Compound essential oil for stimulating hair growth and preventing hair loss, and preparation method therefor

Abstract

A compound essential oil for stimulating hair growth and preventing hair loss, comprising the following raw materials: 1-5 parts by weight of cedarwood essential oil, 0.5-3 parts by weight of peppermint essential oil, 1-5 parts by weight of rosemary essential oil, 0.5-3 parts by weight of lavender essential oil, 0.1-1 part by weight of sandalwood essential oil, 1-5 parts by weight of thuja essential oil, 0.01-1 part by weight of petitgrain bigarade essential oil, 1-5 parts by weight of ginger essential oil, 0.01-1 part by weight of Taiwan hinoki essential oil, 0.01-1 part by weight of lemon essential oil, 5-15 parts by weight of argan oil, 40-65 parts by weight of sesame oil, 10-30 parts by weight of jojoba oil, and 1-10 parts by weight of Nigella sativa seed oil. The compound essential oil has excellent effects on promoting hair growth and preventing hair loss.

Description

A compound essential oil for hair growth and hair loss prevention and its preparation method Technical Field

[0001] This invention relates to the field of pharmaceutical technology, specifically to a compound essential oil for hair growth and hair loss prevention, and its preparation method. Background Technology

[0002] Hair loss refers to the phenomenon of hair falling out, including both physiological and pathological hair loss. Hair growth has a cyclical nature, consisting of the anagen (growth) phase, catagen (transitional) phase, and telogen (resting) phase. During the catagen and telogen phases, a small amount of hair loss is normal, maintaining a dynamic balance with the anagen phase. Pathological hair loss is a common dermatological condition and a prevalent global phenomenon increasingly affecting younger people. Based on its cause, it can be classified into several types, including androgenetic alopecia, neurogenic alopecia, endocrine alopecia, irritant alopecia, and infectious alopecia. Hair loss severely impacts a patient's appearance, indicating a sub-healthy state and causing serious adverse effects on their physical and mental well-being. Therefore, solutions to hair loss and gray hair problems have attracted widespread attention.

[0003] Surgical treatments, including hair transplantation and scalp expansion, are modern techniques for treating hair loss, but they are costly, complex, and prone to scarring. Currently, the main medications used clinically to treat hair loss are the chemical drugs minoxidil and finasteride. Minoxidil has a relatively high safety profile, but its efficacy remains controversial, and it has been reported to have adverse reactions such as dose dependence, mild dermatitis, allergies, and irritation; finasteride, on the other hand, has side effects such as sexual dysfunction and psychological problems. Therefore, there is an urgent need to develop new treatment products to alleviate hair loss problems.

[0004] Plant essential oils are small-molecule aromatic substances extracted from natural plants through methods such as distillation and cold pressing. They are highly volatile, have small molecules, are easily absorbed by the human body, and have few side effects. The aroma of essential oils can improve the patient's psychological state, and their rich chemical components can also synergistically improve hair loss problems affected by various factors.

[0005] Summary of the Invention

[0006] To overcome the deficiencies or shortcomings of existing technologies, the primary objective of this invention is to provide a compound essential oil for hair growth and hair loss prevention. A secondary objective of this invention is to provide a method for preparing the compound essential oil for hair growth and hair loss prevention.

[0007] To achieve the above objectives, the present invention adopts the following technical solution:

[0008] As a first aspect of the present invention, a compound essential oil for hair growth and hair loss prevention is provided, characterized in that it comprises the following raw materials: 1-5 parts by weight of cedarwood essential oil, 0.5-3 parts by weight of peppermint essential oil, 1-5 parts by weight of rosemary essential oil, 0.5-3 parts by weight of lavender essential oil, 0.1-1 parts by weight of sandalwood essential oil, 1-5 parts by weight of cypress essential oil, 0.01-1 parts by weight of bitter orange leaf essential oil, 1-5 parts by weight of ginger essential oil, 0.01-1 parts by weight of yellow cypress essential oil, 0.01-1 parts by weight of lemon essential oil, 5-15 parts by weight of argan oil, 40-65 parts by weight of sesame oil, 10-30 parts by weight of jojoba oil, and 1-10 parts by weight of black cumin seed oil.

[0009] Furthermore, the raw materials are as follows: 1 part by weight of cedarwood essential oil, 0.5 part by weight of peppermint essential oil, 1 part by weight of rosemary essential oil, 0.5 part by weight of lavender essential oil, 0.1 part by weight of sandalwood essential oil, 1 part by weight of cypress essential oil, 0.01 part by weight of bitter orange leaf essential oil, 1 part by weight of ginger essential oil, 0.01 part by weight of yellow cypress essential oil, 0.01 part by weight of lemon essential oil, 5 parts by weight of argan oil, 40 parts by weight of sesame oil, 10 parts by weight of jojoba oil, and 1 part by weight of black cumin seed oil.

[0010] Furthermore, the ingredients are as follows: 5 parts by weight of cedarwood essential oil, 3 parts by weight of peppermint essential oil, 5 parts by weight of rosemary essential oil, 3 parts by weight of lavender essential oil, 1 part by weight of sandalwood essential oil, 5 parts by weight of cypress essential oil, 1 part by weight of bitter orange leaf essential oil, 5 parts by weight of ginger essential oil, 1 part by weight of yellow cypress essential oil, 1 part by weight of lemon essential oil, 15 parts by weight of argan oil, 65 parts by weight of sesame oil, 30 parts by weight of jojoba oil, and 10 parts by weight of black cumin seed oil.

