Multi-point fluorescence detection apparatus and sample detection system

Abstract

Provided are a multi-point fluorescence detection apparatus (1) and a sample detection system. The multi-point fluorescence detection apparatus (1) comprises an optical module, which is arranged at a position corresponding to an optical fiber matrix (11), and provides an excitation light signal for an optical fiber module, wherein the optical fiber module receives the excitation light signal emitted by the optical module and transmits same to a plurality of areas to be subjected to detection, and separately transmits, to a collection module, reflected signals generated by means of fluorescence reactions in the plurality of areas to be subjected to detection; and the collection module is configured to collect reflected light signals, and transmit same to a remote terminal. The sample detection system comprises a multi-point fluorescence detection apparatus (1), a sample reaction apparatus (2) and a microfluidic push-injection sample adding apparatus (3), wherein the microfluidic push-injection sample adding apparatus (3) adds a sample to the sample reaction apparatus (2) for reaction, and after the reaction is completed, fluorescence detection for a sample reaction result is realized by means of the multi-point fluorescence detection apparatus (1). The sample detection system involves a more compact structure and facilitates installation and popularization.

Description

Multi-point fluorescence detection device and sample detection system Technical Field

[0001] The present application relates to a multi-point fluorescence detection device and a sample detection system, and relates to the field of biological detection technology. Background Art

[0002] Currently, most nucleic acid detection analyzers on the market (such as PCR instruments and constant-temperature amplification analyzers) often use single-tube, 8-tube strips, and 96-well plates as their supporting consumables. Therefore, the fluorescence detection schemes of these instruments require that the sampling route of the fluorescence detection module be a regular route. Some instruments that perform fluorescence detection on the side of the consumables (such as single-tube or 8-tube strips) must also require the detection route to be a single straight path and cannot perform fluorescence detection along a curved path. In addition, the detection position is mostly top detection, which requires that the consumables should be inserted from the top of the instrument, which is not conducive to the stacking design of the instrument.

[0003] Currently, a large number of institutions in the market are engaged in the research, development, and sales of POCT test kits and supporting instruments. Various fluorescent reaction areas are designed on their test kits. However, due to the limitations of existing fluorescence detection schemes, the test kits have to be designed as a single fluorescent reaction area (such as Cepheid's GeneXpert tester and Lifereal's Lifeready series analyzers), or multiple fluorescent reaction areas are designed on the same straight line (such as Ustar's UC series analyzers and GIAGEN's QIAstat-DX®Analyzer). Because if multiple fluorescent reaction areas are designed in an area with a non-straight path (such as disc-shaped, cylindrical or other test kits containing irregular curved path detection points), the fluorescence detection device in the instrument needs to move in multiple dimensions, which greatly increases the design difficulty and product cost of the instrument.

[0004] Furthermore, in the testing of trace samples, the microfluidic technology platform typically relies on microfluidic chips. Because commonly used microfluidic chips can only store small amounts of solution, the microfluidic structures within these chips struggle to transfer larger volumes (e.g., over 100 μL) and cannot achieve multiple levels of liquid volume transfer. Existing booster devices for liquid transfer typically utilize cylinders for boosting, resulting in large volumes and incompatibility with microfluidic technology platforms. SUMMARY OF THE INVENTION

[0005] The present application provides a multi-point fluorescence detection device and a sample detection system, wherein the multi-point fluorescence detection device includes: an optical module, which is arranged at a position corresponding to the optical fiber matrix and provides an excitation light signal to the optical fiber module; the optical fiber module receives the excitation light signal emitted by the optical module and transmits it to multiple areas to be detected, and transmits the reflection signals generated by the fluorescence reactions of the multiple areas to be detected to the acquisition module respectively; the acquisition module is used to collect the reflection light signal and transmit it to a remote terminal. The sample detection system includes a multi-point fluorescence detection device, a sample reaction device and a microfluidic push injection device. The microfluidic push injection device adds the sample to the sample reaction device for reaction. After the reaction is completed, the multi-point fluorescence detection device is used to realize fluorescence detection of the sample reaction result. The sample detection system of the present application has a more compact structure and is easy to install and promote. Technical issues

[0006] The object of the present invention is to provide a fluorescence detection device comprising a plurality of non-straight path detection points and a movable module, and a sample detection system comprising the fluorescence detection device. Technical Solutions

[0007] In order to achieve the above-mentioned invention objectives, the technical solutions adopted in this application are:

[0008] In a first aspect, the present application provides a multi-point fluorescence detection device, comprising:

[0009] The optical module is arranged at a position corresponding to the optical fiber matrix and provides an excitation light signal to the optical fiber module;

[0010] The optical fiber module receives the excitation light signal emitted by the optical module and transmits it to multiple areas to be detected, and transmits the reflection signals generated by the fluorescence reaction of the multiple areas to be detected to the acquisition module respectively;

[0011] The acquisition module is used to collect reflected light signals and transmit them to a remote terminal.

