Carrier for analysis, test strip for immunochromatography, and method for producing carrier for analysis

Abstract

This carrier (1) for analysis comprises: a porous body (10); and a support body (20) that supports the porous body (10) on one surface. The porous body (10) comprises a skeleton (11) that is formed by assembling a plurality of hollow particles (13), and a plurality of voids (12) that are surrounded by the skeleton (11). The hollow particles (13) each have an outer shell (14) that includes an anodized coating film containing aluminum oxide, and a cavity (15) that is surrounded by the outer shell (14). If the surface of the porous body (10) of the carrier (1) for analysis is measured by a measuring instrument in a state where the carrier (1) for analysis, in which the porous body (10) has absorbed water, is placed on a white reflection standard that is used for calibration of the measuring instrument, the L* value in the L*a*b* color system is 80 or more.

Description

Analytical carrier, immunochromatographic test strip, and method for manufacturing analytical carrier

[0001] The present disclosure relates to an analytical carrier, an immunochromatographic test strip, and a method for manufacturing the analytical carrier.

[0002] Immunochromatography is a known analytical method for analyzing an analyte carried on an analytical carrier. Lateral flow test kits using immunochromatography are also known. The test kits include a test strip containing a labeled antibody that reacts with the analyte and is labeled with labeling particles such as gold colloid particles and latex particles, and a capture antibody that reacts with the analyte and is immobilized at a predetermined site on the analytical carrier.

[0003] In this test kit, for example, a specimen containing an antigen to be analyzed is collected from a living organism, and a solution containing the specimen is dropped onto a predetermined location on the test kit. The antigen reacts with the labeled antibody to form a complex. The solution containing the complex then flows through the analytical carrier due to capillary action. Furthermore, as the solution containing the complex is developed by the analytical carrier, the antigen contained in the complex is captured by the capture antibody on the analytical carrier, and the complex accumulates at the capture site, causing color development. In this way, the degree of color development at the capture site with the complex supported by the analytical carrier can be visually confirmed to determine whether the specimen contains the antigen.

[0004] Patent Document 1 discloses an aluminum member including a porous body including a skeleton formed by an aggregation of a plurality of aluminum particles and a plurality of voids surrounded by the skeleton, the skeleton including an outer shell containing aluminum oxide, and the surface of the skeleton being formed by the outer shell. The aluminum member of Patent Document 1 includes an aluminum substrate that supports the porous body, and the porous body is provided on one or both sides of the substrate.

[0005] International Publication No. 2021 / 079813

[0006] The aluminum member of Patent Document 1 has high whiteness and water absorption performance, making it suitable for use as an immunochromatography test strip. However, in the aluminum member of Patent Document 1, the porous body becomes transparent when it absorbs a solution containing a sample, and the gray color of the aluminum substrate supporting the porous body can be seen through the porous body, causing the aluminum member to appear gray. As a result, it may not be easy to visually confirm the signal (line) on the aluminum member. Furthermore, even when reading the signal using a measuring device, the increased background may result in a reduced signal-to-noise ratio. In other words, in the aluminum member of Patent Document 1, when the porous body absorbs water, the distinguishability of the signal on the porous body may be reduced.

[0007] The present disclosure has been made in view of the problems inherent in the prior art, and an object of the present disclosure is to provide an analytical carrier, an immunochromatographic test strip, and a method for manufacturing the analytical carrier, which are capable of improving the discrimination ability during water absorption.

[0008] The analytical carrier according to the first aspect of the present disclosure comprises a porous body and a support supporting the porous body on one side. The porous body includes a skeleton formed by an assembly of a plurality of hollow particles and a plurality of voids surrounded by the skeleton. The hollow particles have an outer shell including an anodized film containing aluminum oxide, and a cavity surrounded by the outer shell. The skeleton is formed by the continuous outer shells of a plurality of hollow particles. The porosity of the porous body is 50% by volume or more and less than 100% by volume. The average pore diameter of the porous body is 0.1 μm or more and 20 μm or less. The analytical carrier having absorbed water is placed on a white reflection standard with which the measuring instrument is calibrated, and the L when the surface of the porous body on the analytical carrier is measured with the measuring instrument is ＊ a ＊ b ＊ L in color system ＊ The value is 80 or greater.

[0009] An immunochromatographic test strip according to a second aspect of the present disclosure includes an analytical carrier.

[0010] A third aspect of the present disclosure provides a method for producing an analytical carrier, comprising a porous body and a support supporting the porous body on one side. The porous body includes a skeleton formed by an assembly of multiple hollow particles and multiple voids surrounded by the skeleton. The hollow particles have an outer shell including an anodized film containing aluminum oxide and a cavity surrounded by the outer shell. The skeleton is formed by the continuous outer shells of the multiple hollow particles. The production method includes a sintering step of sintering multiple aluminum metal particles on an aluminum substrate to obtain a sintered material including the aluminum substrate and a sintered body in which the aluminum metal particles are stacked on the aluminum substrate. The production method also includes an anodizing step of anodizing the sintered material to form outer shells including an anodized film on the surfaces of the aluminum metal particles. The production method also includes a dissolving step of dissolving the aluminum metal particles surrounded by the outer shells. In the above production method, the anodizing step and the dissolving step are repeated to form a metal member in which a porous body is stacked on an aluminum substrate. The manufacturing method includes a lamination step of adhering a porous body of a metal member to a support to form a laminate in which an aluminum substrate, a porous body, and a support are laminated in this order. The manufacturing method also includes a peeling step of dissolving the aluminum substrate to peel it off from the laminate. The aluminum metal particles contain at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and aluminum alloys. The aluminum substrate contains at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and aluminum alloys. The average particle diameter of the plurality of aluminum metal particles is 0.1 μm or more and 20 μm or less. The packing ratio of the sintered body is 10% by volume or more and 60% by volume or less. The support before adhering the porous body is placed on a white reflection standard with a calibrated measuring instrument, and the L when the surface of the support to which the porous body is adhered is measured with a measuring instrument is ＊ a ＊ b ＊ L in color system ＊ The value is 80 or greater.

[0011] According to the present disclosure, it is possible to provide an analytical carrier, an immunochromatographic test strip, and a method for manufacturing an analytical carrier that can improve discrimination during water absorption.

[0012] FIG. 1 is a schematic cross-sectional view showing an example of an analytical carrier according to one embodiment. FIG. 2 is a perspective view showing an example of a test kit according to one embodiment. FIG. 3 is a cross-sectional view showing an example of a sintered material obtained by sintering a plurality of aluminum metal particles on an aluminum base. FIG. 4 is a cross-sectional view showing an example of a state in which the sintered material is anodized to form an outer shell including an anodized film on the surface of the aluminum metal particle. FIG. 5 is a cross-sectional view showing an example of a state in which the aluminum metal particle surrounded by the outer shell is dissolved. FIG. 6 is a cross-sectional view showing an example of a state in which a porous body of a metal member is adhered to a support to form a laminate. FIG. 7 is a schematic view illustrating an example of a first lamination method. FIG. 8 is a schematic view illustrating an example of a second lamination method. FIG. 9 is a schematic view illustrating an example of a third lamination method. FIG. 10 is a schematic view illustrating an example of a fourth lamination method. FIG. 11 is a cross-sectional view showing an example of a state in which the aluminum base is dissolved and peeled from the laminate. FIG. 12 is an SEM (scanning electron microscope) image of a cross section of a porous carrier according to Example 5. Fig. 13 is an SEM image of the surface of the porous carrier according to Example 5. Fig. 14 is a graph showing the relationship between pore size and differential pore surface area for Example 5 and a reference example. Fig. 15 is a graph showing the relationship between pore size and cumulative pore surface area for Example 5 and a reference example. Fig. 16 is a plan view schematically showing the state before a bending test is performed. Fig. 17 is a side view schematically showing the state of an analytical carrier bent in a bending test. Fig. 18 is a plan view schematically showing the state of a test strip used in the examples. Fig. 19 is a photograph showing the results of a gold colloid test for Example 5 and a reference example.

[0013] The analytical carrier, immunochromatographic test strip, and analytical carrier manufacturing method according to the present embodiment will be described in detail below with reference to the drawings. The present disclosure is not limited to the following embodiments. Furthermore, some or all of the components in the embodiments can be combined as appropriate. The dimensional proportions in the drawings are exaggerated for the sake of explanation and may differ from the actual proportions.

[0014] [1. Analytical Carrier] First, an analytical carrier 1 according to this embodiment will be described with reference to FIG. 1. FIG. 1 is a schematic cross-sectional view showing an example of the analytical carrier 1. As shown in FIG. 1, the analytical carrier 1 includes a porous body 10 and a support 20 supporting the porous body 10 on one side. Typically, when the porous body 10 is dry, the difference between the refractive index of the aluminum oxide constituting the porous body 10 and that of air is large. Therefore, light incident on the porous body 10 is repeatedly refracted between the porous body 10 and the air layer. This increases the proportion of light reflected by the porous body 10, and reduces the amount of light that reaches the support 20, making the porous body 10 appear white. On the other hand, when the porous body 10 absorbs water, the refractive index of water is closer to that of aluminum oxide than that of air. This reduces the amount of light that reaches the support 20. Therefore, when the support 20 is an aluminum base material and the porous body 10 absorbs water, the analytical support 1 appears gray due to the gray color of the support 20. Therefore, in the analytical support 1 according to this embodiment, the porous body 10 is supported by the support 20, and when the porous body 10 absorbs water, the L of the analytical support 1 ＊ It was found that the ability to distinguish water absorbency can be improved by setting the value to 80 or more. The analytical carrier 1 according to this embodiment will be described in detail below.

[0015] <Porous body> The porous body 10 includes a skeleton 11 and a plurality of voids 12 surrounded by the skeleton 11. The skeleton 11 is formed by an aggregation of a plurality of hollow particles 13. The voids 12 are formed by the internal space of the porous body 10 surrounded by the skeleton 11 or a plurality of hollow particles 13. The hollow particles 13 have an outer shell 14 and a cavity 15 surrounded by the outer shell 14. The skeleton 11 includes the outer shell 14, and the surface of the skeleton 11 is formed by the outer shell 14. The skeleton 11 is formed by the continuous outer shells 14 of the plurality of hollow particles 13. The hollow particles 13 are connected via the outer shells 14 to form the skeleton 11 with a three-dimensional network structure. The outer shells 14 are arranged on the outer surface side of the porous body 10.

[0016] The shell 14 of the hollow particle 13 may have a through-hole 16 penetrating the shell 14 in the thickness direction. The through-hole 16 may connect the cavity 15 inside the hollow particle 13 separated by the shell 14 to the void 12 outside the hollow particle 13. The through-hole 16 may also connect the cavities 15 of adjacent hollow particles 13 to each other. The cavities 15 can be in communication with the outside of the hollow particle 13 or the skeleton 11 through the through-hole 16. When the porous body 10 is impregnated with a liquid containing a specimen or the like, the liquid can flow into or out of the cavities 15 through the through-hole 16 in the shell 14. Furthermore, the cavities 15 contained in adjacent hollow particles 13 are in communication. Therefore, when the porous body 10 is impregnated with a liquid, the liquid can flow through the cavity 15 via the through-hole 16 and into the skeleton 11.

[0017] Here, in immunochromatography, as will be described later, labels such as colored particles, gold colloid particles, or fluorescent beads are typically used. When the analytical carrier 1 is used, for example, in an immunochromatography test strip, the porous body 10 can absorb a solution in which the label is dispersed by capillary action. The mechanism by which this occurs is unclear, but it is presumed that the solution in which the label is dispersed penetrates the voids 12 and cavities 15 present inside the porous body 10, and that a portion of the solution flows along the surface of the porous body 10. Furthermore, while absorption is primarily achieved by the solution flowing through the voids 12, it is presumed that the flow of the solution through the cavities 15 and through-holes 16 also contributes to absorption.

[0018] In this way, the porous body 10 is a porous body having voids 12 therein that communicate with the outside. In this case, the voids 12 are surrounded by the outer shell 14. That is, the voids 12 are formed by being surrounded by the skeleton 11 inside the porous body 10 or the outer shell 14 formed on its surface. The voids 12 forming one cell structure surrounded by the outer shell 14 may be in communication with the voids 12 forming another cell structure. Specifically, the porous body 10 may have an open-cell structure. Furthermore, the single void 12 or the multiple voids 12 may or may not penetrate from one surface of the porous body 10 to the other surface.

[0019] The average particle diameter of the plurality of hollow particles 13 is 0.1 μm to 20 μm. By setting the average particle diameter to 0.1 μm or more, the strength of the porous body 10 is improved, and breakage during transportation, etc. can be suppressed. By setting the average particle diameter to 20 μm or less, the flow rate of the analytical carrier 1 can be improved. The average particle diameter may be 1 μm or more, 3 μm or more, or 5 μm or more. The average particle diameter may be 18 μm or less, 15 μm or less, or 10 μm or less.

[0020] The average particle size of the plurality of hollow particles 13 can be measured by observing the cross section of the porous body 10 with a scanning electron microscope. For example, after sintering, aluminum metal particles are partially melted and connected, but the portions having a substantially circular shape can be considered approximately circular. Therefore, in the above cross-sectional observation, the maximum diameter (longest diameter) of each of the substantially circular aluminum metal particles is defined as the particle size, and the particle sizes of 50 randomly selected aluminum metal particles are measured. The arithmetic average of these particles is defined as the average particle size of the sintered aluminum metal particles. The average particle size of the plurality of aluminum metal particles before sintering is the D50 value obtained by measuring the particle size distribution on a volume basis using a laser diffraction method. Note that if the particle size is within the range of 0.1 μm to 20 μm, almost no secondary particles of the aluminum metal particles are formed. Therefore, the average particle size of the sintered hollow particles 13 observed with a scanning electron microscope is substantially the same as the average particle size of the pre-sintered aluminum metal particles measured with a laser diffraction method.

[0021] The shape of each hollow particle 13 is not particularly limited and may be spherical, polygonal, irregular, scale-like, fibrous, or the like. Among these, a spherical shape is preferred for the hollow particles 13. When the hollow particles 13 are spherical, the sizes of the multiple voids 12 are uniform. For example, in immunochromatography, as described below, labels such as colored particles, gold colloid particles, or fluorescent beads are used. Furthermore, when the sizes of the voids 12 are uniform, such labels are not trapped within the voids 12 and can flow smoothly through the porous body 10. Note that the term "spherical" as used herein does not only refer to perfect spheres, but also to particles with slight surface irregularities. Furthermore, the term "spherical" as used herein does not only refer to hollow particles 13 with an aspect ratio of 1, but also includes hollow particles 13 with an aspect ratio of 1 or more. The aspect ratio of the hollow particles 13 may be 5 or less, 3 or less, or 2 or less. The aspect ratio is the ratio of the major axis to the minor axis of the hollow particles 13 and is the average value of multiple hollow particles 13.

[0022] The outer shell 14 includes an anodized film containing aluminum oxide. The anodized film included in the outer shell 14 may be a barrier-type anodized film or a porous-type anodized film. The outer shell 14 may be formed of only a barrier-type anodized film, or may be formed of a two-layer anodized film in which a porous-type anodized film is formed on the outer surface of the barrier-type anodized film. The outer shell 14 may have a hydrated film containing aluminum hydroxide. The hydrated film is formed in a hydration treatment process described below.

[0023] The ratio of metallic aluminum to the components constituting the porous body 10 containing aluminum element contained therein may be 0 or more and 0.1 or less in mass ratio. In other words, the ratio of the mass of metallic aluminum contained in the porous body 10 to the mass of the components constituting the porous body 10 containing aluminum element contained therein may be 0 or more and 0.1 or less in mass ratio. The mass of the components constituting the porous body 10 containing aluminum element refers to the total mass of aluminum oxide, aluminum hydroxide, electrolyte anions, resin residue (burnt residue), and metallic aluminum contained in the porous body 10. By setting the metallic aluminum ratio to 0.1 or less, the light transmittance of the porous body 10 is increased even when the porous body 10 has absorbed water, thereby reducing the gray color caused by the aluminum metal. This improves the distinguishability of water absorption. The metallic aluminum ratio is preferably 0.05 or less, more preferably 0.01 or less, and even more preferably 0.001 or less. The ratio of metallic aluminum to the components constituting the porous body 10 containing aluminum element contained in the porous body 10 can be calculated based on JIS G2404: 2022. The outer shell 14 of the hollow particle 13 may be composed essentially of an anodized film containing aluminum oxide.

