Apparatus, methods, and kits for preparing cell suspensions

Abstract

To provide a kit and a method for preparing a cell suspension.SOLUTION: A kit for preparing a cell suspension may include a device and a housing. The device may include a first label identifying a first reservoir of the device for use with a first portion of a tissue processing method and a second label identifying a second reservoir of the device for use with a second portion of the tissue processing method. The housing includes a first housing portion 430 configured to store a first set of components associated with the first portion of the tissue processing method. The first housing portion includes a first visual indicator 432 associated with the first label of the device. A second housing portion 440 may be configured to store a second set of components 443 associated with the second portion of the tissue processing method, and may include a second visual indicator 442 associated with the second label of the device.SELECTED DRAWING: Figure 10

Description

[Technical Field]

[0001] The present invention generally relates to apparatus, methods, and kits for processing cells, such as preparing cell suspensions. Regarding the field of [unclear / unclear]. [Background technology]

[0002] Applying cell suspensions to the patient's tissue treatment site can be used for wounds (e.g., burns, chronic wounds). ), it may act as an effective treatment for skin diseases such as pigment disorders. However, cell suspension Current methods, which involve preparing a liquid and applying it to the skin surface, require, for example, to complete the work step. Due to the number of similar tools and the total number of components required, user errors and inefficiencies are common. In contrast, it can be vulnerable. Therefore, improvements in cell suspension preparation techniques and equipment are needed. It is being done. [Overview of the project]

[0003] In some embodiments, the kit for preparing the cell suspension includes the apparatus and how The apparatus may include a sing. The apparatus may specify a first container and a second container. The apparatus includes a first label that identifies a first container used in the first part of the tissue processing method, A second label for identifying a second container used in the second part of the tissue processing method, is included. The housing may be configured to receive the device. To store a first set of components associated with a first part of the tissue processing method. It may include a first housing section configured as follows. The first housing section is the first of the apparatus. It may include a first visual indicator associated with label 1. A second how The zing section comprises a second set of components associated with the second part of the tissue processing method. It may be configured to store. The second housing portion is related to the second label of the device. It may also include a second visual indicator that is linked to it.

[0004] In some embodiments, packaging for an apparatus for preparing cell suspensions The stem may comprise a first housing section and a second housing section. The device is Even if it includes a first container having a first label and a second container having a second label Good. The first housing part is associated with the first part of the tissue processing method, a first set It may include a set of recesses configured to receive a component. The zing section includes a first visual indicator associated with a first label of the device. The second housing portion is a second one associated with the second part of the tissue processing method. It may include a set of recesses configured to receive the set of components. The housing portion includes a second visual indicator associated with a second label of the device. That's fine.

[0005] In some embodiments, a device packaged in a housing is used for cell suspension. A method for preparing turbidity involves a first label and a first container of the apparatus, which are positioned close to the first container of the apparatus. First matching with the first visual indicator included in the first housing portion of the wug This may include separating. In response to the identification of the first matching, from the first housing portion Take out the first set of components, and use the first set of components to the first The first part of the tissue processing method associated with the container may be performed. A second label disposed adjacent to the second container and a second visual indicator included in the second housing portion of the housing may be identified. The second Upon identification of the second match, a second set of components may be removed from the second housing portion and the second portion of the tissue processing method associated with the second container may be performed using the second set of components.

[0006] The patent or application file includes drawings created in at least one color. A copy of this patent or patent application publication with color drawings is provided by the Patent Office upon payment of the required fees and claims.

Brief Description of the Drawings

[0007] [Figure 1] It is a schematic block diagram of a kit for preparing a cell suspension according to one embodiment. [Figure 2] It is a schematic diagram of a kit for preparing a cell suspension according to one embodiment. [Figure 3] It is a flowchart of a method for preparing a cell suspension using an apparatus packaged in a housing according to some embodiments. [Figure 4] It is a diagram showing components of a kit for preparing a cell suspension according to one embodiment. [Figure 5] It is a diagram showing components of a kit for preparing a cell suspension according to one embodiment. [Figure 6] It is a diagram showing components of a kit for preparing a cell suspension according to one embodiment. [Figure 7] It is a diagram showing components of a kit for preparing a cell suspension according to one embodiment. [Figure 8] It is a diagram showing components of a kit for preparing a cell suspension according to one embodiment. ​​​ [Figure 9] This figure shows the components of a kit for preparing a cell suspension according to one embodiment. [Figure 10] This figure shows the components of a kit for preparing a cell suspension according to one embodiment. [Figure 11] This figure shows the components of a kit for preparing a cell suspension according to one embodiment. [Figure 12] This figure shows the components of a kit for preparing a cell suspension according to one embodiment. [Figure 13] This figure shows the components of a kit for preparing a cell suspension according to one embodiment. [Figure 14] This figure shows the components of a kit for preparing a cell suspension according to one embodiment. [Figure 15] This figure shows the components of a kit for preparing a cell suspension according to one embodiment. [Figure 16] This figure shows the components of a kit for preparing a cell suspension according to one embodiment. [Figure 17] This figure shows the components of a kit for preparing a cell suspension according to one embodiment. [Figure 18] This figure shows the components of a kit for preparing a cell suspension according to one embodiment. [Figure 19] This figure shows the components of a kit for preparing a cell suspension according to one embodiment. [Figure 20] This figure shows the components of a kit for preparing a cell suspension according to one embodiment. [Figure 21] This figure shows the components of a kit for preparing a cell suspension according to one embodiment. [Figure 22] This figure shows the components of a kit for preparing a cell suspension according to one embodiment. [Figure 23] This figure shows the components of a kit for preparing a cell suspension according to one embodiment. [Figure 24]This figure shows the components of a kit for preparing a cell suspension according to one embodiment. [Figure 25] This is a perspective view of a preparation tray according to one embodiment. [Figure 26] This is a cross-sectional view of a preparation tray according to one embodiment. [Figure 27] This is a perspective view of a preparation tray according to one embodiment. [Figure 28] This is a cross-sectional view of a preparation tray according to one embodiment. [Figure 29] This is a perspective view of a preparation tray according to one embodiment. [Figure 30] This is a cross-sectional view of a preparation tray according to one embodiment. [Figure 31] This is a perspective view of a preparation tray according to one embodiment. [Figure 32] This is a cross-sectional view of a preparation tray according to one embodiment. [Figure 33] This is a perspective view of a preparation tray according to one embodiment. [Figure 34] This is a cross-sectional view of a preparation tray according to one embodiment. [Figure 35] This is a perspective view of a preparation tray according to one embodiment. [Figure 36] This is a perspective view of a preparation tray according to one embodiment. [Figure 37] This is a perspective view of a preparation tray according to one embodiment. [Figure 38] This is a perspective view of a preparation tray according to one embodiment. [Figure 39] This is a partial cross-sectional view of a preparation tray according to one embodiment. [Figure 40] This is a partial cross-sectional view of a preparation tray according to one embodiment. [Figure 41] This is a partial cross-sectional view of a preparation tray according to one embodiment. [Figure 42] This is a perspective view of a kit for preparing a cell suspension according to one embodiment. [Figure 43] This is a perspective view of a kit for preparing a cell suspension according to one embodiment. [Figure 44] This is a perspective view of a kit for preparing a cell suspension according to one embodiment. [Figure 45] This is an image of the step of packaging a transport kit according to one embodiment. [Figure 46] This is an image of the step of packaging a transport kit according to one embodiment. [Figure 47] This is an image of the step of packaging a transport kit according to one embodiment. [Figure 48] This is an image of the step of packaging a transport kit according to one embodiment. [Figure 49] This is an image of the step of packaging a transport kit according to one embodiment. [Figure 50] This is an image of the step of packaging a transport kit according to one embodiment. [Figure 51] This is an image of the step of packaging a transport kit according to one embodiment. [Figure 52] This is an image of the step of packaging a transport kit according to one embodiment. [Figure 53] This is an image of the step of packaging a transport kit according to one embodiment. [Figure 54] This is an image of the step of packaging a transport kit according to one embodiment. [Figure 55] This is an image of the step of packaging a transport kit according to one embodiment. [Figure 56] This is an image of the step of packaging a transport kit according to one embodiment. [Figure 57] This is an image of the step of packaging a transport kit according to one embodiment. [Figure 58] This is an image of the step of packaging a transport kit according to one embodiment. [Figure 59] This is a partial top view of an apparatus for preparing a cell suspension according to one embodiment. [Figure 60A]This is an exploded view (including the housing liner) of the housing of a kit for preparing a cell suspension according to one embodiment. [Figure 60B] This is a plan view (excluding the housing liner) of the housing of a kit for preparing a cell suspension according to one embodiment. [Figure 61A] This is a perspective view of the base of a kit for preparing and / or supplying a cell suspension according to one embodiment. [Figure 61B] This is a rear view of the base of a kit for preparing and / or supplying a cell suspension according to one embodiment. [Figure 61C] This is a plan view of the base of a kit for preparing and / or supplying a cell suspension according to one embodiment. [Figure 61D] This is a front view of the base of a kit for preparing and / or supplying a cell suspension according to one embodiment. [Figure 61E] This is a bottom view of the base of a kit for preparing and / or supplying a cell suspension according to one embodiment. [Figure 61F] This is a left side view of the base of a kit for preparing and / or supplying a cell suspension according to one embodiment. [Figure 61G] This is a right-side view of the base of a kit for preparing and / or supplying a cell suspension according to one embodiment. [Figure 62A] This is a perspective view of an integrated tray portion of a kit for preparing and / or supplying a cell suspension, according to one embodiment. [Figure 62B] This is a rear view of an integrated tray section of a kit for preparing and / or supplying a cell suspension, according to one embodiment. [Figure 62C] This is a plan view of an integrated tray section of a kit for preparing and / or supplying a cell suspension, according to one embodiment. [Figure 62D] This is a front view of an integrated tray section of a kit for preparing and / or supplying a cell suspension, according to one embodiment. [Figure 62E]This is a bottom view of an integrated tray section of a kit for preparing and / or supplying a cell suspension, according to one embodiment. [Figure 62F] This is a left side view of an integrated tray section of a kit for preparing and / or supplying a cell suspension, according to one embodiment. [Figure 62G] This is a right-side view of an integrated tray section of a kit for preparing and / or supplying a cell suspension, according to one embodiment. [Figure 63A] This is a perspective view of the housing portion of a kit for preparing and / or supplying a cell suspension according to one embodiment. [Figure 63B] This is a rear view of the housing portion of a kit for preparing and / or supplying a cell suspension, according to one embodiment. [Figure 63C] This is a plan view of the housing portion of a kit for preparing and / or supplying a cell suspension, according to one embodiment. [Figure 63D] This is a front view of the housing portion of a kit for preparing and / or supplying a cell suspension, according to one embodiment. [Figure 63E] This is a bottom view of the housing of a kit for preparing and / or supplying a cell suspension, according to one embodiment. [Figure 63F] This is a left side view of the housing portion of a kit for preparing and / or supplying a cell suspension, according to one embodiment. [Figure 63G] This is a right-side view of the housing portion of a kit for preparing and / or supplying a cell suspension, according to one embodiment. [Modes for carrying out the invention]

[0008] In some embodiments, the kit for preparing the cell suspension includes the apparatus and how It may be equipped with a jigging. The housing is for, for example, storing and / or transporting the device. The device may also function as a packaging device for tissue processing. The apparatus may specify one or more containers to be used. For example, the apparatus may specify a first container And a second container may be specified. The apparatus is used in the first part of the tissue processing method. A first label for identifying the first container, and a second container for use in the second part of the tissue processing method. A second label to identify the device may be included. The housing is designed to receive the device. It may be configured as follows: The housing is associated with the first part of the tissue processing method. Even if it includes a first housing section configured to house a set of components Good. The first housing part is a first visual indicator associated with the first label of the device. It may include a cater. The second housing part is associated with the second part of the tissue processing method. It may be configured to store a second set of components. The woving section includes a second visual indicator associated with a second label of the device. It may be. In some embodiments, the first housing portion is the device It is identified as containing a first set of components associated with a first container. It may be as follows, and the first container of the first set of components and apparatus is for tissue processing It may be used in the first part of the method. Similarly, in some embodiments In this configuration, the second housing portion comprises a second set of components associated with the second container of the device. It may be identified as containing a component, and a second set of components The second container of the net and apparatus is to be used in the second part of the tissue processing method. It may be. In a similar manner associated with other parts of the tissue processing method, any suitable number ( For example, three, four, or more housing parts and each container are identified. They may be labeled. Also, in some embodiments, As an addition or alternative, corresponding labels and visual indicators can be used to make one or more This relates to the outer surface (e.g., surface) of the non-container device in a similar manner to multiple housing parts. It is understood that this may be labeled.

[0009] In some embodiments, packaging for an apparatus for preparing cell suspensions The stem may comprise a first housing section and a second housing section. The device is Even if it includes a first container having a first label and a second container having a second label Good. The first housing part is associated with the first part of the tissue processing method, a first set A set of one or more recesses configured to receive one or more components The parts may be defined. The first housing part is associated with the first label of the device. It may include a first visual indicator (for example, this allows the first capacity of the device) To identify the first housing portion as including components associated with the vessel. However, the first container of such a first set of components and apparatus is the organization (May be used in the first part of the processing method). The second housing part is, A second set of one or more components associated with the second part of the tissue processing method A set of one or more recesses configured to receive a to may be defined. The housing portion includes a second visual indicator associated with a second label of the device. It may be (for example, this may result in the component associated with the second container of the device The second housing portion may be identified as including the second A set of components and a second container of the apparatus are used in the second part of the tissue processing method. (It may be possible to do so in a similar manner to other parts of the tissue processing method.) and any suitable number (for example, 3, 4, or more) housing parts and Each container may be identified and labeled. Also, some actual In terms of application methods, this may be added or replaced by corresponding labels and visual indicators. The outer surface of the non-container device by a similar method relating to one or more housing parts. It is understood that (for example, the surface) may be labeled.

[0010] In some embodiments, a device packaged in a housing is used for cell suspension. A method for preparing turbidity involves a first label and a first container of the apparatus, which are positioned close to the first container of the apparatus. First matching with the first visual indicator included in the first housing portion of the wug This includes separating. In response to the identification of the first matching, a first set of the first housing portion Remove the components and associate them with the first container using the first set of components. The first part of the tissue processing method may be performed. Also, the second part of the apparatus A second label positioned in close proximity to the container and a second housing portion included in the second housing of the housing The second match may be identified with the visual indicator. In response, remove the second set of components from the second housing section, and the second set Using the components, the second part of the tissue processing method associated with the second container is implemented. It may be done in some embodiments. In some embodiments, this method may be done in any number of suitable cases. For example, identifying the matching of three, four, or more housing sections with their respective containers. Similarly, components are removed from these housing sections and subjected to tissue processing. It may also include using it in other parts of the law. In some embodiments, Therefore, as an addition or alternative, corresponding labels and visual indicators are provided. In a similar manner relating to one or more housing parts, the outer surface of the non-container device (for example, It is understood that the surface may be labeled.

