourth surface 212. Oil phase inlet 213, cell liquid inlet 214, microbead solution inlet 215, and droplet outlet 216 penetrate only third surface 211. In other embodiments, droplet outlet 216 may be provided on one side surface connecting third surface 211 and fourth surface 212.

[0040] An oil phase introduction passage 217 (continuous phase introduction passage), a cell liquid introduction passage 218 (dispersed phase introduction passage), and a microbead solution introduction passage 219 (dispersed phase introduction passage) are also formed within chip body 21. Oil phase inlet 213 communicates with droplet outlet 216 via oil phase introduction passage 217, cell liquid inlet 214 communicates with droplet outlet 216 via cell liquid introduction passage 218, and microbead solution inlet 215 communicates with droplet outlet 216 via microbead solution introduction passage 219.

[0041] In addition, a confluence passage 210 is further formed within the chip body 21, and the oil phase introduction passage 217, the cell liquid introduction passage 218, and the microbead solution introduction passage 219 converge at one end of the confluence passage 210, and the other end of the confluence passage 210 is sealed and connected to the droplet outlet 216.

[0042] In this embodiment, two oil phase introduction passages 217, two cell liquid introduction passages 218, and one microbead solution introduction passage 219 are further formed within chip body 21. Specifically, the two oil phase introduction passages 217 are arranged to surround cell liquid inlet 214 and the two cell liquid introduction passages 218, and the two cell liquid introduction passages 218 are arranged to surround microbead solution inlet 215 and microbead solution introduction passage 219. Before oil phase introduction passage 217, cell liquid introduction passage 218, and microbead solution introduction passage 219 join together, the two oil phase introduction passages 217 and the two cell liquid introduction passages 218 are distributed symmetrically on both sides of microbead solution introduction passage 219.

[0043] In this embodiment, the oil phase inlet 213, the cell solution inlet 214, and the microbead solution inlet 215 are distributed on the same straight line.

[0044] In this embodiment, the oil phase inlet 213, the cell solution inlet 214, the microbead solution inlet 215, and the droplet outlet 216 are not distributed on the same line.

[0045] The microfluidics chip system 100 may further include a continuous phase liquid reservoir pool communicating with the continuous phase inlet and a dispersed phase liquid reservoir pool communicating with the dispersed phase inlet. The continuous phase liquid reservoir pool and the dispersed phase liquid reservoir pool may be located on the chip body 21 or outside the chip body 21.

[0046] 3, in this embodiment, the microfluidics chip system 100 includes a first liquid reservoir pool 22 (continuous phase liquid reservoir), a second liquid reservoir pool 23 (dispersed phase liquid reservoir), and a third liquid reservoir pool 24 (dispersed phase liquid reservoir). The first liquid reservoir pool 22 communicates with an oil phase inlet 213, the second liquid reservoir pool 23 communicates with a cell liquid inlet 214, and the third liquid reservoir pool 24 communicates with a microbead solution inlet 215.

[0047] In this embodiment, the first liquid reservoir pool 22, the second liquid reservoir pool 23, and the third liquid reservoir pool 24 are located on the chip body 21. Specifically, the chip body 21 is integrally formed with the first liquid reservoir pool 22, the second liquid reservoir pool 23, and the third liquid reservoir pool 24. In another embodiment, the first liquid reservoir 22, the second liquid reservoir 23, and the third liquid reservoir 24 may be directly inserted into the oil phase inlet 213, the cell liquid inlet 214, and the microbead solution inlet 215.

[0048] In this embodiment, the first liquid reservoir pool 22 is for temporarily storing the oil phase 80. The storage volume of the first liquid reservoir pool 22 may be, but is not limited to, 100 microliters, 200 microliters, 500 microliters, and 1 milliliter.

[0049] In this embodiment, the second liquid reservoir pool 23 is for temporarily storing the cell liquid 60. The storage volume of the second liquid reservoir pool 23 may be, but is not limited to, 100 microliters, 200 microliters, 500 microliters, and 1 milliliter.

[0050] In this embodiment, the third liquid reservoir pool 24 is for temporarily storing the microbead solution 70. The storage volume of the third liquid reservoir pool 24 may be, but is not limited to, 100 microliters, 200 microliters, 500 microliters, and 1 milliliter. The microbead solution 70 may be any of a polystyrene microbead solution, a resin microbead solution, a magnetic bead solution, or the like.

[0051] In addition, the first liquid reservoir pool 22, the second liquid reservoir pool 23, and the third liquid reservoir pool 24 all have openings (not shown) formed therein to allow the oil phase 80, the cell liquid 60, and the microbead solution 70 to be transferred into the first liquid reservoir pool 22, the second liquid reservoir pool 23, and the third liquid reservoir pool 24.

