Method for enhanced extraction of functional active ingredients from berries by using continuous high-pulse electric field treatment

Abstract

A method for enhanced extraction of functional active ingredients from berries by using continuous high-pulse electric field treatment. The method comprises the following steps: after a treatment chamber apparatus is cooled to a set temperature by means of a cooling apparatus, setting high-pulse power source output parameters; a high-pulse power source generating bipolar symmetric square waves; setting pulse electric field parameters, the distance between two titanium alloy electrodes being 100 mm; conveying pretreated berry materials to the body of a treatment chamber; and performing high-pulse electric field treatment on the berry materials, so as to obtain a berry extraction solution containing active ingredients. In the present invention, a continuous circular-to-square corrosion-resistant pulse electric field treatment chamber is used, such that the overall structure is convenient for assembly and machining; additional treatment chambers can be added according to actual requirements; and synchronous cooling can also be realized, thereby realizing low-temperature extraction and activity preservation of functional active ingredients while protecting electrodes.

Description

A method for enhanced extraction of functional active ingredients from berries using continuous high-voltage pulsed electric field treatment Technical Field

[0001] The present invention relates to the fields of food mechanical processing and food process optimization application, and in particular to a method for enhanced extraction of functional active ingredients of berries by continuous high-voltage pulse electric field treatment. Background Art

[0002] High-voltage pulsed electric fields (HPEF) technology works by applying a specific pulsed high voltage between two electrodes, leveraging the high potential difference between them to generate a high-intensity electric field (for example, using a parallel plate electric field: field strength E = U / d, where E is measured in kV / cm; U is the high voltage in kV; and d is the distance between the parallel plate electrodes in cm). When the treated material is subjected to a high-voltage electric field of a certain intensity and direction, the inherent asymmetry of the molecular components of the material leads to a polarized arrangement between tissues, creating a potential difference across the cell membranes of some tissues (which serves to balance or offset the externally applied electric field pressure). When the applied electric field intensity exceeds the tolerance of the cell membrane, charged particles in the cytoplasm are pulled by the electric field, creating an internal electric field that squeezes the cell membrane. This increases the permeability of the cell membrane, weakens the membrane strength, and disrupts the tissue structure, causing cytoplasm to escape from the cell membrane, thereby increasing the content of certain components in the material. High-voltage pulses have strong instantaneous destructive power, low average power, and insignificant heat generation. They can be quickly and efficiently used to extract functional active ingredients, such as polyphenols, flavonoids, and other functional ingredients from processing by-products such as grape skins and pomace. They will not affect the structure of the extracts or the flavor of the materials. They have a wide range of applications and have been favored by many research fields.

[0003] However, due to the complexity of food material properties, the characteristics of different food materials and processing methods vary greatly. The relevant processing models and product development and operation process regulations are still not perfect. In addition, due to the huge scale of food processing in the field, many materials to be processed are mostly slurry materials. The resistance of slurry materials is not fixed, and the conductivity is generally high. Large-scale processing is carried out through pumping and other methods. The conductivity varies greatly under the action of high-voltage instantaneous electric fields. Its pulsed electric field discharge treatment model is different from the traditional pure resistive discharge system. The material processing process involves resistive-capacitive discharge, which further increases the difficulty of material processing. At the same time, continuous pulsed electric field treatment places higher requirements on the stability and reliability of the power supply, the uniformity of material processing in the pulsed electric field treatment room, and the corrosion resistance of the electrodes.

[0004] The current high-voltage pulsed electric field equipment is mainly laboratory-scale equipment. Chinese Patent 202022239622.3 discloses a high-voltage pulsed electric field sterilization device, which discloses a pulsed electric field sterilization device with multiple needle-shaped electrodes and a liftable treatment chamber. By placing the treatment sample on the lifting device and adjusting the height by lifting, it is used for sterilization treatment of materials such as meat; Chinese Patent 202120190221.5 discloses an ultrasonic-assisted high-voltage pulsed electric field extraction tank, which adds a static high-voltage pulsed electric field to the top of the cylinder, installs ultrasonic waves at the bottom, and builds related equipment; Existing patent CN108452006A discloses a method for extracting plant polyphenols by combining pulsed electric field-two-phase aqueous phase-column chromatography, using a pulsed electric field static treatment chamber , perform 20-30 pulse discharge treatments to realize the extraction of active ingredients of plants by pulse electric field; Chinese patent 202122150584.9 discloses an ultrasonic-assisted high-voltage pulse electric field extraction equipment for bergamot effective ingredients, and an ultrasonic-assisted pulse equipment is used to enhance the extraction of bergamot effective ingredients; Chinese patent 201710929691.7 discloses an apparatus and method for extracting medicinal components of traditional Chinese medicine using electroporation technology. The processing chamber is a coaxial processing chamber containing a high-voltage electrode array and a grounded electrode array. The electrode distance is controlled between 0.5 and 5 cm, which can meet the extraction and processing of small particles or liquid materials.

[0005] In summary, the existing high-voltage pulse electric field extraction equipment has the following problems:

[0006] 1. Most of the current high-voltage pulsed electric field extraction equipment is laboratory-scale equipment or static processing, which is small in scale. In addition, most extraction methods use ultrasonic auxiliary processing, with ultrasonic action as the main factor. There is a lack of industrial processing equipment for slurry materials, which cannot meet the needs of industrial extraction and processing.

[0007] 2. The current continuous high-voltage pulse electric field extraction equipment is mainly of common field and coaxial types. Flat-plate processing devices are relatively rare due to their complexity. They all ignore the temperature rise control and electrode corrosion generated by the energy during the high-voltage pulse electric field treatment process. The higher the temperature, the faster the electrode corrosion rate. In severe cases, the electrode dissolves the contaminated material and the processing safety cannot be guaranteed.

[0008] 3. The existing high-voltage pulse electric field treatment material temperature rise control adopts pre-cooling of the material or cooling after treatment. The material is rapidly heated at both ends of the electrode and then condensed through a long pipeline. The active ingredients are not effectively protected from destruction and loss during the process. Summary of the Invention

[0009] In order to overcome the above-mentioned problems of existing equipment and production methods, the present invention provides a method for enhanced extraction of functional active ingredients in berries using continuous high-voltage pulsed electric field treatment. The method adopts a high-voltage pulse square wave treatment power supply as the treatment power supply (power supply parameters: 0-±25 kV, symmetrical pulse square wave, 1-1000 Hz adjustable, 1-20 µs adjustable, 1000 A protection current), is equipped with a peristaltic pump to transport materials and a synchronous cooling treatment chamber system. The cooling system cools the materials on the one hand and provides condensation protection for the treatment chamber electrodes on the other hand, thereby solving the risk of high-temperature electrode corrosion, greatly reducing electrode corrosion, increasing electrode life, reducing the risk of electrode dissolution of contaminated materials, and improving the safety of processed materials. The method has a maximum processing capacity of 3.0 t / h, which can meet the industrial processing requirements of a variety of pumpable materials.

