Cosmetic use of an extract of at least one bacterium of the species staphylococcus epidermidis as a healing agent

Abstract

The present invention relates to the non-therapeutic cosmetic use of an extract of at least one bacterium of the species Staphylococcus epidermidis obtainable by at least one step of culturing at least one bacterium of the species Staphylococcus epidermidis, in the presence of a culture medium comprising i) at least one monosaccharide, ii) at least one peptone of vegetable origin and / or at least one yeast extract, and optionally iii) at least one microelement, as an active healing agent, and / or for promoting and / or accelerating the healing and / or repair and / or regeneration of keratin materials, preferably the skin. The present invention also relates to the non-therapeutic cosmetic use of a biomass extract of at least one bacterial strain of the species Staphylococcus epidermidis selected from the group consisting of bacteria deposited at the CNCM under serial numbers I-5688, I-5689, I-5904, I-5691, I-5692, I-5693, I-5694 and I-5695 as an active healing agent, and / or for promoting and / or accelerating the healing and / or repair and / or regeneration of keratin materials, preferably the skin, and also to a method for cosmetic treatment of keratin materials, employing said extract within a composition, especially a cosmetic composition.

Description

Cosmetic use of an extract of at least one bacterium of the speciesStaphylococcus epidermidisas a healing agent

[0001] The present invention relates to the field of the care of keratin materials, in particular to skin care, more particularly to the healing and regeneration of the skin.

[0002] The present invention relates to the non-therapeutic cosmetic use of an extract of at least one bacterium of the speciesStaphylococcus epidermidisas an active healing agent, and / or for promoting and / or accelerating the healing and / or repair and / or regeneration of keratin materials, preferably the skin, which is obtainable by a particular method. The present invention also relates to the non-therapeutic cosmetic use of an extract of at least one specific bacterial strain of the speciesStaphylococcus epidermidisas an active healing agent, and / or for promoting and / or accelerating the healing and / or repair and / or regeneration of keratin materials, preferably the skin. The present invention also relates to a method for cosmetic treatment of keratin materials, employing said extract within a composition for promoting and / or accelerating the healing and / or repair and / or regeneration of keratin materials, preferably the skin.Prior art

[0003] Human skin is made up of two compartments: a deep compartment, the dermis, and a superficial compartment, the epidermis. The epidermis is in contact with the external environment, and its role is to protect the body from dehydration and external chemical or mechanical aggressions.

[0004] Skin regeneration or healing is an area of intense scientific research aimed at improving repair of the skin following an external aggression or when the barrier function is compromised. For example, the skin barrier may be unbalanced after a cosmetic procedure such as laser treatments and chemical peels, or in the presence of external aggressions such as irritants (cleansing agents) or else mechanical stresses (rubbing, shaving or hair removal), thermal or climatic imbalances (cold, dryness, radiation), xenobiotics (allergens) or internal aggressions such as psychological stress. As a result of such external aggressions, a repair process is triggered, aimed at quickly and completely restoring this barrier. This physiological process depends on complex biological mechanisms involving numerous cellular and molecular interactions.

[0005] There is a need for compositions that promote the healing of the skin, and in particular that accelerate skin healing.

[0006] In parallel, the formulation of environmentally friendly cosmetic products, i.e., products whose design and development take account of environmental issues, is becoming a major preoccupation for contributing to meeting the challenges facing the planet. It is therefore proving essential to propose ingredients that make it possible to respond to these environmental issues.

[0007] In this context, it is important to develop new ingredients and / or active agents that are environmentally conscious and are especially of natural origin, in particular biobased, and / or that are derived from sustainable sources that are not obtained from petrochemistry and / or that are biodegradable and / or the process for the extraction of which requires a low consumption of energy and water in order to provide compositions that enable a reduction in the environmental impact of the products.Disclosure of the invention

[0008] It is an object of the present invention to provide a cosmetic composition meeting all of these requirements.

[0009] Indeed, the inventors have made the surprising discovery that an extract of a bacterium of the speciesStaphylococcus epidermidis,in particular a bacterial extract in the form of a lysate of a bacterial strain CNCM-I 5691 of the speciesStaphylococcus epidermidis, makes it possible to accelerate the migration of keratinocytes, and is therefore useful for the regeneration and / or healing of keratin materials such as the skin, in particular epithelial regeneration and / or healing.Summary of the invention

[0010] A first subject of the present invention is the non-therapeutic cosmetic use of an extract of at least one bacterium of the speciesStaphylococcus epidermidisobtainable, obtained or directly obtained by at least one step of culturing at least one bacterium of the speciesStaphylococcus epidermidis, preferably at least one bacterial strain of the speciesStaphylococcus epidermidisselected from the group consisting of bacteria deposited at the CNCM under serial numbers I-5688, I-5689, I-5904, I-5691, I-5692, I-5693, I-5694 and I-5695, in the presence of a culture medium comprising i) at least one monosaccharide, ii) at least one peptone of vegetable origin and / or at least one yeast extract, and optionally iii) at least one microelement, as an active healing agent, and / or for promoting and / or accelerating the healing and / or repair and / or regeneration of keratin materials, preferably the skin.

[0011] Another subject thereof is the non-therapeutic cosmetic use of a biomass extract of at least one bacterial strain of the speciesStaphylococcus epidermidisselected from the group consisting of bacteria deposited at the CNCM under serial numbers I-5688, I-5689, I-5904, I-5691, I-5692, I-5693, I-5694 and I-5695 as an active healing agent, and / or for promoting and / or accelerating the healing and / or repair and / or regeneration of keratin materials, preferably the skin.