[0052] The volume ratio of the oil phase 80, the cell solution 60, and the microbead solution 70 in the first liquid reservoir pool 22, the second liquid reservoir pool 23, and the third liquid reservoir pool 24 can be, but is not limited to, 2:1:1. In this embodiment, the cell solution 60 is 200 microliters, the microbead solution 70 is 200 microliters, and the oil phase 80 is 400 microliters.

[0053] In other embodiments, the droplet generating device 20 may not include the first liquid reservoir pool 22, the second liquid reservoir pool 23, and the third liquid reservoir pool 24. In this case, the oil phase 80 may be directly introduced into the oil phase inlet 213, the cell liquid 60 may be directly introduced into the cell liquid inlet 214, and the microbead solution 70 may be directly introduced into the microbead solution inlet 215.

[0054] The collection bottle 30 is for collecting the droplets 200. The collection bottle 30 includes a cover 31 having two through holes (not shown) formed therein. The cover 31 is either detachably connected to the body of the collection bottle 30 or inseparably connected thereto.

[0055] The volume of the collection bottle 30 may be, but is not limited to, 1 milliliter or 5 milliliters. In this embodiment, the volume of the collection bottle 30 is 1 milliliter.

[0056] In this embodiment, the connection device 40 includes a first connection pipe 41 and a second connection pipe 42. The first connection pipe 41 has one end communicating with the power generating device 50 and the other end communicating with the collection bottle 30, and the second connection pipe 42 has one end connected to the chip body 21 and communicating with the droplet outlet 216 and the other end communicating with the collection bottle 30.

[0057] Specifically, in this embodiment, one end of the second connecting pipe 42 is connected to the third surface 211, and the other end is connected to one of the through-holes in the cover 31 of the collection bottle 30, thereby communicating with the collection bottle 30. The first connecting pipe 41 has one end connected to the power generating device 50, and the other end connected to the other through-hole in the cover 31, thereby communicating with the collection bottle 30.

[0058] In this embodiment, the first connecting pipe 41 and the second connecting pipe 42 are both hoses having a length of 10 centimeters.

[0059] In addition, the power generating device 50 supplies power to the droplet generating device 20 to generate droplets 200, and the oil phase 80, cell liquid 60, and bead solution 70 in the first liquid reservoir pool 22, the second liquid reservoir pool 23, and the third liquid reservoir pool 24 flow through the oil phase introduction passage 217, the cell liquid introduction passage 218, and the microbead solution introduction passage 219 of the droplet generating device 20, respectively, and then merge in the confluence passage 210 to form droplets 200 that flow out of the droplet outlet 216 and further into the collection bottle 30.

[0060] In this embodiment, the power generating device 50 is a negative pressure generating device. The power generating device 50 includes a main body 51, an operating unit 52, and a gas inlet / outlet end 53. The main body 51 includes a storage space 511. One end of the operating unit 52 is housed within the storage space 511, and the other end protrudes outside the main body 51. The end of the operating unit 52 housed within the storage space 511 is in close contact with the inner wall of the storage space 511. The end of the operating unit 52 located outside the main body 51 operates the operating unit 52 when an external force is applied, causing the operating unit 52 to slide within the storage space 511. The gas inlet / outlet end 53 is fixed to an end of the main body 51 away from the exposed operating unit 52 and communicates with the storage space 511. The second connecting pipe 42 has one end far from the collection bottle 30 connected to the gas inlet / outlet end 53.

[0061] Specifically, in this embodiment, the power generating device 50 is an injection device.

[0062] In this embodiment, the power generating device 50 further includes a first positioning member 54 and a second positioning member 55 provided at one end of the operating unit 52 exposed to the main body 51. The first positioning member 54 engages with the first positioning groove 15 and pulls the operating unit 52 to a certain position, thereby maintaining a vacuum inside the power generating device 50. The second positioning member 55 is fixed to the main body 51, and by engaging the second positioning member 55 with the second positioning groove 18, restricts movement of the main body 51 in the process of pulling the operating unit 52.

[0063] When the operating part 52 is moved by an external force, and the first positioning member 54 thereon is moved and moved into the first positioning groove 15 to regulate its position, the second connecting pipe 42, the collection bottle 30, the first connecting pipe 41, and the droplet outlet 216, the junction passage 210, the microbead solution introduction passage 219, the cell liquid introduction passage 218, and the oil phase introduction passage 217 of the chip body 21 are expanded from the initial volume V1 to a volume V2, and the ideal gas state equation P1V1=P2V2 (P1 and P2 are the initial volumes V1 and V2) is satisfied. At this time, based on the pressures at the time of the second connecting pipe 42, the collection bottle 30, the first connecting pipe 41, and the droplet outlet 216 of the chip body 21, the confluence passage 210, the microbead solution introduction passage 219, the cell solution introduction passage 218, the oil phase introduction passage 217, the first liquid reservoir pool 22, the second liquid reservoir pool 23, and the third liquid reservoir pool 24, become smaller than atmospheric pressure, and the resulting pressure difference causes the dispersed phase and continuous phase in the droplet generating device 20 to flow into the collection bottle 30 in the form of droplets.

