Reagent disc and biochemical analyzer

Abstract

Disclosed in the present application are a reagent disc and a biochemical analyzer. The reagent disc comprises a disc body, the disc body of the reagent disc being provided with a ring operation region, and at least one sample cup, a plurality of reagent cups and a plurality of reaction cups being provided on the ring operation region along the ring-shaped track thereof. The biochemical analyzer comprises a reaction disc, a pipetting mechanism and an optical signal test mechanism, which are detachably connected to the reagent disc. Various operations required by a biochemical test are completed by means of the cooperation of the up-down movement of a pipetting tip of the pipetting mechanism and the rotation of the reaction disc. The optical signal test mechanism is used for performing optical signal analysis and test on a reagent or a mixture of a reagent and a sample in a reaction cup. The present application allows for rapid multi-item test of samples, has a simple instrument structure, involves simple operation and can generate accurate results.

Description

Reagent disk and biochemical analyzer Technical Field

[0001] The present application relates to the technical field of analysis and detection equipment, and in particular to a reagent disk and a biochemical analyzer. Background Art

[0002] Biochemical analysis refers to a combination of instruments and reagents that uses the principle of photoelectric colorimetry to detect and analyze specific biochemical components in samples. It can provide important information for the diagnosis, treatment, prevention of diseases and health status assessment in clinical practice, and is an essential basic equipment for medical testing.

[0003] In the related technologies, clinical biochemical analyzers are currently mostly operated manually or fully automatic. Among them, manual operations such as adding samples, adding reagents, mixing, and testing required for biochemical testing are completed by hand, which is inefficient and has defects such as human errors, which seriously limit its use scenarios. Fully automatic instruments automatically complete the operations required for biochemical testing such as adding samples, adding reagents, mixing, testing, cleaning reaction cups, and cleaning sample needles. They have the advantages of high detection efficiency and accurate test results. However, since they need to design sample addition, reagent addition, mixing, dilution, cleaning of liquid needles, cleaning of reaction cups, etc. into corresponding functional modules for implementation, they also have defects such as complex instrument structure, large size, high price, need for professional operation, and unsuitable for small sample testing. However, in the vast majority of primary medical institutions (rural health clinics, private clinics, grassroots communities, hospital bedside, emergency sites, etc.) and animal clinics, due to the fact that most medical staff are non-professional medical examiners, the number of test samples is small, the site is small and the funds are limited, there is an urgent need for analytical instruments with simple structure, compact size and easy operation to meet the needs of on-site rapid testing.

[0004] Especially in some application scenarios, it is necessary to use small equipment to perform rapid biochemical testing of multiple items on samples. Currently, the technology of dry chemical microfluidic biochemical reaction disk is applied to this scenario. This technology can simultaneously complete the detection of up to 20 biochemical items in the sample in more than ten minutes. It has the advantages of small instruments and easy operation. However, this technology has disadvantages such as poor repeatability of test results, complex reagent production process, low yield, and high cost, which limit its wide application in clinical practice.

[0005] Summary of the Invention

[0006] In order to provide a biochemical analyzer that can perform multiple rapid tests on samples simultaneously, has a simple instrument structure, is easy to operate, and produces accurate results, the present application provides a reagent tray and a biochemical analyzer.

[0007] The present application provides a reagent disk and a biochemical analyzer, which adopt the following technical solutions:

[0008] A reagent disc comprises a disc body, wherein the disc body is provided with a circular working area, and at least one sample cup, a plurality of reagent cups and a plurality of reaction cups are arranged on the circular working area along its circular track.

[0009] By adopting the above technical solution, the sample cup, reagent cup and reaction cup are integrated in the circular working area of ​​the disk body, so that the samples, reaction cups, first reagents and second reagents required for biochemical testing of various projects are integrated on a reagent disk. They can be flexibly controlled and used by rotating the reagent disk. At that time, the pipetting head of the biochemical analyzer only needs to move up and down to achieve the absorption of the corresponding reagents, so that the elements of biochemical testing are integrated, which greatly reduces the dependence on the mechanical structure of the instrument, simplifies the instrument structure, makes the instrument miniaturized, and simplifies the operation.

[0010] Preferably, the tray body is further provided with a sealing member, which is used to seal the reagent cup and the reaction cup and is sealedly connected to the reagent cup and the reaction cup.

[0011] By adopting the above technical solution, the sealing member seals the reagent cup and the reaction cup, which facilitates pre-filling of the reagent cup and the reaction cup with reagents and is beneficial to the storage and transportation of the pre-filled reagents.

