Antibacterial and Anti-inflammatory high-performance hydrogel, and preparation method therefor

Abstract

Disclosed in the present invention are an antibacterial and anti-inflammatory high-performance hydrogel and a preparation method therefor, which belong to the technical field of hydrogels. The hydrogel comprises the following components in parts by weight: 50-60 parts of a high-performance gel and 6-8 parts of an antibacterial sustained-release essential oil, wherein the high-performance gel is obtained by means of crosslinking gelatin, which has been modified with methacrylic acid, and acetylcysteine-loaded hydroxyethyl methacrylate; and the antibacterial sustained-release essential oil is prepared from modified kaolinite and a plant extract. According to the present invention, acetylcysteine is combined with hydroxyethyl methacrylate, which improves the antioxidant capacity of the drug-loaded gel, thereby attenuating inflammatory responses. In addition, acetylcysteine and an antibacterial sustained-release essential oil are used to replace antibiotics, which improves the antibacterial capacity of the gel and reduces the use of antibiotics.

Description

Bacteriostatic anti-inflammatory high-performance hydrogel and preparation method thereof TECHNICAL FIELD

[0001] The present application belongs to the technical field of hydrogel, and particularly relates to a bacteriostatic anti-inflammatory high-performance hydrogel and a preparation method thereof. BACKGROUND

[0002] Existing wound dressings have relatively single functions, most of the wound dressings, such as adhesive plaster, only have hemostatic and isolation functions, and cannot further treat bacterial infection and inflammation at the wound site, some antibiotic gel wound dressings have anti-infection effects, but the extensive use of antibiotics can cause pathogenic bacteria to develop drug resistance; a hydrogel is a high-water-content polymer material forming a three-dimensional network structure through chemical or physical interaction, and has excellent flexibility, good biocompatibility and simple preparation method; acetylcysteine is an amino acid derivative and a strong oxidizing and reducing agent, and is widely used in the treatment of various diseases and symptoms in medicine.

[0003] The existing technology mainly has the following problems: 1. The extensive use of antibiotics causes pathogenic bacteria to develop drug resistance; 2. The currently used wound dressings have single functions. SUMMARY

[0004] In view of the above problems, the present application provides a bacteriostatic anti-inflammatory high-performance hydrogel and a preparation method thereof, in order to solve the problem of single function of the wound dressing, the present application combines acetylcysteine and hydroxyethyl methacrylate to improve the antioxidant capacity of the drug-loaded gel, thereby reducing the inflammatory response, and replaces the antibiotics with acetylcysteine and antibacterial slow-release essential oil to improve the antibacterial capacity of the gel and reduce the use of antibiotics.

[0005] In order to achieve the above purpose, the technical scheme adopted by the present application is as follows: the present application provides a bacteriostatic anti-inflammatory high-performance hydrogel and a preparation method thereof, the bacteriostatic anti-inflammatory high-performance hydrogel comprises the following components by weight: 50-60 parts of high-performance gel, 6-8 parts of antibacterial slow-release essential oil; the high-performance gel is obtained by crosslinking methacrylic acid modified gelatin and acetylcysteine loaded hydroxyethyl methacrylate; the antibacterial slow-release essential oil is prepared from modified kaolinite and plant extract.

[0006] Preferably, the high-performance gel is prepared from the following components by weight: 80-120 parts of gelatin particles, 60-80 parts of glycidyl methacrylate, 90-100 parts of hydroxyethyl methacrylate, and 10-15 parts of acetylcysteine.

[0007] Preferably, the antibacterial slow-release essential oil is prepared from the following components by weight: 10-15 parts of Ascophyllum nodosum, 8-10 parts of Crithmum maritimum, 8-10 parts of Heliotropium europaeum, 5-6 parts of Sea fennel, 15-20 parts of Kaolin, and 12-18 parts of Lecithin.

