Traditional chinese medicine composition for treating bronchial embolisms in poultry, preparation thereof, preparation method therefor and use thereof

Abstract

A traditional Chinese medicine composition for treating bronchial embolisms in poultry, a preparation thereof, a preparation method therefor and the use thereof. The traditional Chinese medicine composition comprises the following raw material components in parts by mass: 10-30 parts of Fagopyri dibotryis rhizoma, 5-15 parts of Lonicerae japonicae flos, 5-15 parts of Agrimoniae herba, 4-12 parts of Armeniacae semen amarum, 4-12 parts of Trichosanthis fructus, 2-6 parts of Borneolum syntheticum and 1-5 parts of Glycyrrhizae radix et rhizoma. Taking Fagopyri dibotryis rhizoma, Lonicerae japonicae flos and Agrimoniae herba as sovereign drugs, Armeniacae semen amarum and Trichosanthis fructus as minister drugs, Borneolum syntheticum as an assistant drug, and Glycyrrhizae radix et rhizoma as an envoy drug, the traditional Chinese medicine preparation for treating bronchial embolisms in poultry is prepared by means of a compatible combination of various drugs, which has a refined prescription, significant efficacy and an appropriate cost, and has good therapeutic effects on bronchial embolisms in poultry caused by the external contraction of wind-heat, pestilence, fever transformed from wind-cold invading the interior, etc. The problem that the clinical application effect of traditional Chinese medicine veterinary preparations for treating bronchial embolisms in poultry is poor in the prior art is effectively solved.

Description

A traditional Chinese medicine composition for treating poultry bronchial embolism, its preparation, preparation method and application

[0001] This patent application claims priority to Chinese patent application No. CN202311060692.4 filed on August 22, 2023. The disclosure of the prior application is incorporated herein by reference in its entirety. Technical Field

[0002] The present application relates to the field of traditional Chinese veterinary medicine, and specifically discloses a traditional Chinese medicine composition for treating poultry bronchial embolism, its preparation method and application. Background Art

[0003] Poultry farming environments are typically enclosed, especially during winter and spring. Ventilation is often avoided to conserve heat, leading to polluted air that can easily lead to respiratory infections. Autopsies of dead birds revealed large amounts of sputum in the trachea and yellowish-white, cheesy material blocking the bronchi. Bronchial embolism in poultry carries a high incidence, mortality, and long course, posing a significant threat to the poultry industry.

[0004] The main clinical symptoms and pathological changes of bronchial embolism in poultry include decreased feed intake, lethargy, difficulty breathing, panting with the neck stretched out, and unusual vocalizations. Most dead birds lie belly-up with their legs curled up. Autopsies primarily reveal the presence of copious amounts of sputum in the respiratory tract, sticky phlegm or a yellowish-white cheesy substance in the trachea, blockage of the bronchi by a yellowish-white cheesy substance, and congestion, hemorrhage, and blackening of the lungs.

[0005] The main pathogenesis of poultry bronchial embolism is: the body is affected by external evil (epidemic, wind-heat), the heat evil attacks the lungs, or is affected by wind-cold, which turns into heat, the body has a high fever, and is comatose and drowsy; heat toxins accumulate in the lungs, the lungs become purulent and ulcerated, forming lung abscesses, coughing up purulent sputum and yellow sputum; heat burns body fluids, which are refined into phlegm, phlegm heat blocks the lungs, and the lung qi does not descend, causing the body to cough and wheeze, phlegm heat stagnates for a long time, sputum becomes sticky, phlegm nodules are formed, the trachea is blocked, and breathing difficulties; in addition, heat forces blood to flow abnormally, causing blood to flow out of the blood vessels, leading to bleeding in the lungs, trachea, etc., and blood and phlegm mix to form bloody sputum.

[0006] Currently, poultry bronchial embolism is commonly treated clinically with antibiotics such as macrolides and tetracyclines, supplemented with respiratory antipyretics. There are also some compounded traditional Chinese veterinary medicines, among which Ma Xing Shi Gan oral liquid and Shuang Huang Lian oral liquid are commonly used. Ma Xing Shi Gan oral liquid has relatively weak effects on cooling blood, stopping bleeding, resolving phlegm, and awakening the mind, making it ineffective in controlling dyspnea and mortality caused by bronchial bleeding and bronchial embolism. Shuang Huang Lian oral liquid focuses on clearing heat, but lacks effectiveness in relieving cough, relieving asthma, resolving phlegm, cooling blood, stopping bleeding, and awakening the mind, resulting in unsatisfactory clinical results. Therefore, developing a traditional Chinese medicine preparation that can effectively treat poultry bronchial embolism is of great practical significance for poultry farming. Technical issues

[0007] In response to the problem that the clinical application effect of traditional Chinese veterinary preparations for treating poultry bronchial embolism in the prior art is not ideal, the present application provides a traditional Chinese medicine composition for treating poultry bronchial embolism, its preparation, preparation method and application. Technical Solutions

[0008] The technical solution adopted in this application is:

[0009] The first aspect of the present application provides a traditional Chinese medicine composition for treating poultry bronchial embolism, comprising the following raw material components in parts by weight: 10-30 parts of golden buckwheat, 5-15 parts of honeysuckle, 5-15 parts of agrimony, 4-12 parts of bitter almonds, 4-12 parts of trichosanthes, 2-6 parts of borneol and 1-5 parts of licorice.