[0012] Preferably, each of the reaction cups is pre-installed with a first reagent required for all biochemical test items preset on the reagent disk, the first reagent is a liquid reagent or a solid reagent, and the reaction cup is a transparent material cup.

[0013] By adopting the above technical solution, the reaction cup accommodates the first reagent required for each biochemical test respectively, and the reaction cup is the reaction container for each biochemical test item preset on the reagent tray, so that the biochemical test reaction is carried out in the reaction cup. The setting of combining the reagent container and the reaction container into one reduces the volume of the reagent tray. The reaction cup is made of a transparent material cup to facilitate optical detection of the reagents or mixtures of reagents and samples for the biochemical test items.

[0014] Preferably, each of the reagent cups is pre-installed with a second reagent required for all biochemical test items preset on the reagent disk, and the second reagent is a liquid reagent or a solid reagent.

[0015] By adopting the above technical solution, the reagent cup can accommodate each second reagent required for biochemical detection respectively. When any biochemical reaction requires its specific second reagent to participate in the reaction, the pipetting mechanism can transfer it quantitatively from the reagent cup to the reaction cup.

[0016] Preferably, the disk body is further provided with a liquid containing cup along its circular track in the circular working area, the liquid containing cup is pre-filled with cleaning liquid and / or diluent, and the liquid containing cup is sealed by the sealing member.

[0017] By adopting the above technical solution, the sealing member seals the liquid-containing cup, which is convenient for pre-filling the cleaning liquid and / or diluent in the liquid-containing cup, and is conducive to the storage and transportation of the pre-filled cleaning liquid and / or diluent. After the pipette head / pipette tip performs any operation of adding sample, adding reagent, and mixing, before moving to the next operation, in order to avoid cross infection, the pipette head / pipette tip can be cleaned in the cleaning liquid before performing the next operation of adding sample, adding reagent, and mixing; when the sample needs to be diluted, the sample diluent can be pre-filled in the liquid-containing cup, and the sample and diluent can be quantitatively aspirated and mixed by the pipette head / pipette tip; when the reagents in the reaction cup and the reagent cup are pre-embedded in a solid form, the diluent can be pre-filled in the liquid-containing cup, and the diluent can be quantitatively aspirated by the pipette head / pipette tip on the reagent disk before testing and added to each reaction cup and reagent cup to re-dissolve the solid reagent before performing biochemical testing.

[0018] Preferably, the disk body is further provided with a suction head position along its circular track in the circular working area, and the suction head position is used for placing the suction head.

[0019] By adopting the above technical solution, the pipette tip is placed in the pipette tip position, and the pipette tip position is arranged along the circular track of the circular working area, so that the pipette tip can be assembled on the pipette head for transferring reagents.

[0020] Preferably, the sealing member is any one of an aluminum foil sealing film, a silicone sealing film, a plastic sealing film and a rubber sealing film.

[0021] By adopting the above technical solution, the sealing member is any one of an aluminum foil sealing film, a silicone sealing film, a plastic sealing film and a rubber sealing film, which is conducive to ensuring the sealing. At the same time, the aluminum foil sealing film, the silicone sealing film, the plastic sealing film and the rubber sealing film are economical and practical, and are conducive to manually tearing or punching the reagent disc with a punching device before use.

[0022] Preferably, the disc body is provided with a connection structure for detachable connection with a biochemical analyzer.

[0023] By adopting the above technical solution, the disc body is installed on the reaction disc of the biochemical analyzer through the connecting structure to perform biochemical item detection operations.

[0024] Preferably, the reagent disc is integrally formed.

[0025] The tray body and various cup shapes are integrally molded by injection molding, which is beneficial to improving the consistency and production efficiency of the reagent tray and making the reagent tray convenient for disposable use.

[0026] A biochemical analyzer includes a reaction disk, a pipetting mechanism, and an optical signal detection mechanism. The reaction disk is arranged to rotate horizontally and is provided with a mounting portion for detachably connecting to the disk body of the above-mentioned reagent disk. The optical signal detection mechanism is used to perform optical signal detection on the reagent or the mixture of the reagent and the sample in the reaction cup on the reagent disk. The pipetting mechanism has a pipetting head, which can move up and down repeatedly relative to the reaction disk. Through the coordination of the up and down movement of the pipetting head and the rotation of the reaction disk, the reagents and samples in the reagent disk can be added with samples, reagents, and mixed.

