Enrichment device for sample concentration

Abstract

An enrichment device for sample concentration, comprising a support (100), a concentration container (200) and a magnetic component (300), wherein the support (100) is provided with an accommodating cavity (110); the concentration container (200) is mounted in the accommodating cavity (110), and the concentration container (200) is used for containing an eluent; and the magnetic component (300) is arranged on the support (100) and located below the accommodating cavity (110). When a magnetic bar assembly having magnetic beads is inserted into the concentration container (200), the magnetic beads can be attracted by the magnetic component (300) to separate from the magnetic bar assembly and then fall into the eluent.

Description

Concentrated sample enrichment device

[0001] This application claims priority to the Chinese patent application filed with the China Patent Office on November 24, 2023, with application number 202311583697.5. The entire contents of the above application are incorporated by reference into this application. Technical Field

[0002] The present application relates to the field of biomedical engineering technology, for example, to a concentrated sample enrichment device. Background Art

[0003] Currently available pathogen nucleic acid enrichment and sampling instruments are based on traditional techniques such as polyethylene glycol precipitation, aluminum salt coagulation precipitation, and centrifugal ultrafiltration. These instruments suffer from cumbersome operation, low automation, the need for on-site sampling by specialized technicians, complex system integration, and portability. Furthermore, sewage composition is complex, and precipitation methods present numerous interfering factors that affect detection accuracy. Conventional sewage has low viral content and requires a high concentration rate, which can easily lead to significant errors due to different operators. Therefore, a new nucleic acid extraction technology based on magnetic beads and magnetic rods has emerged.

[0004] During the nucleic acid extraction process based on the magnetic bead and magnetic rod method, magnetic beads are needed to enrich the pathogens because the concentration of pathogens in sewage is generally low.

[0005] Summary of the Invention

[0006] The present application discloses a concentrated sample enrichment device, comprising: a bracket, on which a accommodating cavity is provided; a concentration container, which is installed in the accommodating cavity and is used to hold an eluent; a magnetic component, which is provided on the bracket and located below the accommodating cavity; wherein, after a magnetic rod assembly with magnetic beads is inserted into the concentrating container, the magnetic beads can detach from the magnetic rod assembly under the attraction of the magnetic component and fall into the eluent.

[0007] Additional aspects and advantages of the present application will be given in part in the description below, and in part will become obvious from the description below, or will be learned through practice of the present application. BRIEF DESCRIPTION OF THE DRAWINGS

[0008] FIG1 is a schematic structural diagram of a sample concentration and enrichment device according to Example 1 of the present application;

[0009] FIG2 is a schematic structural diagram of a sample concentration and enrichment device according to a second embodiment of the present application.

[0010] Figure numerals: 100, bracket; 110, accommodating chamber; 111, straight hole section; 112, tapered hole section; 120, mounting groove; 121, support groove; 200, concentration container; 210, straight pipe section; 220, tapered pipe section; 300, magnetic component; 400, protective cover; 410, support ear; 500, connecting bolt. DETAILED DESCRIPTION

[0011] In the description of the present application, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", "axial", "radial", "circumferential" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present application and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present application.

[0012] In addition, features defined as "first" or "second" may explicitly or implicitly include one or more of the features, and are used to distinguish and describe features, without distinction of order or importance. In the description of this application, unless otherwise specified, "plurality" means two or more.

[0013] In the description of this application, it should be noted that, unless otherwise expressly specified or limited, the terms "mounted," "connected," and "connected" should be understood in a broad sense. For example, they can refer to fixed connections, detachable connections, or integral connections; mechanical connections or electrical connections; direct connections or indirect connections through an intermediate medium; and internal connections between two components. Those skilled in the art will understand the specific meanings of the above terms in this application based on the specific circumstances.

