A natural ointment for wound healing: combining sheep tail fat, honey, beeswax, and herbal ingredients

Abstract

Wound care is the third most costly medical challenge globally, following cancer and cardiovascular diseases. With the growing prevalence of various types of wounds, there is a continuous and increasing need for rapid and effective treatments, particularly those based on natural compounds. Beyond the support of the World Health Organization (WHO) for such approaches, the use of natural products (of both plant and animal origin) that are well tolerated by patients and free of significant side effects is now a clear priority. Iran, with its diverse ecological and climatic conditions, is uniquely positioned as a major producer of medicinal plants and various natural products. The primary goal of this invention is to utilize natural ingredients—including sheep tail fat, beeswax, wild pistachio resin, castor oil, coconut oil, olive oil, turmeric, and honey—to develop an ointment formulation designed to promote the healing of various skin wounds, prevent keloid formation, and provide antibacterial and antifungal effects at the site of application. Key features and innovations of this ointment formulation compared to similar domestic and international products include its carefully selected natural ingredients, lack of side effects associated with conventional chemical drugs, the ready availability and local sourcing of most components, and its very low production cost.

Description

A Natural Ointment for Wound Healing: Combining Sheep Tail Fat, Honey, Beeswax, and Herbal Ingredients

[0001] This invention relates to an ointment formulation made from animal-based ingredients and plant-derived active compounds. The resulting blend is designed to promote the healing of various types of wounds including cuts, diabetic ulcers, infected wounds, and first- and second-degree burns, while also relieving associated pain. Additionally, it offers antibacterial, antifungal, and anti-abscess effects at the site of application.

[0002] The skin is an especially vital organ for humans, serving to prevent moisture loss, block microbial invasion, and stop bleeding. Since ancient times, physicians in Egypt, Greece, India, and Europe have relied on medicinal plants and various natural substances in traditional medicine to treat and heal wounds. Today, a range of chemical and synthetic drugs is used to accelerate wound healing. However, issues such as limited effectiveness, drug resistance, high costs, and side effects have all led to growing patient interest in natural and herbal products as safer and more affordable alternatives.

[0003] A wound is defined as a disruption of the normal structure and function of the skin and underlying soft tissue. Maintaining the integrity of the skin is essential for preventing dehydration, bleeding, and the entry of microorganisms. Skin wounds can result from various causes, including physical, chemical, and biological injuries, and are generally classified as either acute or chronic based on their healing time and characteristics.

[0004] Acute wounds, such as burns and cuts, are typically superficial and tend to heal completely within 8 to 12 weeks. In contrast, chronic wounds—like venous or arterial leg ulcers, diabetic foot ulcers, and pressure sores—are usually associated with underlying physiological disorders that impair the healing process. These wounds heal slowly and often take longer than 12 weeks to resolve.

[0005] One of the key goals in medicine is to achieve faster wound healing with fewer side effects. Improving the speed of wound healing has significant economic and health benefits. Faster healing reduces the risk of wound infection and accelerates the overall recovery process.

[0006] Wound healing itself is a complex, multi-stage process that must proceed in a specific sequence and within defined timeframes. Each phase requires the right duration and intensity to be effective; if this balance is disrupted, the wound may become chronic. Moreover, the rate of wound healing depends on many factors, including wound size, blood supply to the area, the presence of foreign bodies or microorganisms, the patient’s age and overall health, and nutritional status..

[0007] In patent file number IR / 94407, an herbal ointment for the treatment of diabetic wounds, burns, and damaged skin has been registered. This invention describes the formulation of an ointment using plant-based ingredients to support healing in diabetic wounds, burns, and other skin injuries. The herbal product is made from a blend of ingredients such as lotus jujube (cedar), borage, honey, olive, henna, sesame, iris, beet, aloe vera, St. John’s wort, turmeric, rosemary, calendula, and animal fat. The main difference between that formulation and our proposed design lies in the raw materials used. The patented formulation relies on a mix of specific plant ingredients that can be more expensive and sometimes difficult to source compared to the ingredients in our proposed ointment formulation.