[0064] The power of the power generator 50 can be generated in the following ways: 1. Generate power in advance in a sealed container before connecting to the chip body 21. 2. Drive the power generator 50 with a pump. 3. Generate power by pulling or pushing the plunger of a syringe (or similar device).

[0065] In some embodiments, the microfluidics chip system 100 further includes at least one vibration device, which is provided corresponding to the third liquid reservoir pool and / or the second liquid reservoir pool and applies vibration to the microbead solution 70 and / or the cell solution 60.

[0066] Also, referring to FIG. 6, the microfluidics chip system 100 further includes at least one vibration device, which applies vibration to the dispersed phase.

[0067] Specifically, in this embodiment, the microfluidics chip system 100 further includes a first vibration device 25 and a second vibration device 26. The first vibration device 25 and the second vibration device 26 are fixed in the droplet generator slot 12 and correspond to the cell solution inlet 214 and the microbead solution inlet 215, respectively.

[0068] Furthermore, both the first vibrating device 25 and the second vibrating device 26 may be ceramic vibrating chips.

[0069] In addition, the first vibration device 25 and the second vibration device 26 generate vibrations after being started, which applies vibrations to the cell solution 60 and the microbead solution 70, effectively alleviating the problem of cell and microbead sedimentation that occurs during the experimental process of the cell solution 60 and the microbead solution 70, and ensuring the consistency of the concentrations of the cell solution 60 and the microbead solution 70.

[0070] In another embodiment, the first vibration device 25 and the second vibration device 26 may be located on the chip body 21. For example, the first vibration device 25 and the second vibration device 26 are located on the fourth surface 212 of the chip body 21 and correspond to the cell solution inlet 214 and the microbead solution inlet 215, respectively, or are located on the side wall connecting the third surface 211 and the fourth surface 212 of the chip body 21 and correspond to the cell solution inlet 214 and the microbead solution inlet 215. To alleviate the problem of sedimentation of cells and microbeads in the cell solution 60 and the microbead solution 70, the vibrations generated after the activation of the first vibration device 25 and the second vibration device 26 need only be applied to the cell solution 60 and the bead solution 70.

[0071] In other embodiments, the microfluidics chip system 100 may include only one vibration device, for example, only the second vibration device 26, which is fixed in the droplet generator slot 12 and corresponds to the bead solution inlet 215. The second vibration device 26 may also be fixed to the fourth surface 212 of the chip body 21 and corresponds to the microbead solution inlet 215. This can effectively alleviate the problem of microbead sedimentation in the microbead solution 70 during the experiment and ensure the consistency of the concentration of the microbead solution 70.

[0072] In yet another embodiment, the microfluidics chip system 100 includes only one vibration device, which is provided corresponding to the cell solution inlet 214 and the microbead solution inlet 215 at the same time, and simultaneously applies vibration to the cell solution 60 and the microbead solution 70.

[0073] When power is applied to the power generator 50, a vacuum is created inside the power generator 50, causing the dispersed and continuous phases to flow within the chip body 10. By using a syringe as the power generator, the vacuum can be created with a simple operation. When the reagents (dispersed and continuous phases) begin to flow within the chip body, the air volume within the power generator is expanded or compressed by the positive or negative pressure system, causing a voltage drop at the chip inlets (continuous phase inlet and dispersed phase inlet) and outlet (droplet outlet). This gradually slows the reagent flow rate, and the change in reagent flow rate causes a change in droplet size. This makes the voltage drop within the chip body 10, the flow rates of the dispersed and continuous phases, and the droplet size and generation frequency predictable, programmable, and reproducible.

[0074] Referring to Figure 7, Figure 7 is a schematic diagram of droplets (not including microbeads or cells) generated by the microfluidic chip system provided by the present invention. As can be seen from Figure 7, the droplets generated by the microfluidic chip system 100 provided by the present invention have small variations in droplet size and good uniformity. In this embodiment, the droplet size is 55.7 μm, and the droplet size variation is 2%.

[0075] 8, which is a schematic diagram of droplets (containing microbeads and cells) generated by the microfluidics chip system 100 provided by the present invention. As can be seen from FIG. 8, the microfluidics chip system provided by the present invention can encapsulate microbeads and cells in an oil phase.

[0076] 9 is a schematic diagram of the internal structure of a chip body 110 according to a second embodiment of the present application. The internal structure of the chip body 110 is substantially the same as the internal structure of the chip body 21 according to the first embodiment of the present application, except that two oil phase introduction passages 217, one cellular liquid introduction passage 218, and two microbead solution introduction passages 219 are formed within the chip body 110. Specifically, the two oil phase introduction passages 217 are arranged to surround the microbead solution inlet 215 and the two microbead solution introduction passages 219, and the two microbead solution introduction passages 219 are arranged to surround the cellular liquid inlet 214 and the one cellular liquid introduction passage 218. Before the oil phase introduction passage 217, the cellular liquid introduction passage 218, and the microbead solution introduction passage 219 join together, the two oil phase introduction passages 217 and the two microbead solution introduction passages are symmetrically distributed on both sides of the microbead solution introduction passage 219.

