Functional composition comprising diasorea bulbifera extract and plant-derived exosomes

Abstract

The present disclosure relates to a functional composition comprising a Dioscorea bulbifera extract and plant-derived exosomes and, more specifically, to a functional composition comprising a Dioscorea bulbifera fruit extract and plant-derived exosomes, thereby being usable as a cosmetic raw material and a health functional food raw material having excellent moisturizing and cell regeneration effects.

Description

Functional composition comprising sky horse extract and plant-derived exosomes

[0001] The present disclosure relates to a functional composition comprising an extract of the fruit of the sky berry and plant-derived exosomes, and more particularly, to a functional composition that can be used as a cosmetic raw material and a health functional food raw material having an excellent moisturizing effect and an excellent cell regeneration effect by comprising an extract of the fruit of the sky berry and plant-derived exosomes.

[0002] Dry skin caused by environmental and genetic factors can weaken the skin barrier and lead to various inflammatory conditions. Therefore, it's crucial to moisturize to maintain optimal skin moisture levels. Maintaining optimal skin moisture levels is challenging due to varying weather and lifestyle conditions, as well as various biological factors. Therefore, a variety of moisturizing cosmetics are available.

[0003] To impart moisturizing properties to cosmetics, various chemically manufactured and animal-derived moisturizers have been used. However, chemically manufactured moisturizers lack environmental friendliness during their manufacturing process. Furthermore, the use of various chemical raw materials has resulted in cases of human and environmental toxicity to workers. Even when added to cosmetics to impart moisturizing properties, controversy persists over the safety of chemicals on the skin and human body. Furthermore, with the continued publication of reports of skin damage, especially to sensitive skin, consumers are increasingly turning away from them. Furthermore, animal-derived moisturizers are not well-suited to the modern world's growing concern for animal welfare, leading to their continued rejection by consumers.

[0004] Meanwhile, Diascorea bulbifera is a plant also called fruit yam and vine yam, and is a plant that contains a lot of useful substances such as mucin, saponin, choline, arginine, dietary fiber, potassium, magnesium, calcium, minerals, and diosgenin. Despite the excellent functions and potential of Diascorea bulbifera, its functionality has not been properly studied to date, and research on various functional products utilizing it is also lacking.

[0005] In addition, exosomes can be classified into animal-derived exosomes and plant-derived exosomes, among which plant-derived exosomes are nano-sized particles secreted from plant cells, play a certain role in intercellular movement and absorption, and contain unique effective ingredients of the plant, so that indirect adoption of the effective ingredients is possible depending on which plant the exosome is derived from, and through this, the useful functions of the plant can be enjoyed. Furthermore, plant-derived exosomes have the advantages of being easy to mass-produce and having high stability compared to animal-derived exosomes.

[0006] Under these circumstances, there is an urgent need for a functional composition containing sky ginseng and plant-derived exosomes as a cosmetic raw material that has excellent skin moisturizing effects and beneficial ingredients for the skin, such as anti-inflammatory effects and skin repair effects, without using chemically manufactured moisturizers or animal-derived moisturizers.

[0007] The problem to be solved by the present disclosure is to provide a functional composition comprising a sky-ma extract and plant-derived exosomes having excellent moisturizing and cell regeneration effects that can be used as cosmetic raw materials.

[0008] Another problem that the present disclosure seeks to solve is to provide a functional composition comprising a sky-ma extract and plant-derived exosomes having low skin irritation and excellent skin penetration enhancing effect.

[0009] Another problem that the present disclosure seeks to solve is to provide a functional composition that can be used as a raw material for a health functional food containing a sky horse extract and plant-derived exosomes.

[0010] The problems to be solved by the present disclosure are not limited to the problems mentioned above, and problems to be solved by the present disclosure that are not mentioned can be clearly understood by a person having ordinary knowledge in the technical field to which the present disclosure belongs (“ordinary skilled person”) from the description below.

[0011] According to one embodiment of the present disclosure, a functional composition for a cosmetic raw material comprising a sky horse extract and a plant-derived exosome, wherein the plant-derived exosome is an exosome derived from any one plant raw material selected from lotus leaves, lotus roots, peony flowers, okra, goldenrod, and centella asiatica, can be provided.

[0012] For example, a functional composition for cosmetic raw materials can be provided, characterized in that the sky horse extract is included in an amount of 5 to 98 parts by weight based on 100 parts by weight of the entire functional composition, and the plant-derived exosome is included in an amount of 0.01 to 2.0 parts by weight based on 100 parts by weight of the entire functional composition.

[0013] For example, the above-mentioned sky yam extract may be obtained by extracting dried sky yam or raw sky yam using one or more solvents selected from the group consisting of water, ethyl acetate, butyl acetate, ethyl alcohol, isopropyl alcohol, butyl alcohol, hexane, chloroform, ethylene glycol, propylene glycol, propanol, acetone, benzene, ethanol, methanol, and butanol.

[0014] For example, the above-mentioned sky horse extract may contain 5 to 70 parts by weight of mucin based on 100 parts by weight of the entire sky horse extract.

[0015] For example, the above-mentioned sky-ma extract may contain 0.03 to 0.12 parts by weight of saponin based on 100 parts by weight of the entire sky-ma extract.

[0016] For example, the above-mentioned sky horse extract may contain choline in an amount of 0.03 to 0.12 parts by weight based on 100 parts by weight of the entire sky horse extract.

