Fluid transport system, fluid transport method, and biochemical substance analysis device

Abstract

A fluid transport system, a fluid transport method, and a biochemical substance analysis device. The fluid transport system comprises a fluid storage module, a fluid use module, a fluid distribution module, and a fluid driving module; the fluid storage module is used for storing fluid; the fluid use module comprises a plurality of independent flow channels, wherein each flow channel has one end communicated with the fluid storage module by means of the fluid distribution module and the other end communicated with the fluid driving module; the fluid distribution module is used for selecting the transport direction of the fluid; and the fluid driving module is used for generating a driving force, so that the fluid located in the fluid storage module is independently loaded into each flow channel by means of the fluid distribution module. The fluid transport system of the present application has a simple structure and low cost, can realize the function of independently loading a sample by means of the flow channels, involves simple operations, and can realize automated loading.

Description

Fluid transport system, fluid transport method and biochemical substance analysis equipment Technical Field

[0001] The present application relates to the field of fluid control technology, and in particular to a fluid transport system, a fluid transport method, and a biochemical substance analysis device. Background Art

[0002] In fields such as biology, chemistry, and medicine, common instruments, such as gene sequencers, are often designed based on the core principles of biological or chemical reactions. Substances involved in biological or chemical reactions (such as reagents or biological samples) are typically liquid or gaseous in physics and are collectively referred to as fluids. To carry out the biological or chemical reactions required by the instrument, some container is typically required to serve as a reaction reservoir for these biochemical substances (such as a sequencing slide).

[0003] Currently, in order to increase sequencing throughput, sequencing slides can usually be designed into two or more channels. However, traditional sequencing chips are difficult to achieve independent fluid loading in each channel, and a large number of auxiliary equipment need to be introduced, making the overall sequencing system complex in structure, high in cost, complicated in operation, and low in loading efficiency.

[0004] Summary of the Invention

[0005] In order to solve the above deficiencies of the prior art, it is necessary to provide a fluid transportation system and a fluid transportation method.

[0006] In addition, the embodiments of the present application also provide a biochemical substance analysis device using the fluid transport system.

[0007] In the first aspect, an embodiment of the present application provides a fluid transport system, comprising: a fluid storage module, a fluid usage module, a fluid distribution module and a fluid driving module, wherein the fluid storage module is used to store fluid; the fluid usage module includes multiple independent flow channels, and the flow channels are used to complete biochemical reactions; wherein the liquid inlet end of each of the flow channels is connected to the fluid storage module through the fluid distribution module, and the liquid outlet end of each of the flow channels is connected to the fluid driving module; the fluid distribution module is used to select the transport direction of the fluid; and the fluid driving module is used to generate a driving force so that the fluid located in the fluid storage module is independently loaded into each of the flow channels through the fluid distribution module.

[0008] In some possible embodiments, the fluid storage module includes a sample storage unit and a reagent storage unit, and the fluid distribution module includes a first fluid distribution unit and a second fluid distribution unit that are interconnected. The sample storage unit is connected to the flow channel through the first fluid distribution unit, and the reagent storage unit is connected to the flow channel through the second fluid distribution unit and the first fluid distribution unit. The first fluid distribution unit and the second fluid distribution unit are both used to control the opening or closing of the fluid pipeline according to a preset timing to select the transport direction of the fluid.

[0009] In some possible embodiments, the sample storage unit includes multiple sample containers, each of the flow channels is connected to the corresponding sample container through the first fluid distribution unit, and the driving force is used to enable the biological samples located in the sample containers to be independently loaded into the corresponding flow channels through the first fluid distribution unit.

[0010] In some possible embodiments, the second fluid dispensing unit is further connected to a designated sample container, and the driving force is further used to load the biological sample in the designated sample container into each of the flow channels sequentially through the second fluid dispensing unit and the first fluid dispensing unit.

[0011] In some possible embodiments, the first fluid dispensing unit is also connected to the fluid driving module, and the driving force is also used to load the reagent located in the reagent storage unit into the fluid driving module via the second fluid dispensing unit and the first fluid dispensing unit in sequence.

[0012] In some possible embodiments, the first fluid dispensing unit includes a stacked first stator and a first rotor, the first stator is provided with a first interface communicating with each of the flow channels, a second interface communicating with the driving module, a third interface communicating with the sample storage unit, and a fourth interface communicating with the second fluid dispensing unit, and the first rotor is provided with a plurality of first communication ports, a second communication port, and a third communication port;

[0013] In which, the first rotor is used to change the relative position of the first rotor and the first stator during the rotation process, so that the first interface is connected to the third interface through the first connecting port; or, the fourth interface is connected to all the first interfaces through the second connecting port; or, the second interface is connected to the fourth interface through the third connecting port.

[0014] In some possible embodiments, the second fluid distribution unit includes a second stator and a second rotor, the second stator is provided with a main interface connected to the fourth interface, and a branch interface connected to the reagent storage unit, the second rotor is provided with a fourth communication port for connecting the main interface and the branch interface, and the second rotor is used to change the relative position of the second rotor and the second stator during rotation so that the main interface is connected to the corresponding branch interface through the fourth communication port.

[0015] In some possible embodiments, the fluid driving module includes a plurality of driving components and a reversing component provided on each of the driving components, each of the reversing components is used to communicate with one of the flow channels, and the driving components are used to provide the driving force.

[0016] In some possible embodiments, the fluid transport system further includes an automatic cleaning module, and the automatic cleaning module is used to clean the fluid transport system.

[0017] In some possible embodiments, the fluid storage module includes a reagent storage unit, the reagent storage unit includes a reagent needle, the automatic cleaning module is connected to the outer wall of the reagent needle, and the automatic cleaning module is also used to provide a driving force to transport the cleaning fluid located in the automatic cleaning module to the outer wall of the reagent needle to clean the outer wall of the reagent needle.

[0018] In some possible embodiments, the fluid storage module includes a reagent storage unit, the reagent storage unit includes a reagent needle, the automatic cleaning module is connected to the reagent needle through the fluid distribution module, and the automatic cleaning module is also used to provide a driving force so that the cleaning fluid located in the automatic cleaning module passes through the fluid distribution module into the reagent needle to clean the inner wall of the reagent needle and the connecting pipeline.

[0019] In some possible embodiments, the fluid storage module includes a sample storage unit, and the sample storage unit includes a sample needle; the automatic cleaning module is connected to the fluid driving module, and the fluid driving module or the automatic cleaning module is used to provide a driving force so that the cleaning fluid located in the automatic cleaning module enters the fluid driving module; the fluid driving module is also connected to the sample needle through the fluid distribution module, and the fluid driving module is also used to provide a driving force so that the cleaning fluid located in the fluid driving module passes through the fluid distribution module and enters the sample needle to clean the inner wall and connecting pipeline of the sample needle.

[0020] In some possible embodiments, the automatic cleaning module includes a third fluid distribution unit and a cleaning liquid storage unit that are interconnected, and the third fluid distribution unit includes a first flow direction selection component, a second flow direction selection component, a driving mechanism and a temporary storage pipeline. The first end of the temporary storage pipeline is connected to the first outlet of the first flow direction selection component and the first outlet of the second flow direction selection component, the second end of the temporary storage pipeline is connected to the fluid distribution module and the fluid driving module, the second outlet of the first flow direction selection component is connected to the reagent storage unit, and the common inlet of the second flow direction selection component is connected to the cleaning liquid storage unit. The two ends of the driving mechanism are respectively connected to the common inlet of the first flow direction selection component and the second outlet of the second flow direction selection component, and the driving mechanism is used to provide driving force, and the driving mechanism is also used to change the direction of fluid transport.

[0021] In some possible embodiments, a cleaning liquid selection unit is provided between the third fluid distribution unit and the cleaning liquid storage unit, and the cleaning liquid selection unit includes multiple inlets and a common outlet, the common outlet is connected to the main inlet of the third fluid distribution unit, and the multiple inlets are connected to the cleaning liquid storage unit.

[0022] In some possible embodiments, one inlet of the cleaning liquid selection unit is a gas port for communicating with gas.

[0023] In some possible embodiments, the fluid transport system further includes a waste liquid storage module, which is communicated with the fluid driving module and the fluid distribution module respectively.

[0024] In the second aspect, an embodiment of the present application provides another fluid transport system, including: a fluid storage module, a fluid usage module, a fluid driving module and an automatic cleaning module, wherein the fluid storage module is used to store fluid; the fluid usage module includes a flow channel, which is used to complete biochemical reactions, the liquid inlet end of the flow channel is connected to the fluid storage module, and the liquid outlet end of the flow channel is connected to the fluid driving module; the fluid driving module is used to generate a driving force so that the fluid located in the fluid storage module is loaded into the flow channel; the automatic cleaning module is used to clean the fluid transport system.

[0025] In some possible embodiments, the fluid storage module includes a reagent storage unit, the reagent storage unit includes a reagent needle, the automatic cleaning module is connected to the outer wall of the reagent needle, and the automatic cleaning module is also used to provide a driving force to transport the cleaning fluid located in the automatic cleaning module to the outer wall of the reagent needle to clean the outer wall of the reagent needle.

[0026] In some possible embodiments, the fluid storage module includes a reagent storage unit, the reagent storage unit includes a reagent needle, the automatic cleaning module is connected to the fluid driving module, the automatic cleaning module is used to provide a driving force so that the cleaning fluid located in the automatic cleaning module enters the fluid driving module, the fluid driving module is also connected to the reagent needle, and the fluid driving module is also used to provide a driving force so that the cleaning fluid located in the fluid driving module enters the reagent needle to clean the inner wall and connecting pipeline of the reagent needle.

[0027] In some possible embodiments, the fluid storage module includes a sample storage unit, and the sample storage unit includes a sample needle; the automatic cleaning module is connected to the fluid driving module, and the automatic cleaning module or the fluid driving module is used to provide a driving force so that the cleaning fluid located in the automatic cleaning module enters the fluid driving module, and the fluid driving module is also connected to the sample needle, and the fluid driving module is also used to provide a driving force so that the cleaning fluid located in the fluid driving module enters the sample needle to clean the inner wall and connecting pipeline of the sample needle.

[0028] In some possible embodiments, the automatic cleaning module includes a third fluid distribution unit and a cleaning liquid storage unit that are interconnected, and the third fluid distribution unit includes a first flow direction selection component, a second flow direction selection component, a driving mechanism and a temporary storage pipeline. The first end of the temporary storage pipeline is connected to the first outlet of the first flow direction selection component and the first outlet of the second flow direction selection component, the second end of the temporary storage pipeline is connected to the fluid distribution module and the fluid driving module, the second outlet of the first flow direction selection component is connected to the reagent storage unit, and the common inlet of the second flow direction selection component is connected to the cleaning liquid storage unit. The two ends of the driving mechanism are respectively connected to the common inlet of the first flow direction selection component and the second outlet of the second flow direction selection component, and the driving mechanism is used to provide driving force, and the driving mechanism is also used to change the direction of fluid transport.

[0029] In some possible embodiments, a cleaning liquid selection unit is provided between the third fluid distribution unit and the cleaning liquid storage unit, and the cleaning liquid selection unit includes multiple inlets and a common outlet, the common outlet is connected to the main inlet of the third fluid distribution unit, and the multiple inlets are connected to the cleaning liquid storage unit.

[0030] In some possible embodiments, one inlet of the cleaning liquid selection unit is a gas port for communicating with gas.

[0031] In some possible embodiments, the fluid usage module includes a plurality of the flow channels, and the fluid transport system further includes a fluid distribution module, which is respectively connected to the flow channels and the fluid storage module, and the fluid driving module is used to provide driving force so that the fluid located in the fluid storage module is independently loaded into each of the flow channels through the fluid distribution module.

[0032] In some possible embodiments, the fluid storage module includes a sample storage unit and a reagent storage unit, and the fluid distribution module includes a first fluid distribution unit and a second fluid distribution unit that are interconnected. The sample storage unit is connected to the flow channel through the first fluid distribution unit, and the reagent storage unit is connected to the flow channel through the second fluid distribution unit and the first fluid distribution unit. The first fluid distribution unit and the second fluid distribution unit are both used to control the opening or closing of the fluid pipeline according to a preset timing to select the transport direction of the fluid.