[0012] Furthermore, the optical fiber module includes a plurality of optical fiber bundles, one end of the plurality of optical fiber bundles are integrated together to form an optical fiber matrix, and the optical fiber ends at the other end are respectively set at positions corresponding to the respective fluorescent reaction areas.

[0013] Furthermore, the optical fiber bundles are independent of each other.

[0014] Furthermore, the optical module includes a box body, a light source, a lens, a first filter, a second filter and a dichroic mirror. The interior of the box body is hollow, the lens is connected to the interior of the box body, and divides the interior of the box body into a first cavity and a second cavity. A light source is provided on the side of the first cavity opposite to the lens, and a dichroic mirror is connected to the second cavity with an angle of 45°. The light emitted by the light source passes through the lens to form parallel light, which is refracted by the dichroic mirror and becomes downward excitation light; the upper and lower parts of the second cavity are provided with through holes for light to pass through, and a second filter is provided at the upper through hole. The first filter is provided on the side of the lens close to the second cavity.

[0015] Furthermore, the acquisition module includes an industrial camera 31 and a macro lens 32 .

[0016] Furthermore, the industrial camera captures an image of the reflected light signal and transmits it to a remote terminal via a wired or wireless method, and the remote terminal analyzes and processes the image.

[0017] Furthermore, it also includes a motion module, which is used to drive the optical fiber module and the area to be detected to move, so that the optical fiber module matches the optical module and realizes smooth transmission of the optical signal.

[0018] Furthermore, the motion module includes a slide rail, a slider, a motion base and a driving device. The slider is connected to the motion base and moves on the slide rail with the slider. The slider moves back and forth on the slide rail driven by the driving device; one side of the motion base is fixedly connected to the optical fiber matrix, and the other side is provided with a detection area. The light end of each optical fiber bundle is fixed at a position on the motion chassis corresponding to a fluorescence detection area.

[0019] Furthermore, the driving device is connected to the slider via a belt transmission structure.

[0020] In a second aspect, the present application further provides a sample detection system, which includes the above-mentioned fluorescence detection device.

[0021] Furthermore, the sample detection system includes a sample reaction device, a microfluidic push injection device and the multi-point fluorescence detection device. The microfluidic push injection device adds the sample to the sample reaction device for reaction. After the reaction is completed, the multi-point fluorescence detection device is used to realize fluorescence detection of the sample reaction results.

[0022] The sample reaction device includes a detection chassis and an amplification module. The detection chassis is provided with multiple fluorescence detection areas. The amplification module includes a heating device and a magnetic attraction device that can heat the liquid in the test box and adsorb the magnetic beads.

[0023] The microfluidic injection device comprises:

[0024] Connectors;

[0025] A vertical lifting system, which is connected to the connector and drives the connector to move up and down in a vertical direction;

[0026] A horizontal rotation system, which is connected to the connector and drives the connector to rotate clockwise or counterclockwise on a horizontal plane;

[0027] The multi-speed push injection component is connected to the microfluidic interface through a connector, and the push injection of liquids at different gears is achieved through the movement of the piston in the multi-speed push injection component.

[0028] Furthermore, the vertical lifting system includes a first screw motor, a lifting platform and a guide slide rod. The lifting platform is provided with a through hole matching the guide slide rod. The guide slide rod passes through the through hole. The lifting platform moves vertically back and forth along the guide slide rod driven by the first screw motor; the connector is connected to the lifting platform.

[0029] Furthermore, the horizontal rotation system includes a stepper motor, a driving wheel, a driven wheel and a belt. The stepper motor is connected to the driving wheel and drives the driving wheel to rotate. The driven wheel is connected to the connector. The belt is sleeved on the driving wheel and the driven wheel. The driving wheel drives the driven wheel and the connector to perform horizontal rotation through the belt, so that the connector drives the microfluidic interface to rotate clockwise or counterclockwise. The stepper motor, the axle of the driving wheel and the axle of the driven wheel are all connected to the lifting platform.