[0008] Preferably, the preparation method of the high-performance gel comprises the following steps:

[0009] S1, dissolving gelatin particles in deionized water to obtain a gelatin solution, adding glycidyl methacrylate to the gelatin solution, and stirring at 50-60°C for 24h to obtain a GelMA solution;

[0010] S2, dialyzing the GelMA solution obtained in S1 in a dialysis device, dialyzing with deionized water at 40-55°C, and counter-dialyzing with polyethylene glycol-20000 at 40-55°C, and drying to obtain GelMA powder;

[0011] S3, mixing a water solution of photoinitiator 2959 with ethylene glycol at a volume ratio of 1:1 to obtain a mixed solution, adding the GelMA powder obtained in S2 to the mixed solution, and then sequentially adding hydroxyethyl methacrylate and acetylcysteine to the mixed solution, and stirring at 50°C until a complete solution is obtained to obtain a hydrogel preparation solution;

[0012] S4, irradiating the gel preparation solution obtained in S3 under a light source, and then performing ultraviolet sterilization treatment to obtain a high-performance gel.

[0013] Preferably, in S1, the amount of gelatin particles added in deionized water is 35-60mg / mL.

[0014] Preferably, in S2, the dialysis time of deionized water is 40-180h, and the counter-dialysis time of polyethylene glycol-20000 is 40-180h.

[0015] Preferably, in S3, the concentration of the water solution of photoinitiator 2959 is 1-2wt%.

[0016] Preferably, in S3, the amount of GelMA powder added is 0.1-0.2g / mL.

[0017] Preferably, in S4, the wavelength of the light source is 300-400nm, and the irradiation time is 30-100s.

[0018] Preferably, the preparation method of the antibacterial slow-release essential oil comprises the following steps:

[0019] (1) Washing and drying the deep-sea macroalgae, Phyllodoce caerulea, coastal Oenanthe stolonifera and sea fennel at 40 DEG C in an oven, and then crushing and sieving to obtain plant powder, and then extracting the plant powder by using a supercritical CO2 extraction device, under the conditions of an extraction pressure of 30-40 MPa, an extraction temperature of 40-60 DEG C, and an extraction time of 60-100 min, to obtain a composite essential oil;

[0020] (2) dispersing kaolinite in ethanol at a solid-liquid ratio of 1:20-25, adding lecithin, stirring at 8000-10000 rpm at room temperature for 30-40 min, centrifuging, washing, and drying to obtain modified kaolinite;

[0021] (3) dispersing the modified kaolinite obtained in step (3) in distilled water to obtain a kaolinite colloid solution containing 0.5-0.7 wt%, and mixing the kaolinite colloid solution with the composite essential oil obtained in step (1) at a volume ratio of 1:0.2-0.3, and stirring at 6000 rpm for 30 min to obtain an antibacterial slow-release essential oil.

[0022] The application further provides an antibacterial and anti-inflammatory high-performance hydrogel and a preparation method thereof, and specifically comprises the following steps:

[0023] adding the antibacterial slow-release essential oil into the high-performance gel, stirring at 40-50 DEG C in a water bath at 80-100 rpm for 10-15 min, and uniformly mixing to obtain the antibacterial and anti-inflammatory high-performance hydrogel.

[0024] The application has the following beneficial effects: the two networks in the wound dressing can be crosslinked under ultraviolet light to form a gel system; the NAC is loaded in the gel network through a chemical bond -SH to form a disulfide bond, and can be released by responding to oxidative stress in the external environment to reduce inflammatory reactions; the gel network has a broad-spectrum antibacterial effect, and can effectively kill common pathogenic bacteria such as methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli); the use of hydroxyethyl methacrylate makes the gel network have good biocompatibility, can promote blood coagulation at the wound, absorb inflammatory exudate to promote wound healing, and strengthens the mechanical properties of the gel network. BRIEF DESCRIPTION OF DRAWINGS

[0025] Fig. 1 is a high-performance gel technical route of Example 1 of the application;

[0026] Fig. 2 is a photograph of the high-performance gel of Example 1 of the application;

[0027] Fig. 3 is an anti-inflammatory performance test result graph of Example 1-3 and Comparative Examples 2-3 of the application;

[0028] Fig. 4 is a mechanical property test result graph of Example 1 and Comparative Example 1 of the application;

[0029] Figure 5 is a graph of the results of the antibacterial performance test of Example 1 and Comparative Example 2 of the present application.