[0077] In this embodiment, the oil phase inlet 213, the cell solution inlet 214, the microbead solution inlet 215, and the droplet outlet 216 are distributed on the same line.

[0078] Referring to Figure 10, Figure 10 is a schematic diagram of the internal structure of a chip body 120 according to the third embodiment of the present application. The internal structure of chip body 120 is substantially the same as the internal structure of chip body 21 according to the first embodiment of the present application, except that two oil phase introduction passages 217, one cellular liquid introduction passage 218, and one microbead solution introduction passage 219 are formed within chip body 120. Specifically, the two oil phase introduction passages 217 are arranged to surround microbead solution inlet 215 and one microbead solution introduction passage 219, and cellular liquid inlet 214 and one cellular liquid introduction passage 218. The one cellular liquid introduction passage 218 and the one microbead solution introduction passage 219 are distributed symmetrically, and the two oil phase introduction passages 217 are distributed on both sides of the one cellular liquid introduction passage 218 and one microbead solution introduction passage 219.

[0079] In this embodiment, the oil phase inlet 213 and the droplet outlet 216 are distributed on the same line, and the oil phase inlet 213, the cell solution inlet 214, the microbead solution inlet 215 and the droplet outlet 216 are not distributed on the same line.

[0080] 11 is a schematic diagram of the connection relationship between the microfluidics chip body 21, collection bottles 30, and power generator 50 according to a fourth embodiment of the present application, with the power generator 50 being a cross-sectional view. In this embodiment, the connection relationship between the chip body 21, collection bottles 30, and power generator 50 is substantially the same as the connection relationship between the chip body 21, collection bottles 30, and power generator 50 shown in FIG. 3, except that the first liquid reservoir pool 22, the second liquid reservoir pool 23, and the third liquid reservoir pool 24 are formed outside the chip body 21 and are sealed and connected to the oil phase inlet 213, the cell liquid inlet 214, and the microbead solution inlet 215 by the third connecting pipe 43, the fourth connecting pipe 44, and the fifth connecting pipe 45, respectively.

[0081] 12, which is a schematic diagram of the connection relationship between the microfluidics chip body 21, the collection bottle 30, and the power generating device 50 according to the fifth embodiment of the present application, with the power generating device 50 being a cross-sectional view. In this embodiment, the connection relationship between the chip body 21, the collection bottle 30, and the power generating device 50 is basically the same as the connection relationship between the chip body 21, the collection bottle 30, and the power generating device 50 shown in FIG. 9, except that the droplet generating device 20 further includes a protrusion 27 formed on the chip body 21, the collection bottle 30 is placed within the protrusion 27, the junction passage 210 and the collection bottle 30 are in sealed communication, and the collection bottle 30 is in sealed communication with the power generating device 50 via a first connecting pipe 41.

[0082] 13 is a schematic diagram of the connection relationship between the microfluidics chip body 21, the collection bottles 30, and the power generator 50 according to the sixth embodiment of the present application, with the power generator 50 being shown in cross section. In this embodiment, the connection relationship between the chip body 21, the collection bottles 30, and the power generator 50 is substantially the same as the connection relationship between the chip body 21, the collection bottles 30, and the power generator 50 shown in FIG. 12 , except that the first liquid reservoir pool 22, the second liquid reservoir pool 23, and the third liquid reservoir pool 24 are formed on the chip body 21, the first liquid reservoir pool 22 is in sealing communication with the oil phase inlet 213, the second liquid reservoir pool 23 is in sealing communication with the cell liquid inlet 214, and the third liquid reservoir pool 24 is in sealing communication with the microbead solution inlet 215.

[0083] Referring to FIG. 14 , FIG. 14 is a schematic diagram of the connection relationship between the microfluidics chip body 21, the collection bottle 30, and the power generator 50 according to the seventh embodiment of the present application, with the power generator 50 being a cross-sectional view. In this embodiment, the connection relationship between the chip body 21, the collection bottle 30, and the power generator 50 is substantially the same as the connection relationship between the chip body 21, the collection bottle 30, and the power generator 50 shown in FIG. 3 , except that the power generator 50 is a positive pressure generator. Specifically, the power generator 50 is an injection device. The power generator 50 is connected to each of the first liquid reservoir pool 22, the second liquid reservoir pool 23, and the third liquid reservoir pool 24 via a first connecting pipe 41. In this embodiment, the first connecting pipe 41 is a branched connecting pipe with multiple terminals. Specifically, the first connection pipe 41 includes a main connection segment 411, a first branch connection segment 412, a second branch connection segment 413, and a third branch connection segment 414, and one end of the main connection segment 411 is connected to and sealed with the gas inlet / outlet end 53 of the power generating device 50, and the other end of the main connection segment 411 is connected to and sealed with the first branch connection segment 412, the second branch connection segment 413, and the third branch connection segment 414, respectively.