[0030] Furthermore, the multi-speed injection device includes a second screw motor, a guide rail, and a piston. The connector is provided with a hollow piston chamber, and the piston reciprocates in the piston chamber under the drive of the second screw motor. The lower end of the piston chamber is connected to the microfluidic interface.

[0031] Furthermore, a sealing ring is provided between the connector and the microfluidic interface.

[0032] Furthermore, the microfluidic injection device also includes a position sensor, which is connected to the controller or remote terminal and is used to determine the position of each motor, so as to facilitate real-time calibration of the motor accuracy during program operation.

[0033] Furthermore, the sample detection system further includes an outer frame bracket, and the guide slide rod and the lead screw motor are both connected to the outer frame bracket. Beneficial effects

[0034] Due to the adoption of the above technical solution, this application has the following characteristics:

[0035] 1. Unlike most nucleic acid detection analyzers (PCR instruments) or fully automatic nucleic acid detection analyzers (POCT) on the market, since the optical components, fiber optic components, and collection components are all fixed modes, the supporting consumables have to be inserted from the top of the instrument. Such a design is not conducive to the stacking design of the instrument. This application adds a motion module so that the device can be extended from the area to be detected on the device to install the consumables to be tested (such as reagents in the detection chassis, etc.), and during the movement, it can ensure that the position between the optical components, fiber optic components, and collection components basically does not change. In this way, the installation method of the consumables can be changed to side installation (not top installation), and the performance of fluorescence collection can be guaranteed not to be affected. At the same time, in conjunction with the microfluidic booster loading device that can only be set above the consumables (detection chassis), the height of the entire system is reduced, making it more convenient to use.

[0036] 2. Currently, the airflow drive cylinder of most microfluidic chips on the market is a separate component. This application uses a connector, a vertical lifting system, a horizontal rotation system, and a multi-speed injection system to complete the liquid transfer drive, and does not require an additional space for placing the cylinder.

[0037] 3. In this application, the cylinder piston is in the microfluidic interface and no additional cylinder accessories are required.

[0038] 4. The cylinder of common microfluidic technology requires an air tube to be connected to the microfluidic interface, and the air tube cannot rotate indefinitely; however, in this application, the cylinder is built into the microfluidic interface and can rotate indefinitely following the horizontal rotation system and the microfluidic interface, enabling multi-directional liquid transfer.

[0039] 5. The through holes of the piston and connector of the present application are both segmented in design. Different segmented stages combined with different diameter designs can meet the requirements of different liquid volumes in multiple gears.

[0040] 6. Combined with the above features, the sample detection system of this application has a more compact structure and is easy to install and promote. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Various other advantages and benefits will become apparent to those skilled in the art upon reading the detailed description of the preferred embodiment below. The accompanying drawings are for illustration purposes only and are not to be considered as limiting the present application. Throughout the drawings, the same reference numerals are used to represent the same components. In the drawings:

[0042] FIG1 is a schematic structural diagram of a sample detection system according to an embodiment of the present application;

[0043] FIG2 is a schematic structural diagram of a fluorescence detection device according to an embodiment of the present application;

[0044] FIG3 is a schematic structural diagram of an optical module according to an embodiment of the present application;

[0045] FIG4 is a schematic structural diagram of a chassis to be inspected according to an embodiment of the present application.

[0046] FIG5 is a schematic structural diagram of a microfluidic injection device according to an embodiment of the present application;

[0047] FIG6 is a schematic diagram of the internal structure of a connector and a piston in a microfluidic injection device according to an embodiment of the present application;

[0048] FIG7 is a schematic diagram of a liquid transfer device in a microfluidic injection sampling device according to an embodiment of the present application.