[0011] According to the information, the effects of each ingredient essential oil are as follows:

[0012] Cedar: Cedrus atlantica

[0013] Peppermint: Mentha × piperita Linn.

[0014] Rosemary: Rosmarinus officinalis Linn.

[0015] Lavender: Lavandula angustifolia Mill.

[0016] Sandalwood: Santalum spicatum

[0017] Chinese arborvitae: Thuja occidentalis Linn.

[0018] Bitter orange leaf: Citrus aurantium Linn.

[0019] Ginger: Zingiber officinale Rose.

[0020] Yellow cypress: Chamaecyparis obtusa var.formosana(Hayata)Rehd.

[0021] Lemon: Citrus limon(L.) Osbeck

[0022] Argania spinosa Linn. (Moroccan nut)

[0023] Sesame: Sesamum indicum Linn.

[0024] Jojoba: Simmondsia chinensis(Link)CKSchneid.

[0025] Black cumin: Nigella Sativa Linn.

[0026] Cedarwood essential oil:

[0027] Efficacy: It improves bronchitis, cough, runny nose, and other problems, and is effective against cold sores, chickenpox, and shingles. It also soothes nervous tension and anxiety, and has mosquito-repellent properties.

[0028] Hair growth and hair loss prevention: Cedarwood essential oil is an excellent hair conditioning oil that can effectively combat dandruff and hair loss.

[0029] Peppermint essential oil:

[0030] Efficacy: Soothes the throat, eliminates bad breath, relieves physical and mental discomfort, and treats colds, dry coughs, asthma, etc.

[0031] Hair growth and hair loss prevention: Although peppermint essential oil is not primarily used for hair growth and hair loss prevention, its cooling properties may help soothe the scalp and indirectly promote scalp health.

[0032] Rosemary essential oil:

[0033] Benefits: Improves memory and concentration, relieves stress and anxiety, strengthens the immune system, promotes digestion, and improves skin problems.

[0034] Hair growth and hair loss prevention: Rosemary essential oil has antibacterial and anti-inflammatory properties, which may help improve the scalp environment, thereby promoting hair growth and preventing hair loss.

[0035] Lavender essential oil:

[0036] Efficacy: antibacterial, calming, skin care, improves muscle soreness, and enhances immunity.

[0037] Hair growth and hair loss prevention: Lavender essential oil has a cleansing and skin care effect, can promote cell metabolism, and has a certain promoting effect on the regeneration and recovery of damaged tissues. It may help scalp health, thereby promoting hair growth and preventing hair loss.

[0038] Sandalwood essential oil:

[0039] Efficacy: Regulates emotions, relieves fatigue, enhances immunity, and has the effects of regulating qi and relieving depression, warming the spleen and stopping vomiting, and dispelling foulness and detoxifying.

[0040] Hair growth and hair loss prevention: Sandalwood essential oil is believed in Traditional Chinese Medicine to have the effect of regulating qi and relieving depression, which may be beneficial to scalp health, but its direct effect on hair growth and hair loss prevention is unclear.

[0041] Chinese arborvitae essential oil:

[0042] Efficacy: Antibacterial and anti-inflammatory, astringent, diuretic, softening, expectorant, antifungal, and tonic.

[0043] Hair growth and hair loss prevention: Thuja orientalis essential oil has antibacterial and anti-inflammatory effects, which may help improve the scalp environment and reduce hair loss, but its direct effect on hair growth is not clear.

[0044] Bitter orange leaf essential oil:

[0045] Benefits: Relieves fatigue, improves sleep quality, has anti-depressant effects, promotes digestion, and alleviates respiratory discomfort.

[0046] Hair growth and hair loss prevention: Bitter orange leaf essential oil mainly focuses on relieving fatigue and improving sleep quality. Although good sleep may indirectly promote hair health, its direct effects on hair growth and hair loss prevention are not significant.

[0047] Ginger essential oil:

[0048] Hair growth and hair loss prevention effects: Ginger essential oil is mainly extracted from the fresh rhizomes of ginger, a plant in the ginger family. It contains gingerol, gingerol, and other components that can replenish the nutrients needed by hair and promote hair growth. Ginger essential oil can improve dry, split, and brittle hair, making hair darker and shinier, thus achieving the effect of promoting hair growth and preventing hair loss.

[0049] Yellow cypress essential oil:

[0050] Hair growth and hair loss prevention benefits: It is often used as a natural oil to moisturize and protect the skin and hair, and may help improve the shine and texture of the hair.

[0051] Lemon essential oil:

[0052] Hair growth and hair loss prevention effects: Lemon essential oil is mainly used to improve oily hair, making hair fresh and flowing.

[0053] Argan oil:

[0054] Hair growth and hair loss prevention benefits: Argan oil can nourish the scalp, strengthen hair roots, prevent hair breakage and split ends, and has the effect of preventing hair loss.