[0033] In some possible embodiments, the sample storage unit includes multiple sample containers, each of the flow channels is connected to the corresponding sample container through the first fluid distribution unit, and the driving force is used to enable the biological samples located in the sample containers to be independently loaded into the corresponding flow channels through the first fluid distribution unit.

[0034] In some possible embodiments, the second fluid dispensing unit is further connected to a designated sample container, and the driving force is further used to load the biological sample in the designated sample container into each of the flow channels sequentially through the second fluid dispensing unit and the first fluid dispensing unit.

[0035] In some possible embodiments, the first fluid dispensing unit is also connected to the fluid driving module, and the driving force is also used to load the reagent located in the reagent storage unit into the fluid driving module via the second fluid dispensing unit and the first fluid dispensing unit in sequence.

[0036] In a third aspect, an embodiment of the present application provides a fluid transport method, comprising:

[0037] Controlling the fluid distribution module to connect the flow channel in the fluid use module to the fluid storage module, wherein the fluid use module includes a plurality of independent flow channels, each of which is connected to the fluid storage module; and

[0038] A driving force is provided by a fluid driving module, so that the fluid in the fluid storage module is independently loaded into each of the flow channels via the fluid distribution module.

[0039] In some possible embodiments, the fluid storage module includes a sample storage unit, and the sample storage unit includes a plurality of sample containers.

[0040] The fluid transport method comprises:

[0041] controlling the fluid distribution module so that each of the flow channels is connected to one of the sample containers; and

[0042] The fluid driving module provides driving force for each of the flow channels, so that the biological sample in the sample container is independently loaded into the corresponding flow channel through the fluid distribution module.

[0043] In some possible embodiments, the fluid transport method further includes:

[0044] controlling the fluid distribution module so that all the flow channels communicate with a designated sample container; and

[0045] The fluid driving module provides a driving force so that the biological sample in the designated sample container is loaded independently or simultaneously into each flow channel through the fluid distribution module.

[0046] In some possible embodiments, the fluid storage module includes a reagent storage unit communicated with the fluid distribution module.

[0047] The fluid transport method comprises:

[0048] controlling the fluid dispensing module to connect the flow channel to the reagent storage unit; and

[0049] The fluid driving module provides a driving force so that the reagent in the reagent storage unit is loaded into the flow channel through the fluid dispensing module.

[0050] In some possible embodiments, the fluid transport method further includes:

[0051] controlling the fluid dispensing module so that the fluid driving module is in communication with the reagent storage unit through the fluid dispensing module; and

[0052] The fluid driving module provides a driving force so that the reagent in the reagent storage unit is loaded into at least the fluid pipeline between the fluid dispensing module and the fluid driving module.

[0053] In some possible embodiments, the fluid transport method further includes: automatically cleaning the fluid transport system.

[0054] In some possible embodiments, the fluid storage module includes a reagent storage unit, and the reagent storage unit includes a reagent needle.

[0055] The automatic cleaning of the fluid transport system includes: cleaning the outer wall of the reagent needle, including:

[0056] controlling the automatic cleaning module so that the automatic cleaning module is connected to the outer wall of the reagent needle; and

[0057] The automatic cleaning module provides a driving force so that the cleaning liquid in the automatic cleaning module is transported to the outer wall of the reagent needle to clean the outer wall of the reagent needle.

[0058] In some possible embodiments, the automatic cleaning of the fluid transport system further includes: cleaning the inner wall and connecting pipeline of the reagent needle, including the steps of:

[0059] controlling an automatic cleaning module so that the automatic cleaning module is in communication with the reagent needle through the fluid dispensing module; and

[0060] The automatic cleaning module provides driving force so that the cleaning fluid in the automatic cleaning storage module passes through the fluid dispensing module and enters the reagent needle to clean the inner wall and connecting pipeline of the reagent needle.

[0061] In some possible embodiments, the automatic cleaning of the fluid transport system includes: cleaning the connecting pipes and components along the path, including:

[0062] controlling the automatic cleaning module so that the automatic cleaning module is in communication with the fluid driving module; and

[0063] The automatic cleaning module or the fluid driving module provides driving force, so that the cleaning fluid in the automatic cleaning module enters the fluid driving module to clean the connecting pipelines and components on the path.

[0064] In some possible embodiments, the fluid storage module includes a sample storage unit, and the sample storage unit includes a sample needle.

[0065] The automatic cleaning of the fluid transport system includes: cleaning the inner wall of the sample needle and the connecting pipeline, including:

[0066] controlling the automatic cleaning module so that the automatic cleaning module is in communication with the fluid driving module;

[0067] Providing driving force through the fluid driving module or the automatic cleaning module so that the cleaning fluid in the automatic cleaning module enters the fluid driving module for temporary storage;

[0068] controlling the fluid dispensing module to connect the fluid driving module to the sample needle; and

[0069] The fluid driving module provides driving force so that the cleaning fluid temporarily stored in the fluid driving module enters the sample needle through the fluid distribution module to clean the inner wall and the connecting pipeline of the sample needle.

[0070] In some possible embodiments, the fluid transport method further includes:

[0071] The fluid driving module provides driving force to pump the waste liquid in the pipeline into the fluid driving module;

[0072] controlling the fluid driving module to communicate with the waste liquid storage module; and

[0073] The fluid driving module provides driving force to discharge the waste liquid in the fluid driving module into the waste liquid storage module.

[0074] In a fourth aspect, an embodiment of the present application provides a biochemical substance analysis device, which includes the fluid transport system as described above.

[0075] The fluid transport system provided by the embodiment of the present application integrates a fluid storage module, a fluid use module, a fluid distribution module and a fluid drive module into a biochemical substance analysis device. Through the mutual cooperation of the above structures, the function of the branch channel of the biochemical substance analysis device to independently load the same or different samples can be realized. The operation is simple and automatic loading can be achieved. In the process of independently loading samples in the branch channel, there is no need to use devices other than the equipment, which simplifies the structural complexity of the equipment, improves flexibility, reduces costs, and further reduces the operational difficulty of independently loading samples in the branch channel. Moreover, through the mutual cooperation of the above structures, the full-automatic cleaning function of the fluid transport system (including cleaning, emptying, drying and other processes) can be realized. Different cleaning fluids can be automatically replaced to clean different flow paths and components. The cleaning process is simple and does not require any manual intervention, which improves convenience. In addition, the modified fluid transport system can be adapted to existing biochemical substance analysis equipment to complete the automatic transportation of different biochemical reaction reagents. BRIEF DESCRIPTION OF THE DRAWINGS

[0076] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the following is a brief introduction to the drawings required for use in the embodiments of the present application. Obviously, the drawings described below are only some embodiments of the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work.

[0077] FIG1 is a schematic diagram of the system architecture of a fluid transportation system provided in one embodiment of the present application.

[0078] FIG2 is a schematic structural diagram of a fluid transport system provided in an embodiment of the present application.

[0079] FIG3 is a schematic structural diagram of a first fluid distribution unit provided in one embodiment of the present application.

[0080] FIG. 4 is a schematic diagram of the first fluid distribution unit in FIG. 3 in a first state.

[0081] FIG5 is a schematic diagram of the first fluid distribution unit in FIG3 in a second state.

[0082] FIG6 is a schematic diagram of the first fluid distribution unit in FIG3 in a third state.

[0083] FIG7 is a schematic structural diagram of a second fluid distribution unit provided in one embodiment of the present application.

[0084] FIG8 is a schematic structural diagram of a multi-liquid collecting block provided in one embodiment of the present application.

[0085] FIG9 is a schematic structural diagram of a cleaning liquid selection unit provided in an embodiment of the present application.

[0086] FIG10 is a schematic structural diagram of a fluid transport system provided in another embodiment of the present application.

[0087] FIG11 is a flow chart of a fluid transport method provided in one embodiment of the present application.

[0088] FIG12 is a schematic structural diagram of a fluid transport system provided in yet another embodiment of the present application.

[0089] FIG13 is a schematic structural diagram of the outer wall of an automatic cleaning reagent needle according to an embodiment of the present application.

[0090] FIG14 is a schematic structural diagram of transporting cleaning fluid to a temporary storage pipeline according to an embodiment of the present application.

[0091] FIG15 is a schematic structural diagram of another embodiment of the present application for transporting cleaning fluid to a temporary storage pipeline.

[0092] FIG16 is a schematic diagram of introducing air into a pipeline through a cleaning liquid selection unit according to an embodiment of the present application.

[0093] FIG17 is a schematic structural diagram of a biochemical substance analysis device provided in an embodiment of the present application.

[0094] Description of Main Component Symbols Fluid transport system 100 Second stator 323 Fluid storage module 10 Second rotor 324 Sample storage unit 101 Fourth communication port 325 Sample container 111 Common pipeline 303 Sample needle 112 Fluid drive module 40 Reagent storage unit 102 Fluid drive device 401 Reagent container 121 Drive assembly 402 Reagent needle 122 Reversing assembly 403 Reagent pre-treatment unit 103 First drive interface 431 Fluid use module 20 Second drive interface 432 Fluid use device 201 Third drive interface 433 Flow channel 211 Waste liquid storage module 50 Liquid inlet end A Automatic cleaning module 60 Liquid outlet end B Third fluid distribution unit 601 Temperature control device 202 First flow direction selection component 611 Fluid distribution module 30 Second flow direction selection component 612 First fluid distribution unit 301 Temporary pipeline 613 First interface 311 Drive mechanism 614 Second interface 312 Cleaning liquid storage unit 602 Third interface 313 Cleaning liquid container 621 Fourth interface 314 Cleaning liquid needle 622 First stator 315 Gas port 623 First rotor 316 Cleaning liquid selection unit 603 First communication port317 Three-way solenoid valve 631 Second communication port 318 Base manifold block 632 Third communication port 319 1-way multi-liquid collection block 605 Second fluid distribution unit 302 Control module 90 Main interface 321, 651 Biochemical substance analysis device 1000 Branch interface 322, 652 Imaging detection module 200 Second fluid distribution unit 302 Housing 300

[0095] The following specific implementation methods will further illustrate the present application in conjunction with the above-mentioned drawings. DETAILED DESCRIPTION

[0096] The following will be combined with the drawings in the embodiments of this application to clearly and completely describe the technical solutions in the embodiments of this application. Obviously, the embodiments described are only part of the embodiments of this application, not all of the embodiments. Based on the embodiments in this application, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of this application.

[0097] It should be noted that when a component is considered to be "disposed on" another component, it may be directly disposed on the other component or there may be a central component; when a component is considered to be "mounted on" another component, it may be directly mounted on the other component or there may be a central component. The term "and / or" as used herein includes all and any combinations of one or more of the relevant listed items.

[0098] Please refer to Figure 1, which is a schematic diagram of the system architecture of a fluid transport system 100 in one embodiment of the present application. The fluid transport system 100 can be used for the transportation of fluids, where the transported fluid can specifically be a liquid. It is understandable that the transported fluid can also be a gas. Specifically, the fluid transport system 100 can be used for the quantitative transportation of fluids in the process of biochemical substance analysis. For example, in the process of gene sequencing analysis, the fluid transport system 100 can be used for the transfer of biological samples and various reagents. The biological sample can be a human blood sample, a tissue sample, a saliva sample, or a molecular solution that has been pre-processed. It is understandable that the fluid transport system 100 can also be used for the transportation of fluids required for other experimental processes in a biochemical laboratory.

[0099] Please refer to Figure 2. The fluid transport system 100 includes a fluid storage module 10, a fluid use module 20 and a fluid drive module 40. The fluid storage module 10 is used to store fluids. Specifically, the fluids may include biological samples, various reagents required for biochemical reactions, cleaning reagents for cleaning fluid pipelines, water, and gases. The fluid use module 20 includes a flow channel 211, and biochemical reactions can be completed in the flow channel 211. The liquid inlet end A of the flow channel 211 is connected to the fluid storage module 10, and the liquid outlet end B is connected to the fluid drive module 40. The fluid drive module 40 is used to provide a driving force so that the fluid located in the fluid storage module 10 is loaded into the flow channel 211, thereby completing the biochemical reaction.