[0017] For example, the above-mentioned sky horse extract may contain arginine in an amount of 0.01 to 0.50 parts by weight based on 100 parts by weight of the entire sky horse extract.

[0018] For example, the above-mentioned sky horse extract may contain diosgenin in an amount of 0.001 to 0.003 parts by weight based on 100 parts by weight of the entire sky horse extract.

[0019] For example, the functional composition may include carrot seed oil as an additional ingredient, and the carrot seed oil may be included in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the entire functional composition.

[0020] For example, the functional composition may include a deciduous tree extract as an additional ingredient, and the deciduous tree extract may be included in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the total functional composition.

[0021] For example, the functional composition may include a senna leaf extract as an additional ingredient, and the senna leaf extract may be included in an amount of 0.02 to 0.04 parts by weight based on 100 parts by weight of the entire functional composition.

[0022] According to another embodiment of the present disclosure, a health functional food composition comprising a sky horse extract and a plant-derived exosome can be provided, characterized in that the plant-derived exosome is an exosome derived from any one plant raw material selected from lotus leaves, lotus roots, peony flowers, okra, goldenrod, and centella asiatica.

[0023] For example, a composition for a health functional food can be provided, characterized in that the extract of the sky horsetail is included in an amount of 5 to 98 parts by weight based on 100 parts by weight of the entire functional composition, and the plant-derived exosome is included in an amount of 0.01 to 2.0 parts by weight based on 100 parts by weight of the entire functional composition.

[0024] For example, the above-mentioned sky yam extract may be obtained by extracting dried sky yam or raw sky yam using one or more solvents selected from the group consisting of water, ethyl acetate, butyl acetate, ethyl alcohol, isopropyl alcohol, butyl alcohol, hexane, chloroform, ethylene glycol, propylene glycol, propanol, acetone, benzene, ethanol, methanol, and butanol.

[0025] For example, the above-mentioned sky horse extract may contain 5 to 70 parts by weight of mucin based on 100 parts by weight of the entire sky horse extract.

[0026] For example, the above-mentioned sky-ma extract may contain 0.03 to 0.12 parts by weight of saponin based on 100 parts by weight of the entire sky-ma extract.

[0027] For example, the above-mentioned sky horse extract may contain choline in an amount of 0.03 to 0.12 parts by weight based on 100 parts by weight of the entire sky horse extract.

[0028] For example, the above-mentioned sky horse extract may contain arginine in an amount of 0.01 to 0.50 parts by weight based on 100 parts by weight of the entire sky horse extract.

[0029] For example, the above-mentioned sky horse extract may contain diosgenin in an amount of 0.001 to 0.003 parts by weight based on 100 parts by weight of the entire sky horse extract.

[0030] For example, the functional composition may include carrot seed oil as an additional ingredient, and the carrot seed oil may be included in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the entire functional composition.

[0031] For example, the functional composition may include a deciduous tree extract as an additional ingredient, and the deciduous tree extract may be included in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the total functional composition.

[0032] For example, the functional composition may include a senna leaf extract as an additional ingredient, and the senna leaf extract may be included in an amount of 0.02 to 0.04 parts by weight based on 100 parts by weight of the entire functional composition.

[0033] According to another embodiment of the present disclosure, the sky horse extract may be a sky horse extract fermented liquid obtained by inoculating the sky horse extract with Bacillus subtilis.

[0034] According to another embodiment of the present disclosure, a diet food comprising a sky horse extract or a fermented product using a sky horse extract can be provided.

[0035] According to another embodiment of the present disclosure, a composition for a health functional food comprising a sky horse extract or a fermented product using a sky horse extract can be provided.

[0036] According to the present disclosure, a functional composition including a sky-ma extract and plant-derived exosomes having excellent moisturizing and cell regeneration effects that can be used as cosmetic raw materials can be provided.

[0037] In addition, according to the present disclosure, a functional composition including a sky-ma extract and plant-derived exosomes having low skin irritation and an excellent skin penetration enhancing effect can be provided.

[0038] In addition, according to the present disclosure, a functional composition that can be used as a health functional food raw material including a sky horse extract and plant-derived exosomes can be provided.

[0039] The excellent and / or useful effects according to the present disclosure are not limited to the effects of the present disclosure described above, and it should be understood that those skilled in the art will also be able to clearly recognize excellent and / or useful effects of the present disclosure that are not explicitly disclosed in the present disclosure based on the disclosure of the present specification, and that these are intentionally disclosed by the present specification and are clearly included in the scope of the present disclosure.

[0040] Figure 1 is a diagram exemplarily showing the composition of a functional composition according to the present disclosure.

[0041]

[0042] The terms or words used in this specification and claims are not intended to be construed as limited to their commonly used dictionary meanings, and those skilled in the art will clearly understand that the terms or words are used in the sense intended to convey the meaning of the present disclosure within the scope of expressing the concepts that this specification and claims are intended to self-evidently convey.

[0043] In addition, it will be clearly understood by those skilled in the art that the configurations described in the aspects, embodiments, examples, etc. of the present disclosure described in this specification are merely preferred examples presented at the time to enable those skilled in the art to understand and reproduce the present disclosure, and are not intended to limit the present disclosure thereto.