[0055] Sesame oil:

[0056] Hair growth and hair loss prevention benefits: Sesame seed oil is rich in vitamin E and essential fatty acids, which help protect hair from free radical damage and maintain hair health. The linoleic acid and other unsaturated fatty acids in sesame seed oil provide essential nutrients for hair, making it smooth and shiny. The zinc, iron, and other trace elements in sesame seed oil can stimulate hair follicle cell activity and accelerate the hair growth cycle.

[0057] Jojoba oil:

[0058] Hair growth and hair loss prevention benefits: Jojoba oil is rich in vitamins and trace elements such as calcium, which can provide appropriate nutrition to the hair and protect it from external damage. Jojoba oil has a certain repairing effect on damaged keratinocytes of the scalp, and for those with dry hair, it can nourish the hair and make it easier to comb.

[0059] Black cumin seed oil:

[0060] Hair growth and hair loss prevention effects: Black cumin seed oil contains a large amount of unsaturated fatty acids, which can promote blood circulation and benefit the growth and development of hair follicles, thus improving hair loss to a certain extent. Its deep moisturizing properties also help improve the pigment nutrition of hair follicle matrix cells in the hair bulb, gradually restoring the function of melanin synthesis, thereby playing a role in darkening hair and inhibiting gray hair.

[0061] As a second aspect of the present invention, a method for preparing the compound essential oil for hair growth and hair loss prevention as described in any of the above claims is provided, comprising the following steps:

[0062] S1. Take cedarwood, peppermint, rosemary, lavender, sandalwood, arborvitae, bitter orange leaf, ginger and yellow cypress, and extract them by steam distillation.

[0063] S2. Extract lemon, argan oil, sesame, jojoba seeds, and black cumin seeds using a cold pressing method;

[0064] S3. Mix the essential oils obtained in the above steps according to the stated weight proportions to obtain a compound essential oil.

[0065] The essential oils are derived from the leaves and branches of Atlantic cedar and rosemary, the plant of peppermint, the rhizome of ginger, the flower of lavender, the leaves of cypress and bitter orange, the wood of sandalwood and yellow cypress, the peel of lemon, the kernel of argan oil, and the seeds of sesame, jojoba, and black cumin.

[0066] Furthermore, the preparation method includes the following steps:

[0067] S1. Take cedarwood, peppermint, rosemary, lavender, sandalwood, arborvitae, bitter orange leaf, ginger, and cypress wood. Wash them clean and crush them separately to obtain corresponding plant solid fragments. Use steam distillation to extract each plant solid fragment separately. Filter the extracted products and collect the filtrate to obtain cedarwood essential oil, peppermint essential oil, rosemary essential oil, lavender essential oil, sandalwood essential oil, arborvitae essential oil, bitter orange leaf essential oil, ginger essential oil, and cypress wood essential oil, respectively.

[0068] S2. Take lemon, argan oil, sesame, jojoba seeds and black cumin seeds, wash them clean and crush them, and then extract them by cold pressing to obtain lemon essential oil, argan oil, sesame oil, jojoba oil and black cumin seed oil respectively.

[0069] S3. Mix the cedarwood essential oil, peppermint essential oil, rosemary essential oil, lavender essential oil, sandalwood essential oil, cypress essential oil, bitter orange leaf essential oil, ginger essential oil, yellow cypress essential oil, lemon essential oil, argan oil, sesame oil, jojoba oil, and black cumin seed oil obtained in the above steps according to the stated weight proportions to obtain a compound essential oil.

[0070] When extracting each plant essential oil using steam distillation in S1, the mass ratio of water to each pulverized plant solid fragment is 4:1-6:1.

[0071] In step S1, when steam distillation is used to extract the essential oils from each plant, the mass ratio of water to the pulverized plant solid fragments is 5:1.

[0072] In S2, the specific process of cold pressing to extract plant essential oils is as follows: physical pressing by machine → separation of oil by centrifuge → filtration of the extracted product and collection of the second filtrate, the obtained second filtrate being the corresponding essential oil.

[0073] In step S2, when extracting plant essential oils using the cold pressing method, the process must be carried out at 5-10℃. Attached Figure Description

[0074] Figure 1. Gender distribution of all participants

[0075] Figure 2. Hair distribution of all participants

[0076] Figure 3. Duration of hair loss in all participants.

[0077] Figure 4. Hair loss status of family members of all participants.

[0078] Figure 5. Effects on the test subjects after use in Example 1.

[0079] Figure 6. Effects on the test subjects after use in Example 2.

[0080] Figure 7. Effects on the test subjects after use in Example 3.

[0081] Figure 8. Effects on the test subjects after use in Example 4.

[0082] Figure 9. Examples of using hair growth and anti-hair loss compound essential oil.

[0083] Figure 10. Establishment of the mouse alopecia model

[0084] Figure 11. Mouse alopecia model after treatment for 1 week following modeling.

[0085] Figure 12. Mouse alopecia model after 2 weeks of treatment following modeling.