[0010] The purpose of the present invention is to provide a method for enhanced extraction of functional active ingredients of berries treated with a continuous high-voltage pulse electric field, which is achieved by connecting an extraction device and a material control system through a high-voltage pulse electric field. The high-voltage pulse electric field extraction device and the material control system include a processing chamber device and a cooling device. The processing chamber device includes a titanium alloy electrode, a stainless steel sealing plate arranged on both sides and an insulating bracket arranged in the middle. The titanium alloy electrode is symmetrically embedded in the insulating bracket, and the stainless steel sealing plate is fixed to the outer edge of the insulating bracket. The stainless steel sealing plate, the titanium alloy electrode and the insulating bracket form a sandwich cavity as a whole. The bottom of the sandwich cavity is externally connected to the refrigerant inlet and the refrigerant outlet of the cooling device. The refrigerant enters the sandwich cavity through the refrigerant inlet to cool the titanium alloy electrode, and then the refrigerant is discharged through the refrigerant outlet. The output end of the high-voltage pulse power supply is connected to the titanium alloy electrode to generate a high-voltage pulse electric field; the method comprises the following steps: after the processing chamber device is cooled by the cooling device to reach a set temperature, the output parameters of the high-voltage pulse power supply are set, the high-voltage pulse power supply generates a bipolar symmetrical square wave, the pulse electric field parameters are set, and the distance between the two titanium alloy electrodes is 100 mm, transporting the pretreated berry material to a processing chamber device, subjecting the berry material to high-voltage pulse electric field treatment, with the berry material feed temperature and discharge temperature being the same, to obtain a berry extract containing active ingredients.

[0011] The technical principle of the method proposed in the present invention is: when berry materials pass through a treatment chamber loaded with a high-voltage pulse electric field, an internal electric field will quickly form inside the material to cope with the external electric field pressure. Since the applied external pulse high voltage is a symmetrical bipolar pulse, the polarity of the external pulse voltage changes. For example, when the loaded voltage is a reverse voltage, the instantaneous potential difference applied to the berry quickly changes to double the voltage intensity. When the intensity exceeds the intensity that the berry cells can withstand, the berry cells quickly rupture, realizing the dissolution of functional components in the cells; since the material processing time is short (the total processing time is less than 5 ms), the heat generated by the high voltage instantaneously is small, and since the cavity of the processing chamber is synchronously cooled by the circulation of coolant, the temperature rise is reduced, thereby realizing the protection of heat-sensitive components. By pre-cooling the electrode, the temperature difference and heat transfer efficiency between the cooling electrode and the material are improved, and the flow rate of the material conveying pump is controlled in coordination to ensure that the material before and after the cavity treatment remains in a non-heating state, that is, the temperature of the material in and out of the cavity remains unchanged. The high-voltage pulse electric field causes the internal rupture of the material in a short period of time, the polar molecules are oriented and moved in a directed manner, and the thermal effect caused by a small part of the high-voltage pulse current is completely balanced by the cooling system. The high-voltage pulse electric field electrode material is made of Ti-0.2Pd corrosion-resistant and high-hardness titanium alloy. The bipolar electrode is cooled by coolant to always keep the electrode at 0°C or below. At low temperatures, titanium alloy has strong corrosion resistance, and a thin layer of ice is formed on the surface of the titanium alloy electrode at low temperatures. The ice layer buries the titanium alloy electrode, isolating the titanium electrode from direct contact with the load material, avoiding electrode corrosion, and improving the protection of the titanium alloy electrode. In the material processing process, the processing pulse width is always ≤10 µs, while controlling the frequency within the range of 200-500Hz with a narrow pulse width, the energy density acting on the titanium alloy electrode per unit time is reduced, the titanium alloy electrode cannot meet the conditions for electron escape, and the electrode corrosion is reduced; the titanium alloy electrode is a smooth parallel plate electrode, and its edge is embedded in the insulating material polytetrafluoroethylene, which avoids edge high-voltage discharge. The parallel plate electrode has a uniform electric field distribution, which reduces the local breakdown effect of high-conductivity materials under high voltage in the processing chamber, reduces the current effect, and correspondingly improves the electric field effect. The strengthening of the electric field effect correspondingly expands the high-voltage electric field-induced material tissue rupture effect; at the same time, the bipolar symmetrical square wave is used to restrain the flow of bidirectional local current-carrying electrons on the electrode, reducing the overflow of high-energy active electrons and achieving pollution-free slurry treatment.

[0012] Preferably, the titanium alloy electrode includes a first titanium alloy electrode and a second titanium alloy electrode symmetrically embedded in the insulating bracket. The titanium alloy electrode material adopts Ti-0.2Pd corrosion-resistant and high-hardness titanium alloy. The stainless steel sealing plate includes a first stainless steel sealing plate and a second stainless steel sealing plate symmetrically arranged on both sides of the processing chamber device. The first stainless steel sealing plate, the first titanium alloy electrode and the insulating bracket form a first interlayer cavity as a whole. The second stainless steel sealing plate, the second titanium alloy electrode and the insulating bracket form a second interlayer cavity as a whole. The first interlayer cavity and the second interlayer cavity are connected through a connecting hole. The connecting hole is on the insulating bracket, connecting the first interlayer cavity and the second interlayer cavity. The bottom of the first interlayer cavity is connected to the refrigerant inlet, and the bottom of the second interlayer cavity is connected to the refrigerant outlet. When in use, the refrigerant is fed in and out through the connecting holes on the insulating bracket for temperature control.

[0013] Preferably, a first threaded screw is welded in the middle of the first titanium alloy electrode, the first threaded screw is threadedly connected to the first stainless steel sealing plate, and is fixed by a first inner locking nut; a second threaded screw is welded in the middle of the second titanium alloy electrode, the second threaded screw is threadedly connected to the second stainless steel sealing plate, and is fixed by a second inner locking nut.

[0014] Preferably, a first stainless steel sealing plate screw hole is provided on the first stainless steel sealing plate, and the first stainless steel sealing plate is fixed to the outer edge of the insulating bracket by a first sealing insulating screw; a second stainless steel sealing plate screw hole is provided on the second stainless steel sealing plate, and the second stainless steel sealing plate is fixed to the outer edge of the insulating bracket by a second sealing insulating screw.

[0015] Preferably, the berry material has an electrical conductivity of 1800-4000 μS / cm.

[0016] More preferably, the berry material is grapes or mulberries. The berry material is transported by a material pump with a pump flow parameter of 0.5-3.0 t / h. The material pump can be a screw pump or a self-priming pump.

[0017] Preferably, the refrigerant temperature in the cooling device is set to -5°C to 8°C, with a refrigerant liquid flow rate of 300-500 L / h. After the refrigerant circulates for 5-10 minutes, the temperature reaches below the set temperature of 0°C, and a thin layer of ice crystals covers the electrode surface. The refrigerant is an 8%-10% alcohol-water solution.

[0018] Preferably, the berry material feed temperature is 15°C-30°C, and the berry discharge temperature is achieved by adjusting the electrode refrigerant liquid flow rate and temperature and the material feed rate to achieve the material discharge temperature being consistent with the feed temperature, that is, there is no temperature increase during the material processing process.

[0019] Preferably, the pulse electric field parameters are: pulse electric field intensity 0.6-2.2 kV / cm, frequency 200-500 Hz, and pulse width 6-10µs.

[0020] Preferably, the processing chamber device is a round-to-square structure, that is, the pipeline connecting the material is a round structure, and the structure between the two titanium alloy electrode plates is a square structure, forming a parallel plate structure, and a groove for installing a sealing ring is provided between the titanium alloy electrode and the insulating bracket.

[0021] During production, berries (grapes, mulberries, etc., crushed and pressed into a pulp) are placed in a container. The material can be preheated or precooled in a pretreatment tank. The material's conductivity, temperature, and other parameters are measured, with an ideal conductivity of less than 4000 µS / cm. The material temperature is adjusted based on actual production needs. The tank is connected to the material pump and the processing chamber via pipes. The cooling water inlet and outlet are connected to the refrigeration unit. The processing chamber electrodes are connected to the output of a high-voltage pulse power supply. All equipment is safely and effectively grounded.