[0027] By adopting the above technical solution, when it is necessary to perform biochemical testing on the sample according to the biochemical testing items preset on the reagent disk, the staff fixes the disk body of the reagent disk to the reaction disk through the mounting part, and the reaction disk can drive the reagent disk to rotate horizontally, so that the sample cup, reagent cup, reaction cup and liquid storage cup can all be moved to the position aligned with the pipetting head in the pipetting mechanism. The pipetting mechanism can then realize the transfer of the sample in the sample cup to the reaction cup, the transfer of the reagent in the reagent cup to the reaction cup, the absorption of the diluent from the liquid storage cup to the reagent cup or reaction cup to redissolve the solid reagent therein, the cleaning of the pipetting head or the suction tip, and the mixing of the sample and reagent transferred to the reaction cup. When the mixing operation is completed, the optical signal detection mechanism performs optical signal analysis and detection on the reagent or the mixture of the reagent and the sample, so that multiple items of the sample can be quickly tested, and the instrument structure is simple.

[0028] Preferably, a constant temperature heating device is provided in the reaction disk, and the constant temperature heating device heats the reaction disk and heats the reagents in the reagent disk and maintains a predetermined temperature through heat conduction.

[0029] By adopting the above technical solution, the constant temperature heating device heats the reaction disk and heats the reagents in the reagent disk and maintains a predetermined temperature through heat conduction, providing a stable temperature environment for biochemical testing, which is conducive to ensuring the stability and reliability of biochemical test results.

[0030] Preferably, the pipetting mechanism includes a pipetting module and a lifting assembly. The pipetting module uses piston-type motion to generate air pressure, which is transmitted to the pipetting head through a pipeline. The pipetting head is configured as a small pipeline. The pipetting head inhales and discharges liquid to achieve liquid movement and distribution. The pipetting head is fixed on the lifting assembly. The pipetting head is located vertically above the reaction disk. As the reaction disk rotates, the lower end of the pipetting head can be aligned with the sample cup, the reagent cup, the reaction cup and the liquid-containing cup of the reagent disk respectively. The lifting assembly is disposed on one side of the reaction disk to drive the pipetting head to move vertically up and down.

[0031] By adopting the above technical solution, as the reaction disk rotates, the lower end of the pipette head can be aligned with the sample cup, reagent cup, reaction cup and liquid cup respectively, so as to complete operations such as adding samples, adding reagents, and mixing. When the reaction disk rotates to the point where the lower end of the pipette head is aligned with the sample cup, the lifting component drives the pipette head to move vertically up and down to aspirate the sample in the sample cup; when the reaction disk rotates to the point where the lower end of the pipette head is aligned with the reagent cup, the lifting component drives the pipette head to move vertically up and down to aspirate the reagent in the reagent cup; when the reaction disk rotates to the point where the lower end of the pipette head is aligned with the reaction cup, the lifting component drives the pipette head to move vertically downward, so that the pipette head can be inserted into the reaction cup to achieve mixing of the liquid in the reaction cup by repeatedly sucking and releasing the liquid; other operations are performed in the same manner as above and are not listed one by one. In short, through the rotation of the reaction disk, the up and down movement of the pipette head, and the aspiration and discharge actions of the pipette head, the operations required for biochemical testing such as adding samples to the reaction cup, adding reagents to the reaction cup, mixing the liquid in the reaction cup, cleaning the pipette head / tip in the cleaning solution, adding diluent to the reagent cup, adding diluent to the reaction cup, and diluting the sample can be achieved.

[0032] Preferably, the pipetting head can be moved vertically downward to be detachably and tightly connected with the pipette head placed in the pipette head position.

[0033] By adopting the above technical solution, operations such as adding samples, adding reagents, and mixing can be performed by replacing the pipette head with a suction tip, which can greatly reduce the difficulty of cleaning the pipette head and thus greatly reduce cross contamination of biochemical reactions.

[0034] Preferably, a punching mechanism is further included, which is used to punch holes in the sealing member on the reagent disk, so that the pipette head or suction tip can add samples or perform mixing operations on the sealed reagent cup, the reaction cup and the liquid containing cup without puncture.

[0035] By adopting the above technical solution, the punching mechanism punches holes in the seal so that the pipette head can smoothly add samples or mix them in the reagent cup, reaction cup and liquid cup. Otherwise, before the test, the sealing layer needs to be torn open manually or the pipette head needs to have a puncture function.

[0036] Preferably, the optical signal detection mechanism includes a support seat, a light source, a lens, a filter and an optical signal receiving plate. The support seat is located on one side of the outer periphery of the reaction disk, the light source, the lens and the filter are installed inside the support seat, and the optical signal receiving plate is installed inside the reaction disk. The side wall of the reaction disk is provided with a light-transmitting hole on the light detection path. When the reagent disk is detachably connected to the reaction disk and optical signal detection is performed on the reagent or the mixture of sample and reagent in the reaction cup of the reagent disk, the light source, the lens, the filter, the reaction cup and the optical signal receiving plate are located on the same straight line, and the light emitted by the light source passes through the lens, the filter and the reaction cup in turn and hits the optical signal receiving plate.