[0010] Compared to the prior art, the present application provides a traditional Chinese medicine composition for treating poultry bronchial embolism. This application screens traditional Chinese medicines and selects a combination of herbs that have the effects of clearing heat and promoting lung function, resolving phlegm and relieving cough, cooling blood and stopping bleeding, eliminating carbuncle and draining pus, and awakening the mind and invigorating the mind. Among them, golden buckwheat, honeysuckle, and agrimony are the main herbs, bitter almond and trichosanthes are the auxiliary herbs, borneol is the adjuvant, and liquorice is the guiding herb.

[0011] Golden buckwheat tastes slightly pungent and astringent, is cool in nature, and enters the lung meridian. It has the effects of clearing away heat and detoxifying, clearing the lungs and discharging pus, and promoting blood circulation and removing blood stasis; honeysuckle tastes sweet and is cold in nature, and enters the lung, heart, and stomach meridians. It has the effects of clearing heat and detoxifying, and dispersing wind-heat; herb agrimony tastes bitter and astringent, is neutral in nature, and enters the heart, lung, and liver meridians. It has the effects of astringing, stopping bleeding, stopping dysentery, and detoxifying; bitter almond tastes bitter and is slightly warm in nature, and enters the lung and large intestine meridians. It has the effects of descending qi, relieving cough and asthma, and moistening the intestines and promoting bowel movements; trichosanthes tastes sweet and slightly bitter, is cold in nature, and enters the lung, stomach, and large intestine meridians. It clears heat and eliminates phlegm, relieves chest tightness and disperses stagnation, and moisturizes dryness and lubricates the intestines; borneol has a light fragrance, tastes pungent and bitter, is cool in nature, and enters the heart, spleen, and lung meridians. It has the effects of invigorating the mind, clearing away heat and relieving pain; licorice tastes sweet and is neutral in nature, and enters the twelve meridians. It has the effects of tonifying the middle and replenishing qi, removing phlegm and relieving cough, harmonizing the middle and relieving urgency, detoxifying, and harmonizing the properties of medicines.

[0012] The drug combinations described in this application, when combined and applied to poultry for bronchial embolism, can alleviate the pathogenesis, relieve clinical symptoms, shorten the course of treatment, and reduce mortality. The composition for treating bronchial embolism in poultry provided in this application is refined and has the effects of clearing the lungs and purging heat, cooling the blood and stopping bleeding, relieving cough and asthma, resolving phlegm, eliminating carbuncles and draining pus, and awakening the mind and invigorating the mind. Compared with existing large compound drugs, it has significant efficacy and is cost-effective. It has a good therapeutic effect on bronchial embolism caused by exogenous wind-heat, epidemic diseases, and wind-cold invading the interior and transforming into heat in poultry.

[0013] Preferably, the traditional Chinese medicine composition comprises the following raw material components in parts by weight: 15-30 parts of golden buckwheat, 8-15 parts of honeysuckle, 8-12 parts of agrimony, 6-12 parts of bitter almonds, 6-10 parts of trichosanthes, 3-6 parts of borneol and 2-4 parts of liquorice.

[0014] Further preferably, the traditional Chinese medicine composition comprises the following raw material components in parts by mass: 20-30 parts of golden buckwheat, 10-15 parts of honeysuckle, 10-12 parts of agrimony, 8-10 parts of bitter almonds, 6-10 parts of trichosanthes, 3-6 parts of borneol and 3 parts of licorice.

[0015] Further preferably, the Chinese medicine composition comprises the following raw material components in parts by mass: 25 parts of golden buckwheat, 10 parts of honeysuckle, 10 parts of agrimony, 8 parts of bitter almonds, 8 parts of trichosanthes, 6 parts of borneol and 3 parts of liquorice.

[0016] The second aspect of the present application provides a traditional Chinese medicine preparation for treating poultry bronchial embolism, including the traditional Chinese medicine composition for treating poultry bronchial embolism.

[0017] Preferably, the dosage form is any one of oral liquid or granules.

[0018] The Chinese medicine preparation of the present application can be made into oral liquid or granules according to the characteristics of medication in large-scale farming, and can be used for mixing with feed or drinking water.

[0019] The third aspect of the present application provides a method for preparing the traditional Chinese medicine preparation for treating poultry bronchial embolism, comprising the following steps:

[0020] Step 1: Weigh the components except borneol according to the designed ratio of raw material components, add water and boil 2-3 times, filter, and concentrate under reduced pressure until the relative density of the liquid is 1.02-1.06, and cool to obtain a concentrated solution;

[0021] Step 2: dissolving borneol in alcohol to obtain a borneol alcohol solution; adding an emulsifier to the borneol alcohol solution and mixing well to obtain a borneol mixed solution;

[0022] Step 3: Evenly mix the concentrated solution and the borneol mixture, add a preservative, add water to make the drug-liquid ratio 0.9-1:1-1.1, and sterilize at 120° C.-122° C. to obtain the traditional Chinese medicine preparation for treating poultry bronchial embolism.

[0023] The medicinal liquid ratio in step three refers to the ratio of the total mass of the raw material components golden buckwheat, honeysuckle, agrimony, bitter almond, trichosanthes, borneol and liquorice to the mass of the medicinal liquid obtained after adding water in step three.

[0024] Preferably, in step 1, the amount of water added is 8-14 times the mass of the drug.

[0025] Preferably, in step 1, the specific operation of decocting is to boil and then keep it slightly boiling for 30 minutes to 90 minutes.

[0026] Preferably, in step 2, the mass ratio of borneol to alcohol is 1:10-30.