[0022] During processing and production, first turn on the cooling device and refrigeration unit, set the refrigerant temperature to -5°C-8°C, the refrigerant liquid flow rate to 300-500 L / h, and the pump flow parameter to 0.5-3.0 t / h. The pump can be a screw pump or a self-priming pump, and the pump flow parameter can be adjusted via a frequency converter. After the refrigerant circulates for 5-10 minutes, at which point a thin layer of small ice crystals covers the surface of the titanium alloy electrode in the processing chamber. Start the material conveying pump, set the high-voltage pulse parameters, and start the high-voltage pulse power output. Fiber optics are used to measure the feed and discharge temperatures of the materials entering and leaving the processing chamber. The material conveying pump delivery speed is adjusted according to the material inlet and outlet temperatures. As the temperature rises, the material flow rate increases accordingly. The pump flow parameter adjustment is coordinated with the material feed and discharge temperatures to ensure that the material feed and discharge temperatures are consistent.

[0023] Preferably, the active ingredient in the berry extract containing active ingredients is polyphenol.

[0024] Compared with the prior art, the present invention has the following advantages:

[0025] 1. This continuous high-voltage pulsed electric field treatment method for enhanced extraction of active ingredients from berries is suitable for industrial extraction. The parallel-plate treatment chamber used in this method creates a relatively uniform electric field and can apply large high-voltage pulses without generating local breakdown currents. The high-voltage pulses have high instantaneous power, reaching megawatts, but low average power (kilowatts), resulting in a short effective treatment time and virtually no temperature rise or thermal effects.

[0026] 2. The processing chamber used in this extraction method is composed of corrosion-resistant titanium alloy electrode plates and highly insulating material polytetrafluoroethylene. Polytetrafluoroethylene insulating material is embedded in the edge of the titanium alloy electrode to avoid the edge discharge breakdown effect under high voltage. At the same time, it is combined with synchronous cooling low-temperature electrode protection and bipolar symmetrical square wave to reduce corrosion and improve the life of the titanium alloy electrode.

[0027] 3. The high-voltage pulsed electric field treatment chamber used in this extraction method can be quickly connected according to specific process requirements. This system can handle a wide range of materials, particularly berry extraction, offering strong operability, a wide range of adjustable parameters, and easy cleaning and disassembly. The dimensions of the relevant material external connectors are all standard, allowing multiple treatment chambers to be connected in series according to actual production needs. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 is a schematic diagram of the structure of a high-voltage pulse electric field connection extraction device and a material control system;

[0029] FIG2 is a schematic diagram of the structure of the various components of the high-voltage pulse electric field treatment chamber device;

[0030] Explanation of the accompanying drawings: 1. High-voltage pulse power supply; 2. Material conveying pump; 3. Processing chamber device; 3-1. First stainless steel sealing plate screw hole; 3-2. First stainless steel sealing plate; 3-3. First outer locking nut; 3-4. First titanium alloy electrode; 3-5. First polytetrafluoroethylene bracket threaded hole; 3-6. Pipe connection threaded hole; 3-7. Connecting hole; 3-8. First inner electrode groove; 3-9. Contact processing area; 3-10. Second threaded screw; 3-11. Second inner locking nut; 3-12. Refrigerant inlet; 3-13. Refrigerant outlet; 4. Cooling device. Modes for Carrying Out the Invention

[0031] The following examples are provided to further illustrate the present invention, but are not intended to limit the present invention.

[0032] Unless otherwise defined, all technical terms used hereinafter have the same meanings as those generally understood by those skilled in the art. The technical terms used herein are for the purpose of describing specific embodiments only and are not intended to limit the scope of protection of the present invention. Unless otherwise specified, the experimental materials and reagents herein are conventional commercial products in the art.

[0033] As shown in Figures 1 and 2, a method for enhanced extraction of functional active ingredients from berries using continuous high-voltage pulsed electric field treatment is implemented by connecting an extraction device with a material control system using a high-voltage pulsed electric field. The method comprises a high-voltage pulsed electric field connecting the extraction device and the material control system, which includes a high-voltage pulsed electric field power supply 1, a material delivery pump 2, a processing chamber 3, and a cooling device 4. The high-voltage pulsed electric field power supply 1 provides power for the high-voltage pulsed electric field in the processing chamber 3. The berry material is delivered to the processing chamber 3 for processing via the material delivery pump 2. The processing chamber comprises a titanium alloy electrode, stainless steel sealing plates disposed on both sides, and an insulating bracket disposed in the middle. The titanium alloy electrode is symmetrically embedded in the insulating bracket, and the stainless steel sealing plate is fixed to the outer edge of the insulating bracket. The stainless steel sealing plate, titanium alloy electrode, and insulating bracket form a sandwich cavity. The bottom of the sandwich cavity is connected to a refrigerant inlet 3-12 and a refrigerant outlet 3-13 of a cooling device. Refrigerant enters the sandwich cavity through the refrigerant inlet 3-12 to cool the titanium alloy electrode, and is then discharged through the refrigerant outlet 3-13. The output end of the high-voltage pulsed electric field power supply 1 is connected to the titanium alloy electrode to generate a high-voltage pulsed electric field.

[0034] The titanium alloy electrodes include a first titanium alloy electrode 3-4 and a second titanium alloy electrode symmetrically embedded in an insulating bracket. The stainless steel sealing plates include a first stainless steel sealing plate 3-2 and a second stainless steel sealing plate symmetrically arranged on either side of the processing chamber device. The first stainless steel sealing plate 3-2, the first titanium alloy electrode 3-4, and the insulating bracket collectively form a first interlayer cavity, while the second stainless steel sealing plate, the second titanium alloy electrode, and the insulating bracket collectively form a second interlayer cavity. The first interlayer cavity and the second interlayer cavity are connected by a connecting hole 3-7. The bottom of the first interlayer cavity is connected to the refrigerant inlet 3-12, and the bottom of the second interlayer cavity is connected to the refrigerant outlet 3-13. A first threaded screw is welded to the center of the first titanium alloy electrode, which is threadedly connected to the first stainless steel sealing plate 3-2 and secured by a first inner locking nut. A second threaded screw 3-10 is welded to the center of the second titanium alloy electrode, which is threadedly connected to the second stainless steel sealing plate and secured by a second inner locking nut 3-11. A first stainless steel sealing plate screw hole 3-1 is provided on the first stainless steel sealing plate 3-2, and the first stainless steel sealing plate 3-2 is fixed to the outer edge of the insulating bracket by the first sealing insulating screw. A second stainless steel sealing plate screw hole is provided on the second stainless steel sealing plate, and the second stainless steel sealing plate is fixed to the outer edge of the insulating bracket by the second sealing insulating screw.

[0035] The high-voltage pulse power supply 1 can generate positive and negative pulse voltages of 0-25kV, with a maximum current resistance of 1000A. The rising edge of the high-voltage pulse waveform is less than 500ns, and the falling edge of the pulse waveform is less than 500ns. It can achieve a pulse width output of 1-20µs and a frequency output of 1-1000Hz.