[0100] In some embodiments, the fluid utilization module 20 may include multiple independent flow channels 211. To independently load fluid into each flow channel 211, the fluid transport system 100 further includes a fluid distribution module 30. The liquid inlet A of each flow channel 211 is connected to the fluid storage module 10 via the fluid distribution module 30, and the liquid outlet B of each flow channel 211 is connected to the fluid drive module 40. The fluid distribution module 30 is used to select the direction of fluid transport. The fluid drive module 40 generates a driving force, allowing the fluid in the fluid storage module 10 to be independently loaded into each of the flow channels 211 via the fluid distribution module 30, thereby enabling each flow channel 211 in the fluid utilization module 20 to be independently loaded with fluid. It is understood that each flow channel 211 is independently loaded with fluid, and the same fluid or different fluids can be loaded. The fluid transport system 100 of this embodiment is particularly capable of independently loading different fluids into each flow channel 211. For example, different biological samples can be loaded into each flow channel 211 to perform different biochemical reactions, which is beneficial for improving fluid loading efficiency and increasing the flexibility of biochemical reactions within the fluid use module 20. It is also understood that the fluid drive module 40 can provide a separate driving force for each flow channel 211, thereby ensuring that each flow channel 211 does not interfere with each other during the independent fluid loading process.

[0101] 1 and 2 , the fluid storage module 10 may include a sample storage unit 101 and a reagent storage unit 102. The fluid dispensing module 30 is in communication with the sample storage unit 101 and the reagent storage unit 102, respectively. Driven by the fluid driving module 40, the fluid dispensing module 30 may selectively load the required fluid from the sample storage unit 101 and the reagent storage unit 102 for the fluid use module 20.

[0102] The sample storage unit 101 is used to store biological samples. The sample storage unit 101 may include a plurality of sample containers 111 and a plurality of sample needles 112. A sample needle 112 is provided above each sample container 111. The sample container 111 may be, for example, an independent cavity in a reagent bottle or a reagent kit, and each biological sample may be stored in a separate sample container 111. Specifically, each sample container 111 may correspond to a flow channel 211. The fluid distribution module 30 is connected to the sample storage unit 101 to connect each flow channel 211 and the corresponding sample needle 112. By selecting the flow direction of the fluid distribution module 30, the biological sample in a specific sample container 111 can be independently loaded into the corresponding flow channel 211, thereby realizing the independent loading of samples by the branch channel of the fluid use module 20, thereby improving the loading efficiency.

[0103] Among them, the reagent storage unit 102 can be used to store the reagents of the reaction class required for biochemical reactions, and can also be used to store the reagents of the cleaning class for cleaning pipelines and flow channels 211. The reagent storage unit 102 can include a reagent container 121 and a reagent needle 122, and a reagent needle 122 is provided above each reagent container 121. The reagent container 121 can be, for example, an independent cavity in a reagent bottle or a test kit. When multiple reagents are needed, each reagent can be stored in a separate reagent container 121. Specifically, the fluid distribution module 30 is also connected to the reagent storage unit 102. Through the flow direction selection of the fluid distribution module 30, the required reagents can be added to a specific flow channel 211, or the required reagents can be added to multiple flow channels 211 at the same time. It is also possible to realize targeted loading of reagents for different flow channels 211 according to the specific reaction process, thereby improving the flexibility of reagent loading and improving loading efficiency. In some embodiments, the reagent storage unit 102 can also be directly connected to the fluid drive module 40 without passing through the fluid distribution module 30.

[0104] In some embodiments, a reagent pre-treatment unit 103 is provided between the reagent storage unit 102 and the fluid dispensing module 30 for pre-treating the reagent drawn from the reagent storage unit 102 before the treated reagent enters the fluid dispensing module 30. The reagent pre-treatment unit 103 may specifically perform pre-treatment processes such as filtration and dissolved oxygen removal.

[0105] In some embodiments, the openings of the aforementioned sample container 111 and reagent container 121 may, but need not, be sealed with a puncturable sealing film (not shown). When fluid needs to be transported, the corresponding sample needle 112 and reagent needle 122 can be inserted into the interior of the aforementioned sample container 111 and reagent container 121 to absorb the fluid. In addition, the sample container 111 containing the biological sample is affixed with an identification code (such as a QR code or barcode, not shown). The identification code records the identification information of the corresponding biological sample, such as the name, age, test items, etc. of the test subject, for tracking and management of the biological sample. The amount of fluid in the sample container 111 and reagent container 121 can be quantitative. According to the needs of the biochemical reaction, the required amount of biological sample and reagent can be placed in the sample container 111 and reagent container 121 respectively in advance to achieve the purpose of quantitative pipetting. It is understandable that the amount of fluid absorbed can also be controlled by controlling the pressure gradient generated by the fluid drive module 40 to achieve the purpose of quantitative pipetting.

[0106] Referring again to Figures 1 and 2 , the fluid application module 20 includes a fluid application device 201, which includes multiple independent flow channels 211. Because each flow channel 211 in the fluid application device 201 can be independently loaded with fluid, the dimensions of the multiple flow channels 211 can be the same or different. During the fluid loading process, there is no need to consider whether the liquid injection time of each flow channel 211 is consistent or whether the reaction progress is consistent, thereby reducing operational difficulty and improving fluid loading efficiency and loading flexibility.

[0107] In some embodiments, the fluid utilization module 20 further includes a temperature control device 202, which can regulate the temperature within the flow channels 211 of the fluid utilization device 201, thereby enabling biochemical reactions of the fluid within the flow channels 211. It will be appreciated that the temperature control device 202 can independently control the temperature of each flow channel 211 to match the different reaction processes in different flow channels 211.

[0108] In some embodiments, the fluid-using device 201 may be, for example, a sequencing chip for performing gene sequencing reactions.

[0109] Please refer to Figures 1 and 2 again. The fluid distribution module 30 includes a first fluid distribution unit 301 and a second fluid distribution unit 302 that are interconnected. The first fluid distribution unit 301 and the second fluid distribution unit 302 are both used to control the opening or closing of the fluid pipeline according to a preset timing to select the direction of fluid flow. Among them, the first fluid distribution unit 301 is also connected to the fluid use module 20 and the sample storage unit 101 respectively. Under the drive of the fluid drive module 40, the biological sample located in the sample storage unit 101 can be independently loaded into the corresponding flow channel 211 of the fluid use module 20 through the selection of the first fluid distribution unit 301. The second fluid distribution unit 302 is also connected to the reagent storage unit 102. Under the drive of the fluid drive module 40, the reagents for biochemical reactions located in the reagent storage unit 102 can be loaded into the flow channel 211 of the fluid use module 20 in sequence through the second fluid distribution unit 302 and the first fluid distribution unit 301 to complete the biochemical reaction. It is understandable that the same reagent can be loaded simultaneously into multiple flow channels 211, or the required reagent can be loaded individually into a specific flow channel 211. Similarly, under the drive of the fluid driving module 40, the cleaning reagents in the reagent storage unit 102 can be loaded into the flow channel 211 of the fluid using module 20 and the fluid pipeline that needs to be cleaned through the second fluid dispensing unit 302 and the first fluid dispensing unit 301 in sequence, thereby completing the filling of the flow channel 211 and the fluid pipeline.

[0110] 3 , the first fluid dispensing unit 301 may include multiple interfaces, including a first interface 311 connected to the flow channel 211, a second interface 312 connected to the fluid drive module 40, a third interface 313 connected to the sample storage unit 101, and a fourth interface 314 connected to the second fluid dispensing unit 302. The first interface 311, the second interface 312, the third interface 313, and the fourth interface 314 are normally disconnected. When a certain pipeline needs to transfer fluid, the corresponding interface is opened. For example, connecting the first interface 311 and the third interface 313 can achieve communication between the flow channel 211 and the corresponding sample container 111; connecting all the first interfaces 311 and the fourth interface 314 can achieve communication between the flow channel 211 and the reagent container 121; and connecting the second interface 312 and the fourth interface 314 can achieve communication between the fluid drive module 40 and the reagent container 121. In some embodiments, the number of first interfaces 311 can be set according to the number of flow channels 211, with each flow channel 211 connected to a first interface 311. The number of third interfaces 313 can be set according to the number of sample containers 111, with each sample container 111 connected to a third interface 313. In some embodiments, there can be one second interface 312 connected to the fluid drive module 40, and one fourth interface 314 connected to the second fluid dispensing unit 302.

[0111] In some embodiments, the first fluid dispensing unit 301 includes a stacked first stator 315 and a first rotor 316. The first interface 311, the second interface 312, the third interface 313, and the fourth interface 314 are all disposed on the first stator 315. The first rotor 316 is provided with a first communication port 317, a second communication port 318, and a third communication port 319. By rotating the first rotor 316, the relative position of the first rotor 316 and the first stator 315 can be changed, and the interfaces that need to be connected among the first interface 311, the second interface 312, the third interface 313, and the fourth interface 314 can be selected for connection, thereby enabling the fluid use module 20 to load biological samples, reagents, or cleaning fluids. Specifically, the first fluid dispensing unit 301 can be a special-shaped rotary valve.

[0112] Specifically, the first fluid dispensing unit 301 can have three communication states depending on the connectivity between different interfaces. As shown in Figures 2 and 4, the first communication port 317 is used to connect the first interface 311 and the corresponding third interface 313. At this time, the first rotor 316 and the first stator 315 are in the first state, allowing the biological sample located in the sample container 111 to enter the corresponding flow channel 211 via the third interface 313 and the first interface 311, thereby enabling independent sample loading for each flow channel 211. As shown in Figures 2 and 5, the second communication port 318 is used to connect the first interface 311 and the fourth interface 314. At this time, the first rotor 316 and the first stator 315 are in the second state, allowing the reagent located in the reagent container 121 to enter the corresponding flow channel 211 via the fourth interface 314 and the first interface 311, thereby enabling reagent loading for the flow channel 211 or cleaning the flow channel 211 and the fluid pipeline. As shown in Figures 2 and 6, the third connecting port 319 is used to connect the second interface 312 and the fourth interface 314. At this time, the first rotor 316 and the first stator 315 are in the third state, so that the reagent located in the reagent container 121 can enter the fluid driving module 40 or enter the common pipeline 303 between the first fluid distribution unit 301 and the second fluid distribution unit 302 through the fourth interface 314 and the second interface 312, thereby filling the common pipeline 303.

[0113] Please refer to Figure 2 again. The second fluid distribution unit 302 includes a main interface 321 and multiple sub-interfaces 322. The main interface 321 is connected to the fourth interface 314 of the first fluid distribution unit 301, and the sub-interfaces 322 can be connected to corresponding reagent containers 121. The main interface 321 is connected to the corresponding sub-interfaces 322 according to a preset timing control, so that corresponding reagents can be added to the fluid use module 20 and fluid pipelines.

[0114] In some embodiments, as shown in conjunction with FIG. 7 , the second fluid dispensing unit 302 may include a second stator 323 and a second rotor 324. The main interface 321 and the tap interface 322 are both located on the second stator 323, and the second rotor 324 is provided with a fourth communication port 325 for connecting the main interface 321 with corresponding tap interfaces 322. In some embodiments, the second stator 323 includes a single main interface 321 and multiple tap interfaces 322, and the second rotor 324 includes a single fourth communication port 325. Rotating the second rotor 324 changes the relative position of the second rotor 324 and the second stator 323, thereby enabling different tap interfaces 322 to communicate with the main interface 321 via the fourth communication port 325, thereby transferring the corresponding reagent to the first fluid dispensing unit 301. Specifically, the second fluid dispensing unit 302 may be a rotary valve.