[0027] Preferably, in step 2, the alcohol is ethanol with a mass concentration of 90%-95%.

[0028] Preferably, in step 2, the emulsifier is Tween 80.

[0029] Preferably, in step 2, the mass ratio of the emulsifier to the borneol solution is 1-3:100.

[0030] Preferably, in step three, the preservative is one or both of sodium benzoate and ethylparaben.

[0031] Preferably, in step three, the amount of the preservative added is 0.5wt%-0.6wt% of the total amount of the concentrated solution and the borneol mixture.

[0032] Preferably, in step three, the sterilization time is 15 min-20 min.

[0033] The fourth aspect of the present application provides a method for preparing the traditional Chinese medicine preparation for treating poultry bronchial embolism, comprising the following steps:

[0034] S1. Weigh the components except borneol according to the designed ratio of raw material components, add water and boil 2-3 times, filter, concentrate under reduced pressure, take a sample, cool the sample to 60°C-65°C, and measure the relative density. When the relative density is 1.08-1.25, stop the vacuum concentration, cool, and obtain a concentrated extract;

[0035] S2. Weigh the excipients, add the concentrated extract, mix evenly, granulate, and dry to obtain granules;

[0036] S3. Dissolving borneol in alcohol, spraying the mixture on the surface of the particles, and drying the mixture to obtain the traditional Chinese medicine preparation for treating poultry bronchial embolism.

[0037] Preferably, in S2, the auxiliary materials are powdered sugar and dextrin in a mass ratio of 3-10:1.

[0038] Preferably, in S2, the amount of the excipient is 3-6 times that of the concentrated extract. Beneficial effects

[0039] To address the problem of unsatisfactory clinical efficacy of existing Chinese veterinary medicine preparations for treating poultry bronchial embolism, the present application provides a Chinese medicine composition and preparation for treating poultry bronchial embolism. This application uses golden buckwheat, honeysuckle, herb agrimony, bitter almond, Trichosanthes kirilowii, borneol, and licorice as raw materials, and combines and compatibilizes the various drugs according to their monarch-minister-assistant-adjuvant relationship, and then applies them to poultry bronchial embolism. The Chinese medicine composition for treating poultry bronchial embolism provided in this application has a refined formula and has the effects of clearing the lungs and purging heat, cooling the blood and stopping bleeding, relieving cough and asthma, resolving phlegm, eliminating carbuncles and draining pus, and awakening the mind and invigorating the mind. Compared with large compound medicines in the prior art, it has significant efficacy and reasonable cost, and has a good therapeutic effect on bronchial embolism caused by exogenous wind-heat, epidemics, and wind-cold entering the interior of the body and transforming into heat in poultry. Modes for Carrying Out the Invention

[0040] The following is a clear and complete description of the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of them. Based on the embodiments of this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0041] Example 1

[0042] This embodiment provides a Chinese medicine composition and preparation for treating poultry bronchial embolism, the specific contents of which are as follows:

[0043] A traditional Chinese medicine composition for treating poultry bronchial embolism comprises the following raw material components in parts by weight: 25 parts of golden buckwheat, 10 parts of honeysuckle, 10 parts of agrimony, 8 parts of bitter almonds, 8 parts of trichosanthes, 6 parts of borneol, and 3 parts of liquorice.

[0044] A method for preparing a Chinese medicine preparation comprising the above-mentioned Chinese medicine composition for treating poultry bronchial embolism comprises the following steps:

[0045] Step 1, according to the mass fractions of the above raw material components, weigh the components other than borneol, add 14 times the mass of the drug, continue to boil after boiling, and simmer for 60 minutes, then add 10 times the mass of the drug, continue to boil after boiling, and simmer for 30 minutes, filter with a 100-mesh filter cloth after decoction, and concentrate the filtrate under reduced pressure to a relative density of 1.05, and cool to room temperature to obtain a concentrated solution;

[0046] Step 2: dissolving borneol in 95% ethanol at a mass ratio of borneol to alcohol of 1:10 to obtain a borneol alcohol solution, adding Tween 80 at a concentration of 2% by mass of the borneol alcohol solution, and mixing well to obtain a borneol mixed solution;

[0047] Step 3: Evenly mix the concentrate and the borneol mixture, add 0.5 wt% of sodium benzoate and 0.05 wt% of ethylparaben based on the total amount of the concentrate and the borneol mixture, add purified water to a drug-liquid ratio of 1:1, and sterilize at 121° C. for 15 minutes to obtain a traditional Chinese medicine preparation (oral solution) for treating poultry bronchial embolism.

[0048] Example 2

[0049] This embodiment provides a Chinese medicine composition and preparation for treating poultry bronchial embolism, the specific contents of which are as follows:

[0050] A traditional Chinese medicine composition for treating poultry bronchial embolism comprises the following raw material components in parts by weight: 25 parts of golden buckwheat, 10 parts of honeysuckle, 10 parts of agrimony, 8 parts of bitter almonds, 8 parts of trichosanthes, 6 parts of borneol, and 3 parts of liquorice.