[0036] In the following embodiment, the two output terminals of the high-voltage pulse power supply are directly connected via wires to the first outer locking nut 3-3 of the first titanium alloy electrode 3-4 and the second outer locking nut of the second titanium alloy electrode of the processing chamber device 3. The processing chamber device 3 has a round-to-square structure, and the entire bracket insulation material is polytetrafluoroethylene (external dimensions 200 mm × 200 mm × 200 mm). The insulating bracket has threaded pipe connection holes 3-6 at the top and bottom to facilitate connection to external pipes. The internal hole size is 100 mm in diameter.

[0037] Titanium alloy electrodes are embedded on the left and right sides of the insulating bracket (inner electrodes, with a symmetrical structure on the left and right sides). There is a first inner electrode groove 3-8 between the first titanium alloy electrode 3-4 and the insulating bracket. A sealing ring is installed in the middle of the first inner electrode groove 3-8. The size of the first titanium alloy electrode 3-4 is 80 mm × 80 mm × 5 mm (length × width × thickness). The contact area between the first titanium alloy electrode 3-4 and the polytetrafluoroethylene insulating bracket is 50 mm × 50 mm, that is, the effective contact processing area 3-9 of the left and right parallel plate titanium alloy electrodes is 50 mm × 50 mm. The inner electrode titanium alloy electrode has a left-right symmetrical structure, and the distance between the left and right titanium alloy electrodes (that is, the first titanium alloy electrode and the second titanium alloy electrode) is 100 mm. There is a second inner electrode groove between the second titanium alloy electrode and the insulating bracket, and a sealing ring is installed in the middle of the second inner electrode groove. The size of the second titanium alloy electrode 3-4 is 80 mm × 80 mm × 5 mm (length × width × thickness), the contact area between the second titanium alloy electrode and the polytetrafluoroethylene insulating bracket is 50 mm × 50 mm, and the distance between the left and right titanium alloy electrodes (i.e., the first titanium alloy electrode and the second titanium alloy electrode) is 100 mm.

[0038] A first threaded screw is welded in the middle of the first titanium alloy electrode 3-4 of the inner electrode, and the first threaded screw is connected to the first stainless steel sealing plate 3-2 by threads (the stainless steel sealing plate has dimensions of 200 mm×200 mm×5 mm, length×width×thickness) and is fixed by a first inner locking nut. A second threaded screw 3-10 is welded in the middle of the second titanium alloy electrode of the inner electrode, and the second threaded screw 3-10 is connected to the second stainless steel sealing plate by threads (the stainless steel sealing plate has dimensions of 200 mm×200 mm×5 mm, length×width×thickness) and is fixed by a second inner locking nut 3-11.

[0039] The first stainless steel sealing plate 3-2 has a first stainless steel sealing plate screw hole 3-1 and a first polytetrafluoroethylene bracket screw hole 3-5, and the first stainless steel sealing plate 3-2 is fixed to the outer edge of the overall bracket through a sealing insulating screw (PEEK screw). The contact thickness between the outer edge of the bracket and the first stainless steel sealing plate 3-2 is 20 mm. The insulating bracket has a groove and a first polytetrafluoroethylene bracket screw hole 3-5, and a sealing ring is installed between the grooves. The first stainless steel sealing plate 3-2 has a first stainless steel sealing plate screw hole 3-1, which is tightened and fixed to the insulating bracket thread by an insulating screw (PEEK screw). After the first stainless steel sealing plate 3-2 is tightened and fixed to the inner electrode first titanium alloy electrode thread, it forms a left interlayer cavity with the bracket as a whole. The second stainless steel sealing plate has screw holes for the second stainless steel sealing plate and threaded holes for the second PTFE bracket. The second stainless steel sealing plate is secured to the outer edge of the integral bracket using sealed insulating screws (PEEK screws). The contact thickness between the outer edge of the bracket and the second stainless steel sealing plate is 20 mm. The insulating bracket has grooves and threaded holes for the second PTFE bracket. A sealing ring is installed between the grooves. The second stainless steel sealing plate has screw holes for the second stainless steel sealing plate. The insulating screws (PEEK screws) are used to tighten the second stainless steel sealing plate and the insulating bracket. The second stainless steel sealing plate is screwed to the inner electrode and the second titanium alloy electrode, forming a right interlayer cavity with the bracket. The overall structure of the processing chamber device forms a left and right interlayer cavity. The left and right interlayer cavities are connected by connecting holes 3-7. The holes are directly connected. The bottom of the left interlayer cavity is connected to the refrigerant inlet 3-12 of the external cooling device, and the bottom of the right interlayer cavity is connected to the refrigerant outlet 3-13 of the external cooling device. External cooling device 4 is connected to achieve synchronous circulation cooling. The specific processing chamber device is shown in Figure 2.

[0040] Example 1

[0041] As shown in Figure 1, a method for enhanced extraction of functional active ingredients from berries using continuous high-voltage pulsed electric field treatment is described. This method requires the support of a high-voltage pulse power supply 1, a material delivery pump 2, a processing chamber 3, a cooling unit 4, and other related devices. The various system components are connected and secured in the sequence shown in Figure 1. 200 kg of commercially available rose-scented grapes are purchased, destemmed, and crushed using a screw press. A conductivity meter measures the initial conductivity of the grape pulp, which is 1800 µS / cm. The initial temperature of the grape pulp is 20°C. The high-voltage pulse power supply output parameters are set to a pulsed electric field intensity of 0.6 kV / cm, a frequency of 500 Hz, a pulse width of 10 µs, and a bipolar symmetrical square wave pulse. Once these parameters are set, the process is ready for startup. The grape pulp is then piped to the processing chamber 3 via material delivery pump 2, with feed from the bottom and discharge from the top. The cooling outlet of the treatment chamber was connected to a refrigeration unit, which used an 8% ethanol-water solution as the cooling fluid. The cooling fluid temperature was -5°C, the cooling fluid flow rate was 300 L / h, and the pump flow rate was 0.5 t / h. After connecting the treatment chamber to the refrigeration system, the cooling unit was first turned on and the refrigeration unit pipeline was circulated for approximately 5 minutes. Once a small ice crystal layer appeared on the surface of the titanium alloy inner electrode in the treatment chamber or the cooling fluid temperature in the cooling circulation system reached a stable set temperature, the material pump was started to deliver the grape slurry. After the grape slurry passed through the treatment chamber, the high-voltage pulse power supply was activated. Using a fiber optic thermometer, the grape slurry feed temperature and discharge temperature were both 20°C, indicating overall stability. No further pump flow rate adjustment was required. After treatment, the high-voltage pulse power supply was turned off, the material delivery pump was stopped, and the cooling system was shut down. The polyphenol content of the grape slurry obtained after treatment was measured, and the polyphenol content was 1208 mg / L. The polyphenol content of the untreated grape slurry was 1062 mg / L.

