Ophiocordyceps sinensis and method for artificially culturing new strains of ophiocordyceps sinensis

Abstract

Disclosed in the present invention are Ophiocordyceps sinensis and a method for artificially culturing a new strain of Ophiocordyceps sinensis. Ophiocordyceps sinensis QHYS2022-3 has the deposit number GDMCC No. 65577. Ophiocordyceps sinensis XZNQ2206 has the deposit number GDMCC No. 65578. Compared with the prior art, the use of an Ophiocordyceps sinensis solution cultured in a liquid culture medium supplemented with N-acetylglucosamine for the infection of the larvae of Hepialus armoricanus via the body surface can simultaneously achieve a high rate of fungal carriage (100% fungal carriage) and a high larva-stiffening rate (higher than 50%), thereby obtaining artificially cultivated Ophiocordyceps sinensis having a consistent fruiting body morphology with that of wild Ophiocordyceps sinensis from Yushu or Nagqu.

Description

Methods for the cultivation of Cordyceps sinensis fungus and new artificially cultured Cordyceps sinensis strains Technical fields:

[0001] This invention belongs to the field of artificial cultivation of Cordyceps sinensis, specifically involving the screening of Cordyceps sinensis strains from Yushu and Nagqu, and the method of obtaining new Cordyceps sinensis strains by infecting the larvae of the host insect, the small golden pheasant, on their body surface. Background technology:

[0002] Cordyceps sinensis is a traditional and rare edible and medicinal resource in my country, produced in high-altitude, cold regions above 3,000 meters in altitude, such as Tibet, Qinghai, Yunnan, Sichuan, and Gansu. Cordyceps sinensis possesses numerous medicinal properties, including "tonifying the lungs, strengthening the kidneys, replenishing essence and qi, and treating various deficiencies and injuries." These properties stem from its antibacterial, antiviral, anti-inflammatory, antitumor, and immunomodulatory functions. In my country's traditional tonic market, Cordyceps sinensis has long been highly regarded and trusted, widely used in medicine, food, and modern biotechnology, and is a popular product in international markets such as Japan, South Korea, Southeast Asian countries, and the United States.

[0003] Cordyceps sinensis is a fungus that infects the host insect, the ghost moth larvae, causing them to become mummified. Under suitable conditions, it forms a complex structure consisting of an insect (muffled insect) and a fungal fruiting body, which is Cordyceps sinensis. Cordyceps sinensis belongs to the phylum Ascomycota, class Sordariomycetes, order Hypocreales, family Ophiocordycipitaceae, and genus Ophiocordyceps.

[0004] The depletion of wild Cordyceps sinensis resources, strong market demand, and national policy protection have led to a surge in its market price. Wild Cordyceps sinensis has been listed as a national second-class protected species. In order to protect the ecological environment of the Qinghai-Tibet Plateau and wild Cordyceps sinensis resources, and to enable this resource to better serve the "Healthy China" initiative, the only option is artificial cultivation under suitable conditions.

[0005] Artificial cultivation methods for Cordyceps sinensis in low-altitude areas have been established. This method relies on breakthroughs in three major technologies: first, the efficient rearing of the ghost moth, the host insect of Cordyceps sinensis; second, the cultivation of Cordyceps sinensis fungi that efficiently infect the host; and third, the infection of ghost moth larvae with suitable Cordyceps sinensis fungi, which then forms Cordyceps sinensis under appropriate conditions.