[0012] The present invention also relates to a method for non-therapeutic cosmetic treatment of keratin materials, preferably the skin, which comprises topical application to the keratin materials, preferably the skin, of a composition comprising, in a physiologically acceptable medium, an extract according to the invention, for promoting and / or accelerating the healing and / or repair and / or regeneration of keratin materials, preferably the skin.

[0013] Other features, aspects and advantages of the invention will become apparent on reading the detailed description that follows.Definitions

[0014] The term "keratin materials" is understood to mean the skin of the body or of the face, the lips, the mucous membranes, the eyelashes, the nails, and the hair, of a human being.

[0015] The term "skin" is understood to mean all of the skin of the body and the scalp of a human being and preferably the skin of the face, neckline, neck, arms and forearms, and hands, or even more preferably still the skin of the face (in particular of the forehead, nose, cheeks, lips and chin), neckline and neck.

[0016] The term "physiologically acceptable medium" is understood to denote a medium which is particularly suitable for the application of a composition of the invention to keratin materials, in particular the skin.

[0017] Within the meaning of the present invention, the term "promoting" refers to increasing the effects of the phenomenon. For example, boosting the regeneration of the skin may lead to greater regeneration of the skin than without the use of the extract according to the invention.

[0018] Within the meaning of the present invention, the term "accelerating" refers to accelerating the phenomenon. For example, accelerating the regeneration of the skin may lead to the same amount of regenerated skin, but in less time than without the use of the extract according to the invention.

[0019] Within the meaning of the present invention, the expression "regeneration of keratin materials" or "regeneration of the skin" is understood to mean the innate capacity of living organisms to renew their keratin materials or their skin, by a process of cell renewal, in particular of re-epithelialization of the skin.

[0020] Within the meaning of the present invention, the expression "healing of keratin materials" or "healing of the skin" is understood to mean the innate capacity of living organisms to repair their keratin materials or their skin, by a process of cell migration or migration of skin cells, in particular of migration of keratinocytes and / or fibroblasts.

[0021] In the context of the invention, "healing of keratin materials" or "healing of the skin" may follow a lesion of keratin materials, in particular a skin lesion, or may follow a compromised skin barrier function - for example, during or after cosmetic surgery procedures.

[0022] Advantageously, the healing of the skin is the healing of a skin lesion or a skin wound.

[0023] The term "topical use or application" is understood to mean the use or application at the surface of the keratin materials in question, preferably at the surface of the skin in question.

[0024] Staphylococcus epidermidis, or white staphylococcus, is a human commensal bacterium and part of the mucocutaneous flora of virtually the whole of the human population.

[0025] Staphylococcus epidermidis(S. epidermidis) strain CNCM I-5688 (I-5688) is the strain deposited by L’Oréal with the CNCM on June 3, 2021 under accession number CNCM I-5688 (I-5688).

[0026] Staphylococcus epidermidis(S. epidermidis) strain CNCM I-5689 is the strain deposited by L’Oréal with the CNCM on June 3, 2021 under accession number CNCM I-5689 (I-5689).

[0027] Staphylococcus epidermidis(S. epidermidis) strain CNCM I-5904 is the strain deposited by L’Oréal with the CNCM on September 21, 2022 under accession number CNCM I-5904 (I-5904).

[0028] Staphylococcus epidermidis(S. epidermidis) strain CNCM I-5691 is the strain deposited by L’Oréal with the CNCM on June 3, 2021 under accession number CNCM I-5691 (I-5691).

[0029] Staphylococcus epidermidis(S. epidermidis) strain CNCM I-5692 is the strain deposited by L’Oréal with the CNCM on June 3, 2021 under accession number CNCM I-5692 (I-5692).

[0030] Staphylococcus epidermidis(S. epidermidis) strain CNCM I-5693 is the strain deposited by L’Oréal with the CNCM on June 3, 2021 under accession number CNCM I-5693 (I-5693).

[0031] Staphylococcus epidermidis(S. epidermidis) strain CNCM I-5694 is the strain deposited by L’Oréal with the CNCM on June 7, 2021 under accession number CNCM I-5694 (I-5694).

[0032] Staphylococcus epidermidis(S. epidermidis) strain CNCM I-5695 is the strain deposited by L’Oréal with the CNCM on June 7, 2021 under accession number CNCM I-5695 (I-5695).

[0033] Within the meaning of the present invention, the term "extract of at least one bacterium" refers equally to the culture supernatant of said bacteria, the biomass obtained after culturing said bacteria or else the extracts of the biomass that are obtained by treating this biomass.

[0034] The bacterial extract according to the invention may be in a living, semi-active or inactivated, dead, form; the bacterial extract according to the invention is preferably in an inactivated or dead form.

[0035] Advantageously, said extract is distinct from the culture supernatant of said bacteria.

[0036] Advantageously, said extract of at least one bacterium of the speciesStaphylococcus epidermidisis a lysate of at least one bacterium of the speciesStaphylococcus epidermidis.

[0037] Within the meaning of the present invention, a "lysate" means a material obtained after the alteration of bacterial cells, such as the destruction or breakage of bacterial cells, in particular of the cell walls and / or membranes, by a phenomenon known as cell lysis, thus causing the release of intracellular biological constituents naturally contained in the cells of the microorganism in question.

[0038] Within the meaning of the present invention, the term “lysate” is used without prejudice to denote the entirety of the lysate obtained by lysis of the microorganism in question, or only a fraction thereof.