[0037] By adopting the above technical solution, the support seat supports the light source, lens and filter. When the reaction cup needs to be detected, the reaction disk transfers the reaction cup to be detected into the optical path of the optical signal detection mechanism.

[0038] Preferably, the optical signal detection mechanism can also be designed using the post-spectroscopy principle. When this scheme is used to perform optical signal detection on the reaction cup, the light source, reaction cup, filter or grating, and optical signal receiving plate are located on the same straight line. The light emitted by the light source passes through the reaction cup, filter or grating in turn, and finally illuminates the optical signal receiving plate, thereby realizing the detection of the optical signal.

[0039] Preferably, the pipetting head of the pipetting mechanism is equipped with an automatic cleaning device, and the automatic cleaning device automatically cleans the pipetting head.

[0040] By adopting the above technical solution, the automatic cleaning device automatically cleans the pipetting head. Without making major adjustments to the basic structure of the instrument, the liquid cup in the reagent tray used for containing the cleaning liquid can be omitted, thereby reducing the complexity of the reagent tray and the volume of the reagent tray, which is conducive to further improving the convenience of the system. When the automatic cleaning technology for the pipetting head is adopted, the suction tip is not used to replace the pipetting head to perform sample addition, reagent addition, and mixing operations.

[0041] In summary, this application includes at least one of the following beneficial technical effects:

[0042] 1. By integrating the sample cup, reagent cup, reaction cup, and liquid container on a single tray, the resource elements required for multiple biochemical testing items can be integrated on a single tray. The rotation of the tray allows each resource element to be conveniently controlled, thereby greatly reducing the difficulty of providing elements for multiple biochemical testing items, improving the convenience of reagent provision, and greatly reducing dependence on biochemical testing instruments, simplifying the instrument structure.

[0043] 2. The biochemical analyzer completes all the actions required for biochemical testing, such as adding samples, adding reagents, mixing, cleaning the pipette head / tip, and adding diluent, simply by rotating the reaction disk and moving the pipette head of the pipetting mechanism up and down. This greatly simplifies the instrument structure, reduces the instrument cost, reduces the instrument size, makes the instrument easy to operate, and facilitates the product's application in scenarios requiring on-site rapid testing.

[0044] 3. The first and second reagents are solid reagents and are reconstituted with a diluent before use on the reagent disc. This can reduce the temperature requirements for storage and transportation of the reagent disc and further improve the applicability of the reagent disc.

[0045] 4. By installing a suction tip on the pipetting head instead of the pipetting head to perform various liquid-contact operations, the difficulty of cleaning the pipetting head can be greatly reduced, reducing the risk of cross-infection. By installing an automatic cleaning device on the pipetting head to clean the pipetting head, the liquid cup in the reagent tray used to hold the cleaning solution can be eliminated without making major adjustments to the basic structure of the instrument. This can reduce the complexity and volume of the reagent tray, which is conducive to further improving the convenience of the biochemical analyzer. BRIEF DESCRIPTION OF THE DRAWINGS

[0046] FIG1 is a schematic structural diagram of the reagent disc after removing the sealing member in Example 1 of the present application.

[0047] FIG2 is a schematic structural diagram of the reagent disk retaining the sealing member in Example 1 of the present application.

[0048] FIG3 is a schematic structural diagram of the biochemical analyzer in Example 2 of the present application.

[0049] FIG4 is a schematic structural diagram of the biochemical analyzer in Example 2 of the present application from another perspective.

[0050] FIG5 is a cross-sectional view of the optical signal detection mechanism of the biochemical analyzer in Example 2 of the present application.

[0051] FIG6 is an enlarged view of portion A in FIG5.

[0052] Explanation of the accompanying symbols: 1. Tray body; 2. Sample cup; 3. Reagent cup; 4. Reaction cup; 5. Seal; 6. Liquid containing cup; 7. Tip position; 8. Tip; 9. Connection structure; 10. Reaction tray; 11. Pipetting mechanism; 111. Pipetting module; 112. Lifting assembly; 1121. Support column; 1122. Mounting seat; 1123. Driving member; 11231. Driving motor; 11232. Driving screw; 1124. Stabilizing block; 113. Pipetting head; 12. Optical signal detection mechanism; 121. Support seat; 122. Light source; 123. Lens; 124. Filter; 125. Optical signal receiving board; 13. Limit block. DETAILED DESCRIPTION

[0053] The present application is further described in detail below with reference to Figures 1-6.