[0011] Kits and apparatus for cell suspensions Figure 1 is a schematic diagram of Kit 100 for preparing a cell suspension. Kit 100 is, The system comprises a device 110 and a housing 120. The device 110 may also be referred to as a processing unit. Generally, the apparatus 110 is used, as detailed below, (for example, to prepare cell suspensions) Features used in tissue processing methods (for example, containers, preparation trays for tissue manipulation, etc.) ) may include. On the other hand, the housing 120 is connected to the device 110 and / or device 11 The various components (e.g., tools) used in organizational processing methods that involve 0 It may also function as packaging. Additional housing and device Exemplary characteristics will be explained in more detail below.

[0012] For example, the apparatus 110 may include a first container 111 and a second container 113. i. The apparatus 110 identifies the first container 111 to be used in the first part of the tissue processing method. Label 112 identifies the second container 113 used in the second part of the tissue processing method. It may include a second label 114.

[0013] The housing 120 may be configured to receive the device 110. 120 may include a first housing portion 130 and a second housing portion 140. i. The first housing portion 130 is associated with the first part of the tissue processing method. It may be configured to store the set of components. Second housing section 140 This stores a second set of components associated with the second part of the tissue processing method. It may be configured as follows: The first housing portion 130 is the first label 112 of the device. It may include a first visual indicator 132 associated with the second housing. The part 140 is a second visual indicator 1 associated with the second label 114 of the device. 42 may be included. One or both of the first and second housing parts are tissue-treated. Each method accepts a specific type of component (e.g., a tool) to be used. This may include size specifications and molded recesses.

[0014] In some embodiments, the first housing portion 130 and the second housing portion 1 40 may be integrally formed as part of the integrated tray section 150. In this embodiment, the housing 120 comprises a first housing portion 130 and a second housing portion Includes an integrated tray section 150 configured to removably receive the sizing section 140. The first housing portion 130 and the second housing portion 140 may be integral to each other. The integrated tray section 150 may be formed as a single unit or as separate tray sections. to receive the first housing portion 130 and / or the second housing portion 140 One or more recesses may be defined. Also, in some embodiments The integrated tray section 150 defines a recess configured to receive the device 110. It's okay to be there.

[0015] In some embodiments, the first housing portion 130 and the second housing portion 1 40 may be detachably coupled to one another. For example, in some embodiments In this configuration, the housing portion 130 and the second housing 140 have multiple perforations (perfor The first housing portion 130 is separated (for example, by tearing) the ations A perforated connector can be separated from the housing portion 140. They may be joined to each other via the ing portion. Another example is several implement forms In this state, one or more fasteners (for example, adhesives, mechanical fasteners, etc.) to / or a mating or interlocking mechanism (for example, the first housing portion 130 is a first mating machine) The structure may include the second housing portion 140 which may include the second fitting mechanism, and the first and (The second mating mechanism is connected to each other in a snap-fit ​​or detachable manner.) The housing portion 130 and the second housing 140 may be connected to each other.

[0016] In some embodiments, the housing 120 includes a third housing portion 160. The apparatus 110 may also specify a third container 115, and also tissue processing. The method includes a third label 116 that identifies the third container 115 used in the third part of the method. The third housing portion 160 is associated with the third part of the tissue processing method. It is configured to store a set of 3 components. For example, several implementations In one embodiment, the third housing portion 160 has a recess in each of the third set of components. The parts may be defined. In some embodiments, the third housing part 160 is The recesses of various components of a third set of components may also be defined (even If, then, a single recess is configured to accept a subset of common or identical components. (This may be done.) The third housing portion 160 is associated with the third label 116 of the device. It may also include a third visual indicator 162. In some embodiments, The third housing portion 160 is (for example, above with respect to the first and second housing portions) As mentioned above, separating multiple perforations (for example, by tearing them) Therefore the third housing portion 160 is the first housing portion 130 and / or the second housing portion A perforated connecting portion (a perforated connecting portion) is provided so that it can be separated from the wedge portion 140. via (rtion), and / or one or more fasteners, mating, or interlocking devices. (Through the mechanism) the first housing portion 130 and / or the second housing portion 140 They may be detachably coupled. In some embodiments, housing 1 20 is any other suitable number (for example, 2, 3, 4, 5, or more). It may also be equipped with a housing section, and the outside of the device used in each part of the tissue processing method It is understood that each label on the surface corresponds to a corresponding visual indicator.

[0017] In some embodiments, the housing 120 is the first housing portion 1 shown in Figure 1. 30, a second housing section 140, and a third housing section 160, and various other housings It may include a base 170 configured to receive the zing portion. The base 170 is Housing section 130, housing section 240, and housing section 360 In addition, it may be configured to receive the device 110. In some embodiments The base portion 170 is an integrated tray portion 150 (for example, the first housing portion 130, the second The housing portion 140 and the integrated tray portion 150 including the device 110, and the third The housing portion 160 may be configured to receive the base portion 170. For example, the base portion 170 is A first recess configured to receive the integrated tray portion 150, and a third housing portion 1 A second recess configured to receive 60 may be defined. Therefore, In some embodiments, the integrated tray portion 150 (first housing portion 130, second The housing portion 140 and the device 110, and the third housing portion 160 Each is removed individually from the base 170, allowing the user to access the integrated tray section 15. 0 and the device 110 can be easily and efficiently removed from the base, and the third housing portion 160 It may be possible to store it in a separate area.

[0018] In some embodiments, the housing 120 is integrated with the base 170 (for example, A box on which a base portion 170 (including a ray portion 150 and a third housing portion 160) can be installed. Box 108 may comprise other suitable enclosures. Box 108 may comprise, for example, a housing A suitable enclosure for partially or entirely surrounding other parts of the 120 (e.g., trays, etc.) It may include cardboard that has been folded and / or otherwise secured to the body. In one embodiment, box 108 (or a box insert placed inside) (T) may specify the vial recess 180. The vial recess 180 is a pull-out type It may be placed in a vial, insert, or other structure, and may also be an enzyme vial. Even if it is configured to receive (for example, an enzyme vial placed in a sterile pouch) Good. Enzymes, as will be explained in more detail below, for example, to chemically break down tissues. Suitable. In some embodiments, the housing 120 is the first housing portion 13 0, second housing section 140, device 110, and optional third housing A partition may be provided to separate the vial recess 180 from the part 160. For example, The cut may separate the vial recess 180 from the base 170. For example, sterilization of other parts of the housing 120 (or at least the device 110 This allows for the sterilization of enzyme vials separately from the sterilization process. Therefore, the enzyme vials and Other parts of the housing 120 (for example, the device 110) undergo different sterilization processes. It may come into use. Such separate sterilization processes may include, for example, enzymes. This may help to avoid damage or other malfunctions to the device 110. For example, several In one embodiment, the enzyme is at risk of being damaged when subjected to ethylene oxide sterilization. On the other hand, device 110 (may include a processor and / or other electronic equipment) ) may be at risk of damage if subjected to gamma radiation sterilization. Therefore, enzyme Each vial and device 110 reduces the risk of damage to the sterile components. It may be subjected to a sterilization process. For example, the electronics (for example, of device 110) The portion of the housing 120 including the container is subjected to a first sterilization process (for example, by ethylene oxide). While sterilized by the enzyme vial, the vial recess 180 of the housing 120 Before insertion, it is separately sterilized by a second sterilization process (e.g., by gamma-ray radiation). They may be sterilized. In some embodiments, the sterile enzyme vial is It may be prepared to be placed in a sterile pouch before being placed in the vial recess 180. And, before the first part of the tissue processing method or in an initial step, yeast in the sterile pouch The raw vial is removed from the vial recess 180, and the enzyme vial is removed from the sterile pouch. The enzyme vial is then placed together with a first set of components within the first housing portion 130. So that it can be transferred (for example, to a workspace near or within the sterile field), The enzyme vial is disposed within the enzyme vial recess defined in the first housing portion 130. It's okay if it's made possible.

[0019] In some embodiments, the apparatus 110 can be used to perform a tissue processing method. The apparatus 110 may include any preferred features or components. For example, The structure 110 may include a skin tissue manipulation area enclosed by side walls. A may optionally include a textured surface area. Several implementations In one embodiment, the apparatus 110 is used to perform at least a part of a tissue processing method (for example For example, preparations used in the second and / or third parts of the tissue processing method. It may be equipped with a tray. The preparation tray is removed from the complementary concave area of ​​the apparatus 110. An insert tray that can be lifted out of the slot (for example, by lifting it out, removing it, etc.) Alternatively, in some embodiments, the preparation tray may be integrated with the apparatus 110. They may be formed, for example, integrally formed as a region within the base structure of the device 110. They may be included. Furthermore, in some embodiments, the preparation tray may be one or more. Snap-off connector piece, perforated (perforated), release-type mating mechanism, removable. It is removably attached to the device 110 using an adhesive (for example, tape). It may also be the case that the preparation tray has a skin tissue manipulation area and It may include surrounding side walls. In some embodiments, the preparation tray is used for skin tissue One or more are provided on the other part of the bottom surface of the preparation tray, including the operating area. By including the collection area, the skin tissue handling area of ​​the preparation tray is accessible from the collection area side. The fluid may be allowed to flow into the call / collection area.

[0020] In some embodiments, the skin tissue manipulation area may be a smooth plane. In some embodiments, the skin tissue manipulation area is a collection area (for example, a filter (including the collection area) or toward the third container 115, one or more depressions, bulges, It may include, or may specify, slopes, inclines, or paths (e.g., outlets). i. In some embodiments, at least a portion of the preparation tray (for example, skin tissue) The working area (for example, by impregnation of the skin tissue working area by plastic injection molding) (It may be formed at least partially of an antimicrobial material.) In addition or as an alternative, At least a portion of the preparation tray (for example, the skin tissue manipulation area) is coated on the surface of the preparation tray. It may include a fabricated antimicrobial coating. For example, the preparation tray may contain sulfonamide drugs. One or more of the following: drugs, trimethoprim derivatives, quinolones, and / or nitrofuran derivatives. It may also contain antimicrobial agents. As another example, the preparation tray may contain, as an addition or alternative, antimicrobial agents. Bacterial nanotechnology or other antimicrobial technologies (e.g., silver nanoparticles, phenol, polybiguanide) For example, polyhexamethylene biguanide (PHMB), chitosan, and / or It may contain lamin (for example, N-haramine). As another example, the preparation tray is , adhesion through bonding (e.g., covalent bonds, ionic bonds) within the coating, and / or matrix One or more antibiotics (e.g., minocycline, rifampin, bacillus) are used in a steroid medication. (e.g., cincomycin, silver sulfadiazine, ceftazidime grafts, gentamicin grafts) It may include. As another example, preparation trays may be one or more as additions or replacements. A number of other suitable bacteriostatic or other antimicrobial coatings or substances (e.g., organosiloxanes) ) may include. As another example, the preparation tray may include impregnation and as an addition or alternative. A solution comprising one or more disinfectants (e.g., chlorhexidine) by coating / or application It may be. Furthermore, the preparation tray may, as an addition or replacement, be used for the materials in the preparation tray. One or more precious metals (e.g., silver salts, ions) incorporated and / or coated. It may also contain complexes and / or nanostructures of silver, gold, copper, or palladium, etc. i. As an addition or alternative, the preparation tray may contain one or more antibiotics or other antimicrobial substances. Methylene blue and / or phenothiazines, etc., which function to act synergistically with the quality. It may contain one or more non-antibiotics (by impregnation and / or coating).

[0021] In some embodiments, the apparatus 110 heats the contents of the first container 111. It may include a heating assembly configured as follows. The heating assembly may be any suitable Heating mechanism, energy storage device, indicator light (e.g., LED), one or more It may be equipped with multiple activation buttons and / or associated electronic devices. The heating assembly is configured to be activated by pressing a button on the heating assembly. It may be done. The heating assembly is a heating mechanism that heats up to the target temperature or target temperature range. It is configured to illuminate a first indicator light during a first period of heating. It is also possible. In some embodiments, the heating assembly has a heating mechanism that reaches a target temperature or target temperature. The second indicator light is to illuminate during the second period when the device is operating within the standard temperature range. It may be. In some embodiments, the heating assembly is the first container 111 and / Or, during the period when the contents of the first container 111 are at or within the target temperature range, the second container The indicator light may be turned on. In some embodiments, heated acetate The heating mechanism maintains the contents of the first container 111 at a target temperature or within a target range. To indicate that it is actively operating, the first indicator light is illuminated. The first indicator light may be configured to turn on and off periodically or intermittently. The heating assembly raises the temperature of the first vessel 111 for a first period (for example, 15 minutes, 20 minutes). It may be configured to be maintained for a period of minutes or 60 minutes. Subsequently, the heating assembly is heated to the target temperature over a second period (for example, 15 minutes). Alternatively, it may be configured to maintain within the target range. In some embodiments, The heating assembly then emits an audible signal at periodic intervals during the second period (for example, once every minute). It may also include an alarm (for example, an audible alarm) configured to give a certain signal. In some embodiments, after the first or second period, the heating assembly is The operation of supplying heat to container 111 may be automatically stopped.

[0022] In some embodiments, the first set of components is a certain volume of water (even It may also include vials containing sterile water, needles, and / or enzyme syringes. For example, the syringe may be a 10 ml syringe or any suitable syringe. The needle is ( For example, it can be attached to a syringe by a snap-fit, screw-in, or other suitable fastener. It may be configured as follows. In some embodiments, a first set of components This may include a cell filter. In some embodiments, a first set of components The ingredient may contain an enzyme vial. The first housing portion 130 is the first Each component of the set is associated with the shape of each of the first set of components. Recesses may be defined that are molded to complement the shape.

[0023] In some embodiments, a second set of components is a buffer vial (bu May include a ffer vial, needle, and / or buffer syringe. Several implementations In this embodiment, the second set of components is the first buffer vial, the second buffer vial, needle, first syringe (for example, a syringe intended for transferring buffer), second Syringes (for example, syringes intended for transferring unfiltered suspensions), and two surgical syringes It may contain s. The first syringe and the second syringe each hold, for example, 10 ml. Alternatively, a syringe of any suitable capacity may be used. The filling needle may be, for example, a blunt filling needle. A needle may be used. A surgical scalpel may be disposable. The first buffer vial is The second buffer may include, for example, 10 ml or any suitable volume. The favial may contain, for example, 30 ml or any suitable volume of buffer. i. The second housing section 140 is a second set of components, each or the same component Associated with a subset of components, each or identical of the second set of components. Recesses may be defined that are shaped to complement the shape of a subset of components. .

[0024] In some embodiments, a third set of components includes a buffer vial, fine Bone filter, one set of skin cell syringes, one set of needles, unfiltered suspension syringe, one set of spray nozzles, and may include a pair of scalpels. For example, a pair of skin cell syringes may contain four skin cells. It may include a cell syringe (for example, a 10 ml syringe). A set of needles has four prongs. It may include any suitable number of needles (for example, blunt filling needles). A set of sprays The mist nozzle may include four spray nozzles. Each skin cell injector may have (for example, (One-handed operation) One of a set of needles, or alternatively, one of a set of spray nozzles They may be bound together. The buffer vial may contain, for example, 30 ml or other suitable bodies. It may include a product buffer. In some embodiments, a third set of components The kit may include a set of skin cell syringes, a set of needles, and a set of spray nozzles. i. The third housing portion 160 is each of the second set of components or the same component Associated with a subset of components, each or identical of the second set of components. Recesses may be defined that are shaped to complement the shape of a subset of components. .