[0044] Furthermore, the descriptions and specific embodiments of each configuration described in this specification can be readily applied to the descriptions and specific embodiments of each other configuration. That is, all possible combinations of the various configurations and specific embodiments disclosed in this specification fall within the scope disclosed in this specification, as will be readily apparent to those skilled in the art.

[0045] The term "and / or" as used herein is a term that includes each and every combination of two or more of the items mentioned. In addition, when a singular term is used herein, it is disclosed including the plural unless otherwise specified.

[0046] The terms "comprise" and "comprising" used in this specification are terms that allow the presence or addition of items other than the items mentioned, and the terms "consist," "consist," "consisting," and "consisting" used in this specification are terms that do not allow the presence or addition of items other than the items mentioned.

[0047] The term "to" as used herein refers to a range of values ​​in which the range of values ​​indicated using the term "to" includes the values ​​described before and after the term as lower limits and upper limits, respectively. When a plurality of upper and / or lower limits are disclosed for each of an arbitrary numerical range, the numerical range discloses an arbitrary numerical range in which any one of the plurality of lower limits and any one of the plurality of upper limits are each the lower limit and the upper limit, respectively.

[0048] The terms “about” and / or “approximately” as used herein mean a numerical range between an upper value and a lower value that is 10% greater than the value indicated using “about” and / or “approximately” above.

[0049] The terms or words used in this specification and claims should not be construed as limited to their conventional dictionary meanings, but rather should be construed as meanings and concepts consistent with the technical spirit of the present disclosure, based on the principle that the inventor can appropriately define the concept of the term to best describe his or her invention. Therefore, the configurations described in the embodiments described in this specification are merely preferred embodiments of the present disclosure and do not represent all of the technical spirit of the present disclosure. Therefore, it should be understood that various equivalents and modified examples may exist as substitutes for them at the time of this application.

[0050] Meanwhile, each description and embodiment disclosed in this specification may also be applied to each other description and embodiment. That is, all combinations of the various elements disclosed in this specification fall within the scope of this disclosure, and descriptions omitted in one embodiment may be interpreted in the same manner as described in other embodiments. Furthermore, the scope of this disclosure is not limited by the specific descriptions described below.

[0051] According to one embodiment of the present disclosure, a functional composition for a cosmetic raw material including a sky horse extract and a plant-derived exosome can be provided.

[0052] For example, the functional composition for a cosmetic raw material may be provided as a functional composition including a sky-ma extract and a plant-derived exosome, wherein the plant-derived exosome is an exosome derived from any one plant raw material selected from lotus leaves, lotus roots, peony flowers, okra, goldenrod, and centella asiatica.

[0053] Sky yam is a type of yam. It is thicker and longer than regular yam, and has the characteristic of growing in a round shape without peeling. It grows mainly in warm regions, especially tropical and subtropical regions. Since it is a yam that bears fruit rather than growing in the ground, it is easy to cultivate and can be grown organically without pesticides. In addition, sky yam has various nutrients and health benefits, and contains a large amount of various effective ingredients. In particular, sky yam as a cosmetic ingredient contains diosgenin, which can slow down the skin aging process and keep the skin moisturized. It also contains mucin, a sticky mucus component, which can soften the skin and prevent various skin diseases.

[0054] Plant-derived exosomes are small extracellular vesicles secreted by cells to transmit information between cells. They are responsible for transporting various biological substances such as proteins, lipids, and nucleic acids. They also possess the characteristics of stem cells, such as cell differentiation, regeneration, and immunity. Therefore, when used as a cosmetic ingredient, they can be useful for improving skin health and treating inflammatory diseases.

[0055] For example, a functional composition for cosmetic raw materials can be provided, characterized in that the extract of the sky horse is included in an amount of 5 to 98 parts by weight based on 100 parts by weight of the entire functional composition, and the plant-derived exosome is included in an amount of 0.01 to 2.0 parts by weight based on 100 parts by weight of the entire functional composition.

[0056] If the above-mentioned sky-ma extract is included in an amount of less than 5 parts by weight based on 100 parts by weight of the entire functional composition, there is a problem that the skin improvement effect due to the inclusion of the sky-ma extract is minimal, and if it is included in an amount of more than 98 parts by weight based on 100 parts by weight of the entire functional composition, there is a problem that the viscosity of the composition increases and the processability decreases. Therefore, it is preferable that the content of the sky-ma extract satisfies the above numerical range.

[0057] If the plant-derived exosomes are included in an amount of less than 0.01 parts by weight based on 100 parts by weight of the entire functional composition, there is a problem that the skin improvement effect due to the inclusion of the plant-derived exosomes is minimal, and if the plant-derived exosomes are included in an amount of more than 2.0 parts by weight based on 100 parts by weight of the entire functional composition, the entire composition may be deteriorated due to denaturation of the included plant-derived component. Therefore, it is preferable that the content of the plant-derived exosomes satisfies the above numerical range.

[0058] For example, the above-mentioned sky yam extract may be obtained by extracting dried sky yam or raw sky yam using one or more solvents selected from the group consisting of water, ethyl acetate, butyl acetate, ethyl alcohol, isopropyl alcohol, butyl alcohol, hexane, chloroform, ethylene glycol, propylene glycol, propanol, acetone, benzene, ethanol, methanol, and butanol.

