Method for isolating pyocyanin from bacterial culture medium using the process of adsorption and desorption on aluminosilicate

Abstract

The subject of the application is a method for isolating pyocyanin from a microbiological medium using the process of adsorption and desorption on aluminosilicate, which is characterized in that pyocyanin is extracted from the microbiological medium with a hydrophobic ionic liquid, and then pyocyanin is isolated from the obtained system by adsorption on aluminosilicate, at a pH of the system equal to 3 or 7, at a temperature of 18°C ​​to 25°C, for 12 to 72 hours, with shaking, at a weight-to-volume ratio of aluminosilicate to hydrophobic ionic liquid of 1:4, and then the liquid phase is separated from the solid phase, which is then subjected to desorption with a solvent at a temperature of 18°C ​​to 25°C, for 12 to 72 hours, with shaking, at a weight-to-volume ratio of aluminosilicate to solvent of 1:4. 1:4.