[0024] The thickness of the outer shell 14 is preferably 40 nm to 1000 nm. By making the thickness of the outer shell 14 40 nm or more, damage to the porous body 10 can be suppressed even when the analytical carrier 1 is bent. This makes it easier to prevent the outer shell 14 from being broken and leaving wrinkles when the analytical carrier 1 is subjected to bending deformation. Furthermore, by setting the thickness of the outer shell 14 within this range, an analytical carrier 1 with sufficiently high corrosion resistance can be provided. The thickness of the outer shell 14 may be 100 nm or more, 200 nm or more, or 400 nm or more. The thickness of the outer shell 14 may be 900 nm or less, 700 nm or less, or 500 nm or less. When the thickness of the outer shell 14 is equal to or less than the above upper limit, when the outer shell is formed by alternately repeating the anodizing step and the dissolution step, the aluminum portion of the metal can be dissolved to form an outer shell of the desired thickness without forming an excessively thick anodized film, which makes it easier to improve production efficiency. The thickness of the outer shell 14 can be measured, for example, by observing a cross section of the outer shell 14 with a scanning electron microscope or the like.

[0025] The cumulative pore surface area of ​​the porous body 10 having a pore diameter of 0.1 μm or more and 100 μm or less is 0.1 m 2 / cm 3 More than 20m 2 / cm 3 The porous body 10 may have an integrated pore surface area of ​​0.1 m or less. 2 / cm 3 When the cumulative pore surface area of ​​the porous body 10 is 20 m or more, the amount of antibody adsorbed to the porous body 10 increases, thereby improving the absorbance of the analytical carrier 1. 2 / cm 3 When the cumulative pore surface area is 0.5 m or less, the water absorption of the porous body 10 can be improved. 2 / cm 3 It may be 1 m or more. 2 / cm 3 The cumulative pore surface area may be 10 m or more. 2 / cm 3 It may be less than 6m 2 / cm 3The cumulative pore surface area of ​​the porous body 10 can be obtained by converting the pore volumes of pores having a pore diameter of 0.1 μm or more and 100 μm or less, which are measured by mercury intrusion porosimetry, into surface areas for each pore diameter and then integrating the converted values.

[0026] The porosity of the porous body 10 is 50 vol% or more and less than 100 vol%. By setting the porosity of the porous body 10 to 50 vol% or more, the water absorption performance can be improved. By setting the porosity to less than 100 vol%, peeling of the hollow particles 13 from the porous body 10 can be suppressed, and the strength of the porous body 10 can be improved. The porosity may be 60 vol% or more, 70 vol% or more, 80 vol% or more, 90 vol% or more, or 95 vol% or more. The porosity may also be 95 vol% or less, 90 vol% or less, or 80 vol% or less. The porosity of the porous body 10 can be obtained by dividing the cumulative pore volume of the porous body 10 by the volume of the porous body 10. The cumulative pore volume of the porous body 10 can be obtained by integrating the pore volumes of pores having pore diameters of 0.1 μm or more and 100 μm or less obtained by mercury intrusion porosimetry.

[0027] The average pore diameter of the porous body 10 is 0.1 μm or more and 20 μm or less. By setting the average pore diameter to 0.1 μm or more, the water wicking performance of the analytical carrier 1 can be improved. Here, the particle diameter of typical labels used in immunochromatography, such as colored particles, gold colloid particles, or fluorescent beads, is approximately 10 nm to 50 nm, and labels with larger particle diameters also exist. When the analytical carrier 1 is used, for example, in an immunochromatographic test strip, setting the average pore diameter to 0.1 μm or more prevents such labels from being trapped in the voids 12, allowing the labels to flow smoothly through the porous body 10. Furthermore, by setting the average pore diameter to 20 μm or less, the water wicking performance of the analytical carrier 1 can be improved. The average pore diameter may be 0.5 μm or more, 1 μm or more, 3 μm or more, or 5 μm or more. The average pore diameter may be 15 μm or less, 12 μm or less, 10 μm or less, or 9 μm or less. The average pore diameter of the porous body 10 can be calculated using the following formula: Average pore diameter of the porous body 10 = 4 × (integral pore volume of the porous body 10) / (integral pore surface area of ​​the porous body 10). The integral pore volume and the integral pore surface area of ​​the porous body 10 can be obtained as described above.

[0028] The ratio of the integral pore surface area of ​​pores having a pore diameter of 0.1 μm or more and less than 1 μm to the integral pore surface area of ​​pores having a pore diameter of 1 μm or more and 10 μm or less may be 0.1 or more and 10 or less. The ratio of the integral pore surface areas may be 0.2 or more, 0.5 or more, or 1 or more. The ratio of the integral pore surface areas may be 8 or less, 6 or less, or 5 or less. The integral pore surface area of ​​pores having a pore diameter of 1 μm or more and 10 μm or less can be obtained by integrating the pore surface areas of pores having a pore diameter of 1 μm or more and 10 μm or less. Similarly, the integral pore surface area of ​​pores having a pore diameter of 0.1 μm or more and less than 1 μm can be obtained by integrating the pore surface areas of pores having a pore diameter of 0.1 μm or more and less than 1 μm. The integral pore surface area of ​​the porous body 10 can be determined by mercury intrusion porosimetry as described above. Signals such as absorbance from the analytical carrier 1 tend to be proportional to the flow rate. Furthermore, to lower the flow rate, in other words, to increase the water absorption rate, it is necessary to increase the porosity. In other words, there is a trade-off relationship in which an analytical carrier 1 with a high porosity and a fast water absorption rate produces a lower signal. When the cumulative pore surface area ratio is equal to or greater than the lower limit, the surface area of ​​the porous body 10 increases, increasing the amount of antibody bound to the porous body 10 and resulting in a stronger signal from the analytical carrier 1. Even if the analytical carrier 1 has a flow rate similar to that of a nitrocellulose membrane, a higher absorbance than a nitrocellulose membrane can be obtained by setting the cumulative pore surface area ratio equal to or greater than the lower limit. Setting the cumulative pore surface area ratio to the upper limit or less can prevent clogging of labeled particles.

[0029] The thickness of the porous body 10 is preferably 20 μm or more and 1 mm or less. By making the thickness of the porous body 10 20 μm or more, it is easy to ensure a sufficient thickness for absorbing water by capillary action. By making the thickness of the porous body 10 1 mm or less, it is possible to prevent the analytical carrier 1 from appearing gray when the porous body 10 absorbs water. The thickness of the porous body 10 may be 190 μm or less. By making the thickness of the porous body 10 190 μm or less, it is possible to prevent the porous body 10 from cracking and forming cracks when the analytical carrier 1 is bent, thereby preventing wrinkles from remaining. The thickness of the porous body 10 may be 30 μm or more, 40 μm or more, or 50 μm or more. The thickness of the porous body 10 may be 150 μm or less, or 100 μm or less.

[0030] The porous body 10 may be composed of a single porous layer, or may include multiple porous layers. The porous body 10 may include, for example, a first porous layer and a second porous layer disposed on the surface of the first porous layer. The first porous layer and the second porous layer may differ in any of the average particle size, aspect ratio, or constituent material of the multiple hollow particles 13, the porosity of the porous body 10, or the average pore size. For example, the average particle size of the hollow particles 13 contained in the first porous layer may be larger than the average particle size of the hollow particles 13 contained in the second porous layer. Furthermore, the thicknesses of the first porous layer and the second porous layer may be the same or different.

[0031] The porous body 10 may include a coating layer 17, which is provided on the surface opposite the support 20 and separates the inside of the porous body 10, where the skeleton 11 and voids 12 are present, from the outside of the porous body 10, and which includes an anodized film containing aluminum oxide. The coating layer 17 may have communicating holes 18 that connect the inside and outside of the porous body 10. The coating layer 17 may be formed continuously with the outer shells 14 of the hollow particles 13. The cavities 15 of the hollow particles 13 may be connected to the outside of the porous body 10 through the communicating holes 18. The inclusion of the coating layer 17 in the porous body 10 prevents the hollow particles 13 from being exposed to the outside, preventing direct contact with the porous body 10 by fingers or the like. This prevents the hollow particles 13 from peeling off. This improves the handleability of the analytical carrier 1.

[0032] The surface roughness Sa of the porous body 10 may be 0.01 μm or more and less than 1.8 μm. When the surface roughness Sa of the porous body 10 is less than 1.8 μm, the hollow particles 13 are not exposed on the surface of the porous body 10, and the surface of the porous body 10 tends to be covered by the coating layer 17. This makes it difficult for the hollow particles 13 of the porous body 10 to peel off from the porous body 10, the support 20, or the analytical carrier 1. The surface roughness Sa of the porous body 10 may be 0.1 μm or more, 0.2 μm or more, 0.4 μm or more, 0.6 μm or more, or 0.8 μm or more. The surface roughness Sa of the porous body 10 may be 1.5 μm or less, 1.2 μm or less, or 1 μm or less. The arithmetic mean roughness Sa can be obtained by measuring the surface of the porous body 10 opposite the support 20 in accordance with ISO 25178.

[0033] <Support> The support 20 supports the porous body 10 on one side. By supporting the porous body 10 with the support 20, it is possible to prevent the porous body 10 from being damaged due to deformation or the like. The support 20 may include an adhesive layer 22 that adheres to the porous body 10. The adhesive layer 22 may be adhered to the outer shell 14 of the porous body 10.

[0034] The support 20 may include an adhesive layer 22 that adheres to the porous body 10, and a support layer 21 that supports the adhesive layer 22. In this case, the adhesive layer 22 is interposed between the porous body 10 and the support layer 21. By adhering the porous body 10 and the support layer 21 with the adhesive layer 22, the porous body 10 and the support layer 21 can be firmly bonded together. Alternatively, the support 20 may not include the support layer 21, and may be constituted by the adhesive layer 22.

[0035] The adhesive layer 22 has adhesive sections 23 in which the material constituting the adhesive layer 22 penetrates the voids 12 and cavities 15 of the porous body 10, comes into contact with the hollow particles 13 and the outer shells 14, and adheres to the hollow particles 13 and the outer shells 14. The adhesive sections 23 are portions in which the material constituting the adhesive layer 22 and the hollow particles 13 and the outer shells 14 are mixed along the adhesive interface direction between the porous body 10 and the adhesive layer 22.

[0036] The adhesive layer 22 may have an adhesive portion 23 and an auxiliary portion 24. The auxiliary portion 24 is made of the material that constitutes the adhesive layer 22 and is continuous with the adhesive portion 23. It supports adhesion by lining the adhesive portion 23 from the side opposite the porous body 10. The auxiliary portion 24 is a portion formed by the material that constitutes the adhesive layer 22 along the adhesive interface direction between the porous body 10 and the adhesive layer 22. The auxiliary portion 24 is provided on the adhesive layer 22 on the side opposite the adhesive portion 23 that is in contact with the porous body 10. The auxiliary portion 24 is disposed between the adhesive portion 23 and the support layer 21, and by bonding the auxiliary portion 24 to the support layer 21, it also functions as a layer that bonds the adhesive portion 23 to the support layer 21. The auxiliary portion 24 also functions as a layer that bonds the adhesive layer 22 to the support layer 21.

[0037] For example, when a thermoplastic resin is used as the adhesive layer 22 to bond the adhesive layer 22 and the porous body 10, when the heated adhesive layer 22 comes into contact with the porous body 10, the softened thermoplastic resin in contact with the porous body 10 deforms and enters the voids 12 and cavities 15 of the porous body 10, and hardens as the temperature drops. At this time, the thermoplastic resin enters the voids 12 and cavities 15 of the porous body 10, resulting in a layer in which the thermoplastic resin, hollow particles 13, and shells 14 are mixed and in contact with each other, forming an adhesive portion 23. On the other hand, when the adhesive layer 22 and the porous body 10 are bonded, the portion of the thermoplastic resin that does not come into contact with the porous body 10 and remains a layer of thermoplastic resin, and is continuous with the adhesive portion 23, is called the auxiliary portion 24.

[0038] Furthermore, for example, when an adhesive (pressure-sensitive adhesive) is used as the adhesive layer 22 to bond the adhesive layer 22 and the porous body, when the adhesive layer 22 comes into contact with the porous body 10, the portion of the adhesive that comes into contact with the porous body 10 deforms and enters the voids 12 and cavities 15 of the porous body 10. At this time, as the adhesive enters the voids 12 and cavities 15 of the porous body 10, the layer portion where the adhesive, the hollow particles 13, and the shells 14 are mixed and in contact is called the adhesive portion 23. On the other hand, when the adhesive layer 22 and the porous body 10 are bonded, the portion of the adhesive that does not come into contact with the porous body 10, where the adhesive layer is maintained, and which is continuous with the adhesive portion 23 is called the auxiliary portion 24.

[0039] The thickness of the adhesive layer 22 may be 1 μm or more, 5 μm or more, 10 μm or more, 20 μm or more, or 30 μm or more. The thickness of the adhesive layer 22 may also be 1000 μm or less, 500 μm or less, 300 μm or less, 200 μm or less, 100 μm or less, 50 μm or less, or 40 μm or less. The thickness of the adhesive layer 22 is typically greater than the thickness of the adhesive portion 23. When the thickness of the adhesive layer 22 is equal to or greater than the above-described lower limit, the adhesive layer 22 adheres to the porous body 10, and when the support 20 is deformed, the adhesive layer 22 alleviates the deformation, thereby suppressing strain generated between the support 20 and the porous body 10, thereby making it easier to maintain the adhesion between the support 20 and the porous body 10. This makes it easier to prevent the porous body 10 from peeling off from the support 20 when the support 20 is deformed. Furthermore, it makes it easier to prevent wrinkles from occurring in the porous body 10 when the support 20 is deformed. When the thickness of the adhesive layer 22 is equal to or less than the upper limit, the adhesive layer 22 can be easily transported due to the appropriate bending during roll-to-roll production. The thickness of the adhesive layer 22 refers to the overall thickness in the thickness direction of the material constituting the adhesive layer 22 when the cross section is observed in a plane perpendicular to the adhesive interface direction between the porous body 10 and the adhesive layer 22.

[0040] The thickness of the adhesive portion 23 may be 1 μm or more, 2 μm or more, 3 μm or more, 4 μm or more, 5 μm or more, or 10 μm or more. The thickness of the adhesive portion 23 may also be 100 μm or less, 50 μm or less, 40 μm or less, 30 μm or less, 20 μm or less, or 10 μm or less. When the thickness of the adhesive portion 23 is equal to or greater than the above-mentioned lower limit, the adhesive portion 23 contacts the outer shell 14 with a sufficient thickness that is equal to or greater than the thickness of the outer shell 14. In this case, the adhesive portion 23 penetrates deep into the outer shell 14, thereby exerting an anchoring effect and facilitating strong bonding between the porous body 10 and the support 20. Furthermore, the increased contact area between the outer shell 14 and the adhesive portion 23 strengthens the effects of hydrogen bonding, van der Waals forces, ionic bonding, or covalent bonding by the adhesive portion 23, thereby facilitating strong bonding between the porous body 10 and the support 20. This makes it easier to prevent the porous body 10 from peeling off from the support 20 when the support 20 is deformed. Furthermore, it makes it easier to prevent wrinkles from occurring in the porous body 10 when the support 20 is deformed. Having the thickness of the adhesive portion 23 equal to or less than the above upper limit value makes it possible to prevent the porous body 10 from being completely filled with resin. The thickness of the adhesive portion 23 refers to the distance between a line passing through the portion of the porous body 10 closest to the adhesive layer 22 (the bottom) and parallel to the adhesive interface direction between the porous body 10 and the adhesive layer 22, and a line passing through the portion of the adhesive layer 22 closest to the porous body 10 (the top) and parallel to the adhesive interface direction between the porous body 10 and the adhesive layer 22, in the range where the material constituting the adhesive layer 22 is in contact with the porous body 10, when observed in cross section in a plane perpendicular to the adhesive interface direction between the porous body 10 and the adhesive layer 22.