[0039] The lysate used is thus formed totally or partially from the intracellular biological constituents and constituents of the cell walls and membranes.

[0040] More specifically, it contains the cellular cytoplasmic fraction containing enzymes such as lactic acid dehydrogenase, phosphatases, phosphoketolases, transaldolases and metabolites. For illustrative purposes, the constituents of the cell walls are especially peptidoglycan, murein or mucopeptide and teichoic acid, and the constituents of the cell membranes are composed of glycerophospholipids.

[0041] This cell lysis can be accomplished by various technologies well known to those skilled in the art, such as osmotic shock, thermal shock, ultrasound or centrifugation, preferably thermal shock such as freezing followed by thawing.Detailed description

[0042] A first subject of the present invention is the non-therapeutic cosmetic use of an extract of at least one bacterium of the speciesStaphylococcus epidermidisobtainable, obtained or directly obtained by at least one step of culturing at least one bacterium of the speciesStaphylococcus epidermidis, preferably an extract of at least one bacterial strain of the speciesStaphylococcus epidermidisselected from the group consisting of bacteria deposited at the CNCM under serial numbers I-5688, I-5689, I-5904, I-5691, I-5692, I-5693, I-5694 and I-5695, in the presence of a culture medium comprising i) at least one monosaccharide, ii) at least one peptone of vegetable origin and / or at least one yeast extract, and optionally iii) at least one microelement, as an active healing agent, and / or for promoting and / or accelerating the healing and / or repair and / or regeneration of keratin materials, preferably the skin.

[0043] The extract of at least one bacterium of the speciesStaphylococcus epidermidisaccording to the invention is preferably chosen from i) the culture supernatant of said bacterium, ii) the biomass obtained after culturing said bacterium and iii) the biomass obtained after culturing said bacterium which has undergone a chemical or physical treatment such as a cell lysis treatment and / or a stabilization treatment. Said biomass iii) being preferably in the form of a lysate.

[0044] Preferably, said chemical or physical treatment is chosen from osmotic shock, thermal shock, ultrasound, centrifugation, autoclaving, ultra-high temperature (UHT technique), high-pressure sterilization, freeze drying, gamma radiation, and freezing.

[0045] Preferably, said bacterium of the speciesStaphylococcus epidermidiscultured is a bacterial strain of the speciesStaphylococcus epidermidisselected from the group consisting of bacteria deposited at the CNCM under the serial numbers I-5688, I-5689, I-5904, I-5691, I-5692, I-5693, I-5694 and I-5695, preferably a bacterial strain of the speciesStaphylococcus epidermidisselected from the group consisting of bacteria deposited at the CNCM under serial numbers I-5688, I-5691, I-5904, I-5692, I-5693, I-5694 and I-5695, even more preferably a bacterial strain of the speciesStaphylococcus epidermidisdeposited at the CNCM under the serial number I-5691.

[0046] Advantageously, said culture medium is free of peptone of animal origin, and preferably said culture medium is free of peptone casein.

[0047] Advantageously, said culture medium comprises less than 4 g / L of sodium chloride, preferably less than 1 g / L by weight of sodium chloride, better still less than 0.5 g / L of sodium chloride, or even is free (0 g / L) of sodium chloride.

[0048] Advantageously, said culture medium is distinct from TSB (tryptic soy broth) medium.

[0049] Within the meaning of the present invention, the term "peptone" is understood to mean a product of a hydrolysis reaction of protein-rich food products. This hydrolysis can be chemical or enzymatic. There are three distinct types of protein raw materials for the manufacture of peptones: peptones of animal origin such as acid casein and whey; peptones of vegetable origin such as soybean, cotton, corn, bean and wheat; and peptones of mycelial origin. Besides the origin of the proteins, peptones can be separated according to their type of hydrolysis: chemical hydrolysis typically by hydrochloric acid, then neutralized by sodium hydroxide; enzymatic hydrolysis, using proteolytic enzymes, digestive enzymes such as pepsin, trypsin and pancreatin, or not, such as papain.

[0050] According to the present invention, the peptone used is a peptone of vegetable origin, preferably a peptone of vegetable origin chosen from soybean, cotton, corn, bean or wheat peptones.

[0051] Advantageously, the peptone used is a peptone obtained by enzymatic hydrolysis, preferably using proteolytic enzymes, more preferably using papain.

[0052] According to one preferred embodiment, the peptone used is a soybean papain peptone.

[0053] Preferably, said culture medium comprises in water, preferably in distilled water:

[0054] i. at least one monosaccharide present in the culture medium in an amount ranging from 0.5 to 30 g / L, and

[0055] ii. at least one peptone of vegetable origin present in the culture medium in an amount ranging from 0.5 to 15 g / L, and / or at least one yeast extract present in the culture medium in an amount ranging from 0.5 to 15 g / L, and

[0056] iii. optionally at least one microelement present in the culture medium in an amount ranging from 0.05 to 6 mg / L, and

[0057] iv. optionally at least one calcium salt present in the culture medium in an amount ranging from 0.01 to 1 g / L.

[0058] Preferentially, said culture medium comprises in water, preferably in distilled water:

[0059] i. at least one monosaccharide present in the culture medium in an amount ranging from 0.5 to 7 g / L, and

[0060] ii. at least one peptone of vegetable origin present in the culture medium in an amount ranging from 0.5 to 5 g / L, and / or at least one yeast extract present in the culture medium in an amount ranging from 0.5 to 5 g / L, and

[0061] iii. optionally at least one microelement present in the culture medium in an amount ranging from 0.5 to 6 mg / L, and

[0062] iv. optionally at least one calcium salt present in the culture medium in an amount ranging from 0.01 to 0.1 g / L.