[0059] For example, the above-mentioned sky yam extract may contain 5 to 70 parts by weight of mucin based on 100 parts by weight of the entire sky yam extract. The above-mentioned mucin is a sticky component present in sky yam and is a type of glycoprotein, which has an excellent skin moisturizing effect, helps digestive health, can improve symptoms of gastric ulcers or hyperacidity, and can be used as a tonic that increases stamina, as well as helping digestive health, protecting the gastric mucosa, and activating digestive enzymes to promote digestion.

[0060] For example, the above-mentioned sky yam extract may contain 0.03 to 0.12 parts by weight of saponin based on 100 parts by weight of the entire sky yam extract. The above-mentioned saponin is an amphipathic glycoside present in various plant species and is a representative compound that enhances the human body's immunity, and is also used as a cardiotonic or diuretic in oriental medicine.

[0061] For example, the above-mentioned sky yam extract may contain choline in an amount of 0.03 to 0.12 parts by weight based on 100 parts by weight of the entire sky yam extract. The choline is a member of the vitamin B complex, a complex lipid component such as lecithin or plasmalogen, and a component of acetylcholine, a substance involved in nerve excitation transmission, and is used for various fat metabolism diseases as a fatty liver prevention factor, and is known to have a positive effect on brain activity and development.

[0062] For example, the above-mentioned sky yam extract may contain 0.01 to 0.50 parts by weight of arginine based on 100 parts by weight of the entire sky yam extract. The above-mentioned arginine is an alpha-amino acid used in the biosynthesis of proteins and is also called arginine. It is known to enhance mental and physical functions and promote hormone secretion, and is known to have the effect of a tonic and to be useful for improving stamina.

[0063] For example, the above-mentioned sky yam extract may contain 0.001 to 0.003 parts by weight of diosgenin based on 100 parts by weight of the entire sky yam extract. The above-mentioned diosgenin alleviates menopausal symptoms, and generally, during menopause, hormones decrease, causing unprecedented symptoms to appear. Diosgenin is used as a synthetic raw material for progesterone, male hormones, cortisone, etc., and provides a positive effect on hormone replenishment, thereby activating the body and alleviating menopausal symptoms.

[0064] In addition, the dietary fiber contained in the sky horse improves digestive function and helps control blood sugar levels, and it is rich in various minerals such as calcium and magnesium, which are necessary for bone and heart health, and it also contains many essential amino acids, which can help with muscle and bone health.

[0065] In addition, the saponins and dietary fiber contained in the sky horsetail reduce the amount of LDL cholesterol, which is negative cholesterol in the human body, and increase the amount of HDL cholesterol, which is positive cholesterol, and prevent waste from accumulating in the blood vessels, thus purifying the blood. In addition, potassium helps to discharge sodium from the body, which helps to stably manage blood pressure, thus preventing cardiovascular disease.

[0066] For example, the functional composition may include carrot seed oil as an additional ingredient, and the carrot seed oil may be included in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the entire functional composition.

[0067] Carrot seed oil is an oily component extracted from carrot seeds. While possessing a distinctive, strong odor, it possesses excellent antioxidant and skin-hydrating properties. Recently, it has also been confirmed to exhibit various anticancer properties. The composition according to the present disclosure comprises a combination of a celery extract, plant-derived exosomes, and carrot seed oil, thereby suppressing the off-flavor of carrot seed oil while exhibiting excellent skin-hydrating and anti-inflammatory effects.

[0068] When the carrot seed oil is included in an amount of less than 0.5 parts by weight based on 100 parts by weight of the entire functional composition, there is a problem that the skin improvement effect due to the inclusion of carrot seed oil is minimal, and when it is included in an amount of more than 1.5 parts by weight based on 100 parts by weight of the entire functional composition, there is a problem that the off-flavor becomes stronger and the degree of skin irritation increases. Therefore, it is preferable that the content of the carrot seed oil satisfies the above numerical range.

[0069] For example, the functional composition may include a deciduous tree extract as an additional ingredient, and the deciduous tree extract may be included in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the entire functional composition.

[0070] The extract of the genus Amurense is a component extracted from the genus Amurense, a deciduous broadleaf shrub with fruits and flowers that have antipyretic and anti-inflammatory effects. It has antibacterial properties and can soothe mild skin inflammation and reduce redness. The composition according to the present disclosure can further enhance the anti-inflammatory action of the composition by including the extract of the genus Amurense, plant-derived exosomes, and the extract of the genus Amurense.

[0071] If the above-mentioned extract of the pine tree is included in an amount of less than 0.5 parts by weight based on 100 parts by weight of the entire functional composition, there is a problem that the skin improvement effect due to the inclusion of the pine tree extract is minimal, and if it is included in an amount of more than 1.5 parts by weight based on 100 parts by weight of the entire functional composition, there is a problem that the composition becomes sticky and leaves a residue. Therefore, it is preferable that the content of the pine tree extract satisfies the above numerical range.

[0072] For example, the functional composition may include a senna leaf extract as an additional ingredient, and the senna leaf extract may be included in an amount of 0.02 to 0.04 parts by weight based on 100 parts by weight of the entire functional composition.

[0073] Senna leaf, also known as senna leaf, is a raw material known to be useful for promoting digestion and relieving constipation, and is also an ingredient with various anti-inflammatory and anti-pyretic properties. The composition according to the present disclosure can exhibit excellent skin moisturizing and anti-inflammatory effects by including a senna leaf extract, a plant-derived exosome, and a senna leaf extract together.