[0041] The thickness of the support layer 21 may be thicker than the thickness of the adhesive layer 22. The thickness of the support layer 21 may be 10 μm or more, 50 μm or more, 70 μm or more, 90 μm or more, or 100 μm or more. The thickness of the support layer 21 may be 1000 μm or less, 500 μm or less, 300 μm or less, 200 μm or less, 150 μm or less, or 100 μm or less.

[0042] The support 20 may be flexible. When a porous body is disposed on the surface of an aluminum substrate, such as the aluminum member described in Patent Document 1, bending the analytical carrier 1 may cause the aluminum substrate to plastically deform and not return to its original shape. In this case, the porous body disposed on the surface of the aluminum substrate may also wrinkle in response to the aluminum substrate. On the other hand, when the analytical carrier 1 is flexible, the analytical carrier 1 is more likely to return to its original shape even after bending deformation, making the support 20 less likely to wrinkle. The support 20 supports the porous body 10, and wrinkles are less likely to form on the support 20, which can prevent wrinkles from forming on the porous body 10 of the analytical carrier 1.

[0043] The adhesive layer 22 may contain at least one selected from the group consisting of resin, elastomer, starch, and protein. That is, the adhesive layer 22 may be a resin adhesive layer or an elastomer adhesive layer. The resin may contain at least one selected from the group consisting of a thermoplastic resin, a thermosetting resin, and a UV-curable resin. The UV-curable resin may contain at least one of a radical-curable resin and a cation-curable resin. The resin may contain at least one selected from the group consisting of polyolefins such as polyethylene (HDPE, MDPE, LDPE), polypropylene, modified polyolefin resins (modified polypropylene, modified polyethylene, etc.), ethylene-vinyl acetate copolymers, polyvinyl chloride, polyvinyl acetate, polyamide, polyester, (meth)acrylic resin, epoxy resin, urethane resin, fluororesin, styrene resin, ABS resin, silicone resin, phenolic resin, melamine resin, polyvinyl alcohol, and polycyanoacrylate. The resin may include adhesives (pressure-sensitive adhesives) such as isocyanate-based adhesives, urethane resin-based adhesives, urethane resin solvent-based adhesives, urethane resin emulsion adhesives, acrylic resin-based adhesives, anaerobic acrylic resin adhesives, olefin-based adhesives, silicone-based adhesives, rubber-based adhesives, ethylene vinyl acetate resin emulsion adhesives, epoxy resin-based adhesives, epoxy resin emulsion adhesives, vinyl chloride resin solvent-based adhesives, chloroprene rubber-based adhesives, cyanoacrylate-based adhesives, aqueous polymer-isocyanate-based adhesives, styrene-butadiene rubber solution-based adhesives, styrene-butadiene rubber latex adhesives, nitrile rubber-based adhesives, nitrocellulose adhesives, phenolic resin-based adhesives, modified silicone-based adhesives, polyimide-based adhesives, polystyrene resin solvent-based adhesives, polybenzimidazole adhesives, polymethacrylate resin solution-based adhesives, and photo- or UV-curable resin-based adhesives. The elastomer may include at least one selected from the group consisting of thermoplastic elastomers and rubbers. The adhesive layer 22 may include an ethylene vinyl acetate copolymer. Ethylene vinyl acetate copolymer melts at a relatively low temperature, so that the porous body 10 and the support layer 21 can be easily bonded together.When the adhesive layer 22 contains a thermoplastic resin, the melting point of the adhesive layer 22 is preferably lower than the melting point of the support 20. From the viewpoint of suppressing deformation of the support 20 when the analytical carrier 1 is subjected to bending deformation, the adhesive layer 22 preferably contains a highly flexible material.

[0044] The support layer 21 may contain at least one of resin and glass. These materials can effectively support the porous body 10. The L measured when the analytical carrier 1 with the porous body 10 absorbed water is placed on a white reflection standard. ＊ From the viewpoint of improving the value, the support layer 21 preferably contains a material with high light transmittance or a material with high whiteness. Furthermore, from the viewpoint of suppressing deformation of the support 20 when the analytical carrier 1 is subjected to bending deformation, the support layer 21 preferably contains a material that is resistant to plastic deformation when a certain level of strain is applied. The resin may contain at least one selected from the group consisting of a thermoplastic resin, a thermosetting resin, and a UV-curable resin. The resin may contain at least one selected from the group consisting of polyester, acrylic resin, urethane resin, silicone resin, epoxy resin, polyolefin, cellulose resin, styrene resin, polyimide, and polycarbonate.

[0045] The support layer 21 may be a single layer or may be multi-layered. When the support layer 21 is multi-layered, the materials of the layers may be the same or different. When the support layer 21 is multi-layered, it is preferable that the melting point of the material of the support layer 21 arranged closest to the adhesive layer 22 is lower than the melting point of the material of the support layer 21 arranged closest to the adhesive layer 22. The support layer 21 may be, for example, a two-layered layer of LDPE (low-density polyethylene) and PET (polyethylene terephthalate). Such an arrangement can prevent the adhesive layer 22 from protruding during lamination.

[0046] The resin contained in the support layer 21 may contain a filler. The filler may contain at least one of an organic filler and an inorganic filler. The organic filler may contain at least one selected from the group consisting of a resin filler, a cellulose nanofiber, and a pigment. The inorganic filler may contain at least one selected from the group consisting of an oxide, a hydroxide, a carbide, a nitride, a boride, a silicide, and a fluoride. The filler may be spherical, polygonal, irregular, scaly, needle-like, or fibrous. The support layer 21 may be in the form of a plate, a sheet, a woven fabric, or a nonwoven fabric.

[0047] <Analytical Carrier> The analytical carrier 1 includes a porous body 10 and a support 20. The support 20 may include an adhesive layer 22. The support 20 may include an adhesive layer 22 and a support layer 21. The adhesive layer 22 of the support 20 may be bonded to the outer shell 14 of the porous body 10. The adhesive layer 22 may be bonded to the outer shell 14, and the porous body 10 and the support 20 may be laminated by bonding together with the adhesive layer 22 interposed between the porous body 10 and the support layer 21. The adhesive layer 22 may include an adhesive portion 23 and an auxiliary portion 24. The support 20 may include the adhesive layer 22 including the adhesive portion 23 and the auxiliary portion 24, and the support layer 21, and the adhesive portion 23, auxiliary portion 24, and support layer 21 may be laminated together in this order. Since the adhesive portion 23 and the outer shell 14 are bonded together and the auxiliary portion 24 and the support layer 21 are bonded together, the porous body 10, the adhesive portion 23, the auxiliary portion 24, and the support layer 21 may be stacked in this order.

[0048] The adhesive layer 22 may be bonded to the shell 14 located on the surface of the porous body 10 opposite the coating layer 17. The porous body 10 may have the shell 14 bonded to the adhesive layer 22, and the coating layer 17 located on the surface opposite the shell 14 bonded to the adhesive layer 22 exposed. In the analytical carrier 1, when the porous body 10 and the support 20 are bonded together, the coating layer 17 may be provided on the opposite side of the support 20 and located outside the porous body 10, and the hollow particles 13 and the shell 14 may be located inside the porous body 10 sandwiched between the coating layer 17 and the support 20. In this way, in the analytical carrier 1, the coating layer 17 is located on the outside of the porous body 10, and the hollow particles 13 and the shell 14 are located inside the porous body 10, and the hollow particles 13 and the shell 14 are covered by the coating layer 17 provided on the surface layer of the analytical carrier 1. This prevents the hollow particles 13 from peeling off from the porous body 10, the support 20, or the analytical carrier 1, making it easier to handle.

[0049] The thickness of the analytical carrier 1 may vary depending on the application, but may be, for example, 20 μm or more and 10 cm or less. By setting the thickness of the analytical carrier 1 within this range, an analytical carrier 1 with good bending strength can be provided. The thickness of the analytical carrier 1 may be 30 μm or more, 50 μm or more, 100 μm or more, or 150 μm or more. The thickness of the analytical carrier 1 may be 1000 μm or less, 300 μm or less, or 200 μm or less.

[0050] The porous body 10 is provided on a first surface, which is one surface of the support 20, and the second surface, which is the surface opposite to the first surface, may be exposed. With this configuration, the analytical carrier 1 can improve problems caused by reattaching the porous body 10. Specifically, when preparing an immunochromatographic test strip or test kit, an adhesive member constituting the immunochromatographic test strip or test kit is attached to the support 20. However, when attempting to peel off the adhesive member attached to the support 20 from the support 20 and then reattach it for position adjustment or the like, it may be difficult to reattach it.

[0051] For example, when the porous body 10 is provided on both sides of an aluminum substrate (described later), if an attachment member is attached to the porous body 10 provided on one side of the aluminum substrate and then the attachment member is peeled off, the porous body 10 may peel off from the aluminum substrate. In such cases, the porous body 10 remains on the attachment member, which may weaken the adhesive strength of the attachment member. Furthermore, when the porous body 10 is provided on one side of the aluminum substrate, if the attachment member is attached to the side of the aluminum substrate where the porous body 10 is not provided and then the attachment member is peeled off, the aluminum substrate may undergo plastic deformation. In such cases, wrinkles may occur in the porous body 10.

[0052] On the other hand, when the porous body 10 is provided on the first surface of the support 20 and the second surface of the support 20 is exposed, the above-mentioned problems caused by re-attachment can be suppressed by attaching an attachment member to the support 20. The first surface and the second surface may be the main surfaces. Here, the main surfaces are surfaces perpendicular to the thickness direction of the support 20, and are the two surfaces with the first and second largest areas among the six surfaces forming the support 20.

[0053] The analytical carrier 1 with the porous body 10 absorbing water is placed on a white reflection standard with which the measuring instrument is calibrated, and the surface of the porous body 10 on the analytical carrier 1 is measured with the measuring instrument. ＊ a ＊ b ＊ L in color system ＊ The value is 80 or more. ＊ By making the value 80 or more, it is possible to improve the distinguishability when water is absorbed. ＊ The value may be 85 or more, 90 or more, or 95 or more. ＊ a ＊ b ＊ L in color system ＊The L value can be measured using a color difference meter with a 45° circular illumination and vertical light receiving method conforming to JIS Z8722. The analytical carrier 1 with water absorbed into the porous body 10 can be obtained by making the porous body 10 absorb pure water. The L value when a white reflection standard with which the measuring instrument is calibrated is measured with the measuring instrument is ＊ a ＊ b ＊ L in color system ＊ The value is 97.1.

[0054] The analytical carrier 1 with the dried porous body 10 is placed on a white reflection standard with which the measuring instrument is calibrated, and the surface of the porous body 10 on the analytical carrier 1 is measured with the measuring instrument. ＊ a ＊ b ＊ L in color system ＊ The value may be 80 or more. ＊ By setting the value to 80 or more, it is possible to improve the discrimination ability when water is absorbed. ＊ The value may be 85 or more, 90 or more, or 95 or more. Note that the porous body 10 being dry means that the porous body 10 has not absorbed any water.

[0055] It is preferable that the time required for the analytical carrier 1 to wick water up to a height of 4 cm by capillary action is 400 seconds or less. This makes it possible to provide an analytical carrier 1 suitable for, for example, chromatography. This time may be 20 seconds or more, 40 seconds or more, or 50 seconds or more. This time may also be 360 ​​seconds or less, 200 seconds or less, or 100 seconds or less. This time can be obtained, for example, by immersing the analytical carrier 1 in pure water at room temperature (30°C) so that the planar direction of the analytical carrier 1 is perpendicular to the liquid surface, and measuring the time required for water to wick up to a height of 4 cm by capillary action. The pure water has a resistivity of 10 kΩ-m measured at 30°C.

[0056] As shown in FIGS. 16 and 17 , it is preferable that the analytical carrier 1 does not experience peeling of the porous body 10 during the bending test. It is also preferable that the analytical carrier 1 does not leave wrinkles on the surface of the porous body 10 during the bending test. Specifically, in the bending test, a 10 g weight 230 is attached to each end of an analytical carrier 1 cut into a 10 mm x 100 mm piece. The analytical carrier 1 is then placed on a stainless steel rod 240 at the longitudinal center of the analytical carrier 1 so that the porous body 10 is in contact with the stainless steel rod 240. The analytical carrier 1 is then lifted until the weights 230 at both ends of the analytical carrier 1 float, and held for 10 seconds. After holding for 10 seconds, the stainless steel rod 240 is returned to its original position, and the surface of the porous body 10 is visually observed and evaluated. An analytical carrier 1 that does not experience peeling of the porous body 10 during the bending test is less likely to peel when the analytical carrier 1 is bent or reattached, making it easier to handle the analytical carrier 1. Furthermore, an analytical carrier 1 that does not leave wrinkles on the surface of the porous body 10 in a bending test is less likely to leave wrinkles when bent or reattached, making it easier to handle the analytical carrier 1. Peeling of the porous body 10 refers to separation of the adhesive layer 22 and the porous body 10, and refers to a situation in which the minimum width of the separated portion within the surface of the analytical carrier 1 is 2 mm or more and the maximum length is 5 mm or more. Wrinkles in the porous body 10 refer to wrinkles of millimeter order size that can be visually confirmed.

[0057] <Effects> As described above, the analytical carrier 1 according to this embodiment includes a porous body 10 and a support 20 that supports the porous body 10 on one side. The porous body 10 includes a skeleton 11 formed by an aggregation of a plurality of hollow particles 13, and a plurality of voids 12 surrounded by the skeleton 11. The hollow particles 13 have an outer shell 14 that includes an anodized film containing aluminum oxide, and a cavity 15 surrounded by the outer shell 14. The skeleton 11 is formed by the continuous outer shells 14 of the plurality of hollow particles 13. The porosity of the porous body 10 is 50% by volume or more and less than 100% by volume. The average pore diameter of the porous body 10 is 0.1 μm or more and 20 μm or less. The analytical carrier 1 with the porous body 10 absorbing water is placed on a white reflection standard with which the measuring instrument is calibrated, and the L＊ a ＊ b ＊ L in color system ＊ The value is 80 or greater.

[0058] The outer shell 14 including the anodized coating is highly transparent, and the porous body 10 becomes transparent when it absorbs water. In an aluminum member in which a porous body is supported by an aluminum base material, as in Patent Document 1, when the porous body absorbs water, the gray color of the aluminum substrate supporting the porous body can be seen through the porous body, causing the aluminum member to appear gray.

[0059] The analytical carrier 1 according to this embodiment includes a support 20 that supports the porous body 10. The analytical carrier 1 with the porous body 10 absorbing water is placed on a white reflection standard with which the measuring instrument is calibrated, and the L ＊ a ＊ b ＊ L in color system ＊ The value is set to 80 or more. Therefore, the analytical carrier 1 according to this embodiment can improve the distinguishability when absorbing water. For example, when a transparent resin is used as the support 20, the distinguishability of the analytical carrier 1 when absorbing water can be improved by evaluating the analytical carrier 1 on a white reflective standard.

[0060] [2. Immunochromatography Test Strip] The immunochromatography test strip includes an analytical carrier 1. The analytical carrier 1 can analyze the analyte while supporting the analyte. The analytical carrier 1 can improve discrimination upon water absorption, and therefore can be suitably used as an immunochromatography test strip. The immunochromatography test strip is also called an immunochromatography developing member, a lateral flow assay test strip, or a lateral flow assay developing member. The analytical carrier 1 is also preferably used in in vitro diagnostic pharmaceuticals such as test kits that use immunochromatography.

[0061] [3. Test Kit] Next, an example of a test kit 50 using the analytical carrier 1 will be described. The test kit 50 is also sometimes referred to as a diagnostic kit. As shown in FIG. 2 , the test kit 50 includes a test strip 60 having the analytical carrier 1. Specifically, the test strip 60 includes the analytical carrier 1, a specimen supply section 62, and an absorption section 64. The analytical carrier 1 is provided with a determination section 66. The test kit 50 may further include a case (not shown) for storing the test strip 60.

[0062] The specimen supply section 62 may contain, for example, a labeled antibody that specifically binds to an antigen, which is the analyte. A specimen collected from a living organism or the like is supplied to the specimen supply section 62 and mixed with the labeled antibody to form a liquid mixture. The liquid mixture is developed to the determination section 66 by capillary action of the analytical carrier 1, and excess specimen is absorbed by the absorption section 64.