[0063] More preferentially, said culture medium comprises in water, preferably in distilled water:

[0064] i. at least one monosaccharide present in the culture medium in an amount ranging from 0.5 to 7 g / L, said monosaccharide being chosen from glucose, sucrose, and mixtures thereof, and

[0065] ii. at least one peptone of vegetable origin present in the culture medium in an amount ranging from 0.5 to 5 g / L, said peptone being soybean papain peptone, and at least one yeast extract present in the culture medium in an amount ranging from 0.5 to 5 g / L, said yeast extract being an autolytic yeast extract, and

[0066] iii. at least one microelement present in the culture medium in an amount ranging from 0.5 to 6 mg / L, the one or more microelements being chosen from Heller microelements, and

[0067] iv. at least one calcium salt present in the culture medium in an amount ranging from 0.01 to 0.1 g / L, said calcium salt being calcium chloride.

[0068] Even more preferentially, said culture medium comprises in water, preferably in distilled water:

[0069] i. at least one monosaccharide present in the culture medium in an amount ranging from 0.5 to 7 g / L, said monosaccharide being glucose, and

[0070] ii. at least one peptone of vegetable origin present in the culture medium in an amount ranging from 0.5 to 5 g / L, said peptone being soybean papain peptone, and at least one yeast extract present in the culture medium in an amount ranging from 0.5 to 5 g / L, said yeast extract being an autolytic yeast extract, and

[0071] iii. at least one microelement present in the culture medium in an amount ranging from 0.5 to 6 mg / L, the one or more microelements being chosen from Heller microelements, and

[0072] iv. at least one calcium salt present in the culture medium in an amount ranging from 0.01 to 0.1 g / L, said calcium salt being calcium chloride.

[0073] The Heller microelements, whose composition is given below, were described by Heller, Ann Sci. Nat. Biol. Veg. 14:1 223 (1953). They are mixtures of various mineral elements which were recommended by Heller, not for culturing bacteria, but for the nutrition of plant tissues cultivated in vitro. It should be noted here that no attempt has been made to determine whether the Heller microelements are all indispensable or useful for culturing said bacterium. However, it has been found that the Heller microelements used together in combination with the other abovementioned constituents effectively allow the bacteria in question to be cultured.

[0074] The composition of the Heller microelements, per liter of distilled water, is as follows:

[0075] ZnSO4·7 H2O1 gMnSO4·H2O0.076 gCuSO4·5H2O0.003 gKI0.010 gAlCl3.6H2O0.050 gNiCl2.6H2O0.030 g

[0076] The culturing step may be performed at the appropriate temperature suitable for the bacterial species cultured. Generally, this temperature is between 18 and 40°C. The pH of the culture medium is preferably between 5 and 8.

[0077] When the bacterial culture has reached a terminal stage, that is to say in general when the biomass is at the end of exponential growth, said biomass can be separated from the culture medium. Thus, preferably, after the culturing step, a step of separating the bacteria, also called biomass, from the culture medium is carried out. This separation step can be obtained by any conventional technique, preferably by centrifugation, filtration, or coagulation, in particular with an alcohol such as ethanol, isopropanol, isobutanol, and mixtures thereof. More preferably, the separation step is a separation of the bacteria (or biomass) from the culture medium by centrifugation.

[0078] The supernatant fraction of this biomass can also be filtered in a sterile container to remove suspended particles, using for example a 0.2 µm filter, and / or stabilized, preferably by heat treatment, by autoclaving for example between 115°C and 121°C for 15 to 40 minutes. This supernatant fraction may also be transferred aseptically into a sterile container. According to one particular embodiment of the invention, the supernatant fraction thus obtained is used as an active healing agent according to the invention.

[0079] Preferably, after the separation step, a cell lysis step is performed. This cell lysis step can be obtained by any conventional technique well known to those skilled in the art, preferably by osmotic shock, thermal shock, ultrasound, or centrifugation, preferably by thermal shock such as freezing followed by thawing.

[0080] Preferably, after the step of separating the bacteria (or biomass) from the culture medium, a step of stabilizing the bacteria (or biomass) is carried out.

[0081] Preferably, after the cell lysis step, a step of stabilizing the lysate obtained at the end of the cell lysis step is carried out.

[0082] This stabilization step can then be carried out by any method well known to those skilled in the art, preferably by autoclaving, ultra-high temperature (UHT technique), high-pressure sterilization, freeze drying, gamma radiation or freezing, preferentially by autoclaving, even more preferentially by autoclaving carried out at a temperature of between 115°C and 121°C and / or for a time of between 15 and 40 minutes.

[0083] In one preferred embodiment, the stabilization step is carried out by autoclaving carried out at a temperature of between 115°C and 121°C and / or for a time of between 15 and 40 minutes.

[0084] In another preferred embodiment, the stabilization step is carried out by freeze drying.

[0085] A preparation example for an extract which can be used according to the invention is also given in the examples.

[0086] The present invention also relates to the non-therapeutic cosmetic use of a biomass extract of at least one bacterial strain of the speciesStaphylococcus epidermidisselected from the group consisting of bacteria deposited at the CNCM under serial numbers I-5688, I-5689, I-5904, I-5691, I-5692, I-5693, I-5694 and I-5695 as an active healing agent, and / or for promoting and / or accelerating the healing and / or repair and / or regeneration of keratin materials, preferably the skin.