[0049] Description of reference numerals:

[0050] 1 Multi-point fluorescence detection device, 2 Sample reaction device, 3 Microfluidic injection and sample loading device; 11 Fiber optic matrix, 12 Fiber optic end, 13 Fiber optic bundle, 21 Box body, 22 Light source, 23 Lens, 24 First filter, 25 Second filter, 26 Dichroic mirror, 27 First cavity, 28 Second cavity, 31 Industrial camera, 32 Macro lens, 41 Slide rail, 42 Slider, 43 Motion base, 44 Belt drive structure, 5 Detection chassis, 51 Fluorescence detection area; 301 Connector, 302 vertical lifting system, 303 horizontal rotation system, 304 multi-speed injection system, 305 microfluidic interface, 306 sealing ring, 307 position sensor, 308 outer frame bracket, 309 first screw motor, 310 lifting platform, 311 guide slide, 312 stepper motor, 313 driving wheel, 314 driven wheel, 315 belt, 317 second screw motor, 318 guide rail, 319 piston, 320 tip component, 3101 bearing. Best Mode for Carrying Out the Invention

[0051] It should be understood that the terms used herein are for the purpose of describing specific example embodiments only and are not intended to be limiting. Unless the context clearly indicates otherwise, the singular forms "one", "an" and "said" as used herein may also be meant to include plural forms. The terms "comprise", "include", "contain" and "have" are inclusive and therefore specify the presence of stated features, steps, operations, elements and / or parts, but do not exclude the presence or addition of one or more other features, steps, operations, elements, parts, and / or combinations thereof. The method steps, processes, and operations described herein are not to be construed as necessarily requiring them to be performed in the specific order described or illustrated, unless the order of execution is clearly indicated. It should also be understood that additional or alternative steps may be used.

[0052] Although the terms first, second, third, etc. can be used in the text to describe multiple elements, components, regions, layers and / or sections, these elements, components, regions, layers and / or sections should not be limited by these terms. These terms can only be used to distinguish an element, component, region, layer or section from another region, layer or section. Unless the context clearly indicates otherwise, terms such as "first", "second" and other numerical terms do not imply order or sequence when used in the text. Therefore, the first element, component, region, layer or section discussed below can be referred to as the second element, component, region, layer or section without departing from the teaching of the example embodiments.

[0053] For ease of description, spatially relative terms may be used herein to describe the relationship of one element or feature relative to another element or feature as shown in the figures, such as "inside," "outside," "inner side," "outer side," "lower," "upper," etc. Such spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the figures.

[0054] Example 1

[0055] As shown in Figures 2-4, this embodiment provides a multi-point fluorescence detection device, including a fiber optic module, an optical module, a collection module, and a motion module:

[0056] The optical fiber module includes multiple optical fiber bundles 13, one end of which is integrated together to form an optical fiber matrix 11, and the optical fiber ends 12 at the other ends are respectively fixed to positions corresponding to each fluorescent reaction area on the chassis to be detected; since each optical fiber bundle can be installed independently (for example, on the moving base, it refers to the mounting hole corresponding to the optical fiber bundle, and is fixed on the side with a locking screw), each optical fiber bundle is independent of each other and can be used for the independent transmission of fluorescent signals from any irregularly distributed fluorescent reaction area.

[0057] The optical fiber module (optical fiber matrix end) receives the excitation light signal emitted by the optical module and transmits it to multiple areas to be detected, and transmits the reflection signals generated by the fluorescence reaction of the multiple areas to be detected to the acquisition module respectively;

[0058] The optical module is set at a position corresponding to the optical fiber matrix and provides an excitation light signal for the optical fiber module.

[0059] The optical module includes a housing 21, a light source 22, a lens 23, a first filter 24, a second filter 25, and a dichroic mirror 26. The interior of the housing is hollow, and the lens is connected to the interior of the housing, dividing the interior of the housing into a first cavity 27 and a second cavity 28. A light source is provided on the side of the first cavity opposite the lens, and a dichroic mirror is connected to the second cavity, which is tilted at 45 degrees. Light emitted by the light source passes through the lens to form parallel light, which is refracted by the dichroic mirror and converted into downward excitation light. The second cavity is provided with through holes at the top and bottom for light to pass through. A second filter is provided at the upper through hole. The side of the lens closest to the second cavity is provided with a first filter. When the device is in the detection state, the through hole below the second cavity is located above the fiber matrix. In this case, the excitation light can be transmitted through this through hole to the fiber matrix, and then transmitted to each fluorescence reflection area through each fiber bundle. Under the illumination of the excitation light signal, the fluorescence reaction area generates a reflected light signal, which is then transmitted to the fiber matrix through the fiber bundle, and then transmitted through the dichroic mirror and second filter to the collection module above.