[0051] A method for preparing a Chinese medicine preparation comprising the above-mentioned Chinese medicine composition for treating poultry bronchial embolism comprises the following steps:

[0052] S1. Weigh the components other than borneol according to the mass fractions of the above raw material components, add 14 times the mass of the drug, continue to keep it slightly boiling after boiling, and simmer for 60 minutes. Then add 10 times the mass of the drug, continue to keep it slightly boiling after boiling, and simmer for 30 minutes. After simmering, filter with a 100-mesh filter cloth, concentrate the filtrate under reduced pressure, take a sample, cool the sample to 60°C to measure the relative density, stop concentrating under reduced pressure when the relative density is 1.15, cool, and obtain a concentrated extract;

[0053] S2. Weigh 3 times the amount of powdered sugar and 1 times the amount of dextrin of the concentrated extract, add the concentrated extract while stirring, and stir until it forms a mass that falls apart when lightly squeezed. Sieve through a 14-mesh sieve and dry to obtain granules.

[0054] S3. Dissolve borneol in 95% ethanol at a mass ratio of borneol to alcohol of 1:10, spray the mixture on the surface of the granules, and dry the mixture to obtain a traditional Chinese medicine preparation (granules) for treating poultry bronchial embolism.

[0055] Example 3

[0056] This embodiment provides a Chinese medicine composition and preparation for treating poultry bronchial embolism, the specific contents of which are as follows:

[0057] A traditional Chinese medicine composition for treating poultry bronchial embolism comprises the following raw material components in parts by weight: 10 parts of golden buckwheat, 10 parts of honeysuckle, 5 parts of agrimony, 8 parts of bitter almonds, 8 parts of trichosanthes, 6 parts of borneol, and 3 parts of liquorice.

[0058] A method for preparing a Chinese medicine preparation comprising the above-mentioned Chinese medicine composition for treating poultry bronchial embolism comprises the following steps:

[0059] Step 1, according to the mass fractions of the above raw material components, weigh the components other than borneol, add 12 times the mass of the drug, continue to boil after boiling, and simmer for 90 minutes, then add 10 times the mass of the drug, continue to boil after boiling, and simmer for 60 minutes. After decocting, filter with a 100-mesh filter cloth, and concentrate the filtrate under reduced pressure to a relative density of 1.04, and cool to room temperature to obtain a concentrated solution;

[0060] Step 2: dissolving borneol in 95% ethanol at a mass ratio of borneol to alcohol of 1:10 to obtain a borneol alcohol solution, adding Tween 80 at a concentration of 2% by mass of the borneol alcohol solution, and mixing well to obtain a borneol mixed solution;

[0061] Step 3: Evenly mix the concentrate and the borneol mixture, add 0.45 wt% of sodium benzoate and 0.06 wt% of ethylparaben based on the total amount of the concentrate and the borneol mixture, add purified water to a drug-liquid ratio of 1:1.05, and sterilize at 121° C. for 20 minutes to obtain a traditional Chinese medicine preparation (oral solution) for treating poultry bronchial embolism.

[0062] Comparative Example 1

[0063] This comparative example provides a traditional Chinese medicine composition and preparation for treating poultry bronchial embolism. The difference from Example 1 is that the golden buckwheat is replaced by an equal amount of honey asters, and the agrimony is replaced by an equal amount of Imperata rhizome. The other components and preparation methods remain unchanged and are not further described here.

[0064] Comparative Example 2

[0065] This comparative example provides a traditional Chinese medicine composition and preparation for treating poultry bronchial embolism. The difference from Example 1 is that golden buckwheat is replaced by an equal amount of houttuynia cordata, and bitter almonds are replaced by an equal amount of honey-roasted loquat leaves. The other components and preparation methods remain unchanged and are not further described here.

[0066] To further illustrate the technical effects of this application, this application conducted a poultry bronchial embolization treatment test, the specific contents of which are as follows:

[0067] 1. Small-group precision therapy trials

[0068] Experimental animals: 24-day-old AA white-feathered broiler chickens raised in a broiler farm in Dalian, Liaoning.

[0069] Symptoms: A small group of chickens will grunt, stretch their necks and open their mouths to breathe (abdominal breathing), make strange noises, and their feces will be yellow-brown and thin; their feed intake will decrease, and seemingly healthy chickens will suddenly scream and die in the supine position.

[0070] Pathological autopsy: The autopsy showed that the trachea of ​​the dead chickens was congested and hemorrhagic, with white sticky sputum in the trachea, and bloody sputum in severe cases. There was yellow-white cheesy embolism in the lower end of the trachea to the bronchi, the lungs were congested and black, some lungs were edematous, and some had yellow-white block-like objects. The chest and abdominal air sacs were turbid and thickened, with foamy fibrinous exudates, and in severe cases, yellow cheesy objects were attached to the chest and abdominal air sacs.

[0071] Experimental groups: 350 broiler chickens with obvious and similar symptoms of bronchial embolism were selected from the above-mentioned sick chicken flocks and divided into experimental group 1, experimental group 2, experimental group 3, experimental group 4, experimental group 5, experimental group 6 and control group, with 50 chickens in each group. Each group was fed the same feed, and the rest of the feeding and management were the same except for the different medications.

[0072] Experimental drugs: the traditional Chinese medicine preparation for treating poultry bronchial embolism provided in Example 1 of the present application, the traditional Chinese medicine preparation for treating poultry bronchial embolism provided in Example 2 of the present application, the traditional Chinese medicine preparation for treating poultry bronchial embolism provided in Comparative Example 1 of the present application, the traditional Chinese medicine preparation for treating poultry bronchial embolism provided in Comparative Example 2 of the present application, 10% doxycycline hydrochloride soluble powder, Ma Xing Shi Gan oral liquid and Shuang Huang Lian oral liquid. All the drugs used are produced by Hebei Zhengyu Pharmaceutical Co., Ltd.