[0054] Example 1:

[0055] Implementation 1 of the present application discloses a reagent disc, referring to FIG1 , comprising a disc body 1, the disc body 1 being arranged in a disc shape, a circular working area being arranged at the edge of the disc body 1, and at least one sample cup 2, a plurality of reagent cups 3 and a plurality of reaction cups 4 being arranged along the circular track of the circular working area for performing multi-item detection of biochemical analysis.

[0056] 1 , each reaction cup 4 is pre-loaded with the first reagent required for all biochemical test items preset on the reagent tray. The first reagent is a liquid reagent or a solid reagent. The reaction cup 4 is a transparent cup. In this embodiment, the reaction cup 4 is designed as a colorimetric cup commonly used in biochemical analyzers to facilitate optical signal detection of the reagent or the mixture of the reagent and the sample in the reaction cup 4.

[0057] 1 , each reagent cup 3 is pre-installed with a second reagent required for all biochemical test items preset on the reagent disk. The second reagent is a liquid reagent or a solid reagent.

[0058] Referring to Figure 1 , the disk body 1 is further provided with a liquid cup 6 along its circular trajectory in the annular working area. The liquid cup 6 is pre-filled with a cleaning solution and / or a diluent. Referring to Figure 3 , the cleaning solution is used to clean the pipette head 113 or the pipette tip 8, and the diluent is used to dilute the sample or to reconstitute the solid reagents in the reaction cup 4 or the reagent cup 3. Specifically, when the first and second reagents are solid reagents, the diluent in the liquid cup 6 is used to reconstitute the solid reagents to dissolve the solid reagents into liquid reagents.

[0059] 1 and 2 , the tray 1 is further provided with a sealing member 5 , which is used to seal the reagent cup 3 , reaction cup 4 , and liquid cup 6 and is sealed therewith. The sealing member 5 is tightly coupled to the openings of the reagent cup 3 , reaction cup 4 , and liquid cup 6 by heat pressing, facilitating pre-infusion of the reagents in the reagent cup 3 and reaction cup 4 and the cleaning solution and / or diluent in the liquid cup 6 , and facilitating storage and transportation of the pre-infused reagents. The sealing member 5 is any one of an aluminum foil sealing film, a silicone sealing film, a plastic sealing film, and a rubber sealing film.

[0060] Referring to Figure 1 , the disk 1 is provided with a tip position 7 along its circular trajectory within the annular working area. Referring to Figure 3 , tip position 7 is used to accommodate a tip 8, facilitating its attachment to a pipette head 113 used for reagent transfer. It should be noted that tip 8 is mounted on pipette head 113 to replace it in liquid aspiration and dispensing operations. Because tip 8 is replaceable, cleaning of pipette head 113 is avoided, significantly reducing the risk of cross-infection.

[0061] 1 , the disc body 1 is provided with a connection structure 9 for detachable connection with a biochemical analyzer. The disc body 1 is mounted on the biochemical analyzer via the connection structure 9 to perform biochemical test.

[0062] 1 , the reagent tray is integrally formed, with the tray body 1 and various cup-shaped components being integrally injection-molded to improve production efficiency and consistency of the reagent tray, thereby reducing costs and achieving disposable use.

[0063] The implementation principle of a reagent tray in Example 1 of the present application is as follows: the sample cup 2, the reagent cup 3, the reaction cup 4 and the liquid cup 6 are integrated on a tray body 1, so that the samples, reaction cup 4, the first reagent, the second reagent, the cleaning liquid and other elements required for biochemical testing of various projects are integrated on a reagent tray, and the reagent tray can be flexibly controlled and used by rotating it, so that the elements of biochemical testing are concentrated and integrated, which greatly reduces the dependence on the mechanical structure of the instrument, simplifies the instrument structure, and can make the instrument miniaturized and simple to operate.

[0064] Example 2:

[0065] Example 2 of the present application discloses a biochemical analyzer, which, with reference to FIG3 , includes a reaction disk 10, a pipetting mechanism 11, and an optical signal detection mechanism 12. The reaction disk 10 is arranged to rotate horizontally. The reaction disk 10 is provided with a mounting portion for detachably connecting to the disk body 1 of the reagent disk. The connecting structure 9 of the disk body 1 is snap-fitted into the mounting portion of the reaction disk 10 to achieve installation of the reagent disk on the reaction disk 10. At the same time, the rotation of the reaction disk 10 can drive the reagent disk to rotate synchronously. The optical signal detection mechanism 12 is used to perform optical signal detection on the reagent or the mixture of the reagent and the sample in the reaction cup 4 on the reagent disk. The pipetting mechanism 11 has a pipetting head 113. The pipetting head 113 can repeatedly move up and down relative to the reaction disk 10. The reagent and sample in the reagent disk can be transferred and mixed by cooperating with the rotation of the pipetting head 113 and the reaction disk 10.