[0086] Figure 13 Hair Length

[0087] Figure 14 Long Hair Rate

[0088] Figure 15 AST Level

[0089] Figure 16 ALT Level

[0090] Figure 17 ALB Level

[0091] Figure 18 ALP Level

[0092] Figure 19 BUN Levels

[0093] Figure 20 UA Level

[0094] Figure 21 CK Level

[0095] Figure 22 GLU Levels

[0096] Figure 23 TG Level

[0097] Figure 24 LDH Level Detailed Implementation

[0098] The present invention will be further explained and illustrated below with reference to specific embodiments. These embodiments are for illustrative purposes only and are not intended to limit the scope of the invention. Experimental methods in the following embodiments, unless otherwise specified, are generally performed under conventional conditions.

[0099] Example 1

[0100] Source of materials:

[0101] The cedarwood, peppermint, rosemary, lavender, sandalwood, arborvitae, bitter orange leaves, ginger, yellow cypress, lemon, argan oil, sesame, jojoba seeds, and black cumin seeds used in the following examples all comply with the relevant provisions of the Chinese Pharmacopoeia (2015 edition), Part I, under the respective medicinal materials section. Before feeding, each medicinal material was identified to ensure that the actual product matched the name and that the quality met the standards. Specifically, the essential oils were extracted from the branches and leaves of Atlantic cedarwood and rosemary, the plants of peppermint, the rhizomes of ginger, the flowers of lavender, the leaves of arborvitae and bitter orange, the wood of sandalwood and yellow cypress, the peel of lemon, the kernels of argan oil, and the seeds of sesame, jojoba, and black cumin.

[0102] S1. Take 100g each of cedarwood, peppermint, rosemary, lavender, sandalwood, arborvitae, petitgrain, ginger, and cypress wood, wash and grind them. Then, add them separately to a distillation apparatus, add 450ml of water, and perform steam distillation extraction at 30℃. After 300min, remove the heat, collect the distillation products, and obtain cedarwood essential oil, peppermint essential oil, rosemary essential oil, lavender essential oil, sandalwood essential oil, arborvitae essential oil, petitgrain essential oil, ginger essential oil, and cypress wood essential oil.

[0103] S2. Take 100g each of lemon, argan oil, sesame seeds, jojoba seeds, and black cumin seeds, wash them thoroughly, and extract them separately using a cold pressing method. When pressing using a physical machine, maintain the temperature at 8℃ and isolate them from oxygen and light. Then, use a centrifuge to separate the oils, filter the extracted products, and collect the second filtrate to obtain lemon essential oil, argan oil, sesame oil, jojoba oil, and black cumin seed oil, respectively.

[0104] S3. The products obtained in steps S1 and S2 are combined according to the following proportions: 5 parts by weight of cedarwood essential oil, 3 parts by weight of peppermint essential oil, 5 parts by weight of rosemary essential oil, 3 parts by weight of lavender essential oil, 1 part by weight of sandalwood essential oil, 5 parts by weight of cypress essential oil, 1 part by weight of bitter orange leaf essential oil, 5 parts by weight of ginger essential oil, 1 part by weight of yellow cypress essential oil, 1 part by weight of lemon essential oil, 15 parts by weight of argan oil, 65 parts by weight of sesame oil, 30 parts by weight of jojoba oil, and 10 parts by weight of black cumin seed oil.

[0105] Mix thoroughly to obtain the compound essential oil of Example 1, and place it in a brown bottle for later use.

[0106] Example 2

[0107] S1. Take 100g each of cedarwood, peppermint, rosemary, lavender, sandalwood, arborvitae, petitgrain, ginger, and cypress wood, wash and grind them. Then, add them separately to a distillation apparatus, add 450ml of water, and perform steam distillation extraction at 30℃. After 250min, remove the heat, collect the distillation products, and obtain cedarwood essential oil, peppermint essential oil, rosemary essential oil, lavender essential oil, sandalwood essential oil, arborvitae essential oil, petitgrain essential oil, ginger essential oil, and cypress wood essential oil, respectively.

[0108] S2. Take 100g each of lemon, argan oil, sesame seeds, jojoba seeds, and black cumin seeds, wash them thoroughly, and extract them separately using a cold pressing method. When pressing using a physical machine, maintain the temperature at 7℃ and isolate them from oxygen and light. Then, use a centrifuge to separate the oils, filter the extracted products, and collect the second filtrate to obtain lemon essential oil, argan oil, sesame oil, jojoba oil, and black cumin seed oil, respectively.

[0109] S3. The products obtained in steps S1 and S2 are combined as follows: 5 parts by weight of cedarwood essential oil, 3 parts by weight of peppermint essential oil, 5 parts by weight of rosemary essential oil, 3 parts by weight of lavender essential oil, 1 part by weight of sandalwood essential oil, 5 parts by weight of cypress essential oil, 1 part by weight of bitter orange leaf essential oil, 5 parts by weight of ginger essential oil, 1 part by weight of yellow cypress essential oil, 1 part by weight of lemon essential oil, 15 parts by weight of argan oil, 65 parts by weight of sesame oil, 30 parts by weight of jojoba oil, and 10 parts by weight of black cumin seed oil.

[0110] Mix thoroughly to obtain the compound essential oil of Example 2, and place it in a brown bottle for later use.