[0030] The accompanying drawings are included to provide a further understanding of the present application, and are incorporated in and constitute a part of the specification, illustrate embodiments of the present application and, together with the description, serve to explain the present application, but are not intended to limit the present application. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, rather than all the embodiments of the present application. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative work fall within the scope of the present application.

[0032] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as is commonly understood by one of ordinary skill in the art. In addition, any method and material similar or equivalent to those described herein can be used in the present application. The preferred methods and materials described herein are only used as examples, but cannot limit the content of the present application.

[0033] The experimental methods in the following embodiments are all conventional methods unless otherwise specified. The experimental materials used in the following embodiments are all purchased from commercial channels unless otherwise specified.

[0034] Example 1

[0035] A bacteriostatic anti-inflammatory high-performance hydrogel comprises the following components by weight: 60 parts of high-performance gel, 8 parts of antibacterial slow-release essential oil.

[0036] The high-performance gel is prepared from the following components by weight: 120 parts of gelatin particles, 80 parts of glycidyl methacrylate, 100 parts of hydroxyethyl methacrylate, and 15 parts of acetylcysteine.

[0037] The antibacterial slow-release essential oil is prepared from the following components by weight: 15 parts of deep-sea kelp, 10 parts of crinkled horn, 10 parts of coastal parsnip, 6 parts of sea fennel, 20 parts of kaolinite, and 18 parts of lecithin.

[0038] The preparation method of the high-performance gel specifically comprises the following steps:

[0039] S1, dissolve the gelatin particles in deionized water at an addition amount of 60 mg / mL to obtain a gelatin solution, and add glycidyl methacrylate into the gelatin solution, and heat at 60°C for 24 h with stirring at 500 rpm to obtain a GelMA solution;

[0040] S2, the GelMA solution obtained in S1 is placed in a dialysis device for dialysis, deionized water dialysis at 55 DEG C for 180h, polyethylene glycol-20000 counter dialysis at 55 DEG C for 180h, and drying to obtain GelMA powder;

[0041] S3, a 2wt% aqueous solution of photoinitiator 2959 is mixed with ethylene glycol at a volume ratio of 1:1 to obtain a mixed solution, the GelMA powder obtained in S2 is added to the mixed solution at an addition amount of 0.2g / mL, and then hydroxyethyl methacrylate and acetylcysteine are sequentially added to the mixed solution, and the solution is heated and stirred at 50 DEG C until completely dissolved to obtain a hydrogel preparation solution;

[0042] S4, the gel preparation solution obtained in S3 is irradiated under a 400nm light source for 100s, and then ultraviolet sterilization treatment is performed to obtain a high-performance gel.

[0043] The preparation method of the antibacterial slow-release essential oil specifically comprises the following steps:

[0044] (1) The deep-sea kelp, wavy angle, coastal oyster plant, and sea fennel are washed, dried in a 40 DEG C oven, crushed, and sieved through a 60 mesh sieve to obtain plant powder, and the plant powder is extracted by a supercritical CO2 extraction device, the extraction pressure is 40MPa, the extraction temperature is 60 DEG C, and the extraction time is 100min to obtain a composite essential oil;

[0045] (2) The kaolin is dispersed in ethanol at a solid-liquid ratio of 1:25, and lecithin is added, and stirred at 10000rpm at room temperature for 40min, centrifuged, washed, and dried to obtain modified kaolin;

[0046] (3) The modified kaolin obtained in step (3) is dispersed in distilled water to obtain a 0.7wt% kaolin colloid solution, and the kaolin colloid solution is mixed with the composite essential oil obtained in step (1) at a volume ratio of 1:0.3, and stirred at 6000rpm for 30min to obtain an antibacterial slow-release essential oil.

[0047] The application also provides an antibacterial and anti-inflammatory high-performance hydrogel and a preparation method thereof, which specifically comprises the following steps:

[0048] The antibacterial slow-release essential oil is added to the high-performance gel, and stirred at 50 DEG C in a water bath at 100rpm for 15min, and uniformly mixed to obtain an antibacterial and anti-inflammatory high-performance hydrogel.