[0006] Generally speaking, entomopathogenic fungi can enter the insect body through the body wall, natural openings, or digestive tract. In the artificial cultivation of Cordyceps sinensis, current methods for artificially infecting host larvae include: collecting ascospores of Cordyceps sinensis to infect ghost moth larvae; applying cultured Cordyceps sinensis spore liquid to the host surface, feeding the spores mixed with feed so they enter the host through the esophagus; and injecting budding spores into the ghost moth larvae. Currently, the most widely used method is to infect ghost moth larvae with collected Cordyceps sinensis ascospores, using strains from low-altitude areas in Gansu (mainly because Gansu Cordyceps sinensis strains are inexpensive). N-acetylglucosamine (fN-acetylglucosamine) and insect molting hormone (20-hydroxyecdysone) promote the transformation of Cordyceps sinensis budding spores into mycelia in vitro, while mannitol promotes the transformation of Cordyceps sinensis budding spores into mycelia in vivo. However, under current technology, the liquid culture of Cordyceps sinensis cannot effectively infect the larvae of the ghost moth on their body surface, let alone achieve a high bacterial carrying rate (100% bacterial carrying) and a high rate of worm mummification (above 50%), so as to obtain artificially cultivated Cordyceps sinensis with the same fruiting body morphology as wild Cordyceps sinensis from Yushu or Nagqu. Summary of the Invention:

[0007] The technical problem solved by this invention is to overcome the defects of the existing technology and provide a method for artificially cultivating a new strain of Cordyceps sinensis. The new strain is obtained by culturing Cordyceps sinensis strains, especially those from Yushu, Qinghai or Nagqu, Tibet, in a special culture medium, and then infecting the body surface of the strains with larvae of the small golden worm moth. The larvae of the small golden worm moth achieve a high bacterial carrying rate (100%) and a high rate of worm mummification (above 50%). The artificially cultivated Cordyceps sinensis obtained is consistent with the fruiting body morphology of wild Cordyceps sinensis from Yushu or Nagqu.

[0008] The first objective of this invention is to provide the application of N-acetylglucosamine in promoting infection of Hepialus larvae with Cordyceps sinensis, inducing Cordyceps sinensis to grow fruiting bodies in Hepialus larvae, and increasing the larval silicosis rate of Hepialus larvae.

[0009] Preferably, the N-acetylglucosamine is prepared by culturing Cordyceps sinensis in a culture medium and then using the bacterial solution to infect ghost moth larvae.

[0010] Preferably, the concentration of the N-acetylglucosamine is 0.01 to 1% by mass, more preferably 0.01 to 0.5% by mass.

[0011] Preferably, the Cordyceps sinensis fungus is Ophiocordyceps sinensis QHYS2022-3 or Ophiocordyceps sinensis XZNQ2206, wherein Ophiocordyceps sinensis QHYS2022-3 has the accession number GDMCC No. 65577; and Ophiocordyceps sinensis XZNQ2206 has the accession number GDMCC No. 65578.

[0012] Preferably, the bat moth larvae are small golden bat moth larvae, especially 1st to 5th instar larvae.

[0013] The second objective of this invention is to provide a method for artificially cultivating a new strain of Cordyceps sinensis, comprising the following steps:

[0014] 1) Add 0.01-1% N-acetylglucosamine to the liquid culture medium of Cordyceps sinensis, and then inoculate Cordyceps sinensis for culture to obtain a mixture of budding spores and mycelia.

[0015] 2) Infect the larvae of the ghost moth with a mixed liquid, then culture them to obtain Cordyceps sinensis.

[0016] Preferably, the Cordyceps sinensis fungus is Ophiocordyceps sinensis QHYS2022-3 or Ophiocordyceps sinensis XZNQ2206, wherein Ophiocordyceps sinensis QHYS2022-3 has the accession number GDMCC No. 65577; and Ophiocordyceps sinensis XZNQ2206 has the accession number GDMCC No. 65578.

[0017] Preferably, the bat moth larvae are small golden bat moth larvae, especially 1st to 5th instar larvae.

[0018] Preferably, the mixture is mixed with sterile coconut coir, and then the larvae of the ghost moth are infected on their body surface.

[0019] This invention also provides *Ophiocordyceps sinensis* QHYS2022-3, with accession number GDMCCNo.65577.