[0074] There is a problem that the skin improvement effect due to the inclusion of the senna leaf extract is minimal when the senna leaf extract is included in an amount less than 0.02 parts by weight based on 100 parts by weight of the entire functional composition, and there is a problem that skin irritation increases when the senna leaf extract is included in an amount greater than 0.04 parts by weight based on 100 parts by weight of the entire functional composition, so it is preferable that the content of the senna leaf extract satisfies the above numerical range.

[0075] According to another embodiment of the present disclosure, a health functional food composition comprising a sky horse extract and a plant-derived exosome can be provided, characterized in that the plant-derived exosome is an exosome derived from any one plant raw material selected from lotus leaves, lotus roots, peony flowers, okra, goldenrod, and centella asiatica.

[0076] For example, a composition for a health functional food can be provided, characterized in that the extract of the sky horsetail is included in an amount of 5 to 98 parts by weight based on 100 parts by weight of the entire functional composition, and the plant-derived exosome is included in an amount of 0.01 to 2.0 parts by weight based on 100 parts by weight of the entire functional composition.

[0077] For example, the above-mentioned sky yam extract may be obtained by extracting dried sky yam or raw sky yam using one or more solvents selected from the group consisting of water, ethyl acetate, butyl acetate, ethyl alcohol, isopropyl alcohol, butyl alcohol, hexane, chloroform, ethylene glycol, propylene glycol, propanol, acetone, benzene, ethanol, methanol, and butanol.

[0078] For example, the above-mentioned sky horse extract may contain 5 to 70 parts by weight of mucin based on 100 parts by weight of the entire sky horse extract.

[0079] For example, the above-mentioned sky-ma extract may contain 0.03 to 0.12 parts by weight of saponin based on 100 parts by weight of the entire sky-ma extract.

[0080] For example, the above-mentioned sky horse extract may contain choline in an amount of 0.03 to 0.12 parts by weight based on 100 parts by weight of the entire sky horse extract.

[0081] For example, the above-mentioned sky horse extract may contain arginine in an amount of 0.01 to 0.50 parts by weight based on 100 parts by weight of the entire sky horse extract.

[0082] For example, the above-mentioned sky horse extract may contain diosgenin in an amount of 0.001 to 0.003 parts by weight based on 100 parts by weight of the entire sky horse extract.

[0083] For example, the functional composition may include carrot seed oil as an additional ingredient, and the carrot seed oil may be included in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the entire functional composition.

[0084] For example, the functional composition may include a deciduous tree extract as an additional ingredient, and the deciduous tree extract may be included in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the entire functional composition.

[0085] For example, the functional composition may include a senna leaf extract as an additional ingredient, and the senna leaf extract may be included in an amount of 0.02 to 0.04 parts by weight based on 100 parts by weight of the entire functional composition.

[0086] According to another embodiment of the present disclosure, the sky horse extract may be a sky horse extract fermented liquid obtained by inoculating the sky horse extract with Bacillus subtilis.

[0087] Hereinafter, the present disclosure will be described in more detail using examples. Process conditions and preparatory steps not specified in the examples below may be process conditions or preparatory steps that are self-evident in the technical field to which the present disclosure pertains. A person skilled in the art will be able to select these conditions and reproduce the problem-solving principles of the present disclosure without difficulty based on the present disclosure.

[0088] In addition, in the manufacturing method according to the present disclosure, unless otherwise specified, each step constituting the manufacturing method is performed at room temperature (25°C), and it should be understood that each step is performed by means and tools that can be derived without much difficulty by a person skilled in the art.

[0089] Manufacturing Preparation Example 1: Preparation of raw sky horsetail extract

[0090] Sky yam was grown and used directly. 300 g of raw sky yam was cut, and 300 g of each solvent listed in Table 1 was added. The mixture was stirred at room temperature for 8 hours in an extractor equipped with a cooling condenser. The extract was extracted at 45°C for 12 hours, then filtered through a Whatman No. 6 filter paper to obtain a filtrate. The filtrate was concentrated under reduced pressure at 60°C in a distillation apparatus equipped with a cooling condenser to obtain an extract.

[0091] The extraction yield of Skyma according to each solvent is shown in Table 1 below.

[0092] Solvent Sky-green extract (g) Example 1 Water 320 Example 2 Ethyl acetate 170 Example 3 Butyl acetate 250 Example 4 Ethyl alcohol 330 Example 5 Isopropyl alcohol 230 Example 6 Butyl alcohol 170 Example 7 Ethylene glycol 210 Example 8 Propylene glycol 260 Example 9 Propanol 310 Example 10 Acetone 250 Example 11 Benzene 310

[0093] Manufacturing Preparation Example 2: Preparation of Dried Sky Yam Extract Sky Yam was grown and used directly. 500 g of sky yams, either freeze-dried or naturally dried, were added to each 500 g of the solvents in Table 3, and stirred at room temperature for 8 hours in an extractor equipped with a cooling condenser. The extract was extracted at 45°C for 12 hours, then filtered through a Whatman No. 6 filter paper to obtain a filtrate. The filtrate was concentrated under reduced pressure at 60°C in a distillation apparatus equipped with a cooling condenser to obtain an extract.