[0049] Example 2

[0050] An antibacterial and anti-inflammatory high-performance hydrogel comprises the following components by weight: 50 parts of high-performance gel, and 6-8 parts of antibacterial slow-release essential oil.

[0051] The high-performance gel is prepared from the following components in parts by weight: gelatin particles 80 parts, glycidyl methacrylate 60 parts, hydroxyethyl methacrylate 90 parts, acetylcysteine 10 parts.

[0052] The antibacterial slow-release essential oil is prepared from the following components in parts by weight: deep-sea kelp 10 parts, crinkled angle 8 parts, coastal oenanthe 8 parts, sea anise 5 parts, kaolinite 15 parts, lecithin 12 parts.

[0053] The preparation method of the high-performance gel specifically comprises the following steps:

[0054] S1, dissolve gelatin particles in deionized water to obtain a gelatin solution, add glycidyl methacrylate to the gelatin solution, and heat at 50°C with stirring at 500 rpm for 24 hours to obtain a GelMA solution;

[0055] S2, dialyze the GelMA solution obtained in S1 in a dialysis device, dialyze against deionized water at 40°C for 40 hours, and against polyethylene glycol-20000 at 40°C for 40 hours, and dry to obtain GelMA powder;

[0056] S3, blend a 1wt% aqueous solution of photoinitiator 2959 with ethylene glycol at a volume ratio of 1:1 to obtain a mixed solution, add the GelMA powder obtained in S2 to the mixed solution at an addition amount of 0.1g / mL, and then sequentially add hydroxyethyl methacrylate and acetylcysteine to the mixed solution, and heat and stir at 50°C until a complete solution is obtained to obtain a hydrogel preparation solution;

[0057] S4, irradiate the gel preparation solution obtained in S3 under a 300nm light source for 30s, and then perform ultraviolet sterilization to obtain a high-performance gel.

[0058] The preparation method of the antibacterial slow-release essential oil specifically comprises the following steps:

[0059] (1) Wash the deep-sea kelp, crinkled angle, coastal oenanthe, and sea anise, dry them in a 40°C oven, and then crush and pass through a 60-mesh sieve to obtain plant powder, and extract the plant powder using a supercritical CO2 extraction device, with an extraction pressure of 30MPa, an extraction temperature of 40°C, and an extraction time of 60min to obtain a composite essential oil;

[0060] (2) Disperse kaolinite in ethanol at a solid-liquid ratio of 1:20, add lecithin, stir at room temperature at 8000rpm for 30min, centrifuge, wash, and dry to obtain modified kaolinite;

[0061] (3) the modified kaolinite obtained in step (3) is dispersed in distilled water to obtain a kaolinite colloid solution containing 0.5 wt%, the kaolinite colloid solution is mixed with the composite essential oil obtained in step (1) at a volume ratio of 1:0.2, stirring is performed at 6000 rpm for 30 min to obtain the antibacterial slow-release essential oil.

[0062] The application further provides an antibacterial and anti-inflammatory high-performance hydrogel and a preparation method thereof, and specifically comprises the following steps:

[0063] The antibacterial slow-release essential oil is added into the high-performance gel, stirring is performed at 40 DEG C in a water bath at 80 rpm for 10 min, and mixing is uniformly performed to obtain the antibacterial and anti-inflammatory high-performance hydrogel.

[0064] Example 3

[0065] An antibacterial and anti-inflammatory high-performance hydrogel comprises the following components in parts by weight: high-performance gel 55 parts, antibacterial slow-release essential oil 7 parts.

[0066] The high-performance gel is prepared from the following components in parts by weight: gelatin particles 100 parts, glycidyl methacrylate 70 parts, hydroxyethyl methacrylate 95 parts, acetylcysteine 12 parts.

[0067] The antibacterial slow-release essential oil is prepared from the following components in parts by weight: deep-sea macroalgae 12 parts, undulate angle 9 parts, coastal oenanthe 9 parts, sea anise 5.5 parts, kaolinite 18 parts, lecithin 15 parts.

