Anti-stress composition and use thereof

Abstract

An anti-stress composition, comprising 2-methyl-2-butenal as an active ingredient. Dosage forms of the composition include an ointment, a pour-on agent, and a spray. The composition is used for combating stress in ruminants, poultry, and equine animals. The stress includes weaning stress, transport stress, vaccine injection stress, heat stress, group mixing stress, fighting stress, noise stress, and oxidative stress.

Description

An anti-stress composition and its application

[0001] Relevant publicly available cross-references

[0002] This disclosure claims priority to Chinese Patent 2024106592285, filed on May 27, 2024, entitled “An Anti-Stress Composition and Its Application,” the entire contents of which are incorporated herein by reference. Technical Field

[0003] This disclosure belongs to the field of aquaculture technology and relates to an anti-stress composition and its application. Background Technology

[0004] In ruminant farming, management procedures such as weaning, long-distance transportation, and necessary medical procedures like vaccinations, while crucial for ensuring animal health and production safety, inevitably impose significant physiological and psychological stress on animals, a phenomenon known as stress response. These stressful events often lead to decreased appetite, slower daily weight gain, and weakened immunity, increasing disease risk and mortality. For the dairy industry, they also significantly reduce milk production, causing economic losses. Therefore, exploring and developing a highly effective and safe anti-stress composition is crucial, aiming to mitigate the adverse effects of stress by regulating animal physiological functions, thereby maintaining and improving the overall production efficiency and economic returns of the farming industry.

[0005] In the broader context of livestock and poultry farming, vaccination is undeniably crucial as an effective means of controlling and preventing various infectious diseases. However, the pain and physiological stress associated with this process, such as temporary loss of appetite and growth inhibition, can sometimes weaken the protective effect of vaccines, and in extreme cases, may even lead to direct animal losses. Therefore, developing an innovative anti-stress drug composition specifically designed to alleviate discomfort and stress after vaccination is essential. This would not only ensure the smooth implementation of vaccination programs but also maximize the immunizing effect of vaccines, providing strong technical support for livestock and poultry health and the sustainable development of the farming industry.

[0006] In particular, frequent transportation needs, especially the transfer of racehorses and sport horses, constitute a significant stressor in high-value equine farming. Prolonged or improper transportation not only directly affects a horse's food intake and weight gain but can also cause serious stress-related illnesses such as transport fever, digestive disorders, and even a higher risk of death. These severely hinder a horse's recovery from competitive conditions, training progress, and ultimately, competition performance. Therefore, designing and applying an anti-stress composition specifically tailored for equines to effectively alleviate transportation stress and maintain good physical and psychological balance is of immeasurable value for improving the competitive level of the equine industry, shortening recovery periods, and increasing economic benefits.

[0007] It is evident that developing effective stress-resistance strategies is crucial for the health, welfare, and economic prospects of the entire aquaculture industry. Summary of the Invention

[0008] The purpose of this disclosure is to provide an anti-stress composition with a simple preparation method and significant effects, addressing the aforementioned problems in the prior art.

[0009] The objective of this disclosure can be achieved through the following technical solution: an anti-stress composition, wherein the active ingredient of the composition is 2-methyl-2-butenal, and the composition dosage form includes one of ointment, pourable and spray.

[0010] In one of the above-mentioned anti-stress compositions, the content of 2-methyl-2-butenal in the composition is 0.01% to 50%.

[0011] In one of the above-mentioned anti-stress compositions, the content of 2-methyl-2-butenal in the composition is 0.1% to 30%.

[0012] This disclosure uses 2-methyl-2-butenal as the core component of an anti-stress composition. Through its chemical reactivity or as a precursor of signaling molecules, it indirectly participates in signal transduction pathways in animals, helping to regulate physiological changes caused by stress, such as immune responses and neuroendocrine activity. Considering that stress responses are often accompanied by oxidative stress and inflammatory responses, 2-methyl-2-butenal has antioxidant or anti-inflammatory properties, which can alleviate these negative effects and protect cells from damage. In addition, the volatile organic solvents of this disclosure can affect the behavior and emotional state of animals. 2-methyl-2-butenal may help animals reduce anxiety or tension through its odor or by acting directly on the nervous system, thereby achieving an anti-stress effect. It may indirectly help animals better cope with various stressors by promoting their adaptability to environmental changes, such as improving sleep quality, increasing appetite, or maintaining a normal metabolic rate.