[0014] During the nucleic acid extraction process using magnetic beads and magnetic rods, due to the generally low concentration of pathogens in wastewater, magnetic beads are required for pathogen enrichment. Furthermore, since the volume of wastewater to be treated typically ranges from 500-1000 mL, a relatively large amount of magnetic beads is used. After the magnetic beads capture the pathogens in the wastewater, the magnetic rod and magnetic rod cover will magnetically attract the magnetic beads from the wastewater. Due to the large volume of wastewater being treated and the large number of magnetic beads used, a thicker magnetic rod and magnetic rod cover are required. These rods and magnetic rod covers must be repeatedly attracted and eluted to quickly and completely capture the magnetic beads from the wastewater. After the magnetic rod and magnetic rod cover have attracted the magnetic beads, the beads outside the magnetic rod cover need to be eluted into the eluent. Because the magnetic rod and magnetic rod cover used are thicker, and the eluent needs to cover the magnetic bead adsorption area on the magnetic rod cover, a larger volume of eluent is required to completely elute the magnetic beads outside the magnetic rod cover, which directly affects the pathogen enrichment concentration.

[0015] To address the above situation, an embodiment of the present application provides a concentrated sample enrichment device.

[0016] The structure of the sample concentration and enrichment device according to an embodiment of the present application will be described below with reference to FIG. 1 and FIG. 2 .

[0017] An embodiment of the present application discloses a concentrated sample enrichment device, as shown in Figures 1-2, the concentrated sample enrichment device includes a bracket 100, a concentration container 200 and a magnetic component 300. The bracket 100 is provided with a accommodating chamber 110, the concentration container 200 is installed in the accommodating chamber 110, and the concentration container 200 is used to hold an eluent. The magnetic component 300 is provided on the bracket 100 and is located below the accommodating chamber 110. After the magnetic rod assembly with magnetic beads is inserted into the concentration container 200, the magnetic beads can be separated from the magnetic rod assembly and fall into the eluent under the attraction of the magnetic component 300. It can be understood that in the actual working process, an appropriate amount of eluent is injected into the concentration container 200, and then the magnetic rod assembly with magnetic beads is inserted into the concentration container 200. Through the magnetic attraction of the magnetic component 300 on the magnetic beads, the magnetic beads are quickly and fully transferred from the magnetic rod sleeve to the eluent. There is no need for the magnetic rod sleeve to oscillate and elute in the eluent to achieve complete transfer, which avoids the adsorption and residue of magnetic beads on the outer surface of the magnetic rod assembly, shortening the time required for enrichment and concentration. Since the concentrated sample enrichment device uses the magnetic attraction of the magnetic component 300 on the magnetic beads to adsorb the magnetic beads into the eluent, there is no need for the eluent to submerge the magnetic bead attachment area, which effectively reduces the amount of eluent used, thereby doubling the pathogen concentration ratio of the sewage.

[0018] In some embodiments, as shown in Figures 1 and 2, the lower surface of the bracket 100 is provided with a mounting groove 120, and the magnetic component 300 is installed in the mounting groove 120. It is understood that, compared to installing the magnetic component 300 on the lower surface of the bracket 100, the example of providing the mounting groove 120 on the lower surface of the bracket 100 and then installing the magnetic component 300 in the mounting groove 120 can shorten the distance between the magnetic component 300 and the bottom of the concentration container 200, ensuring that the magnetic component 300 can stably adsorb the magnetic beads from the magnetic rod assembly, further preventing the magnetic beads from being adsorbed and remaining on the outer surface of the magnetic rod assembly.

[0019] In some embodiments, as shown in Figures 1 and 2, the sample concentration and enrichment device further includes a protective cover 400, which is sleeved over the magnetic component 300 and installed in the installation slot 120. It is understood that the protective cover 400 can wrap the magnetic component 300 to prevent the magnetic component 300 from adsorbing contaminants and ensure the cleanliness of the magnetic component 300.

[0020] In some embodiments, an adhesive layer is provided on the inner wall of the protective cover 400, and the magnetic component 300 is bonded to the protective cover 400 via the adhesive layer. It is understood that bonding the magnetic component 300 to the protective cover 400 by bonding can ensure the stability of the connection between the magnetic component 300 and the protective cover 400 and prevent the magnetic component 300 from falling out of the protective cover 400.