[0115] Please refer to Figures 1 and 2 again. The fluid drive module 40 is used to generate a driving force to achieve the movement of the fluid in the fluid transport system 100. In some embodiments, the fluid drive module 40 can also accurately quantify the fluid to achieve the purpose of quantitatively loading the fluid. One form of the driving force can be pressure, including negative pressure and positive pressure, depending on the direction of fluid transport. Among them, the fluid drive module 40 may include a plurality of fluid drive devices 401, wherein each fluid drive device 401 can be connected to a flow channel 211, and each fluid drive device 401 can also be connected to the fluid distribution module 30 to achieve the purpose of independently loading samples for each flow channel 211, and also to achieve the purpose of loading reagents and cleaning fluid lines for the flow channel 211. In addition, the fluid drive device 401 also has the function of fluid caching to facilitate adjustment of the transport direction of the fluid.

[0116] In some embodiments, the fluid drive device 401 includes a drive assembly 402 and a reversing assembly 403 connected to the drive assembly 402. Among them, the drive assembly 402 can achieve quantitative drive, for example, a drive pump with a scale can be used to achieve the purpose of quantitatively transferring fluid. In addition, the drive assembly 402 can also be provided with a channel for caching the fluid so as to adjust the transport direction of the fluid. The reversing assembly 403 is used to switch the transport direction of the fluid to adjust the communication between the drive assembly 402 and different pipelines, for example, to enable the fluid to be drawn into the channel of the drive assembly 402, and also to enable the fluid to be discharged from the channel of the drive assembly 402.

[0117] In some embodiments, the drive assembly 402 can be a hydraulic unit, such as a peristaltic pump, a plunger pump, a syringe pump, a gear pump, or a diaphragm pump, or can be an air power unit, such as a vacuum pump, a pneumatic pipette, a diaphragm pump, or an air compressor.

[0118] In some embodiments, the reversing assembly 403 may be a reversing valve, including multiple interfaces, such as a first drive interface 431 connected to the corresponding flow channel 211, a second drive interface 432 connected to the first fluid distribution unit 301, and a third drive interface 433. The third drive interface 433 may be used to reset the drive assembly 402. For example, the third drive interface 433 may be connected to the outside world to reset the drive assembly 402. Specifically, the reversing assembly 403 may be a reversing valve, wherein the reversing valve may be a solenoid valve, a rotary valve, a pneumatic reversing valve, an electro-hydraulic reversing valve, a manual reversing valve, a piezoelectric valve, a pinch valve, a rotary valve, or a rotary cutting valve.

[0119] In some embodiments, the fluid driving module 40 further includes a fluid monitoring device (not shown), which may include a pressure sensor, a flow sensor, a bubble sensor, and the like.

[0120] Referring again to Figures 1 and 2 , the fluid transport system 100 further includes a waste liquid storage module 50 for storing or discharging all or part of the waste liquid from the fluid transport system 100. The fluid drive module 40 is further configured to generate a driving force to transfer waste liquid within the flow channel 211 or within the fluid pipeline to the waste liquid storage module 50. The waste liquid storage module 50 can be in communication with the fluid distribution module 30. Driven by the fluid drive module 40, the waste liquid in the flow channel 211 and the fluid pipeline is discharged to the waste liquid storage unit 104 via the fluid distribution module 30. In some embodiments, the waste liquid storage module 50 is connected to a tapping port 322 of the second fluid distribution unit 302. Furthermore, the waste liquid storage module 50 can also communicate with the fluid drive module 40. The fluid drive module 40 is used to generate a negative pressure driving force to initially transfer waste liquid within the flow channel 211 and the fluid pipeline into the fluid drive module 40. The fluid drive module 40 is then used to generate a positive pressure driving force to transfer waste liquid within the fluid drive module 40 to the waste liquid storage module 50 to achieve waste liquid discharge. Specifically, the third drive interface 433 of the reversing assembly 403 can communicate with the waste liquid storage module 50, which can not only discharge waste liquid but also reset the drive assembly 402.

[0121] In some embodiments, the waste liquid storage module 50 may be internal or external to the biochemical substance analysis system, or the waste liquid storage module 50 may be provided with a waste liquid interface, which is used to directly discharge waste liquid into the laboratory waste liquid treatment system. It is understood that the waste liquid storage module 50 may not contain any container, or may include one or more containers. It is also understood that, depending on functional requirements, the waste liquid storage module 50 may include sensors for liquid monitoring, air purification equipment, or secondary overflow prevention equipment.

[0122] Referring again to Figures 1 and 2 , the fluid transport system 100 also includes an automatic cleaning module 60, which is configured to communicate with other components of the fluid transport system 100 and transport cleaning fluid to the components requiring cleaning, thereby automatically cleaning the fluid transport system 100. Specifically, the automatic cleaning module 60 includes a third fluid dispensing unit 601 and a cleaning fluid storage unit 602. The cleaning fluid storage unit 602 is configured to store cleaning fluid, which may be, for example, a cleaning reagent, an alkaline solution, or water. The third fluid dispensing unit 601 may have a flow direction selector, allowing it to select the direction of the cleaning fluid flow, thereby enabling cleaning of different components. For example, it may simultaneously clean multiple flow channels 211 and multiple fluid lines, clean specific flow channels 211 and fluid lines, clean the reagent needle 122, clean the sample needle 112, clean the bypass, and so on. Furthermore, the third fluid dispensing unit 601 may also provide power for transporting the cleaning fluid, allowing for flexible cleaning of various components without being limited by the inconvenient connection of the fluid drive module 40.

[0123] Among them, the third fluid distribution unit 601 includes a first flow direction selection component 611, a second flow direction selection component 612, a driving mechanism 614 and a temporary storage pipeline 613. The first end of the temporary storage pipeline 613 is connected to the first outlet of the first flow direction selection component 611 and the first outlet of the second flow direction selection component 612, and the second end of the temporary storage pipeline 613 is connected to the fluid storage module 10, the fluid use module 20 and the fluid driving module 40. The second outlet of the first flow direction selection component 611 is connected to the reagent storage unit 102, and the common inlet of the second flow direction selection component 612 is connected to the cleaning liquid storage unit 602. The two ends of the driving mechanism 614 are respectively connected to the common inlet of the first flow direction selection component 611 and the second outlet of the second flow direction selection component 612. The driving mechanism 614 is used to provide driving force, and the driving mechanism 614 can change the direction of fluid transport.

[0124] In some embodiments, the first flow direction selection component 611 can be a three-way solenoid valve DT7, the second flow direction selection component 612 can be a three-way solenoid valve DT8, and the drive mechanism 614 can be a diaphragm pump. One outlet of each of the three-way solenoid valve DT7 and the three-way solenoid valve DT8 is connected to a temporary storage pipeline 613, which is further connected to the fluid drive module 40 and the fluid distribution module 30. A stop valve DT6 is provided between the temporary storage pipeline 613, the fluid drive module 40 and the fluid distribution module 30. The opening or closing of the stop valve DT6 can connect or disconnect the relevant pipelines. The drive mechanism 614 can rotate forward and reverse. The forward fluid direction is from the three-way solenoid valve DT8 to the three-way solenoid valve DT7, and the reverse fluid direction is from the three-way solenoid valve DT7 to the three-way solenoid valve DT8.

[0125] In some embodiments, one outlet of the first flow direction selection component 611 (specifically, the three-way solenoid valve DT7 ) is connected to the top of the outer wall of the reagent needle 122 through a pipeline, so that the outer wall of the reagent needle 122 can be cleaned.

[0126] In some embodiments, a one-point multi-liquid collection block 605 is provided between the automatic cleaning module 60 (specifically, the first flow direction selection component 611) and the multiple reagent needles 122. The one-point multi-liquid collection block 605 includes a main interface 651 connected to an outlet of the automatic cleaning module 60 and a branch interface 652 connected to the outer wall of the reagent needle 122. For example, it can be a one-point 18-liquid collection block (as shown in FIG8 ), which can be connected to multiple reagent needles 122 to clean the outer wall of each reagent needle 122, thereby improving cleaning efficiency.

[0127] The cleaning liquid storage unit 602 may include a cleaning liquid container 621 and a cleaning liquid needle 622, with a cleaning liquid needle 622 located above each cleaning liquid container 621. The cleaning liquid container 621 may be, for example, a reagent bottle or an independent cavity in a reagent kit. When multiple cleaning liquids are required, each cleaning liquid may be stored in a separate cleaning liquid container 621. In some embodiments, the reagent container 121 containing cleaning reagents in the reagent storage unit 102 may be integrated with the cleaning liquid container 621 in the cleaning liquid storage unit 602 and placed as an external structure outside the instrument to facilitate replacement or addition of cleaning reagents and cleaning liquids.

[0128] In some embodiments, the cleaning fluid storage unit 602 may further include a gas port 623 for providing gas (eg, air). Air is input into the fluid transport system 100 to empty the liquid in the pipeline.

[0129] In some embodiments, a cleaning liquid selection unit 603 is further provided between the third fluid dispensing unit 601 and the cleaning liquid storage unit 602. The cleaning liquid selection unit 603 includes multiple inlets and a common outlet, each of which is connected to a cleaning liquid container 621. The common outlet is connected to the main inlet of the third fluid dispensing unit 601, specifically to the common inlet of the three-way solenoid valve DT8. By providing the cleaning liquid selection module 80 between the third fluid dispensing unit 601 and the cleaning liquid storage unit 602, the desired cleaning liquid can be flexibly selected.

[0130] In some embodiments, the cleaning liquid selection unit 603 can specifically be a two-way solenoid valve group, and one inlet of the two-way solenoid valve group can serve as the gas port 623 of the cleaning liquid storage unit 602, thereby inputting gas (such as air) into the fluid pipeline to discharge the liquid in the fluid pipeline.

[0131] In some embodiments, one inlet of the cleaning liquid selection unit 603 can be connected to the waste liquid storage module 50, thereby facilitating the discharge of waste liquid from the fluid pipeline. Specifically, the cleaning liquid selection unit 603 can be a two-way solenoid valve assembly, which can be composed of multiple three-way solenoid valves 631 and a base manifold block 632, as shown in Figure 9.

[0132] 1 again, the fluid transport system 100 further includes a control module 90 for controlling the coordinated operation of the fluid storage module 10, the fluid use module 20, the fluid distribution module 30, the fluid drive module 40, and the automatic cleaning module 60.

[0133] Referring to FIG. 10 , in another embodiment, the sample storage unit 101 in the fluid transport system 100 is further connected to the second fluid dispensing unit 302. Specifically, a sample container 111 in the sample storage unit 101 can be connected to the first fluid dispensing unit 301 and the second fluid dispensing unit 302, respectively. Communication with the first fluid dispensing unit 301 allows independent loading of samples into corresponding flow channels 211, while communication with the second fluid dispensing unit 302 allows the addition of the same sample to multiple flow channels 211. In some embodiments, a sample container 111 in the sample storage unit 101 is connected to the first fluid dispensing unit 301 and the second fluid dispensing unit 302, respectively, via a three-way solenoid valve DT9.

[0134] Please refer to FIG. 11 , and refer to FIG. 1 and FIG. 2 together, for a method of transporting fluid using the aforementioned fluid transport system 100 , which specifically includes the following steps:

[0135] Step S1 , controlling the fluid distribution module 30 to connect the flow channel 211 in the fluid use module 20 with the fluid storage module 10 , wherein the fluid use module 20 includes multiple independent flow channels 211 , and each flow channel 211 is connected with the fluid storage module 10 .

[0136] In step S2 , the fluid driving module 40 provides a driving force so that the fluid in the fluid storage module 10 is independently loaded into each of the flow channels 211 via the fluid distribution module 30 .

[0137] In step S1, in some embodiments, when it is necessary to independently load fluid into each flow channel 211, each independent flow channel 211 can be connected to a separate container in the fluid storage module 10 via the fluid distribution module 30, thereby independently loading fluid into each flow channel 211. It is understood that when independently loading fluid into the flow channels 211, fluid can be loaded into only a specific flow channel 211 that needs to be loaded, or fluid can be loaded into multiple flow channels 211 simultaneously. It is also understood that each independent flow channel 211 can be loaded with the same type of fluid or different types of fluid.

[0138] In step S1, in other embodiments, when it is necessary to load the same fluid into multiple flow channels 211, the fluid distribution module 30 can also be used to simultaneously connect multiple independent flow channels 211 to the same container of the fluid storage module 10, thereby achieving the loading of the same fluid into multiple independent flow channels 211.