[0013] In one of the above-mentioned anti-stress compositions, the ointment contains excipients, wherein the excipients include at least one selected from beeswax, lanolin, cetearyl wax, petrolatum, ethylene-vinyl acetate copolymer, agar, resin, paraffin wax, and oils.

[0014] In the aforementioned anti-stress composition, the excipient is beeswax.

[0015] In the aforementioned anti-stress composition, the pourable or sprayable agent contains an organic solvent, which includes at least one of alcohol solvents and ether solvents.

[0016] In the aforementioned anti-stress composition, the alcohol solvent includes at least one selected from ethanol, 1,2-propanediol, 1,3-propanediol, isopropanol, glycerol, ethylene glycol, benzyl alcohol, phenethyl alcohol, propanol, pentanol, hexanol, octanol, and dipropylene glycol.

[0017] In the aforementioned anti-stress composition, the alcohol solvent includes at least one of ethanol and isopropanol.

[0018] In the aforementioned anti-stress composition, the ether solvent includes at least one of diethylene glycol ethyl ether, diethylene glycol monobutyl ether, methyl tert-butyl ether, ethylene glycol monomethyl ether, ethylene glycol monobutyl ether, isosorbide dimethyl ether, propylene glycol methyl ether, propylene glycol ethyl ether, methyl cyclohexyl ether, and diethylene glycol butyl ether acetate.

[0019] In the aforementioned anti-stress composition, the ether solvent includes at least one of diethylene glycol ethyl ether and methyl tert-butyl ether.

[0020] In the aforementioned anti-stress composition, the organic solvent contained in the pouring agent includes at least one of isopropanol and diethylene glycol ethyl ether.

[0021] In the aforementioned anti-stress composition, the spray contains at least one organic solvent selected from ethanol and methyl tert-butyl ether.

[0022] This disclosure also provides the use of the above-described anti-stress composition in an anti-stress product for at least one of ruminants, poultry, and equines.

[0023] This disclosure also provides the use of the above-described anti-stress composition in at least one of A1) to A3), characterized in that A1) to A3) are as follows:

[0024] A1) Anti-stress products for cattle or sheep;

[0025] A2) Anti-stress products for chickens or ducks;

[0026] A3), Anti-stress products for horses.

[0027] This disclosure also provides the use of the above-described anti-stress composition in the preparation of products for at least one of the following stresses: weaning stress, transportation stress, vaccination stress, heat stress, group stress, fighting stress, noise stress, and oxidative stress.

[0028] In some alternative embodiments, the anti-stress composition is used to prepare products for at least one of weaning stress, transportation stress, and vaccination stress.

[0029] Compared with the prior art, the beneficial effects of this disclosure include:

[0030] 1. This disclosure uses 2-methyl-2-butenal as the core component of the anti-stress composition based on its potential physiological regulatory effects, which help to reduce stress response. Moreover, this disclosure offers diverse dosage forms: to meet the needs of different application scenarios and animal species, the composition is designed as a solid balm, a pourable, or a spray, etc. This flexibility improves ease of use and targeting. For example, solid balms are suitable for long-term continuous release and are convenient for use in horse transportation; sprays and pourables are suitable for rapid absorption and large-area coverage.

[0031] 2. This disclosure demonstrates the cross-species applicability and broad-spectrum anti-stress effects of the composition in applications ranging from ruminants to equines and even to a wider range of livestock and poultry farming, particularly in significantly reducing the negative effects of stress before and after critical management procedures such as vaccination and long-distance transport.

[0032] 3. This disclosure describes in detail the specific formulation ratios and preparation steps for each dosage form, emphasizing the simplicity and operability of the preparation methods, which is conducive to large-scale production and widespread application.

[0033] 4. By reducing stress, this composition can not only promote animal growth and development, improve feed conversion rate and production performance, but also enhance animal immunity, reduce disease incidence, and have a positive impact on dairy production and equine athletic performance, thereby achieving a win-win situation for economic benefits and animal welfare. Detailed Implementation

[0034] To make the objectives, technical solutions, and advantages of the embodiments of this disclosure clearer, the technical solutions in the embodiments of this disclosure will be clearly and completely described below. Where specific conditions are not specified in the embodiments, conventional conditions or conditions recommended by the manufacturer shall apply. Reagents or instruments whose manufacturers are not specified are all conventional products that can be purchased commercially.