[0087] Advantageously, said extract is a lysate of at least one bacterial strain of the speciesStaphylococcus epidermidisselected from the group consisting of bacteria deposited at the CNCM under serial numbers I-5688, I-5689, I-5904, I-5691, I-5692, I-5693, I-5694 and I-5695.

[0088] Advantageously, said extract is an extract of at least one bacterial strain of the speciesStaphylococcus epidermidisselected from the group consisting of bacteria deposited at the CNCM under serial numbers I-5688, I-5691, I-5904, I-5692, I-5693, I-5694 and I-5695.

[0089] Advantageously, said extract is a lysate of at least one bacterial strain of the speciesStaphylococcus epidermidisselected from the group consisting of bacteria deposited at the CNCM under serial numbers I-5688, I-5691, I-5904, I-5692, I-5693, I-5694 and I-5695.

[0090] More preferably, said extract is an extract of at least one bacterial strain of the speciesStaphylococcus epidermidisdeposited at the CNCM under the serial number I-5691.

[0091] Even more preferably, said extract is a lysate of at least one bacterial strain of the speciesStaphylococcus epidermidisdeposited at the CNCM under the serial number I-5691.

[0092] Advantageously, said extract comprises amino acids; preferably, said amino acids are present in an amount ranging from 20% to 50% by weight relative to the total weight of the dry extract; more preferably, said amino acids are present in an amount ranging from 25% to 45% by weight relative to the total weight of the dry extract.

[0093] The amino acids can be chosen from aspartic acid, threonine, serine, glutamic acid, proline, valine, alanine, cystine, glycine, methionine, isoleucine, leucine, tyrosine, phenylalanine, lysine, arginine, histidine, and mixtures thereof.

[0094] For the purposes of the present invention, the term "amino acids" is understood to mean both free amino acids and those contained in proteins.

[0095] Advantageously, said extract comprises lipids; preferably, said lipids are present in an amount ranging from 1% to 10% by weight relative to the weight of the dry extract; more preferably, said lipids are present in an amount ranging from 3% to 7% by weight relative to the weight of the dry extract.

[0096] Advantageously, said extract comprises sugars; preferably, said sugars are present in an amount ranging from 1% to 10% by weight relative to the weight of the dry extract; more preferably, said sugars are present in an amount ranging from 3% to 7% by weight relative to the weight of the dry extract.

[0097] Advantageously, said extract comprises mineral matter; preferably, said mineral matter is present in an amount ranging from 10% to 30% by weight relative to the weight of the dry extract; more preferably, said mineral matter is present in an amount ranging from 15% to 25% by weight relative to the weight of the dry extract.

[0098] According to one preferred embodiment, the said extract comprises:

[0099] - from 20% to 50% by weight of amino acids relative to the total weight of the dry extract, more preferably from 25% to 45% by weight of amino acids relative to the total weight of the dry extract, and

[0100] - from 1% to 10% by weight of lipids relative to the weight of the dry extract, more preferably from 3% to 7% by weight of lipids relative to the weight of the dry extract, and

[0101] - from 1% to 10% by weight of sugars relative to the weight of the dry extract, more preferably from 3% to 7% by weight of sugars relative to the weight of the dry extract, and

[0102] - from 10% to 30% by weight of mineral matter relative to the weight of the dry extract, more preferably from 15% to 25% by weight of mineral matter relative to the weight of the dry extract.

[0103] The extract according to the invention can be used pure or in a composition, in particular a cosmetic composition, more particularly for caring for keratin materials, also comprising a physiologically acceptable medium.

[0104] Advantageously, said extract is present in said composition in an amount ranging from 0.0001% to 20% by weight of dry matter relative to the total weight of the composition, preferably in an amount ranging from 0.001% to 10% by weight of dry matter, more preferably in an amount ranging from 0.01% to 5% by weight of dry matter, and better still in an amount ranging from 0.1% to 1% by weight of dry matter, relative to the total weight of the composition.

[0105] Of course, said composition may comprise an extract of a single type of bacterial strain as indicated above or a mixture of extracts of at least two or more bacterial strains as listed above.

[0106] Said composition is preferably devoid of an extract of any strain other than a strain as listed above.

[0107] The physiologically acceptable medium is generally suited to the nature of the support onto which the composition has to be applied, and also to the appearance under which the composition has to be packaged.

[0108] The physiologically acceptable medium may comprise water and optionally one or more water-miscible solvents.

[0109] According to one preferred embodiment, said composition comprises at least 20% by weight of water, in particular at least 40% by weight of water, relative to the total weight of said composition. Preferentially, said composition has a water content ranging from 20% to 95% by weight, better still from 40% to 90% by weight, relative to the total weight of the composition.

[0110] The water-miscible (at room temperature such as 25°C) organic solvents include lower monoalcohols having from 1 to 5 carbon atoms such as ethanol and isopropanol, glycols having from 2 to 8 carbon atoms such as ethylene glycol, hexylene glycol, propylene glycol, 1,3-butylene glycol, pentylene glycol and dipropylene glycol, and glycerol.

[0111] The water-miscible organic solvent(s) may be present in a concentration of from 0.01% to 30% by weight, preferably from 0.1% to 20% by weight and more preferably from 1% to 15% by weight, relative to the total weight of the composition.

[0112] According to one particular embodiment, the pH of the composition according to the invention is between 4 and 7.5, notably between 4.5 and 7, and in particular between 4.7 and 6.5.