[0021] In some embodiments, as shown in FIG1 , a first mounting hole is provided on the top wall of the mounting groove 120, and a second mounting hole is provided on the protective cover 400 corresponding to the first mounting hole. The first mounting hole is a threaded hole, and the second mounting hole is a through hole; a connecting bolt 500 passes through the second mounting hole and cooperates with the first mounting hole to secure the protective cover 400 to the bracket 100. It is understood that during the actual installation process, the magnetic component 300 is first bonded to the protective cover 400 via the adhesive layer, and then the protective cover 400 is installed in the mounting groove 120 so that the first mounting hole and the second mounting hole are aligned. The connecting bolt 500 is then inserted to lock the protective cover 400. This installation method is convenient to operate, facilitates the installation and removal of the protective cover 400, and can improve the connection stability between the protective cover 400 and the bracket 100, thereby ensuring that the magnetic component 300 can be stably installed on the bracket 100.

[0022] In some embodiments, there are multiple first mounting holes and multiple second mounting holes, and the multiple second mounting holes are spaced apart around the magnetic component 300. It is understood that the multiple connecting bolts 500 can improve the connection stability between the protective cover 400 and the bracket 100, thereby ensuring that the magnetic component 300 can be stably mounted on the bracket 100.

[0023] In some embodiments, as shown in FIG2 , the sidewall of the mounting groove 120 has a support groove 121 extending through the bracket 100, and the outer peripheral wall of the protective cover 400 is provided with a support ear 410 that fits within the support groove 121. It is understood that during the actual installation process, the magnetic component 300 is first bonded to the protective cover 400 via the adhesive layer, and then the support ear 410 is aligned with the support groove 121, and then the protective cover 400 is pushed into the mounting groove 120. This installation method is convenient and does not require the use of connectors, making it easy to install and remove the protective cover 400.

[0024] In some embodiments, the support grooves 121 are provided in pairs, and the paired support grooves 121 are respectively located on two opposite side walls of the mounting groove 120. It is understood that the cooperation between the multiple support ears 410 and the support grooves 121 can improve the connection stability between the protective cover 400 and the bracket 100, thereby ensuring that the magnetic component 300 can be stably installed on the bracket 100.

[0025] 1-2 , the concentrating container 200 includes a straight pipe section 210 and a tapered pipe section 220 connected to the bottom of the straight pipe section 210. It is understood that the tapered pipe section 220 can facilitate the insertion of the concentrating container 200 into the accommodating chamber 110.

[0026] In some embodiments, as shown in Figures 1-2, the accommodating chamber 110 includes a straight bore section 111 and a tapered bore section 112 connected to the bottom of the straight bore section 111. The straight bore section 111 is used to accommodate the straight pipe section 210, and the tapered bore section 112 is used to accommodate the tapered pipe section 220. It will be understood that the straight bore section 111 is used to accommodate the straight pipe section 210, and the tapered bore section 112 is used to accommodate the tapered pipe section 220, which can ensure the stability of the concentrating container 200 after being inserted into the accommodating chamber 110.

[0027] Example 1:

[0028] As shown in Figure 1, the concentrated sample enrichment device includes a bracket 100, a concentration container 200, a magnetic component 300, and a protective cover 400. The concentration container 200 includes a straight tube section 210 and a tapered tube section 220 connected to the bottom of the straight tube section 210. The bracket 100 is provided with a accommodating chamber 110, which includes a straight hole section 111 and a tapered hole section 112 connected to the bottom of the straight hole section 111. The straight hole section 111 is used to accommodate the straight tube section 210, and the tapered hole section 112 is used to accommodate the tapered tube section 220. The lower surface of the bracket 100 is provided with a mounting groove 120. The top wall of the mounting groove 120 is provided with a first mounting hole, which is a threaded hole. The concentration container 200 is installed in the accommodating chamber 110, and the concentration container 200 is used to hold the eluent. The protective cover 400 is sleeved on the magnetic component 300 and installed in the mounting groove 120. The protective cover 400 is provided with a second mounting hole corresponding to the first mounting hole. The second mounting hole is a through hole, and the connecting bolt 500 passes through the second mounting hole and cooperates with the first mounting hole to fix the protective cover 400 to the bracket 100.