[0063] The determination unit 66 has, for example, a test line and a control line. For example, a capture antibody that specifically binds to the analyte is immobilized on the test line. When the analyte is contained in the sample, a labeled antibody is immobilized to the capture antibody on the test line via the analyte. For example, an antibody that specifically binds to the labeled antibody is immobilized on the control line. When the mixture containing the sample and the labeled antibody is developed up to the control line, the labeled antibody binds to the antibody immobilized on the control line.

[0064] The labeled antibody contains a label containing labeled particles such as colored particles, colloidal gold particles, or fluorescent beads, and an antibody that binds to the label to form a complex and specifically binds to the analyte. Therefore, if there is a location with a high concentration or density of labeled antibody, the fluorescence of that location can be confirmed by the dense label. Therefore, with the test kit 50, a positive result is indicated when both the test line and the control line are visible, and a negative result is indicated when only the control line is visible. The test line and control line can be visually confirmed when colored particles or colloidal gold particles are used, and can be confirmed with a fluorescence detector such as a fluorescence scanner when fluorescent beads are used.

[0065] The fluorescence can be detected by a fluorescence detector such as a fluorescence scanner, which irradiates the analytical support 1 carrying the analyte with excitation light and detects the fluorescence of the fluorescent beads excited by the excitation light.

[0066] The test kit 50 can be used for, for example, infectious disease testing; genetic analysis; pregnancy testing; livestock testing; and allergen testing for food, animals, plants, metals, house dust, etc.

[0067] Examples of the analyte of the test kit 50 include amino acids, peptides, proteins, genes, sugars, lipids, cells, or complexes thereof. More specifically, the analyte may be a peptide such as PCT (procalcitonin), a protein such as urinary albumin, a hormone such as HCG (human chorionic gonadotropin) or LH (luteinizing hormone), or a viral infection such as HBs antigen, rotavirus antigen, adenovirus antigen, RSV (respiratory syncytial virus) antigen, influenza virus antigen, norovirus antigen, mumps virus antigen, cytomegalovirus antigen, herpes simplex virus antigen, varicella-zoster virus antigen, SARS (severe acute respiratory syndrome) antigen, HBs antibody, HCV (hepatitis C virus) antibody, HIV antibody, EBV antibody, RSV antibody, rubella virus antibody, measles virus antibody, enterovirus antibody, dengue virus antibody, or SARS antibody. antigens or antibodies of bacterial infections such as pneumococcal antigens, mycoplasma antigens, group A hemolytic streptococcus antigens, legionella antigens, mycobacterium tuberculosis antigens, gonococcus antigens, tetanus antigens, mycoplasma antibodies, Helicobacter pylori antibodies, and mycobacterium tuberculosis antibodies; antigens or antibodies of chlamydia infections such as chlamydia antigens; antigens or antibodies of spirochete infections such as Treponema pallidum antibodies; antigens or antibodies of protozoal diseases such as malaria antibodies and toxoplasma antibodies.

[0068] In addition, the analytical carrier 1 can also be used as, for example, a gas or liquid separation membrane; a moisture-absorbing material; a water-absorbing material; an adsorbent material for adsorbing foreign substances such as pollen, particulate matter, bacteria, odor components, and heavy metals; a wiping sheet; a test sheet for chemicals such as concentrated sulfuric acid, urinalysis, and pH testing; a disinfecting and sterilizing material; a reflective material (standard white board); a separator for batteries and electric double-layer capacitors; a catalyst carrier; a reaction field for synthesis reactions; and a heat-insulating material. Examples of the separation membrane include reverse osmosis membranes, ion exchange membranes, and gas separation membranes. Examples of the adsorbent material include masks, filtration membranes, and filters.

[0069] [4. Manufacturing Method of Analytical Support] Next, a manufacturing method of the analytical support 1 according to this embodiment will be described. The manufacturing method of the analytical support 1 according to this embodiment includes a sintering step, an anodizing step, a dissolving step, a laminating step, and a peeling step. Furthermore, the manufacturing method of the analytical support 1 may include a hydration treatment step, if necessary. Each step will be described in detail below.

[0070] <Sintering Step> The sintering step is a step of sintering a plurality of aluminum metal particles 113 on the aluminum base 105. This step makes it possible to obtain a sintered material 100 including the aluminum base 105 and a sintered body 110 laminated on the aluminum base 105 and in which the aluminum metal particles 113 are sintered, as shown in Fig. 3. Fig. 3 illustrates the sintered body 110 in which the aluminum metal particles 113 are sintered on one surface of the aluminum base 105, but it is sufficient that the aluminum metal particles 113 are sintered on at least one surface of the aluminum base 105, and the aluminum metal particles 113 may also be sintered on both surfaces of the aluminum base 105. In other words, the sintered body 110 may have the aluminum metal particles 113 on one surface of the aluminum base 105, or may have the aluminum metal particles 113 on both surfaces of the aluminum base 105.

[0071] The aluminum metal particles 113 contain at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and aluminum alloys. Hereinafter, the at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and aluminum alloys will also be simply referred to as aluminum.

[0072] The aluminum content of high-purity aluminum may be 99.95% by mass or more, 99.99% by mass or more, or 99.995% by mass or more. The aluminum content of pure aluminum may be 99.00% by mass or more, 99.50% by mass or more, or 99.80% by mass or more. The aluminum content of pure aluminum is less than 99.95% by mass. High-purity aluminum and pure aluminum may contain elements other than aluminum (Al). The elements contained in pure aluminum other than aluminum may include one or more of elements such as silicon (Si), iron (Fe), copper (Cu), manganese (Mn), magnesium (Mg), chromium (Cr), zinc (Zn), titanium (Ti), vanadium (V), gallium (Ga), nickel (Ni), boron (B), and zirconium (Zr). The pure aluminum may be an A1000 series alloy specified in JIS H4000.

[0073] The aluminum alloy contains aluminum and elements other than aluminum. The elements other than aluminum contained in the aluminum alloy may include one or more of silicon (Si), iron (Fe), copper (Cu), manganese (Mn), magnesium (Mg), chromium (Cr), zinc (Zn), titanium (Ti), vanadium (V), gallium (Ga), nickel (Ni), boron (B), zirconium (Zr), and the like. The total content of the elements other than aluminum contained in the aluminum alloy may be more than 1 mass%. The total content of the elements other than aluminum contained in the aluminum alloy may be 10 mass% or less, or may be 5 mass% or less. The content of each of the elements other than aluminum contained in the aluminum alloy may be 10 mass% or less, or may be 1 mass% or less. The aluminum alloy may be a 1000 series alloy, a 2000 series alloy, a 3000 series alloy, a 4000 series alloy, a 5000 series alloy, a 6000 series alloy, a 7000 series alloy, or an 8000 series alloy as specified in JIS H4000.

[0074] The average particle diameter of the aluminum metal particles 113 is 0.1 μm or more and 20 μm or less. By setting the average particle diameter of the aluminum metal particles 113 to 0.1 μm or more, the strength of the porous body 10 is improved, and breakage during transportation, etc. can be suppressed. By setting the average particle diameter of the aluminum metal particles 113 to 20 μm or less, the flow rate of the analytical carrier 1 can be improved. The average particle diameter of the aluminum metal particles 113 may be 1 μm or more, 3 μm or more, or 5 μm or more. The average particle diameter of the aluminum metal particles 113 may be 18 μm or less, 15 μm or less, or 10 μm or less. The average particle diameter of the aluminum metal particles 113 before sintering is the D50 value obtained by measuring the particle size distribution on a volume basis using a laser diffraction method. The shape of the particles is not particularly limited and may be spherical, polygonal, irregular, scaly, fibrous, or the like.

[0075] The aluminum metal particles 113 can be produced by a known method. The aluminum metal particles 113 can be produced by, for example, an atomization method, a melt spinning method, a rotating disk method, a rotating electrode method, or other rapid solidification methods. Among these, from the viewpoint of industrial productivity, the aluminum metal particles 113 are preferably produced by an atomization method, and more preferably by a gas atomization method. Specifically, the aluminum metal particles 113 are preferably produced by atomizing a molten metal.

[0076] The aluminum base 105 contains at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and aluminum alloys. The high-purity aluminum, pure aluminum, and aluminum alloys can be those described for the aluminum metal particles 113. The composition of the aluminum contained in the aluminum metal particles 113 and the composition of the aluminum contained in the aluminum base 105 may be the same as or different from each other.

[0077] The thickness of the aluminum base 105 is greater than 0 μm. The thickness of the aluminum base 105 may be 10 μm or more, or 20 μm or more, depending on the application. The thickness of the aluminum base 105 may be, for example, 1 mm or less, 100 μm or less, 10 μm or less, or 1 μm or less.

[0078] The plurality of aluminum metal particles 113 may be disposed on at least one surface of the aluminum substrate 105 and then sintered. On at least one surface of the aluminum substrate 105, aluminum powder containing the plurality of aluminum metal particles 113 may be disposed, a green compact obtained by compacting the aluminum powder may be disposed, or a liquid composition such as a slurry containing the plurality of aluminum metal particles 113 may be disposed. The liquid composition may be applied to the surface of the aluminum substrate 105 by a known method such as spray coating, brush coating, roller coating, air knife coating, bar coating, spin coating, dipping, or screen printing. The liquid composition may be applied to the surface of the aluminum substrate 105 to achieve the desired thickness of the porous body 10, taking into consideration the composition.

[0079] Before disposing the plurality of aluminum metal particles 113 on the surface of the aluminum substrate 105, the surface of the aluminum substrate 105 may be pretreated. The pretreatment may include a step of roughening the surface of the aluminum substrate 105. The pretreatment is not particularly limited, and may be cleaning, etching, blasting, or the like.

[0080] The aluminum powder or composition may contain, in addition to the plurality of aluminum metal particles 113, a pore-forming material, a binder, a sintering aid, a surfactant, a solvent, and the like, as necessary. Any of these may be known.

[0081] The content of aluminum metal particles 113 in the composition is preferably 20% by mass or more, more preferably 30% by mass or more, and even more preferably 31% by mass or more. The content of aluminum metal particles 113 in the composition is preferably 70% by mass or less, more preferably 60% by mass or less, and even more preferably 57% by mass or less.

[0082] The pore-forming material is a material that promotes the formation of voids 12 in the sintered body 110. The pore-forming material may be, for example, particles containing a polymer material. It is preferable that the pore-forming material has low solubility in the solvent described below. The polymer material may contain a polysaccharide or a resin. The polysaccharide may contain, for example, starch. The resin may contain a polyolefin such as polyethylene or polypropylene.

[0083] The average particle diameter of the pore-forming material is preferably 0.1 μm or more and 20 μm or less. By setting the average particle diameter of the pore-forming material to 0.1 μm or more, the average pore diameter can easily be set to 0.1 μm or more. By setting the average particle diameter of the pore-forming material to 20 μm or less, the average pore diameter can easily be set to 20 μm or less. The average particle diameter of the pore-forming material may be 0.5 μm or more, or may be 1 μm or more. Furthermore, the average particle diameter of the pore-forming material may be 10 μm or less, or may be 8 μm or less. The average particle diameter of the pore-forming material is the D50 value determined by measuring the particle size distribution on a volume basis by laser diffraction.

[0084] The content of the pore-forming material in the composition may be 5% by mass or more, 10% by mass or more, or 12% by mass or more. The content of the pore-forming material in the composition may be 30% by mass or less, 25% by mass or less, or 23% by mass or less. When the content of the pore-forming material is equal to or greater than the lower limit of the above range, the formation of voids 12 in the sintered body 110 is promoted, and the filling rate of the sintered body 110 is reduced, making it easier to obtain a sintered body 110 having a desired filling rate and a porous body 10 having a desired porosity. Furthermore, when the content of the pore-forming material is equal to or less than the upper limit of the above range, an excessive decrease in the filling rate of the sintered body 110 is prevented, making it easier to suppress a decrease in the strength of the porous body 10.

[0085] The binder preferably has high solubility in the solvent described below. The binder may contain, for example, a synthetic resin such as a carboxy-modified polyolefin resin, a vinyl acetate resin, a vinyl chloride resin, a vinyl chloride-vinyl acetate copolymer resin, a vinyl alcohol resin, a butyral resin, a vinyl fluoride resin, an acrylic resin, a polyester resin, a urethane resin, an epoxy resin, a urea resin, a phenolic resin, an acrylonitrile resin, a nitrocellulose resin, paraffin wax, or polyethylene wax, or a natural resin such as wax, tar, glue, urushi, pine resin, or beeswax. The content of the binder in the composition may be 0.5% by mass or more, or may be 1% by mass or more. The content of the binder in the composition may be 30% by mass or less, or may be 20% by mass or less.

[0086] The solvent may contain an organic solvent such as water, ethanol, toluene, ketones, esters, etc. To volatilize the solvent, the laminate obtained by applying the composition to the aluminum substrate 105 may be dried at a temperature of 20° C. or higher and 300° C. or lower for 1 minute to 30 minutes, if necessary.

[0087] The sintering temperature is not particularly limited, but is preferably 560°C or higher and 660°C or lower. By setting the sintering temperature to 560°C or higher, the strength of the porous body 10 can be improved. By setting the sintering temperature to 660°C or lower, melting of the aluminum metal particles 113 can be suppressed. The sintering temperature may be 570°C or higher, or 580°C or higher. Furthermore, the sintering temperature may be 650°C or lower, or 620°C or lower.

[0088] The sintering time depends on the sintering temperature, etc., but may be, for example, about 5 to 24 hours. The sintering atmosphere is not particularly limited and may be, for example, a vacuum atmosphere, an inert gas atmosphere, an oxidizing gas atmosphere (air), or a reducing gas atmosphere. Among these, the sintering atmosphere is preferably a vacuum atmosphere or a reducing gas atmosphere. Furthermore, the sintering conditions may be any pressure condition of normal pressure, reduced pressure, or increased pressure.

[0089] When the composition contains a pore-forming material, it is preferable to heat the composition at a temperature of 200°C or higher and 500°C or lower before sintering. By heating the composition at 200°C or higher, the pore-forming material is slowly burned, and more uniformly dispersed voids 12 can be formed in the sintered body 110. By heating the composition at 500°C or lower, oxidation of the surfaces of the aluminum metal particles 113 during heating can be suppressed, and the strength of the porous body 10 can be improved. The heating temperature may be 250°C or higher, or 280°C or higher. The heating temperature may be 460°C or lower, or 430°C or lower.

[0090] The heating time is preferably 5 hours or more and 20 hours or less. By setting the heating time to 5 hours or more, more uniformly dispersed voids 12 can be formed in the sintered body 110. By setting the heating time to 20 hours or less, sintering of the aluminum metal particles 113 can be prevented from progressing, and more uniformly dispersed voids 12 can be formed in the sintered body 110. The heating time may be 7 hours or more or 15 hours or less. The sintering atmosphere may be any of a vacuum atmosphere, an inert gas atmosphere, and an oxidizing gas atmosphere. Furthermore, the sintering conditions may be any of atmospheric pressure, reduced pressure, and increased pressure.

[0091] The sintering process may include a first sintered layer forming process for obtaining a first sintered layer and a second sintered layer forming process for obtaining a second sintered layer. In the first sintered layer forming process, a plurality of aluminum metal particles 113 may be sintered to obtain the first sintered layer. As described above, the plurality of aluminum metal particles 113 may be arranged on at least one surface of the aluminum base material 105 and then sintered. In the second sintered layer forming process, a laminate in which the plurality of aluminum metal particles 113 are arranged on the surface of the first sintered layer may be sintered. In the first sintered layer forming process and the second sintered layer forming process, at least one of the average particle size, aspect ratio, constituent material, composition of the composition, or type or average particle size of the pore-forming material used for the plurality of aluminum metal particles 113 may be different.

[0092] The filling rate of the sintered body 110 is 10% to 60% by volume. By setting the filling rate of the sintered body 110 to 10% by volume or more, it is possible to easily obtain a porous body 10 having a porosity equal to or less than the upper limit of the desired range after the anodizing process and the dissolving process. This prevents the aluminum metal particles 113 from peeling off from the porous body 10, thereby improving the strength of the porous body 10. Furthermore, by setting the filling rate of the sintered body 110 to 60% by volume or less, it is possible to easily obtain a porous body 10 having a porosity equal to or greater than the lower limit of the desired range after the anodizing process and the dissolving process. This improves the wicking performance of the porous body 10. The filling rate may be 15% by volume or more, or may be 20% by volume or more. Furthermore, the filling rate may be 55% by volume or less, or may be 50% by volume or less. As described in the Examples section below, the filling rate can be obtained by dividing the mass of the sintered body 110 obtained by subtracting the mass of the aluminum base material 105 from the mass of the entire sintered material 100 by the mass of the sintered body 110 when the filling rate is assumed to be 100%.