[0094] The extraction yield of Skyma according to each solvent is shown in Table 2 below.

[0095] Solvent Sky extract (g) Example 12 Water 620 Example 13 Ethyl acetate 370 Example 14 Butyl acetate 350 Example 15 Ethyl alcohol 330 Example 16 Isopropyl alcohol 330 Example 17 Butyl alcohol 370 Example 18 Ethylene glycol 310 Example 19 Propylene glycol 360 Example 20 Propanol 310 Example 21 Acetone 350 Example 22 Benzene 310

[0096] Manufacturing Example 1: Manufacturing of compositions of Examples 23 to 28 with added plant-derived exosomes The sky-ma extract obtained from Example 1 was left at room temperature, the solid content was removed by sieving, and stabilized. Then, 1.5 parts by weight of the plant-derived exosomes shown in Table 3 below were added and mixed by stirring to prepare compositions of Examples 23 to 28.

[0097] Added plant-derived exosome types Example 23 Exosomes derived from lotus leaves Example 24 Exosomes derived from lotus roots Example 25 Exosomes derived from peony flowers Example 26 Exosomes derived from oaks Example 27 Exosomes derived from gold chrysanthemum Example 28 Exosomes derived from centella asiatica

[0098] Manufacturing Example 3: Manufacturing of compositions of Examples 29 to 33 with added additional ingredients The composition of Example 23 was left at room temperature to stabilize, and then the additional ingredients shown in Table 4 below were added in the amounts described, stirred, and mixed to prepare compositions of Examples 29 to 33.

[0099] Unit: Weight Carrot Seed Oil Senna Leaf Extract Senna Leaf Extract Example 290.7--Example 300.70.7-Example 310.70.70.03 Example 320.3--Example 331.2--

[0100] Manufacturing Example 4: Manufacturing of fermented extract of Sky Horse

[0101] 100 kg of raw sky yam was washed with clean water, 500 kg of purified water was added, and then a high-speed stirring rotary grinder was used to grind it for 5 minutes. It was filtered using a high-pressure press to obtain 56 kg of filtrate. 20 kg of the obtained extract was placed in a fermentation tank, and Bacillus subtillis was inoculated while maintaining the temperature at 30 to 37°C and stirring at 100 to 200 rpm, and fermented for 30 days to prepare a sky yam extract fermentation liquid as a composition of Example 34.

[0102] Experimental Example 1: Skin Irritation Test (Patch Test)

[0103] The skin irritation properties of the above-mentioned Sky-Ma extract were confirmed through patch testing. The solvent-extracted extracts according to Examples 1 to 11 were applied to chambers (stiff aluminum) measuring 8 mm in diameter and 0.5 mm in depth, and five chambers were attached to each rectangular tape in two rows. The tapes were attached to the backs of 30 adults, and the patches were removed after two days, and skin irritation was assessed according to the ICDRG criteria.

[0104] ICDRG criteria - ?: questionable reaction, +: weakly positive, ++: strongly positive, +++: very strongly positive, -: negative, IR: irritant

[0105] Example Skin Irritation Example Skin Irritation Example Skin Irritation Example Example 1-Example 12-Example 23-Example 2-Example 13-Example 24-Example 3-Example 14-Example 25-Example 4-Example 15-Example 26-Example 5-Example 16-Example 27-Example 6-Example 17-Example 28-Example 7-Example 18-Example 29-Example 8-Example 19-Example 30-Example 9-Example 20-Example 31-Example 10-Example 21-Example 32-Example 11-Example 22-Example 33+

[0106] Referring to Table 5 above, it was confirmed that all of the compositions of Examples 1 to 31 according to the present disclosure did not cause skin irritation, and the composition of Example 33 containing carrot seed oil in an amount exceeding the preferred numerical range according to the present disclosure was confirmed to cause skin irritation.

[0107] Experimental Example 2: Verification of the Skin Penetration Enhancement Effect

[0108] The skin penetration enhancing effect of the extract of Sky Horse was measured using a transdermal absorption device (Franz-type diffusion cell, Hason) on guinea pig skin. Compositions 1 to 8 were prepared according to the compositions shown in Table 6 below and used as the experimental group, while Compositions 9 to 10 were used as the control group.

[0109] Unit: WeightPharmacologically active agentSolventSkin penetration enhancerTotalExperimental groupComposition 1Fresh yam extract 1.0Ethanol 97.00Cetyl alcohol 2.0100Composition 2Fresh yam extract 1.0Ethanol 97.00Tween 80 2.0100Composition 3Fresh yam extract 1.0Water 97.00Span 80 2.0100Composition 4Fresh yam extract 1.0Water 97.00Lanolin 2.0100Composition 5Dried yam extract 1.0Ethanol 97.00Lanolin 2.0100Composition 6Dried yam extract 1.0Ethanol 97.00Lanolin 2.0100Composition 7Dried yam extract 1.0 Water 97.00 Lanolin 2.0 100 Composition 8 Dried Sky-Sky Extract 1.0 Water 97.0 Lanolin 2.0 100 Control Composition 9 Raw Sky-Sky Extract 1.0 Ethanol 99.00-100 Composition 10 Dried Sky-Sky Extract 1.0 Ethanol 99.00-100

[0110] Guinea pig abdominal skin samples were collected over an area of ​​1 cm2, placed in a 0.9 cm diameter permeation cell, and then fixed with a clamp. 0.5 ml of each of the experimental and control groups were applied to one side of the skin, and the samples were left incubated at 32°C for 24 hours. Samples permeating the applied skin were collected and analyzed by HPLC to determine the extent of skin absorption. The results are shown in Table 7 below.