[0035] First, this disclosure provides an anti-stress composition, the main active ingredient of which is 2-methyl-2-butenal, and the dosage form can be an ointment, a pourable, or a spray, etc.

[0036] Through a series of creative efforts, the above-mentioned anti-stress composition disclosed herein can effectively reduce stress stimuli such as weaning, transportation, and vaccination. These stress stimuli usually lead to negative effects such as decreased animal feed intake, decreased daily weight gain, increased mortality, or decreased milk production, thus greatly improving the production efficiency and animal welfare of the livestock industry.

[0037] In some embodiments, each 100g of the anti-stress composition may include 0.01g, 0.1g, 1g, 2g, 4g, 5g, 6g, 8g, 10g, 12g, 14g, 16g, 18g, 20g, 22g, 24g, 26g, 28g, 30g, 32g, 34g, 35g, 36g, 37g, 38g, 39g, 40g, 41g, 42g, 43g, 44g, 45g, 46g, 47g, 48g, 49g, or 50g of 2-methyl-2-butenal.

[0038] In some preferred embodiments, each 100g of the anti-stress composition may include 0.1 to 30g of 2-methyl-2-butenal.

[0039] The "excipients" in this article can be selected from existing common ointment excipients, which can be obtained through existing preparation methods or purchased.

[0040] In some alternative embodiments, the excipient may include at least one of beeswax, lanolin, cetearyl wax, petrolatum, ethylene-vinyl acetate copolymer, agar, resin, paraffin wax, and oils.

[0041] In some typical implementations, the excipient may include beeswax.

[0042] The term "alcohol solvent" as used herein may be selected from existing known alcohol solvents. In some alternative embodiments, the alcohol solvent may include at least one of ethanol, 1,2-propanediol, 1,3-propanediol, isopropanol, glycerol, ethylene glycol, benzyl alcohol, phenethyl alcohol, propanol, pentanol, hexanol, octanol, and dipropylene glycol.

[0043] In some typical embodiments, the alcohol solvent may include at least one of ethanol and isopropanol.

[0044] The term "ether solvent" as used herein may be selected from existing known ether solvents. In some alternative embodiments, the ether solvent may include at least one of diethylene glycol ethyl ether, diethylene glycol monobutyl ether, methyl tert-butyl ether, ethylene glycol monomethyl ether, ethylene glycol monobutyl ether, isosorbide dimethyl ether, propylene glycol methyl ether, propylene glycol ethyl ether, methyl cyclohexyl ether, and diethylene glycol butyl ether acetate.

[0045] In some typical embodiments, the ether solvent may include at least one of diethylene glycol ethyl ether and methyl tert-butyl ether.

[0046] In some alternative embodiments, the repellent contains at least one of isopropanol and diethylene glycol ethyl ether.

[0047] In some alternative embodiments, the spray contains at least one of ethanol and methyl tert-butyl ether as an organic solvent.

[0048] Furthermore, this disclosure also provides the use of the anti-stress compositions as described in any of the foregoing embodiments in the preparation of anti-stress products for ruminants, poultry, or equines.

[0049] In some alternative embodiments, the anti-stress composition can be used to prepare anti-stress products for cattle, sheep, chickens, ducks, or horses.

[0050] The “stress response” described herein may, but is not limited to, include at least one of the following stresses: weaning stress, transportation stress, vaccination stress, heat stress, group mixing stress, fighting stress, noise stress, and oxidative stress.

[0051] In some typical implementations, "stress response" includes at least one of weaning stress, transportation stress, and vaccination stress.

[0052] The features and performance of this disclosure will be further described in detail below with reference to embodiments.

[0053] Example 1:

[0054] S1. Prepare the following raw materials according to the following mass proportions: 0.1 parts 2-methyl-2-butenal, 99.9 parts beeswax (excipient).

[0055] S2. After heating and melting beeswax, add 2-methyl-2-butenal and stir. Finally, cool and solidify to obtain a solid perfume.

