Functional composition for relieving hangovers containing seahorse extract as an effective ingredient

Abstract

The present invention relates to a functional composition for relieving hangovers comprising seahorse extract as an active ingredient. By consuming the composition of the present invention, symptoms such as headache, nausea, and fatigue caused by a hangover are alleviated, and liver function is improved, thereby increasing the speed of recovery after drinking. Furthermore, the use of seahorse extract, a natural ingredient, has the effect of enhancing safety.

Description

Technology Field

[0001] The present invention relates to a functional composition for relieving hangovers comprising a seahorse extract as an active ingredient. The functional composition of the present invention may be used as a health functional food composition or a pharmaceutical composition for relieving hangovers. Background Technology

[0003] A hangover refers to symptoms such as headache, nausea, fatigue, and difficulty concentrating that occur after excessive drinking, causing significant inconvenience in daily life. Hangovers are primarily caused by the accumulation of a toxic substance called acetaldehyde, which is produced during the metabolism of alcohol. Acetaldehyde remains in the body until it is converted into acetic acid by alcohol dehydrogenase in the liver, triggering hangover symptoms.

[0004] Currently, various products designed to alleviate hangovers are available on the market. These products primarily aim to relieve symptoms by including ingredients that promote the metabolism of acetaldehyde or support liver function. However, these products have shown minimal effectiveness in some users, and side effects may occur with long-term use. Consequently, there is a growing demand for safer and more effective hangover relief formulations utilizing natural ingredients.

[0005] Seahorses have traditionally been known for their various health-promoting effects; in particular, Eastern medicine records their efficacy in strengthening liver function, relieving fatigue, and boosting metabolism. Seahorse extracts can help protect the liver through antioxidant action and enhance detoxification functions. These properties are considered to be highly useful for relieving hangovers.

[0006] In particular, seahorses contain various bioactive substances that aid the body's metabolic activity, making them effective for the rapid elimination of toxins caused by hangovers and the improvement of liver function. Compared to existing hangover remedies, compositions using seahorse extract have the advantage of being based on natural ingredients, which reduces concerns about side effects and ensures high safety even with long-term consumption.

[0007] Against this backdrop, the present invention aims to provide a functional composition effective for relieving hangovers by including seahorse extract as a major active ingredient. Prior art literature

[0009] 10-1928883 The problem to be solved

[0010] One objective of the present invention to solve the above problems is to provide a health functional food composition for hangover relief comprising a seahorse extract as an active ingredient.

[0011] In addition, one objective of the present invention is to provide a pharmaceutical composition for relieving hangovers comprising a seahorse extract as an active ingredient. means of solving the problem

[0013] To achieve the above objectives, the present invention provides a food composition for relieving hangovers comprising a seahorse extract as an active ingredient.

[0014] In one embodiment of the present invention, the "seahorse extract" may be extracted using one or more organic solvents selected from the group consisting of alcohols having 1 to 4 carbon atoms, such as methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane, and cyclohexane, and preferably extracted using 30 to 70% ethanol as a solvent, but is not limited thereto.

[0015] In one embodiment of the present invention, the "seahorse extract" may be included at a concentration of 10 to 100 mg / ml, preferably at a concentration of 30 to 70 mg / ml, and more preferably at a concentration of 40 to 60 mg / ml, but is not limited thereto.

[0016] In addition, to achieve the above objectives, the present invention provides a health functional food composition for relieving hangovers comprising a seahorse extract as an active ingredient.

[0017] Finally, to achieve the above objectives, the present invention provides a pharmaceutical composition for relieving hangovers comprising a seahorse extract as an active ingredient. Effects of the invention

[0019] The present invention relates to a functional composition for relieving hangovers comprising seahorse extract as an active ingredient. By consuming the composition of the present invention, symptoms such as headache, nausea, and fatigue caused by a hangover are alleviated, and liver function is improved, thereby increasing the speed of recovery after drinking. Furthermore, the use of seahorse extract, a natural ingredient, has the effect of enhancing safety. Brief explanation of the drawing

[0021] Figure 1 is a photograph of seahorse powder obtained by crushing the seahorse used in the present invention in one embodiment of the present invention, and is a figure showing the result of analyzing the general components thereof (**requested from an analysis center). FIG. 2 is a diagram showing the manufacturing process of the seahorse extract of the present invention in one embodiment of the present invention. Figure 3 is a figure showing the ADH activity-promoting effect of the hippocampus extract of the present invention in one embodiment of the present invention. Figure 4 is a figure showing the ALDH activity-promoting effect of the seahorse extract of the present invention in one embodiment of the present invention. Specific details for implementing the invention

[0022] Hereinafter, the present invention will be described in detail with reference to the attached drawings and embodiments thereof. However, the following embodiments are presented as examples of the present invention, and if it is determined that a detailed description of a technology or configuration well known to those skilled in the art may unnecessarily obscure the essence of the present invention, such detailed description may be omitted, and the present invention is not limited thereby. The present invention is capable of various modifications and applications within the scope of the claims set forth below and the equivalent scope interpreted therefrom.