[0139] In step S2, in some embodiments, the fluid driving module 40 includes multiple fluid driving devices 401, and each flow channel 211 is independently connected to a fluid driving device 401. The fluid driving device 401 can independently provide driving force for each flow channel 211, so as to adjust the size of the driving force and the timing of fluid loading, and then control the amount of fluid that needs to be loaded into each flow channel 211 or the loading speed and loading time of the fluid, etc.

[0140] After step S2, the fluid transport method further comprises:

[0141] Step S3: The fluid driving module 40 is connected to the waste liquid storage module 50, and the fluid driving module 40 provides driving force to discharge the waste liquid in the fluid driving module 40 into the waste liquid storage module 50. Specifically, during the process of fluid loading in the flow channel 211, excess reaction fluid (old fluid) will remain in the flow channel 211. At the same time, in order to fill the flow channel 211 with fluid, some excess fluid will flow out from the liquid outlet B of the flow channel 211. This old fluid and the excess new fluid enter the fluid driving module 40 to form the aforementioned waste liquid. Step S3 is to empty the waste liquid in the fluid driving module 40.

[0142] 1. The fluid transport method for independently loading fluid into each flow channel 211 (i.e., independently loading the branch flow channels) includes (see FIG. 2 and FIG. 4 ):

[0143] Step S1a: controlling the fluid distributing module 30 so that each flow channel 211 in the fluid using module 20 is connected to each sample container 111 of the sample storage unit 101 .

[0144] Specifically, each sample container 111 is connected to the corresponding flow channel 211 through the first fluid dispensing unit 301. In this step, the first rotor 316 of the first fluid dispensing unit 301 is controlled to rotate so that the first interface 311 is connected to the corresponding third interface 313, thereby connecting each flow channel 211 to the corresponding sample container 111.

[0145] In step S2a, the fluid driving device 401 provides driving force to each of the flow channels 211, so that the fluid in each sample container 111 is independently loaded into the corresponding flow channel 211 via the fluid distribution module 30. It is understood that at this point, all of the fluid driving devices 401 can be controlled simultaneously to provide driving force to each of the flow channels 211 to enable independent sample loading for each of the flow channels 211, or only a specific fluid driving device 401 can be controlled to load the sample into a specific flow channel 211.

[0146] After step S2a, the fluid transport method further comprises:

[0147] Step S3a: The fluid driving module 40 is connected to the waste liquid storage module 50, and the fluid driving module 40 provides a driving force to discharge the waste liquid in the fluid driving module 40 into the waste liquid storage module 50. Specifically, during the process of loading the sample into the flow channel 211, in order to fill the flow channel 211 with the sample, some excess sample will enter the fluid driving module 40 from the liquid outlet B of the flow channel 211 to constitute the aforementioned waste liquid. Step S3a is to empty the aforementioned waste liquid from the fluid driving module 40.

[0148] Specifically, the detailed process of independently loading samples in the branch channel is as follows:

[0149] Step a. Different biological samples are placed in different sample containers 111 of the sample storage unit 101 .

[0150] Step b (loading the sample into the flow channel 211): As shown in Figure 4, the first rotor 316 of the first fluid distribution unit 301 (for example, a special-shaped rotary valve) is rotated to the position of the first state, and the reversing component 403 of each fluid drive device 401 in the fluid drive module 40 is switched to the first drive interface 431. The fluid drive component 402 (for example, an injection pump) is downward to provide suction power. A certain volume of the biological sample is extracted under negative pressure drive, and passes through the sample needle 112 and the pipeline, the first interface 311 and the third interface 313 of the first fluid distribution unit 301 in sequence, and is independently loaded into the flow channel 211 from the liquid inlet end A of the corresponding flow channel 211, thereby completing the independent sample loading of each flow channel 211.

[0151] Step c (resetting the drive assembly 402): As shown in Figure 6, the first rotor 316 of the first fluid distribution unit 301 is rotated to the third state position, and the second rotor 324 of the second fluid distribution unit 302 (for example, a rotary valve) is rotated to rotate the fourth connecting port 325 to the branch interface 322 connected to the waste liquid storage module 50, so that the second fluid distribution unit 302 is connected to the waste liquid storage module 50, and the reversing assembly 403 of the fluid drive device 401 is switched to the third drive interface 433, and the drive assembly 402 moves upward, so that the piston of the drive assembly 402 is reset to the top position.

[0152] Step d (exhausting air from the fluid pipeline): As shown in FIG6 , the first rotor 316 of the first fluid dispensing unit 301 remains in the third state. At this time, the second rotor 324 of the second fluid dispensing unit 302 (e.g., a rotary valve) rotates, causing the fourth communication port 325 to rotate to the tapping port 322 connected to a reagent container 121 of the reagent storage unit 102, thereby connecting the second fluid dispensing unit 302 to the reagent storage unit 102. Subsequently, the reversing assembly 403 switches to the second drive interface 432, connecting the fluid drive device 401 to the first fluid dispensing unit 301. The drive assembly 402 moves downward to provide suction power, and a reagent in the reagent storage unit 102 is extracted, passing through the reagent needle 122, the second fluid dispensing unit 302, and the first fluid dispensing unit 301 in sequence and entering the channel of the drive assembly 402.

[0153] Step e (clearing and resetting the drive assembly 402): the first rotor 316 of the first fluid dispensing unit 301 remains in the third state position, and the second fluid dispensing unit 302 is still connected to the reagent storage unit 102; the reversing assembly 403 switches to the third drive interface 433, so that the fluid drive device 401 is connected to the waste liquid storage module 50, and the drive assembly 402 provides a driving force for the upward movement. The reagent in the channel of the drive assembly 402 in the previous step is pushed out and enters the waste liquid storage module 50 through the third drive interface 433.

[0154] Step f (loading reagents into the flow channel 211): As shown in Figure 5, the second fluid dispensing unit 302 is still connected to the reagent storage unit 102. At this time, the first rotor 316 of the first fluid dispensing unit 301 is rotated to the second state position, and the second connecting port 318 connects all the third interfaces 313 with the fourth interface 314. The reversing component 403 of the fluid driving device 401 is switched to the first driving interface 431. The driving component 402 moves downward to provide suction power. The required reagent in the reagent storage unit 102 is extracted and enters each flow channel 211 from the liquid inlet end A of the flow channel 211 through the second fluid dispensing unit 302 and the first fluid dispensing unit 301 to replace the reagent in the flow channel 211. The replaced reagent and excess reagent are discharged from the liquid outlet end B of the flow channel 211 and temporarily stored in the channel of the driving component 402.

[0155] Step g (clearing and resetting the drive assembly 402 ): the reversing assembly 403 switches to the third drive interface 433 , the drive assembly 402 provides a driving force to move upward, and the reagent temporarily stored in the drive assembly 402 in the previous step is pushed out and enters the waste liquid storage module 50 .

[0156] The purpose of step d is to exhaust the air in the fluid channel with the reagent. In addition, the above steps describe the situation where all the flow channels 211 are loaded with samples, and samples can also be selectively loaded for one or more independent flow channels 211.

[0157] 2. The fluid transport method for loading the same sample into all flow channels 211 includes (please refer to FIG. 10 and FIG. 5 in conjunction with):

[0158] Step S1b: controlling the fluid distribution module 30 to connect all the flow channels 211 to a designated sample container 111 .

[0159] Specifically, a tap 322 of the second fluid dispensing unit 302 is connected to a designated sample container 111 , and the first fluid dispensing unit 301 is rotated to the second state, where the second fluid dispensing unit 302 is connected to the first fluid dispensing unit 301 , and the first fluid dispensing unit 301 is connected to all flow channels 211 .

[0160] In step S2 b , the fluid driving module 40 provides a driving force so that the biological sample in the designated sample container 111 is loaded independently or simultaneously into each flow channel 211 through the fluid distributing module 30 .

[0161] Specifically, when each flow channel 211 needs to be loaded with the same sample at the same time, each driving component 402 of the fluid driving module 40 can be controlled to increase the driving force for each flow channel 211. When only part of the flow channels 211 needs to be loaded with the same sample, part of the driving component 402 can also be rotated to move, thereby flexibly adjusting the sample loading method.

[0162] Specifically, the detailed process of loading the same sample in multiple channels includes the following steps:

[0163] As shown in step a., all samples are placed in one channel in the sample storage unit 101 , for example, the fourth sample container 111 .

[0164] Step b. The three-way solenoid valve DT9 is energized, a sample needle 112 is connected to a tap 322 of the second fluid dispensing unit 302, and the second rotor 324 of the second fluid dispensing unit 302 is rotated to rotate the fourth communication port 325 to the tap 322 connected to the sample needle 112;

[0165] Step c. The first rotor 316 of the first fluid dispensing unit 301 rotates to the third state position, so that the first fluid dispensing unit 301 is connected to the aforementioned branch interface 322 of the second fluid dispensing unit 302. Thereafter, the reversing component 403 of the fluid drive device 401 is switched to the second drive interface 432. The drive component 402 is driven downward to provide suction power. The sample in the designated sample container 111 (for example, the fourth sample container) in the sample storage unit 101 is extracted by a certain volume under negative pressure. The sample passes through the sample needle 112, the three-way solenoid valve DT9, the second fluid dispensing unit 302 in sequence, and finally reaches the first fluid dispensing unit 301. At this time, the pipe connecting the sample needle 112 to the flow channel 211 is filled with the sample to be tested. The purpose of this step is to clean the fluid pipeline and fill the fluid pipeline with the sample.

[0166] Step d. The first rotor 316 of the first fluid dispensing unit 301 is maintained in the second state position, the reversing assembly 403 is switched to the first drive interface 431, and the drive assembly 402 is downwardly driven to provide suction power. Driven by negative pressure, a certain volume of biochemical sample is extracted from the sample container 111. The sample filled in the fluid pipeline in the previous step c will enter each independent flow channel 211 from the liquid inlet end A of the flow channel 211, and the excess sample will be discharged from the liquid outlet end B of the flow channel 211 to the drive assembly 402 for temporary storage, thereby completing the loading of the same biochemical sample into multiple independent flow channels 211.

[0167] Step e. Switch the reversing component 403 to the third drive interface 433 , and drive the component 402 upward to provide a driving force to push the biochemical sample temporarily stored in the drive component 402 in step d into the waste liquid storage module 50 .

[0168] 3. Transportation of Reagents

[0169] 1. The fluid transport method for loading reagents into the flow channel 211 (i.e., the reagent by-chip path, which may be referred to as the "by-chip" path) includes (see FIG. 12 in conjunction with FIG. 5 ):

[0170] Step S1c: controlling the fluid dispensing module 30 to connect the flow channel 211 to the reagent storage unit 102 .

[0171] Specifically, the flow channel 211 is connected to the reagent storage unit 102 through the first fluid dispensing unit 301 and the second fluid dispensing unit 302 .

[0172] In step S2c, the fluid driving device 401 provides driving force so that the reagent in the reagent storage unit 102 is loaded into the corresponding flow channel 211 via the fluid dispensing module 30 .

[0173] It is understandable that at this time, all fluid driving devices 401 can be controlled simultaneously to provide driving force for each flow channel 211 to achieve the loading of reagents in each flow channel 211, or only a specific fluid driving device 401 can be controlled to load reagents for a specific flow channel 211.

[0174] It is understandable that when the flow channel 211 is loaded with reagent, there may be old reagent in the flow channel 211. At the same time, in order to fill the flow channel 211, an excess of new reagent may be required. In this way, the old reagent and part of the new reagent will be temporarily stored in the fluid driving module 40. Therefore, after step S2c, the following steps are further included:

[0175] Step S3c: connecting the fluid driving module 40 to the waste liquid storage module 50 , and providing driving force through the fluid driving module 40 to discharge the reagent in the fluid driving module 40 into the waste liquid storage module 50 .