[0111] Example 3

[0112] S1. Take 100g each of cedarwood, peppermint, rosemary, lavender, sandalwood, arborvitae, petitgrain, ginger, and cypress wood, wash and grind them. Then, add them separately to a distillation apparatus, add 500ml of water, and perform steam distillation extraction at 30℃. After 250min, remove the heat, collect the distillation products, and obtain cedarwood essential oil, peppermint essential oil, rosemary essential oil, lavender essential oil, sandalwood essential oil, arborvitae essential oil, petitgrain essential oil, ginger essential oil, and cypress wood essential oil, respectively.

[0113] S2. Take 100g each of lemon, argan oil, sesame seeds, jojoba seeds, and black cumin seeds, wash them thoroughly, and extract them separately using a cold pressing method. When pressing using a physical machine, maintain the temperature at 7℃ and isolate them from oxygen and light. Then, use a centrifuge to separate the oils, filter the extracted products, and collect the second filtrate to obtain lemon essential oil, argan oil, sesame oil, jojoba oil, and black cumin seed oil, respectively.

[0114] S3. The products obtained in steps S1 and S2 are combined as follows: 5 parts by weight of cedarwood essential oil, 3 parts by weight of peppermint essential oil, 5 parts by weight of rosemary essential oil, 3 parts by weight of lavender essential oil, 1 part by weight of sandalwood essential oil, 5 parts by weight of cypress essential oil, 1 part by weight of bitter orange leaf essential oil, 5 parts by weight of ginger essential oil, 1 part by weight of yellow cypress essential oil, 1 part by weight of lemon essential oil, 15 parts by weight of argan oil, 65 parts by weight of sesame oil, 30 parts by weight of jojoba oil, and 10 parts by weight of black cumin seed oil.

[0115] Mix thoroughly to obtain the compound essential oil of Example 3, and place it in a brown bottle for later use.

[0116] Example 4

[0117] S1. Take 100g each of cedarwood, peppermint, rosemary, lavender, sandalwood, arborvitae, petitgrain, ginger, and cypress wood, wash and grind them. Then, add them separately to a distillation apparatus, add 550ml of water, and perform steam distillation extraction at 30℃. After 330min, remove the heat, collect the distillation products, and obtain cedarwood essential oil, peppermint essential oil, rosemary essential oil, lavender essential oil, sandalwood essential oil, arborvitae essential oil, petitgrain essential oil, ginger essential oil, and cypress wood essential oil, respectively.

[0118] S2. Take 100g each of lemon, argan oil, sesame seeds, jojoba seeds, and black cumin seeds, wash them thoroughly, and extract them separately using a cold pressing method. When pressing using a physical machine, maintain the temperature at 8℃ and isolate them from oxygen and light. Then, use a centrifuge to separate the oils, filter the extracted products, and collect the second filtrate to obtain lemon essential oil, argan oil, sesame oil, jojoba oil, and black cumin seed oil, respectively.

[0119] S3. The products obtained in steps S1 and S2 are combined as follows: 5 parts by weight of cedarwood essential oil, 3 parts by weight of peppermint essential oil, 5 parts by weight of rosemary essential oil, 3 parts by weight of lavender essential oil, 1 part by weight of sandalwood essential oil, 5 parts by weight of cypress essential oil, 1 part by weight of bitter orange leaf essential oil, 5 parts by weight of ginger essential oil, 1 part by weight of yellow cypress essential oil, 1 part by weight of lemon essential oil, 15 parts by weight of argan oil, 65 parts by weight of sesame oil, 30 parts by weight of jojoba oil, and 10 parts by weight of black cumin seed oil.

[0120] Mix thoroughly to obtain the compound essential oil of Example 4, and place it in a brown bottle for later use.

[0121] Table 1. Components of the compound essential oils in each example.

[0122] In summary, the compound essential oil of the present invention contains the following components in parts by weight: oleic acid 25-40 parts, linoleic acid 20-35 parts, palmitic acid 1-10 parts, cis-11-eicosenoic acid 1-10 parts, stearic acid 1-10 parts, cis-13-docosaen-1-ol 1-10 parts, β-cedrene 1-10 parts, cis-9-eicoseno-1-ol 1-10 parts, thujone 0.5-5 parts, α-pinene 0.5-5 parts, gingerene 0.5-5 parts, erucic acid 0.5-5 parts, limonene 0.1-1 part, α-cedrene 0.1- 1 part, camphene 0.1-1 part, linalool 0.1-1 part, γ-cedrene 0.1-1 part, eucalyptol 0.1-1 part, camphor 0.1-1 part, menthol 0.1-1 part, α-curcumene 0.1-0.8 parts, eicosanoic acid 0.1-0.8 parts, fentanyl 0.1-0.8 parts, β-sesquiphellandrene 0.1-0.8 parts, (+)-δ-penicillin 0.1-0.8 parts, menthone 0.1-0.8 parts, β-bifasonene 0.1-0.8 parts, β-pinene 0.1-0.7 parts, borneol 0.1-0.7 parts. 0.1-0.7 parts of verbenatenene, 0.1-0.7 parts of sapinene, 0.1-0.7 parts of myrcene, 0.1-0.7 parts of trans-caryophyllene, 0.01-0.5 parts of p-cymene, 0.01-0.5 parts of γ-terpinene, 0.01-0.5 parts of β-phellandrene, 0.01-0.5 parts of α-farnesene, 0.01-0.5 parts of linolenic acid, 0.01-0.2 parts of α-santalene, 0.01-0.2 parts of 4-terpinenol, 0.01-0.2 parts of isomenthone, and 0.01-0.2 parts of linalyl acetate. 0.01-0.2 parts of 1,4-cedradine, 0.01-0.2 parts of docosuccinic acid, 0.01-0.2 parts of longifolene, 0.01-0.2 parts of terpinene, 0.01-0.2 parts of 3-thujone, 0.01-0.2 parts of (-)-β-santalene, 0.01-0.2 parts of black antin, 0.01-0.1 parts of fennel, 0.01-0.1 parts of trans-α-bergamot glycerene, 0.01-0.1 parts of palmitoleic acid, 0.01-0.1 parts of β-elemene, and 0.01-0.1 parts of trans-β-ocimene. 0.01-0.1 parts of α-terpinene, 0.01-0.1 parts of epi-β-santalene, 0.01-0.1 parts of β-ocimene, 0.01-0.1 parts of trans-β-farnesene, and 0.01-0.1 parts of tricycloene.