[0066] The pipetting mechanism 11 transfers the sample in the sample cup 2 in the reagent disk to the reaction cup 4, transfers the reagent in the reagent cup 3 to the reaction cup 4, draws the diluent from the liquid containing cup 6 to the reagent cup 3 or the reaction cup 4 to redissolve the solid reagent therein, cleans the pipetting head 113 or the suction tip 8 of the pipetting mechanism 11, and mixes the sample and reagent transferred to the reaction cup 4, etc. The optical signal detection mechanism 12 is used to analyze and detect the optical signal of the reagent or the mixture of the reagent and the sample in the reaction cup 4.

[0067] 3 and 4 , the pipetting mechanism 11 includes a pipetting module 111 and a lifting assembly 112. The pipetting module 111 uses piston motion to generate air pressure that is transmitted to a pipetting head 113 through a pipe. The pipetting head 113 is configured as a small pipe. The pipetting head 113 inhales and discharges liquid to achieve quantitative movement and distribution of the liquid. The pipetting head 113 is fixed on the lifting assembly 112. The pipetting head 113 is located above the reaction disk 10 and is vertically arranged. As the reaction disk 10 rotates, the lower end of the pipetting head 113 is aligned with the sample cup 2, the reagent cup 3, the reaction cup 4 and the liquid-containing cup 6 respectively. The lifting assembly 112 is arranged on one side of the reaction disk 10 to drive the pipetting head 113 to move vertically up and down.

[0068] 4 , the lifting assembly 112 includes a support column 1121, a mounting seat 1122 and a driving member 1123. The support column 1121 is vertically fixed on one side of the reaction disk 10. The mounting seat 1122 is slidably connected to the side wall of the support column 1121, and the mounting seat 1122 extends toward the direction of the reaction disk 10. The pipetting head 113 is vertically mounted on the side of the mounting seat 1122 facing the reaction disk 10, so that the pipetting head 113 is located above the edge of the reaction disk 10. The driving member 1123 is fixedly mounted on the side wall of the support column 1121 and is used to drive the mounting seat 1122 to move vertically up and down. The driving member 1123 drives the mounting seat 1122 to move vertically up and down to drive the pipetting head 113 to move vertically up and down.

[0069] 4 , the driving member 1123 includes a driving motor 11231 and a driving screw 11232. In this embodiment, the driving motor 11231 is selected as a servo motor that can rotate forward and reverse. The driving motor 11231 is fixedly mounted on the side wall of the support column 1121. The output shaft of the driving motor 11231 is arranged vertically upward. The driving screw 11232 is coaxially fixedly connected to the output shaft of the driving motor 11231, and the driving screw 11232 is threadedly penetrated into the mounting seat 1122. A side wall of the mounting seat 1122 abuts and slides on the side wall of the support column 1121. The output shaft of the driving motor 11231 rotates to drive the driving screw 11232 to rotate. The driving screw 11232 rotates to drive the mounting seat 1122 to move vertically up and down on the side wall of the support column 1121, thereby realizing the vertical up and down movement of the pipetting head 113 vertically mounted on the mounting seat 1122.

[0070] 4 , the lifting assembly 112 further includes a stabilizing block 1124, which is horizontally fixed to the side wall of the support column 1121. A bearing is rotatably provided in the middle of the stabilizing block 1124, and the drive screw 11232 is rotatably provided through the stabilizing block 1124 via the bearing, thereby improving the stability of the drive screw 11232 during rotation. It is worth mentioning that the stabilizing block 1124 is located at the lower end of the drive screw 11232 to limit the vertical downward movement of the mounting seat 1122. Accordingly, a limit block 13 is provided at the upper end of the drive screw 11232, which is horizontally fixed to the side wall of the support column 1121. A bearing is also rotatably provided in the middle of the limit block 13, and the upper end of the drive screw 11232 is rotatably provided through the limit block 13 via the bearing to limit the vertical upward movement of the mounting seat 1122.