[0176] The implementation of the [bypass] transport path is described as follows:

[0177] Step a. The second fluid dispensing unit 302 is switched to the pipeline connection branch interface 322 of the required reagent (if the required reagent is reagent 1 in the external cleaning tank, DT13 in the two-way solenoid valve group needs to be kept in the closed state), the first rotor 316 of the first fluid dispensing unit 301 rotates to the third state position, the reversing component 403 switches to the second drive interface 432, and the drive component 402 moves downward to provide suction power. A certain volume of the required reagent passes through the reagent needle 122, the reagent pretreatment device, the second fluid dispensing unit 302, and the first fluid dispensing unit 301 in sequence. At this time, the reagent can enter the channel of the drive component 402 for temporary storage, or it can just exceed the second interface 312 of the first fluid dispensing unit 301.

[0178] Step b. Switch the reversing component 403 to the third drive interface 433, and the drive component 402 provides a driving force to push the reagent in the drive component 402 in step a into the waste liquid storage module 50; if the reagent does not enter the drive component 402, switch the reversing component 403 to the third drive interface 433, and the drive component 402 provides a driving force to reset the drive component 402.

[0179] 2. Before loading one fluid, or between loading two different fluids, the fluid transport method further includes: cleaning the common pipeline 303 between the first fluid distribution unit 301 and the second fluid distribution unit 302 (ie, bypass cleaning).

[0180] Fluid transport methods for bypass cleaning (which may be named "bypass" path) include (please refer to Figure 12 again, in conjunction with Figure 6):

[0181] Step S1d: controlling the fluid dispensing module 30 so that the fluid driving module 40 is connected to the reagent storage unit 102 through the fluid dispensing module 30 .

[0182] In step S2d, the fluid driving module 40 provides a driving force so that the reagent in the reagent storage unit 102 is at least loaded into the fluid pipeline between the fluid driving module 40 and the fluid dispensing module 30 .

[0183] Specifically, the reagent can be loaded at least into the common pipeline 303 between the first fluid dispensing unit 301 and the second fluid dispensing unit 302, and exceed the first fluid dispensing unit 301 to enter the part of the pipeline connected to the fluid driving module 40, so as to ensure that the reagent can fill the first fluid dispensing unit 301, the second fluid dispensing unit 302 and the common pipeline 303 to discharge the gas or old reagent in the pipeline.

[0184] It is understandable that, during the bypass cleaning process, after step S2d, the following steps are also included:

[0185] In step S3d, the fluid drive module 40 is connected to the waste liquid storage module 50, and the fluid drive module 40 provides driving force, so that at least the fluid pipeline located between the fluid drive module 40 and the fluid distribution module 30 further enters the fluid drive module 40, and the reagent located in the fluid drive module 40 is discharged into the waste liquid storage module 50. In other words, the old fluid (which may also include some new fluid) in the common pipeline 303 can be sucked into the fluid drive module 40 for temporary storage and further discharged into the waste liquid storage module 50.

[0186] The implementation of the [bychip] transport path is described as follows:

[0187] Step a. Switch the second rotor 324 of the second fluid dispensing unit 302 to the pipeline connection branch interface 322 of the required reagent (if the required reagent is reagent 1 in the external cleaning tank, DT13 in the two-way solenoid valve group needs to be kept in the closed state), the first rotor 316 of the first fluid dispensing unit 301 rotates to the second state position, the reversing component 403 switches to the first drive interface 431, and the drive component 402 moves downward to provide suction power. A certain volume of the required reagent passes through the reagent needle 122, the reagent pretreatment device, the second fluid dispensing unit 302, and the first fluid dispensing unit 301 in sequence, and enters the flow channel 211 from the liquid inlet end A of the flow channel 211, replacing the old reagent in the flow channel 211. The old reagent and excess new reagent are discharged from the liquid outlet end B of the flow channel 211 to the channel of the drive component 402 for temporary storage.

[0188] Step b: switching the reversing component 403 to the third drive interface 433 , and driving the component 402 upward to provide a driving force to push the reagent temporarily stored in the driving component 402 in step a into the waste liquid storage module 50 .

[0189] As you can understand, there are many ways to combine the aforementioned [bypass] path and [bychip] path:

[0190] The first method: first perform the [bypass] path, then the [bychip] path.

[0191] The second method is to first perform steps S1d and S2d in the [bypass] path, then perform steps S1c and S2c in the [bychip] path, and then perform step S3c to empty the waste liquid stored in the fluid driving module 40 after the two pumping operations.

[0192] The third method: first perform step S1d and step S2d in the [bypass] path, then perform step S1c in the [bychip] path, switch the second fluid distribution unit 302 to connect with a container containing another reagent or cleaning liquid, then execute step S1d and step S2d in the [bypass] path again, then perform step S1c and step S2c in the [bychip] path, and finally perform step 3c to discharge the waste liquid in the fluid drive module 40 into the waste liquid storage module 50.

[0193] 1 and 2 , the automatic cleaning process of the fluid transport system 100 includes cleaning the outer wall of the reagent needle 122, cleaning the inner wall of the reagent needle 122, cleaning the inner wall of the sample needle 112, cleaning the connecting pipes and components (such as valves) along the path, and draining the liquid in the flow channel.

[0194] 1) Cleaning process of the outer wall of the reagent needle 122:

[0195] Please refer to FIG13 , the specific method includes the following steps:

[0196] Step a: controlling the automatic cleaning module 60 so that the automatic cleaning module 60 is connected to the outer wall of the reagent needle 122 .

[0197] Specifically, one outlet of the third fluid dispensing unit 601 is connected to the outer wall of the reagent needle 122 through the pipeline 306 , and the main inlet of the third fluid dispensing unit 601 is connected to the cleaning liquid container 621 of the cleaning liquid storage unit 602 .

[0198] In step b, the automatic cleaning module 60 provides a driving force to load the cleaning liquid in the automatic cleaning module 60 onto the outer wall of the reagent needle 122 to clean the outer wall of the reagent needle 122 .

[0199] Specifically, the driving mechanism 614 is controlled to rotate forward, so that the cleaning liquid is transported from a cleaning liquid container 621 of the cleaning liquid storage unit 602 to the reagent needle 122 to flush the outer wall of the reagent needle 122 .

[0200] It can be understood that a multi-liquid collecting block 605 is provided on the pipeline 306 between the reagent storage unit 102 and the third fluid dispensing unit, which can clean the outer walls of multiple reagent needles 122.

[0201] 2) Cleaning process of the inner wall of the reagent needle 122:

[0202] Please refer to FIG14 and FIG2 together, the specific method includes the following steps:

[0203] Step a: controlling the automatic cleaning module 60 so that the automatic cleaning module 60 is in communication with the reagent needle 122 through the fluid dispensing module 30 .

[0204] Specifically, the third fluid dispensing unit 601 is controlled so that the cleaning liquid storage unit 602 is connected to the first fluid dispensing unit 301 through the second flow direction selection component 612, the driving mechanism 614, the first flow direction selection component 611, and the temporary storage pipeline 613, and then further connected to the reagent needle 122 through the second fluid dispensing unit 302.

[0205] In step b, the automatic cleaning module 60 provides a driving force so that the cleaning fluid in the automatic cleaning module 60 passes through the fluid dispensing module 30 and enters the reagent needle 122 to clean the inner wall of the reagent needle 122 and the connecting pipeline.

[0206] Specifically, the driving mechanism 614 is controlled to rotate forward, so that the cleaning liquid is temporarily stored in the temporary storage pipeline 613 by passing through the second flow direction selection component 612, the driving mechanism 614, and the first flow direction selection component 611 in sequence from the cleaning liquid storage unit 602. The first fluid distribution unit 301 is controlled to communicate with the second fluid distribution unit 302, and the second fluid distribution unit 302 is further controlled to communicate with the corresponding reagent needle 122. The driving mechanism 614 is controlled to rotate forward, so that the cleaning liquid in the temporary storage pipeline 613 enters the reagent needle 122 that needs to be cleaned. After cleaning the reagent needle 122, the waste liquid enters the reagent container 121, thereby cleaning the inner wall of the reagent needle 122 and the connecting pipeline. It is understandable that the reagent container 121 can also be connected to the waste liquid storage module 50 to further discharge the waste liquid into the waste liquid storage module 50.

[0207] It is also understandable that the inner walls and connecting pipes of different reagent needles 122 can be cleaned by switching different reagent needles 122, and the cleaning process of the reagent needle 122 using different cleaning liquids can be completed by switching different cleaning liquids.

[0208] 3) Cleaning process of connecting pipes and components (such as valves):

[0209] Please refer to FIG14 and FIG2 together, which includes the following steps:

[0210] Step a: controlling the automatic cleaning module 60 so that the automatic cleaning module 60 is in communication with the fluid driving module 40 .

[0211] Specifically, by controlling the third fluid distribution unit 601 , the cleaning liquid storage unit 602 is connected to the fluid driving module 40 through the second flow direction selection component 612 , the driving mechanism 614 , the first flow direction selection component 611 , and the temporary storage pipeline 613 .

[0212] In step b, the automatic cleaning module 60 or the fluid driving module 40 provides driving force, so that the cleaning fluid in the automatic cleaning module 60 enters the fluid driving module 40 through the temporary storage pipeline 613 to clean the connecting pipelines and components on the path.

[0213] Specifically, the drive mechanism 614 is controlled to rotate forward, causing the cleaning fluid to flow from the cleaning fluid storage unit 602 through the second flow direction selection component 612, the drive mechanism 614, and the first flow direction selection component 611, and enter the temporary storage pipeline 613 for temporary storage. The first fluid distribution unit 301 is then controlled to communicate with the second fluid distribution unit 302, and the tapping port 322 of the second fluid distribution unit 302 is adjusted to a blocked position, allowing the fluid drive module 40 to communicate with the waste liquid storage module 50. Thereafter, the drive mechanism 614 can be controlled to rotate forward, causing the cleaning fluid in the temporary storage pipeline 613 to enter the fluid drive module 40 and finally be discharged into the waste liquid storage module 50, thereby completing the cleaning of the connecting pipelines, valves, and other components.

[0214] It is understandable that the cleaning of connecting pipes and valves and other components can also be completed by the following steps:

[0215] In the first step, by controlling the third fluid distribution unit 601, the cleaning liquid storage unit 602 is connected to the fluid driving module 40 through the second flow direction selection component 612, the first flow direction selection component 611 and the temporary storage pipeline 613. At this time, the branch interface 322 of the second fluid distribution unit 302 is switched to a blocked position.

[0216] In the second step, the fluid driving module 40 provides driving force to extract the cleaning fluid from the cleaning fluid storage unit 602 and sequentially pass through the temporary storage pipeline 613 into the fluid driving module 40 .

[0217] The third step is to connect the fluid driving module 40 with the waste liquid storage module 50, discharge the waste liquid temporarily stored in the fluid driving module 40 into the waste liquid storage module 50, and complete the cleaning of the connecting pipes and valves and other components.

[0218] 4) Cleaning the inner wall of the sample needle 112 and the connecting pipe:

[0219] Please refer to FIG. 14 and FIG. 15 together with FIG. 2 , which includes the following steps:

[0220] Step a: controlling the automatic cleaning module 60 so that the automatic cleaning module 60 is in communication with the fluid driving module 40 .

[0221] Specifically, by controlling the third fluid distribution unit 601 , the cleaning liquid storage unit 602 is connected to the fluid driving module 40 through the second flow direction selection component 612 , the driving mechanism 614 , the first flow direction selection component 611 , and the temporary storage pipeline 613 .

[0222] Step b: providing driving force through the fluid driving module 40 or the automatic cleaning module 60 so that the cleaning fluid in the automatic cleaning module 60 enters the fluid driving module 40 for temporary storage.

[0223] Method 1: As shown in FIG14 , and in conjunction with FIG2 , the drive mechanism 614 is controlled to rotate forward, causing the cleaning liquid to flow from the cleaning liquid storage unit 602 through the second flow direction selection component 612, the drive mechanism 614, and the first flow direction selection component 611, and then enter the temporary storage pipeline 613 for temporary storage. The shut-off valve DT6 is then opened, allowing the cleaning liquid in the temporary storage pipeline 613 to enter the channel of the drive assembly 402.