[0123] Further, it comprises the following components in parts by weight: oleic acid 28-35 parts, linoleic acid 20-30 parts, palmitic acid 5-10 parts, cis-11-eicosenoic acid 5-10 parts, stearic acid 2-8 parts, cis-13-docosaen-1-ol 1-5 parts, β-cedrene 1-5 parts, cis-9-eicosenoen-1-ol 1-5 parts, thujone 0.5-3 parts, α-pinene 0.5-3 parts, gingerene 0.5-2 parts, erucic acid 0.5-2 parts, limonene 0.5-1 part, α-cedrene 0.5-1 part, camphene 0.4-0.9 parts, linalool 0.2-0.8 parts, and γ-cedrene 0.2-0. 0.8 parts, eucalyptol 0.2-0.8 parts, camphor 0.2-0.5 parts, menthol 0.2-0.5 parts, α-curcumene 0.2-0.5 parts, eicosanoic acid 0.2-0.5 parts, fentanyl 0.2-0.5 parts, β-sesquiphellandrene 0.2-0.5 parts, (+)-δ-piperazine 0.2-0.5 parts, menthone 0.2-0.5 parts, β-bimethene 0.2-0.5 parts, β-pinene 0.1-0.4 parts, borneol 0.1-0.4 parts, verbenatenone 0.1-0.4 parts, sapinene 0.1-0.4 parts, myrcene 0.1-0.4 parts, trans-caryophyllene 0.1-0. 0.4 parts, p-cymene 0.05-0.3 parts, γ-terpinene 0.05-0.3 parts, β-phellandrene 0.05-0.3 parts, α-farnesene 0.05-0.3 parts, linolenic acid 0.05-0.3 parts, α-santalene 0.05-0.15 parts, 4-terpinenol 0.05-0.15 parts, isomenthone 0.05-0.15 parts, linalyl acetate 0.05-0.15 parts, 1,4-cedradine 0.05-0.15 parts, docosuccinic acid 0.05-0.15 parts, longifolene 0.05-0.15 parts, terpinene 0.05-0.15 parts, 3-thujone 0. 0.05-0.15 parts, (-)-β-santalene 0.05-0.15 parts, black antin 0.05-0.15 parts, fennel 0.01-0.08 parts, trans-α-bergamot glycerene 0.01-0.07 parts, palmitoleic acid 0.01-0.07 parts, β-elemene 0.01-0.07 parts, trans-β-ocimene 0.01-0.07 parts, α-terpinene 0.01-0.06 parts, epi-β-santalene 0.01-0.06 parts, β-ocimene 0.01-0.06 parts, trans-β-farnesene 0.01-0.05 parts, tricycloene 0.01-0.05 parts.

[0124] Effect Example

[0125] Population testing

[0126] 1. See Table 1 for the ingredient list.

[0127] 2. Human trials:

[0128] Forty volunteers with hair loss problems were recruited. Their hair loss was not cyclical and was considered pathological. The patients were randomly divided into four groups of ten each. Groups 1 through 10 used the compound essential oils described in Examples 1-4 of this invention. The essential oils were applied to the scalp and massaged until absorbed. Use was continued for two months, once daily, 1g each time.

[0129] 2.1 Product Name: A Compound Essential Oil for Hair Growth and Hair Loss Prevention and its Preparation Method

[0130] 2.2 How to use: Take 1g of compound essential oil in the palm of your hand, apply it to the scalp, and massage until the essential oil is absorbed.

[0131] 2.3 Usage period: 60 days

[0132] 2.4 Subject population: Hair loss patients who have hair loss problems and still have normal hair follicles that are visible to the naked eye.

[0133] 2.5 Result Detection Method: Based on user feedback.

[0134] 2.6 Test results: See Figures 1-8.

[0135] The results above show that, in the test subjects, hair loss was effectively improved after using the compound essential oil for hair growth and hair loss prevention provided by this invention. At the same time, no obvious side effects were observed in the test subjects.