[0023] Furthermore, the terminology used in this specification is used to appropriately describe preferred embodiments of the present invention, and may vary depending on the intent of the user or operator, or the conventions of the field to which the present invention belongs. Accordingly, the definitions of these terms should be based on the content throughout this specification. Throughout the specification, when a part is described as "comprising" a certain component, unless specifically stated otherwise, this means that it may include additional components rather than excluding other components.

[0024] Throughout this specification, '%' used to indicate the concentration of a particular substance is (w / w) % for solid / solid, (w / v) % for solid / liquid, and (v / v) % for liquid / liquid, unless otherwise noted.

[0026] In one aspect, the present invention provides a functional composition for relieving hangovers comprising a seahorse extract as an active ingredient.

[0027] The extract according to the present invention may be obtained by extracting and separating from "seahorses" using extraction and separation methods known in the art, and the "extract" defined in the present invention is extracted from "seahorses" using a suitable solvent, and includes, for example, crude extracts, extracts soluble in polar solvents, or extracts soluble in non-polar solvents. As a suitable solvent for preparing the above extract, any organic solvent permitted in food science or pharmaceuticals may be used, and water or organic solvents may be used, but are not limited thereto, various solvents such as purified water, alcohols having 1 to 4 carbon atoms including methanol, ethanol, propanol, isopropanol, butanol, acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane, and cyclohexane may be used alone or in combination. As an extraction method, any one of the following methods may be selected and used: hot water extraction, cold maceration extraction, reflux extraction, solvent extraction, steam distillation, ultrasonic extraction, elution, or pressing. In addition, the desired extract may further undergo a conventional fractionation process and may be purified using a conventional purification method.

[0028] There are no limitations on the method for preparing the extract of the present invention, and any known method may be used. For example, the extract included in the composition of the present invention may be prepared in a powder state by additional processes such as spray drying, vacuum distillation, or freeze-drying, using a primary extract obtained by the aforementioned hot water extraction or solvent extraction method. Additionally, a further purified fraction may be obtained from the primary extract using various chromatographic methods such as silica gel column chromatography, thin layer chromatography, or high performance liquid chromatography. Accordingly, in the present invention, the term "extract" encompasses all extracts, fractions, and purified products obtained at each stage of extraction, fractionation, or purification, as well as their dilutions, concentrates, or dried products.

[0030] In one aspect, the present invention provides a food or health functional food composition for relieving hangovers comprising a seahorse extract as an active ingredient.

[0031] In addition to containing an extract as an active ingredient, the food or health functional food composition of the present invention may contain various flavoring agents or natural carbohydrates, etc., as additional ingredients, as in conventional food or health functional food compositions.

[0032] Examples of the natural carbohydrates described above include monosaccharides, e.g., glucose, fructose, etc.; disaccharides, e.g., maltose, sucrose, etc.; polysaccharides, e.g., dextrin, cyclodextrin, etc., and conventional sugars, and sugar alcohols such as xylitol, sorbitol, erythritol, etc. The flavoring agents described above may advantageously use natural flavoring agents (taumatin), stevia extracts (e.g., rebaudioside A, glycyrrhizin, etc.), and synthetic flavoring agents (saccharin, aspartame, etc.). The food composition of the present invention may be formulated in the same manner as the pharmaceutical composition described above and used as a functional food or added to various foods. Foods to which the composition of the present invention may be added include, for example, beverages, meat, chocolate, food products, confectionery, pizza, ramen, other noodles, chewing gum, candy, ice cream, alcoholic beverages, vitamin complexes, and health supplements.

[0033] In addition, the above food or health functional food composition may contain, in addition to the extract which is an active ingredient, various nutritional supplements, vitamins, minerals (electrolytes), flavoring agents such as synthetic and natural flavoring agents, coloring agents and fillers (cheese, chocolate, etc.), pectic acid and its salts, alginic acid and its salts, organic acids, protective colloidal thickeners, pH adjusters, stabilizers, preservatives, glycerin, alcohol, carbonating agents used in carbonated beverages, etc. Furthermore, the food composition of the present invention may contain fruit pulp for the production of natural fruit juice, fruit juice beverages, and vegetable beverages.