[0020] This invention also provides *Ophiocordyceps sinensis* XZNQ2206, with accession number GDMCCNo.65578.

[0021] Compared with existing technologies, using liquid culture medium supplemented with N-acetylglucosamine to infect the larvae of the ghost moth with Cordyceps sinensis can simultaneously achieve a high bacterial load (100% bacterial load) and a high larval silencing rate (above 50%), resulting in artificially cultivated Cordyceps sinensis with fruiting body morphology consistent with wild Cordyceps sinensis from Yushu or Nagqu.

[0022] [Corrected according to Rule 91 07.02.2025] Ophiocordyceps sinensis QHYS2022-3 was deposited on December 3, 2024 at the Guangdong Provincial Microbial Culture Collection Center (GDMCC), address: No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, postcode: 510070, accession number: GDMCC No. 65577.

[0023] [Correction based on Rule 91, 07.02.2025] Ophiocordyceps sinensis XZNQ2206 was deposited on December 3, 2024 at the Guangdong Provincial Microbial Culture Collection Center (GDMCC), address: No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, postcode: 510070, accession number: GDMCC No. 65578. Detailed implementation method:

[0024] [Correction based on Rule 91, 07.02.2025] The Cordyceps sinensis strain Ophiocordyceps sinensis QHYS2022-3 of the present invention was isolated from Yushu, Qinghai Province, and was deposited on December 3, 2024, at the Guangdong Provincial Microbial Culture Collection Center (GDMCC), address: No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, postcode: 510070, accession number: GDMCC No. 65577.

[0025] [Correction based on Rule 91, 07.02.2025] The Cordyceps sinensis strain Ophiocordyceps sinensis XZNQ2206 of this invention was isolated from Nagqu, Tibet, and was deposited on December 3, 2024, at the Guangdong Provincial Microbial Culture Collection Center (GDMCC), address: No. 100, Xianlie Middle Road, Yuexiu District, Guangzhou, Guangdong Province, postcode: 510070, accession number: GDMCC No. 65578.

[0026] The Cordyceps sinensis strain Ophiocordyceps sinensis GS-MQ, isolated from Gansu Province, is a commonly used infecting strain.

[0027] The following embodiments are further illustrations of the present invention, but not limitations thereof.

[0028] Example 1:

[0029] The Cordyceps sinensis culture medium used in this embodiment contains 0.01% N-acetylglucosamine.

[0030] 1. Preparation of Cordyceps sinensis bud spores

[0031] The culture medium for culturing budding spores was PPDA medium (by mass fraction: 2% glucose, 20% potato juice, 1% peptone, 0.3% KH₂PO₄, 0.15% MgSO₄·7H₂O, and 0.002% VB₁, with the remainder being H₂O (natural pH)), with 0.01% N-acetylglucosamine added. The preparation method involved thoroughly mixing all components and sterilizing before use. After accurate molecular identification, the conidia of *Cordyceps sinensis* were inoculated into the above PPDA medium and cultured on a shaker (120 rpm, 12℃) for 60 days. The cultured *Cordyceps sinensis* broth (containing approximately 10 budding spores and mycelia per milliliter) was then cultured. 6 (Each budding spore) is used to infect the body surface of first-instar larvae of the small golden pheasant.

[0032] Meanwhile, *Cordyceps sinensis* was cultured for 60 days in PPDA medium (2% glucose, 20% potato juice, 1% peptone, 0.3% KH2PO4, 0.15% MgSO4·7H2O, and 0.002% VB1, with the remainder being H2O (natural pH)) without the addition of 0.01% acetylglucosamine in a shaker (120 rpm, 12℃). The cultured *Cordyceps sinensis* broth (containing approximately 10 spores and hyphae per milliliter) was then cultured. 6 (Each budding spore) is used to infect the body surface of first-instar larvae of the small golden pheasant.