[0111] Unit skin absorption degree Composition 1++Composition 2++Composition 3++Composition 4++Composition 5++Composition 6++Composition 7+++Composition 8+++Composition 9+++Composition 10+++

[0112] Skin absorption level evaluation: +: Average absorption effect, ++: Fast absorption effect, +++: Very fast absorption effect

[0113] Experimental Example 3: Verification of Skin Moisturizing Effect

[0114] The skin moisturizing effect of the compositions of Examples 1 to 33 was confirmed as follows using the method for measuring moisturizing effect according to skin type.

[0115] Subjects were selected from among adult men and women aged 20 years or older who met the prescribed selection criteria and did not fall under any exclusion criteria. Subjects were excluded from the study if they experienced adverse events such as itching or erythema at the test site during the test, if they experienced excessive UV exposure to the test site during the test, or if they consumed excessive alcohol or smoke, which hindered the evaluation of the results, or if they had personal circumstances that made follow-up observation difficult during the test.

[0116] Each selected subject applied 2 ml of the composition to a 5 cm x 5 cm area of ​​the upper arm, selected as an area with less sweating and less external influence, in the morning and evening.

[0117] The performance of the composition was evaluated by having the subjects rest their skin for 30 minutes in a place with no air movement, no direct sunlight, and constant temperature and humidity conditions (20-24°C, 40-60% RH), and then exposing the area to be measured. Afterwards, the change in skin moisture content was measured three times using a corneometer to evaluate the effect of the test substance on skin moisture content. In addition, for the evaluation of adverse events, the occurrence of adverse events (erythema, edema, scaling, itching, stinging, burning, tightness, prickling) or other abnormalities was evaluated through questionnaires and visual inspection at each subject visit in the individual case report form, and the severity was recorded as mild, moderate, or severe. Additionally, we checked whether any cases of test suspension or dropout occurred and recorded them in the case report form.

[0118] Descriptive statistical analysis was performed using statistical processing programs such as SPSS and SAS, and the statistical significance level was set at p < 0.05. The results are presented in Table 8 below.

[0119] Example Moisturizing effect Example Moisturizing effect Example Moisturizing effect Example 1++++ Example 12+++ Example 23+++ Example 2+++ Example 13+++ Example 24+++ Example 3+++ Example 14+++ Example 25+++ Example 4++++ Example 15+++ Example 26+++ Example 5+++ Example 16+++ Example 27+++ Example 6+++ Example 17+++ Example 28+++ Example 7++++ Example 18+++ Example 29++++ Example 8+++ Example 19+++ Example 30++++ Example 9+++ Example 20+++ Example 31++++ Example 10++++ Example 21+++ Example 32++++Example 11+++Example 22+++Example 33++++

[0120] Skin moisturizing effect level evaluation: +: poor, ++: average, +++: good, ++++: very good

[0121] Experimental Example 4: Verification of Skin Anti-Inflammatory Effect

[0122] The skin anti-inflammatory effects of the compositions of Examples 1 to 33 were measured using inflammation-related biomarkers using cell lines, and the results are shown in Table 9 below.

[0123] Example Anti-inflammatory effect Example Anti-inflammatory effect Example Anti-inflammatory effect Example 1++++ Example 12++ Example 23++ Example 2++ Example 13++ Example 24++ Example 3++ Example 14+ Example 25++ Example 4++++ Example 15++ Example 26++ Example 5+ Example 16++ Example 27++ Example 6++ Example 17+++ Example 28++ ​​Example 7++++ Example 18+++ Example 29++++ Example 8++ Example 19++ Example 30++++ Example 9++ Example 20++ Example 31++++ Example 10++++ Example 21++ Example 32++++ Example 11+ Example 22++Example 33++++

[0124] Evaluation of the degree of cell line anti-inflammatory effect: +: slight, ++: moderate, +++: excellent, ++++: very excellent

[0125] Experimental Example 5: Evaluation of Antioxidant Effect (Anti-aging)

[0126] As an evaluation method of experimental raw materials used to inhibit aging, such as preventing wrinkles on the skin, the scavenging ability of the free radical DPPH was measured by measuring the degree to which the composition of the example scavenged the radical DPPH (2,2-Di(4-tert-octylphenyl)-1-picrylhydrazyl radical) and decolorized it by measuring the absorbance at 565 nm, and the results are shown in Table 10 below.

[0127] Free radical scavenging activity (%) Standard material Green tea extract Experimental concentration (0.1%) Sky ginseng extract Experimental concentration (0.1%) Experimental group Example 1 1845 Example 2 1832 Example 3 1833 Example 4 1845 Example 5 1836 Example 6 1835 Example 7 1845 Example 8 1833 Example 9 1837 Example 10 1845 Example 11 1840 Example 12 1835 Example 13 1845 Example 14 1838 Example 15 1836 Example 16 1839 Example 17 1845 Example 18 1845 Example 19 1832 Example 20 1831 Example 21 1837 Example 221836 Embodiment 231828 Embodiment 241831 Embodiment 251835 Embodiment 261837 Embodiment 271830 Embodiment 281835 Embodiment 291858 Embodiment 301862 Embodiment 311855 Embodiment 321866 Embodiment 331863

[0128] Referring to Table 10, it was confirmed that the compositions of Examples 1 to 33 according to the present disclosure had a very excellent free radical scavenging effect as measured by the DPPH method compared to 0.1% of green tea extract, which is a standard substance.