[0224] The second method: As shown in Figure 15 and in combination with Figure 2, open the stop valve DT6, and directly use the fluid drive module 40 to provide driving force, so that the cleaning liquid passes through the second flow direction selection component 612, the first flow direction selection component 611, and the temporary storage pipeline 613 from the cleaning liquid storage unit 602 into the channel of the drive component 402.

[0225] Step c: controlling the fluid dispensing module 30 to connect the fluid driving module 40 to the sample needle 112 .

[0226] The stop valve DT6 is closed, and the first fluid dispensing unit 301 is controlled to be in the second state, so that the driving assembly 402 is connected to the sample needle 112 .

[0227] In step d, the fluid driving module 40 provides driving force to allow the cleaning fluid temporarily stored in the fluid driving module 40 to enter the sample needle 112 through the fluid distributing module 30 to clean the inner wall of the sample needle 112 and the connecting pipe.

[0228] The drive assembly 402 moves upward, and the cleaning fluid temporarily stored in the channel of the drive assembly 402 enters the reagent needle 122 through the first fluid distribution unit 301, cleaning the reagent needle 122, the first fluid distribution unit 301 and the fluid pipeline. The waste liquid after cleaning enters the sample container 111, and finally the waste liquid is cleaned.

[0229] 5) The process of discharging the liquid in the pipeline includes:

[0230] As shown in FIG. 16 , by controlling the automatic cleaning module 60 , the gas port 623 of the cleaning liquid selection unit 603 is connected to the pipeline of the fluid transportation system 100 , thereby emptying the pipeline.

[0231] The following is a detailed description of the automatic cleaning process:

[0232] Step a: one of the inlets in the two-way solenoid valve group DT10-14 is opened, and the required cleaning reagent is selected. If the selected cleaning reagent corresponds to air, it is used to empty the fluid in the pipeline, as shown in FIG16 .

[0233] In step b, the three-way solenoid valves DT7 and DT8 are de-energized, and the diaphragm pump flows forward to the second fluid dispensing unit 302 at a certain speed. The required cleaning reagent in step a is extracted and transported to the outer wall of the reagent needle 122, so as to clean the outer wall of the reagent needle 122, as shown in Figure 13.

[0234] In step c, the three-way solenoid valve DT7 is energized and DT8 is de-energized. The diaphragm pump rotates at a certain speed toward the second fluid distribution unit 302. The required cleaning liquid in step a is extracted and output from the three-way solenoid valve DT7 to the stop valve DT6 connecting pipeline at a certain flow rate. The fluid path is shown in Figure 13.

[0235] In step d, the stop valve DT6 is opened, the first fluid dispensing unit 301 is switched to the third state position, and the second fluid dispensing unit 302 is switched to the designated branch interface 322. The cleaning liquid output in step c passes through the stop valve DT6, the first fluid dispensing unit 301, the second fluid dispensing unit 302, the reagent needle 122 connected to the designated branch interface 322, and finally enters the reagent container 121, completing the cleaning of the inner wall of the reagent needle 122 and the connecting pipeline.

[0236] In step e, according to step d, the second fluid dispensing unit 302 is switched to the branch interface 322 connected to different reagent needles 122 to complete the cleaning of the inner walls and connecting pipes of the different reagent needles 122.

[0237] Step f, according to step e, switch another inlet of the two-way solenoid valve group DT10-14 in step a, and select different cleaning fluids for multiple cleanings.

[0238] In step g, the two-way solenoid valve assembly can also be connected to the waste liquid storage module 50. Specifically, DT14 of the two-way solenoid valve assembly DT10-14 is connected to the waste liquid storage module 50. According to step d, the diaphragm pump reverses at a certain speed, DT14 of the two-way solenoid valve assembly DT10-14 opens, and the waste liquid in the reagent container 121 is extracted and discharged into the waste liquid storage module 50, as shown in Figure 12.

[0239] In step h, the shut-off valve DT6 is opened, the first fluid distribution unit 301 is switched to the third state position, the second fluid distribution unit 302 is switched to a designated blocked branch port 322, and the reversing assembly 403 of the fluid drive device 401 is switched to open the second drive port 432 and the third drive port 433 at the same time. The cleaning fluid output in step c passes through the reversing assembly 403 and is finally discharged to the waste liquid storage module 50, completing the cleaning of the pipes and valves along the path.

[0240] In step i, the three-way solenoid valve DT7 loses power and DT8 gains power, the diaphragm pump stops working, and the cleaning liquid required in step a is directly connected to the stop valve DT6 connection pipeline, and the fluid path is shown in FIG15 .

[0241] In step j, the stop valve DT6 is opened, the first fluid distribution unit 301 is switched to the third state position, the second fluid distribution unit 302 is switched to a designated blocked branch interface 322, the reversing component 403 is switched to the second drive interface 432, and the drive component 402 moves downward to extract the required cleaning fluid connected to the stop valve DT6 in step i. The cleaning fluid output from the stop valve DT6 passes through the stop valve DT6 and the 1-to-4 liquid collection block in sequence, and finally enters the channel of the drive component 402, completing the cleaning of the pipes and valves on the path.

[0242] In step k, the reversing component 403 switches to the third driving interface 433 , and the driving component 402 moves upward. In step j, the waste liquid in the driving component 402 is discharged into the waste liquid storage module 50 .

[0243] In step 1, the shut-off valve DT6 is opened, the first rotor 316 of the first fluid distribution unit 301 rotates to the third state position, the second fluid distribution unit 302 switches to a designated blocked tap port 322, the reversing assembly 403 switches to the second drive port 432, and the drive assembly 402 moves downward to extract the required cleaning fluid connected to the shut-off valve DT6 in step i. The cleaning fluid passes through the 1-to-4 splitting block and the second drive port 432 of the reversing assembly 403 in sequence, and enters the channel of the drive assembly 402 for temporary storage.

[0244] In step m, the first fluid dispensing unit 301 is switched to the second state position, the reversing component 403 is switched to the first drive interface 431, and the drive component 402 moves upward. The cleaning fluid temporarily stored in the drive component 402 in step j or step l is pushed out, passes through the first drive interface 431 of the reversing component 403, the first fluid dispensing unit 301, and finally passes through the sample needle 112 and is discharged into the sample container 111, completing the cleaning of the first fluid dispensing unit 301, the inner wall of the sample needle 112, and the pipeline on the path.

[0245] The fluid transport system 100 provided in the embodiment of the present application integrates a fluid storage module 10, a fluid use module 20, a fluid distribution module 30 and a fluid drive module 40 in a biochemical substance analysis device. Through the mutual cooperation of the above structures, the function of independently loading the same or different types of samples through the branch channel of the biochemical substance analysis device can be realized. The operation is simple and automatic loading can be achieved. In the process of independently loading samples through the branch channel, there is no need to use any device outside the device, which simplifies the structural complexity of the device, reduces costs, and further reduces the operational difficulty of independently loading samples through the branch channel.

[0246] Moreover, through the mutual cooperation of the above structures, the fully automatic cleaning function of the fluid transport system 100 (including cleaning, emptying, drying and other processes) can be realized. Different cleaning liquids can be automatically replaced to clean different flow paths and components. The cleaning process is simple and does not require any manual intervention, which improves convenience.

[0247] In addition, the fluid transport system 100 can be adapted to existing biochemical substance analysis equipment to achieve automatic transport of different biochemical reaction reagents.

[0248] Please refer to FIG. 17 . Based on the same inventive concept, an embodiment of the present application further provides a biochemical substance analysis device 1000 . The biochemical substance analysis device 1000 includes the fluid transport system 100 as described above.

[0249] Specifically, the biochemical substance analysis device 1000 can be a gene sequencer, further comprising an imaging detection module 200. A fluid utilization module (e.g., a sequencing chip) that completes a biochemical reaction in the fluid transport system 100 can be further transferred to the imaging detection module 200 for imaging detection and analysis, thereby completing the gene sequencing process. The transfer of the fluid utilization module can be accomplished by a transfer mechanism such as a manipulator or a movable track.

[0250] In some embodiments, the biochemical substance analysis device 1000 further includes a housing 300 , and the waste liquid storage module in the fluid transport system 100 may be located inside the housing 300 or outside the housing 300 .

[0251] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application and are not intended to limit the present application. Although the present application has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present application may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present application.

Claims

1. A fluid transportation system, characterized in that, Comprising: A fluid storage module, a fluid usage module, a fluid distribution module, and a fluid driving module. The fluid storage module is used for storing fluid; the fluid usage module includes multiple independent flow channels, and the flow channels are used to complete biochemical reactions. Among them, the liquid inlet end of each flow channel is communicated with the fluid storage module through the fluid distribution module, and the liquid outlet end of each flow channel is communicated with the fluid driving module; the fluid distribution module is used for selecting the transportation direction of the fluid; the fluid driving module is used for generating a driving force to enable the fluid located in the fluid storage module to be independently loaded into each flow channel through the fluid distribution module.

2. The fluid transportation system according to claim 1, characterized in that, The fluid storage module includes a sample storage unit and a reagent storage unit. The fluid distribution module includes a first fluid distribution unit and a second fluid distribution unit that are communicated with each other. The sample storage unit is communicated with the flow channel through the first fluid distribution unit. The reagent storage unit is communicated with the flow channel through the second fluid distribution unit and the first fluid distribution unit. Both the first fluid distribution unit and the second fluid distribution unit are used for controlling the opening or closing of the fluid pipeline according to a preset time sequence to select the transportation direction of the fluid.

3. The fluid transportation system according to claim 2, characterized in that, The sample storage unit includes multiple sample containers. Each flow channel is communicated with the corresponding sample container through the first fluid distribution unit. The driving force is used for enabling the biological sample located in the sample container to be independently loaded into the corresponding flow channel through the first fluid distribution unit.

4. The fluid transportation system according to claim 3, characterized in that, The second fluid distribution unit is also communicated with a designated sample container. The driving force is also used for enabling the biological sample located in the designated sample container to be loaded into each flow channel in sequence through the second fluid distribution unit and the first fluid distribution unit.

5. The fluid transportation system according to claim 2, characterized in that, The first fluid distribution unit is also communicated with the fluid driving module. The driving force is also used for enabling the reagent located in the reagent storage unit to be loaded into the fluid driving module in sequence through the second fluid distribution unit and the first fluid distribution unit.

6. The fluid transportation system according to claim 5, characterized in that, The first fluid distribution unit includes a first stator and a first rotor arranged in a stacked manner. The first stator is provided with a first interface communicated with each flow channel, a second interface communicated with the driving module, a third interface communicated with the sample storage unit, and a fourth interface communicated with the second fluid distribution unit. The first rotor is provided with multiple first communication ports, a second communication port, and a third communication port. Among them, during the rotation process of the first rotor, it is used for changing the relative position between the first rotor and the first stator, so that the first interface is communicated with the third interface through the first communication port; or, enabling the fourth interface to be communicated with all the First interfaces through the second communication port; or, enabling the second interface to be communicated with the fourth interface through the third communication port.

7. The fluid transportation system according to claim 6, characterized in that, The second fluid distribution unit includes a second stator and a second rotor. The second stator is provided with a main interface communicating with the fourth interface and a sub-interface communicating with the reagent storage unit. The second rotor is provided with a fourth communication port for communicating the main interface with the sub-interface. During the rotation of the second rotor, it is used to change the relative position between the second rotor and the second stator, so that the main interface communicates with the corresponding sub-interface through the fourth communication port.

8. The fluid transportation system according to claim 1, characterized in that, The fluid driving module includes a plurality of driving components and a commutation component provided on each driving component. Each commutation component is used to communicate with one of the flow channels, and the driving component is used to provide the driving force.

9. The fluid transportation system according to claim 1, characterized in that, The fluid transportation system further includes an automatic cleaning module, which is used to clean the fluid transportation system.

10. The fluid transportation system according to claim 9, characterized in that, The fluid storage module includes a reagent storage unit, and the reagent storage unit includes a reagent needle. The automatic cleaning module communicates with the outer wall of the reagent needle, and the automatic cleaning module is also used to provide a driving force to transport the cleaning liquid in the automatic cleaning module to the outer wall of the reagent needle to clean the outer wall of the reagent needle.