[0068] The preparation method of the high-performance gel specifically comprises the following steps:

[0069] S1, gelatin particles are dissolved in deionized water at an addition amount of 40 mg / mL to obtain a gelatin solution, glycidyl methacrylate is added into the gelatin solution, 55 DEG C heat preservation is performed at 500 rpm for 24 h to obtain a GelMA solution;

[0070] S2, the GelMA solution obtained in S1 is placed in a dialysis device for dialysis, dialysis is performed in deionized water at 50 DEG C for 60 h, reverse dialysis is performed in polyethylene glycol-20000 at 50 DEG C for 60 h, and drying is performed to obtain GelMA powder;

[0071] S3, 1.5 wt% aqueous solution of a photoinitiator 2959 is mixed with ethylene glycol at a volume ratio of 1:1 to obtain a mixed solution, the GelMA powder obtained in S2 is added into the mixed solution at an addition amount of 0.15 g / mL, and then hydroxyethyl methacrylate and acetylcysteine are sequentially added into the mixed solution, 50 DEG C heating and stirring are performed until a complete solution is obtained to obtain a hydrogel preparation solution;

[0072] S4, the gel preparation solution obtained in S3 is irradiated under a 350 nm light source for 60 s, and then ultraviolet sterilization treatment is performed to obtain the high-performance gel.

[0073] The preparation method of the antibacterial slow-release essential oil specifically comprises the following steps:

[0074] (1) Wash the giant kelp, ruffled angle, coastal oyster plant, and sea fennel, dry them in a 40℃ oven, and then crush them to obtain plant powder, and then extract the plant powder by using a supercritical CO2 extraction device, wherein the extraction pressure is 35MPa, the extraction temperature is 50℃, and the extraction time is 80min, to obtain a composite essential oil;

[0075] (2) Disperse kaolinite in ethanol at a solid-liquid ratio of 1:22, add lecithin, stir at 9000rpm for 30-40min at room temperature, centrifuge, wash, and dry to obtain modified kaolinite;

[0076] (3) Disperse the modified kaolinite obtained in step (3) in distilled water to obtain a kaolinite colloid solution containing 0.5-0.7wt%, and then mix the kaolinite colloid solution with the composite essential oil obtained in step (1) at a volume ratio of 1:0.2-0.3, and stir at 6000rpm for 30min to obtain the antibacterial slow-release essential oil.

[0077] The application further provides a bacteriostatic and anti-inflammatory high-performance hydrogel and a preparation method thereof, which specifically comprises the following steps:

[0078] Add the antibacterial slow-release essential oil to the high-performance gel, and then stir at 45℃ in a water bath at 90rpm for 12min, so as to obtain the bacteriostatic and anti-inflammatory high-performance hydrogel.

[0079] Comparative Example 1

[0080] The present comparative example provides a dressing, which is different from Example 1 only in that the component does not contain hydroxyethyl methacrylate (HEMA), and the other components and component contents are the same as those of Example 1.

[0081] Comparative Example 2

[0082] The present comparative example provides a dressing, which is different from Example 1 only in that the component does not contain acetylcysteine (NAC), and the other components and component contents are the same as those of Example 1.

[0083] Experimental Example

[0084] 1. Anti-inflammatory performance test

[0085] The 8-week-old SD rats were selected to prepare the diabetic rats by intraperitoneal injection of streptozotocin, and 10 normal rats were used as blank group, and 40 diabetic rats were used as experimental group, and the rats in the experimental group were randomly divided into four groups; the rats were anesthetized, the back hair was shaved, and a full-thickness skin wound with a diameter of 8 mm was created on the back by using a punch, and the wound rats were prepared; the rats in the experimental group were coated with Examples 1-3 and Comparative Example 2, and the rats in the blank group were not treated; the serum tumor necrosis factor-α (TNF-α) level of the rats was determined by chemiluminescence method at 1d and 7d, respectively.