[0056] Example 2:

[0057] S1. Prepare the following raw materials according to the following mass proportions: 10 parts 2-methyl-2-butenal, alcohol solvent (50 parts isopropanol), and ether solvent (49 parts diethylene glycol ethyl ether).

[0058] S2. Mix isopropanol and diethylene glycol ethyl ether evenly, then add 2-methyl-2-butenal and stir to obtain the pouring agent.

[0059] Example 3:

[0060] S1. Prepare the following raw materials according to the following mass proportions: 30 parts 2-methyl-2-butenal, alcohol solvent (50 parts ethanol), and ether solvent (20 parts methyl tert-butyl ether).

[0061] S2. Mix ethanol and diethylene glycol ethyl ether evenly, then add 2-methyl-2-butenal and stir to obtain a spray.

[0062] Example 4:

[0063] S1. Prepare the following raw materials according to the following mass proportions: 40 parts 2-methyl-2-butenal, ether solvent (60 parts diethylene glycol ethyl ether).

[0064] S2. Add diethylene glycol ethyl ether to a mixing tank, then add 2-methyl-2-butenal and stir to obtain a pouring agent.

[0065] Effects of anti-stress compositions on weaning stress in calves and lambs:

[0066] To further investigate the effects of rabbit pheromones on weaning stress in calves and lambs, a controlled experiment was designed. Twenty healthy calves (all in their second month after birth) and 30 lambs (all in their first month after birth) of similar weight were selected and randomly divided into two groups: an experimental group and a control group, with each group containing 10 calves and 15 lambs.

[0067] 1. Pre-weaning preparation: Both groups of calves and lambs underwent routine health checks and feeding management before weaning. Starting one day before weaning, the experimental group animals were treated with the solid balm from Example 1, which was hung 1.5 meters above the ground in each pen of the nursery. The control group animals received the same solid balm base but without rabbit pheromones.

[0068] 2. Weaning process: Both groups of animals were weaned at the same time, i.e., breastfeeding was suddenly stopped, and solid feed and water were introduced. During the weaning process, physiological indicators such as feed intake, water intake, and daily weight gain were recorded, and their behavioral performance (such as anxiety and restlessness) was observed.

[0069] 3. Post-weaning observation: For one week after weaning, the feed intake, water consumption, and weight changes of both groups of animals were continuously monitored, and their health status was observed. In addition, saliva samples from the animals on the second day after weaning were tested for stress hormone levels (such as cortisol).

[0070] Table 1 Summary of Animal Physiological Indicators

[0071] The data above show that the experimental group of calves and lambs had higher feed intake, water consumption, and weight gain after weaning than the control group, while their cortisol levels were lower. This indicates that rabbit pheromones can effectively alleviate weaning stress in calves and lambs, promote their feed intake and growth, and reduce stress hormone levels. During weaning, the experimental group animals also exhibited more stable behavior and significantly reduced anxiety and restlessness. They adapted to solid feed and water more quickly, reducing digestive discomfort and nutritional deficiencies caused by weaning. Furthermore, saliva sample analysis revealed that the cortisol levels in the experimental group were significantly lower than those in the control group. Cortisol is a stress hormone, and its reduced level further confirms the alleviating effect of rabbit pheromones on weaning stress. Therefore, this example details the effect of rabbit pheromones on weaning stress in calves and lambs and verifies its anti-stress effect through experimental data.

[0072] Effects of anti-stress compositions on transport stress in beef cattle and sheep:

[0073] To thoroughly evaluate the effects of rabbit pheromones on stress responses in beef cattle and sheep during transportation, a controlled experiment was designed. Twenty healthy adult beef cattle of similar weight and 30 adult sheep were selected as subjects and randomly divided into two groups: an experimental group and a control group, with each group containing 10 beef cattle and 15 sheep.

[0074] 1. Pre-transport preparation: Both groups of animals underwent routine health checks and feeding management before transportation. One hour before transportation, the experimental group animals were given the application solution from Example 2 by pouring 5 ml (cattle) and 3 ml (sheep) onto their shoulder blades. The control group animals were given the same volume of pure solvent solution (without rabbit pheromones) poured onto their shoulder blades.

[0075] 2. Transportation process: The two groups of animals were transported over a long distance (24 hours) under the same transportation conditions.