[0093] <Hydration Treatment Step> The method for manufacturing the analytical support 1 according to this embodiment may include a hydration treatment step prior to the anodization step. The hydration treatment step forms a hydrated film on the surface of the aluminum metal particles 113, converting the hydrated film into an oxide film during the subsequent anodization step, thereby efficiently forming the anodized film. The hydration treatment step also has the effect of lifting and cleaning contaminants present on the aluminum surface prior to anodization, thereby reducing the occurrence of uneven appearance when an anodized film is subsequently formed. The hydration treatment step is a step in which the sintered material 100 is heat-treated in warm water, such as boiling water. The warm water may be pure water or a phosphoric acid solution containing dissolved phosphoric acid. The concentration of the phosphoric acid solution may be, for example, 0.001 mL / L to 5 mL / L. A phosphoric acid solution of this concentration can be obtained, for example, by adjusting an 85% by weight phosphoric acid solution with pure water to the desired concentration. The addition of phosphoric acid enhances the surface cleaning effect. When the sintered body 110 is hydrated, a hydrated film of aluminum hydroxide is formed on the surface of the aluminum, and as described above, the hydrated film may be included in the outer shell 14, but the outer shell 14 may also include a hydrated film.

[0094] 4, the anodizing process is a process of anodizing the sintered material 100 to form an outer shell 14 including an anodized film on the surface of the aluminum metal particles 113. In the anodizing process, a coating layer 17, 117 including an anodized film containing aluminum oxide may be formed on the surface of the aluminum base material 105 on which the aluminum metal particles 113 are stacked. In the anodizing process, for example, an anode on which the sintered material 100 is placed and a cathode on which stainless steel (SUS) is placed are immersed in an electrolytic solution to perform electrolysis.

[0095] The electrolyte used in the anodization step may contain at least one selected from the group consisting of citric acid, boric acid, phosphoric acid, sulfuric acid, oxalic acid, and salts thereof. A barrier-type anodic oxide film can be formed by anodizing with an electrolyte containing citric acid, boric acid, phosphoric acid, or a salt thereof. When forming a barrier-type anodic oxide film, it is easier to form an anodic oxide film with a consistent thickness by controlling the voltage, compared to a porous-type anodic oxide film. A porous-type anodic oxide film can be formed by anodizing with an electrolyte containing sulfuric acid, oxalic acid, phosphoric acid, or a salt thereof. When forming a porous-type anodic oxide film, it is possible to form the anodic oxide film at a lower voltage than a barrier-type anodic oxide film, thereby reducing electricity costs. Examples of salts include ammonium salts, sodium salts, potassium salts, and silicates. From the viewpoint of availability, it is preferable that the salt contains at least one of an ammonium salt and a sodium salt.

[0096] The conditions for anodization are not particularly limited. For example, the electrolysis temperature may be 0°C or higher, 10°C or higher, 20°C or higher, 30°C or higher, 40°C or higher, or 50°C or higher. The electrolysis temperature may be 70°C or lower, 60°C or lower, or 50°C or lower. The electrolysis voltage may be 0.1V or higher or 1V or higher, depending on the type of electrolyte. The electrolysis voltage may be 500V or lower or 400V or lower, depending on the type of electrolyte. The electrolysis time may be 0.1 minutes or longer or 1 minute or longer. The electrolysis time may be 60 minutes or shorter or 20 minutes or shorter. Anodization may be performed in a single step or may be performed in multiple separate steps.

[0097] <Dissolving Process> As shown in Figure 5, the dissolving process is a process of dissolving aluminum metal particles 113 surrounded by outer shells 14. The dissolving process can be carried out, for example, by immersing the sintered material 100, on which the outer shells 14 have been formed in the anodizing process, in a dissolving solution. In the dissolving process, the high-purity aluminum, pure aluminum, and aluminum alloy contained in the aluminum metal particles 113 can be dissolved inside the outer shells 14 and eluted to the outside of the outer shells 14. This creates cavities 15 inside the outer shells 14. As a result, the outer shells 14 remain as shell-like structures, forming hollow particles 13. In other words, hollow aluminum particles including the outer shells 14 and the cavities 15 surrounded by the outer shells 14 are formed. Note that in the sintered body 110, a continuous series of outer shells 14 is formed on the outer surfaces of adjacent aluminum metal particles 113. Therefore, the anodization process leaves the outer shells 14 derived from the plurality of aluminum metal particles 113 in a continuous form, and the dissolution process dissolves the inside of the aluminum metal particles 113, forming a plurality of connected, continuous cavities 15 inside the outer shells 14. As a result, a skeleton 11 is formed in which the plurality of hollow particles 13 are aggregated.

[0098] The dissolving solution used to dissolve the aluminum metal particles 113 in the dissolving step may contain at least one acid selected from the group consisting of phosphoric acid, sulfuric acid, nitric acid, and salts thereof, or at least one alkali selected from the group consisting of sodium hydroxide and potassium hydroxide. Such a dissolving solution has excellent solubility for the aluminum metal particles 113. The dissolving solution preferably contains, for example, phosphoric acid. The salt contained in the aqueous solution may contain at least one metal salt selected from the group consisting of aluminum, sodium, magnesium, calcium, and zinc.

[0099] When phosphoric acid is used as the dissolving solution, the concentration of phosphoric acid may be, for example, 0.1 g / L to 1000 g / L. The dissolving temperature in the dissolving step may be, for example, 50° C. to 80° C. The dissolving time in the dissolving step may be 1 minute to 60 minutes.

[0100] In the method for producing the analytical carrier 1, a metal member (aluminum member) 120 is formed by repeating an anodizing step and a dissolution step, in which a porous body 10 is laminated on an aluminum substrate 105. By repeating the anodizing step and the dissolution step, hollow particles 13 can be formed, each having an outer shell 14 including an anodized film containing aluminum oxide and a cavity 15 surrounded by the outer shell 14. While FIG. 5 illustrates the metal member 120 in which the porous body 10 is formed on one surface of the aluminum substrate 105, it is sufficient that the porous body 10 is formed on at least one surface of the aluminum substrate 105, and the porous body 10 may be formed on both surfaces of the aluminum substrate 105. That is, the metal member 120 may include the porous body 10 laminated on one surface of the aluminum substrate 105, or the porous body 10 may be formed on both surfaces of the aluminum substrate 105.

[0101] In the method for manufacturing the analytical support 1, the anodization step and the dissolution step are alternately repeated, and the number of times the anodization step and the dissolution step are repeated may be from 2 to 20 times. The number of times the anodization step and the dissolution step are repeated is not particularly limited, as it is affected by the voltage conditions of the anodization step and the processing time of the dissolution step. However, by repeating the anodization step or more, the amount of metallic aluminum remaining in the hollow particles 13 can be reduced, thereby improving the light transmittance of the porous body 10. This makes it possible to provide an analytical support 1 with high visibility. Furthermore, since the amount of residual metallic aluminum is unlikely to be further reduced even if the number of repetitions exceeds 20, work efficiency can be improved by limiting the number of repetitions to 20 or less. The number of repetitions may be, for example, 2 or more, 3 or more, 5 or more, or 8 or more. The number of times the anodization step and the dissolution step are repeated may be 15 or less, or 10 or less.

[0102] In the dissolving process, the high-purity aluminum, pure aluminum, and aluminum alloy contained in the aluminum base 105 can be dissolved inside the aluminum base 105 and eluted to the outside of the aluminum base 105. This creates a space inside the aluminum base 105 equivalent to the amount of eluted material. Here, dissolution in the dissolving process proceeds from the aluminum metal particles 113 to a location where the aluminum base 105 and the aluminum metal particles 113 are sintered together and where high-purity aluminum, pure aluminum, and aluminum alloys are present. For this reason, dissolution is more likely to proceed on the side where the coating layer 17, which is formed on the same side as the porous body 10 and is connected to the porous body 10 side via the communicating holes 18, is present than on the side where the coating layer 117, which is formed on the opposite side from the porous body 10 and is formed to cover the entire outer surface of the aluminum base 105, is present. As the dissolution of the porous body 10 proceeds in this manner, the aluminum substrate 105 on the same side as the porous body 10, which is present near the boundary between the aluminum substrate 105 and the coating layer 17, dissolves, leaving a portion that connects the aluminum substrate 105 on the opposite side of the porous body 10 to the coating layer 17. If the dissolution proceeds too rapidly, the aluminum substrate 105 and the coating layer 17 will peel off before the subsequent lamination step, reducing handleability and making it difficult to prepare an analytical carrier 1 in which the porous body 10 is supported on the support 20. If the dissolution proceeds too slowly, it will be difficult to separate the porous body 10 from the aluminum substrate 105 in the subsequent peeling step. For this reason, it is preferable to continue dissolution until the porous body 10 side of the aluminum substrate 105 and the coating layer 17 are partially connected.

[0103] <Lamination Process> As shown in Figure 6, the lamination process is a process in which the porous body 10 of the metal member 120 is adhered to the support 20 to form a laminate 130 in which the aluminum substrate 105, the porous body 10, and the support 20 are laminated in this order. In the lamination process, the adhesive layer 22 is adhered to the outer shell 14. By adhering the porous body 10 to the support 20, the porous body 10 can be supported by the support 20. Furthermore, by laminating the aluminum substrate 105, the porous body 10, and the support 20 in this order, the aluminum substrate 105 can be easily peeled off in the peeling process described below. Figure 6 illustrates an example of a laminate 130 in which the support 20 is adhered to the porous body 10 formed on one surface of the aluminum substrate 105. However, it is sufficient that the support 20 is adhered to at least one surface of the aluminum substrate 105, and the support 20 may be adhered to both surfaces of the aluminum substrate 105. That is, the metal member 120 may have a support 20 laminated on a porous body 10 formed on one side of the aluminum base material 105, or may have a support 20 laminated on a porous body 10 formed on both sides of the aluminum base material 105.

[0104] The support 20 before the porous body 10 is adhered is placed on a white reflection standard for which the measuring instrument is calibrated, and the L ＊ a ＊ b ＊ L in color system ＊ The value is 80 or more. ＊ By making the value 80 or more, it is possible to improve the distinguishability when water is absorbed. ＊ The value may be 85 or greater, 90 or greater, or 95 or greater.

[0105] As described above, the support 20 may include a support layer 21 and an adhesive layer 22. The porous body 10 and the support layer 21 may be bonded together with an adhesive. The adhesive may form the adhesive layer 22 by curing. The adhesive may be at least one selected from the group consisting of a solvent-volatile adhesive, a moisture-curing adhesive, a heat-curing adhesive, a curing agent-mixed adhesive, an anaerobic-curing adhesive, a UV-curing adhesive, a hot-melt adhesive, a pressure-sensitive adhesive, and a remoisturizing adhesive. The adhesive may include, for example, an ethylene-vinyl acetate copolymer, and may bond the porous body 10 and the support layer 21 together by thermal melting.

[0106] For example, the support 20 may be a single layer of thermoplastic resin, and the porous body 10 may be bonded to the support 20 by applying heat to one side of the support 20 to melt it. Alternatively, the support 20 may not be provided with a support layer 21, and an adhesive may be applied directly to the porous body 10 and cured to form the support 20 comprising the adhesive layer 22. Hereinafter, first to fourth lamination steps according to the configuration of the support 20 will be described.

[0107] As shown in FIG. 7 , the first lamination method can be applied to a support 20a in which a support layer 21a made of a thermoplastic resin and an adhesive layer 22a made of a thermoplastic resin having a softening temperature lower than the softening temperature of the support layer 21a are pre-laminated and integrated. Such a support 20a is commercially available as a laminate film. In the first lamination method, the support 20a is superimposed on the metal member 120 such that the adhesive layers 22a, 22a face both outer sides of the metal member 120, and the support layer 21a faces outward. In this superimposed state, heat is applied to the support 20a from both outer sides of the support layers 21a, 21a, to an extent that softens the adhesive layer 22a but does not soften the support layer 21a, thereby softening the adhesive layer 22a and bonding the adhesive layer 22a to the metal member 120. As a result, the metal member 120 and the adhesive layer 22a are bonded to each other on both sides of the metal member 120, and the support layer 21a is provided on the outside of the adhesive layer 22a, thereby forming a laminate 130a in which the support layer 21a, adhesive layer 22a, metal member 120, adhesive layer 22a, and support layer 21a are laminated in this order. When bonding is performed by applying heat to the adhesive layer 22a, it is preferable to sandwich the overlapped metal member 120 and supports 20a, 20a between at least a pair of rollers, and rotate the rollers to move the metal member 120 and supports 20a, 20a while applying heat and pressure to the metal member 120 and supports 20a, 20a.

[0108] As shown in Figure 8, the second lamination method can be applied when the support body 20b is formed by bonding an adhesive layer 22b made of a thermoplastic resin to a support layer 21b that is adhesive to the adhesive layer 22b. In the second lamination method, the adhesive layers 22b, 22b and the support layers 21b, 21b are laminated in this order on both outer sides of the metal member 120. In the laminated state, heat sufficient to soften the adhesive layer 22b is applied from both outer sides where the support layers 21b, 21b are located, thereby softening the adhesive layer 22b and bonding the adhesive layer 22b to the metal member 120 and the support layer 21b. As a result, the adhesive layer 22b and the support layer 21b are bonded and integrated to form the support body 20b, and the metal member 120 and the support layer 21b are bonded via the adhesive layer 22b. Furthermore, by bonding the metal member 120 and the adhesive layer 22b on both sides of the metal member 120 and providing the support layer 21b on the outside of the adhesive layer 22b, it is possible to form a laminate 130b in which the support layer 21b, adhesive layer 22b, metal member 120, adhesive layer 22b, and support layer 21b are laminated in this order. When bonding is performed by applying heat to the adhesive layer 22b, it is preferable to sandwich the laminated metal member 120, adhesive layers 22b, 22b, and support layers 21b, 21b, between at least a pair of rollers, and rotate the rollers to move the metal member 120, adhesive layers 22b, 22b, and support layers 21b, 21b, while applying heat and pressure to the metal member 120, adhesive layers 22b, 22b, and support layers 21b, 21b.

[0109] The third lamination method can be applied when the support 20c consists solely of an adhesive layer 22c made of a thermoplastic resin. In the third lamination method, adhesive layers 22c, 22c, and a release material 25 are laminated in this order on both outer sides of the metal member 120. The release material 25 is preferably a sheet-like material that does not soften when heated and can transfer heat to the adhesive layer 22c without adhering to the adhesive layer 22c, 22c. The release material 25 can be, for example, a paper sheet (release paper). With the support 20c and the release material 25 laminated together, heat sufficient to soften the adhesive layer 22 is applied from both outer sides where the release materials 25 are located, softening the adhesive layer 22 and bonding the adhesive layer 22 to the metal member 120 in the same manner as in the first and second lamination methods. After bonding, the release material 25 is peeled off from the adhesive layer 22, as shown in FIG. 9 . As a result, the metal member 120 and the adhesive layer 22 are bonded to each other on both sides of the metal member 120, thereby forming a laminate 130c in which the adhesive layer 22, the metal member 120, and the adhesive layer 22 are laminated in this order. When bonding is performed by applying heat to the adhesive layer 22, it is preferable to sandwich the laminated metal member 120, the adhesive layers 22, 22, and the peeling body 25 between at least a pair of rollers, and rotate the rollers to move the metal member 120, the adhesive layers 22, 22, and the peeling body 25 while applying heat and pressure to the metal member 120, the adhesive layers 22, 22, and the peeling body 25.