[0071] 5 and 6, when it is necessary to perform optical signal detection on the reagent blank, the reagent after sample addition, and the reaction mixture after the second reagent is added, the reaction disk 10 is rotated to the position where the reaction cup 4 is aligned with the optical path of the optical signal detection mechanism 12 to perform analysis and detection operations. Specifically, the optical signal detection mechanism 12 includes a support seat 121, a light source 122, a lens 123, a filter 124 and an optical signal receiving plate 125. The support seat 121 is fixedly arranged on the upper plate surface of the support plate, and the support seat 121 is located on one side of the outer periphery of the reaction disk 10. The light source 122, the lens 123 and the filter 124 are installed inside the support seat 121, and the light source 122, the lens 123 and the filter 124 are located on the same horizontal plane. The optical signal receiving plate 125 is installed on the reaction disk 1 Inside the reaction tray 10, a light-transmitting hole is formed on the side wall facing the support seat 121. When the reaction cup 4 containing the mixed reagent and sample is rotated to a position facing the support seat 121, the light source 122, lens 123, filter 124, reaction cup 4, and optical signal receiving board 125 are aligned. The detection light emitted by the light source 122 passes through the lens 123 and filter 124, enters the mixed reagent and sample in the reaction cup 4, and finally reaches the optical signal receiving board 125 for analysis and detection.

[0072] Referring to Figure 3 , a constant-temperature heating device is installed within the reaction disk 10. This device heats the reaction disk 10 and, through heat conduction, heats the reagents in the reagent disk and maintains them at a predetermined temperature. In addition, the biochemical analysis system includes a perforating mechanism and an automatic cleaning device. The perforating mechanism is used to perforate the sealing member 5, allowing the pipetting head 113 to perform sample addition or mixing operations on the sealed reagent cup 3, reaction cup 4, and liquid container 6 without puncturing. The automatic cleaning device automatically cleans the pipetting head 113. This is a common feature of pipetting equipment and will not be described in detail here.

[0073] The implementation principle of a biochemical analyzer in Example 2 of the present application is as follows: when it is necessary to analyze and test the biochemical items preset on the reagent disk, the biochemical analyzer is first turned on, and the constant temperature heating device in the reaction disk 10 automatically heats and maintains the temperature at 37°C ± 0.2°C, then the reagent disk is installed on the reaction disk 10, the pipette tip 8 is inserted into the pipette tip position 7, the sample to be tested is added to the sample cup 2, the aluminum foil 5 on the reagent disk is manually torn off, and after the detection command is issued to the biochemical analyzer, the reaction disk 10 is rotated to the position where the pipette tip position 7 is directly below the pipetting head 113, and the pipetting head 113 is driven by the lifting component 112 to move vertically downward to tightly cover the pipette tip 8 on the lower end of the pipetting head 113 to complete the automatic installation of the pipette tip 8, and the liquid in the reaction cup 4 and the reagent cup 3 of the reagent disk is added. After the temperature is raised and maintained constant at 37°C ± 0.2°C, the reaction disk 10 rotates, the pipette tip 8 moves up and down, the pipette mechanism 11 sucks and releases the liquid, the reaction disk 10 rotates in coordination with the optical signal detection mechanism 12 to detect the optical signal of the reagent or the mixture of the reagent and the sample in the reaction cup 4, and the pipette tip 8 performs a cleaning operation between two different operation actions. All biochemical test items preset on the reagent disk are completed according to their respective test procedures. The optical signal intensity of each analysis stage of all preset biochemical test items is obtained, and the absorbance value or absorbance value change of each biochemical test item is calculated. After comparison and calculation with the calibration curve of all biochemical test items preset on the reaction disk, the test results of all biochemical test items preset on the reagent disk in the sample can be calculated.

[0074] The above are all preferred embodiments of the present application, and are not intended to limit the scope of protection of the present application. Therefore, any equivalent changes made based on the structure, shape, and principle of the present application should be included in the scope of protection of the present application.

Claims

1. A reagent disc, characterized in that: The invention comprises a disk body (1), wherein the disk body (1) is provided with a circular working area, and at least one sample cup (2), a plurality of reagent cups (3) and a plurality of reaction cups (4) are arranged on the circular working area along its circular track.

2. A reagent disc according to claim 1, characterized in that: The tray body (1) is further provided with a sealing member (5), and the sealing member (5) is used to seal the reagent cup (3) and the reaction cup (4) and is sealedly connected to the reagent cup (3) and the reaction cup (4).

3. A reagent disc according to claim 2, characterized in that: Each of the reaction cups (4) is pre-installed with a first reagent required for all biochemical detection items preset in the reagent disk, the first reagent is a liquid reagent or a solid reagent, and the reaction cup (4) is a transparent material cup.