[0084] Of course, the structure of this embodiment can also be applied to the structures of the chip body shown in FIGS.

[0085] 15 is a schematic diagram of the connection relationship between the microfluidics chip body 21, collection bottles 30, and power generator 50 according to the eighth embodiment of the present application, with the power generator 50 being a cross-sectional view. In this embodiment, the connection relationship between the chip body 21, collection bottles 30, and power generator 50 is substantially the same as the connection relationship between the chip body 21, collection bottles 30, and power generator 50 shown in FIG. 14, except that the first liquid reservoir pool 22, the second liquid reservoir pool 23, and the third liquid reservoir pool 24 are formed outside the chip body 21 and are sealed and connected to the oil phase inlet 213, the cell liquid inlet 214, and the microbead solution inlet 215 by the third connecting pipe 43, the fourth connecting pipe 44, and the fifth connecting pipe 45, respectively.

[0086] 16, which is a schematic diagram of the connection relationship between the microfluidics chip body 21, the collection bottle 30, and the power generating device 50 according to the ninth embodiment of the present application, with the power generating device 50 being a cross-sectional view. In this embodiment, the connection relationship between the chip body 21, the collection bottle 30, and the power generating device 50 is basically the same as the connection relationship between the chip body 21, the collection bottle 30, and the power generating device 50 shown in FIG. 15, except that the droplet generation device 20 further includes a protrusion 27 formed on the chip body 21, the collection bottle 30 is placed within the protrusion 27, and the junction passage 210 is sealed and connected to the collection bottle 30.

[0087] 17 is a schematic diagram of the connection relationship between the microfluidics chip body 21, collection bottles 30, and power generator 50 according to the tenth embodiment of the present application, with the power generator 50 being shown in cross section. In this embodiment, the connection relationship between the chip body 21, collection bottles 30, and power generator 50 is basically the same as the connection relationship between the chip body 21, collection bottles 30, and power generator 50 shown in FIG. 16, except that the first liquid reservoir pool 22, the second liquid reservoir pool 23, and the third liquid reservoir pool 24 are formed on the chip body 21, the first liquid reservoir pool 22 is in sealing communication with the oil phase inlet 213, the second liquid reservoir pool 23 is in sealing communication with the cell liquid inlet 214, and the third liquid reservoir pool 24 is in sealing communication with the microbead solution inlet 215.

[0088] 18-19, which are perspective schematic diagrams of a preparation stage according to an eleventh embodiment of the present application. In this embodiment, the structure of the preparation stage 101 is similar to the structure of the preparation stage 10 in the first embodiment. The difference is that when the pressure generator is a negative pressure generator, the base 11 of the preparation stage 101 includes a first portion 115 and a second portion 116 connected to the first portion 115. The first portion 115 is lower than the second portion 116, the droplet generator slot 12 is formed in the first portion 115, and the power generator slot 14 is formed on the second portion 116. In other embodiments, the droplet generator slot 12 may be omitted, and the droplet generator 20 may be directly fixed to the first portion 115. In this embodiment, the collection bottle slot 13 is formed on the second portion 116. Of course, if the collection bottle 30 is formed in the chip body 21, the second part 116 will not have the collection bottle slot 13. The preparation stand 101 further includes a fixed base 16 fixed to the first part 115 and a dust cover 17 reversibly connected to the fixed base 16. The dust cover 17 is located on the chip body 21 side and prevents dust from entering the chip body 21. Specifically, the dust cover 17 is located above the continuous phase inlet and / or the dispersed phase inlet, or above the continuous phase liquid reservoir pool and / or the dispersed phase liquid reservoir pool.

[0089] Referring to Figures 20-21, a preferred embodiment of the present application provides a preparation method for preparing droplets, which is performed in combination with the microfluidics chip system 100 introduced above or a system similar thereto, and includes the following steps:

[0090] Step S801 involves measuring predetermined amounts of the continuous phase and the dispersed phase using a pipette gun or a pipette tube, and introducing the measured continuous phase into the continuous phase inlet and the measured dispersed phase into the dispersed phase inlet.

[0091] Specifically, in this embodiment, step S801 can be expressed as follows: Using a pipette gun or a pipette tube, predetermined amounts of the oil phase 80, the cell liquid 60, and the microbead solution 70 are respectively measured, and the measured oil phase 80 is introduced into the oil phase inlet 213 of the microfluidics chip system 100, the measured cell liquid 60 is introduced into the cell liquid inlet 214, and the measured microbead solution 70 is introduced into the microbead solution inlet 215.