[0034] The health functional food composition of the present invention may be manufactured and processed in the form of tablets, capsules, powders, granules, liquids, pills, etc. In the present invention, the term "health functional food composition" refers to a food manufactured and processed using raw materials or ingredients having functional properties useful to the human body pursuant to Article 6727 of the Health Functional Foods Act, and means consuming it for the purpose of obtaining effects useful for health uses, such as regulating nutrients or physiological actions regarding the structure and function of the human body. The health functional food of the present invention may include conventional food additives, and unless otherwise stipulated, suitability as a food additive is determined according to the specifications and standards for the relevant item in accordance with the general provisions and general test methods of the Food Additives Codex approved by the Ministry of Food and Drug Safety. Items listed in the aforementioned "Food Additives Codex" include, for example, chemically synthesized compounds such as ketones, glycine, calcium citrate, nicotinic acid, and cinnamon acid; and natural additives such as persimmon dye, licorice extract, crystalline cellulose, sorghum dye, and guar gum. Examples include mixed preparations such as L-sodium glutamate preparations, alkaline additives for noodles, preservative preparations, and tar dye preparations. For instance, a health functional food in tablet form may be produced by granulating a mixture of the active ingredient of the present invention with excipients, binders, disintegrants, and other additives using a conventional method, and then compression molding by adding a lubricant, etc., or by directly compression molding the mixture. Additionally, the health functional food in tablet form may contain a binder, etc., as necessary. Among health functional foods in capsule form, hard capsules may be manufactured by filling a conventional hard capsule with a mixture of the active ingredient of the present invention mixed with additives such as excipients, and soft capsules may be manufactured by filling a capsule base such as gelatin with a mixture of the active ingredient of the present invention mixed with additives such as excipients. The soft capsules may contain plasticizers such as glycerin or sorbitol, coloring agents, preservatives, etc., as necessary.A health functional food in the form of a pill can be prepared by molding a mixture of the active ingredient of the present invention, excipients, binders, disintegrants, etc., using a previously known method, and if necessary, it can be coated with sucrose or other coating agents, or the surface can be coated with a substance such as starch or talc. A health functional food in the form of a granule can be prepared by making a mixture of the active ingredient of the present invention, excipients, binders, disintegrants, etc., into a granular form using a previously known method, and may contain flavoring agents, stimulating agents, etc., if necessary.

[0036] In one aspect, the present invention provides a pharmaceutical composition for relieving hangovers comprising a seahorse extract as an active ingredient.

[0037] The pharmaceutical composition of the present invention may additionally include an adjuvant in addition to the active ingredient. Any adjuvant known in the art may be used without limitation, but, for example, Freund's complete or incomplete adjuvant may be further included to increase its functionality.

[0038] The pharmaceutical composition according to the present invention may be prepared in a form in which an active ingredient is incorporated into a pharmaceutically acceptable carrier. Here, the pharmaceutically acceptable carrier includes carriers, excipients, and diluents commonly used in the pharmaceutical field. Pharmaceutically acceptable carriers that can be used in the pharmaceutical composition of the present invention are not limited to these, but may include lactose, dextrose, sucrose, sorbitol, mannitol, xylitol, erythritol, maltitol, starch, acacia gum, alginate, gelatin, calcium phosphate, calcium silicate, cellulose, methylcellulose, polyvinylpyrrolidone, water, methylhydroxybenzoate, propylhydroxybenzoate, talc, magnesium stearate, and mineral oil.

[0039] The pharmaceutical composition of the present invention may be formulated and used in the form of oral formulations such as powders, granules, tablets, capsules, suspensions, emulsions, syrups, and aerosols, external formulations, suppositories, or sterile injectable solutions, each according to conventional methods.