4. A reagent disc according to claim 2, characterized in that: Each of the reagent cups (3) is pre-installed with a second reagent required for all biochemical detection items preset on the reagent disk, and the second reagent is a liquid reagent or a solid reagent.

5. A reagent disc according to claim 2, characterized in that: The disk body (1) is also provided with a liquid containing cup (6) along its circular track in the circular working area, the liquid containing cup (6) is pre-filled with cleaning liquid and / or diluent, and the liquid containing cup (6) is sealed by the sealing member (5).

6. A reagent disc according to claim 1, characterized in that: The disk body (1) is also provided with a suction head position (7) along its circular track in the circular working area, and the suction head position (7) is used to place a suction head (8).

7. A reagent disc according to claim 2, characterized in that: The sealing member (5) is any one of an aluminum foil sealing film, a silicone sealing film, a plastic sealing film and a rubber sealing film.

8. A reagent disc according to claim 1, characterized in that: The disk body (1) is provided with a connection structure (9) for detachably connecting with a biochemical analyzer.

9. A reagent disc according to claim 1, characterized in that: The reagent disc is integrally formed.

10. A biochemical analyzer, characterized in that: The invention comprises a reaction disk (10), a pipetting mechanism (11) and an optical signal detection mechanism (12); the reaction disk (10) is arranged to rotate horizontally; the reaction disk (10) is provided with a mounting portion for detachably connecting with a disk body (1) of a reagent disk according to any one of claims 1 to 9; the optical signal detection mechanism (12) is used to detect an optical signal of a reagent or a mixture of a reagent and a sample in a reaction cup (4) on the reagent disk; the pipetting mechanism (11) has a pipetting head (113); the pipetting head (113) can repeatedly move up and down relative to the reaction disk (10); and the operation of adding samples, adding reagents and mixing the reagents and samples in the reagent disk can be realized by the cooperation of the up and down movement of the pipetting head (113) and the rotation of the reaction disk (10).

11. A biochemical analyzer according to claim 10, characterized in that: A constant temperature heating device is arranged inside the reaction disk (10), and the constant temperature heating device heats the reaction disk (10) and heats the reagent in the reagent disk through heat conduction so that the reagent is heated and maintained at a predetermined temperature.

12. A biochemical analyzer according to claim 10, characterized in that: The liquid transfer mechanism (11) comprises a liquid transfer module (111) and a lifting assembly (112). The liquid transfer module (111) uses a piston-type motion to generate air pressure which is transmitted to a liquid transfer head (113) through a pipeline. The liquid transfer head (113) is configured as a small pipeline. The liquid transfer head (113) inhales and discharges liquid to achieve liquid movement and distribution. The liquid transfer head (113) is fixed on the lifting assembly (112). The liquid transfer head (113) is located above the reaction disk (10) and is vertically arranged. As the reaction disk (10) rotates, the lower end of the liquid transfer head (113) can be aligned with the sample cup (2), the reagent cup (3), the reaction cup (4) and the liquid containing cup (6) of the reagent disk respectively. The lifting assembly (112) is arranged on one side of the reaction disk (10) and is used to drive the liquid transfer head (113) to move vertically up and down.

13. A biochemical analyzer according to claim 10, characterized in that: The pipetting head (113) can move vertically downward to be detachably and tightly connected with the pipette head (8) placed in the pipette head position (7).

14. A biochemical analyzer according to claim 10, characterized in that: The optical signal detection mechanism (12) comprises a support seat (121), a light source (122), a lens (123), a filter (124) and an optical signal receiving plate (125); the support seat (121) is located on one side of the outer periphery of the reaction disk (10); the light source (122), the lens (123) and the filter (124) are installed inside the support seat (121); the optical signal receiving plate (125) is installed inside the reaction disk (10); and a side wall of the reaction disk (10) is provided on a path for optical detection. There is a light-transmitting hole. When the reagent disk is detachably connected to the reaction disk (10) and optical signal detection is performed on the reagent or the mixture of the sample and the reagent in the reaction cup (4) of the reagent disk, the light source (122), the lens (123), the filter (124), the reaction cup (4) and the optical signal receiving plate (125) are located on the same straight line. The light emitted by the light source (122) passes through the lens (123), the filter (124) and the reaction cup (4) in sequence and then hits the optical signal receiving plate (125).

15. A biochemical analyzer according to claim 10, characterized in that: The liquid transfer head (113) of the liquid transfer mechanism (11) is equipped with an automatic cleaning device, and the automatic cleaning device automatically cleans the liquid transfer head (113).

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