[0092] If the microfluidics chip system 100 further includes a continuous phase reservoir pool and a dispersed phase reservoir pool, step S801 can be expressed as follows: measure predetermined amounts of the continuous phase and dispersed phase, respectively, using a pipette gun or a pipette tube, and introduce the measured continuous phase into the continuous phase reservoir pool, and introduce the measured dispersed phase into the dispersed phase reservoir pool.

[0093] Specifically, in this embodiment, since the microfluidics chip system 100 further includes a first liquid reservoir pool 22, a second liquid reservoir pool 23, and a third liquid reservoir pool 24, step S801 specifically involves using a pipette gun or a pipette tube to measure a predetermined amount of each of the oil phase 80, the cell liquid 60, and the microbead solution 70, introducing the measured oil phase 80 into the first liquid reservoir pool 22, introducing the measured cell liquid 60 into the second liquid reservoir pool 23, introducing the measured microbead solution 70 into the third liquid reservoir pool 24, introducing the oil phase 80 into the oil phase inlet 213 via the first liquid reservoir pool 22, introducing the cell liquid 60 into the cell liquid inlet 214 via the second liquid reservoir pool 23, and introducing the microbead solution 70 into the microbead solution inlet 215 via the third liquid reservoir pool 24.

[0094] In this embodiment, the cell solution 60 is 200 microliters, the microbead solution 70 is 200 microliters, and the oil phase 80 is 400 microliters.

[0095] Furthermore, the loading order of the cell liquid 60, microbead solution 70, and oil phase 80 may be such that the cell liquid 60 is added first, then the microbead solution 70, and finally the oil phase 80; alternatively, the microbead solution 70 is added first, then the cell liquid 60, and finally the oil phase 80; or the cell liquid 60, microbead solution 70, and oil phase 80 may be added simultaneously.

[0096] Step S802 starts the power generating device 50 to create a pressure difference between the pressure in the collection bottle 30, the connection device 40, and the chip body 21 and atmospheric pressure, and the pressure difference causes the dispersed phase and the continuous phase to merge and flow into the collection bottle 30 as droplets.

[0097] Specifically, in step S802, the power generating device 50 is activated to create a pressure difference between the pressure in the collection bottle 30, the second connecting pipe 42, the droplet outlet 216 in the chip body 21, the confluence passage 210, at least one dispersed phase introduction passage, the dispersed phase inlet, and the continuous phase inlet and atmospheric pressure, and the pressure difference causes the continuous phase and dispersed phase to flow into the confluence passage 210, where they merge to form droplets 200, which then flow further into the collection bottle 30 through the droplet outlet 216 and the second connecting pipe 42 due to the pressure difference.

[0098] In this embodiment, step S802 can be expressed as follows: Activating the power generator 50 creates a pressure difference between atmospheric pressure and the pressures in the collection bottle 30, the second connecting pipe 42, and the droplet outlet 216, the confluence passage 210, the microbead solution introduction passage 219, the cell solution introduction passage 218, the oil phase introduction passage 217, the microbead solution inlet 215, the cell solution inlet 214, and the oil phase inlet 213 within the chip body 21. This pressure difference causes the cell solution 60, the microbead solution 70, and the oil phase 80 to flow into the confluence passage 210, forming droplets 200 in the confluence passage 210. The pressure difference then causes the droplets 200 to flow further through the droplet outlet 216 and the second connecting pipe 42 into the collection bottle 30.

[0099] In this embodiment, the droplet generation device 20 of the microfluidics chip system 100 further includes a first liquid reservoir pool 22, a second liquid reservoir pool 23, and a third liquid reservoir pool 24. Therefore, in step S802, the power generating device 50 is activated to create a pressure difference between atmospheric pressure and the pressures in the collection bottle 30, the second connecting pipe 42, and the droplet outlet 216, the confluence passage 210, the microbead solution introduction passage 219, the cell liquid introduction passage 218, the oil phase introduction passage 217, the third liquid reservoir pool 24, the second liquid reservoir pool 23, and the first liquid reservoir pool 22 in the chip body 21. This pressure difference causes the cell liquid 60, the microbead solution 70, and the oil phase 80 to flow into the confluence passage 210, where they merge to form droplets 200. The pressure difference then causes the droplets 200 to flow further through the droplet outlet 216 and the second connecting pipe 42 into the collection bottle 30.

[0100] In this embodiment, the power generator 50 is a negative pressure generator, and therefore, in step S802, the power generator 50 is activated to generate negative pressure in the collection bottle 30, the second connecting tube 42, and the droplet outlet 216, the microbead solution introduction passage 219, the cell solution introduction passage 218, and the oil phase introduction passage 217 in the chip body 21. This negative pressure causes the cell solution 60, the microbead solution 70, and the oil phase 80 to flow into the junction passage 210, forming droplets 200 in the junction passage 210. The pressure difference then causes the droplets 200 to flow further through the droplet outlet 216 and the second connecting tube 42 into the collection bottle 30. Furthermore, the specific operation for generating negative pressure involves pulling the operating part 52 of the power generator 50 to a predetermined position and fixing the first positioning member 54 in the first positioning groove 15 on the base 11.