[0113] Said composition may also comprise any water-soluble or water-dispersible compound, such as gelling agents, film-forming polymers, thickeners, surfactants, and mixtures thereof.

[0114] Said composition may also comprise at least one fatty substance, such as one or more oils.

[0115] According to one embodiment, the composition may comprise from 5% to 80% by weight of fatty substance, preferably of oil(s), and preferably from 10% to 60% of fatty substance, preferably of oil(s), by weight relative to the total weight of said composition.

[0116] The term “oil” refers to any fatty substance that is in liquid form at ambient temperature (20-25°C) and at atmospheric pressure. These oils may be of plant, mineral or synthetic origin.

[0117] Oils that can be used in said composition include, for example:

[0118] - hydrocarbon oils of plant origin;

[0119] - synthetic esters and ethers, notably of fatty acids, for instance oils of formulae R1COOR2 and R1OR2 in which R1 represents the residue of a fatty acid comprising from 8 to 29 carbon atoms, and R2 represents a branched or unbranched hydrocarbon chain containing from 3 to 30 carbon atoms;

[0120] - linear or branched hydrocarbons, of mineral or synthetic origin;

[0121] - fatty alcohols having from 8 to 26 carbon atoms;

[0122] - fluoro oils which are partially hydrocarbon-based and / or silicon-based;

[0123] - silicone oils;

[0124] - mixtures thereof.

[0125] In the list of oils mentioned above, a hydrocarbon oil means any oil mainly comprising carbon and hydrogen atoms, and optionally ester, ether, fluoro, carboxylic acid and / or alcohol groups.

[0126] According to a preferred embodiment of the invention, the composition used in the context of the invention is a water-in-oil (W / O) or oil-in-water (O / W) emulsion, preferably an oil-in-water (O / W) emulsion. The proportion of fatty substance in the emulsion may range from 5% to 80% by weight and preferably from 10% to 60% by weight relative to the total weight of the composition.

[0127] The composition may comprise at least one emulsifier. The emulsions generally contain at least one emulsifier chosen from amphoteric, anionic, cationic and non-ionic emulsifiers, used alone or as a mixture, and optionally a coemulsifier. The emulsifiers are appropriately chosen according to the emulsion to be obtained (W / O or O / W).

[0128] The emulsifier and the coemulsifier are generally present in the composition in a proportion ranging from 0.3% to 20% by weight, and preferably from 0.5% to 10% by weight, relative to the total weight of the composition.

[0129] Preferably, said composition may comprise at least one additional ingredient selected from the group consisting of thickeners, fragrances, preservatives, cosmetic active agents other than the extract according to the invention, such as active healing agents other than the extract according to the invention, emollients, fillers and mixtures thereof.

[0130] Such an additional ingredient may represent from 0.0001% to 20%, preferably from 0.01% to 10% and better still from 0.1% to 5% by weight, relative to the total weight of the composition.

[0131] Of course, a person skilled in the art will take care to select these one or more additional ingredients, and / or the amount thereof, such that the advantageous properties of the strains according to the invention are not, or are not substantially, adversely affected by the envisioned addition.

[0132] Said composition may be in any presentation form normally used in the cosmetics field.

[0133] It may notably be in the form of an aqueous or aqueous-alcoholic solution, which may be gelled, a dispersion of the lotion type, which may be a two-phase dispersion, an oil-in-water or water-in-oil emulsion or a multiple emulsion, an aqueous gel, or else a dispersion of oils in an aqueous phase, notably using spherules, it being possible for these spherules to be polymeric particles or, better still, lipid vesicles of ionic and / or nonionic type. It may be of more or less fluid liquid consistency.

[0134] Preferentially, a composition according to the invention differs from compositions having an essentially detergent purpose with regard to the skin, hair and / or mucous membranes, such as soaps, shampoos and shower gels for washing and / or cleansing.

[0135] Said composition is preferentially suitable for topical administration.

[0136] Thus said composition may comprise any constituents usually employed in the envisaged topical application and administration.

[0137] Said composition may advantageously be in the form of an emulsion, particularly obtained by dispersion of an aqueous phase in a fatty phase (W / O) or of a fatty phase in an aqueous phase (O / W), of liquid or semi-liquid consistency of the milk type, or of soft consistency, or even of a multiple emulsion (W / O / W or O / W / O). These compositions are prepared according to the usual known methods.

[0138] More particularly, said composition may be intended for topical application and may preferably be in the form of an emulsion, preferably an oil-in-water emulsion. Preferably, such an emulsion is not intended to be rinsed off after application.

[0139] Said composition is preferably intended to be applied to keratin materials, preferably the skin.

[0140] Said composition according to the invention may be manufactured via any known method generally used in the cosmetics field.

[0141] The ingredients are mixed before shaping, in the order and under conditions readily determined by a person skilled in the art.

[0142] According to one particular mode of the invention, other agents intended to enhance the appearance and / or texture of the skin may also be added to said composition.

[0143] The present invention also relates to a method for non-therapeutic cosmetic treatment of keratin materials, preferably the skin, which comprises topical application to the keratin materials, preferably the skin, of a composition comprising, in a physiologically acceptable medium, an extract according to the invention, for promoting and / or accelerating the healing and / or repair and / or regeneration of keratin materials, preferably the skin.

[0144] The cosmetic uses and processes considered according to the invention are non-therapeutic.

[0145] The cosmetic uses and methods of the invention are preferentially performed by topically administering an extract according to the invention or a composition according to the invention.