[0040] When formulating, the product may be prepared using diluents or excipients such as commonly used fillers, volume expanders, binders, wetting agents, disintegrants, and surfactants. Solid dosage forms for oral administration include tablets, pills, powders, granules, and capsules, and such solid dosage forms may be prepared by mixing at least one excipient, such as starch, calcium carbonate, sucrose, lactose, gelatin, etc., with the active ingredient. In addition, lubricants such as magnesium stearate and talc may also be used in addition to simple excipients. Liquid dosage forms for oral administration include suspensions, liquid formulations, emulsions, and syrups, and may contain various excipients, such as wetting agents, sweeteners, flavoring agents, and preservatives, in addition to commonly used diluents such as water and liquid paraffin. Preparations for parenteral administration include sterile aqueous solutions, water-insoluble solvents, suspensions, emulsions, lyophilized preparations, and suppositories. Water-insoluble solvents and suspensions may include propylene glycol, polyethylene glycol, vegetable oils such as olive oil, and injectable esters such as ethyl oleate. Bases for suppositories may include Witepsol, Tween 61, cocoa paste, laurin paste, glycerogelatin, etc.

[0041] The pharmaceutical composition according to the present invention may be administered to an individual by various routes. Any mode of administration may be anticipated, for example, by oral, intravenous, intramuscular, subcutaneous, or intraperitoneal injection.

[0042] The dosage of the pharmaceutical composition according to the present invention is selected by taking into consideration the age, weight, gender, physical condition, etc. of the individual. It is obvious that the concentration of the active ingredient included in the pharmaceutical composition can be selected in various ways depending on the subject, and preferably, it is included in the pharmaceutical composition at a concentration of 0.01 to 5,000 μg / ml. If the concentration is less than 0.01 μg / ml, pharmaceutical activity may not appear, and if it exceeds 5,000 μg / ml, it may exhibit toxicity to the human body.

[0043] The above pharmaceutical composition can be formulated into various oral or parenteral administration forms.

[0044] Oral dosage forms include, for example, tablets, pills, hard and soft capsules, liquids, suspensions, emulsifiers, syrups, granules, etc., and these dosage forms may additionally contain diluents (e.g., lactose, dextrose, sucrose, mannitol, sorbitol, cellulose and / or glycine) and lubricants (e.g., silica, talc, stearic acid and its magnesium or calcium salts and / or polyethylene glycol) in addition to the active ingredient. Furthermore, the tablet may contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose and / or polyvinylpyrrolidine, and in some cases may contain disintegrants or boiling mixtures such as starch, agar, alginic acid or its sodium salt and / or absorbents, coloring agents, flavoring agents and sweeteners. The above formulation can be prepared by conventional mixing, granulation, or coating methods.

[0045] In addition, representative formulations for parenteral administration are injectable preparations, and solvents for injectable preparations include water, Ringer's solution, isotonic saline, or suspensions. The sterile fixation oil of the above injectable preparation can be used as a solvent or suspension medium, and any non-irritating fixation oil, including mono- and di-glycerides, can be used for this purpose.

[0046] In addition, the above-mentioned injectable formulation may use fatty acids such as oleic acid.

[0048] The present invention will be explained in more detail below through examples. However, the above examples and experimental examples are presented as illustrative examples of the present invention, and if it is determined that a detailed description of a technology or configuration well known to those skilled in the art may unnecessarily obscure the essence of the present invention, such detailed description may be omitted, and the present invention is not limited by this. The present invention is capable of various modifications and applications within the scope of the claims set forth below and the equivalent scope interpreted therefrom.

[0050] <Preparation Example> Preparation of seahorse extract

[0051] 300g of seahorse was ground using a grinder (Fig. 1). The moisture, ash, crude fat, and crude protein content of the ground seahorse powder were submitted to **Analysis Center Co., Ltd. for analysis. The measurements were taken based on the Food Code Section 8. General Test Methods / 2. Test Methods for Food Components / 2.1 General Component Test Methods. The results are shown in Fig. 1. The moisture content was confirmed to be 6.5%, the ash content 19.5%, the crude fat content 5.6%, and the crude protein content 60.8%.

[0052] The above seahorse powder was mixed with 20 times the amount of 50% EtOH solution, and extraction was performed at 70°C for 4 hours while stirring. The extract was filtered through a 200-mesh sieve and then concentrated under reduced pressure. The concentrate was freeze-dried, and the experiment was conducted using the freeze-dried powder (Fig. 2).

[0054] <Experimental Example 1> Analysis of Hangover Relief Efficacy of Seahorse Extract - Measurement of ADH Activity Promotion Effect

[0055] ADH activity was measured by slightly modifying the methods of Yoo et al. (2009) and Racker (1995), measuring the amount of NADH produced at an absorbance of 340 nm, and comparing it as relative activity to a control group. For the ADH enzymatic reaction, 1 ml of 0.01 M sodium phosphate (pH 7.0), 0.3 ml of 50 mM NAD (69840024, Racker), 1 ml of distilled water, 0.2 ml of the sample, and 0.2 ml of 3 mg / ml Alcohol dehydrogenase from Saccharomyces cerevisiae (A7011, SIGMA-ALDRICH) were mixed. After preincubation at 30°C for 5 minutes, 0.3 ml of ethanol was added and reacted at 30°C for 5 minutes, after which the absorbance was measured at 340 nm. The results showed the relative activity (%) of the experimental group, with the intrinsic ADH activity without added sample set to 100% as the control (Table 1 and Figure 3).