[0110] The fourth lamination method can be applied when the support body 20d is formed by bonding an adhesive layer 22d made of an adhesive (pressure-sensitive adhesive) to a support layer 21d that is adhesive to the adhesive layer 22d. As shown in FIG. 10 , in the fourth lamination method, an adhesive is first applied to one side of each of the support layers 21d to form the adhesive layers 22d. Next, the support layers 21d with the adhesive layers 22d formed thereon are superimposed on both outer sides of the metal member 120, with the adhesive layers 22d facing the metal member 120. In the superimposed state, a force sufficient to bond the adhesive layer 22d to the metal member 120 and the support layer 21d is applied from both outer sides of the support layers 21d, thereby bonding the adhesive layer 22d to the metal member 120 and the support layer 21d. As a result, the adhesive layer 22d and the support layer 21d are bonded together to form the support body 20d, and the metal member 120 and the support layer 21d are bonded together via the adhesive layer 22d. Furthermore, by bonding the metal member 120 to the adhesive layers 22d on both sides of the metal member 120 and providing the support layer 21d outside the adhesive layer 22b, it is possible to form a laminate 130d in which the support layer 21d, adhesive layer 22d, metal member 120, adhesive layer 22d, and support layer 21d are laminated in this order. When applying force to the adhesive layer 22b to perform adhesion, it is preferable to sandwich the superimposed metal member 120, adhesive layers 22d, 22d, and support layers 21d, 21d between at least a pair of rollers, and rotate the rollers while applying pressure to the metal member 120, adhesive layers 22d, 22d, and support layers 21d, 21d to move the metal member 120, adhesive layers 22d, 22d, and support layers 21d, 21d.

[0111] <Peeling Step> As shown in Fig. 11 , the peeling step is a step of dissolving the aluminum substrate 105 and peeling the aluminum substrate 105 from the laminate 130 to form the analytical carrier 1. Peeling the aluminum substrate 105 from the laminate 130 makes the porous body 10 visible. Fig. 11 illustrates the laminate 130 from which the aluminum substrate 105 and the coating layer 117 have been peeled off. However, it is sufficient that the analytical carrier 1 is formed from at least one surface of the laminate 130 by peeling off the aluminum substrate 105, and the analytical carrier 1 may also be formed from both surfaces of the laminate 130. That is, the laminate 130 subjected to the peeling step may have the support 20 laminated on the porous body 10 formed on one surface of the aluminum substrate 105, or may have the support 20 laminated on the porous body 10 formed on both surfaces of the aluminum substrate 105.

[0112] In the stripping step, the aluminum base 105 may be dissolved using a stripping solution. In the stripping step, 10% or more, 20% or more, 30% or more, 40% or more, 50% or more, 60% or more, 70% or more, 80% or more, or 90% or more of the aluminum base 105 may be dissolved.

[0113] In the peeling step, the aluminum substrate 105 may be dissolved with an acid solution or an alkaline solution to separate the coating layer 117 formed on the surface of the aluminum substrate 105 opposite the porous body 10 from the porous body 10. This makes it possible to produce an analytical carrier 1 including the porous body 10 including the coating layer 17 and the support 20. The aluminum substrate 105 may be dissolved, and the coating layer 117 may be naturally peeled off from the porous body 10. Alternatively, the aluminum substrate 105 may be partially dissolved, and the aluminum substrate 105 may be forcibly peeled off manually or mechanically from the laminate 130. Alternatively, the aluminum substrate 105 may be forcibly peeled off manually or mechanically from the laminate 130 after the lamination step without dissolving the aluminum substrate 105.

[0114] The stripping solution used in the stripping step may contain at least one alkali selected from the group consisting of sodium hydroxide and potassium hydroxide. Stripping solutions containing these alkalis have low viscosity and tend to penetrate deep into the porous body 10. Therefore, the use of such a stripping solution can promote the stripping of the aluminum substrate 105. The stripping solution may be an aqueous solution, such as an aqueous solution containing sodium hydroxide.

[0115] The stripping solution used in the stripping step may contain at least one organic acid selected from the group consisting of citric acid and gluconic acid. Stripping solutions containing these organic acids can convert dissolved aluminum into a complex in the stripping solution. Therefore, by using these stripping solutions, it is possible to suppress aluminum precipitation and improve the stability of the stripping solution. The stripping solution may contain the above-mentioned alkali and organic acid. The stripping solution may contain, for example, sodium hydroxide and citric acid.

[0116] The stripping solution used in the stripping step may contain at least one acid selected from the group consisting of phosphoric acid, sulfuric acid, and nitric acid. Stripping solutions containing these acids can also strip the aluminum substrate 105. The stripping solution may contain, for example, phosphoric acid, or may contain phosphoric acid and sulfuric acid.

[0117] The temperature of the stripping solution in the stripping step may be 30°C or higher and 70°C or lower. By setting the temperature of the stripping solution to 30°C or higher, the stripping of the aluminum substrate 105 can be promoted and the time required for the stripping step can be shortened. By setting the temperature of the stripping solution to 70°C or lower, the generation of excessive bubbles can be suppressed. This makes it possible to suppress damage to the porous body 10 in the stripping step.

[0118] In the stripping process, the aluminum substrate 105 may be stripped in a single step using only one type of stripper. Alternatively, the stripping process may involve stripping the aluminum substrate 105 in multiple steps. When the aluminum substrate 105 is stripped in multiple steps, the same type of stripper may be used in each step, or different types of stripper may be used. When the aluminum substrate 105 is stripped in multiple steps, the stripping conditions, such as the stripping temperature and stripping time, may be the same or different in each step. Alternatively, after treating the aluminum substrate 105 with the stripper, it may be subjected to treatments such as neutralization with a solution containing phosphoric acid, washing with water, and drying.

[0119] <Effects> As described above, the method for manufacturing an analytical carrier 1 according to this embodiment is a method for manufacturing an analytical carrier 1 including a porous body 10 and a support 20 that supports the porous body 10 on one side. The porous body 10 includes a skeleton 11 formed by an aggregation of a plurality of hollow particles 13 and a plurality of voids 12 surrounded by the skeleton 11. The hollow particles 13 have outer shells 14 including an anodized film containing aluminum oxide, and cavities 15 surrounded by the outer shells 14. The skeleton 11 is formed by the continuous outer shells 14 of the plurality of hollow particles 13. The manufacturing method includes a sintering step of sintering a plurality of aluminum metal particles 113 on an aluminum substrate 105 to obtain a sintered material 100 including the aluminum substrate 105 and a sintered body 110 formed by sintering the aluminum metal particles 113 on the aluminum substrate 105. The manufacturing method also includes an anodizing step of anodizing the sintered material 100 to form outer shells 14 including an anodized film on the surfaces of the aluminum metal particles 113. The manufacturing method includes a dissolving step of dissolving the aluminum metal particles 113 surrounded by the outer shells 14. In the manufacturing method, a metal member 120 is formed by repeatedly performing an anodizing step and a dissolving step, in which a porous body 10 is laminated on an aluminum substrate 105. The manufacturing method also includes a laminating step of adhering the porous body 10 of the metal member 120 to a support 20 to form a laminate 130 in which the aluminum substrate 105, the porous body 10, and the support 20 are laminated in this order. The manufacturing method also includes a peeling step of dissolving the aluminum substrate 105 and peeling it from the laminate 130. The aluminum metal particles 113 contain at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and an aluminum alloy. The aluminum substrate 105 contains at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and an aluminum alloy. The average particle diameter of the plurality of aluminum metal particles 113 is 0.1 μm or more and 20 μm or less. The packing ratio of the sintered body 110 is 10 vol% or more and 60 vol% or less. The support 20 before the porous body 10 is adhered is placed on a white reflection standard for which the measuring instrument is calibrated, and the L＊ a ＊ b ＊ L in color system ＊ The value is not less than 80. According to such a production method, the above-mentioned analytical carrier 1 can be produced.

[0120] Hereinafter, the present embodiment will be described in more detail with reference to examples and comparative examples, but the present embodiment is not limited to these.

[0121] Example 1 (Slurry Preparation) First, a slurry was prepared by uniformly dispersing 24 parts by mass of aluminum metal particles, 26 parts by mass of a pore-forming material, and 1 part by mass of a binder in 49 parts by mass of a solvent. The aluminum metal particles (AHZL58FN manufactured by Toyo Aluminum K.K.) were pure aluminum (JIS A1080) with a purity of 99.80% by mass or more. The aluminum metal particles were approximately spherical and had an average particle diameter of 3 μm. Starch (Nikka Co., Ltd., Nikka ...

[0122] (Sintering) The slurry was applied to one side of a 30 μm thick aluminum substrate (aluminum base material) using a Comma Coater (registered trademark) manufactured by Hirano Tecseed Co., Ltd., so that the thickness of the sintered body would be 50 μm. The slurry was dried at 100°C for 1.5 minutes, then heated at 350°C for 5 hours in an air atmosphere, and further sintered at 620°C to 640°C for 10 hours in an argon gas atmosphere. In this way, a sintered material was produced in which a sintered body was provided on an aluminum substrate.

[0123] (Hydration Treatment) Next, the sintered material was washed with a 5 g / L oxalic acid aqueous solution at 50° C. for 3 minutes. Thereafter, the sintered material was immersed in an aqueous solution prepared by adjusting the concentration of an 85% phosphoric acid aqueous solution with pure water to 0.5 mL / L at 85° C. for 3 minutes to perform hydration treatment. Next, the hydrated sintered material was washed with pure water at room temperature (30° C.) for 1 minute.

[0124] (First anodization) The hydrated sintered material was subjected to a first anodization to form an outer shell containing aluminum oxide on the surface of the aluminum metal particles. Specifically, the sintered material placed on the anode and stainless steel (SUS) placed on the cathode were immersed in an electrolyte at 50°C prepared by mixing pure water with 98% citric acid to a concentration of 0.1 g / L. Then, a current of 50 mA / cm was applied until the voltage reached 1000 V. ２ The sintered material was anodized by applying a current of 450 V and holding the voltage for 3 minutes. The sintered material that had undergone the first anodization was washed with pure water for 0.5 minutes.

[0125] (First dissolution treatment) The sintered material that had undergone the first anodization was immersed in an aqueous solution prepared by mixing 85% phosphoric acid with pure water at 500 mL / L (718 g / L) at 70° C. for 3.5 minutes to further dissolve the aluminum metal. The sintered material that had undergone the first dissolution treatment was then washed with pure water for 1 minute.

[0126] (Second Anodization) The sintered material that had undergone the first dissolution treatment was subjected to a second anodization to further form an outer shell containing aluminum oxide on the surface of the aluminum metal particles. Specifically, the sintered material placed on the anode and stainless steel (SUS) placed on the cathode were immersed in an electrolyte at 50°C prepared with pure water to contain 1 g / L of 98% citric acid and 0.1 g / L of 95% triammonium citrate. Then, a current of 50 mA / cm was applied until the voltage reached 50 V. ２ The sintered material was anodized by applying a current of 50 V to the sintered material and maintaining the voltage at 50 V for 3 minutes. The sintered material that had undergone the second anodization was washed with pure water for 0.5 minutes.

[0127] (Second dissolution treatment) The sintered material that had undergone the second anodization was subjected to a second dissolution treatment in which the aluminum metal was further dissolved by immersing it in an aqueous solution prepared by adjusting the concentration of 85% phosphoric acid aqueous solution with pure water to 500 mL / L (718 g / L) at 70° C. for 2.5 minutes. The sintered material that had undergone the second dissolution treatment was then washed with pure water for 1 minute.

[0128] (Third Anodization) The sintered material that had undergone the second dissolution treatment was subjected to a third anodization to further form an outer shell containing aluminum oxide on the surface of the aluminum metal particles. Specifically, the sintered material placed on the anode and stainless steel (SUS) placed on the cathode were immersed in an electrolyte at 50°C prepared with pure water so that the concentrations of 98% citric acid were 1 g / L and 95% triammonium citrate were 0.1 g / L. Then, a current of 50 mA / cm was applied until the voltage reached 50 V. ２ The anodizing treatment was carried out by applying a current of 50 V to the sintered material and holding the voltage at 50 V for 3 minutes. The sintered material that had been anodized the third time was washed with pure water for 0.5 minutes.

[0129] (Third dissolution treatment) The sintered material that had undergone the third anodization was subjected to a third dissolution treatment in which the aluminum metal was further dissolved by immersing the sintered material in an aqueous solution prepared by adjusting the concentration of an 85% aqueous phosphoric acid solution with pure water to 30 mL / L (43 g / L) at 70° C. for 30 minutes. The sintered material that had undergone the third dissolution treatment was then washed with pure water for 1 minute.

[0130] (Drying Treatment) The sintered material that had been subjected to the third melting treatment was dried at 200° C. for 2 minutes to produce the aluminum member according to this example.

[0131] (Lamination Treatment) The lamination treatment was carried out by lamination method 1. Lamination method 1 is as follows.

[0132] (Lamination Method 1) Using a transparent laminate film as a support, the aluminum member and the laminate film were laminated on both sides of the aluminum member prepared as described above, with the laminate film corresponding to the support being superimposed on both sides, to produce a laminate in which the laminate film was laminated on both sides of the aluminum member. A Fellowes laminate film (PET / LDPE / EVA, thickness 100 μm, GLOSS) was used as the laminate film. An EVA film was used as the adhesive layer, and a PET / LDPE film was used as the support layer. A Fellowes Proteus A3 laminator was used, and the aluminum member was laminated on the laminate film at a lamination speed of approximately 1.4 cm / s so that the lamination temperature was 90°C. The laminate in which the aluminum member was laminated on the laminate film was cut to a width of 25 mm. Lamination Method 1 corresponds to the first lamination method described above.

[0133] (Peeling Treatment) Peeling treatment was carried out under peeling conditions 2. Peeling conditions 2 are as follows.

[0134] (Removal Condition 2) The 25 mm wide laminate obtained as described above was immersed for 30 minutes at 40°C in an aqueous solution prepared with pure water to give 450 g / L of sodium hydroxide and 10 g / L of citric acid. This treatment resulted in the aluminum substrate being peeled and removed from the laminate. The laminate was then neutralized by immersion for 5 minutes at 70°C in an aqueous solution prepared with pure water to give 40 mL / L of 85% phosphoric acid.

[0135] (Drying Treatment) The laminate from which the aluminum substrate had been removed was washed with pure water for 1 minute and dried at 50° C. for 10 minutes to prepare an analytical carrier according to this example.

[0136] Examples 2 to 26 Analytical carriers were prepared in the same manner as in Example 1, except for the conditions shown in Tables 1 to 7. Lamination methods 2 to 4 listed in Table 7 are as follows. Peeling conditions 1 and 3 listed in Table 2 are as follows. The aluminum metal particles used were as follows.

[0137] (Lamination Method 2) Using the adhesive layer and support layer shown in Table 1, the adhesive layer and support layer were superimposed on both sides of the aluminum member prepared as described above, and the adhesive layer, aluminum member, and support layer were laminated to produce a laminate in which supports consisting of the adhesive layer and support layer were laminated on both sides of the aluminum member. Using the same laminator as in Example 1, lamination was performed at a lamination speed of approximately 0.7 cm / s so that the lamination temperature was 140°C. The laminate was cut to a width of 25 mm. Lamination Method 2 corresponds to the second lamination method described above.

[0138] (Lamination Method 3) Using the adhesive layer shown in Table 1, the adhesive layer and release paper were superimposed on both sides of the aluminum member prepared as described above, and the adhesive layer and the aluminum member were laminated. The release paper was then peeled off to produce a laminate in which supports made of adhesive layers were laminated on both sides of the aluminum member. Using the same laminator as in Example 1, lamination was performed at a lamination speed of approximately 0.7 cm / s so that the lamination temperature was 140°C. The laminate was cut to a width of 25 mm. Lamination Method 3 corresponds to the third lamination method described above.

[0139] (Lamination Method 4) Using the adhesive layer and support layer shown in Table 1, adhesive was applied to both sides of the support layer to form an adhesive layer. Then, the support layer with the adhesive layer formed thereon was placed on both outer sides of the aluminum member prepared as described above, with the adhesive layer facing the aluminum member. The adhesive layer was then bonded to the aluminum member and support layer, producing a laminate in which a support consisting of an adhesive layer and a support layer was laminated on both sides of the aluminum member. Aron Alpha EXTRA Impact Resistant (cyanoacrylate adhesive) manufactured by Toagosei Co., Ltd. was used as the adhesive. The laminate was cut to a width of 25 mm. Lamination Method 4 corresponds to the fourth lamination method described above.