[0060] The acquisition module is used to collect reflected light signals and transmit them to a remote terminal. The acquisition module includes an industrial camera 31 and a macro lens 32. The industrial camera captures images of the reflected light signals and transmits them to the remote terminal via a wired or wireless connection. The remote terminal then analyzes and processes the images. Because the acquisition module can simultaneously capture reflected light signals from multiple fiber bundles in the fiber matrix, information acquisition efficiency is superior to that of conventional single-point fluorescence detection methods.

[0061] The motion module is used to drive the optical fiber module and the unit to be detected to move, so that the optical fiber module matches the optical module and ensures the smooth transmission of the optical signal.

[0062] The motion module includes a slide rail 41, a slider 42, a motion base 43, and a drive mechanism. The slider is connected to the motion base and moves along the rail. Driven by the drive mechanism, the slider reciprocates along the rail. One side of the motion base is fixedly connected to the fiber matrix, while the other side is provided with the detection area. The optical end of each fiber bundle is fixed to a position on the motion base corresponding to a fluorescence detection area.

[0063] The driving device may be a motor, and the motor may be connected to the slider via a belt transmission structure.

[0064] The fluorescence detection area can be used in conjunction with a detection chassis. As shown in FIG4 , the detection chassis 5 includes a plurality of fluorescence detection areas 51 .

[0065] During use, the motor is driven, and the slider drives the moving base to move on the slide rail, so that the area to be detected moves to the outside of the device, and multiple samples to be detected are placed in the fluorescence detection area 51 respectively. The motor is driven to make the moving base move inside the device until the optical fiber matrix matches the through hole at the lower end of the optical module to ensure the smooth transmission of the light signal. Turn on the light source, the fluorescence detection area undergoes a fluorescence reaction, an industrial camera is used to take a picture, and the image is transmitted to a remote terminal. At this time, since each optical fiber bundle can be installed independently (for example, on the moving base, it refers to the mounting hole corresponding to the optical fiber bundle, and is fixed on the side with a tight screw), each optical fiber bundle is independent of each other and can be used for the separate and independent transmission of fluorescence signals in any irregularly distributed fluorescence reaction area. The acquisition module can simultaneously collect the reflected light signals transmitted by multiple optical fiber bundles in the optical fiber matrix, so the information acquisition efficiency is higher than the common single-point fluorescence detection mode.

[0066] Example 2

[0067] As shown in Figures 1-7, this embodiment provides a sample detection system, including the multi-point fluorescence detection device 1 described in Example 1, a sample reaction device 2 and a microfluidic push injection device 3. The microfluidic push injection device is located above the sample reaction device and is used to add samples to the sample reaction device and realize liquid transfer in the sample reaction device; the multi-point fluorescence detection device is located on one side of the sample reaction device and is used to realize fluorescence detection of the sample reaction results after the reaction is completed.

[0068] The sample reaction device 2 includes a detection chassis and an amplification module. The detection chassis is provided with a plurality of fluorescence detection areas. The amplification module includes a heating device and a magnetic attraction device that can heat the liquid in the test box and adsorb the magnetic beads.

[0069] Furthermore, as shown in FIG5 , the microfluidic push injection device includes a connector 301 , a vertical lifting system 302 , a horizontal rotation system 303 , a multi-speed push injection system 304 , a microfluidic interface 305 , a sealing ring 306 , a position sensor 307 and an outer frame bracket 308 .

[0070] The connector 301 is provided with a through hole inside, and the diameter of the through hole decreases from top to bottom. The through hole in the connector 301 can be 2-3 segments (or more segments) of circular holes of different diameters, and the diameter of the circular holes decreases from top to bottom. In this way, the circular holes of different diameters can be matched with pistons of different sizes to achieve the transfer of liquid samples with different precision ranges. The connector can be made of metal or other polymer materials with high strength and long service life like metal. Generally speaking, metal is preferred. The lower end of the connector 301 is provided with a joint that matches the microfluidic interface 305. The cross-section of the joint (i.e., the outer surface of the entire lower end of the connector 301 that does not conflict with the circular through hole) is non-circular. The non-circularity refers to a non-circular shape that can meet the requirements of synchronous rotation after connection, such as square, semi-circular, hexagonal, etc. The lower end of the connector 301 is provided with a microfluidic interface that can drive the microfluidic interface to rotate horizontally after being connected to the microfluidic interface. The planar structure of the connection can also achieve a sealing effect with the cooperation of the sealing ring 306. As shown in Figure 6, when the connector at the lower end of the connector is inserted into the microfluidic interface 305, the sealing ring 306 made of elastic material is clamped between the connector 301 and the microfluidic interface 305 to achieve a sealing effect.