[0086] 2. Mechanical property test

[0087] The electronic universal testing machine was used to test the mechanical properties of Example 1 (GelMA-HEMA gel) and Comparative Example 1 (GelMA gel), and the hydrogel sample for compression test was in cylindrical shape, and the compression rate was 2mm / min, and the compression stress-strain curve was drawn.

[0088] 3. Antimicrobial property test

[0089] The antibacterial experiment used E. coli cells, and the hydrogel leachate of Example 1 (GelMA-HEMA gel) and Comparative Example 2 (GelMA gel) was sterilized, and the suspension of E. coli was inoculated in agar culture medium, 1mL of leachate of Examples 1-2 was added as experimental group, and 1mL of physiological saline was added as control group, and the culture was incubated at 37℃ for 24h, and then 1mL of culture was cultured in agar culture medium at 37℃ for 30h, and the optical photograph was used to evaluate the antibacterial effect of the gel sample.

[0090] FIG. 1 is a technical route diagram of the high-performance gel of Example 1 of the present application; as shown in the figure, the preparation path of the high-performance gel is clear, the preparation process is simple, and it is easy to produce.

[0091] FIG. 2 is a photograph of the high-performance gel of Example 1 of the present application; as shown in the figure, the gel obtained after light crosslinking is in solid transparent state.

[0092] FIG. 3 is a graph of the anti-inflammatory performance test results of Examples 1-3 and Comparative Example 2 of the present application; as shown in the figure, the 1d serum tumor necrosis factor-α (TNF-α) levels of the blank group, Examples 1-3 and Comparative Example 2 were 2.36ng / mL, 4.02ng / mL, 4.04ng / mL, 4.03ng / mL, 4.06ng / mL, respectively, and the 7d serum tumor necrosis factor-α (TNF-α) levels were 2.52ng / mL, 2.32ng / mL, 2.35ng / mL, 2.29ng / mL, 3.25ng / mL, respectively; as shown in the figure, the serum tumor necrosis factor-α concentration of Examples 1-3 at 7d was significantly decreased compared with that at 1d, the blank group was slightly increased, and the decrease of Comparative Example 2 was less than that of Examples 1-3, which indicated that the use of acetylcysteine improved the anti-inflammatory effect.

[0093] Figure 4 is a graph of the mechanical property test results of Example 1 and Comparative Example 1 of the present application; as shown, the compressive strength of Example 1 is significantly better than that of Comparative Example 1, the addition of hydroxyethyl methacrylate increases the crosslinking density of the material, and improves the mechanical properties of the gel material.

[0094] Figure 5 is a graph of the antibacterial property test results of Example 1 and Comparative Example 2 of the present application; as shown, the antibacterial effect of Example 1 is significantly better than that of Comparative Example 2, the addition of acetylcysteine improves the antibacterial properties of the gel material.

[0095] Although embodiments of the present application have been shown and described, it is to be understood that various modifications, substitutions, replacements and changes can be made to these embodiments without departing from the principles and spirit of the present application, which will be appreciated by those skilled in the art.

[0096] The above description of the present application and its embodiments is not restrictive, and the embodiments shown in the drawings are only one of the embodiments of the present application, and the actual application is not limited thereto. In summary, if a person skilled in the art is inspired by it, without departing from the purpose of the present application, without creative design, similar ways and embodiments of the technical solution should belong to the protection scope of the present application.

Claims

1. A bacteriostatic anti-inflammatory high performance hydrogel, characterized in that: The bacteriostatic anti-inflammatory high-performance hydrogel comprises the following components in parts by weight: high-performance gel 50-60 parts, antibacterial slow-release essential oil 6-8 parts; the high-performance gel is obtained by crosslinking of methacrylic acid modified gelatin and acetylcysteine loaded hydroxyethyl methacrylate; the antibacterial slow-release essential oil is prepared from modified kaolinite and plant extracts; The high-performance gel is prepared from the following components in parts by weight: gelatin particles 80-120 parts, glycidyl methacrylate 60-80 parts, hydroxyethyl methacrylate 90-100 parts, acetylcysteine 10-15 parts; The antibacterial slow-release essential oil is prepared from the following components in parts by weight: deep-sea kelp 10-15 parts, undulate angle 8-10 parts, coastal oyster plant 8-10 parts, sea anise 5-6 parts, kaolinite 15-20 parts, lecithin 12-18 parts.