[0025] The visual indicators on the housing 120 and the labels on the outer surface of the device 110 are of any preference. It may be handled in an appropriate manner. In some embodiments, the first label 112 and Both the first visual indicator 132 and the first visual indicator 132 are the same alphanumeric character such as "A" (for example, A It may include B, C, 1, 2, 3, etc. In some embodiments, the first Both the bell 112 and the first visual indicator 132 are additions or alternatives to the above. and include the same color (for example, red, orange, yellow, green, blue, purple, etc.) This may also be the case. In some embodiments, the first label 112 and the first visual indicator Cata 132 can also include geometric symbols (e.g., circles, ellipses) as additions or alternatives. Shapes (triangles, squares, rectangles, etc.), punctuation marks, or other unique symbols (e.g., tildes, It may also include the same symbol as a zigzag line, etc. In some embodiments, the first Both the bell 112 and the first visual indicator 132 are letters, colors, numbers, and / may also contain the same combination of symbols.

[0026] Similarly, in some embodiments, the second label 114 and the second visual indicator Catalog 142 is above the first label 112 and the first visual indicator 132 As described above, it may also contain the same alphanumeric characters, colors, and / or symbols. For example, the second label 114 and the second visual indicator 142 both use the same alphanumeric characters. It may include a letter (for example, B). In some embodiments, the second label 1 Both 14 and the second visual indicator 142 contain the same color (for example, gray). It may be so. In some embodiments, the second label 114 and the second visual Indicator 142 all contain the same symbol. In some embodiments, the second Both label 114 and the second visual indicator 142 are (for example, the first ) Common letters, colors, numbers, or symbols of the bell 112 and the first visual indicator 132 Includes the same combination of letters, colors, numbers, and / or symbols (different from the above).

[0027] Similarly, in some embodiments, the third label 116 and the third visual indicator Catalog 162 is described above with respect to the first label 112 and the first indicator 132. Similarly, it may contain the same alphanumeric characters, colors, and / or symbols. For example, the Both label 116 and the third visual indicator 162 use the same alphanumeric characters ( For example, C) may be included. In some embodiments, the third label 116 Both the third visual indicator 162 and the third visual indicator 162 contain the same color (for example, green). This may also be the case. In some embodiments, the third label 116 and the third visual indicator Each of the caterers 162 may contain the same number (for example, 3). In this embodiment, both the third label 116 and the third visual indicator 162 are , may include the same symbol. In some embodiments, the third label 116 and The third visual indicator 162 is (for example, the first label 112 and Unlike the common letters, colors, numbers, or symbols of the first visual indicator 132, the second Common letters, colors, numbers, or symbols of label 114 and the second visual indicator 142 It may also include the same combination of letters, colors, numbers, and / or symbols (different from the number). stomach.

[0028] Any of the visual indicators described herein may be present on each outer surface of the device 110 (for example, Each outer surface (labeled in the same way as the corresponding housing) and / or tissue is treated. The housing part (and the components contained inside) corresponds to each part of the method. A texture shape or patterning (e.g., bump, well) that uniquely identifies the object. It is understood that other suitable forms may exist, such as ribs or recesses. As a replacement, some or all of the components included in the housing are each of the devices 110 Each part of the method for processing the outer surface and / or tissue is individually identified. It may be designed to do so. For example, some or all of the components may be housing Regarding the part, as mentioned above (for example, visual elements such as alphanumeric characters, colors, symbols, and textures) It may include a handle labeled with an indicator.

[0029] In some embodiments, at least a portion of the housing 120 (for example, a recess) The housing portion (including the housing) is at least partially injection molded, milled, or 3D printed. , or it may be formed through other suitable processes such as folding. Also, housing 1 At least a portion of the 20 is a suitable rigid or semi-rigid material (e.g., plastic, gold) It may include materials such as cardboard. For example, the first housing part 130, the second housing A housing section 140, an optional third housing section 160, and an optional integrated section The ray section 150, the optional base section 170, and the optional box section 108 are Each may be formed from any suitable material. For example, the first housing portion 1 30, the second housing section 140, and the third housing section 160 are each plus It may be formed from plastic (for example, translucent or transparent plastic). The tray portion 150 and the base portion 170 are each made of plastic (for example, translucent plastic). Box 108 may be made of cardboard, It may be formed of other suitable rigid or semi-rigid materials. In some embodiments, Therefore, by a single sterilization method (for example, ethylene oxide sterilization), Kit 100 One or more components may be sterilized.

[0030] Figures 60A and 60B show the kit for the preparation and / or application of cell suspensions. This document details one exemplary embodiment of a packaging system. Specifically, Figure Figures 60A and 60B show the base, integrated tray section (visual indicators "A" and "B"). In addition to the first and second housing sections accompanied by the ", including the device recess, as well as the visual input This shows an exemplary housing assembly including a third housing section with a dicator "C". The first, second, and third housing sections are each used for the preparation of cell suspensions and / or The first, second, and third parts of the method for application It includes recesses that receive various components in a snap-fit ​​manner. Figure 60A shows the first and a housing liner configured to be positioned above the second housing section. Figure 60B is an exploded view of the assembly shown above, while Figure 60B is an exploded view of the assembly excluding the housing liner. This indicates that

[0031] Figures 61A to 61G show exemplary housing assemblies as shown in Figures 60A and 60B. This shows the base in detail. Specifically, Figures 61A to 61G show the cell suspension Perspective view, rear view, top view, front view of the base of the kit for liquid preparation and / or supply. These are the bottom view, left view, and right view.

[0032] Figures 62A to 62G show exemplary housing assemblies as shown in Figures 60A and 60B. This shows the integrated tray section in detail. Specifically, Figures 62A to 62G are shown in the following order: Perspective view of the integrated tray section of the kit for preparing and / or supplying cell suspensions, rear view. The diagrams include a top view, front view, bottom view, left view, and right view.

[0033] Figures 63A to 63G show exemplary housing assemblies as shown in Figures 60A and 60B. This shows the third housing section in detail. Specifically, Figures 63A and 63B show it. A perspective view of the third housing section of the kit for preparing and / or supplying cell suspensions. The images are a front view, rear view, top view, front view, bottom view, left view, and right view.

[0034] Methods for preparing and applying cell suspensions Using the kits and devices described above, for the preparation and application of cell suspensions The organization may also implement methods such as organizational processing methods. Below are some examples of such methods. Let's explain the examples.

[0035] As described above, the organizational processing method consists of a first part, a second part, and an optional third part. The following parts may be included: the first part, the second part, and the third part of the tissue processing method. Before performing one of the following, the user may perform the first housing part 130, the second housing Either part 140, or the optional third housing part 160, is used in the tissue processing method. It may be possible to identify whether it is associated with a specific part. For example, the user, To do this, the first label 112, the second label 114, and the third label 116 One of them and the first visual indicator 132, the second visual indicator 142, and a match with one of the third visual indicators 162 is identified at least partially. It may be done in this way. For example, the user looks at the device 110 and the first container 111 and related After identifying the first label 112 attached, the first housing part 130, the second housing The corresponding first visual indicator in the 140 and third housing portion 160 An attempt may be made to identify the first visual indicator 132. By identifying that it is associated with the housing part 130, the user can identify the first housing part 130 Using the first set of components stored in the recess, the first part of the tissue processing method You may proceed to start. Follow the same procedure with respect to the second container 113, then the second container 1 A second visual indicator 142 corresponding to a second label 114 associated with 13 By identifying that the second housing portion 140 includes the second housing portion 140, the user can identify the second housing portion 140 Using a second set of components stored in the recess, the second part of the tissue processing method You may proceed to start. Following a similar procedure with respect to the third container 115, the third container 1 A third visual indicator 162 corresponding to a third label 116 associated with 15 By identifying that the third housing portion 160 is included, the user can identify the third housing portion 1 Using a third set of components stored in 60 recesses, the third part of the tissue processing method You may also set it to start in minutes.

[0036] In some embodiments, the first part of the tissue processing method is a yeast for the tissue processing method. The process includes preparing an element mixture and delivering the enzyme mixture to a first container 111. It may be. For example, the first housing part 130 is directed towards the target workspace (for example For example, from box 108 and / or integrated tray section 150 to the sterile field or its vicinity ) It may be designed to move. The cover of the enzyme vial is the enzyme in the first housing part. Remove from the vial recess, and optionally, remove the diaphragm from the vial with sterile alcohol. It may be possible to wipe it with a wipe and let it dry. (For example, the first set of components The needle may be fluidly coupled to the syringe of the first set of components. The needle of the component (for example, of the first set of components) inside the water vial Insert into the syringe and draw a certain volume of water (for example, the entire volume of the water vial) from the water vial into the syringe. You can also pour it in. A certain volume of water (for example, the total volume of water in the syringe) into the syringe or After injecting the enzyme into the vial, let it stand until the enzyme dissolves in water and forms an enzyme mixture. You can also mix it without shaking (for example, without shaking to avoid foaming). The enzyme mixture may be drawn back into the syringe. Then the enzyme mixture is put into the first apparatus 110 The contents may be dispensed into container 111. The syringe and needle may be discarded.

[0037] In some embodiments, the second part of the tissue processing method is a B Preparing a buffer (for example, drawing a certain volume of buffer from a buffer vial) This may include delivering to the second container 113. Even if a subset of the workspace moves from the non-sterile preparation area to the sterile field Good. In the sterile field, empty buffer injection (for example, of the second set of components) The injector may be optionally marked as "buffer," and an empty unfiltered suspension injection... The launcher (for example, if it is included in the second set of components) is optional as "unopened". It may be marked as "super-filtered suspension". An unfiltered suspension syringe is a third set of components. In this embodiment, the unfiltered suspension is used in the second part of the tissue processing method. They are not handled in this manner. In non-sterile preparation areas, covers (for example, a second set of) Remove the component from the buffer vial, and remove the diaphragm from the buffer vial. Optionally, the area may be wiped with a sterile alcohol wipe and dried. Sterile field In this case, the needle (of a second set of components) is connected to the buffer syringe. It is also acceptable. In a non-sterile preparation area, users in the sterile field may take the buffer vial. Buffer vials may be placed to allow for the extraction of a certain volume of buffer. Sterilization field The user may position the free end of the needle inside the buffer vial, and in some cases You could also draw a buffer for the product (for example, a buffer for the total product) into the syringe. In the bacterial field, a certain volume of buffer is dispensed into the second container 113 of the apparatus 110. You may do so. Alternatively, you may keep the syringe with the needle attached within the sterile field.

[0038] In some embodiments, a third part of the tissue processing method involves delivering the cell suspension to the patient. Preparing the cells (for example, by dissecting a skin sample and then extracting the cells from the dissected skin sample) This involves generating a cell suspension containing [the specified substance] and drawing the cell suspension into a skin cell syringe. This is also fine. A set of skin cell syringes (for example, from a third set of components) For example, four skin cell injectors), a set of needles, and a set of spray nozzles (for example, third (After being removed from the housing portion 160 or from the third housing portion 160) Sterilized field It may be arranged to be placed there. A wound bed for the patient may be prepared. For example, wound Clean the wound bed (e.g., rotary diamond head deburring, laser ablation) Angiogenesis may be performed (by sharp dissection or other alternative techniques). Also, prophylactic Approximately 48 optional administrations of antibiotics and / or tissue processing methods or procedures. You may want to disinfect the wound beforehand. Remove all necrotic tissue and treat petechiae. It may be observed. Punctate bleeding may occur at the donor site of the patient, and a skin sample may be extracted from the donor site. The skin sample may be approximately 0.15 - 0.20 mm thick. The skin sample may be a thin split - thickness skin sample. In some embodiments, a dermatome or a similar device may be used to obtain the skin sample.

[0039] In some embodiments, the area of the skin sample may be correlated with the estimated cell suspension volume and / or the skin treatment area. For example, in some embodiments, using a kit 100 for preparing a cell suspension, 1 ml of cell suspension may be generated from a 1 - square - centimeter skin sample. 1 milliliter of cell suspension may be disposed on an area of approximately 10 cm to approximately 100 cm to approximately 100 cm 2 2 to approximately 100 cm 2 2 to approximately 100 cm 2 2 or up to approximately 25 cm 2 2 or up to approximately 75 cm 2 or up to approximately 100 cm of the treatment area. For example, in some embodiments, 1 milliliter of cell suspension may be disposed on a treatment area of up to approximately 80 cm 2 (for example, enabling an expansion ratio of up to approximately 1:80). Thus, approximately 6 ml of cell suspension may be obtained from a 6 - cm [[ID=​​​​​​​​​​​​​​​​​​skin The sample may be processed to produce up to 24 ml of cell suspension. A 24 ml cell suspension can reach a maximum of approximately 1,920 cm³. 2 It can be used for treating the treatment area. For example, Table 1 shows the results obtained for treating various treatment area sizes using Kit 100. These are just a few examples, including the size of skin samples.

[0040] [Table 1]

[0041] Using the apparatus 110, the enzyme mixture in the first container 111 (for example, the apparatus 110 described above) Heating may be performed by the heating assembly. For example, the starting box on the device 110 The heating of the enzyme mixture in the first container 111 may be initiated by pressing the tongue. When the enzyme mixture has been heated to the target temperature, the first light indicator (for example, orange) will light up. The warm-colored lights may be turned on. The enzyme mixture reaches the target temperature. Then, the second light indicator (for example, a green confirmation light) will light up. It may be. In some embodiments, heating the enzyme mixture to the target temperature is It may be implemented in approximately 3 minutes or other suitable periods. In this embodiment, the first light indicator is a heating element to maintain the temperature of the enzyme mixture. It blinks briefly to indicate that the element has been activated intermittently or periodically. That's good too.

[0042] One or more skin samples (for example, each 6 cm) 2 The following sizes are available Two skin samples are placed in the first container 111, and the enzyme mixture is used to facilitate protein-protein interactions. To allow for decomposition, the enzyme mixture may be immersed in a heated enzyme mixture over a first period of time. The first period may be, for example, about 15 minutes to about 20 minutes. Simple samples may be cultured for longer periods (for example, up to 60 minutes). In some embodiments, this could be every 5 minutes, 10 minutes, 15 minutes, 20 minutes, 3 At intervals of 0 minutes, etc., the device 110 will provide an audible and / or visual alarm notification. It may be so. As an addition or alternative, after the completion of the first period (for example, approximately 1 Auditory and / or visual alarms are notified at different frequencies (e.g., 5 minutes to approximately 20 minutes). After providing the signal, the following period of time (e.g., every minute, every 5 minutes) is shown over a second period. You may also do this after a certain amount of time (for example, after 15 minutes, 30 minutes, 60 minutes, etc.) When device 110 stops, the heating assembly stops heating the enzyme mixture. It is also acceptable to use a voice. Examples of auditory alarms include sound effects (beeps, tones, etc.). Voice time display (e.g., "15 minutes") and voice instructions (e.g., "S"). Examples of visual alarms include "remove sample" or "check sample". This includes the activation of lights (e.g., LEDs) and text messages (e.g., on device 110). Knowledge is one example. Alarms that convey these notifications can be in any suitable form (e.g., auditory). (This may include visual, tactile, etc.) and / or third-party devices that provide notification. It is transmitted to a device (for example, a monitor screen, a mobile computer device). It is understood that this may be permitted.