[0073] Test treatment:

[0074] In one experiment, the Chinese medicine preparation for treating poultry bronchial embolism provided in Example 1 of the present application was dissolved in water at a ratio of 1 mL: 2 L (Chinese medicine preparation: water), and the mixture was given to sick chickens as drinking water for 5 consecutive days.

[0075] Experimental Group 2: The Chinese medicine preparation for treating poultry bronchial embolism provided in Example 2 of the present application was dissolved in water at a ratio of 1 g: 2 L (Chinese medicine preparation: water) and given to sick chickens as drinking water for 5 consecutive days;

[0076] Experimental group three: Dissolve 10% doxycycline hydrochloride soluble powder in water at a ratio of 1g:1L (10% doxycycline hydrochloride soluble powder:water) to obtain a doxycycline hydrochloride aqueous solution, then add Maxingshigan oral liquid at a ratio of 1mL:1L (Maxingshigan oral liquid:doxycycline hydrochloride aqueous solution), mix well, and give the solution to sick chickens in drinking water for 5 consecutive days;

[0077] Experimental group 4: Dissolve 10% doxycycline hydrochloride soluble powder in water at a ratio of 1g:1L (10% doxycycline hydrochloride soluble powder:water) to obtain a doxycycline hydrochloride aqueous solution. Then add Shuanghuanglian oral liquid at a ratio of 1mL:1L (Shuanghuanglian oral liquid:doxycycline hydrochloride aqueous solution), mix well, and give the solution to sick chickens in drinking water for 5 consecutive days.

[0078] Experimental Group 5: The Chinese medicine preparation for treating poultry bronchial embolism provided in Comparative Example 1 of the present application was dissolved in water at a ratio of 1 mL: 2 L (Chinese medicine preparation: water), and the water was given to sick chickens for 5 consecutive days;

[0079] Experimental Group 6: The Chinese medicine preparation for treating poultry bronchial embolism provided in Comparative Example 2 of the present application was dissolved in water at a ratio of 1 mL: 2 L (Chinese medicine preparation: water), and the water was given to sick chickens for 5 consecutive days;

[0080] Control group: no medication.

[0081] Observation of general symptoms: During the experiment, the disease progression of each group of experimental chickens was recorded, and the mental state, appetite, and respiratory conditions of the sick chickens were observed.

[0082] Efficacy Evaluation: Dead chickens were immediately autopsied. Chickens showing typical lesions were considered dead. The number of dead chickens in each group during the entire trial was counted, and the mortality rate was calculated. At the end of the trial, surviving chickens in each group were autopsied and their organ lesions were observed. The number of cured, effective, and ineffective chickens was counted, and the cure rate, effective rate, and ineffective rate were calculated.

[0083] Cure: After administration, the patient's spirit, eating and drinking habits basically return to normal, respiratory symptoms disappear, and no caseous emboli are found in the bronchi and lungs during autopsy, which is considered a cure.

[0084] Effective: After administration, the patient's spirit, feeding and drinking habits are significantly improved, respiratory symptoms are alleviated or disappear, and autopsy shows a small amount of mild congestion, bleeding, inflammatory exudate and other lesions in the bronchi and lungs, but there is no obvious caseous embolism, which is considered effective.

[0085] Ineffective: After administration, if symptoms do not improve or even death occurs, and autopsy shows a large amount of caseous embolism in the bronchi and lungs, the drug is judged as ineffective.

[0086] The test results are as follows:

[0087] Observation of general symptoms: After administration, the number of mental depression in Experimental Group 1, Experimental Group 2, Experimental Group 3, Experimental Group 4, Experimental Group 5 and Experimental Group 6 was significantly decreased compared with the control group at 4-48 hours, and the specific data are shown in Table 1; after administration, the number of respiratory disorders in Experimental Group 1, Experimental Group 2, Experimental Group 3, Experimental Group 4, Experimental Group 5 and Experimental Group 6 was significantly decreased compared with the control group at 4-48 hours, and the specific data are shown in Table 2; after administration, the feed intake of Experimental Group 1 and Experimental Group 2 gradually increased after 24 hours and returned to normal after 48 hours; after administration, the feed intake of Experimental Group 3, Experimental Group 4, Experimental Group 5 and Experimental Group 6 gradually increased after 24 hours and was close to 80% of the normal feed intake after 48 hours.

[0088] Efficacy evaluation: After administration, the cure rate and effectiveness rate of Test Group 1, Test Group 2, Test Group 3, Test Group 4, Test Group 5 and Test Group 6 were significantly higher than those of the control group, and the cure rate of Test Group 1 and Test Group 2 was better than that of Test Group 3, Test Group 4, Test Group 5 and Test Group 6; the inefficiency rate and mortality rate of Test Group 1, Test Group 2, Test Group 3, Test Group 4, Test Group 5 and Test Group 6 were significantly lower than those of the control group, and the inefficiency rate and mortality rate of Test Group 1 and Test Group 2 were lower than those of Test Group 3, Test Group 4, Test Group 5 and Test Group 6. The specific data are shown in Table 3.

[0089] Table 1 Mental status of chickens in each experimental group

[0090]

[0091] Table 2 Respiratory status of chickens in each experimental group

[0092]

[0093] Table 3 Evaluation of therapeutic efficacy

[0094]

[0095] 2. Large-group treatment trials

[0096] Experimental animals: 24-day-old AA white-feathered broiler chickens raised in a broiler farm in Dalian, Liaoning.