2. A process for the preparation of the bacteriostatic anti-inflammatory high- performance hydrogel according to claim 1, characterized by the fact that: Specifically comprising the following steps: The antibacterial slow-release essential oil is added to the high-performance gel, and stirring is carried out at 40-50°C in a water bath at 80-100 rpm for 10-15 min, and then uniform mixing is carried out to obtain the bacteriostatic anti-inflammatory high-performance hydrogel.

3. The method for preparing bacteriostatic anti-inflammatory high-performance hydrogel according to claim 2, characterized in that: The preparation method of the high-performance gel specifically comprises the following steps: S1, gelatin particles are dissolved in deionized water to obtain a gelatin solution, glycidyl methacrylate is added to the gelatin solution, and stirring is carried out at 50-60°C at 500 rpm for 24 h to obtain a GelMA solution; S2, the GelMA solution obtained in S1 is placed in a dialysis device for dialysis, and dialysis is carried out in deionized water at 40-55°C, and counter-dialysis is carried out in polyethylene glycol-20000 at 40-55°C, and then drying is carried out to obtain GelMA powder; S3, a mixed solution is obtained by blending a water solution of photoinitiator 2959 and ethylene glycol at a volume ratio of 1:1, the GelMA powder obtained in S2 is added to the mixed solution, and then hydroxyethyl methacrylate and acetylcysteine are sequentially added to the mixed solution, and then complete solution is obtained by stirring at 50°C to obtain a hydrogel preparation solution; S4, the gel preparation solution obtained in S3 is irradiated under a light source, and then ultraviolet sterilization treatment is carried out to obtain a high-performance gel.

4. The method of preparation of bacteriostatic anti-inflammatory high performance hydrogel according to claim 3, characterized in that: The preparation method of the antibacterial slow-release essential oil specifically comprises the following steps: (1) the deep-sea kelp, undulate angle, coastal oyster plant and sea anise are washed, dried in a 40°C oven, crushed, and sieved through a 60-mesh sieve to obtain plant powder, and then the plant powder is extracted by a supercritical CO2 extraction device, the extraction pressure is 30-40 MPa, the extraction temperature is 40-60°C, and the extraction time is 60-100 min to obtain a composite essential oil; (2) kaolinite is dispersed in ethanol at a solid-liquid ratio of 1:20-25, lecithin is added, stirring is carried out at 8000-10000 rpm at room temperature for 30-40 min, centrifugation is carried out, washing is carried out, and then drying is carried out to obtain modified kaolinite; (3) the modified kaolinite obtained in step (3) is dispersed in distilled water to obtain a kaolinite colloid solution containing 0.5-0.7wt%, and then the kaolinite colloid solution and the composite essential oil obtained in step (1) are mixed at a volume ratio of 1:0.2-0.3, and stirring is carried out at 6000 rpm for 30 min to obtain an antibacterial slow-release essential oil.

5. The method of preparation of bacteriostatic anti-inflammatory high performance hydrogel according to claim 4, characterized in that: In S1, the added amount of the gelatin particles in deionized water is 35-60 mg / mL.

6. The method of preparation of bacteriostatic anti-inflammatory high performance hydrogel according to claim 5, characterized in that: In S2, the dialysis time of the deionized water is 40-180 h; the counter-dialysis time of the polyethylene glycol-20000 is 40-180 h.

7. The method of preparation of bacteriostatic anti-inflammatory high performance hydrogel according to claim 6, characterized in that: In S3, the concentration of the aqueous solution of the photoinitiator I-2959 is 1-2 wt%; the added amount of the GelMA powder is 0.1-0.2 g / mL.

8. The method of preparation of bacteriostatic anti-inflammatory high performance hydrogel according to claim 7, characterized in that: In S4, the wavelength of the light source is 300-400 nm, and the irradiation time is 30-100 s.

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