[0043] In some embodiments, at least a portion of the second part of the tissue processing method is used for tissue processing. The third part of the method may be performed simultaneously. For example, the first container 111 While culturing the skin sample in the enzyme mixture, the buffer is delivered to the second container 113. You may do so.

[0044] Remove the skin sample from the heated enzyme mixture and adjust the device 110 with the dermis side down. They may be placed on a tray. For example, a third set of sterile forceps components. Using a knife or scalpel, remove the skin sample from the first container 111 and prepare it. It may also be placed on (I). The epidermal edge of each skin sample is lightly scraped with a scalpel, and the cells are found to be You may also test whether it degrades (for example, whether the epidermal cells separate easily). If the cells are breaking down, you can stop rubbing. If the cells are not breaking down, the skin The sample is returned to the heated enzyme mixture for a period of time (for example, about 5-10 minutes). Afterward, the sample is removed and an additional scraping test is performed to determine if the cells are broken down. This is also acceptable. If cell scraping is possible, place the skin sample in the second container 113. You may also immerse the skin sample in buffer to wash away any remaining enzyme mixture. Furthermore, after passing the degradation test, if necessary, a second cultured skin sample is placed in a second container. It may be placed in container 113. Third skin sample and / or fourth skin sample If pull processing is desired, the first and second skin samples in the second container 113 During the washing process, the third and fourth skin samples are placed in the first container 111. Alternatively, you can place it in a container and immerse it in the enzyme mixture.

[0045] (For example, the second set of components) from the second buffer vial The component's buffer syringe may be configured to draw in a volume of buffer. A certain volume of buffer contains, for example, 1 square centimeter of the first skin sample. Approximately 1 ml of buffer plus an additional amount to account for losses during processing (for example, about 0.5 ml). ) may include buffer and . Table 2 shows syringe volume and skin sample size. The starting buffer volume and resulting suspension volume for various treatment surface area sizes This shows an example of the total volume. As mentioned above, in some embodiments, 1 milliliter of cells The suspension is approximately 10 cm 2 ~approximately 100cm 2 Approximately 25cm 2 ~approximately 100c m 2 Approximately 50cm 2 ~approximately 100cm 2 , or up to approximately 25 cm 2 , maximum 50cm 2 up to approximately 75cm 2 , or up to approximately 100cm 2 Distributed to the treatment area It may be configured as follows. In some embodiments, 1 ml of suspension is heated to a maximum of 80°C. m 2 Used for treating treatment areas (for example, allowing for an expansion ratio of up to approximately 1:80) This may be done. In some embodiments, the volume of the cell suspension to be applied is applied It can vary depending on the method. For example, in some embodiments, in the case of spraying, 2m The suspension volume of l or more may be applied to the treatment area. In this embodiment, when using the dropper method, a suspension volume of less than 1 ml is applied to the treatment area. You may do so.

[0046] [Table 2]

[0047] Remove the skin sample from the second container 113 and prepare it (for example, with the dermis side down). It may be placed on a tray. A few drops of buffer from the buffer syringe onto the skin sample. It may be applied to fix the skin sample using forceps or other suitable instruments. By doing so, the surface of the epidermis may be lightly scraped with the scalpel blade. Once the cell suspension is in place, rub even harder until the remaining dermis is almost completely broken down. Alternatively, the first syringe can be used to collect the cell suspension into the collection area of ​​the preparation tray. The remaining buffer may be used to clean the scalpel and tray. For example, When the device 110, including the tray, is placed on a horizontal surface, it may be the area below other parts of the tray. You may collect the cell suspension in the corner of the tray. If necessary, tilt the tray. This may be done to allow the cell suspension to accumulate in the corners. Various preparation trays Exemplary features of the embodiment (for example, texture features, height features, etc.) are described below. This will be explained in more detail. You may keep the buffer syringe for later use.

[0048] Draw the cell suspension into an unfiltered suspension syringe (for example, a third set of components). After mixing, the cells may be delivered to a preparation tray and the preparation tray may be washed. By repeatedly lifting, delivering, and washing the tray, the maximum amount of cells can be collected. Both are then drawn into the unfiltered suspension syringe, at least a portion (e.g., substantially all) of the cell suspension. If not provided in the third container 115, a cell filter (e.g., of the third set of components) may be placed in the third container 115. It may be placed in the third container 115. The cell suspension may be dispensed from the unfiltered suspension syringe through the cell filter into the third container 115. The cell filter may be set to a filter size suitable for removing large microparticles to prevent nozzle blockage when spraying the cell suspension onto the treatment surface. For example, in some embodiments, the cell filter may have a filter size of less than approximately 200 μm, less than approximately 150 μm, less than approximately 100 μm, less than approximately 50 μm, or other suitable filter sizes. The unfiltered suspension syringe may be placed in a sterile field for subsequent use associated with any remaining skin sample. After passing the cell suspension through the cell filter, the cell filter may be removed from the third container 115. Optionally, any droplets of the cell suspension may be released into the third container 115 by tapping the cell filter above the third container 115. [[ID=E18]]If the cell suspension is clogged (e.g., when processing multiple skin samples), the cell suspension may be drawn back from the cell filter into the unfiltered suspension syringe, and a new cell filter (e.g., from another kit 100) may be installed in the third container 115 to perform additional filtering, If the cell suspension is clogged (e.g., when processing multiple skin samples), the cell suspension may be drawn back from the cell filter into the unfiltered suspension syringe, and a new cell filter (e.g., from another kit 100) may be installed in the third container 115 to perform additional filtering. If the cell suspension is clogged (e.g., when processing multiple skin samples), the cell suspension may be drawn back from the cell filter into the unfiltered suspension syringe, and a new cell filter (e.g., from another kit 100) may be installed in the third container 115 to perform additional filtering. If the cell suspension is clogged (e.g., when processing multiple skin samples), the cell suspension may be drawn back from the cell filter into the unfiltered suspension syringe, and a new cell filter (e.g., from another kit 100) may be installed in the third container 115 to perform additional filtering. If the cell suspension is clogged (e.g., when processing multiple skin samples), the cell suspension may be drawn back from the cell filter into the unfiltered suspension syringe, and a new cell filter (e.g., from another kit 100) may be installed in the third container 115 to perform additional filtering. <000The needle (e.g., of the third set of components) may be coupled to a skin cell syringe (e.g., of the third set of components). The filtered cell suspension in the third container 115

[0049] (e.g., of the third set of components) may be coupled to a skin cell syringe (e.g., of the third set of components). The filtered cell suspension in the third container 115 (e.g., of the third set of components) may be coupled to a skin cell syringe (e.g., of the third set of components). The filtered cell suspension in the third container 115 The turbidity may be drawn into the skin cell syringe. The third container 115 contains filtered cells. For collecting the suspension and drawing the filtered cell suspension into a needle attached to a skin cell syringe. It may be molded to include a useful conical base. The filtered cell suspension can then be applied to the therapeutic surface. You may keep the skin cell syringe aside for future use. Scrap the skin sample to extract skin cells. The steps involve generating an unfiltered suspension and washing the tray to maximize cell collection. The steps include passing the cell suspension through a filter and then using the filtered cell suspension (for example, The third step involves drawing the skin cells into the dermal cell syringe and the skin sample. Each step may be repeated to produce a skin cell syringe of filtered cell suspension.

[0050] In some embodiments, the treatment surface of the filtered cell suspension (e.g., wound bed, color) Prior to delivery to areas with untreated deposits, etc., prepare one or more dressings. You may do so. Cut each bandage to the appropriate shape and / or size, Prior to applying the cell suspension, apply (for example, surgical adhesive) to the underside or near the treatment surface. (By fixation or retention using sutures or staples) and from the treatment side You may want to reduce the outflow. After that, you may apply the cell suspension to the treatment surface. In some embodiments, the cell suspension is applied directly to a localized wound. Alternatively, it may be applied to full-thickness wounds in combination with a mesh-like autograft. That's good too.

[0051] Applying the prepared cell suspension to the patient's treatment area can be carried out in various ways. For example, depending on the volume of the cell suspension to be applied and the size of the treatment surface, the cell suspension may be adjusted. It may be sprayed onto the treatment surface or dropped. Prior to application, The skin cell syringe may be inverted multiple times to ensure that the turbidity is applied uniformly.

[0052] When applying a cell suspension by spray, remove the needle from the skin cell syringe containing the cell suspension. You may do this. Instead of a needle, a set of sprays (for example, a third set of components) One of the mist nozzles may be attached to a skin cell syringe. The mist nozzle is for the suspension Oriented toward the treatment surface, in a position where the first drop can be dropped onto the treatment surface, It may be positioned above the treatment surface (for example, approximately 10 cm from the highest point of the treatment surface). (May be installed). Apply pressure to the plunger of the skin cell syringe and inject the cell suspension into the treatment surface. By spraying onto the highest part, any spillage will cover the lower areas of the treatment surface. Alternatively, the cell suspension may be sprayed onto the treatment surface as a mist. For large treatment areas, when spraying, the sprayer should be operated in a continuous motion from one side to the other (for example, The combination of the spray nozzle and the skin cell syringe may be moved.

[0053] When applied by drop, the needle may be left in the skin cell syringe containing the cell suspension. In one embodiment, the cell suspension is poured onto the treatment surface, starting from the highest part of the treatment surface. By deliberately dripping the material, any spillage may be allowed to cover lower areas of the treatment surface. .

[0054] After applying the cell suspension, cover the treatment area with one or more dressings. This may be done. The dressing may be a non-adherent, non-absorbent, and / or small-pored dressing. In some embodiments, before covering the treatment surface, the dressing is lightly soaked in sterile saline This may be done. The dressing may be fixed to the treatment surface by a surgical adhesive, suture, or staple, or by other suitable fasteners. A secondary dressing may be placed on top of the primary dressing. The secondary dressing may have moderate absorbency, minimal adhesiveness, low shear, and be easily removable. Absorbent gauze may be placed on top of the secondary dressing.

[0055] In some embodiments, kit 100 may include a mechanism configured to allow removal of the vial cap, needle sheath, and / or syringe cap. For example, kit 100 may include a stopper removal feature. In some embodiments when the pull tab on housing 120 is pulled, the seal breaks and access to the interior of the packaging layer is enabled. Kit 100 may include an integral protective packaging layer coupled to a removable seal of the packaging layer that includes a pull tab. The packaging layer may include an observation window (e.g., a transparent area through which a user can view the interior of the packaging). The packaging layer may include instructions for use (e.g., may be molded into the packaging layer or printed on the packaging layer). In some embodiments, base 170, integrated tray portion 150, first housing portion 130, second housing portion 140, and / or third housing portion 160 may be included in an integral protective packaging layer and / or may be collectively enclosed by an integral protective packaging layer.

[0056] Figure 2 is a schematic diagram of an exemplary kit 200 for preparing a cell suspension. Kit 2 00 may be identical or similar in structure and / or function to the kit 100 described above. For example, kit 200 comprises a housing 220 and a device 210, but these The housing 120 and the device 110 are identical in structure and / or function to each other. The same may apply. Housing 220 is a box 208, first housing portion 230 , second housing section 240, third housing section 260, and integrated tray section 250 These include, however, box 108 and first housing portion 130 as described above in Figure 1. , second housing section 140, third housing section 160, and integrated tray section 150 The structure and / or function may be identical or similar. For example, housing 22 0 is optionally a vial recess 280 configured to receive vial 282. It may also be specified. Vial 282 contains enzymes (e.g., trypsin, trypsin- EDTA, dispase, collagenase, thermolysin, pronase, hyaluronidase pancreatin, elastase, papain, etc., dissociate the cell layers in skin tissue samples. It may also contain enzymes suitable for [the purpose of] [the function The second housing portion 240 includes a target indicator 232, and the second visual indicator The third housing portion 260 includes a third visual indicator 262, which includes a third visual indicator 262. First visual indicator 232, second visual indicator 242, and third visual Each of the target indicators 262 is installed in the same manner as described above with respect to kit 100 in Figure 1. This corresponds to the first label, second label, and third label (not shown) of the 210. It is composed of sea urchin.

[0057] As shown in Figure 2, the first set of components associated with the first part of the tissue processing method The net 231 may be disposed in the first housing portion 230, and the second tissue processing method A second set of components 241 associated with the second housing portion 240 It may be arranged in a third set of components associated with the third part of the tissue processing method. The component 261 may be located in the third housing section 260.

[0058] The first set of components 231 is a water bath containing a certain volume of water (for example, sterile water). The device includes an ial 233, a needle 234, and an enzyme syringe 235. For example, enzyme syringe 235 This may be a 10 ml syringe. The needle 234 is to be attached to the enzyme syringe 235. It may be configured as follows. As shown in Figure 2, the first housing portion 230 is (for example (To transfer enzyme vial 282 from box 208 to the workspace) A recess 237 configured to receive the 282 may be defined. Package 241 includes vial 243 containing buffer, needle 244, and buffer injection. The device 245 may be included. The needle 244 is configured to be connected to the buffer syringe 245. This is done. Needle 244 may be, for example, a blunt filling needle. A third set of Component 261 includes a vial 263 containing buffer, a cell filter 264, and a set of skins. Skin cell syringe 265, set of needles 266, unfiltered suspension syringe 267, set of spray nozzles 2 Includes 68 and a pair of scalpels 269. Buffer vial 263 contains, for example, 30 ml. It may also contain other suitable volumes of buffer. The buffer dilutes or neutralizes the enzyme. It may contain a suitable solution for doing so. For example, in some embodiments, The solution is physiological saline (for example, Ringer's lactate solution, sodium lactate solution, or HART). It may contain (man fluid). For example, one set of skin cell syringes 265 contains four skin cells It may include a suitable number of syringes, such as injection devices (for example, 10 ml syringes). The needle 266 consists of four needles (for example, stacked 2x2 in the third housing portion 260). It may include a suitable number of needles, such as a blunt-pointed filling needle. One set of spray nozzles 2 68 may include a suitable number of spray nozzles, such as four spray nozzles. A set of needles 26 Each of the 6 needles may be attached to one of the set of skin cell injectors 265. As a replacement for each needle in a set of needles 266, each spray nozzle in a set of spray nozzles 268 is a set of It may be attached to one of the skin cell injectors 265. First set of components T231, a second set of components 241, and a third set of components 26 Each component of 1 is the first set of components described above with respect to Kit 100 in Figure 1. The corresponding components of the second set of components and the third set of components. The components may be identical or similar in structure and / or function.

[0059] Figure 3 shows the housing of a kit for preparing a cell suspension as described herein. Devices packaged in a housing, etc., and devices packaged in a housing are used for detailed This is a flowchart showing method 300 for preparing a cell suspension. The kit is as described above. Any of the kits described herein, such as Kit 100 and / or Kit 200. That's good too.