[0140] (Removal Condition 1) A 25 mm wide laminate obtained as described above was immersed for 3 minutes at 70° C. in an aqueous solution prepared with pure water to give 300 mL / L of 98% sulfuric acid and 300 mL / L of 85% aqueous phosphoric acid. Thereafter, the 25 mm wide laminate was immersed for 5 minutes at 70° C. in an aqueous solution prepared with pure water to give 500 mL / L of 85% aqueous phosphoric acid. By these treatments, the aluminum substrate was removed from the laminate.

[0141] (Peeling Condition 3) The 25 mm wide laminate obtained as described above was pulled at a speed of 5 mm / s by holding the ends of the analytical carrier portion and the aluminum substrate by hand in directions opposite to each other normal to the laminate, thereby removing the aluminum substrate from the laminate.

[0142] (Aluminum metal particles) Average particle size 1.8 μm: AHU091 manufactured by Toyo Aluminum K.K. Average particle size 5 μm: AHZL58CN manufactured by Toyo Aluminum K.K. Average particle size 9 μm: AHZL560F manufactured by Toyo Aluminum K.K. Average particle size 15 μm: AHZL530C manufactured by Toyo Aluminum K.K.

[0143] Comparative Example 1 An analytical carrier was prepared in the same manner as in Example 1, except that the aluminum member that had been subjected to the drying process was not subjected to any subsequent processes after lamination (lamination process) under the conditions shown in Tables 1 to 7.

[0144] Comparative Examples 2 and 3 Analytical carriers were prepared in the same manner as in Example 1, except that the conditions in Tables 1 to 7 were used.

[0145] [Reference Example] Cytiva nitrocellulose membrane AE99 was used as the analytical carrier.

[0146] [Evaluation] The analytical carriers of the examples were evaluated as follows.

[0147] (Packaging ratio) The packing ratio of the sintered body was measured by the following procedure for the sintered material before hydration treatment: ２A measurement sample of 1000 mm was cut out. Measurements and calculations were then carried out on the measurement sample. (Note that in this specification, the projected area refers to the horizontal projected area of ​​the sintered material or sintered compact when viewed in a plane in the same direction as the thickness direction.) - The mass Ma (g) of the entire sintered material, which is the combination of the sintered compact and the aluminum base material, was measured. - The thickness Wa (cm) of the entire sintered compact was measured with a micrometer. - The thickness Wb (cm) of the aluminum base material portion was measured with a micrometer. - The mass Mb (g) of the aluminum base material portion was calculated by Mb = density of aluminum base material × Wb × 100. In this example, the density of the aluminum base material and the sintered compact portion made of aluminum material was set to 2.7 g / cm ３ The calculation was made as follows. - The mass Mc (g) of the sintered body portion was calculated as Mc = Ma - Mb. - The mass Md (g) of the sintered body portion assuming a packing rate of 100% was calculated as Md = density of material of sintered body portion x (Wa - Wb) x projected area of ​​sintered body portion = 2.7 x (Wa - Wb) x 100. - The packing rate of the sintered body contained in the measurement sample was calculated as packing rate (%) = (Mc / Md) x 100.

[0148] (Porosity of porous body) The porosity of the porous body was obtained by dividing the cumulative pore volume of the porous body by the volume of the porous body. The cumulative pore volume of the porous body was obtained by integrating the pore volumes of pores having a pore diameter of 0.1 μm or more and 100 μm or less, which were measured by mercury intrusion porosimetry.

[0149] (Cross-sectional observation) The analytical carrier was immersed in liquid nitrogen to freeze it, and then the frozen analytical carrier was bent and broken, and platinum was vapor-deposited on the fracture surface to a platinum film thickness of about 5 nm, thereby obtaining a sample for cross-sectional observation. A cross-section of this observation sample in a plane perpendicular to the adhesive interface between the porous body and the adhesive layer was observed using a scanning electron microscope ULTRA Plus manufactured by Carl Zeiss Co., Ltd. to obtain a cross-sectional observation photograph (cross-sectional SEM image). The cross-sectional observation photograph of Example 5 is shown in Figure 12. In Figure 12, reference numeral 10 denotes the porous body, reference numeral 20 denotes the support, and reference numeral 80 denotes fragments of the porous body 10 that broke and scattered on the support when broken.

[0150] (Shell Thickness) The shell thickness was measured from cross-sectional SEM images obtained by the cross-sectional observation method described above. The shell thickness was measured at 10 points on the hollow particles and the average value was calculated to obtain the shell thickness.

[0151] (Thickness of porous body) The thickness of the porous body was measured from a cross-sectional SEM image obtained by observing using the above-mentioned cross-sectional observation method. The thickness of the porous body was measured as the thickness at the position where the thickness in the thickness direction of the material constituting the porous body was greatest within a 50 μm wide range in a direction parallel to the adhesive interface between the porous body and the adhesive layer. The thickness of the porous body was measured at five points in five different ranges and the average value was calculated to obtain the thickness of the porous body.

[0152] (Thickness of adhesive layer) The thickness of the adhesive layer was measured from a cross-sectional SEM image obtained by observation using the cross-sectional observation method described above. The thickness of the adhesive layer was measured as the thickness at the position where the thickness in the thickness direction of the material constituting the adhesive layer was greatest within a 50 μm wide range in a direction parallel to the adhesive interface between the porous body and the adhesive layer. The thickness of the adhesive layer was obtained by measuring the thickness of the adhesive layer at five points in five different ranges and calculating the average value. In the table, the support thickness and support layer thickness (substrate thickness) refer to the support thickness for Examples 1 to 22, and refer to the support layer thickness (substrate thickness) for Examples 23 to 26 and Comparative Examples 1 to 3.

[0153] (Thickness of the adhesive joint) The thickness of the adhesive joint was measured from a cross-sectional SEM image obtained by observation using the above-mentioned cross-sectional observation method. Within a 50 μm wide range in the direction parallel to the adhesive interface between the porous body and the adhesive layer, a line passing through the part of the porous body closest to the adhesive layer (the bottom) and parallel to the adhesive interface direction between the porous body and the adhesive layer, and a line passing through the part of the adhesive layer closest to the porous body (the top) and parallel to the adhesive interface direction between the porous body 10 and the adhesive layer 22 were drawn, and the distance between these two lines was measured as the thickness of the adhesive joint 23. The thickness of the adhesive joint was measured at five points in five different ranges and the average value was calculated to obtain the thickness of the adhesive joint.

[0154] (Surface Observation) A sample for surface observation was obtained by vapor-depositing platinum on the surface of the analytical carrier so that the platinum film thickness was approximately 5 nm. The porous body side surface of this sample for observation, i.e., the surface of the coating layer, was observed using a scanning electron microscope ULTRA Plus manufactured by Carl Zeiss Co., Ltd. to obtain a surface observation photograph (surface SEM image). The surface observation photograph of Example 5 is shown in Figure 13.

[0155] (Cumulative pore surface area of ​​porous body) The cumulative pore surface area of ​​the porous body was obtained by converting the pore volume of pores having a pore diameter of 0.1 μm or more and 100 μm or less, measured by mercury intrusion porosimetry, into a surface area for each pore diameter and integrating the converted volume. A graph showing the relationship between pore diameter and differential pore surface area for Example 5 and the Reference Example is shown in Figure 14. A graph showing the relationship between pore diameter and cumulative pore surface area for Example 5 and the Reference Example is shown in Figure 15.

[0156] (Average pore diameter (4V / A) of porous body) The average pore diameter (4V / A) of the porous body was calculated by the following formula: Average pore diameter of porous body = 4 × (cumulative pore volume of porous body) / (cumulative pore surface area of ​​porous body) The cumulative pore volume of the porous body and the cumulative pore surface area of ​​the porous body were obtained as described above.

[0157] (Cumulative pore surface area ratio) The ratio of the cumulative pore surface area of ​​pores having a pore diameter of 1 μm or more and less than 1 μm to the cumulative pore surface area of ​​pores having a pore diameter of 1 μm or more and less than 10 μm was calculated. As shown in FIG. 15, the cumulative pore surface area of ​​pores having a pore diameter of 1 μm or more and less than 10 μm was obtained by integrating the pore surface area of ​​pores having a pore diameter of 1 μm or more and less than 10 μm. Similarly, the cumulative pore surface area of ​​pores having a pore diameter of 0.1 μm or more and less than 1 μm was obtained by integrating the pore surface area of ​​pores having a pore diameter of 0.1 μm or more and less than 1 μm. The cumulative pore surface area of ​​the porous body was determined by mercury intrusion porosimetry as described above.

[0158] (Ratio of metallic aluminum to components constituting the porous body containing elemental aluminum) The metallic aluminum content of the components constituting the porous body containing elemental aluminum was measured as follows, and the ratio of metallic aluminum to components constituting the porous body containing elemental aluminum was calculated. 1. The analytical carrier was scraped with an SUS spatula to scrape off the porous body portion. 2. 2.0 g was weighed out from the scraped-off sample. 3. The metallic aluminum content (mass%) of the weighed sample to components constituting the porous body containing elemental aluminum was measured in accordance with the metallic aluminum decomposition and separation ICP atomic emission spectrometry method of JIS G2404:2022. 4. The ratio of metallic aluminum to components constituting the porous body containing elemental aluminum was calculated from the metallic aluminum content (mass%) of the components constituting the porous body containing elemental aluminum using the following formula: Ratio of metallic aluminum to components constituting the porous body containing elemental aluminum = (content of metallic aluminum to components constituting the porous body containing elemental aluminum) / (100 - (content of metallic aluminum to components constituting the porous body containing elemental aluminum))

[0159] (Arithmetic mean roughness Sa) The arithmetic mean roughness Sa of the surface on the porous body side of the analytical carrier according to the examples and comparative examples was measured in accordance with ISO 25178. The arithmetic mean roughness Sa was measured using a three-dimensional white light interference microscope Contour GT-I manufactured by Bruker AXS Co., Ltd., under the conditions of a measurement range of 60 μm × 79 μm, an objective lens of 115x, and an internal lens of 1x. For the analytical carriers of Examples 1 to 26 and Comparative Examples 2 and 3, the surface on the porous body side supported by the support of the analytical carrier was observed. For Comparative Example 1, the surface on the porous body side supported by the aluminum substrate was observed. For Reference Example 1, the surface of the nitrocellulose membrane supported by the backing sheet was observed. For each sample, a 60 μm × 60 μm image was acquired, and the surface roughness Sa (μm) was calculated. The surface roughness Sa was obtained by calculating the average value of the images from a total of five fields of view.

[0160] (Flow Rate) The flow rate was measured as follows. First, the analytical carrier was immersed in pure water so that the plane direction of the analytical carrier was perpendicular to the liquid surface. Then, the time it took for the water to be drawn up to a height of 4 cm from the liquid surface by capillary action after immersion of the analytical carrier in pure water was measured and evaluated as the flow rate.

[0161] (L when dry ＊ L value) When the analytical carrier with the dried porous body is placed on a white reflection standard with which the measuring instrument is calibrated, and the surface of the porous body in the analytical carrier is measured with the measuring instrument. ＊ a ＊ b ＊ L in color system ＊ The value was measured. ＊ The values ​​were measured using a 45° circular illumination and vertical light receiving color difference meter (CR-331C color difference meter manufactured by Konica Minolta Japan, Inc.) conforming to JIS Z8722. The white reflection standard used was a white calibration plate manufactured by MINOLTA, CR-A46 (Y: 92.7, x: 0.3129, y: 0.3189).

[0162] (L when absorbing water ＊ L value) The analytical carrier with the porous body absorbing water is placed on a white reflection standard with which the measuring instrument is calibrated, and the L value is measured by the measuring instrument on the surface of the porous body of the analytical carrier. ＊ a ＊ b ＊ L in color system ＊ The value was measured. Specifically, an analytical carrier cut into 3 cm x 3 cm pieces was placed on the bottom of a Petri dish filled with pure water to a depth of 5 mm, with the thickness direction perpendicular to the water surface. When the water had been absorbed up to the top end of the porous body of the analytical carrier, the analytical carrier was removed from the Petri dish and placed on a white reflective standard with the porous body surface of the analytical carrier facing upward. With the porous body of the analytical carrier having absorbed pure water, the surface on which the porous body of the analytical carrier was placed was measured in the same manner as above using a color difference meter (CR-331c manufactured by Konica Minolta).

[0163] (L of the support ＊L value) The support before adhering the porous body is placed on a white reflection standard with which the measuring instrument is calibrated, and the surface of the support to which the porous body is adhered is measured with the measuring instrument. ＊ a ＊ b ＊ L in color system ＊ The support was measured in the same manner as above using a color difference meter (CR-331c manufactured by Konica Minolta).

[0164] (Bending Test) As shown in FIG. 16 , 1 mm diameter holes 210 were drilled 5 mm from both ends of an analytical carrier 1 cut to a size of 10 mm × 100 mm. A nylon thread 220 with a diameter of 0.165 mm was threaded through the hole 210, and 10 g E-2 class weights 230 were attached to both ends of the analytical carrier 1. The analytical carrier 1 was placed on a stainless steel rod 240 with a diameter of 2 mm and a length of 5 cm, so that the porous body was in contact with the stainless steel rod 240 at the longitudinal center of the analytical carrier 1. As shown in FIG. 17 , the stainless steel rod 240 was slowly lifted until the weights 230 at both ends of the analytical carrier 1 floated, and then held for 10 seconds, after which the stainless steel rod 240 was returned to its original position. The surface of the porous body 10 was then visually observed and evaluated. When no wrinkles remained on the surface of the porous body and no peeling of the porous body occurred, the evaluation was "excellent." When wrinkles remained but no peeling of the porous body occurred, the evaluation was "fair." When wrinkles remained and the porous body peeled, the evaluation was "poor." It was evaluated that wrinkles remained when wrinkles of a size on the order of millimeters or larger that could be visually confirmed were confirmed. Furthermore, when the porous body 10 and the support 20 were separated within the surface of the analytical carrier 1, and the minimum width of the separated portion was 2 mm or more and the maximum length was 5 mm or more, it was evaluated that peeling of the porous body 10 occurred.

[0165] (Gold Colloid Test) SARS-CoV-2 nucleoprotein recombinant was diluted with pseudoserum to concentrations of 0 ng / mL to 100 ng / mL, and 100 μL of each solution was dropped onto the sample pad 340 of the test strip (see FIG. 18). The analytical carrier was left to stand for 20 minutes, and the absorbance of the test line and control line was measured using an immunochromatography reader (C10066-10, Hamamatsu Photonics). The results are shown in Tables 9 and 10 and FIG. 19. The test strip was prepared as follows.

[0166] 18 , an antibody-immobilized membrane 310, an absorption pad 320, a conjugate pad 330, and a sample pad 340 were attached to a backing sheet in this order, and then cut to a width of 5 mm to prepare a test strip 300. The following reagents and materials were prepared to prepare the test strip 300.

[0167] (1) Preparation of reagents and materials Antibody C706: Rabbit Monoclonal anti-SARS-CoV-2 Nucleoprotein C706, manufactured by HyTest Antibody C524: Rabbit Monoclonal anti-SARS-CoV-2 Nucleoprotein C524, manufactured by HyTest Anti-rabbit antibody: Goat anti-Rabbit IgG h+1 Affinity Purified A120 201A, manufactured by HyTest 5mM-PB (pH 7.0): 3mM disodium hydrogen phosphate, 2mM sodium dihydrogen phosphate dodecahydrate・ 1x PBS: sodium chloride 8g / L, sodium dihydrogen phosphate dodecahydrate 2.9g / L, potassium chloride 0.2g / L, potassium dihydrogen phosphate 0.2g / L ・ Potassium dihydrogen phosphate: Fujifilm Wako Pure Chemical Industries, #169-04245 ・ Sodium dihydrogen phosphate dodecahydrate: Fujifilm Wako Pure Chemical Industries, #193-02845 ・ Sodium chloride: Fujifilm Wako Pure Chemical Industries, #191-01665 ・ Gold colloid solution: Gold colloid solution-SC, particle size 40nm, Tanaka Kikinzoku ・ PEG: Polyethylene Glycol 20000, Fujifilm Wako Pure Chemical Industries ・ BSA (Albumin, from Bovine Serum, Globulin Free-HG): Fujifilm Wako Pure Chemical Industries・Storage buffer: 1% BSA, 0.1% sodium azide, 0.05% PEG, 20 mM Tris-HCl (pH 8.2), 150 mM NaCl ・Sodium azide: Fujifilm Wako Pure Chemical Industries ・Pseudo serum (ingredients: 1x PBS, BSA 2%, Tween 20 0.05%) ・Test line antibody solution: Antibody C524 was prepared using 0.5 mL of Ultracel 30K, and after replacing with 5 mM-PB, the final solution volume was adjusted to 1 mg / mL with 5 mM-PB to a final volume of 100 μL. ・Control line antibody solution: Anti-rabbit antibody was prepared using 0.5 mL of Ultracel 30K, and after replacing with 5 mM-PB, the final solution volume was adjusted to 0.5 mg / mL with 5 mM-PB to a final volume of 100 μL. Blocking buffer: 50 mM boric acid was adjusted to pH 8.5 by adding 50 mM borax little by little, and then 2% casein was added.Washing buffer: 50 mM Tris was adjusted to pH 7.5 by adding 1% hydrochloric acid little by little, and then 0.5% sucrose and 0.05% sodium cholate were added. Absorbent pad: Cellulose Fiber Sample Pads 20 x 300 mm 100PK (Merck Millipore, CFSP203000). Backing sheet: Pre-cut backing sheet (Nippon Engineering, 34042 / 11GL-56338). Sample pad: Glass Fiber Diagnostic Pad.