[0033] 2. Rearing of small golden bat moth larvae

[0034] By feeding the larvae of the ghost moth with a mixture of carrots (cut into 1-3cm pieces), Polygonum bulbiferum (cut into 1-3cm pieces), and Potentilla chinensis (uncut) in a mass ratio of 1:1:1, healthy larvae of the ghost moth, which can be used to infect the first instar larvae of the ghost moth, were obtained.

[0035] 3. Surface infection

[0036] The Cordyceps sinensis mycelium solutions obtained from the two culture media (approximately 10 μL per milliliter) 6 (1 budding spore) diluted with sterile water to approximately 10 ppm. 5 10 budding spores 5Add budding spores to 100g of sterile coconut coir (moisture content approximately 65%), then mix the budding spore-containing coconut coir with 100g of ghost moth larvae feed (Polygonum bulbiferum). Place approximately 500 eggs on top of the feed-mixed coconut coir. After hatching, the larvae are infected by the Cordyceps sinensis fungus cultured using the above method. Infected ghost moth larvae are then reared at 11-16℃ (feeding them a mixture of carrots, Polygonum bulbiferum, and Potentilla chinensis in a 1:1:1 mass ratio).

[0037] 4. Detection of bacteria carried by ghost moth larvae infected on their body surface

[0038] Three months after infection on the body surface, about 2-5 microliters of hemolymph were collected from the abdomen of the larvae using a microneedle (0.06 mm in diameter). After staining with Calcofluor White (Sigma), the morphology of the fungus was observed under a microscope, and a large number of budding spores could be observed. Three months after 3 months of external infection of *Cordyceps sinensis* larvae cultured on PPDA medium containing 0.01% acetylglucosamine, the bacterial carrier rate of *Cordyceps sinensis* strain Ophiocordyceps sinensis QHYS2022-3 from Yushu or Ophiocordyceps sinensis strain Ophiocordyceps sinensis XZNQ2206 from Nagqu reached 100%. Under the same conditions, the bacterial carrier rate of *Cordyceps sinensis* strain Ophiocordyceps sinensis GS-MQ from Gansu was approximately 29% after 3 months of external infection of *Cordyceps sinensis* larvae. The bacterial carrier rate of *Cordyceps sinensis* strain Ophiocordyceps sinensis GS-MQ from Gansu cultured on PPDA medium without acetylglucosamine was zero after 3 months of external infection of *Cordyceps sinensis* larvae.

[0039] 5. Sub-entity induction

[0040] Ghost moth larvae infected with Cordyceps sinensis strains from Yushu or Nagqu, cultured in PPDA medium containing 0.01% acetylglucosamine, underwent a growth period of 5-7 months, transforming from live larvae into mummified larvae. The heads of the mummified larvae split open, and fruiting bodies emerged from the split opening. The mummification rate was 51.5% for larvae infected with the Yushu strain, 49.5% for those infected with the Nagqu strain, and 8.5% for those infected with the Gansu strain. Artificially cultured Cordyceps sinensis, exhibiting morphology identical to wild Cordyceps sinensis, showed a fruiting body occurrence rate of 90.5% for the Yushu strain, 92.6% for the Nagqu strain, and 8.5% for the Gansu strain. Ghost moth larvae infected with Cordyceps sinensis strains from Yushu, Nagqu, or Gansu, cultured in PPDA medium without acetylglucosamine, had a mummification rate of zero because they did not carry the Cordyceps sinensis fungus.

[0041] Example 2:

[0042] The Cordyceps sinensis culture medium used in this embodiment contains 0.1% N-acetylglucosamine.