[0060] Method 300 involves a first label and housing positioned adjacent to the first container of the apparatus. Identifying a first matching with a first visual indicator included in the housing portion of 1 Including (302). In response to (or after) the identification of the first matching, the first housing part Take a first set of components from and use the first set of components to The first part of the tissue processing method associated with container 1 may be performed (30 4) Also, the second label and the second housing of the device are located adjacent to the second container of the device. To identify a second match with a second visual indicator included in the housing portion. (306) In response to (or after) the identification of the second matching, the second housing Take a second set of components from the part, and use the second set of components, The second part of the tissue processing method associated with the second container may be performed (30 8).

[0061] In this method, one or more housing units are moved to the sterile surgical area. It may be as follows. In some embodiments, in response to the identification of the first match ( Alternatively, (after identification), the first housing part may move to the sterile surgical area. i. In some embodiments, in response to (or after) the identification of the second match, The second housing section may be designed to move into the sterile surgical area. In this embodiment, a third label and housing are arranged adjacent to the third container of the apparatus. To identify a third matching with a third visual indicator included in the third housing portion. This may also be done. A third label and a third housing are positioned adjacent to the third container of the apparatus. In response to the identification of a third matching with a third visual indicator included in the housing portion, Remove the third set of components from the third housing section, and the third set of components Using Nent, perform the third part of the tissue processing method associated with the third container. This may also be done. In some embodiments, as described above, the preparation tray of the device is used with respect to the device. The tissue manipulation unit of the tissue processing method may be executed. The first set of components includes a vial of sterile water, a syringe, and an enzyme vial. You can stay like that.

[0062] The first part of the tissue processing method associated with the first container is to prepare an enzyme mixture. The method may also include delivering the enzyme mixture to a first container. In this case, the enzyme vial is removed from the sterile pouch and placed in the first housing section as specified. The enzyme vial may be placed in the recessed area. In some embodiments, A set of components in 2 may include a buffer vial and a syringe. The second part of the tissue processing method associated with the container is to use a buffer of a certain volume The process involves drawing the liquid from the syringe into a syringe and delivering a certain volume of buffer from the syringe into a second container. This may include doing and . In some embodiments, a third set of components The Nent includes a syringe. The third part of the tissue processing method associated with the third container is skin The process involves decomposing skin samples and generating a cell suspension containing cells from the decomposed skin samples. This may include drawing the cell suspension into a syringe.

[0063] Exemplary Embodiments Figures 4-6 show the perspective view, top view, and bottom view of Kit 400, respectively. 400 is composed of any of the kits described herein, such as Kit 100 or Kit 200. The structure and / or function may be identical or similar. Kit 400 is a cell suspension. It may be used for preparation. Kit 400 includes housing 120 and The structure and / or function of any of the housings described herein, such as housing 220. It comprises a housing 420 which may be identical or similar. As shown in Figure 4, the housing 420 includes a base 470 and a lid 472 coupled to the base 470, It defines a physically sealed space and maintains the sterile condition inside the base 470 until the use of kit 400. Maintain the state. The lid 472 is fastened with, for example, one or more fasteners (e.g., adhesive, machine). A fastener, snap fit, and / or similar fasteners are used to connect to the base 470. It may include a removablely coupled cover. In some embodiments, The lid 472 contains polymer, foil, etc. (for example, bonded to the base 470 by adhesive). It may include a cover such as a thin film. The lid 472 is transparent or translucent. The contents of the housing 420 may be visible. For example, the lid 472 is It may also contain Tyvek®. Or, in some embodiments, The lid 472 includes a hinged cover (similar to, for example, clamshell packaging). That's fine.

[0064] Figures 7 and 8 are perspective views of the housing 420 with the cover 472 removed from the base 470. This is a top view. As shown in Figures 7 and 8, the base 470 has a lid 452 inside. A third housing section 460 having an integrated tray section 450 and a lid 471 is provided. In some embodiments, the lid 452 and / or lid 471 are made of the same material as lid 472. And the functions may be similar. Figure 9 shows the first by removing the covers 452 and 471. The housing portion 430, the second housing portion 440, and the third housing portion 460 are This is a top view of the exposed housing 420. As shown in Figure 9, the integrated tray section 450 The first housing section 430, the second housing section 440, and the device 410 are arranged therein. It is. Part or all of the integrated tray section 450 (for example, the first housing section 43 The liner 436 is coupled to the 0 and / or second housing portion 440) (For example, in the first housing portion 430 and / or the second housing portion 440) (and a cover to firmly hold the first and / or second set of components) They may be provided. In some embodiments, the first housing portion 430 and the second The housing portion 440 is a separate, different liner (for example, the first housing portion 430 A first liner coupled to the second housing portion 440 and a second liner coupled to the second housing portion 440 -) may have. Similarly, the liner 476 is coupled to the third housing portion 460. By doing so, the third housing portion 460 holds a third set of components. A cover may be provided for this purpose. Liner 436 and / or Liner 476 For example, it has an outer circumference that matches the shape of one or more components in the housing. It may include one or more cavities or recesses. In some embodiments, In this case, one or both liners are made of polyethylene terephthalate glycol (PET It may also contain suitable polymers such as G) or other suitable materials.

[0065] Figure 10 shows the removal of the liner 436 from the integrated tray section 450, and the third housing section. A perspective view of housing 420 with liner 476 removed from 460. The ing portion 430 and the second housing portion 440 are formed integrally with each other, forming a single unit. A structure (for example, a tray) may be formed. In some embodiments, the first The second housing section and the second housing section can be removed from each other as described above (for example, via perforations). They may be coupled in a manner that allows for connection. As shown in Figure 10, the first housing portion 430 is the Includes 1 visual indicator 432 (for example, the letter "A"), and second housing portion 4 40 includes a second visual indicator 442 (for example, the letter "B"), and a third how The zing section 460 includes a third visual indicator 462 (for example, the letter "C"). These visual indicators may be molded into the housing or as decals. It may be applied to the surface as a suitable marking such as CLS, or in any suitable manner. They may be located in the housing. Furthermore, these visual indicators are described below. The labels on the exterior of the device may match or correspond to the labels within each housing section. The components placed in the diagram may be associated with each part of the organizational processing method.

[0066] The first housing portion 430 is configured to receive the first set of components 431. It includes one or more recesses formed. For example, the first set of components 431 is a vial 433 containing a certain volume of water (for example, sterile water), and a needle 434 (shown in Figure 19). , and an enzyme syringe 435. The first housing 430 contains a vial containing the enzyme. Defines a vial recess 437 configured to receive. For example, an enzyme syringe 435 This may be a 10 ml syringe. The needle 434 is to be attached to the enzyme syringe 435. It is composed of the following. However, the first set of components 431 is suitable for the tissue processing method. It may include any suitable component. The recess is a snap-fit ​​(even The recess is sized to a suitable tolerance to hold a component that can be moved with a suitable force. (may be fixed and molded), and / or one or more fasteners (for example) (including stretchable materials, straps, extension mechanisms, covers, etc.), and / or similar items. It may be configured to receive a first set of components 431.

[0067] The second housing portion 440 is configured to receive a second set of components 441. It includes one or more recesses formed. The second set of components 441 is a buffer The system includes vial 443 containing, needle 444, and buffer syringe 445. Needle 444 contains It is configured to be attached to the syringe 445. The needle 444 is, for example, pointed. A filling needle may not be present. However, the second set of components 441 is a tissue processing method. It may include any suitable components that comply with the law. First housing section 430 Similar to the recess of the first part, the recess of the second housing part 440 is a snap-fit, one or Multiple fasteners, and / or similar, constitute a second set of components It may be configured to accept 441.

[0068] As shown in Figure 10, the integrated tray 450 is (for example, more detailed herein) (As explained below) it further includes a device recess configured to receive the device 410. Good. The recess in the device is a snap fit and / or one or more fasteners (for example) For example, stretchable materials, straps, extension mechanisms, covers, etc., and / or similar items. The similar parts may be sized and molded to suitable tolerances to hold the device. .

[0069] The third housing portion 460 is configured to receive a third set of components 461. It includes one or more recesses formed. A third set of components 461 is as described above. Vial 463 containing eel buffer, cell filter 464, set of skin cell syringes 465, A set of needles 466, an unfiltered suspension syringe 467, a set of spray nozzles 468, and a pair of meter Includes S469. Buffer vial 463 contains, for example, 30 ml of buffer. This is also good. One set of skin cell syringes 465 contains four skin cell syringes (for example, 10 ml each). It may include any suitable number of syringes, etc. One set of needles 466 consists of four needles. (For example, in the third housing section 460, the blunt fillings are stacked in a 2x2 pattern.) It may include any suitable number of needles, such as filling needles. A set of spray nozzles 468 has four It may include any number of suitable spray nozzles, such as spray nozzles. Each of the needles in a set of needles 466 It may be attached to one of a set of skin cell injectors 465. Also, a set of needles Each of the 466 needles is replaced by a set of spray nozzles 468, each spray nozzle is a set of skin cells It may be attached to one of the syringes 465. However, a third set of components T461 may include any suitable components suitable for the tissue processing method. Similar to the recess in housing portion 430 of the first housing portion, the recess in the third housing portion 460 is a snap Fit, one or more fasteners, and / or similar, by third It may be configured to accept a set of components 461.

[0070] The integrated tray section 450 and the third housing section 460 are removable from the base section 470. It is possible. For example, Figure 11 shows the integrated tray section 450 after it has been removed from the base section 470. Perspective view. Device 410, first housing including a first set of components 431. The second housing section 440, which includes section 430 and a second set of components 441, The figure shows the integrated tray section 450 in which it is installed. Figure 12 shows the base section 470 being removed. Then, in a perspective view of the third housing section 460 including a third set of components 461 Yes. Figure 13 shows a perspective view of the other components of kit 400 and a separate base 470. Yes. As shown in the figure, the base portion 470 is configured to receive the integrated tray portion 450. The first concave area 470a and the third housing portion 460 are arranged to be nested together. The second concave area 470b is defined.

[0071] Figure 17 shows a portion including a base 470 which includes a third housing portion 460 with a liner 476. This is a top view of the integrated tray section 450 removed from the disassemblable housing and base 470. For example, the integrated tray section 450 is lifted from the base section 470 and moved to the work area. This may help in the execution of the first and second parts of the organizational processing method.

[0072] Figure 18 shows the first housing section 430, the second housing section 440, and the device 410. A partially disassembled housing including an integrated tray section 450 and a first housing section 43 Top view of the liner 436 after it has been removed and separated from the 0 and second housing portion 440. Figure 19 shows the first set of components 431 and the second set of components. The integrated tray is shown with the 443 removed and not shown, and the 410 removed and separated. This is a top view of section 450.

[0073] Figure 23 includes a base 470, a third housing portion 460 and its liner 476 This is a top view of the partially disassembled housing, separated from the base 470. For example, The third housing section 460 is lifted from the base section 470 and moved to the work area. This may help in carrying out the third part of the tissue processing method. Figure 24 shows liner 476 removed. This is a top view of the third housing section 460.

[0074] Figure 14 is a top view of the device 410 with the cover removed. As shown in the figure, the device 4 10 comprises a preparation tray 417 disposed in the concave area 458 of the apparatus 410. Furthermore, the apparatus 410 contains the first container 411, the second container 413, and the third container 415. It is specified that the apparatus 410 also has a first label 412, a second label 414, and a third label 412. Label 416 (for example, a colored labeled ring placed around the container) The first label 412 includes (for example, a common color and / or the letter "A") (Therefore) it may correspond to the first visual indicator 432 and the second label 41 4 is a second visual (for example, by including a common color and / or the letter "B") It may correspond to indicator 442, and the third label 416 is the third visual indicator It may also correspond to Cater 462 (for example, the letter "C"). Figure 16 shows the enzyme vial. A perspective view of an exemplary embodiment of 482. In some embodiments, the first container 411 may be configured to receive enzyme vial 482. Several implementations In this embodiment, the first container 411 receives the contents of the enzyme vial 482 via a syringe. It may be configured to accommodate.

[0075] The preparation tray 417 may have a bottom surface surrounded by side walls. Part or all of the bottom surface may be skin It may constitute a tissue manipulation area. The preparation tray 417 is on both sides of the preparation tray 417. It may include one or more collection areas 409, such as two collection areas on the side. Area 409 is where the fluid injected into the preparation tray 417 flows into the collection area 409 and / or the surface of the bottom of the preparation tray 417 is concave relative to the rest of the surface so that it can be stored. It may be. Figure 15 shows the apparatus in which the preparation tray 417 is removed from the concave area 458. This is a top view of 410. Other exemplary features of the preparation tray 417 are described below in more detail. I will explain in detail.

[0076] Figure 20 is a diagram of a part of the apparatus 410. As shown in the figure, the first label 412 is the first The second label 414 surrounds the second container 413, and the third label 416 surrounds the second container 411. The third container 415 is surrounded by the apparatus 410 as described above with respect to the kit 100 in Figure 1. There are various buttons, such as button 419 which can be pressed to activate the heating assembly. It may also be equipped with a set of one or more heated assembly elements. It may include a dicater 418. One or more heating assembly indicators 41 8, as described above with respect to kit 100 in Figure 1, and the indicator light of device 410. The association determines the status of one or more devices (for example, the heating status of device 410). Includes an indicator configured to show one or more statuses of the semblage. It may be. Figure 21 shows the part of the apparatus 410 shown in Figure 20 (for example, manipulating tissue). This shows the device 410 (next to the preparation tray).

[0077] Figure 22 shows the first set of components 430, the enzyme syringe 435 and the first container 411 The intended relationship and the second set of components 440 buffer syringe 445 and A perspective view of the apparatus 410, including an enlarged portion to show the intended relationship with container 413. be.

[0078] Any of the devices described herein (for example, device 110, device 210, and device 4) 10) The preparation tray receives fluid from the preparation tray (for example, via the needle of a syringe). The base may have any suitable shape and contour so that the collection can be effective. For example, Figures 25 and 26 are perspective and cross-sectional views of the preparation tray 517, respectively. The preparation tray 517 has a bottom surface 553 whose outer circumference is surrounded by side walls 554. The bottom surface 553 is It includes surface 557, a first lower surface 555A, and a second lower surface 555B. The main surface 557 is It may be a skin tissue manipulation area, or it may include a skin tissue manipulation area. Also, The bottom surface 553 is a first surface that is tapered downward from the main surface 557 to the first lower surface 555A It includes the transition surface 556A. Also, the bottom surface 553 extends from the main surface 557 to the second lower surface 555B. It includes a second transition surface 556B that is tapered downward. As shown in Figure 26, the first and The second transition surface is shown as having a slope along a straight line. However, some implementations In terms of form, this transition may be curved (for example, convex, concave, etc.) or arbitrary It is understood that it may have a suitable shape. Furthermore, several implementation forms In this state, the transition surfaces 556A and 556B are located at each corner or edge of the preparation tray 517. It may extend in this way (for example, the lower surfaces 555A and 555B may be omitted). The first lower surface 555A and / or the first transition surface 556A integrally form the first collection area A is defined, and the second lower surface 555B and / or the second transition surface 556B are integrally the second By defining the collection area, the first collection area and the second collection area are defined from the main surface 557. The fluid may be able to flow to one or both of the collection areas. The first and second collection areas may be located at the opposite end of the preparation tray 517. (For example, it helps to facilitate easy organizational collection for both left-handed and right-handed users.) (Standing). In some embodiments, the preparation tray is added or replaced as shown in Figure 25. Using the same transition surface and lower surface as shown in Figure 26, the front of the preparation tray 517 The region and / or rear region may include a collection area. The preparation tray 517 is actually Although it is shown as having a qualitative semicircular or crescent shape, any suitable shape is shown ( For example, they may be egg-shaped, rectangular, or circular.