[0136] The above description is merely an example of the embodiments of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the technical principles of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

[0137] Evaluation of the hair regrowth effect of compound essential oils in a mouse model of hair loss

[0138] 1. Materials and Methods

[0139] 1.1 Experimental Animals

[0140] 24 male C57 mice, 20g each.

[0141] 1.2 Experimental reagents and materials

[0142] The compound essential oil prepared in Example 1; androgens; commercially available olive oil; a commercially available brand of hair removal cream; 1.3 Animal grouping and administration

[0143] C57 mice were randomly divided into four groups (blank control group, model control group, compound essential oil group, and positive control group). 0.3g of androgen was placed in a 5mL centrifuge tube, and 3ml of olive oil was added. The mixture was repeatedly blown and stirred until the androgen dissolved. Male C57 mice were injected with androgen (200μL / mouse) every evening at 9 PM. The outer layer of hair was shaved off with clippers, and then depilatory cream was applied. After 1.5 minutes, the back of the mouse was wiped clean with a cotton ball soaked in distilled water. This process was repeated until all the hair on the back was removed. (Androgen was injected every evening.)

[0144] A. Blank control group: Intraperitoneal injection of an equal volume of physiological saline.

[0145] B. Model control group: Intraperitoneal injection of 0.1 mL (1 mg) of dihydrotestosterone solution at a concentration of 10 mg / mL.

[0146] C. Compound essential oil group: The drug was administered while modeling the hair removal process. 5% essential oil was applied topically to the hair removal area once a day.

[0147] D. Positive control group: During the modeling process, the patient was treated with 5% minoxidil topically once a day in the hair removal area.

[0148] 2. Experimental Results and Analysis

[0149] 2.1 Establishment of a mouse model of androgenetic alopecia

[0150] A mouse model of androgenetic alopecia was successfully established, as shown in Figure 10.

[0151] 2.2 Therapeutic effect of compound essential oils on androgenetic alopecia model

[0152] ①After applying the essential oil for one week, the hair growth essential oil group of this invention showed significantly more hair growth than the model control group and better hair growth than the positive control group, as shown in Figure 11;

[0153] ② Two weeks after applying the essential oil, large areas of hair appeared on the backs of all three groups, with the compound essential oil group showing the most obvious effect, as shown in Figure 12.

[0154] ③ Hair length was observed, with the compound essential oil group showing the best growth, followed by the positive control group (minoxidil tincture), as shown in Figure 13.

[0155] ④ The hair growth rate shows that the hair growth essential oil group of the present invention has the highest hair growth rate, as shown in Figure 14.

[0156] 2.3 Safety Evaluation

[0157] Safety assessments showed that the compound essential oil was non-toxic and safer than minoxidil solution.

[0158] ①Liver

[0159] Aspartate aminotransferase (AST): It is mainly distributed in the myocardium, followed by the liver, skeletal muscle, and kidneys. Normally, serum AST levels are low, but when corresponding cells are damaged, AST from the cytoplasm is released into the blood, thus increasing its serum concentration. The results are shown in Figure 15.

[0160] Alanine aminotransferase (ALT): Under normal circumstances, the concentration of alanine aminotransferase in human blood is low. When liver cells are damaged, the concentration of alanine aminotransferase in serum will increase. The results are shown in Figure 16.

[0161] Albumin (ALB): an indicator of liver function. The results showed no statistically significant difference, indicating no significant risk. The results are shown in Figure 17.

[0162] Alkaline phosphatase (ALP): Clinically, ALP measurement is mainly used for the diagnosis and differential diagnosis of diseases of the skeletal and hepatobiliary systems. ALP results show that the hair growth essential oil has good safety profile, as shown in Figure 18.

[0163] ②Kidney

[0164] Blood urea nitrogen (BUN): BUN levels will increase in decompensated renal insufficiency. There was no statistically significant difference between the compound essential oil group and the blank control group, as shown in Figure 19.

[0165] Serum uric acid (UA): a biochemical indicator for diagnosing gout. The results showed no statistically significant difference, as shown in Figure 20.

[0166] ③ Myocardial acid kinase (CK): An important indicator for diagnosing myocardial infarction. The results showed no significant statistical difference, as shown in Figure 21.

[0167] ④ Glucose (GLU): A diagnostic indicator for metabolic diseases such as diabetes. The results showed no statistically significant difference. (See Figure 22).

[0168] ⑤ Triglycerides (TG): One of the diagnostic indicators of hyperlipidemia. The results showed no statistically significant difference. (See Figure 23).

[0169] ⑥ Lactate dehydrogenase (LDH): This is one of the indicators for assessing cardiac, hepatic, and renal function. The results showed no significant statistical difference, as shown in Figure 24.

[0170] 3. Conclusion

[0171] The hair in the compound essential oil group was longer than that in the model control group and the positive control group (minoxidil solution); the effect was achieved earlier; the hair density was denser; and the hair growth rate was higher. In the positive control group, serum ALP was significantly elevated, suggesting that minoxidil solution may have potential hepatotoxicity. Compared with the positive control group, ALP was lower in the compound essential oil group, suggesting that the compound essential oil has higher safety.