[0097] Symptoms: Large groups of chickens begin to grunt, stretch their necks and open their mouths to breathe (abdominal breathing), make strange noises, and their feces are yellow-brown and thin; their feed intake decreases, and seemingly healthy chickens suddenly scream and die in the supine position.

[0098] Pathological autopsy: The autopsy showed that the trachea of ​​the dead chickens was congested and hemorrhagic, with white sticky sputum in the trachea, and bloody sputum in severe cases. There was yellow-white cheesy embolism in the lower end of the trachea to the bronchi, the lungs were congested and black, some lungs were edematous, and some had yellow-white block-like objects. The chest and abdominal air sacs were turbid and thickened, with foamy fibrinous exudates, and in severe cases, yellow cheesy objects were attached to the chest and abdominal air sacs.

[0099] Experimental grouping: The diseased chickens were divided into 6 groups according to the building of the chicken house where they were located, with one group in each building and about 32,000 chickens in each group. Each group was fed the same feed, and the rest of the feeding and management were the same except for the different medications.

[0100] Experimental drugs: the traditional Chinese medicine preparation for treating poultry bronchial embolism provided in Example 1 of the present application, the traditional Chinese medicine preparation for treating poultry bronchial embolism provided in Example 2 of the present application, the traditional Chinese medicine preparation for treating poultry bronchial embolism provided in Comparative Example 1 of the present application, the traditional Chinese medicine preparation for treating poultry bronchial embolism provided in Comparative Example 2 of the present application, 10% doxycycline hydrochloride soluble powder, Ma Xing Shi Gan oral liquid and Shuang Huang Lian oral liquid. All the drugs used are produced by Hebei Zhengyu Pharmaceutical Co., Ltd.

[0101] Test treatment:

[0102] In one experiment, the Chinese medicine preparation for treating poultry bronchial embolism provided in Example 1 of the present application was dissolved in water at a ratio of 1 mL: 2 L, and the water was given to sick chickens for 5 consecutive days.

[0103] Experimental Group 2: The Chinese medicine preparation for treating poultry bronchial embolism provided in Example 2 of the present application was dissolved in water at a ratio of 1 g: 2 L, and the water was given to sick chickens for 5 consecutive days;

[0104] In the third trial, 10% doxycycline hydrochloride soluble powder was dissolved in water at a ratio of 1g:1L, and then Ma Xing Shi Gan oral liquid was added at a ratio of 1mL:1L. The mixture was evenly mixed and given to sick chickens in drinking water for 5 consecutive days.

[0105] Experimental group 4: Dissolve 10% doxycycline hydrochloride soluble powder in water at a ratio of 1g:1L, then add Shuanghuanglian oral liquid at a ratio of 1mL:1L, mix well, and give the solution to sick chickens in drinking water for 5 consecutive days.

[0106] Experimental Group 5: The Chinese medicine preparation for treating poultry bronchial embolism provided in Comparative Example 1 of the present application was dissolved in water at a ratio of 1 mL: 2 L, and the water was given to sick chickens for 5 consecutive days;

[0107] Experimental group 6: The Chinese medicine preparation for treating poultry bronchial embolism provided in Comparative Example 2 of the present application was dissolved in water at a ratio of 1 mL: 2 L, and the solution was given to sick chickens as drinking water for 5 consecutive days.

[0108] Test indicators: Observe the appetite, disease progression, etc. of each group of test chickens every day, record the daily mortality rate of each group, perform autopsies before and after the test, and randomly select 20 dead chickens from each group for autopsy to observe the recovery of the respiratory tract.

[0109] Test results: See Table 4 for details.

[0110] Table 4 Results of large-group treatment trials

[0111]

[0112] According to Table 4, it can be seen that the first test group (the Chinese medicine oral liquid for treating poultry bronchial embolism provided in Example 1 of the present application) and the second test group (the Chinese medicine granules for treating poultry bronchial embolism provided in Example 2 of the present application) were significantly effective in treating bronchial embolism in chickens. After treatment, the respiratory symptoms were significantly alleviated, there was no embolism in the bronchi, the daily mortality rate was significantly reduced, and there was no significant difference between the oral liquid and the granules. Moreover, the therapeutic effects were better than those of the combination of antibiotics and Maxingshigan oral liquid, the combination of antibiotics and Shuanghuanglian oral liquid, and the Chinese medicine preparations provided in the comparative example.

[0113] In order to further explore the optimal use effect of the Chinese medicine preparation provided by this application, this application also conducted the following experiments:

[0114] 3. Effect Test 1

[0115] Experimental animals: 35-day-old Muscovy ducks from a Muscovy duck farm in Anhui Province.

[0116] Symptoms: Mild respiratory symptoms, which were not taken seriously and treated promptly, led to decreased feed intake, lethargy, gasping with open mouth and neck, strange screams, and finally death from suffocation.

[0117] Pathological autopsy: The trachea and bronchial walls of the dead duck were congested and hemorrhagic, with yellow cheesy substances blocking them. The lungs were black and edematous, and the air sacs were turbid and thickened.

[0118] Experimental grouping: The diseased ducks were divided into 5 groups according to the building of the duck house where they were located, with each building containing 1 group, and each group containing about 3000 ducks. Each group was fed the same feed and the rest of the feeding and management was the same except for the different medications.