[0079] Figures 27 and 28 are perspective and cross-sectional views of the preparation tray 617, respectively. The tray 617 has a bottom surface 653 whose outer perimeter is surrounded by side walls 654. The bottom surface 653 is the main surface 6 57, the lower surface 655A, and the main surface 657 to the first lower surface 655A are tapered downwards. It includes an inclined transition surface 656A. As shown in Figure 28, the transition surface 656A is roughly on a straight line. It is shown as having a slope. However, in some embodiments, this transition It may be curved (for example, convex, concave, etc.) or have any preferred shape. It is understood that this is also acceptable. Furthermore, in some embodiments, the transition surface 65 6A may extend to the corners or edges of the preparation tray 617 (for example, the lower surface 6 (55A may be omitted). The main surface 657 may be a skin tissue manipulation area. It may include a skin tissue manipulation area. Lower surface 655A and / or transition surface 656A By integrally defining the collection area, fluid flows from the main surface 657 to the collection area. It may be possible to do so. In Figures 27 and 28, the lower surface 655A is prepared Although shown as being located on the left side of the tray, in some embodiments, It is understood that a similar low-profile surface 655A may be positioned mirror-image on the right side of the tray. It shall be so. Furthermore, in some embodiments, the preparation tray 617 may be additional or Alternatively, the same transition and lower surfaces as shown in Figures 27 and 28 can be used to adjust the adjustment. The preparation tray may include collection areas in the front and / or rear regions. I-617 is shown as having a substantially semicircular or crescent shape, but any It may also be in a preferred shape (for example, egg-shaped, rectangular, or circular).

[0080] Figures 29 and 30 are perspective and cross-sectional views of the preparation tray 717, respectively. Tray 717 is described above with respect to Figures 25 and 26, except that the main surface 557 is omitted. The preparation tray 717 may be similar to the preparation tray 517. The outer circumference of the bottom surface 753 is the side wall. It is surrounded by 754. The bottom surface 753 is the first lower surface 755A and the second lower surface 755 Includes B. Also, the bottom surface 753 extends from the center line of the preparation tray toward the first lower surface 755A. It includes a first transition surface 756A that is tapered downward. The bottom surface 753 is a preparation tray From the center line of (i) (for example, the upper edge of the first transition surface 756) toward the second lower surface 755B It includes a second transition surface 756B that was previously tapered downwards. Alternatively, it includes a first transition surface 7 56A and / or the second transition surface 756B have an upper edge that coincides with the center line of the preparation tray. It is not necessary for each upper edge to be offset from the center line of the preparation tray (for example) (By tilting, lateral displacement, etc.), the first transition surface 756A or the second transition surface 756 B may be larger than the other transition surface. Also, in some embodiments, One or both transition surfaces may be curved (e.g., convex, concave, etc.), or any other preference. It is understood that it may have a suitable shape. Furthermore, several embodiments In this configuration, one or both transition surfaces extend to the corner or edge of the preparation tray 717. (For example, the lower surfaces 755A and / or 75B may be omitted.) The first transition surface 756A and the second transition surface 756B were in the skin tissue manipulation area. It may also include a skin tissue manipulation area. The first lower surface 755A and / or Alternatively, the first transition surface 756A integrally defines the first collection area, and the second lower surface 755B and / or the second transition surface 756B integrally defines the second collection area. This allows fluid to flow to one or both of the first and second collection areas. It may be as follows: The first collection area and the second collection area are of the preparation tray 717. It may be located at the opposite end. Furthermore, in some embodiments, a preparation tray (i) is an additional or alternative transition surface and lower surface similar to those shown in Figures 29 and 30. Using this, the collection area is included in the front and / or rear regions of the preparation tray 717. The preparation tray 717 may be substantially semicircular or crescent-shaped. Although shown, any suitable shape (for example, egg-shaped, rectangular, or circular) is also acceptable. stomach.

[0081] Figures 25 to 30 show at least one collection area on the side (for example, left side, right side). While exemplary embodiments of the preparation tray are shown, in some embodiments, Even if one or more collection areas are located in other areas of the tray, the tray is still manufactured. Good. For example, Figures 31 and 32 are perspective and cross-sectional views of the preparation tray 817, respectively. This is a diagram showing that the preparation tray 817 is a collection tray located in the central area of ​​the preparation tray 817. Includes area. The preparation tray 817 has a bottom surface 853 whose outer circumference is surrounded by side walls 854. bottom Surface 853 tapers from the first end of the preparation tray 817 toward the center of the preparation tray 817. The first transition surface 856A is inclined downward in a curved shape, and the preparation tray 817 extends from the second end to the preparation tray Tapered toward the center of Ray 817 (for example, the lower edge of the first transition surface 856A) Includes a downwardly inclined second transition surface 856B, or a first transition surface 856A and / or the second transition surface 856B may have a lower edge that does not coincide with the center line of the preparation tray. Often, each lower edge is offset from the center line of the preparation tray (for example, inclined, lateral). By displacement, etc., the first transition surface 856A or the second transition surface 856B moves toward the other transition surface. It may be larger than the transition plane. Also, in some embodiments, one or both The transition surface may be curved (for example, convex, concave, etc.) or may have any preferred shape. It shall be understood that this is permissible. First transition surface 856A and second transition surface Each of 856B may be a skin tissue manipulation area or may include a skin tissue manipulation area. It may be so. The first transition surface 856A and the second transition surface 856B form a single collection area. By defining this, the intersection of the first transition surface 856A and the second transition surface 756B is directed toward It may be possible for a fluid to flow through it in the past. Furthermore, in some embodiments, The preparation tray may have transition surfaces similar to those shown in Figures 31 and 32, either as an addition or replacement. Using the lower surface, collect the collection area in the front and / or rear regions of the preparation tray 817. It may contain A. The preparation tray 717 has a substantially semicircular or crescent shape. Although shown as a single shape, it can be any preferred shape (e.g., egg-shaped, rectangular, circular). That's fine.

[0082] Figures 33 and 34 are perspective and cross-sectional views of the preparation tray 917, respectively. The tray 917 has a bottom surface 953 whose outer perimeter is surrounded by side walls 954. The bottom surface 953 is the main surface 9 It includes surface 57, lower surface 955B, and transition surface 956B. The main surface 957 is a skin tissue manipulation area. It may be A, or it may include a skin tissue manipulation area. The transition surface 956A is the main surface From 957 to the lower surface 955A, it slopes downward in a tapered manner. As shown in Figure 34, transition Surface 956B is shown as having an inclination that is roughly straight. However, some actual In the application form, this transition may be curved (for example, convex, concave, etc.), or arbitrary. It shall be understood that the lower surface 955A and the transition The transition surface 956A integrally defines the collection area, thereby the first collection from the main surface 957 The area may be configured to allow fluid to flow into it. The collection area is the preparation tray 917. They may be located at the ends and / or corners. Preparation tray shown in Figures 33 and 34. The collection area is located to the right of the preparation tray 917, but in some embodiments, It may also be present on the left side (or front or back, etc.) of the preparation tray. I-917 indicates that it has a substantially semicircular or crescent shape, but any It may also be in a preferred shape (for example, egg-shaped, rectangular, or circular).

[0083] Figures 35 and 36 are perspective and cross-sectional views of the preparation tray 1017, respectively. The tray 1017 has a bottom surface 1053 whose outer perimeter is surrounded by side walls 1054. This includes the lower surface 1055A and the second end of the preparation tray 1017 from the first end of the bottom surface 1053. A transition surface 1056 that is tapered downwards to the first lower surface 1055A located at the end of the A and, including, as shown in Figure 36, the transition surface 1056A has a slope that is roughly straight. It is shown as such. However, in some embodiments, this transition is curved (even It is understood that the shape may be convex, concave, etc., or may have any suitable shape. It shall be so. The transition surface 1056A may be a skin tissue manipulation area, or a skin tissue The weaving operation area may be included. The lower surface 1055A and / or the transition surface 1056A are By defining the collection area as a single unit, fluids can flow towards the collection area. It may be so. The collection area of ​​the preparation tray 1017 shown in Figures 35 and 36 is for preparation It is located on the left side of the tray, but in some embodiments, it is located on the left side (or front) of the preparation tray. They may be present similarly on the side or rear side, etc. The preparation tray 1017 is substantially semicircular. Although shown as having a shape or a crescent shape, any suitable shape (for example, egg) The shape may be rectangular or circular.

[0084] In addition or alternatively, in some embodiments, there is at least one preparation tray. The skin sample may include at least one textured surface. To make the surface more easily disassembled by friction, pressure, or force, The textured surface may be placed in the skin tissue handling area of ​​the preparation tray. In the applied form, at least one textured surface includes one or more raised features. It may be so. For example, one or more raised features may be approximately 0.05 mm to approximately 2mm, approximately 0.05mm to approximately 1.5mm, approximately 0.05mm to approximately 1mm, Approximately 0.1mm to approximately 2mm, approximately 0.1mm to approximately 1.5mm, or approximately It may have a height of 0.1 mm to approximately 1 mm. In some embodiments, At least one textured surface may have one or more concave features as an addition or replacement. It may include. For example, one or more concave shapes may be approximately 0.05 mm to Approximately 2mm, approximately 0.05mm to approximately 1.5mm, approximately 0.05mm to approximately 1mm m, approximately 0.1mm to approximately 2mm, approximately 0.1mm to approximately 1.5mm, or It may have a depth of approximately 0.1 mm to about 1 mm. In some embodiments, , one or more raised features and / or one or more concave features, approximately 0.05mm to approximately 1mm, approximately 0.05mm to approximately 0.5mm, or approximately 0 It may have a width or depth of 0.5 mm to approximately 1 mm.

[0085] For example, as shown in Figure 37, the skin tissue manipulation area 1151 is arranged in a two-dimensional array. It may include one or more raised texture features of a set. At least one of them is columnar (for example, circular cross-section, other polygonal cross-section), and / or, at least one of the raised shapes is tapered (e.g., pyramidal, doveyed) It is conceivable that it may have a shape such as a m-shape or any other suitable form. For example, several In the embodiment, part or all of the raised shape is tapered and has an uppermost point. It may be. Such tapered shapes have the same height as described above in some respects. and / or width. A set of raised features may be rectangular as shown in Figure 37. Ray, or triangular array, hexagonal array, irregular or random array, etc., any preferred They may be arranged in an appropriate manner. Similarly, in some embodiments, the preparation tray is In addition or as an alternative, a rectangular array similar to that shown in Figure 37 and / or any It may include a set of concave features (e.g., depressions) arranged in a suitable array. Figure 3 7, for illustrative purposes, shows two collection areas similar to those described above with respect to Figures 25 and 26. The figure shows a textured surface on a preparation tray having any preparation tray (for example, The preparation trays described above in Figures 25-36 also have the same protrusions as described above in Figure 37. It may include at least one textured surface having a shape and / or a recess. This shall be understood.

[0086] As another example of a preparation tray with a textured surface, Figure 38 shows a tray arranged in a one-dimensional array. A prepared skin manipulation area 1251 including one or more raised texture shapes This is a perspective view of Ray 1217. For example, as shown in Figure 38, the skin tissue manipulation area is It contains one or more elongated ridges (for example, parallel ridges) It is also fine. In some embodiments, one or more elongated ridges are roughly prismatic in shape. For example, it could be a rectangular prism shape, or it could be angled (for example, a slanted fin). It is fine, and it may also be dome-shaped (for example, with a curved cross-sectional profile). Elongated ridge They may be arranged parallel to each other, or in any preferred manner (for example, at an angle or The arrangement may be non-parallel, random, etc. Similarly, in some embodiments, the preparation Ray may, as an addition or alternative, be in a manner similar to that shown in Figure 38 and / or any other preference. It may include a set of concave features (e.g., trenches) arranged in a suitable array. Furthermore, Figure 38 shows two examples similar to those described above with respect to Figures 25 and 26. The image shows a textured surface on a preparation tray having a collection area, but any preparation tray ( For example, the preparation trays mentioned above in Figures 25 to 36, and the one mentioned above in Figure 38. Includes at least one textured surface having similar raised and / or recessed features. It shall be understood that obtaining it is permitted.

[0087] The transition surfaces of the preparation tray described herein allow the flow of cells through one or more preparation trays. To guide towards a suitable collection area, it has any suitable taper or inclination angle. This may also be the case. In some embodiments, the inclination or slope of the preparation tray is approximately 0.5 °~approximately 35°, approximately 0.5°~approximately 25°, approximately 0.5°~approximately 15°, Approximately 5° to 35°, approximately 5° to 25°, approximately 5° to 15°, approximately It can be 15° to approximately 35°, or approximately 15° to approximately 25°. In the embodiment, a preferred gradient is, for example, the properties of the material (e.g., hydrophobicity) and / Alternatively, it may vary depending on factors such as the surface coating of the preparation tray. For example, highly hydrophobic materials and A preparation tray with a surface coating guides the flow of cells toward the collection area. It may have a sufficiently shallow transition surface. For example, Figure 39 shows the cross section of the preparation tray 1317. This is a part of the view drawing. Preparation tray 1317 is used for preparation tray 517 and other preparations as described herein. One of the trays may have the same or similar structure and / or function. The preparation tray 1317 has a main surface 1357, a lower surface 1355B, and a lower surface from the main surface 1357. It includes a tapered transition surface 1356B up to surface 1355B. The transition surface 1356B is the main surface 1 From 357 to the lower surface 1355B, it is tapered at a 4° angle with respect to the main surface 1357. Yes, they are.

[0088] Figure 40 is a partial cross-sectional view of the preparation tray 1417. The preparation tray 1417 is a preparation tray Ray 517, etc., is identical in structure and / or function to any of the preparation trays described herein. Or similar. For example, the preparation tray 1417 has a main surface 1457 and a lower surface 1455B, and a tapered transition surface 1456B from the main surface 1457 to the lower surface 1455B, This includes the transition surface 1456B from the main surface 1457 to the lower surface 1455B, and the main surface 1457 In contrast, it has a tapered shape at a 4° angle.

[0089] Figure 41 is a partial cross-sectional view of the preparation tray 1517. The preparation tray 1517 is a preparation tray Ray 517, etc., is identical in structure and / or function to any of the preparation trays described herein. Or similar. For example, the preparation tray 1517 has a main surface 1557 and a lower surface 1 Includes 555B, a first transition surface 1556B, and a second transition surface 1556C. First transition Surface 1556B is tapered at a first angle from the main surface 1557 to the second transition surface 1556C. This may be the case. The second transition surface 1556C is connected from the first transition surface 1556B to the bottom surface 15 Up to 55B, it may be tapered at a second angle. The first transition surface 1556B is the main From surface 1557 to the second transition surface 1556C, the surface is tapered at an angle of 4° relative to the main surface 1557. The second transition surface 1556C is located between the first transition surface 1556B and the lower surface 155 Up to 5B, the surface is tapered at a 7° angle relative to the main surface 1557.