[0146] By way of illustration, the cosmetic use or method according to the invention may be implemented by topical application, daily for example, of at least one composition according to the invention, which may be formulated, for example, in the form of a cream, gel, serum, lotion, emulsion, or makeup-removing milk, preferably in the form of an emulsion.

[0147] The application may be repeated, for example, once to twice daily for one or more days and generally over an extended period of at least 4 weeks, or even 4 to 15 weeks, with, where appropriate, one or more periods of stoppage.

[0148] According to one embodiment, the application is daily (once a day) and generally over a prolonged period of at least 4 weeks, or even 4 to 15 weeks, with, where appropriate, one or more periods of stoppage.

[0149] According to one embodiment, the cosmetic treatment method according to the invention may comprise a single application.

[0150] Another subject of the present invention is a method for preparing an extract of at least one bacterium of the speciesStaphylococcus epidermidiscomprising at least one step of culturing at least one bacterium of the speciesStaphylococcus epidermidisin the presence of a culture medium comprising i) at least one monosaccharide, ii) at least one peptone of vegetable origin and / or at least one yeast extract, and optionally iii) at least one microelement.

[0151] The present invention also relates to an extract of at least one bacterium of the speciesStaphylococcus epidermidis, preferably an extract of at least one bacterial strain of the speciesStaphylococcus epidermidisselected from the group consisting of bacteria deposited at the CNCM under the serial numbers I-5688, I-5689, I-5904, I-5691, I-5692, I-5693, I-5694 and I-5695, obtainable, obtained or directly obtained by the abovementioned preparation method.

[0152] The present invention also relates to a biomass extract of at least one bacterial strain of the speciesStaphylococcus epidermidisselected from the group consisting of bacteria deposited at the CNCM under the serial numbers I-5688, I-5689, I-5904, I-5691, I-5692, I-5693, I-5694 and I-5695.

[0153] Advantageously, the steps of the method and also the extract are as defined above.

[0154] Throughout the description, including the claims, the terms “between ... and ...”, and “ranging from ... to ...” should be understood as meaning limits included, unless otherwise specified.

[0155] The examples that follow illustrate the present invention without limiting the scope thereof.

[0156] In the examples, unless otherwise specified, the temperature is room temperature (20°C) and is expressed in degrees Celsius, and the pressure is atmospheric pressure.Examples

[0157] Example 1: Preparation of an extract according to the invention:

[0158] A culture of theStaphylococcus epidermidisstrain CNCM-I 5691 is carried out in its complete culture medium in a 10 effective liter bioreactor in batch or fed batch mode. During this step, the pH is regulated at 6.5 by adding a 1M KOH solution, the temperature is maintained at 32°C and the dissolved oxygen at 40%.

[0159] The composition of the initial culture medium is described in Table 2 below.

[0160] Chemical namesConcentrations by massAutolytic yeast extract5 g / LSoybean papain peptone5 g / LGlucose6.5 g / LKH2PO40.088 g / LCaCl20.050 g / LCuSO4.5H2O60 µg / LMnSO4.1H2O152 µg / LKI20 µg / LZnSO4.7H2O200 µg / LAlCl3.6H2O100 µg / Ldeionized waterqs for 1 L

[0161] As soon as the plateau phase is reached after 20 to 48 hours of culture, the extraction and the separation of the cells are carried out by centrifugation (under laboratory conditions, 10 000 g for 30 min). Then the pellet, also called biomass, containing the cells is recovered, to be subsequently frozen at -20°C and then thawed, allowing the cells to burst and thus a lysate to be obtained. The lysate is then packaged in bags or tubes and finally stabilized by sterilization at 121°C for 30 minutes.

[0162] The lysate obtained at the end of the method as described according to the example contains 5.2% by weight of dry matter, relative to the total weight of the lysate.

[0163] The analysis of the constituents of the dry extract is presented in Table 3 below.

[0164] Compositiong per 100 g of dry extractTotal lipids5.88Total amino acids34.30Total sugars5.29Mineral matter19.01Example 2: Evaluation of keratinocyte migration

[0165] A.Apparatus and method

[0166] The model used was described and published in the publication“3D in vitro model of the re-epithelialization phase in the wound-healing process”,Nathalie Deshayes, Fabienne Bloas, Florian Boissout, Jennifer Lecardonnel, Maryline Paris, published June 11, 2017.

[0167] Adapted from the standard model 3D TSKIN™, the live dermal equivalents (Lattice TSKIN™, Episkin) are weighted by a metal ring. Keratinocytes isolated from biopsies are seeded around the ring, the center remaining empty.

[0168] The ring is removed 10 days after seeding of the keratinocytes. The starting materials to be evaluated for their ability to impact the epidermal regeneration process and epithelialization rate are added to the culture medium. An optical coherence tomography (OCT) analysis method combined with final quantification of histological quality was implemented according to the protocol detailed in the poster “New methodological approach to follow the re-epithelialization phase in the wound-healing process on a 3D full thickness skin model”, Fallou Bénédicte, Pelleter Mylène, Innamorato Florence, Roche Mickael, Bataillon Michel, 25th World Congress of Dermatology, Singapore 2023.

[0169] Optical coherence tomography (OCT) is an optical imaging technique that generates cross-sectional images of tissue structure. OCT achieves this using infrared waves. The use of infrared waves allows for much higher resolution (10 mm) at the expense of lower penetration or imaging depth (2 to 3 mm)(Glinos, G. D., et al. (2017). "Optical coherence tomography for assessment of epithelialization in a human ex vivo wound model"),Wound Repair Regen. 2017 November; 25(6): 1017–1026. This technique enables visualization of the surface appearance and the 2D and 3D structures of the migration model during re-epithelialization and thus makes it possible to follow the closure kinetics for 10 days (from D13 to D20). Through acquisition and segmentation with specific algorithms, the epidermal and dermal thicknesses and also the epidermal profile of the tissue after reconstruction are also determined. Subsequently, the overall morphology of the treated tissues is noted at the end of the treatment by histology on D20.