[0043] 1. Preparation of Cordyceps sinensis bud spores

[0044] The culture medium for culturing budding spores was PPDA medium (by mass fraction: 2% glucose, 20% potato juice, 1% peptone, 0.3% KH₂PO₄, 0.15% MgSO₄·7H₂O, and 0.002% VB₁, with the remainder being H₂O (natural pH)), with 0.1% N-acetylglucosamine added. After accurate molecular identification, the conidia of *Cordyceps sinensis* were inoculated into the above PPDA medium and cultured on a shaker (120 rpm, 12℃) for 60 days. The cultured *Cordyceps sinensis* broth (containing approximately 10 budding spores and mycelia per milliliter) was then cultured. 6 (Each budding spore) is used to infect the body surface of first-instar larvae of the small golden pheasant.

[0045] Meanwhile, *Cordyceps sinensis* was cultured for 60 days in PPDA medium (2% glucose, 20% potato juice, 1% peptone, 0.3% KH2PO4, 0.15% MgSO4·7H2O, and 0.002% VB1, with the remainder being H2O (natural pH)) without the addition of acetylglucosamine in a shaker (120 rpm, 12℃). The cultured *Cordyceps sinensis* spores (containing approximately 10 spores and hyphae per milliliter) were then cultured. 6 (Each budding spore) is used to infect the body surface of first-instar larvae of the small golden pheasant.

[0046] 2. Rearing of small golden bat moth larvae

[0047] By feeding the larvae of the ghost moth with a mixture of carrots, Polygonum bulbiferum, and Potentilla chinensis in a mass ratio of 1:1:1, healthy 1st instar larvae of the ghost moth were obtained for use in infecting the ghost moth.

[0048] 3. Surface infection

[0049] The Cordyceps sinensis mycelium solutions obtained from the two culture media (containing approximately 10 spores and hyphae per milliliter) 6 (1 budding spore) diluted with sterile water to approximately 10 ppm. 5 10 budding spores 5 Add budding spores to 100 grams of sterile coconut coir (moisture content approximately 65%), then mix the budding spore-containing coconut coir with 100 grams of ghost moth larvae feed (Polygonum bulbiferum). Place approximately 500 insect eggs on top of the feed-mixed coconut coir. After hatching, the larvae are infected by the liquid Cordyceps sinensis cultured using the above method. The infected ghost moth larvae are then reared at 11-16℃ (feeding them a mixture of carrots, Polygonum bulbiferum, and Potentilla chinensis in a 1:1:1 mass ratio).

[0050] 4. Detection of bacteria carried by ghost moth larvae infected on their body surface

[0051] Three months after infection on the body surface, about 2-5 microliters of hemolymph were collected from the abdomen of the larvae using a microneedle (0.06 mm in diameter). After staining with Calcofluor White (Sigma), the morphology of the fungus was observed under a microscope, and a large number of budding spores could be observed. Three months after 3 months of external infection of *Cordyceps sinensis* larvae cultured on PPDA medium containing 0.1% acetylglucosamine, the bacterial carrier rate of *Cordyceps sinensis* strain Ophiocordyceps sinensis QHYS2022-3 from Yushu or Ophiocordyceps sinensis strain Ophiocordyceps sinensis XZNQ2206 from Nagqu reached 100%. Under the same conditions, the bacterial carrier rate of *Cordyceps sinensis* strain Ophiocordyceps sinensis GS-MQ from Gansu was approximately 42% after 3 months of external infection of *Cordyceps sinensis* larvae. The bacterial carrier rate of *Cordyceps sinensis* strain Ophiocordyceps sinensis GS-MQ from Gansu cultured on PPDA medium without acetylglucosamine was zero after 3 months of external infection of *Cordyceps sinensis* larvae.