[0071] The vertical lifting system 302 comprises a first lead screw motor 309, a lifting platform 310, and a guide rod 311. The guide rod 311 is fixedly connected to the outer frame 308. Driven by the first lead screw motor 309, the lifting platform 310 reciprocates vertically along the guide rod 311. The lifting platform 310 is provided with a bearing 3101, and the connector 301 is fixed to the inner ring of the bearing 3101. The connector 301 can reciprocate vertically with the lifting platform 310 and can also rotate within the bearing about the central axis of the connector 301 under the drive of the horizontal rotation system 303. The reciprocating vertical motion of the connector 301 with the lifting platform 310 drives the tip assembly 320 with the microfluidic interface 305 to reciprocate vertically.

[0072] The horizontal rotation system 303, consisting of a stepper motor 312, a driving pulley 313, a driven pulley 314, and a belt 315, is used to drive the connector 301 to perform horizontal rotational motion. Specifically, the stepper motor 312 drives the driving pulley 313, which in turn drives the driven pulley 314 via the belt 315. The driven pulley 314 then drives the connected connector 301 to rotate. This allows the connector 301 to rotate the microfluidic interface 305 clockwise or counterclockwise (rotation is based on the connector's central axis; when a square connector is inserted vertically into the microfluidic interface, the connector can drive the microfluidic interface to rotate).

[0073] The multi-speed push injection system 304 comprises a second lead screw motor 317, a guide rail 318, and a piston 319. The second lead screw motor 317 propels the piston 319 inside the connector 301 up and down along the guide rail 318, thereby performing micro-control of airflow through the microfluidic interface 305. The piston 319 can be of varying diameters, matched to the inner diameter of the through hole in the connector 301. This matching of the two allows for push injections of varying precision levels. For example, a 10 μl micro-bolus injection can be performed using a thinner piston, allowing a relatively small amount of airflow to be delivered with the same piston travel distance. Similarly, a larger diameter piston can be used for boosting large-volume airflow control.

[0074] The microfluidic interface 305 of the present application has a groove at one end, the shape of which matches the shape of the connector 301, allowing the connector 301 to drive the microfluidic interface 305 to rotate. The other end of the microfluidic interface is connected to a liquid storage or transfer device. An example of a liquid transfer device in this embodiment is shown in Figure 7. The other end of the microfluidic interface 305 is connected to a pipette tip assembly 320. The pipette tip assembly 320 rotates clockwise or counterclockwise under the drive of the connector 301 via the microfluidic interface 305.

[0075] As a further solution of this embodiment, the microfluidic injection device may also include a position sensor 307. There may be two position sensors 307, which are respectively connected to the first screw motor and the second screw motor. They may be trigger switches such as optocouplers and micro switches, which are used to determine the position of each motor, so as to facilitate real-time calibration of the motor's accuracy during program operation. For example, the position sensor is generally installed on the running path of the moving part. When the motor drives the moving part to move, the contact installed on the moving part will contact the position sensor, and the position sensor will give a feedback signal to the processor (or remote terminal). At this time, the processor (or remote terminal) will automatically determine the specific position of the moving part. When there is a deviation between the number of motor running steps given by the processor (or remote terminal) and the number of steps output when the position sensor is actually triggered, the software will automatically compensate for the deviation to ensure the running accuracy of the moving part.

[0076] The outer frame bracket 308 is a standard rectangular parallelepiped frame that can be connected to the rack of the sample detection system.

[0077] During use, the microfluidic injection device is used to perform sample addition and liquid transfer in the detection chassis according to the following steps:

[0078] 1) Select an appropriate piston based on the volume of liquid to be transferred and connect the microfluidic interface to the liquid storage or transfer device;

[0079] 2) Driving the first screw motor to move the connector downward to height 1 and connect it to the microfluidic interface;

[0080] 3) Drive the progressive motor to rotate the liquid storage or transfer device to the position where the liquid is to be taken;

[0081] 4) Drive the first screw motor to drive the connector downward to height 2 (at this time the liquid extraction component extends into the liquid); drive the second screw motor to drive the piston to extract the liquid;

[0082] 5) Drive the first screw motor to drive the connector to move upward to height 1, drive the advance motor to drive the liquid storage or transfer device to rotate to the position to be discharged;

[0083] 6) Drive the first screw motor to drive the connector downward to height 2 (at this time, the liquid extraction component extends into the liquid container to be discharged); drive the second screw motor to drive the piston to push out the liquid;

[0084] 7) Drive the first screw motor to drive the connector to move upward to height 1 (at this time, the liquid taking component leaves the liquid container to be discharged), completing the liquid transfer.