[0170] Each condition was performed in triplicate.

[0171] B.Results

[0172] The extract ofStaphylococcus epidermidisobtained according to example 1 accelerates epidermal renewal and healing, as shown in Table 3 below.

[0173] Indeed, it has been demonstrated that the extract ofStaphylococcus epidermidisobtained according to example 1 accelerates the migration of keratinocytes, which leads to a faster re-epithelialization; in particular, the extract according to the invention significantly increases (p-value=0.000) the rate of wound closure by +11.4% + / - 2.59 at D14. The effect obtained using the extract according to the invention is close to that obtained with the positive reference of the test (oncostatin M).

[0174] % CLOSURED14D15Starting materials testedEffect(vs untreated)Standard deviationp-valueEffect(vs untreated)Standard deviationp-valueONCOSTATIN Mat 10 ng / mLin a solventPBS + BSA 0.1%14.60%2.670.0009.95%1.70.000Extract ofStaphylococcus epidermidisobtainedaccording to example 1at 250 µg / mLin the culturemedium TSKIN™11.4%2.590.0007.42%1.650.000

Claims

Non-therapeutic cosmetic use of an extract of at least one bacterium of the speciesStaphylococcus epidermidisobtainable by at least one step of culturing at least one bacterium of the speciesStaphylococcus epidermidis, in the presence of a culture medium comprising i) at least one monosaccharide, ii) at least one peptone of vegetable origin and / or at least one yeast extract, and optionally iii) at least one microelement, as an active healing agent, and / or for promoting and / or accelerating the healing and / or repair and / or regeneration of keratin materials, preferably the skin.Non-therapeutic cosmetic use of an extract as claimed in claim 1, characterized in that said culture medium comprises in water:i. at least one monosaccharide present in the culture medium in an amount ranging from 0.5 to 30 g / L, andii. at least one peptone of vegetable origin present in the culture medium in an amount ranging from 0.5 to 15 g / L, and / or at least one yeast extract present in the culture medium in an amount ranging from 0.5 to 15 g / L, andiii. optionally at least one microelement present in the culture medium in an amount ranging from 0.05 to 6 mg / L, andiv. optionally at least one calcium salt present in the culture medium in an amount ranging from 0.01 to 0.1 g / L.Non-therapeutic cosmetic use of an extract as claimed in claim 1 or 2, characterized in that said bacterium of the speciesStaphylococcus epidermidiscultured is a bacterial strain of the speciesStaphylococcus epidermidisselected from the group consisting of bacteria deposited at the CNCM under serial numbers I-5688, I-5689, I-5904, I-5691, I-5692, I-5693, I-5694 and I-5695, preferably a bacterial strain of the speciesStaphylococcus epidermidisselected from the group consisting of bacteria deposited at the CNCM under serial numbers I-5688, I-5691, I-5904, I-5692, I-5693, I-5694 and I-5695, even more preferably a bacterial strain of the speciesStaphylococcus epidermidisdeposited at the CNCM under serial number I-5691.Non-therapeutic cosmetic use of an extract as claimed in any one of the preceding claims, characterized in that it comprises, after said culturing step, a step of separating the bacteria from the culture medium, preferably by centrifugation, filtration, or coagulation, more preferably by centrifugation.Non-therapeutic cosmetic use of an extract as claimed in the preceding claim, characterized in that it comprises, after said separation step, a step of cell lysis, preferably by osmotic shock, thermal shock, ultrasound, or centrifugation, more preferably by thermal shock.Non-therapeutic cosmetic use of an extract as claimed in the preceding claim, characterized in that it comprises, after said cell lysis step, a stabilization step, preferably by autoclaving, ultra-high temperature, high-pressure sterilization, lyophilization, gamma radiation or freezing, preferably by autoclaving.Non-therapeutic cosmetic use of a biomass extract of at least one bacterial strain of the speciesStaphylococcus epidermidisselected from the group consisting of bacteria deposited at the CNCM under serial numbers I-5688, I-5689, I-5904, I-5691, I-5692, I-5693, I-5694 and I-5695 as an active healing agent, and / or for promoting and / or accelerating the healing and / or repair and / or regeneration of keratin materials, preferably the skin.Non-therapeutic cosmetic use of an extract as claimed in any one of the preceding claims, wherein said extract is in the form of a lysate.Method for non-therapeutic cosmetic treatment of keratin materials, preferably the skin, which comprises topical application to the keratin materials, preferably the skin, of a composition comprising, in a physiologically acceptable medium, an extract as defined according to any one of the claims 1 to 8, for promoting and / or accelerating the healing and / or repair and / or regeneration of keratin materials, preferably the skin.Method for non-therapeutic cosmetic treatment of keratin materials as claimed in claim 9, wherein said extract is present in the composition in an amount ranging from 0.0001% to 20% by weight of dry matter relative to the total weight of the composition, preferably in an amount ranging from 0.001% to 10% by weight of dry matter, more preferably in an amount ranging from 0.01% to 5% by weight of dry matter, and better still in an amount ranging from 0.1% to 1% by weight of dry matter relative to the total weight of the composition.