[0052] 5. Sub-entity induction

[0053] Ghost moth larvae infected with Cordyceps sinensis strains from Yushu or Nagqu, cultured in PPDA medium containing 0.1% acetylglucosamine, underwent a 5-7 month growth process, transforming from live larvae into mummified larvae. The heads of the mummified larvae split open, and fruiting bodies emerged from the split opening. The mummification rate was 55.5% for larvae infected with the Yushu strain, 53.5% for those infected with the Nagqu strain, and 10.5% for those infected with the Gansu strain. Artificially cultured Cordyceps sinensis, exhibiting morphology identical to wild Cordyceps sinensis, showed a fruiting body occurrence rate of 94.5% for the Yushu strain, 92.5% for the Nagqu strain, and 12.5% ​​for the Gansu strain. Ghost moth larvae infected with Cordyceps sinensis strains from Yushu, Nagqu, or Gansu, cultured in PPDA medium without acetylglucosamine, had a mummification rate of zero because they did not carry the Cordyceps sinensis fungus.

[0054] Example 3:

[0055] The Cordyceps sinensis culture medium used in this embodiment contains 0.5% acetylglucosamine.

[0056] 1. Preparation of Cordyceps sinensis bud spores

[0057] The culture medium for culturing budding spores was PPDA medium (by mass fraction: 2% glucose, 20% potato juice, 1% peptone, 0.3% KH₂PO₄, 0.15% MgSO₄·7H₂O, and 0.002% VB₁, with the remainder being H₂O (natural pH)), with 0.5% N-acetylglucosamine added. After accurate molecular identification, the conidia of *Cordyceps sinensis* were inoculated into the above PPDA medium and cultured on a shaker (120 rpm, 12°C) for 60 days. The cultured *Cordyceps sinensis* broth (containing approximately 10 budding spores and mycelia per milliliter) was then cultured. 6 (Each budding spore) is used to infect the body surface of first-instar larvae of the small golden pheasant.

[0058] Meanwhile, *Cordyceps sinensis* was cultured for 60 days in PPDA medium (2% glucose, 20% potato juice, 1% peptone, 0.3% KH2PO4, 0.15% MgSO4·7H2O, and 0.002% VB1, with the remainder being H2O (natural pH)) without the addition of acetylglucosamine in a shaker (120 rpm, 12℃). The cultured *Cordyceps sinensis* spores (containing approximately 10 spores and hyphae per milliliter) were then cultured. 6 (Each budding spore) is used to infect the body surface of first-instar larvae of the small golden pheasant.

[0059] 2. Rearing of small golden bat moth larvae

[0060] By feeding the larvae of the ghost moth with a mixture of carrots, Polygonum bulbiferum, and Potentilla chinensis in a mass ratio of 1:1:1, healthy 1st instar larvae of the ghost moth were obtained for use in infecting the ghost moth.

[0061] 3. Surface infection

[0062] The Cordyceps sinensis mycelium solutions obtained from the two culture media (containing approximately 10 spores and hyphae per milliliter) 6 (1 budding spore) diluted with sterile water to approximately 10 ppm. 5 10 budding spores 5 Add budding spores to 100g of sterile coconut coir (moisture content approximately 65%), then mix the budding spore-containing coconut coir with 100g of ghost moth larvae feed (Polygonum bulbiferum). Place approximately 500 eggs on top of the feed-mixed coconut coir. After hatching, the larvae are infected by the Cordyceps sinensis fungus cultured using the above method. Infected ghost moth larvae are then reared at 11-16℃ (feeding them a mixture of carrots, Polygonum bulbiferum, and Potentilla chinensis in a 1:1:1 mass ratio).

[0063] 4. Detection of bacteria carried by ghost moth larvae infected on their body surface

[0064] Three months after infection on the body surface, about 2-5 microliters of hemolymph were collected from the abdomen of the larvae using a microneedle (0.06 mm in diameter). After staining with Calcofluor White (Sigma), the morphology of the fungus was observed under a microscope, and a large number of budding spores could be observed. Three months after larvae of the Cordyceps sinensis strain Ophiocordyceps sinensis QHYS2022-3 (Yushu strain) or Ophiocordyceps sinensis XZNQ2206 (Nagqu strain) were infected with the skin of *Bombyx mori* larvae, the bacterial carrier rate of the larvae reached 100%. Under the same conditions, the bacterial carrier rate of the Cordyceps sinensis strain Ophiocordyceps sinensis GS-MQ (Gansu strain) was approximately 36% after three months of infection with *Bombyx mori* larvae. The bacterial carrier rate of the Cordyceps sinensis strain Ophiocordyceps sinensis GS-MQ (Yushu strain), Nagqu strain, or Gansu strain cultured on PPDA medium without acetylglucosamine was zero after three months of infection with *Bombyx mori* larvae.