[0076] 3. Post-transport observation: After transportation, the animals were immediately transferred to the breeding farm, and their feed intake, daily weight gain, mortality rate, and other indicators were observed and recorded over the next week. Simultaneously, we also tested the cortisol (a stress hormone) levels in saliva samples from the animals on the first day.

[0077] Table 2 Summary of Animal Physiological Indicators

[0078] The data above show that the experimental group of beef cattle and sheep had higher feed intake and daily weight gain after transportation than the control group, while their mortality rate and cortisol levels were lower. This indicates that rabbit pheromones can effectively alleviate the stress response of beef cattle and sheep during transportation, increase their feed intake and daily weight gain, reduce stress-induced mortality, and lower cortisol levels in saliva. This example details the effects of rabbit pheromones on transportation stress in beef cattle and sheep and verifies its anti-stress effect through experimental data.

[0079] Effects of anti-stress composition on milk production in dairy cows and goats after vaccine injection:

[0080] To evaluate the effect of rabbit pheromones on milk production in dairy cows and goats after vaccination (foot-and-mouth disease vaccine), a controlled experiment was conducted. Twenty dairy cows (all multiparous and in good health) and 30 dairy goats (all multiparous and in good health) were selected and randomly divided into two groups: an experimental group and a control group, with each group containing 10 dairy cows and 15 dairy goats.

[0081] The experimental group of dairy cows and sheep began treatment one day prior to vaccination by evenly spraying 3 ml (cows) and 2 ml (sheep) of the spray from Example 3 onto their shoulder blades and necks. The control group of dairy cows and sheep received the same amount of pure solvent solution (without rabbit pheromones) at the same time points. Milk production in both groups was recorded daily during the experiment, and the data were statistically analyzed. The experiment lasted for one month to fully observe the long-term effects of rabbit pheromones on milk production.

[0082] Table 3 Milk Production Statistics

[0083] The data above show that the milk production of both cows and sheep in the experimental group was significantly higher than that in the control group. This indicates that rabbit pheromones can effectively alleviate the stress response of cows and sheep after vaccination, thereby increasing their milk production. Furthermore, it was observed that the experimental group animals exhibited more stable behavior, improved appetite and energy levels after vaccination, further confirming the positive impact of rabbit pheromones on the stress response of ruminants. This example demonstrates the positive effect of rabbit pheromones on milk production in cows and sheep after vaccination, providing a new anti-stress strategy for the livestock industry.

[0084] Effects of anti-stress composition on stress in broiler ducks and chickens after vaccine injection:

[0085] To evaluate the effects of rabbit pheromones on stress in broiler ducks and chickens after vaccination, a controlled experiment was conducted. Forty healthy broiler chickens and forty healthy ducks were selected and randomly divided into two groups: an experimental group and a control group, with each group containing 20 broiler chickens and 20 ducks.

[0086] 1. Pre-experiment preparation: Starting one day before vaccination, the experimental group animals were treated with the solid balm from Example 1, which was suspended 1.5 meters above the ground in their enclosures. The control group animals received the same solid balm with the same matrix but without rabbit pheromones.

[0087] 2. Vaccination: All experimental animals received routine vaccinations within the same time period.

[0088] 3. Data recording: Observe and record their feed intake, daily weight gain, mortality rate and other indicators over the next week.

[0089] Table 4. Statistics on feed intake, daily weight gain, and mortality rate

[0090] The data above show that the experimental group of broiler chickens and ducks had higher feed intake and daily weight gain than the control group after vaccination, while their mortality rate was lower. This indicates that the anti-stress composition provided in this disclosure can effectively alleviate the stress response of broiler chickens and ducks after vaccination, increase their feed intake and growth rate, and reduce mortality.

[0091] Effects of anti-stress compositions on transport stress in horses and donkeys:

[0092] To thoroughly evaluate the effects of rabbit pheromones on stress responses in horses and donkeys during transportation, a controlled experiment was designed. Twenty adult horses of similar weight and in good health, and twenty adult donkeys were selected as subjects and randomly divided into two groups: an experimental group and a control group, with each group containing 10 horses and 10 donkeys.