[0090] Figure 59 is a top view of apparatus 2010 for generating cell suspension. Apparatus 2010 is , any of the devices described herein that are identical or similar in structure and / or function This is also acceptable. For example, the apparatus 2010 is equipped with a preparation tray 2017. 7 has a bottom surface 2053 surrounded by side walls 2054. The bottom surface 2053 is the preparation tray 201 A recess or cavity 2099 (for example, a circular collection area) located in the corner of 7 By including (for example, if the opposite end of the preparation tray 2017 is lifted) The fluid may collect in this recess or cavity 2099. When stored in the cavity 2099, for example, as described in detail above, a syringe Bulk retrieval of fluids using methods such as these can become more efficient. In the application configuration, one of the above-mentioned preparation trays has a similar recess or cavity in the collection area. It may contain ti.

[0091] Figure 42 is a perspective view of an exemplary embodiment of Kit 1600. Kit 1600 is Any of the kits described herein is identical or similar in structure and / or function. This is also fine. For example, kit 1600 includes a first housing part 1630, a second housing The housing 1620 comprises a section 1640 and a third housing section 1660. The first housing portion 1630 is a tissue treatment method, such as any tissue treatment method described herein. It is configured to receive a first set of components 1631 associated with the first part. The formed recess is included. The second housing portion 1640 is associated with the second part of the tissue processing method. Includes a recess configured to receive a second set of attached components 1641 The third housing portion 1660 is a third part associated with the third part of the tissue processing method. Includes a recess configured to receive the set of components 1661. First housing Part 1630 is a first visual indicator 1632 associated with a first label of the device. This includes. The apparatus is identical in structure and / or function to any of the apparatus described herein. The same may apply. The second housing portion 1640 is associated with the second label of the device. The device includes a second visual indicator 1642. The third housing portion 1660 is the device Includes a third visual indicator 1662 associated with a third label.

[0092] As shown in Figure 42, the first housing portion 1630, the second housing portion 1640, The third housing section 1660 is formed integrally with each other, forming a single structure (for example For example, it constitutes a tray. As shown in Figure 42, the first visual indicator 163 2 contains the letter "A", the second visual indicator 1642 contains the letter "B", and the third Visual indicator 1662 contains the letter "C". Other types of visual indicators are available as additions or alternatives. Visual indicators (e.g., characters, symbols, punctuation, textures, etc.) are included in the housing. It may be made transparent. For example, part of the housing 1620 may be transparent, and the first The visual indicator 1632 is a blue back visible through the top of the housing 1620. The second visual indicator 1642 may include a view, and is located above the housing 1620. It may include a gray background visible throughout the section, and a third visual indicator 166 2 may include a green background visible through the top of the housing. Also, see Figure 4. As shown in 2, the first set of components 1631, the second set of components 1 641, and each component of the third set of components 1661 is accepted One or more of the recesses or cavities configured to be each component It is possible to guide the user to use the components in the intended order (for example, take them out and implement them). To enable this functionality, it may be the same as the reference number for a specific step in the tissue processing method.

[0093] As shown in Figure 42, the first set of components 1631 contains a certain volume of water (even The vial 1633 containing sterile water, needle 1634, enzyme syringe 1635, and cell filter This includes the overflow syringe 1664. For example, the enzyme syringe 1635 may be a 10 ml syringe. The needle 1634 is configured to be attached to the enzyme syringe 1635. One set of components 1631 is optionally selected as the same enzyme vial 1682 as described above. Includes.

[0094] The second set of components 1641 includes a vial 1643 containing a buffer and a needle 164 4. Includes buffer syringe 1645 and unfiltered suspension syringe 1667. Needle 1644 is , configured to be coupled to buffer syringe 1645. Needle 1644 is, for example, A blunt filling needle may also be used. Furthermore, the second set of components 1641 is one It includes a pair of females 1669. Also, a second set of components 1641 is similar to those described above. It may include vial 1663 containing the buffer. Buffer vial 1663 is For example, it may contain 30 ml of buffer.

[0095] A third set of components 1661 includes a set of skin cell syringes 1665 and a set of needles 1 Includes 666 and a set of spray nozzles 1668. A set of skin cell injectors 1665 is 4 One skin cell syringe (for example, a 10 ml syringe), or any suitable number of skin cell injections. It may include a container. A set of needles 1666 consists of four needles (for example, a third housing part) Any suitable number of needles (such as blunt filling needles stacked in a 2x2 configuration in 1660) It may include any suitable spray nozzles, such as four spray nozzles, etc. A set of spray nozzles 1668 may include any suitable spray nozzles, etc. It may include several spray nozzles. Each of the 1666 needles in a set is part of a set of skin cell injectors. It may be combined with one of the 1665s. Also, each of the hands of a set of hands 1666 may be replaced and Then, each spray nozzle of a set of spray nozzles 1668 is connected to a set of skin cell syringes 1665. It may be attached to one of the elements.

[0096] Figure 43 is a perspective view of an exemplary embodiment of Kit 1700. Kit 1700 is Any of the kits described herein is identical or similar in structure and / or function. This is also fine. For example, kit 1700 includes a first housing part 1730, a second housing The housing 1720 comprises a section 1740 and a third housing section 1760. The first housing portion 1730 is a tissue treatment method, such as any tissue treatment method described herein. The first part is configured to receive a first set of components 1731 associated with the first part. The formed recess is included. The second housing portion 1740 is associated with the second part of the tissue processing method. Includes a recess configured to receive a second set of attached components 1741 The third housing portion 1760 is a third one associated with the third part of the tissue processing method. Includes a recess configured to receive the set of components 1761. First housing Part 1730 is a first visual indicator 1732 associated with a first label of the device. This includes. The apparatus is identical in structure and / or function to any of the apparatus described herein. The same may apply. The second housing portion 1740 is associated with the second label of the device. The device includes a second visual indicator 1742. The third housing portion 1760 is the device This includes a third visual indicator 1762 associated with a third label.

[0097] As shown in Figure 43, the first housing portion 1730, the second housing portion 1740, The third housing section 1760 is formed integrally with each other, forming a single structure (for example For example, it constitutes a tray. As shown in Figure 43, the first visual indicator 173 2 is a first that at least partially encloses each component of the first set of components It may also include a color label (for example, blue), and a second visual indicator 1742 This is a second color that at least partially surrounds each component of the second set of components. It may include a label (for example, gray), and the third visual indicator 1762 is , a third color that at least partially surrounds each component of the first set of components It may include a label (for example, green). Also, as shown in Figure 43, the first set Component 1631, a second set of components 1641, and a third set Each component 1661 has a recess configured to receive each component. One or more of the cavities allow the user to place each component in the order they intended. The specific organizational processing method is designed to guide users to use it (for example, extract and implement it). It may be identified by a reference number for a specific step.

[0098] As shown in Figure 43, the first set of components 1731 contains a certain volume of water (even The vial 1733 containing sterile water, needle 1734, enzyme syringe 1735, and cell filter This includes the overflow syringe 1764. For example, the enzyme syringe 1735 may be a 10 ml syringe. The needle 1734 is configured to be attached to the enzyme syringe 1735. One set of components 1731 optionally includes an enzyme vial 1782.

[0099] The second set of components 1741 includes a vial 1743 containing a buffer and a needle 174 4. Includes buffer syringe 1745 and unfiltered suspension syringe 1767. Needle 1744 is , configured to be coupled to buffer syringe 1745. Needle 1744 is, for example, A blunt filling needle may also be used. Furthermore, the second set of components 1741 is one It includes a pair of females 1769. Additionally, a second set of components 1741 includes a buffer. It may contain vial 1763. Buffer vial 1763 may contain, for example, 30 ml. It may include a buffer of l.

[0100] A third set of components 1761 includes a set of skin cell syringes 1765 and a set of needles 1 766, and a set of spray nozzles 1768. A set of skin cell injectors 1765, 4 One skin cell syringe (for example, a 10 ml syringe), or any suitable number of skin cell injections. It may include a container. A set of needles 1766 consists of four needles (for example, a third housing part) Any suitable number of needles (such as blunt filling needles stacked in a 2x2 configuration in 1760) It may include any suitable spray nozzles, such as four spray nozzles, etc. A set of spray nozzles 1768 may include any suitable spray nozzles. It may include several spray nozzles. Each of the 1766 needles in a set is part of a set of skin cell injectors. It may be combined with one of the 1765s. Also, each of the needles in a set of needles 1766 may be a replacement for one of the needles. Then, each spray nozzle of a set of spray nozzles 1768 is connected to a set of skin cell syringes 1765. It may be attached to one of the elements.

[0101] Figure 44 is a perspective view of an exemplary embodiment of Kit 1800. Kit 1800 is Any of the kits described herein is identical or similar in structure and / or function. This is also fine. For example, kit 1800 includes a first housing part 1830, a second housing It comprises a housing 1820 including a section 1840 and a third housing section 1860. The first housing portion 1830 is a tissue treatment method, such as any tissue treatment method described herein. The first part is configured to receive a first set of components 1831 associated with the first part. The formed recess is included. The second housing portion 1840 is associated with the second part of the tissue processing method. Includes a recess configured to receive a second set of attached components 1841 The third housing portion 1860 is a third one associated with the third part of the tissue processing method. It includes a recess configured to receive a set of components 1861.

[0102] Kit 1800 is identical in structure and / or function to any of the devices described herein. The kit 1800 comprises a first housing portion 18 30, a second housing portion 1840, and a configuration configured to receive the device 1810 It comprises an integrated tray 1850. First housing part 1830 and second housing part 1840 is an integrated tray configured to be lifted from the integrated tray 1850. It is formed as a structure.

[0103] Furthermore, kit 1800 comprises a base 1870 and a box 1880. Base 18 70 is configured to receive the integrated tray 1850 and the third housing portion 1860. The box 1808 has a base 1870 attached to the first part of the box 1808. The second part of the box 1808 is configured to receive the enzyme vial recess 188 Box 1808 is configured to receive enzyme vial 1882. Enzyme vial 1882 or enzyme vial 188 in the second part of box 1808 Regardless of the sterile condition of the pouch containing 2, the base of the first part of the box 1808 To maintain the sterile condition of the contents of 1870, the second part of the box 1808 The box 1808 is further provided with a partition 1859 arranged to separate the first part of the box 1808. It's okay to do so.

[0104] In some embodiments, the base 1870 is such that when the pull tab is pulled, the sealing portion breaks. Removable seal portion of base 1870 to allow access to the inside of base 1870 It may be formed as an integrated protective packaging layer including a pull tab attached to it. Furthermore, the base portion 1870, the integrated tray portion 1850, the first housing portion 1830, and the second housing portion The woving section 1840 and the third housing section 1860 are each part of kit 1800. It may be transparent so that the user can see the components installed inside.

[0105] Methods for packaging the kit Figures 45 to 58 show the kits described herein, etc., for shipping purposes. This shows the steps for packaging one set of kits. Figures 45 to 58 show three kits. This shows how to package a set into a single shipping container, but there are several implementations. In this state, this method can be used with any suitable number (for example, 1, 2, 4, 5, 6 or more) It is understood that the (high-quality) kit can be modified to be packaged in a shipping container. The method shown in Figures 45 to 58 is performed over a predetermined period of time, such as up to approximately 48 hours. So, the kit's stable temperature is set to the desired temperature or temperature range (for example, approximately 20°C to...) It may include stacking of cold chain shipping materials to maintain a temperature of approximately 25°C. Maintaining a stable temperature within a certain desired temperature range is, for example, one of the kits. Or impair the viability or chemical state of multiple components (e.g., enzymes) It is considered important because it does not exist.

[0106] Generally, the method of packaging a kit (any of the kits described herein) is , in the insulated container, one or more kits between the foam insert and the refrigerated brick material This may include stacking the containers. The containers may, for example, be made of insulating material, at least in part. (For example, insulating foam) and / or one or more It may be insulated by having an insulating liner or pad. Packaging Absorbent wipes that help absorb condensation, etc., may be placed within the layer of materials.

[0107] For example, Figure 45 shows an empty container 1990. Container 1990 is, for example, For example, it may be formed from any suitable material such as cardboard or foam. Container 1990 , including insulation 1988 and liner 1989. As shown in Figure 46, the first form Insert 1991 may be placed in container 1990. Insert 1991 provides a set of refrigerated bricks 1992 (for example, refrigerated FB24 bricks). A central opening that can be installed may be specified. For example, a set of five refrigerated bricks 1992 It may also be installed in the central opening.

[0108] As shown in Figure 47, a set of absorbent wipes 1993 (for example, two absorbent wipes) It may also be placed on top of the refrigerated bricks 1992.

[0109] As shown in Figure 48, the second foam insert 1994 is absorbent of the container 1990. It may also be placed on top of Wipe 1993.

[0110] As shown in Figure 49, the first kit 1902A is placed in the second form of container 1990. It may also be installed on top of the 1994.

[0111] As shown in Figure 50, the second kit 1902B is placed in container 1990 of the first kit 19 It may also be installed on top of 02A.

[0112] As shown in Figure 51, the third kit 1902C is placed in container 1990, and the second kit 19 It may also be placed on top of 02B. Similarly, the additional kit can be placed on top of the third kit. It may be possible to install them in a designated area.

[0113] As shown in Figure 52, the third form insert 1995 is placed in the third container 1990. It may also be placed on top of Kit 1902C.

[0114] As shown in Figure 53, absorbent wipes 1996 (for example, two absorbent wipes) are placed in the second It may be placed on top of the form insert 1995.

[0115] As shown in Figure 54, the fourth foam insert 1997 is absorbent of the container 1990. It may be placed on top of the wipe 1996. The fourth form insert 1997 is A central opening is provided in which a set of refrigerated bricks 1998 (for example, refrigerated FB24 bricks) can be placed. It may be specified. For example, a set of five refrigerated bricks 1998 are placed in the central opening. You may do so.

[0116] As shown in Figure 55, the insulation pad 1999 (for example, insulation foam) is placed in container 19 The fourth foam insert of 90 may be placed on top of the 1997 insulation pad. 1999, for example, to create a sealed space that captures the thermal energy inside a container. It can be useful. In some embodiments, the insulation pad 1999 can be attached to the wall of the container. By ensuring close contact, the gap between the insulation pad 1999 and the container wall or The size may be specified to reduce the amount of heat energy escaping through space. Alternatively, the insulation pad may be wrapped in (for example, backed with) metal foil. By including a composting element, the heat energy inside the container is retained for as long as possible. It could be useful for others.

[0117] As shown in Figure 56, the liner 1989 defines a closed internal space within the container 1990. To determine, it may be closed. Liner 1989 states, for example, packaging container It functions to help bind the components together tightly (snugly) and the package You may also want to suppress undesirable relative axial runout of the jigging component. Alternatively, Liner 1989 has a container that deteriorates and endangers the kit inside. It helps protect surrounding containers (for example, cardboard boxes) from condensation, which can be a cause of condensation. It can function to stand upright. For example, Liner 1989 is suitable for water resistance such as polyethylene. Or it may include a waterproof layer (for example, a sheet, bag, or other cover). In the embodiment, the container (or other moisture-sensitive components) is protected from moisture such as condensation. To protect the extra or redundant liner (t), multiple (e.g., two or more) liner 1989 This may be included in container 1990.