11. The fluid transportation system according to claim 9, characterized in that, The fluid storage module includes a reagent storage unit, and the reagent storage unit includes a reagent needle. The automatic cleaning module communicates with the reagent needle through the fluid distribution module, and the automatic cleaning module is also used to provide a driving force to enable the cleaning liquid in the automatic cleaning module to enter the reagent needle through the fluid distribution module to clean the inner wall of the reagent needle and the connecting pipeline.

12. The fluid transportation system according to claim 9, characterized in that, The fluid storage module includes a sample storage unit, and the sample storage unit includes a sample needle; The automatic cleaning module communicates with the fluid driving module, and the fluid driving module or the automatic cleaning module is used to provide a driving force to enable the cleaning liquid in the automatic cleaning module to enter the fluid driving module; The fluid driving module also communicates with the sample needle through the fluid distribution module, and the fluid driving module is also used to provide a driving force to enable the cleaning liquid in the fluid driving module to enter the sample needle through the fluid distribution module to clean the inner wall of the sample needle and the connecting pipeline.

13. The fluid transportation system according to any one of claims 9 to 12, characterized in that, The automatic cleaning module includes a third fluid distribution unit and a cleaning liquid storage unit that are interconnected; The third fluid distribution unit includes a first flow direction selection component, a second flow direction selection component, a driving mechanism, and a temporary storage pipeline. The first end of the temporary storage pipeline is connected to the first outlet of the first flow direction selection component and the first outlet of the second flow direction selection component. The second end of the temporary storage pipeline is connected to communicate with the fluid distribution module and the fluid driving module. The second outlet of the first flow direction selection component is connected to the reagent storage unit. The common inlet of the second flow direction selection component is connected to the cleaning liquid storage unit. The two ends of the driving mechanism are respectively connected to the common inlet of the first flow direction selection component and the second outlet of the second flow direction selection component. The driving mechanism is used to provide a driving force and is also used to change the direction of fluid transportation.

14. The fluid transportation system according to claim 13, characterized in that, A cleaning liquid selection unit is provided between the third fluid distribution unit and the cleaning liquid storage unit. The cleaning liquid selection unit includes a plurality of inlets and a common outlet. The common outlet is connected to the total inlet of the third fluid distribution unit, and the plurality of inlets communicate with the cleaning liquid storage unit.

15. The fluid transportation system according to claim 14, characterized in that, One inlet of the cleaning liquid selection unit is a gas port for communicating with gas.

16. The fluid transportation system according to claim 1, characterized in that, The fluid transportation system further includes a waste liquid storage module, which communicates with the fluid driving module and the fluid distribution module respectively.

17. A fluid transportation system, characterized in that,Comprising: A fluid storage module, a fluid usage module, a fluid driving module, and an automatic cleaning module, The fluid storage module is used for storing fluid; The fluid usage module includes a flow channel for completing a biochemical reaction. The inlet end of the flow channel communicates with the fluid storage module, and the outlet end of the flow channel communicates with the fluid driving module; The fluid driving module is used for generating a driving force to load the fluid located in the fluid storage module into the flow channel; The automatic cleaning module is used for cleaning the fluid transportation system.

18. The fluid transportation system according to claim 17, wherein, The fluid storage module includes a reagent storage unit, and the reagent storage unit includes a reagent needle. The automatic cleaning module communicates with the outer wall of the reagent needle, and the automatic cleaning module is further used for providing a driving force to transport the cleaning liquid located in the automatic cleaning module to the outer wall of the reagent needle to clean the outer wall of the reagent needle.

19. The fluid transportation system according to claim 17, wherein, The fluid storage module includes a reagent storage unit, and the reagent storage unit includes a reagent needle. The automatic cleaning module communicates with the fluid driving module. The automatic cleaning module is used for providing a driving force to make the cleaning liquid located in the automatic cleaning module enter the fluid driving module. The fluid driving module also communicates with the reagent needle, and the fluid driving module is further used for providing a driving force to make the cleaning liquid located in the fluid driving module enter the reagent needle to clean the inner wall of the reagent needle and the connecting pipeline.

20. The fluid transportation system according to claim 17, wherein, The fluid storage module includes a sample storage unit, and the sample storage unit includes a sample needle; the automatic cleaning module communicates with the fluid driving module. The automatic cleaning module or the fluid driving module is used for providing a driving force to make the cleaning liquid located in the automatic cleaning module enter the fluid driving module. The fluid driving module also communicates with the sample needle, and the fluid driving module is further used for providing a driving force to make the cleaning liquid located in the fluid driving module enter the sample needle to clean the inner wall of the sample needle and the connecting pipeline.

21. The fluid transportation system according to claim 20, wherein, The automatic cleaning module includes a third fluid distribution unit and a cleaning liquid storage unit that communicate with each other, The third fluid distribution unit includes a first flow direction selection component, a second flow direction selection component, a driving mechanism, and a temporary storage pipeline. The first end of the temporary storage pipeline is connected to the first outlet of the first flow direction selection component and the first outlet of the second flow direction selection component. The second end of the temporary storage pipeline is connected to the fluid storage module and the fluid driving module in communication. The second outlet of the first flow direction selection component is connected to the fluid storage module. The common inlet of the second flow direction selection component is connected to the cleaning liquid storage unit. Both ends of the driving mechanism are respectively connected to the common inlet of the first flow direction selection component and the second outlet of the second flow direction selection component. The driving mechanism is used to provide a driving force, and the driving mechanism is also used to change the direction of fluid transportation.

22. The fluid transportation system according to claim 21, wherein, A cleaning liquid selection unit is provided between the third fluid distribution unit and the cleaning liquid storage unit. The cleaning liquid selection unit includes a plurality of inlets and a common outlet. The common outlet is connected to the total inlet of the third fluid distribution unit. The plurality of inlets are in communication with the cleaning liquid storage unit.

23. The fluid transportation system according to claim 22, wherein, One inlet of the cleaning liquid selection unit is a gas port for communicating with gas.

24. The fluid transportation system according to claim 17, wherein, The fluid use module includes a plurality of the flow channels. The fluid transportation system further includes a fluid distribution module. The fluid distribution module is respectively in communication with the flow channels and the fluid storage module. The fluid driving module is used to provide a driving force so that the fluid located in the fluid storage module passes through the fluid distribution module and is independently loaded into each of the flow channels.

25. The fluid transportation system according to claim 24, wherein, The fluid storage module includes a sample storage unit and a reagent storage unit. The fluid distribution module includes a first fluid distribution unit and a second fluid distribution unit that are in communication with each other. The sample storage unit is in communication with the flow channels through the first fluid distribution unit. The reagent storage unit is in communication with the flow channels through the second fluid distribution unit and the first fluid distribution unit. Both the first fluid distribution unit and the second fluid distribution unit are used to control the opening or closing of the fluid pipeline according to a preset time sequence to select the fluid transportation direction.

26. The fluid transportation system according to claim 25, wherein, The sample storage unit includes a plurality of sample containers. Each of the flow channels is in communication with a corresponding sample container through the first fluid distribution unit. The driving force is used to independently load the biological sample located in the sample container into the corresponding flow channel through the first fluid distribution unit.

27. The fluid transportation system according to claim 26, wherein, The second fluid distribution unit is also in communication with a designated sample container. The driving force is also used to load the biological sample located in the designated sample container into each of the flow channels sequentially through the second fluid distribution unit and the first fluid distribution unit.

28. The fluid transportation system according to claim 25, wherein, The first fluid distribution unit is also in communication with the fluid driving module. The driving force is also used to load the reagent located in the reagent storage unit into the fluid driving module sequentially through the second fluid distribution unit and the first fluid distribution unit.

29. A fluid transportation method, wherein, Including: Control the fluid distribution module to connect the flow channels in the fluid usage module with the fluid storage module, where the fluid usage module includes a plurality of independent flow channels, and each flow channel is connected to the fluid storage module; and Provide a driving force through the fluid driving module so that the fluid located in the fluid storage module is independently loaded into each flow channel via the fluid distribution module.

30. The fluid transportation method according to claim 29, wherein, The fluid storage module includes a sample storage unit, and the sample storage unit includes a plurality of sample containers, The fluid transportation method includes: Control the fluid distribution module to connect each flow channel with one of the sample containers; and Provide a driving force for each flow channel through the fluid driving module so that the biological sample located in the sample container is independently loaded into the corresponding flow channel via the fluid distribution module.

31. The fluid transportation method according to claim 30, wherein, The fluid transportation method further includes: Control the fluid distribution module to connect all the flow channels with one specified sample container; and Provide a driving force through the fluid driving module so that the biological sample located in the specified sample container is independently or simultaneously loaded into each flow channel via the fluid distribution module.

32. The fluid transportation method according to claim 29, characterized in that, The fluid storage module includes a reagent storage unit connected to the fluid distribution module, The fluid transportation method includes: Control the fluid distribution module to connect the flow channel with the reagent storage unit; and Provide a driving force through the fluid driving module so that the reagent located in the reagent storage unit is loaded into the flow channel via the fluid distribution module.

33. The fluid transportation method according to claim 32, characterized in that, The fluid transportation method further includes: Control the fluid distribution module to connect the fluid driving module with the reagent storage unit through the fluid distribution module; and Provide a driving force through the fluid driving module so that the reagent located in the reagent storage unit is at least loaded into the fluid pipeline between the fluid distribution module and the fluid driving module.

34. The fluid transportation method according to claim 29, characterized in that, The fluid transportation method further includes: automatically cleaning the fluid transportation system.

35. The fluid transportation method according to claim 34, characterized in that, The fluid storage module includes a reagent storage unit, and the reagent storage unit includes a reagent needle, The automatically cleaning the fluid transportation system includes: cleaning the outer wall of the reagent needle, including: Control the automatic cleaning module to connect the automatic cleaning module to the outer wall of the reagent needle; and Provide a driving force through the automatic cleaning module so that the cleaning liquid located in the automatic cleaning module is transported to the outer wall of the reagent needle to clean the outer wall of the reagent needle.

36. The fluid transportation method according to claim 35, characterized in that, The automatically cleaning the fluid transportation system further includes: cleaning the inner wall of the reagent needle and the connecting pipeline, including the steps of: Control the automatic cleaning module to connect the automatic cleaning module with the reagent needle through the fluid distribution module; and Provide a driving force through the automatic cleaning module so that the cleaning liquid located in the automatic cleaning storage module enters the reagent needle through the fluid distribution module to clean the inner wall of the reagent needle and the connecting pipeline.

37. The fluid transportation method according to claim 34, characterized in that, The automatically cleaning the fluid transportation system includes: cleaning the connecting pipeline and the components on the path, including: Control the automatic cleaning module to connect the automatic cleaning module with the fluid driving module; and Provide a driving force through the automatic cleaning module or the fluid driving module to cause the cleaning liquid in the automatic cleaning module to enter the fluid driving module, so as to clean the connecting pipeline and the components on the path.

38. The fluid transportation method according to claim 34, characterized in that, The fluid storage module includes a sample storage unit, and the sample storage unit includes a sample needle. Automatically cleaning the fluid transportation system includes: cleaning the inner wall of the sample needle and the connecting pipeline, including: Control the automatic cleaning module to connect the automatic cleaning module with the fluid driving mo dule; Provide a driving force through the fluid driving module or the automatic cleaning module to cause the cleaning liquid in the automatic cleaning module to enter the fluid driving module for temporary storage; Control the fluid distribution module to connect the fluid driving module with the sample needle; and Provide a driving force through the fluid driving module to cause the cleaning liquid temporarily stored in the fluid driving module to enter the sample needle through the fluid distribution module, so as to clean the inner wall of the sample needle and the connecting pipeline.

39. The fluid transportation method according to any one of claims 29 to 38, characterized in that,The fluid transportation method further includes: Provide a driving force through the fluid driving module to pump the waste liquid in the pipeline into the fluid driving module; Control the fluid driving module to connect with the waste liquid storage module; and Provide a driving force through the fluid driving module to discharge the waste liquid in the fluid driving module into the waste liquid storage module.

40. A biochemical substance analysis device, characterized in that, It includes the fluid transportation system according to any one of claims 1 to 28.

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