[0085] The device in this embodiment controls the rotational speed and direction of the three motors in the device to control the suction head assembly to complete the three movements of descent, ascent, and rotation. The pressure generated by the piston movement is used to absorb and release the liquid, enabling the suction head assembly to transfer the solution between different areas of the test base.

[0086] After sample loading, the sample in the detection chassis completes the heating reaction or magnetic bead adsorption under the heating and / or magnetic attraction of the amplification module. Subsequently, the multi-point fluorescence detection device is used to capture images of the fluorescence detection area. Because each fiber optic bundle can be installed independently (for example, on the kinematic base, corresponding mounting holes are defined for the fiber optic bundles, and secured with side screws), each fiber optic bundle is independent of the others and can be used to independently transmit fluorescence signals from any irregularly distributed fluorescence reaction area. The acquisition module can simultaneously capture reflected light signals from multiple fiber optic bundles in the fiber matrix, resulting in higher information acquisition efficiency than conventional single-point fluorescence detection methods.

[0087] CROSS-REFERENCE TO RELATED APPLICATIONS

[0088] This application claims priority to the Chinese patent application filed on July 22, 2024 (application number 202410980781.9) and the Chinese patent application filed on August 7, 2023 (application number 202310987259.9), the entire contents of which are hereby incorporated by reference into this document. Industrial Applicability

[0089] Due to the adoption of the above technical solution, this application has the following characteristics:

[0090] 1. Unlike most nucleic acid detection analyzers (PCR instruments) or fully automatic nucleic acid detection analyzers (POCT) on the market, since the optical components, fiber optic components, and collection components are all fixed modes, the supporting consumables have to be inserted from the top of the instrument. Such a design is not conducive to the stacking design of the instrument. This application adds a motion module so that the device can be extended from the area to be detected on the device to install the consumables to be tested (such as reagents in the detection chassis, etc.), and during the movement, it can ensure that the position between the optical components, fiber optic components, and collection components basically does not change. In this way, the installation method of the consumables can be changed to side installation (not top installation), and the performance of fluorescence collection can be guaranteed not to be affected. At the same time, in conjunction with the microfluidic booster loading device that can only be set above the consumables (detection chassis), the height of the entire system is reduced, making it more convenient to use.

[0091] 2. Currently, the airflow drive cylinder of most microfluidic chips on the market is a separate component. This application uses a connector, a vertical lifting system, a horizontal rotation system, and a multi-speed injection system to complete the liquid transfer drive, and does not require an additional space for placing the cylinder.

[0092] 3. In this application, the cylinder piston is in the microfluidic interface and no additional cylinder accessories are required.

[0093] 4. The cylinder of common microfluidic technology requires an air tube to be connected to the microfluidic interface, and the air tube cannot rotate indefinitely; however, in this application, the cylinder is built into the microfluidic interface and can rotate indefinitely following the horizontal rotation system and the microfluidic interface, enabling multi-directional liquid transfer.

[0094] 5. The through holes of the piston and connector of the present application are both segmented in design. Different segmented stages combined with different diameter designs can meet the requirements of different liquid volumes in multiple gears.

[0095] 6. Combined with the above features, the sample detection system of this application has a more compact structure and is easy to install and promote.

Claims

1. A multi-point fluorescence detection device, characterized in that: include: An optical module is arranged at a position corresponding to the optical fiber matrix and provides an excitation light signal for the optical fiber module; The optical fiber module receives the excitation light signal emitted by the optical module and transmits it to a plurality of areas to be detected, and transmits the reflection signals generated by the fluorescence reaction of the plurality of areas to be detected to the collection module respectively; The acquisition module is used to collect reflected light signals and transmit them to the remote terminal.

2. The multi-point fluorescence detection device according to claim 1, characterized in that: The optical fiber module includes a plurality of optical fiber bundles, one end of which is integrated together to form an optical fiber matrix, and the optical fiber ends at the other end are respectively arranged at positions corresponding to each fluorescent reaction area.

3. The multi-point fluorescence detection device according to claim 2, characterized in that: The optical fiber bundles are independent of each other.