[0118] As shown in Figure 57, by closing and securing the flap 1987 of container 1990 Alternatively, container 1990 may be sealed (for example, with tape).

[0119] As shown in Figure 58, container 1990 is placed in the chamber along with other containers. You may consider going on strike.

[0120] Various concepts can be implemented in one or more ways, and at least one of them An example was presented. The actions performed as part of this method can be ordered in any preferred manner. It is possible. Therefore, although it is shown as a continuous operation in the exemplary embodiment, The actions may be executed in a different order than shown in the example, including performing several actions simultaneously. Embodiments can be constructed. In other words, such features do not necessarily have to be specific. Not limited to row order, but rather, in a manner consistent with this disclosure, continuous, asynchronous , concurrently, simultaneously, synchronously, and / or any number that can be performed similarly. This could include threads, processes, services, servers, and / or similar entities. It shall be understood that some of the above features are in a single embodiment. They can contradict each other in that they may not exist simultaneously. Similarly, Some characteristics are applicable to one aspect of technological innovation, but not to other aspects. That is the case.

[0121] Furthermore, this disclosure may include other technological innovations not described herein. Persons have the right to implement such technological innovations, additional applications, continuation applications, partial continuation applications, and divisional applications. All rights relating to the technological innovation, including the application and / or similar rights. We reserve the right to express our opinion regarding the advantages, embodiments, examples, functions, features, logic, operation, and system of this disclosure. Structure, topology, and / or other aspects are defined in the embodiments of this disclosure. It is understood that this should not be considered a limitation or a limitation on an equivalent embodiment. This refers to the specific needs and / or characteristics of individual and / or corporate users, and databases. System configuration and / or relational model, data type, data transmission and / or network Depending on the work framework, syntactic structure, and / or similar thereto, this specification The technologies disclosed herein enable a great deal of flexibility and customization as described. Various embodiments of this can be realized.

[0122] All definitions used herein are those found in dictionaries or references. It is understood that this will control the ordinary meaning of the defined terms and / or other terms.

[0123] As used herein, in certain embodiments, the term "approximately" preceding a numerical value. "(about)" or "approximately" means within a range of ±10% of the given value. Indicates a range. If a range of values ​​is given, unless otherwise explicitly specified in the context, the lower bound is used. Each value between the upper and lower limits of the range, up to one-tenth of the unit, and within the specified range Other specified or existing values ​​are understood to be included in this disclosure. These small scope The upper and lower limits of the enclosure can be independently included within that smaller range, and also, specifically within the defined range Subject to any excluded limits, this disclosure includes the specified range, which is either one of the limits or If both are included, the range excluding one or both of these included limits is also the main limit. It is included in the diagram.

[0124] As used herein and in its embodiments, the indefinite articles "a" and "an" are not particularly Unless otherwise specified, it shall be understood that this means "at least one".

[0125] As used herein and in its embodiments, the expression "and / or" " is "one or both" of the elements that are thus joined, that is, in some cases, connective It is understood that this refers to elements that exist in one place and, in other cases, exist separately. Multiple elements listed by "and / or" are similar in that way. And, it is interpreted as "one or more" of the elements that are combined in that way. The elements specifically identified in the "and / or" clause are optional. Whether related or not, other elements other than the specifically identified elements exist. It is also acceptable to use "A and / or B" as a non-restrictive example. The reference "B)" is an open-ended expression such as "comprising". When used in combination with, in one embodiment, only A is represented (optionally, other elements other than B may be included). (including elements), in another embodiment, B only (optionally, elements other than A) In another embodiment, both A and B may be represented (optionally). (including other elements).

[0126] As used herein and in embodiments, “or” means “or” as defined above. It is understood that this has the same meaning as "and / or". For example, when separating items in a list, you can use "or" or "and / or (a "nd / or)" is inclusive, meaning at least one of many elements or elements of a list. Includes one item, and also includes two or more items, and optionally includes additional items not on the list. This is interpreted as "only one of" or "of the..." "exactly one of," or the place of use in the embodiment Terms that have a particularly clear designation, such as "consisting of" Therefore, it can only be expressed that it contains exactly one of many elements or elements from a list. As used herein, the term "or" generally means "either one." her), one of, only one of "one of" or "exactly one of" The term "exclusive choice" (i.e., "one or the other") is only used when preceded by a term indicating exclusivity, such as "one or the other." It is one or the other, but not both (bot) h) is interpreted as indicating "~essentially constitutes" The phrase "(consisting essentially of)" is used in the field of patent law. It shall have the usual meaning of "to be."

[0127] Refer to a list of one or more elements as used in this specification and its embodiments. The expression "at least one" means that there is at least one element in the list of elements. It is understood that this means at least one element selected from multiple arbitrary elements. Nono, at least one of every element specifically listed within the list of elements This does not include any combination of elements in the list. If so, as an optional choice, the expression "at least one" represents the essential Regardless of whether or not it relates to an element specifically identified within the raw list, specifically identified Other elements may exist besides the element that was selected. For this reason, as a non-restrictive example, "A and "at least one of A and B" (or equivalent) In terms of value, "at least one of A or B" B)" or equivalently "at least one of A and / or B (at least o "(one of A and / or B))" is, in one embodiment, a case where B does not exist. As an optional choice, include at least one A that contains two or more (optionally, include elements other than B) It can represent (including). In another embodiment, A does not exist, and optionally, two or more This includes the above, and represents at least one B (optionally including elements other than A), and further In another embodiment, optionally, at least one A and including two or more Optionally, include at least one B (and optionally, other It can represent elements, etc.

[0128] In addition to the embodiments, the above specification includes "comprising" and "including". "including", "carrying", "having" ng), containing, involving, preserving All words such as "holding" and "composed of" The transition clause is open-ended, that is, it includes non-restrictively (include It is understood that this means "ng but not limited to". The transitional phrases "consisting of" and "essentially consisting of" Only the patent examiner at the United States Patent and Trademark Office stated that "sisting essentially of" As stipulated in Section 2111.03 of the Continued Manual, each is either closed or semi-closed. This is a transitional clause.

[0129] While specific embodiments of this disclosure have been outlined above, many alternatives, improvements, and modifications will become apparent to those skilled in the art. Therefore, the embodiments described herein are illustrative and not intended to be limiting in any way. Various modifications are possible without departing from the spirit and scope of this disclosure. Where the above-described methods and steps indicate specific events occurring in a specific order, those skilled in the art who benefit from this disclosure will recognize that the order of the specific steps can be changed, and such changes constitute modifications of the invention. Furthermore, in addition to performing specific steps sequentially as described above, parallel processes can be performed simultaneously where possible. While embodiments have been illustrated and described in detail above, it will be understood that various modifications are possible in form and detail. This specification discloses the inventions described below. [Configuration 1] A kit for preparing a cell suspension, An apparatus for defining a first container and a second container, comprising a first label for identifying the first container used in a first part of a tissue processing method, and a second label for identifying the second container used in a second part of the tissue processing method, A housing configured to receive the aforementioned device, A first housing portion configured to house a first set of components associated with the first part of the tissue processing method, and including a first visual indicator associated with the first label of the apparatus, A second housing portion configured to house a second set of components associated with the second part of the tissue processing method, and including a second visual indicator associated with the second label of the apparatus, Including housing, A kit that includes everything. [Configuration 2] The kit according to configuration 1, wherein the first housing portion is integrally formed with the second housing portion in an integrated tray portion. [Configuration 3] The kit according to configuration 2, wherein the integrated tray portion has a recess configured to receive the device. [Structure 4] The kit according to configuration 1, wherein the first housing portion is separated from the second housing portion. [Composition 5] The kit according to configuration 1, wherein the first housing portion is connected to the second housing portion via a perforated connecting portion. [Composition 6] The kit according to configuration 1, wherein the housing includes a base configured to receive the first housing portion and the second housing portion. [Composition 7] The kit according to Configuration 1, wherein the apparatus includes a third label defining a third container and identifying the third container for use in a third part of a tissue processing method, and the housing includes a third housing section configured to house a third set of components associated with the third part of the tissue processing method, and includes a third visual indicator associated with the third label of the apparatus. [Structure 8] The kit according to configuration 7, wherein the housing includes a base configured to receive the first housing portion, the second housing portion, and the third housing portion. [Composition 9] The kit according to configuration 1, wherein the first set of components comprises an enzyme vial, and the first housing portion includes a recess configured to receive the enzyme vial. [Configuration 10] The system according to configuration 1, wherein the first visual indicator and the first label each include a first color, the second visual indicator and the second label each include a second color, and the third visual indicator and the third label each include a third color. [Composition 11] The system according to configuration 1, wherein the first visual indicator and the first label each include a first character, the second visual indicator and the second label each include a second character, and the third visual indicator and the third label each include a third character. [Composition 12] The system according to configuration 1, wherein the second part of the tissue processing method is executed sequentially after the first part of the tissue processing method. [Composition 13] The system according to configuration 1, wherein the apparatus is enclosed by side walls and includes a skin tissue manipulation area that includes a textured surface area. [Composition 14] The system according to configuration 1, wherein the first set of components includes a vial containing an enzyme. [Composition 15] The system according to configuration 1, wherein the second set of components includes a vial containing a buffer. [Composition 16] A packaging system for an apparatus for preparing cell suspensions, wherein the apparatus comprises a first container having a first label and a second container having a second label, A first housing portion comprising a set of recesses configured to receive a first set of components associated with a first part of a tissue processing method, and including a first visual indicator associated with the first label of the apparatus, A second housing portion comprising a set of recesses configured to receive a second set of components associated with the second part of the tissue processing method, and including a second visual indicator associated with the second label of the apparatus, A packaging system equipped with [specific features / features]. [Composition 17] The packaging system according to configuration 16, wherein the first housing portion is integrally formed with the second housing portion in an integrated tray portion. [Composition 18] The packaging system according to configuration 17, wherein the integrated tray portion has a recess configured to receive the device. [Composition 19] The packaging system according to configuration 16, wherein the first housing portion is separated from the second housing portion. [Configuration 20] The packaging system according to configuration 16, wherein the first housing portion is connected to the second housing portion via a perforated connecting portion. [Composition 21] The packaging system according to configuration 16, further comprising a base configured to receive the first housing portion and the second housing portion. [Composition 22] The packaging system according to configuration 16, wherein the apparatus includes a third label that defines a third container and identifies the third container for use in a third part of a tissue processing method, and the packaging system further includes a third housing portion configured to house a third set of components associated with the third part of the tissue processing method, and includes a third visual indicator associated with the third label of the apparatus. [Composition 23] The packaging system according to configuration 22, further comprising a base configured to receive the first housing portion, the second housing portion, and the third housing portion. [Composition 24] A method for preparing a cell suspension using a device packaged in a housing, Identifying a first matching between a first label disposed in close proximity to the first container of the device and a first visual indicator contained in the first housing portion of the housing, In response to the identification of the first matching, a first set of components is removed from the first housing, and a first part of the tissue processing method associated with the first container is performed using the first set of components. Identifying a second matching between a second label disposed in close proximity to the second container of the device and a second visual indicator included in the second housing portion of the housing, In response to the identification of the second matching, a second set of components is removed from the second housing, and the second part of the tissue processing method associated with the second container is performed using the second set of components. Methods that include... [Composition 25] The method according to configuration 24, further comprising moving the first housing portion to a sterilization area in response to the identification of the first matching. [Composition 26] The method according to configuration 24, further comprising moving the second housing portion to a sterilization area in response to the identification of the second matching. [Composition 27] Identifying a third matching between a third label disposed in close proximity to the third container of the device and a third visual indicator included in the third housing portion of the housing, In response to the identification of the third matching, a third set of components is removed from the third housing, and the third part of the tissue processing method associated with the third container is performed using the third set of components. Methods of configuration 24, further including [Composition 28] The method according to configuration 24, further comprising performing the tissue manipulation unit of the tissue processing method on the preparation tray of the apparatus. [Composition 29] The first set of components comprises a vial of sterile water, a syringe, and an enzyme vial, and the first part of the tissue processing method associated with the first container is To prepare an enzyme mixture, The enzyme mixture is delivered to the first container, A method of configuration 24, including the method described in configuration 24. [Composition 30] Removing the enzyme vial from the sterile pouch, The enzyme vial is placed in the recess defined in the first housing portion, The method described in configuration 24, further including the method described in configuration 24. [Composition 31] The second set of components includes a buffer vial and a syringe, and the second part of the tissue processing method associated with the second container is Drawing a certain volume of the buffer from the buffer vial into the syringe, The aforementioned volume of the buffer is delivered from the syringe to the second container, A method of configuration 24, including the method described in configuration 24. [Composition 32] The third set of components includes a syringe, and the third part of the tissue processing method associated with the third container is Disassembling skin samples, To generate a cell suspension containing cells from the aforementioned decomposed skin sample, Drawing the cell suspension into the syringe, A method of configuration 25, including the method described in configuration 25.

Claims

1. A kit for preparing a cell suspension, An apparatus defining a first container used in a first part of a tissue processing method for preparing the cell suspension, a second container used in a second part of the tissue processing method, and a third container used in a third part of the tissue processing method, A preparation tray used for performing at least the third part of the tissue processing method, Equipped with, The first part includes preparing an enzyme mixture and delivering the enzyme mixture to a first container. The second part includes preparing a buffer and delivering the buffer to the second container. The preparation tray includes one or more collection areas disposed on the bottom surface of the preparation tray, the bottom surface includes a skin tissue manipulation area where the cell suspension is prepared, and the bottom surface is configured such that the cell suspension flows along a transition surface having a slope from the skin tissue manipulation area toward the one or more collection areas. The third portion includes removing the skin sample immersed in the enzyme mixture in the first container from the first container, disintegrating the skin sample in the skin tissue manipulation area of ​​the preparation tray, generating the cell suspension containing cells from the disintegrated skin sample, and drawing the cell suspension from one or more collection areas into a syringe. The bottom surface is surrounded by side walls, The skin tissue manipulation area includes a textured surface area. kit.

2. The kit according to claim 1, wherein the preparation tray is an insert tray that can be removed from the complementary concave area of ​​the apparatus.

3. The kit according to claim 1, wherein the bottom surface of the preparation tray includes a main surface and a lower surface, the transition surface is inclined from the main surface to the lower surface, and the main surface constitutes the skin tissue manipulation area.

4. The third container contains a cell filter, The kit according to claim 1, wherein the cell suspension is dispensed from the syringe through the cell filter into the third container.

5. A method for preparing the cell suspension using the kit described in any one of claims 1 to 3, Performing the first part of the tissue processing method associated with the first container, Performing the second portion of the tissue processing method associated with the second container, Performing the third portion of the tissue processing method associated with the third container, Methods that include...

6. The method according to claim 5, wherein a cell filter is placed in the third container.

7. The method according to claim 6, wherein the cell suspension is dispensed from the syringe through the cell filter into the third container.

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