[0042] Comparative Example 1 (the application effect is not obvious at an electric field strength lower than 0.6 kV / cm)

[0043] As shown in Figure 1, a method for enhanced extraction of functional active ingredients from berries using a continuous high-voltage pulsed electric field treatment is described. This method requires the support of a high-voltage pulse power supply 1, a material delivery pump 2, a processing chamber 3, a cooling unit 4, and other related devices. The various system components are connected and secured in the sequence shown in Figure 1. 200 kg of commercially available rose-scented grapes are purchased, destemmed, and crushed using a screw press. A conductivity meter measures the initial conductivity of the grape pulp, which is 1800 µS / cm. The initial temperature of the grape pulp is 20°C. The high-voltage pulse power supply output parameters are set to a pulsed electric field intensity of 0.5 kV / cm, a frequency of 500 Hz, a pulse width of 10 µs, and a bipolar symmetrical square wave pulse. Once these parameters are set, the process is ready for startup. The grape pulp is then piped to the processing chamber 3 via material delivery pump 2, with feed from the bottom and discharge from the top. The cooling outlet of the treatment chamber was connected to a refrigeration unit, which used an 8% ethanol-water solution as the cooling fluid. The cooling fluid temperature was -5°C, the cooling fluid flow rate was 300 L / h, and the material pump flow rate was 0.5 t / h. After connecting the treatment chamber to the refrigeration system, the cooling unit was first turned on and the refrigeration unit pipeline was circulated for approximately 5 minutes. Once a small ice crystal layer appeared on the surface of the titanium alloy inner electrode in the treatment chamber or the cooling fluid temperature in the cooling circulation system reached a stable set temperature, the material pump was started to deliver grape slurry. After the grape slurry passed through the treatment chamber, the high-voltage pulse power supply was activated. Using a fiber optic thermometer, the grape slurry feed temperature and discharge temperature were both 20°C, indicating overall stability. No further pump flow rate adjustment was required. After treatment, the high-voltage pulse power supply was turned off, the material pump was stopped, and the cooling system was shut down. The polyphenol content of the grape slurry obtained after treatment was measured, and the polyphenol content was 1073 mg / L. The polyphenol content of the untreated grape slurry was 1058 mg / L.

[0044] Comparative Example 2 (the application effect is not obvious when the frequency is lower than 200Hz)

[0045] As shown in Figure 1, a method for enhanced extraction of functional active ingredients from berries using continuous high-voltage pulsed electric field treatment is described. This method requires the support of a high-voltage pulse power supply 1, a material delivery pump 2, a processing chamber 3, a cooling unit 4, and other related devices. The various system components are connected and secured in the sequence shown in Figure 1. 200 kg of commercially available rose-scented grapes are purchased, destemmed, and crushed using a screw press. A conductivity meter measures the initial conductivity of the grape pulp, which is 1804 µS / cm. The initial temperature of the grape pulp is 20°C. The high-voltage pulse power supply output parameters are set to a pulsed electric field intensity of 0.6 kV / cm, a frequency of 190 Hz, a pulse width of 10 µs, and a bipolar symmetrical square wave pulse. Once these parameters are set, the process is ready for startup. The grape pulp is piped to the processing chamber 3 via material delivery pump 2, with feed from the bottom and discharge from the top. The cooling outlet of the treatment chamber was connected to a refrigeration unit, which used an 8% ethanol-water solution as the cooling circulating fluid. The cooling fluid temperature was maintained at -5°C, the cooling fluid flow rate was 300 L / h, and the material pump flow rate was set at 0.5 t / h. After connecting the treatment chamber to the refrigeration system, the cooling unit was first turned on and the refrigeration unit pipeline was circulated for approximately 5 minutes. Once a small ice crystal layer appeared on the surface of the titanium alloy inner electrode in the treatment chamber or the cooling fluid temperature in the cooling circulation system reached a stable setpoint, the material pump was started to deliver grape slurry. After the grape slurry passed through the treatment chamber, the high-voltage pulse power supply was activated. Using a fiber optic thermometer, the grape slurry feed temperature and discharge temperature were both 20°C, indicating overall stability, and no pump flow rate adjustment was required. After treatment, the high-voltage pulse power supply was turned off, the material pump was stopped, and the cooling system was shut down. The polyphenol content of the grape slurry obtained after treatment was measured, and the polyphenol content was 1086 mg / L. The polyphenol content of the untreated grape slurry was 1049 mg / L.

[0046] Example 2

[0047] As shown in Figure 1, a method for enhanced extraction of functional active ingredients from berries using continuous high-voltage pulsed electric field treatment is described. This method requires the support of a high-voltage pulse power supply 1, a material delivery pump 2, a processing chamber 3, a cooling unit 4, and other related devices. The various system components are connected and secured in the sequence shown in Figure 1. 200 kg of commercially available rose-scented grapes are purchased, destemmed, and crushed using a screw press. A conductivity meter measures the initial conductivity of the grape pulp, which is 1803 µS / cm. The initial temperature of the grape pulp is 20°C. The high-voltage pulse power supply output parameters are set to a pulsed electric field intensity of 0.7 kV / cm, a frequency of 500 Hz, a pulse width of 8 µs, and a bipolar symmetrical square wave pulse. Once these parameters are set, the process is ready for startup. The grape pulp is piped to the processing chamber 3 via material delivery pump 2, with feed from the bottom and discharge from the top. The cooling port (number 3) in the treatment chamber was connected to a refrigeration unit. The cooling fluid was an 8% ethanol-water solution at a temperature of -8°C and a flow rate of 350 L / h. The material pump flow rate was set at 0.6 t / h. After connecting the treatment chamber to the refrigeration system, the cooling unit was first turned on, and the refrigeration unit pipeline was circulated for approximately 5 minutes. Once a small ice crystal layer appeared on the surface of the titanium alloy inner electrode in the treatment chamber or the coolant temperature in the cooling circulation system reached a stable setpoint, the material pump was started to deliver grape slurry. After the grape slurry passed through the treatment chamber, the high-voltage pulse power supply was activated. A fiber optic thermometer was used to measure the grape slurry's inlet and outlet temperatures, both at 20°C and 20°C, indicating overall stability. No further pump flow rate adjustment was required. After treatment, the high-voltage pulse power supply was turned off, the material pump was stopped, and the cooling system was shut down. The polyphenol content of the treated grape slurry was measured, revealing a polyphenol content of 1253 mg / L. The polyphenol content in the untreated grape slurry was 1056 mg / L.

[0048] Example 3

[0049] As shown in Figure 1, a method for enhanced extraction of functional active ingredients from berries using continuous high-voltage pulsed electric field treatment is described. This method requires the support of a high-voltage pulse power supply 1, a material delivery pump 2, a processing chamber 3, a cooling unit 4, and other related devices. The various system components are connected and secured in the sequence shown in Figure 1. 200 kg of commercially available rose-scented grapes are purchased, destemmed, and crushed using a screw press. A conductivity meter measures the initial conductivity of the grape pulp, which is 1812 µS / cm. The initial temperature of the grape pulp is 25°C. The high-voltage pulse power supply output parameters are set to a pulsed electric field intensity of 0.8 kV / cm, a frequency of 500 Hz, a pulse width of 9 µs, and a bipolar symmetrical square wave pulse. Once these parameters are set, the process is ready for startup. The grape pulp is then piped to the processing chamber 3 via material delivery pump 2, with feed from the bottom and discharge from the top. The cooling outlet of the treatment chamber was connected to a refrigeration unit, which used an 8% ethanol-water solution as the cooling circulating fluid. The cooling fluid temperature was -7°C, the cooling fluid flow rate was 410 L / h, and the material pump flow rate was 1.0 t / h. After connecting the treatment chamber to the refrigeration system, the cooling unit was first turned on and the refrigeration unit pipeline was circulated for approximately 5 minutes. Once a small ice crystal layer appeared on the surface of the titanium alloy inner electrode in the treatment chamber or the cooling fluid temperature in the cooling circulation system reached a stable set temperature, the material pump was started to deliver grape slurry. After the grape slurry passed through the treatment chamber, the high-voltage pulse power supply was activated. Using a fiber optic thermometer, the grape slurry feed temperature and discharge temperature were both 25°C, indicating overall stability. No further pump flow rate adjustment was required. After treatment, the high-voltage pulse power supply was turned off, the material pump was stopped, and the cooling system was shut down. The polyphenol content of the grape slurry obtained after treatment was measured, revealing a polyphenol content of 1291 mg / L. The polyphenol content of the untreated grape slurry was 1087 mg / L.