[0065] 5. Sub-entity induction

[0066] Ghost moth larvae infected with Cordyceps sinensis strains from Yushu or Nagqu, cultured in PPDA medium containing 0.5% acetylglucosamine, underwent a growth process of 5-7 months, transforming from live larvae into mummified larvae. The heads of the mummified larvae split open, and fruiting bodies emerged from the split opening. The mummification rate was 50.5% for larvae infected with the Yushu strain, 47.5% for those infected with the Nagqu strain, and 7.5% for those infected with the Gansu strain. Artificially cultured Cordyceps sinensis, exhibiting morphology identical to wild Cordyceps sinensis, showed a fruiting body occurrence rate of 89.5% for the Yushu strain, 90.4% for the Nagqu strain, and 10.5% for the Gansu strain. Ghost moth larvae infected with Cordyceps sinensis strains from Yushu or Nagqu, cultured in PPDA medium without acetylglucosamine, had a mummification rate of zero because they did not carry the Cordyceps sinensis fungus.

Claims

1. Application of N-acetylglucosamine in promoting infection of Cordyceps sinensis in ghost moth larvae, inducing Cordyceps sinensis to grow fruiting bodies in ghost moth larvae, and increasing the rate of ghost moth larvae becoming mushy.

2. The application according to claim 1, characterized in that, The N-acetylglucosamine is prepared by culturing Cordyceps sinensis in a culture medium and then using the bacterial solution to infect ghost moth larvae.

3. The application according to claim 1, characterized in that, The concentration of the N-acetylglucosamine is 0.01-1% by mass, preferably 0.01-0.5% by mass.

4. The application according to claim 1, characterized in that, The Cordyceps sinensis fungus mentioned is Ophiocordyceps sinensis QHYS2022-3 or Ophiocordyceps sinensis XZNQ2206. The preservation number of Ophiocordyceps sinensis QHYS2022-3 is GDMCC No. 65577; the preservation number of Ophiocordyceps sinensis XZNQ2206 is GDMCC No. 65578.

5. The application according to claim 1, characterized in that, The bat moth larvae mentioned are small golden bat moth larvae, preferably 1st to 5th instar larvae.

6. A method for artificially cultivating a new strain of Cordyceps sinensis, characterized in that, Includes the following steps: 1) Add 0.01-1% N-acetylglucosamine to the liquid culture medium of Cordyceps sinensis, and then inoculate Cordyceps sinensis for culture to obtain a mixture of budding spores and mycelia. 2) Infect the larvae of the ghost moth with a mixed liquid, then culture them to obtain Cordyceps sinensis.

7. The method according to claim 6, characterized in that, The Cordyceps sinensis fungus mentioned is Ophiocordyceps sinensis QHYS2022-3 or Ophiocordyceps sinensis XZNQ2206. The preservation number of Ophiocordyceps sinensis QHYS2022-3 is GDMCC No. 65577; the preservation number of Ophiocordyceps sinensis XZNQ2206 is GDMCC No. 65578.

8. The method according to claim 6, characterized in that, The bat moth larvae mentioned are small golden bat moth larvae, preferably 1st to 5th instar larvae.

9. The method according to claim 6, characterized in that, The mixture is combined with sterile coconut coir and then used to infect the skin of the ghost moth larvae.

10. Cordyceps sinensis QHYS2022-3, accession number: GDMCC No.65577; or Cordyceps sinensis XZNQ2206, accession number: GDMCC No.65578.