[0101] In another embodiment, since the power generating device 50 is a positive pressure generating device, in step S802, specifically, the power generating device 50 is activated to generate positive pressure in the dispersed phase introduction passage, the continuous phase introduction passage, the droplet outlet 216, and the collection bottle 30 within the chip body 21, and the positive pressure causes the dispersed phase and the continuous phase to flow into the confluence passage 210, where they merge to form droplets 200, and the positive pressure causes the droplets 200 to flow further into the collection bottle 30 via the droplet outlet 216.

[0102] 19 , in another embodiment, the preparation method may further include step S900 before starting the power generating device 50. In step S900, the first vibration device 25 and the second vibration device 26 apply vibration to the microbead solution 70 and the cellular solution 60. For example, by turning on power switches (not shown) of the first vibration device 25 and the second vibration device 26 provided on the base 11, the first vibration device 25 and the second vibration device 26 start vibration so that vibration is applied to the microbead solution 70 and the cellular solution 60.

[0103] Since the microfluidics chip system 100 may include only one vibration device, in another embodiment, step S900 can be expressed as follows: vibrating the bead solution 70 and / or the cell solution 60 by at least one vibration device.

[0104] In another embodiment, step S900 can be expressed as follows: The first vibration device 25 and the second vibration device 26 apply vibration to the dispersed phase, for example, by turning on power switches (not shown) of the first vibration device 25 and the second vibration device 26 provided on the base 11, the first vibration device 25 and the second vibration device 26 start vibration so as to apply vibration to the dispersed phase.

[0105] This application provides a microfluidics chip system with the following features: 1. The droplet generator, power generator, and collection bottle are fixed to a preparation table, ensuring the stability of droplets generated by the microfluidics chip system. 2. A single power source is applied to the droplet outlet of the droplet generator, creating a vacuum within the droplet generator and synchronously flowing the cell solution, microbead solution, and oil phase within the droplet generator, resulting in a high flow rate and excellent droplet size uniformity. 3. The injection device is further powered by a single power source, and the front and rear positions of the injection device operating unit are controlled based on the ideal gas law P1V1=P2V2. A first positioning groove on the base holds the first positioning member of the injection device in a predetermined position, maintaining a vacuum state. This makes the system low-cost and easy to handle. 4. The pressure in the power generator changes according to the injection and flow of reagents, and the reagents are filled into the collection device. The pressure change in the power generator also follows the ideal gas law. 5. The voltage drop of the droplet generator, the flow rates of all continuous and dispersed phases, the droplet size and generation frequency are predictable, programmable, and reproducible. 6. The vibration device installed in the microfluidics chip system effectively improves the problem of cell and / or microbead sedimentation that occurs during the experimental process of cell solution and / or microbead solution, and can ensure the consistency of the cell solution and / or microbead solution concentration.

[0106] The microfluidics chip system provided by the present application can prepare 250,000 droplets in 2 minutes, resulting in 10,000 single-cell target products, i.e., each droplet contains one magnetic bead and one cell. The droplet generation time can be adjusted to control the amount of reagent target product according to actual needs. The entire volume of the microfluidics chip system is only the size of an Apple iPad mini, allowing users to greatly expand their working space.

[0107] The above-described embodiments are merely preferred embodiments of the present application and are not intended to limit the present application in any way. Although preferred embodiments are disclosed as above, they are not intended to limit the present application. Equivalent embodiments created by some changes and modifications using the technical solutions disclosed above, as long as they do not deviate from the technical solutions of the present application, any simple changes, equivalent changes, and modifications of the above-described embodiments essentially belong to the technical solutions of the present application, as long as they do not deviate from the technical solutions of the present application. [Explanation of symbols]

[0108] 100 Microfluidics Chip System 10,101 Preparation table 11. Base 111 1st surface 112 Second surface 113 First aspect 114 Second aspect 115 Part 1 116 Part 2 12 Droplet generator slot 13 Harvesting Jar Slot 14 Power Generator Slot 141 First Storage Unit 142 Second Storage Unit 15 First positioning groove 18 Second positioning groove 16 Fixed base 17 Dust cover 20 Droplet generator 21,110,120 Chip body 211 Third surface 212 4th surface 213 Oil phase inlet 214 Cell fluid inlet 215 Microbead solution inlet 216 Droplet outlet 217 Oil phase introduction passage 218 Cell fluid introduction passage 219 Microbead solution introduction passage 210 Merging Passage 22 First liquid reservoir pool 23 Second liquid reservoir pool 24 Third liquid reservoir pool 25 First vibration device 26 Second vibration device 30 Collection Bottle 31 Cover 40 Connection Device 41 First connecting pipe 42 Second connecting pipe 50 Power Generator 51 Main body 511 Containment Space 52 Operation section 53 Gas inlet / outlet end 54 First positioning member 55 Second positioning member 60 cell fluid 70 microbead solution 80 Oil phase 200 droplets 43 Third connecting pipe 42 Fourth connecting pipe 45 5th connecting pipe 27 Convex part