[0172] In summary, the hair growth essential oil of this invention can improve androgenetic alopecia, with a significant hair growth effect, and has the advantages of being more effective, faster-acting, and safer than the positive control group (minoxidil solution).

Claims

1. A compound essential oil for hair growth and hair loss prevention, characterized in that, The ingredients include: 1-5 parts by weight of cedarwood essential oil, 0.5-3 parts by weight of peppermint essential oil, 1-5 parts by weight of rosemary essential oil, 0.5-3 parts by weight of lavender essential oil, 0.1-1 parts by weight of sandalwood essential oil, 1-5 parts by weight of cypress essential oil, 0.01-1 parts by weight of bitter orange leaf essential oil, 1-5 parts by weight of ginger essential oil, 0.01-1 parts by weight of yellow cypress essential oil, 0.01-1 parts by weight of lemon essential oil, 5-15 parts by weight of argan oil, 40-65 parts by weight of sesame oil, 10-30 parts by weight of jojoba oil, and 1-10 parts by weight of black cumin seed oil.

2. The compound essential oil according to claim 1, wherein the raw materials include: 1 part by weight of cedarwood essential oil, 0.5 part by weight of peppermint essential oil, 1 part by weight of rosemary essential oil, 0.5 part by weight of lavender essential oil, 0.1 part by weight of sandalwood essential oil, 1 part by weight of arborvitae essential oil, 0.01 part by weight of bitter orange leaf essential oil, 1 part by weight of ginger essential oil, 0.01 part by weight of yellow cypress essential oil, 0.01 part by weight of lemon essential oil, 5 parts by weight of argan oil, 40 parts by weight of sesame oil, 10 parts by weight of jojoba oil, and 1 part by weight of black cumin seed oil.

3. The compound essential oil according to claim 1, wherein the raw materials are: 5 parts by weight of cedarwood essential oil, 3 parts by weight of peppermint essential oil, 5 parts by weight of rosemary essential oil, 3 parts by weight of lavender essential oil, 1 part by weight of sandalwood essential oil, 5 parts by weight of cypress essential oil, 1 part by weight of bitter orange leaf essential oil, 5 parts by weight of ginger essential oil, 1 part by weight of yellow cypress essential oil, 1 part by weight of lemon essential oil, 15 parts by weight of argan oil, 65 parts by weight of sesame oil, 30 parts by weight of jojoba oil, and 10 parts by weight of black cumin seed oil.

4. The method for preparing the compound essential oil for hair growth and hair loss prevention according to any one of claims 1-3, comprising the following steps: S1. Take cedarwood, peppermint, rosemary, lavender, sandalwood, arborvitae, bitter orange leaf, ginger and yellow cypress, and extract them by steam distillation. S2. Extract lemon, argan oil, sesame, jojoba seeds, and black cumin seeds using a cold pressing method; S3. Mix the essential oils obtained in the above steps according to the stated weight proportions to obtain a compound essential oil.

5. The preparation method according to claim 4, wherein, The essential oils are derived from the leaves and branches of Atlantic cedar and rosemary, the plant of peppermint, the rhizome of ginger, the flower of lavender, the leaves of cypress and bitter orange, the wood of sandalwood and yellow cypress, the peel of lemon, the kernel of argan oil, and the seeds of sesame, jojoba, and black cumin.

6. The preparation method according to claim 4, comprising the following steps: S1. Take cedarwood, peppermint, rosemary, lavender, sandalwood, arborvitae, bitter orange leaf, ginger, and cypress wood. Wash them clean and crush them separately to obtain corresponding plant solid fragments. Use steam distillation to extract each plant solid fragment separately. Filter the extracted products and collect the filtrate to obtain cedarwood essential oil, peppermint essential oil, rosemary essential oil, lavender essential oil, sandalwood essential oil, arborvitae essential oil, bitter orange leaf essential oil, ginger essential oil, and cypress wood essential oil, respectively. S2. Take lemon, argan oil, sesame, jojoba seeds and black cumin seeds, wash them clean and crush them, and then extract them by cold pressing to obtain lemon essential oil, argan oil, sesame oil, jojoba oil and black cumin seed oil respectively. S3. Mix the cedarwood essential oil, peppermint essential oil, rosemary essential oil, lavender essential oil, sandalwood essential oil, cypress essential oil, bitter orange leaf essential oil, ginger essential oil, yellow cypress essential oil, lemon essential oil, argan oil, sesame oil, jojoba oil, and black cumin seed oil obtained in the above steps according to the stated weight proportions to obtain a compound essential oil.

7. The preparation method according to claim 4, wherein when extracting each plant essential oil by steam distillation in S1, the mass ratio of water to each pulverized plant solid fragment is 4:1-6:

1.

8. The preparation method according to claim 4, wherein in S2, the specific process of cold pressing to extract plant essential oil is as follows: physical pressing by machine → separation of oil by centrifuge → filtration of the extracted product and collection of the second filtrate, the obtained second filtrate being the corresponding essential oil.

9. The preparation method according to claim 4, wherein in S2, when extracting plant essential oils by cold pressing, the extraction should be carried out at 5-10°C.

10. The use of the compound essential oil according to any one of claims 1-3 in the preparation of hair growth and anti-hair loss products.

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