[0093] Experimental methods:

[0094] 1. Pre-transport preparation: Both groups of animals underwent routine health checks and feeding management before transportation. One hour before transportation, the experimental group animals were given the application agent from Example 2 by pouring 5 ml onto their necks, while the control group animals were given the same volume of pure solvent solution (without rabbit pheromones) poured onto their necks. Pre-transport weights were recorded.

[0095] 2. Transportation process: The two groups of animals were transported over a long distance (24 hours) under the same transportation conditions.

[0096] 3. Post-transport observation: After transportation, we immediately transferred the animals to the breeding farm and observed and recorded their feed intake, daily weight gain, and mortality rate for the next week. We also calculated the post-transport weight loss rate as (post-transport weight - initial weight) / initial weight × 100%.

[0097] Table 5. Statistics on feed intake, daily weight gain, and mortality rate.

[0098] The data above show that rabbit pheromones have a significant positive impact on feed intake, daily weight gain, and post-transport weight loss in horses after transport stress, but no significant effect on donkeys. However, for donkeys, there were no significant differences between the experimental and control groups in terms of feed intake, daily weight gain, mortality rate, and post-transport weight loss. This may be because donkeys have low sensitivity to rabbit pheromones, or that the effect of rabbit pheromones at this concentration on donkeys is not significant.

[0099] Comparative experiment: The effects of high concentrations of rabbit pheromones on transport stress in horses and donkeys.

[0100] To thoroughly evaluate the effects of high concentrations of rabbit pheromones on stress responses in horses and donkeys during transport, we designed a controlled experiment. Twenty adult horses and twenty adult donkeys were selected as subjects and randomly divided into two groups: an experimental group and a control group, with each group containing 10 horses and 10 donkeys.

[0101] 1. Pre-transport preparation: Both groups of animals underwent routine health checks and feeding management before transportation. One hour before transportation, the experimental group animals were given the application agent from Example 4 by pouring 5 ml onto their necks, while the control group animals were given the same volume of pure solvent solution (without rabbit pheromones) poured onto their necks. Pre-transport weights were recorded.

[0102] 2. Transportation process: The two groups of animals were transported over a long distance (24 hours) under the same transportation conditions.

[0103] 3. Post-transport observation: Immediately after transportation, transfer the animals to the breeding farm and observe and record their feed intake, daily weight gain, and mortality rate for the following week. Calculate the post-transport weight loss rate: (post-transport weight - initial weight) / initial weight × 100%.

[0104] The following is a summary table of the experimental results:

[0105] Table 6. Statistics on feed intake, daily weight gain, and mortality rate

[0106] The data above shows that high concentrations of rabbit pheromones have a stronger positive effect on horses than low concentrations, suggesting that the anti-stress effect in horses may increase with increasing concentration. However, high concentrations of rabbit pheromones showed no significant difference in feed intake, daily weight gain, mortality rate, or post-transport weight loss in donkeys compared to low concentrations. This may be because donkeys do not exhibit any anti-stress effect from calming pheromones themselves.

[0107] In summary, this disclosure uses 2-methyl-2-butenal as the core component of the anti-stress composition based on its potential physiological regulatory effects, which helps to reduce stress response. Moreover, this disclosure offers diverse dosage forms: to meet the needs of different application scenarios and animal species, the composition is designed as a solid balm, a pourable, and a spray. This flexibility improves ease of use and targeting. For example, the solid balm is suitable for long-term continuous release, making it convenient for use in horse transportation; while the spray and pourable are suitable for rapid absorption and large-area coverage.

[0108] The embodiments herein cover any points not exhaustively covered by the technical scope of this disclosure, as well as new technical solutions formed by equivalent substitutions of one or more technical features in the embodiments. These are all within the scope of this disclosure. Furthermore, in all listed or unlisted embodiments of this disclosure, each parameter in the same embodiment merely represents an instance (i.e., a feasible solution), and there is no strict coordination or limitation relationship between the parameters. The parameters can be substituted for each other without violating axioms and the claims of this disclosure, unless otherwise stated.

[0109] The technical means disclosed in this disclosure are not limited to those described above, but also include technical solutions composed of any combination of the above technical features. The above descriptions are specific embodiments of this disclosure. It should be noted that those skilled in the art can make various improvements and modifications without departing from the principles of this disclosure, and these improvements and modifications are also considered within the scope of protection of this disclosure.