[0119] Experimental drugs: the traditional Chinese medicine preparation for treating poultry bronchial embolism provided in Example 2 of the present application, the traditional Chinese medicine preparation for treating poultry bronchial embolism provided in Comparative Example 1 of the present application, the traditional Chinese medicine preparation for treating poultry bronchial embolism provided in Comparative Example 2 of the present application, 10% tilmicosin soluble powder and Maxingshigan oral liquid. All the drugs used are produced by Hebei Zhengyu Pharmaceutical Co., Ltd.

[0120] Test treatment:

[0121] In one test group, the Chinese medicine preparation for treating poultry bronchial embolism provided in Example 2 of the present application was evenly mixed into the feed at a ratio of 1 g:1 kg and fed for 5 consecutive days;

[0122] Group 2: 10% Tilmicosin soluble powder was evenly mixed into the feed at a ratio of 1g:1kg and fed for 5 consecutive days; at the same time, Maxing Shigan oral liquid was dissolved in water at a ratio of 1mL:1L and drunk for 5 consecutive days;

[0123] Experimental group 3: 10% tilmicosin soluble powder and the Chinese medicine preparation for treating poultry bronchial embolism provided in Example 2 of the present application were evenly mixed into the feed at a ratio of 1g:1kg and fed for 5 consecutive days;

[0124] In the fourth experimental group, 10% tilmicosin soluble powder was evenly mixed into the feed at a ratio of 1g:1kg and fed for 5 consecutive days; at the same time, the Chinese medicine preparation for treating bronchial embolism provided in comparative example 1 was dissolved in water at a ratio of 1mL:2L and fed for 5 consecutive days;

[0125] Experimental group 5: 10% tilmicosin soluble powder was evenly mixed into the feed at a ratio of 1g:1kg and fed for 5 consecutive days; at the same time, the Chinese medicine preparation for treating poultry bronchial embolism provided in Comparative Example 2 was dissolved in water at a ratio of 1mL:2L and drunk for 5 consecutive days.

[0126] Test indicators: The mental state and disease progression of the test ducks were observed every day, and the daily mortality rate of each group was recorded. Autopsies were performed before and after the start of the test. All dead ducks were autopsied and the bronchial conditions were observed. The specific results are shown in Table 5.

[0127] Table 5 Test results 1

[0128]

[0129] The results showed that the third experimental group (the Chinese medicine preparation for treating poultry bronchial embolism provided in Example 2 + tilmicosin soluble powder) was significantly effective in treating duck bronchial embolism, and was superior to other experimental groups in reducing mortality and bronchial embolism rate, indicating that the application is more effective when used in combination with antibiotics.

[0130] 4. Effect Test 2

[0131] Experimental animals: 28-day-old 817 broiler chickens from a farm in Liaocheng, Shandong Province.

[0132] Symptoms: difficulty breathing, stretching the neck to gasp, strange cries, shaking noses, large groups of people are listless, and food intake decreases instead of increases.

[0133] Pathological autopsy: The bronchi and bronchioles of the dead chickens were blocked by yellow cheesy substances. Some chickens had bloody foam in the trachea, edema, redness and blackening of the lungs, turbidity and thickening of the air sacs, and bleeding in the duodenal lymph nodes of some dead chickens.

[0134] Experimental Grouping: The diseased chickens were divided into 7 groups according to their chicken houses, with one group per house and approximately 50,000 chickens in each group. Each group was fed the same feed and, except for the different medications, all other aspects of the feeding and management were the same.

[0135] Experimental drugs: the traditional Chinese medicine preparation for treating poultry bronchial embolism provided in Example 1 of the present application, the traditional Chinese medicine preparation for treating poultry bronchial embolism provided in Example 2 of the present application, the traditional Chinese medicine preparation for treating poultry bronchial embolism provided in Comparative Example 1 of the present application, the traditional Chinese medicine preparation for treating poultry bronchial embolism provided in Comparative Example 2 of the present application, 10% doxycycline hydrochloride soluble powder, and Ma Xing Shi Gan oral liquid. All the drugs used are produced by Hebei Zhengyu Pharmaceutical Co., Ltd.

[0136] Test treatment:

[0137] In one experiment, the Chinese medicine preparation for treating poultry bronchial embolism provided in Example 1 of the present application was dissolved in water at a ratio of 1 mL: 2 L, and the water was given to sick chickens for 5 consecutive days.

[0138] Experimental Group 2: The Chinese medicine preparation for treating poultry bronchial embolism provided in Example 2 of the present application was dissolved in water at a ratio of 1 g: 2 L, and the water was given to sick chickens for 5 consecutive days;

[0139] In the third trial, 10% doxycycline hydrochloride soluble powder was dissolved in water at a ratio of 1g:1L, and then Ma Xing Shi Gan oral liquid was added at a ratio of 1g:1L. The mixture was evenly mixed and given to sick chickens in drinking water for 5 consecutive days.

[0140] In the fourth test group, 10% doxycycline hydrochloride soluble powder was dissolved in water at a ratio of 1 g:1 L, and the Chinese medicine preparation for treating poultry bronchial embolism provided in Example 1 of the present application was added at a ratio of 1 mL:2 L. The mixture was mixed evenly and the mixture was given to sick chickens in drinking water for 5 consecutive days.

[0141] In the fifth test group, 10% doxycycline hydrochloride soluble powder was dissolved in water at a ratio of 1 g:1 L, and the Chinese medicine preparation for treating bronchial embolism provided in Example 2 of the present application was added at a ratio of 1 g:2 L. The mixture was fully dissolved and given to sick chickens in drinking water for 5 consecutive days.