4. The multi-point fluorescence detection device according to claim 1, characterized in that: The optical module includes a box body, a light source, a lens, a first filter, a second filter and a dichroic mirror. The interior of the box body is hollow. The lens is connected to the interior of the box body and divides the interior of the box body into a first cavity and a second cavity. A light source is provided on the side of the first cavity opposite to the lens. A dichroic mirror is connected to the second cavity and is inclined at 45 degrees. Light emitted by the light source passes through the lens to form parallel light, which is refracted by the dichroic mirror and becomes downward excitation light. Through holes are provided above and below the second cavity for light to pass through. A second filter is provided at the upper through hole. A first filter is provided on the side of the lens close to the second cavity.

5. The multi-point fluorescence detection device according to claim 1, characterized in that: The acquisition module includes an industrial camera and a macro lens.

6. The multi-point fluorescence detection device according to claim 5, characterized in that: The industrial camera captures an image of the reflected light signal and transmits it to a remote terminal via a wired or wireless method, and the remote terminal analyzes and processes the image.

7. The multi-point fluorescence detection device according to any one of claims 1 to 6, characterized in that: It also includes a motion module, which is used to drive the optical fiber module and the unit to be detected to move, so that the optical fiber module matches the optical module and realizes smooth transmission of optical signals.

8. The multi-point fluorescence detection device according to claim 7, characterized in that: The motion module includes a slide rail, a slider, a motion base and a driving device. The slider is connected to the motion base and moves on the slide rail with the slider. The slider reciprocates on the slide rail driven by the driving device. One side of the motion base is fixedly connected to the optical fiber matrix, and the other side is provided with a detection area. The light end of each optical fiber bundle is respectively fixed at a position on the motion chassis corresponding to a fluorescent detection area.

9. The multi-point fluorescence detection device according to claim 8, characterized in that: The driving device is connected with the sliding block through a belt transmission structure.

10. A sample detection system, characterized in that: It comprises the multi-point fluorescence detection device described in any one of claims 1-9.

11. The sample detection system according to claim 10, characterized in that: It comprises a sample reaction device, a microfluidic injection sample adding device and the multi-point fluorescence detection device.

12. The sample detection system according to claim 11, characterized in that: The microfluidic injection device comprises: Connectors; A vertical lifting system, which is connected to the connector and drives the connector to move up and down in a vertical direction; A horizontal rotation system, which is connected to the connector and drives the connector to rotate clockwise or counterclockwise on a horizontal plane; The multi-speed push injection component is connected to the microfluidic interface through a connector, and the push injection of liquids at different gears is achieved through the movement of the piston in the multi-speed push injection component.

13. The sample detection system according to claim 12, characterized in that: The vertical lifting system includes a first screw motor, a lifting platform and a guide slide rod. The lifting platform is provided with a through hole matching the guide slide rod. The guide slide rod passes through the through hole. The lifting platform moves vertically back and forth along the guide slide rod driven by the first screw motor. The connector is connected to the lifting platform.

14. The sample detection system according to claim 12, characterized in that: The horizontal rotation system includes a stepper motor, a driving wheel, a driven wheel and a belt. The stepper motor is connected to the driving wheel and drives the driving wheel to rotate. The driven wheel is connected to the connector. The belt is sleeved on the driving wheel and the driven wheel. The driving wheel drives the driven wheel and the connector to perform horizontal rotation through the belt, so that the connector drives the microfluidic interface to rotate clockwise or counterclockwise. The stepper motor, the axle of the driving wheel, and the axle of the driven wheel are all connected to the lifting platform.

15. The sample detection system according to claim 12, characterized in that: The multi-speed injection system comprises a second screw motor, a guide rail and a piston. A hollow piston cavity is provided inside the connector. The piston reciprocates in the piston cavity under the drive of the second screw motor.

16. The sample detection system according to claim 12, characterized in that: A sealing ring is sandwiched between the connector and the microfluidic interface.

17. The sample detection system according to claim 12, characterized in that: The microfluidic injection device also includes a position sensor for determining the position of each motor, so as to facilitate real-time calibration of the motor accuracy during program operation.

18. The multi-directional and multi-position microfluidic injection device according to claim 12, characterized in that: The microfluidic injection and sample adding device further comprises an outer frame bracket, and the guide slide rod and the lead screw motor are both connected to the outer frame bracket.

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