[0050] Example 4

[0051] As shown in Figure 1, a method for enhanced extraction of functional active ingredients from berries using continuous high-voltage pulsed electric field treatment is described. This method requires the support of a high-voltage pulse power supply 1, a material delivery pump 2, a processing chamber 3, a cooling unit 4, and other related devices. The various system components are connected and secured in the sequence shown in Figure 1. 200 kg of commercially available rose-scented grapes are purchased, destemmed, and crushed using a screw press. A conductivity meter measures the initial conductivity of the grape pulp, which is 1807 µS / cm. The initial temperature of the grape pulp is 28°C. The high-voltage pulse power supply output parameters are set to a pulsed electric field strength of 1.2 kV / cm, a frequency of 400 Hz, a pulse width of 8 µs, and a bipolar symmetrical square wave pulse. After these parameters are set, the process is ready for startup. The grape pulp is piped to the processing chamber 3 via material delivery pump 2, with feed from the bottom and discharge from the top. The cooling outlet of the treatment chamber was connected to a refrigeration unit, which used an 8% ethanol-water solution as the cooling fluid. The cooling fluid temperature was -6°C, the cooling fluid flow rate was 385 L / h, and the material pump flow rate was 1.2 t / h. After connecting the treatment chamber to the refrigeration system, the cooling unit was first turned on and the refrigeration unit pipeline was circulated for approximately 5 minutes. Once a small ice crystal layer appeared on the surface of the titanium alloy inner electrode in the treatment chamber or the cooling fluid temperature in the cooling circulation system reached a stable set temperature, the material pump was started to deliver grape slurry. After the grape slurry passed through the treatment chamber, the high-voltage pulse power supply was activated. Using a fiber optic thermometer, the grape slurry feed temperature and discharge temperature were both 28°C, indicating overall stability. No further pump flow rate adjustment was required. After treatment, the high-voltage pulse power supply was turned off, the material pump was stopped, and the cooling system was shut down. The polyphenol content of the grape slurry obtained after treatment was measured, and the polyphenol content was 1274 mg / L. The polyphenol content of the untreated grape slurry was 1030 mg / L.

[0052] Example 5

[0053] As shown in Figure 1, a method for enhanced extraction of functional active ingredients from berries using continuous high-voltage pulsed electric field treatment is described. This method requires the support of a high-voltage pulse power supply 1, a material delivery pump 2, a processing chamber 3, a cooling unit 4, and other related devices. The various system components are connected and secured in the sequence shown in Figure 1. 200 kg of commercially available rose-scented grapes are purchased, destemmed, and crushed using a screw press. A conductivity meter measures the initial conductivity of the grape pulp, which is 1822 µS / cm. The initial temperature of the grape pulp is 30°C. The high-voltage pulse power supply output parameters are set to a pulsed electric field strength of 1.5 kV / cm, a frequency of 400 Hz, a pulse width of 7 µs, and a bipolar symmetrical square wave pulse. Once these parameters are set, the process is ready for startup. The grape pulp is piped to the processing chamber 3 via material delivery pump 2, with feed from the bottom and discharge from the top. The cooling outlet of the treatment chamber was connected to a refrigeration unit, which used an 8% ethanol-water solution as the cooling fluid. The cooling fluid temperature was -8°C, the cooling fluid flow rate was 500 L / h, and the material pump flow rate was 1.5 t / h. After connecting the treatment chamber to the refrigeration system, the cooling unit was first turned on and the refrigeration unit pipeline was circulated for approximately 5 minutes. Once a small ice crystal layer appeared on the surface of the titanium alloy inner electrode in the treatment chamber or the cooling fluid temperature in the cooling circulation system reached a stable set temperature, the material pump was started to deliver grape slurry. After the grape slurry passed through the treatment chamber, the high-voltage pulse power supply was activated. Using a fiber optic thermometer, the grape slurry feed temperature and discharge temperature were both 30°C, indicating overall stability. No further pump flow rate adjustment was required. After treatment, the high-voltage pulse power supply was turned off, the material pump was stopped, and the cooling system was shut down. The polyphenol content of the grape slurry obtained after treatment was measured, and the polyphenol content was 1482 mg / L. The polyphenol content of the untreated grape slurry was 1074 mg / L.

[0054] Example 6

[0055] As shown in Figure 1, a method for enhanced extraction of functional active ingredients from berries using a continuous high-voltage pulsed electric field treatment is described. This method requires the support of a high-voltage pulse power supply 1, a material delivery pump 2, a processing chamber 3, a cooling unit 4, and other related devices. The various system components are connected and secured in the sequence shown in Figure 1. 200 kg of commercially available rose-scented grapes are purchased, stemmed, and leafed, then crushed using a screw press. A conductivity meter measures the initial conductivity of the grape pulp at 1800 µS / cm, which is then adjusted to 1957 µS / cm by adding a small amount of saline. The initial temperature of the grape pulp is 15°C. The high-voltage pulse power supply output parameters are set to a pulsed electric field strength of 2.0 kV / cm, a frequency of 300 Hz, a pulse width of 6 µs, and a bipolar symmetrical square wave pulse. Once these parameters are set, the process is ready for startup. The grape pulp is piped to the processing chamber 3 via material delivery pump 2, with feed from the bottom and discharge from the top. The cooling outlet of the treatment chamber was connected to a refrigeration unit, which used an 8% ethanol-water solution as the cooling circulating fluid. The cooling fluid temperature was -8°C, the cooling fluid flow rate was 480 L / h, and the material pump flow rate was 1.8 t / h. After connecting the treatment chamber to the refrigeration system, the cooling unit was first turned on and the refrigeration unit pipeline was circulated for approximately 5 minutes. Once a small ice crystal layer appeared on the surface of the titanium alloy inner electrode in the treatment chamber or the cooling fluid temperature in the cooling circulation system reached a stable set temperature, the material pump was started to deliver grape slurry. After the grape slurry passed through the treatment chamber, the high-voltage pulse power supply was activated. Using a fiber optic thermometer, the grape slurry feed temperature and discharge temperature were both 15°C, indicating overall stability, and no pump flow rate adjustment was required. After treatment, the high-voltage pulse power supply was turned off, the material pump was stopped, and the cooling system was shut down. The polyphenol content of the grape slurry obtained after treatment was measured, and the polyphenol content was 1376 mg / L. The polyphenol content of the untreated grape slurry was 1065 mg / L.