[0057] [Mathematical Formula 1]

[0058] ADH activity (%) = (B / A) Х 100

[0059] A: Absorbance of the control group, B: Absorbance of the experimental group

[0061]

[0063] As a result of measuring the ADH activity of seahorse extract, it was found that at concentrations of 25 and 50 mg / ml, the seahorse extract showed an ADH activity of 206.1% and 204.2%, respectively, which is more than twofold higher than the control. The ADH activity of Hovenia dulcis extract was measured as a comparative control, and at concentrations of 25 and 50 mg / ml, it showed an increase in ADH activity compared to the control, at 108.7% and 126.4%. The seahorse extract demonstrated a higher ADH activity-promoting effect than the comparative control, Hovenia dulcis extract.

[0065] <Experimental Example 2> Analysis of Hangover Relief Efficacy of Seahorse Extract - Measurement of ALDH Activity Promotion Effect

[0066] ALDH activity was measured by slightly modifying the methods of Tottmar et al. (1973) and Yoo et al. (2009), utilizing the principle of generating NADH from NAD using an enzyme that produces acetate from acetaldehyde. For the ALDH enzymatic reaction, 1 ml of 0.1 M Tris HCl (pH 8.0), 0.3 ml of 50 mM NAD, 0.1 ml of 3 M KCl, 0.1 ml of 0.33 M 2-mercaptoethanol, 0.8 ml of distilled water, 0.2 ml of the sample, and 0.2 ml of aldehyde dehydrogenase, potassium-activated from baker's yeast (S. cerevisiae, A6338, SIGMA-ALDRICH) were mixed and preincubated at 30°C for 5 minutes. After preincubation, 0.3 ml of 0.1 M acetaldehyde (402788, SIGMA-ALDRICH) was added and reacted at 30°C for 5 minutes, and the absorbance was measured at 340 nm. The results represent the relative activity (%) of the experimental groups, with the intrinsic activity of ALDH without added sample set as 100% as the control (Table 2 and Fig. 4).

[0068] [Mathematical Formula 2]

[0069] ALDH activity (%)= (B / A) Х 100

[0070] A: Absorbance of the control group, B: Absorbance of the experimental group

[0072]

[0074] As a result of measuring the ALDH activity of seahorse extract, it showed 187.0% ALDH activity at a concentration of 50 mg / ml, which is higher than the control, indicating an ALDH activity increase of nearly 1.87 times. The ALDH activity of Hovenia dulcis extract was measured as a comparative control, and at a concentration of 50 mg / ml, it showed 107.8%, an increase compared to the control. Compared to the comparative control Hovenia dulcis extract, seahorse extract demonstrated a high ALDH activity-promoting effect.

[0076] As described above, specific embodiments of the present invention have been described in detail; however, those skilled in the art who understand the spirit of the present invention will be able to easily propose other inventions that are inferior or other embodiments included within the scope of the spirit of the present invention by adding, changing, or deleting other components within the same spirit. Therefore, the embodiments described above should be understood as illustrative in all respects and not restrictive. The scope of the present invention is defined by the claims set forth below rather than by the detailed description above, and all modifications or variations derived from the meaning and scope of the claims and equivalent concepts should be interpreted as being included within the scope of the present invention.

Claims

Claim 1 A food composition for relieving hangovers containing seahorse extract as an active ingredient. Claim 2 A food composition for relieving hangovers according to claim 1, wherein the seahorse extract is extracted using one or more organic solvents selected from the group consisting of alcohols having 1 to 4 carbon atoms, such as methanol, ethanol, propanol, isopropanol, butanol, etc., acetone, ether, benzene, chloroform, ethyl acetate, methylene chloride, hexane, and cyclohexane. Claim 3 A food composition for relieving hangovers according to claim 1, characterized in that the seahorse extract is extracted using 30-70% ethanol as a solvent. Claim 4 A health functional food composition for hangover relief containing seahorse extract as an active ingredient. Claim 5 A pharmaceutical composition for relieving hangovers containing seahorse extract as an active ingredient.

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