[0129] Experimental Example 6: Analysis of the effective components of the extract of Sky Horse

[0130] The extracts of 1 kg of raw sky yam and 1 kg of dried sky yam, each dried in the shade for one week, were analyzed for effective ingredients and contents for each sample using analytical devices such as HPLC, and the results are shown in Tables 11 and 12 below.

[0131] Unit: gMucinSaponinCholineArginineDietary FiberDiosgeninExample 150~7001.20.750.3770~1620.03Example 24200.80.710.411180.01Example 33870.70.700.321250.02Example 46200.90.830.381600.03Example 54600.90.800.351500.03Example 64900.70.750.301480.01Example 73850.50.530.211090.02Example 83680.50.500.191020.01Example 92650.50.510.121070.01 Example 104300.30.300.10900.02 Example 113600.30.300.09730.01

[0132] Unit: gMucinSaponinCholineArginineDietary FiberDiosgeninExample 150~6201.10.750.281390.03Example 23200.20.710.09970.01Example 32800.30.700.05980.02Example 44200.70.830.231140.03Example 52600.70.800.211030.03Example 62900.50.750.23960.01Example 73850.40.530.20870.02Example 82680.30.500.10500.01Example 92500.20.510.08380.01 Example 102870.30.300.101370.02 Example 112500.10.300.05540.01

[0133] Although the preferred embodiments of the present disclosure have been described in detail above, the scope of the present disclosure is not limited thereto, and various modifications and improvements made by those skilled in the art using the basic concepts of the present disclosure defined in the following claims also fall within the scope of the present disclosure.

Claims

1. A functional composition for a cosmetic raw material comprising a lotus leaf extract and a plant-derived exosome, wherein the plant-derived exosome is an exosome derived from any one plant raw material selected from lotus leaves, lotus roots, peony flowers, okra, goldenrod, and centella asiatica.

2. A functional composition for cosmetic raw materials, characterized in that, in paragraph 1, the sky-ma extract is included in an amount of 5 to 98 parts by weight based on 100 parts by weight of the entire functional composition, and the plant-derived exosome is included in an amount of 0.01 to 2.0 parts by weight based on 100 parts by weight of the entire functional composition.

3. In the second paragraph, the functional composition for cosmetic raw materials, characterized in that the extract of the sky yam is extracted from dried sky yam or raw sky yam using at least one solvent selected from the group consisting of water, ethyl acetate, butyl acetate, ethyl alcohol, isopropyl alcohol, butyl alcohol, hexane, chloroform, ethylene glycol, propylene glycol, propanol, acetone, benzene, ethanol, methanol, and butanol.

4. A functional composition for cosmetic raw materials, characterized in that in the third paragraph, the extract of the sky horse contains 5 to 70 parts by weight of mucin, 0.03 to 0.12 parts by weight of saponin, 0.03 to 0.12 parts by weight of choline, 0.01 to 0.50 parts by weight of arginine, and 0.001 to 0.003 parts by weight of diosgenin, based on 100 parts by weight of the entire sky horse extract.

5. A functional composition for cosmetic raw materials, characterized in that in paragraph 4, the functional composition comprises carrot seed oil as an additional ingredient, and the carrot seed oil is contained in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the total functional composition.

6. A functional composition for cosmetic raw materials, characterized in that in paragraph 5, the functional composition comprises an extract of the bark of the bark tree as an additional ingredient, and the extract of the bark of the bark tree is contained in an amount of 0.5 to 1.0 parts by weight based on 100 parts by weight of the total functional composition.

7. A functional composition for cosmetic raw materials, characterized in that in paragraph 6, the functional composition comprises a senna leaf extract as an additional ingredient, and the senna leaf extract is contained in an amount of 0.02 to 0.04 parts by weight based on 100 parts by weight of the total functional composition.

8. A composition for a health functional food comprising a sky horse extract and a plant-derived exosome, wherein the plant-derived exosome is an exosome derived from any one plant raw material selected from lotus leaves, lotus roots, peony flowers, okra, goldenrod, and centella asiatica.

9. A composition for a health functional food, characterized in that, in paragraph 8, the extract of the sky horse is included in an amount of 5 to 98 parts by weight based on 100 parts by weight of the entire functional composition, and the plant-derived exosome is included in an amount of 0.01 to 2.0 parts by weight based on 100 parts by weight of the entire functional composition.

10. A composition for a health functional food, characterized in that in the 9th paragraph, the extract of the sky yam is extracted from dried sky yam or raw sky yam using at least one solvent selected from the group consisting of water, ethyl acetate, butyl acetate, ethyl alcohol, isopropyl alcohol, butyl alcohol, hexane, chloroform, ethylene glycol, propylene glycol, propanol, acetone, benzene, ethanol, methanol, and butanol.

11. Diet food containing extract of Sky Horse or fermented product using Sky Horse extract.

12. Diet food containing extract of Sky Horse or fermented product using Sky Horse extract.

Images