[0056] Example 7

[0057] As shown in Figure 1, a method for enhanced extraction of functional active ingredients from berries using continuous high-voltage pulsed electric field treatment is described. This method requires the support of a high-voltage pulse power supply 1, a material delivery pump 2, a processing chamber 3, a cooling unit 4, and other related devices. The various system components are connected and secured in the sequence shown in Figure 1. 200 kg of commercially available rose-scented grapes are purchased, stemmed, and leafed, then crushed using a screw press. A conductivity meter measures the initial conductivity of the grape pulp, which is 1831 µS / cm. A small amount of sodium chloride is added to adjust the conductivity to 2230 µS / cm. The initial temperature of the grape pulp is 15°C. The high-voltage pulse power supply output parameters are set to a pulsed electric field strength of 2.2 kV / cm, a frequency of 200 Hz, a pulse width of 9 µs, and a bipolar symmetrical square wave pulse. Once these parameters are set, the process is ready for startup. The grape pulp is piped to the processing chamber 3 via material delivery pump 2, with feed from the bottom and discharge from the top. The cooling outlet of the treatment chamber was connected to a refrigeration unit, which used an 8% ethanol-water solution as the cooling fluid. The cooling fluid temperature was -8°C, the cooling fluid flow rate was 450 L / h, and the material pump flow rate was 2.5 t / h. After connecting the treatment chamber to the refrigeration system, the cooling unit was first turned on and the refrigeration unit pipeline was circulated for approximately 5 minutes. Once a small ice crystal layer appeared on the surface of the titanium alloy inner electrode in the treatment chamber or the cooling fluid temperature in the cooling circulation system reached a stable set temperature, the material pump was started to deliver grape slurry. After the grape slurry passed through the treatment chamber, the high-voltage pulse power supply was activated. Using a fiber optic thermometer, the grape slurry feed temperature and discharge temperature were both 15°C, indicating overall stability. No further pump flow rate adjustment was required. After treatment, the high-voltage pulse power supply was turned off, the material pump was stopped, and the cooling system was shut down. The polyphenol content of the grape slurry obtained after treatment was measured, and the polyphenol content was 1345 mg / L. The polyphenol content of the untreated grape slurry was 1071 mg / L.

[0058] Example 8

[0059] As shown in Figure 1, a method for enhanced extraction of functional active ingredients from berries using continuous high-voltage pulsed electric field treatment is described. This method requires the support of a high-voltage pulse power supply 1, a material delivery pump 2, a processing chamber 3, a cooling unit 4, and other related devices. The various system components are connected and secured in the sequence shown in Figure 1. 200 kg of commercially available rose-scented grapes are purchased, destemmed, and crushed using a screw press. A conductivity meter measures the initial conductivity of the grape pulp, which is 1820 µS / cm. A small amount of sodium chloride is added to adjust the conductivity to 3120 µS / cm. The initial temperature of the grape pulp is 18°C. The high-voltage pulse power supply output parameters are set to a pulsed electric field strength of 2.5 kV / cm, a frequency of 300 Hz, a pulse width of 6 µs, and a bipolar symmetrical square wave pulse. Once these parameters are set, the process is ready for startup. The grape pulp is piped to the processing chamber 3 via material delivery pump 2, with feed from the bottom and discharge from the top. The cooling outlet of the treatment chamber was connected to a refrigeration unit, which used an 8% ethanol-water solution as the cooling circulating fluid. The cooling fluid temperature was -8°C, the cooling fluid flow rate was 435 L / h, and the material pump flow rate was 3.0 t / h. After connecting the treatment chamber to the refrigeration system, the cooling unit was first turned on and the refrigeration unit pipeline was circulated for approximately 5 minutes. Once a small ice crystal layer appeared on the surface of the titanium alloy inner electrode in the treatment chamber or the cooling fluid temperature in the cooling circulation system reached a stable set temperature, the material pump was started to deliver grape slurry. After the grape slurry passed through the treatment chamber, the high-voltage pulse power supply was activated. Using a fiber optic thermometer, the grape slurry feed temperature and discharge temperature were both 18°C, indicating overall stability. No further pump flow rate adjustment was required. After treatment, the high-voltage pulse power supply was turned off, the material pump was stopped, and the cooling system was shut down. The polyphenol content of the grape slurry obtained after treatment was measured, revealing a polyphenol content of 1307 mg / L. The polyphenol content of the untreated grape slurry was 1060 mg / L. The treatment intensity was greater than 2.5 kV / cm, and the polyphenol yield was lower than that at 2.2 kV.

[0060] Example 9

[0061] As shown in Figure 1, a method for enhanced extraction of functional active ingredients from berries using a continuous high-voltage pulsed electric field treatment is described. This method requires the support of a high-voltage pulse power supply 1, a material delivery pump 2, a processing chamber 3, a cooling unit 4, and other related devices. The various system components were connected and secured in the order shown in Figure 1. 200 kg of mulberries were crushed using a screw press. The initial conductivity of the mulberry pulp was measured to be 3670 µS / cm, and the material temperature was set at 18°C. The high-voltage pulse power supply output parameters were set to a pulsed electric field intensity of 1.1 kV / cm, a frequency of 200 Hz, a pulse width of 8 µs, and a bipolar symmetrical square wave pulse. After these parameters were set, the process was started. The mulberry pulp was delivered to the processing chamber 3 via a pipeline via the material delivery pump 2, with feed from the bottom and discharge from the top. The cooling unit port of the processing chamber was connected to a refrigeration unit, which used a 10% ethanol-water solution as the cooling fluid. The cooling fluid temperature was -8°C, the cooling fluid flow rate was 450 L / h, and the material pump flow rate was 1.0 t / h. After connecting the treatment chamber to the refrigeration system, the cooling device was first turned on and the refrigeration unit pipeline was circulated for approximately 5 minutes. Waiting for a small ice crystal layer to appear on the surface of the titanium alloy inner electrode in the treatment chamber or for the coolant temperature in the cooling circulation system to reach a stable set temperature, the material pump was started to deliver the mulberry slurry. After the mulberry slurry passed through the treatment chamber, the high-voltage pulse power supply output was activated. A fiber optic thermometer was used to measure the grape slurry feed temperature and discharge temperature to be 18°C, which met the overall stability requirements and did not require any adjustment of the pump flow rate. After the treatment was completed, the high-voltage pulse power supply output was turned off, the material delivery pump was stopped, and the cooling system was closed. The polyphenol content was measured after treatment and was 2363 mg / L. The polyphenol content in the sample that had not been treated with the pulsed electric field was 1857 mg / L.

[0062] Example 10

[0063] As shown in Figure 1, a method for enhanced extraction of functional active ingredients from berries using continuous high-voltage pulsed electric field treatment is described. This method requires the support of a high-voltage pulse power supply 1, a material delivery pump 2, a processing chamber 3, and a cooling unit 4, among other related devices. The various system components were connected and secured in the order shown in Figure 1. 200 kg of mulberries were crushed using a screw press. The initial conductivity of the mulberry pulp was measured to be 3320 µS / cm. The initial material temperature was 25°C. The high-voltage pulse power supply output parameters were set to a pulsed electric field intensity of 2.0 kV / cm, a frequency of 200 Hz, a pulse width of 6 µs, and a bipolar symmetrical square wave pulse. After these parameters were set, the process was started. The mulberry pulp was delivered to the processing chamber 3 via a pipeline via the material delivery pump 2, with feed from the bottom and discharge from the top. The cooling unit port of the processing chamber was connected to a refrigeration unit, which used a 10% ethanol-water solution as the cooling fluid. The cooling fluid temperature was -5°C, the cooling fluid flow rate was 500 L / h, and the material pump flow rate was 3.0 t / h. After connecting the treatment chamber to the refrigeration system, the cooling device was first turned on and the refrigeration unit pipeline was circulated for approximately 5 minutes. Waiting for a small ice crystal layer to appear on the surface of the titanium alloy inner electrode in the treatment chamber or for the coolant temperature in the cooling circulation system to reach a stable set temperature, the material pump was started to deliver the mulberry slurry. After the mulberry slurry passed through the treatment chamber, the high-voltage pulse power supply was activated. A fiber optic thermometer was used to measure the grape slurry feed temperature and discharge temperature to be 25°C, which met the overall stability requirements and no pump flow rate adjustment was required. After the treatment was completed, the high-voltage pulse power supply was turned off, the material delivery pump was stopped, and the cooling system was closed. The polyphenol content was measured after treatment and was 2250 mg / L. The polyphenol content in the sample that had not been treated with the pulsed electric field was 1894 mg / L.