[0110] The specific embodiments described herein are merely illustrative examples of the spirit of this disclosure. Those skilled in the art to which this disclosure pertains may make various modifications or additions to the described specific embodiments or use similar methods to substitute them, without departing from the spirit of this disclosure or exceeding the scope defined by the appended claims. Industrial applicability

[0111] The anti-stress composition disclosed herein helps alleviate stress responses and has cross-species applicability and a broad-spectrum anti-stress effect, particularly before and after critical management procedures, such as vaccination and long-distance transportation, significantly reducing the negative effects of stress. It can be prepared into various dosage forms, and the preparation method is simple and easy to operate, facilitating large-scale production and widespread application. Furthermore, this anti-stress composition not only promotes animal growth and development, improves feed conversion rate and production performance, but also enhances animal immunity, reduces disease incidence, and has a positive impact on dairy production and equine athletic performance, thus achieving a win-win situation for both economic benefits and animal welfare.

Claims

1. A stress resistant composition, characterized in that, The active ingredient of the composition is 2-methyl-2-butenal, wherein the composition dosage form includes one of a cream, a pour-on, and a spray.

2. The anti-stress composition according to claim 1, characterized by The 2-methyl-2-butenal content in the composition is 0.01% to 50%.

3. The anti-stress composition according to claim 1 or 2, characterized by, The 2-methyl-2-butenal content in the composition is 0.1% to 30%.

4. The anti-stress composition according to any one of claims 1 to 3, characterized in that, The cream contains an excipient, wherein the excipient includes at least one of beeswax, lanolin, spermaceti, vaseline, ethylene-vinyl acetate copolymer, agar, resin, paraffin, and oil.

5. The anti-stress composition according to claim 4, characterized in that, The excipient is beeswax.

6. The anti-stress composition according to any one of claims 1 to 3, characterized in that, The pour-on or the spray contains an organic solvent, wherein the organic solvent includes at least one of an alcohol solvent and an ether solvent.

7. The stress-resistant composition according to claim 6, wherein The alcohol solvent includes at least one of ethanol, 1,2-propanediol, 1,3-propanediol, isopropyl alcohol, glycerol, ethylene glycol, benzyl alcohol, phenethyl alcohol, propyl alcohol, amyl alcohol, hexyl alcohol, octyl alcohol, and dipropylene glycol.

8. The stress-resistant composition of claim 7, wherein, The alcohol solvent includes at least one of ethanol and isopropyl alcohol.

9. The stress-resistant composition of claim 6, wherein, The ether solvent includes at least one of diethylene glycol ethyl ether, diethylene glycol monobutyl ether, methyl tert-butyl ether, ethylene glycol monomethyl ether, ethylene glycol monobutyl ether, isosorbide dimethyl ether, propylene glycol methyl ether, propylene glycol ethyl ether, methylcyclohexyl ether, and diethylene glycol butyl ether acetate.

10. The anti-stress composition according to claim 9, characterized in that, The ether solvent includes at least one of diethylene glycol ethyl ether and methyl tert-butyl ether.

11. The anti-stress composition according to any one of claims 6 to 10, characterized in that, The organic solvent contained in the pour-on includes at least one of isopropyl alcohol and diethylene glycol ethyl ether.

12. The anti-stress composition according to any one of claims 6 to 10, characterized in that, The organic solvent contained in the spray includes at least one of ethanol and methyl tert-butyl ether.

13. Use of the anti-stress composition according to any one of claims 1 to 12 in an anti-stress product for at least one of a ruminant, poultry, and equine.

14. Use of a stress-resistant composition according to any one of claims 1 to 12 in at least one of A1 ) to A3), characterized in that, A1) to A3) are as follows: A1), an anti-stress product for a bovine or ovine; A2), an anti-stress product for a chicken or duck; A3), an anti-stress product for a horse.

15. Use of the anti-stress composition according to any one of claims 1 to 12 in the preparation of a product against at least one of weaning stress, transportation stress, vaccine injection stress, heat stress, mixing stress, fighting stress, noise stress, and oxidative stress.

16. The method of claim 15, wherein the step of determining the location of the mobile device comprises: The anti-stress composition is used in the preparation of a product against at least one of weaning stress, transportation stress, and vaccine injection stress.

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