Claims

1. A portable microfluidics chip system including: a droplet generating device that generates droplets; a power generating device that supplies power to the droplet generating device to generate droplets; a collection bottle that collects droplets flowing out from the droplet generating device; a connection device that connects the droplet generating device, the power generating device, and the collection bottle; and a preparation stand that fixes the droplet generating device, the power generating device, and the collection bottle, The droplet generator includes a tip body having a continuous phase inlet for receiving a continuous phase, a dispersed phase inlet for receiving a dispersed phase, and a droplet outlet for discharging the droplets; a continuous phase introduction passage, a dispersed phase introduction passage, and a merging passage are formed in the chip body, the continuous phase inlet communicates with the droplet outlet via the continuous phase introduction passage, and the dispersed phase inlet communicates with the droplet outlet via the dispersed phase introduction passage; the continuous phase introduction passage and the dispersed phase introduction passage are joined at one end of the joining passage, the joining passage extends in a serpentine manner, and the other end of the joining passage is sealed and connected to the droplet outlet, the power generating device includes a main body portion including an accommodation space and an operating portion, one end of the operating portion is accommodated in the accommodation space and is in close contact with an inner wall of the accommodation space, and the other end of the operating portion protrudes outside the main body portion; the power generating device includes a first positioning member formed at an end of the operating unit; The preparation table further includes a first positioning groove; The portable microfluidics chip system is characterized in that the operating unit fixes the first positioning member in the first positioning groove by pulling the first positioning member to a preset position by an external force.

2. the power generating device includes a gas inlet / outlet end; The portable microfluidics chip system according to claim 1, characterized in that the gas inlet / outlet end is fixed to one end of the main body part away from the operation part exposed to the outside and communicates with the storage space.

3. the power generating device is a negative pressure generating device, The portable microfluidics chip system according to claim 2 , wherein the gas inlet / outlet end is sealedly connected to the collection bottle via the connecting device.

4. the connecting device includes a first connecting pipe and a second connecting pipe; One end of the first connecting pipe is connected to the gas inlet / outlet end, and the other end of the first connecting pipe is connected to the collecting bottle; 4. The portable microfluidics chip system according to claim 3, wherein one end of the second connecting tube is connected to the chip body and communicates with the droplet outlet, and the other end of the second connecting tube is communicated with the collection bottle.

5. the power generating device is a positive pressure generating device, the connecting device includes a first connecting pipe and a second connecting pipe; 3. The portable microfluidics chip system of claim 2, wherein the gas inlet and outlet ends are sealedly connected to the continuous phase inlet and the dispersed phase inlet through the first connecting pipe, respectively; one end of the second connecting pipe is connected to the chip body and communicates with the droplet outlet; and the other end of the second connecting pipe is communicated with the collection bottle.

6. The portable microfluidics chip system according to claim 1, wherein the continuous phase is an oil phase.

7. 7. The portable microfluidics chip system according to claim 6, wherein the dispersed phase is a cell solution and a microbead solution.

8. 2. The portable microfluidics chip system according to claim 1, wherein the collection bottle is accommodated and fixed within the chip body, and the volume of the collection bottle is 1 milliliter or 5 milliliters.

9. The portable microfluidics chip system according to claim 1, characterized in that the preparation stand further includes a fixed stand and a dust cover reversibly connected to the fixed stand and positioned on the droplet generation device side, the dust cover preventing dust from entering the droplet generation device.

10. The portable microfluidic chip system according to claim 1, further comprising at least one vibration device for applying vibration to the dispersed phase.

11. the portable microfluidic chip system further comprises a continuous phase reservoir pool in communication with the continuous phase inlet, and a dispersed phase reservoir pool in communication with the dispersed phase inlet; 2. The portable microfluidics chip system of claim 1, wherein the metered continuous phase enters the continuous phase inlet by being introduced into the continuous phase liquid reservoir pool, and the metered dispersed phase enters the dispersed phase inlet by being introduced into the dispersed phase liquid reservoir pool.

12. the continuous phase introduction passage is provided along the periphery of the dispersed phase, or The portable microfluidics chip system according to claim 11, wherein the continuous phase inlet and the dispersed phase inlet are distributed on the same line.

13. A method for preparing droplets using the portable microfluidics chip system according to any one of claims 1 to 12, comprising: The method includes the steps of: measuring predetermined amounts of the continuous phase and the dispersed phase using a pipette gun or a pipette tube, introducing the measured continuous phase into a continuous phase inlet, and introducing the measured dispersed phase into a dispersed phase inlet; and activating a power generating device to generate a pressure difference within the collection bottle, the connection device, and the tip body, which causes the dispersed phase and the continuous phase to merge and flow into the collection bottle as droplets.

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