[0064] In terms of synchronous low-temperature treatment of electrodes, the experiment compared 50 L of sodium chloride solution with a conductivity of 4000 µS / cm. The experiment was run continuously for 4 hours without and with electrode cooling. The titanium ion concentration in the solution was measured (using inductively coupled plasma mass spectrometry). The experimental results are shown in Table 1 below:

[0065] Table 1

[0066] Electric field strength Pulse width frequency Titanium ion concentration Electrode cooling Solution feed temperature 2.0 kV / cm 10 µs 500 Hz Not detected (below the detection limit of 0.4 µg / L) Electrode cooling, maintaining the electrode temperature at or below 0°C, the electrode surface is covered with an ice film 20°C 2.2 kV / cm 10 µs 500 Hz Not detected (below the detection limit of 0.4 µg / L) Electrode cooling, maintaining the electrode temperature at around 0°C, the surface is covered with an ice film 20°C 2.2 kV / cm 10 µs 450 Hz Not detected (below the detection limit of 0.4 µg / L) Electrode cooling, maintaining the electrode temperature at or below 0°C, the surface is covered with an ice film 20°C 2.2 kV / cm 10 µs 600 Hz 6.41 µg / L Electrode cooling, maintaining the electrode temperature at or below 0°C, the electrode surface is covered with an ice film 20°C 2.2 kV / cm 10 µs 500 Hz 28.73 µg / L Without cooling 20℃ 2.2kV / cm 12µs 500Hz 22.57µg / L Electrode cooling, keep the electrode temperature at 0℃ or below, the electrode surface is covered with ice film 20℃ 2.4kV / cm 10µs 500Hz 33.71µg / L Electrode cooling, keep the electrode temperature at 0℃ or below, the electrode surface is covered with ice film 20℃ 2.5kV / cm 10µs 500Hz 43.52µg / L Electrode cooling, keep the electrode temperature at 0℃ or below, the electrode surface is covered with ice film 20℃

[0067] Under conditions of an electric field strength of 2.2 kV / cm, a bipolar pulse width of 10µs, and a frequency of ≤500Hz, cooling the electrode can significantly improve its corrosion resistance. Electrode strengths greater than 2.2 kV / cm, or pulse widths ≥12µs, can cause corrosion.

[0068] The description of the above embodiments is only used to help understand the technical solution and core ideas of the present invention. It should be pointed out that for those skilled in the art, several improvements and modifications can be made to the present invention without departing from the principles of the present invention. These improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A method for intensified extraction of functional active ingredients from berries by continuous high-voltage pulse electric field treatment, characterized in that: The extraction device is connected to the material control system by a high-voltage pulse electric field. The extraction device is connected to the material control system by a high-voltage pulse electric field. The extraction device is connected to the material control system by a high-voltage pulse electric field. The extraction device includes a processing chamber device and a cooling device. The processing chamber device includes a titanium alloy electrode, a stainless steel sealing plate arranged on both sides, and an insulating bracket arranged in the middle. The titanium alloy electrode is symmetrically embedded in the insulating bracket. The stainless steel sealing plate is fixed to the outer edge of the insulating bracket. The stainless steel sealing plate, the titanium alloy electrode and the insulating bracket form a sandwich cavity as a whole. The bottom of the sandwich cavity is connected to a refrigerant inlet and a refrigerant outlet of an external cooling device. The refrigerant enters the sandwich cavity through the refrigerant inlet to cool the titanium alloy electrode, and then the refrigerant is discharged through the refrigerant outlet. The output end of the high-voltage pulse power supply is connected to the titanium alloy electrode to generate a high-voltage pulse electric field. The method comprises the following steps: after the processing chamber device is cooled by the cooling device to reach a set temperature, the output parameters of the high-voltage pulse power supply are set, the high-voltage pulse power supply generates a bipolar symmetrical square wave, the pulse electric field parameters are set, and the distance between the two titanium alloy electrodes is 100 mm, transporting the pretreated berry material to a processing chamber device, subjecting the berry material to a high-voltage pulse electric field treatment, wherein the feeding temperature and the discharging temperature of the berry material are the same, and obtaining a berry extract containing active ingredients.

2. The method according to claim 1, characterized in that The titanium alloy electrode comprises a first titanium alloy electrode and a second titanium alloy electrode symmetrically embedded on an insulating bracket. The titanium alloy electrode material is Ti-0.2Pd corrosion-resistant and high-hardness titanium alloy. The stainless steel sealing plate comprises a first stainless steel sealing plate and a second stainless steel sealing plate symmetrically arranged on both sides of the processing chamber device. The first stainless steel sealing plate, the first titanium alloy electrode and the insulating bracket form a first interlayer cavity as a whole. The second stainless steel sealing plate, the second titanium alloy electrode and the insulating bracket form a second interlayer cavity as a whole. The first interlayer cavity and the second interlayer cavity are connected through a connecting hole. The connecting hole is on the insulating bracket, connecting the first interlayer cavity and the second interlayer cavity. The bottom of the first interlayer cavity is connected to the refrigerant inlet, and the bottom of the second interlayer cavity is connected to the refrigerant outlet. When in use, the refrigerant is fed in and out through the connecting hole on the insulating bracket for temperature control.

3. The method according to claim 2, characterized in that A first threaded screw is welded in the middle of the first titanium alloy electrode, the first threaded screw is threadedly connected to the first stainless steel sealing plate and fixed by a first inner locking nut; a second threaded screw is welded in the middle of the second titanium alloy electrode, the second threaded screw is threadedly connected to the second stainless steel sealing plate and fixed by a second inner locking nut.

4. The method according to claim 2, characterized in that: The first stainless steel sealing plate is provided with a first stainless steel sealing plate screw hole, and the first stainless steel sealing plate is fixed to the outer edge of the insulating bracket by the first sealing insulating screw. The second stainless steel sealing plate is provided with a second stainless steel sealing plate screw hole, and the second stainless steel sealing plate is fixed to the outer edge of the insulating bracket by the second sealing insulating screw.

5. The method according to claim 1 or 2, characterized in that: The berry material has an electrical conductivity of 1800-4000 µS / cm.

6. The method according to claim 5, characterized in that The berry material is grape or mulberry.

7. The method according to claim 1 or 2, characterized in that: The refrigerant temperature in the cooling device is set to -5℃-8℃, and the refrigerant liquid flow rate is 300-500 L / h. After the refrigerant circulates for 5-10 minutes, it reaches the set temperature below 0℃, and the electrode surface is covered with a thin layer of ice crystals.

8. The method according to claim 1 or 2, characterized in that: The berry material feed temperature is 15°C-30°C, and the berry discharge temperature is achieved by adjusting the electrode coolant liquid flow rate and temperature and the material feed rate to achieve the material discharge temperature being consistent with the feed temperature, that is, there is no temperature increase during the material processing process.

9. The method according to claim 1 or 2, characterized in that: The pulse electric field parameters are: pulse electric field intensity 0.6-2.2 kV / cm, frequency 200-500 Hz, pulse width 6-10 µs.

10. The method according to claim 1 or 2, characterized in that: The processing chamber device is a round-to-square structure, and a groove for installing a sealing ring is arranged between the titanium alloy electrode and the insulating bracket.

Images