[0168] (2) Preparation of antibody-immobilized membrane: The antibody solution for the test line was drawn up into a 25 μL syringe (HAMILTON, 702SNR) equipped with a polyethylene capillary tube at its tip. The tip of the capillary tube was lightly brought into contact with the membrane so that the angle between the membrane surface and the capillary tube was 45°, and the test line was swept at a sweep speed of 24 mm / min while discharging the antibody solution at a discharge rate of 2.5 μL / min. The test line was swept to a position 7 mm from the bottom end of the membrane (18 mm from the top end).

[0169] The antibody solution for the control line was drawn up into a 25 μL syringe (HAMILTON, 702SNR) with a polyethylene capillary tube attached to the tip, different from the above. The tip of the capillary tube was lightly brought into contact with the membrane so that the angle between the membrane surface and the capillary tube was 45°, and the antibody solution was discharged at a discharge rate of 2.5 μL / min while sweeping the control line at a sweep rate of 24 mm / min. The control line was swept to a position 12 mm from the bottom end of the membrane (13 mm from the top end).

[0170] The membrane with the test and control lines swabbed was dried in a thermostatic bath at 50°C for 1 minute. The membrane, dried as described above, was placed in a Petri dish filled with blocking buffer to a height of 2 mm, with the test line facing downward relative to the control line, and allowed to stand for 2 minutes. The membrane was then submerged in blocking buffer and allowed to stand for 3 minutes, after which the membrane was lifted out and excess blocking buffer was shaken off. The membrane was then submerged in washing buffer and allowed to stand for 10 minutes, after which the membrane was lifted out and excess washing buffer was absorbed into paper, and the membrane was dried at 50°C for 10 minutes.

[0171] (3) Preparation of Conjugate Pads Antibody C706 was prepared at 50 μg / mL using 0.5 mL of Ultracel 30K, substituted with 5 mM PB, and then adjusted to a final volume of 100 μL with ultrapure water. 900 μL of gold colloid solution was added to a 2.0 mL tube containing 100 μL of 50 mM potassium dihydrogen phosphate (pH 8.0) and stirred. To this solution, 100 μL of the 50 μg / mL antibody C706 solution prepared as described above was added with stirring and allowed to stand at room temperature for 10 minutes. 55 μL of 1% PEG was further added to this solution and gently stirred. 110 μL of 10% BSA (pH 9.0) was further added to this solution and gently stirred. The solution was then centrifuged at 8000 G for 15 minutes at 4°C. After centrifugation, the supernatant was removed, leaving approximately 100 μL, and the mixture was dispersed using an ultrasonic cleaner. 2 mL of colloidal gold storage buffer was added to this dispersion, which was then centrifuged at 8000 G and 4°C for 15 minutes. After centrifugation, the supernatant was removed, leaving approximately 100 μL, and the mixture was dispersed using an ultrasonic cleaner. A 1.5 μL aliquot of the dispersed sensitized colloidal gold solution was measured for O.D. 520. The O.D. 520 measurement was converted and adjusted to O.D. 520 = 6.0 with colloidal gold storage buffer to prepare an antibody C706-sensitized colloidal gold solution. 420 μL of the antibody C706-sensitized colloidal gold solution, 420 μL of ultrapure water, and 840 μL of coating buffer were mixed in a 2 mL tube. The entire mixture was evenly applied to a glass fiber diagnostic pad (10 mm x 300 mm) using a pipette, and then placed in a desiccator and dried under reduced pressure for at least one day.

[0172]

[0173]

[0174]

[0175]

[0176]

[0177]

[0178]

[0179]

[0180]

[0181]

[0182] [Study] As shown in Table 8, the analytical carriers according to Examples 1 to 26 were L ＊ The support has a value of 80 or more. ＊ On the other hand, in the analytical carrier according to Comparative Example 1, the porous body was laminated on the support, and the aluminum substrate was not peeled off from the porous body. ＊ The value is below 80. Therefore, the L ＊ Although the value was 80 or more, the L ＊ The analytical carriers according to Comparative Examples 2 and 3 had a value of L ＊ The support has a value of less than 80. ＊ Although the value was 80 or more, the L ＊ The value was below 80.

[0183] Furthermore, as shown in Table 8, the analytical carriers according to Examples 1 to 26 were provided with a support having an adhesive layer, and therefore the bending test results were favorable, and no peeling of the porous body occurred. On the other hand, the analytical carrier according to Comparative Example 1 was not provided with a support having an adhesive layer, and the porous body and the substrate were integrated, so the bending test results were unfavorable, and peeling of the porous body occurred. These results demonstrate that the analytical carriers according to Examples 1 to 26 can prevent the porous body from peeling from the support even when subjected to bending deformation. Furthermore, the analytical carriers according to Examples 1 to 20 and 24 to 26 did not experience peeling of the porous body during the bending test, and no wrinkles remained. These results demonstrate that the thickness of the outer shell was within the desired range, the thickness of the porous body was within the desired range, and the support layer was made of a material that is resistant to plastic deformation, thereby preventing wrinkles from remaining.

[0184] As shown in Tables 9 and 10, the analytical carriers according to Examples 1 to 26 and Comparative Examples 2 and 3 had stronger signals at the test line and control line compared to the Reference Example. Here, as shown in Table 3 and FIG. 15, the cumulative pore surface area ratios for the Examples were 0.1 or greater and 10 or less. Meanwhile, the cumulative pore surface area ratio for the analytical carrier according to the Reference Example was 0.098. These results demonstrate that the analytical carrier according to the Examples has more pores with pore diameters of 0.1 μm or greater and less than 1 μm compared to the analytical carrier according to the Reference Example. It is believed that the analytical carrier according to the Examples had stronger signals at the test line and control line due to the increased amount of antibody bound to the porous body and the increased amount of gold colloid adsorbed to the antibody.

[0185] 12 , a cross-sectional SEM image of the analytical carrier 1 of Example 5 confirmed a structure in which the porous body 10 was supported by a support 20 made of EVA / LDPE / PET. It was confirmed that an adhesive layer 22 made of EVA was adhered to the outer shell 14 of the porous body 10. It was confirmed that a coating layer 17 was provided on the surface of the porous body 10 opposite the support 20, and that the coating layer 17 separated the inside and outside of the porous body 10, where the skeleton 11 and voids 12 were present.

[0186] 13 , it was confirmed that a coating layer 17 was provided on the surface of the porous body 10. It was confirmed that the hollow particles 13 and outer shells 14 inside the porous body 10 were covered with the coating layer 17, and that the hollow particles 13 and outer shells 14 were not exposed on the surface side. It was confirmed that the coating layer 17 had communicating holes 18 that communicated from the outside to the inside of the porous body 10.

[0187] The entire contents of Japanese Patent Application No. 2024-058092 (filing date: March 29, 2024) are incorporated herein by reference.

[0188] Although the present embodiment has been described above using examples and comparative examples, the present embodiment is not limited to these examples and comparative examples, and various modifications are possible within the scope of the gist of the present embodiment.

[0189] REFERENCE SIGNS LIST 1 analytical carrier 10 porous body 11 skeleton 12 void 13 hollow particle 14 outer shell 15 cavity 17 coating layer 18 communicating hole 20 support 100 sintered material 105 aluminum substrate 110 sintered body 113 aluminum metal particle 117 coating layer 120 metal member 130 laminate

Claims

1. An analytical carrier comprising a porous body and a support supporting the porous body on one side, wherein the porous body comprises a skeleton formed by an assembly of a plurality of hollow particles and a plurality of voids surrounded by the skeleton, the hollow particles have an outer shell including an anodized film containing aluminum oxide and a cavity surrounded by the outer shell, the skeleton is formed by the outer shells of the plurality of hollow particles being continuous, the porosity of the porous body is 50% by volume or more and less than 100% by volume, the average pore diameter of the porous body is 0.1 μm or more and 20 μm or less, and the analytical carrier into which the porous body has absorbed water is placed on a white reflection standard with which a measuring instrument has been calibrated, and the L when the surface of the porous body on the analytical carrier is measured with the measuring instrument is ＊ a ＊ b ＊ L in color system ＊ A support for analysis having a value of 80 or more.

2. The analytical carrier in which the porous body has dried is placed on a white reflection standard with which a measuring instrument is calibrated, and the L when the surface of the porous body in the analytical carrier is measured with the measuring instrument ＊ a ＊ b ＊ L in color system ＊ The analytical carrier according to claim 1, wherein the value is 80 or more.

3. An analytical carrier as described in claim 1 or 2, wherein the support has the porous body on one side, a first side, and the second side, the side opposite the first side, is exposed.

4. An analytical carrier according to any one of claims 1 to 3, wherein the ratio of metallic aluminum to the component constituting the porous body containing aluminum element contained in the porous body is 0 or more and 0.1 or less in mass ratio.

5. An analytical carrier according to any one of claims 1 to 4, wherein the support includes an adhesive layer that adheres to the porous body and a support layer that supports the adhesive layer, the adhesive layer adheres to the outer shell, and the adhesive layer is interposed between the porous body and the support layer.

6. The analytical carrier according to claim 5, wherein the adhesive layer contains at least one material selected from the group consisting of resin, elastomer, starch, and protein.

7. The analytical carrier according to claim 5 or 6, wherein the support layer contains at least one of a resin and glass.

8. An analytical carrier according to any one of claims 1 to 7, wherein the porous body is provided on a surface opposite to the support, the porous body comprising a coating layer including an anodized film containing aluminum oxide, the coating layer separating the inside of the porous body where the skeleton and voids exist from the outside of the porous body, the coating layer having communicating pores connecting the inside and outside of the porous body, the coating layer being formed continuously with the outer shells of the hollow particles, and the cavities of the hollow particles communicating with the outside of the porous body through the communicating pores.

9. The analytical carrier according to claim 8, wherein the surface roughness Sa of the porous body is 0.01 μm or more and less than 1.8 μm.

10. The analytical carrier according to any one of claims 1 to 9, wherein the thickness of the outer shell is 40 nm or more and 1000 nm or less.

11. The analytical carrier according to any one of claims 1 to 10, wherein the thickness of the porous body is 20 μm or more and 1 mm or less.

12. The cumulative pore surface area of ​​the porous body having a pore diameter of 0.1 μm or more and 100 μm or less is 0.1 m 2 / cm 3 More than 20m 2 / cm 3 The analytical carrier according to any one of claims 1 to 11, wherein:

13. An analytical carrier according to any one of claims 1 to 12, wherein the ratio of the cumulative pore surface area of ​​pores having a pore diameter of 0.1 μm or more and less than 1 μm to the cumulative pore surface area of ​​pores having a pore diameter of 1 μm or more and 10 μm or less is 0.1 or more and 10 or less.

14. The analytical carrier according to any one of claims 1 to 13, wherein the time required for water to be drawn up to a height of 4 cm by capillary action is 400 seconds or less.

15. An analytical carrier according to any one of claims 1 to 14, wherein the analytical carrier is cut to a size of 10 mm x 100 mm, 10 g weights are attached to both ends of the analytical carrier, the analytical carrier is placed on a stainless steel rod at the longitudinal center of the analytical carrier so that the porous body is in contact with the stainless steel rod, the analytical carrier is lifted until the weights at both ends of the analytical carrier float and held there for 10 seconds, and then the stainless steel rod is returned to its original position, and the porous body does not peel off.

16. An immunochromatographic test strip comprising an analytical carrier according to any one of claims 1 to 15.

17. A method for producing an analytical carrier comprising a porous body and a support supporting the porous body on one side, wherein the porous body comprises a skeleton formed by an assembly of a plurality of hollow particles and a plurality of voids surrounded by the skeleton, the hollow particles each having an outer shell including an anodized film containing aluminum oxide and a cavity surrounded by the outer shell, and the skeleton is formed by the outer shells of the plurality of hollow particles being continuous, the production method comprising: a sintering step of sintering a plurality of aluminum metal particles on an aluminum base to obtain a sintered material comprising the aluminum base and a sintered body formed by sintering the aluminum metal particles on the aluminum base; an anodizing step of anodizing the sintered material to form the outer shells including the anodized film on the surfaces of the aluminum metal particles; and a dissolving step of dissolving the aluminum metal particles surrounded by the outer shells, wherein the production method forms a metal member in which the porous body is laminated on the aluminum base by repeating the anodizing step and the dissolving step, the porous body of the metal member is adhered to the support to form a laminate in which the aluminum base, the porous body, and the support are laminated in this order; and a peeling step of dissolving the aluminum base and peeling the aluminum base from the laminate, wherein the aluminum metal particles contain at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and aluminum alloys, the aluminum base contains at least one type of aluminum selected from the group consisting of high-purity aluminum, pure aluminum, and aluminum alloys, the average particle diameter of the plurality of aluminum metal particles is 0.1 μm or more and 20 μm or less, the packing rate of the sintered body is 10% by volume or more and 60% by volume or less, and the L when the surface of the support to which the porous body is adhered is measured with the measuring instrument in a state where the support before the porous body is adhered is placed on a white reflection standard with which the measuring instrument is calibrated ＊ a ＊ b ＊ L in color system ＊ The method for producing an analytical carrier, wherein the value is 80 or more.

18. A method for producing an analytical carrier as described in claim 17, wherein in the anodizing step, a coating layer including an anodized film containing aluminum oxide is formed on the surface of the aluminum base on which the aluminum metal particles are laminated, and in the peeling step, the aluminum base is dissolved with an acid solution or an alkaline solution to separate the coating layer formed on the surface of the aluminum base opposite to the porous body from the porous body.

19. A method for producing an analytical carrier as described in claim 17 or 18, wherein the electrolyte used in the anodization step contains at least one selected from the group consisting of citric acid, boric acid, phosphoric acid, sulfuric acid, oxalic acid, and salts thereof.

20. A method for producing an analytical support according to any one of claims 17 to 19, wherein the anodizing step and the dissolving step are repeated alternately, and the number of repetitions of the anodizing step and the dissolving step is from 2 to 20.

21. A method for producing an analytical support according to any one of claims 17 to 20, wherein the dissolving solution used to dissolve the aluminum metal particles in the dissolving step contains at least one acid selected from the group consisting of phosphoric acid, sulfuric acid, nitric acid, and salts thereof, or at least one alkali selected from the group consisting of sodium hydroxide and potassium hydroxide.

22. A method for producing an analytical carrier according to any one of claims 17 to 21, wherein the stripping solution used in the stripping step contains at least one alkali selected from the group consisting of sodium hydroxide and potassium hydroxide.

23. The method for producing an analytical carrier according to claim 22, wherein the stripping solution used in the stripping step contains at least one organic acid selected from the group consisting of citric acid and gluconic acid.

24. A method for producing an analytical carrier according to any one of claims 17 to 21, wherein the stripping solution used in the stripping step contains at least one acid selected from the group consisting of phosphoric acid, sulfuric acid, and nitric acid.

25. A method for producing an analytical carrier according to any one of claims 17 to 24, wherein the temperature of the stripping solution in the stripping step is 30°C or higher and 70°C or lower.

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