[0029] Example 2:

[0030] As shown in Figure 2, the concentrated sample enrichment device includes a bracket 100, a concentration container 200, a magnetic component 300, and a protective cover 400. The concentration container 200 includes a straight tube section 210 and a tapered tube section 220 connected to the bottom of the straight tube section 210. The bracket 100 is provided with a accommodating chamber 110, which includes a straight hole section 111 and a tapered hole section 112 connected to the bottom of the straight hole section 111. The straight hole section 111 is used to accommodate the straight tube section 210, and the tapered hole section 112 is used to accommodate the tapered tube section 220. The lower surface of the bracket 100 is provided with a mounting groove 120, and the side wall of the mounting groove 120 has a support groove 121 provided through the bracket 100. The concentration container 200 is installed in the accommodating chamber 110, and the concentration container 200 is used to hold the eluent. The protective cover 400 is sleeved on the magnetic component 300 and installed in the mounting groove 120. A support ear 410 is provided on the outer peripheral wall of the protective cover 400 and is engaged with the support groove 121 .

[0031] The advantages of the sample concentration and enrichment devices of Example 1 and Example 2 are as follows:

[0032] First: save time and shorten the time for pathogen sample enrichment;

[0033] Second: Improving the concentration ratio can reduce the amount of concentrated eluent used, thereby increasing the concentration ratio before and after pathogen enrichment;

[0034] Third: The transfer of magnetic beads can be more thorough, avoiding the large amount of magnetic beads remaining on the magnetic rod cover due to adsorption during the elution process, thereby improving the elution efficiency.

[0035] Fourth: High integration performance, simple structure, easy installation and use.

[0036] Throughout this specification, references to "some embodiments," "other embodiments," and the like indicate that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the present application. In this specification, schematic representations of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

Claims

1. A concentrated sample enrichment device, comprising: a bracket (100) provided with a receiving cavity (110) thereon; a concentration container (200) installed in the receiving cavity (110) and used for containing an eluent; a magnetic component (300) provided on the bracket (100) and located below the receiving cavity (110); wherein, after a magnetic rod assembly with magnetic beads is inserted into the concentration container (200), the magnetic beads can fall off the magnetic rod assembly under the attraction of the magnetic component (300) and into the eluent.

2. The concentrated sample enrichment device according to claim 1, wherein, an installation groove (120) is provided on the lower surface of the bracket (100), and the magnetic component (300) is installed in the installation groove (120).

3. The concentrated sample enrichment device according to claim 2, further comprising a protective sleeve (400) sleeved on the magnetic component (300) and installed in the installation groove (120).

4. The concentrated sample enrichment device according to claim 3, wherein, an adhesive layer is provided on the inner peripheral wall of the protective sleeve (400), and the magnetic component (300) is adhered to the inside of the protective sleeve (400) through the adhesive layer.

5. The concentrated sample enrichment device according to claim 3, wherein, a first installation hole is provided on the top wall of the installation groove (120), and a second installation hole corresponding to the first installation hole is provided on the protective sleeve (400); wherein: the first installation hole is a threaded hole, and the second installation hole is a through hole; a connecting bolt (500) passes through the second installation hole and cooperates with the first installation hole to fix the protective sleeve (400) to the bracket (100).

6. The concentrated sample enrichment device according to claim 5, wherein, both the first installation hole and the second installation hole are multiple, and the multiple second installation holes are arranged at intervals around the magnetic component (300).

7. The concentrated sample enrichment device according to claim 3, wherein, a support groove (121) penetrating through the bracket (100) is provided on the side wall of the installation groove (120), and a support ear (410) fitted in the support groove (121) is provided on the outer peripheral wall of the protective sleeve (400).

8. The concentrated sample enrichment device according to claim 7, wherein, the support grooves (121) are arranged in pairs, and the paired support grooves (121) are respectively located on two opposite side walls of the installation groove (120).

9. The concentrated sample enrichment device according to any one of claims 1-8, wherein, the concentration container (200) includes a straight pipe section (210) and a tapered pipe section (220) connected to the bottom of the straight pipe section (210).

10. The concentrated sample enrichment device according to claim 9, wherein, The accommodation cavity (110) includes a straight hole section (111) and a tapered hole section (112) connected to the bottom of the straight hole section (111). The straight hole section (111) is used to accommodate the straight pipe section (210), and the tapered hole section (112) is used to accommodate the tapered pipe section (220).

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