[0142] Experimental Group 6: 10% doxycycline hydrochloride soluble powder was dissolved in water at a ratio of 1 g: 1 L, and then the Chinese medicine preparation for treating poultry bronchial embolism provided in Comparative Example 1 of the present application was added at a ratio of 1 mL: 2 L, mixed evenly, and given to sick chickens in drinking water for 5 consecutive days;

[0143] Experimental group seven: 10% doxycycline hydrochloride soluble powder was dissolved in water at a ratio of 1 g: 1 L, and then the Chinese medicine preparation for treating bronchial embolism provided in Comparative Example 2 of the present application was added at a ratio of 1 mL: 2 L, mixed evenly, and given to sick chickens in drinking water for 5 consecutive days.

[0144] Test indicators: Observe the feeding, mental state, disease progression, etc. of the test chickens every day, record the daily mortality rate of each group, and perform autopsies before and after the test. Randomly select 20 dead chickens from each group for autopsy. If there are less than 20 chickens, all of them will be autopsied to observe the recovery of the respiratory tract.

[0145] Test results: as shown in Table 6.

[0146] Table 6 Test results 2

[0147]

[0148] As can be seen in Table 6, the Chinese herbal preparations for treating poultry bronchial embolism provided in the Examples of the present application were significantly effective in treating bronchial embolism in chickens, either alone or in combination with doxycycline. Mortality and bronchial embolism rates were significantly reduced, and feed intake and mental state recovered well. The combination with doxycycline significantly outperformed the other experimental groups. This is because doxycycline has antibacterial and antimycoplasmal effects, and the Chinese herbal preparations of the present application clear heat and promote lung function, resolve phlegm and relieve cough, cool blood and stop bleeding, eliminate carbuncle and discharge pus, and awaken the mind and stimulate the mind, resulting in a synergistic effect.

[0149] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. Any modifications, equivalent replacements or improvements made within the spirit and principles of the present application should be included in the scope of protection of the present application.

Claims

1. A Chinese medicine composition for treating poultry bronchial embolism, characterized in that: The invention is composed of the following raw material components in parts by weight: 15-30 parts of golden buckwheat, 8-15 parts of honeysuckle, 8-12 parts of agrimony, 6-12 parts of bitter almonds, 6-10 parts of trichosanthes, 3-6 parts of borneol and 2-4 parts of liquorice.

2. A Chinese medicine preparation for treating poultry bronchial embolism, characterized in that: The main component is the traditional Chinese medicine composition for treating poultry bronchial embolism as claimed in claim 1.

3. The Chinese medicinal preparation for treating poultry bronchial embolism according to claim 2, characterized in that: The dosage form is any one of oral liquid or granules.

4. The method for preparing the Chinese medicine preparation for treating poultry bronchial embolism according to claim 2, characterized in that: The steps include: Step 1, weigh the components except borneol according to the designed ratio of raw material components, add water and boil for 2-3 times, filter, and concentrate under reduced pressure until the relative density of the liquid is 1.02-1.06, and cool to obtain a concentrated solution; Step 2, dissolving borneol in alcohol to obtain a borneol alcohol solution; adding an emulsifier to the borneol alcohol solution and mixing well to obtain a borneol mixed solution; Step 3: Evenly mix the concentrated solution and the borneol mixed solution, add a preservative, add water to make the drug-liquid ratio 0.9-1:1-1.1, sterilize at 120° C.-122° C., and obtain the Chinese medicine preparation for treating poultry bronchial embolism.

5. The method for preparing the Chinese medicine preparation for treating poultry bronchial embolism according to claim 4, characterized in that: In step 1, the amount of water added is 8-14 times the weight of the drug; and / or In step 2, the mass ratio of borneol to alcohol is 1:10-30; and / or In step 2, the alcohol is ethanol with a mass concentration of 90%-95%; and / or In step 2, the emulsifier is Tween 80; and / or In step 2, the mass ratio of the emulsifier to the borneol solution is 1-3:

100.

6. The method for preparing the Chinese medicine preparation for treating poultry bronchial embolism according to claim 4, characterized in that: In step 3, the preservative is one or both of sodium benzoate and ethylparaben; and / or In step 3, the amount of the preservative added is 0.5wt%-0.6wt% of the total amount of the concentrate and the borneol mixture; and / or In step three, the sterilization time is 15min-20min.

7. The method for preparing the Chinese medicine preparation for treating poultry bronchial embolism according to claim 2, characterized in that: The steps include: S1. Weigh the components except borneol according to the designed ratio of raw material components, add water and boil for 2-3 times, filter, concentrate under reduced pressure, take samples, cool the samples to 60°C-65°C to measure the relative density, stop the reduced pressure concentration when the relative density is 1.08-1.25, cool, and obtain a concentrated extract; S2, weighing auxiliary materials, adding the concentrated extract, mixing evenly, granulating, and drying to obtain granules; S3. Dissolving borneol in alcohol, spraying the mixture on the surface of the particles, and drying the mixture to obtain the Chinese medicine preparation for treating poultry bronchial embolism.

8. The method for preparing the Chinese medicine preparation for treating poultry bronchial embolism according to claim 7, characterized in that: In S2, the auxiliary materials are powdered sugar and dextrin in a mass ratio of 3-10:

1.

9. The method for preparing the Chinese medicine preparation for treating poultry bronchial embolism according to claim 7, characterized in that: In S2, the amount of the auxiliary material is 3-6 times that of the concentrated extract.

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