[0008] In patent file number IR / 80303, a burn ointment intended for the complete healing of burn wounds, pressure ulcers, and other types of wounds has been registered. This invention describes an ointment formulated with both plant- and animal-derived ingredients to achieve thorough healing of burns, pressure sores, and other wounds. The formulation includes animal fat, egg, castor oil, beeswax, turmeric, vegetable oil, Ozmak extract, and chamomile extract. The difference between that formulation and our proposed design lies in the raw materials used. The patented formula relies on specific plant and animal ingredients that can be more costly and difficult to source in large quantities compared to the ingredients chosen for our proposed ointment formulation.

[0009] In patent file number IR / 70721, an herbal ointment effective for treating surgical wounds based on chloroform extract of mallow has been registered. This invention describes an herbal ointment formulated specifically with chloroform extract of mallow for surgical wound treatment. The difference between that invention and our proposed formulation lies in the ingredients used. The patented ointment relies solely on mallow chloroform extract, whereas our proposed formulation uses a broader combination of ingredients.

[0010] In patent file number IR / 70720, the preparation and processing of a herbal ointment for treating surgical wounds in diabetic patients has been registered. This herbal ointment is formulated using hydroalcoholic extracts of mallow and Persian pomegranate blossom (Gulnar Farsi). Compared to patent number IR / 70721, this formulation includes both mallow and Persian pomegranate blossom extracts rather than just mallow. The difference between that invention and our proposed formulation lies in the process and raw materials used to make the ointment.

[0011] In patent file number IR / 98186, a herbal topical medicine (ointment) for diabetic wound healing has been registered. This ointment combines extracts of the plantsArnebiaeuchromaand gum of Astragalus (Ven gum),produced for the first time in Iran. The difference between that invention and our proposed formulation lies in the process and raw materials used. Additionally, sourcing the ingredients specified in that patent can be somewhat challenging.

[0012] In patent application number IR / 139750140003008154, an ointment containing honey and extracts of black seed, propolis, and senna leaf was registered for the treatment of diabetic ulcers. The difference between that invention and our proposed formulation is in the process and raw materials used, as well as the range of therapeutic effects.

[0013] In patent file number IR / 139950140003003010, a hydroalcoholic gel containing astragalus plant extract with analgesic and wound-healing properties was registered. The difference between that invention and our proposed formulation lies in the process and raw materials used,as well as thespectrum of therapeutic effects.Summary of the Invention

[0014] This invention introduces a completely natural ointment designed to speed up the healing of different kinds of skin wounds, from minor cuts and burns to diabetic and infectious ulcers. The formula brings together sheep tail fat, mastic gum (Pistacia atlantica,kurdica), beeswax, castor oil, olive oil, coconut oil, turmeric, and honey. Together, these ingredients offer powerful anti-inflammatory, antibacterial, antifungal, and healing benefits, without any synthetic chemicals. In animal studies, the ointment facilitated faster and more effective wound healing compared to standard treatments. It’s cost-effective, made from easily accessible materials, and ideal for mass production in the pharmaceutical Industries.

[0015] Wound care and treatment represent a significant medical and economic issue in modern healthcare. Impaired wound healing leads to considerable mortality worldwide. According to a 2013 study, skin wounds rank as the third most costly medical condition globally, after cancer and cardiovascular diseases, and therefore account for a substantial share of biomedical research.

[0016] With the rising prevalence of various types of wounds such as diabetic ulcers the need for rapid and effective treatment is steadily increasing.

[0017] Currently, a range of chemical drugs, including gentamicin, mupirocin, phenytoin, and nitrofurazone, are used to accelerate healing. However, challenges such as limited efficacy, drug resistance, side effects, and high costs have driven many patients to seek natural and herbal alternatives. As a result, research into medicinal plants and natural products has become a priority area both globally and in Iran.

[0018] Fortunately, Iran is rich in diverse natural resources—such as animal-derived oils and medicinal plants. These natural products tend to have fewer side effects compared to synthetic drugs, are generally well-tolerated by patients, and when locally sourced help reduce foreign currency outflows.

[0019] Today, various plant- and animal-based compounds are already used to promote wound healing. In line with this trend, the proposed project aims to develop an ointment formulation that uses natural animal- and plant-derived ingredients. Through a specific preparation process, this ointment is designed to relieve pain, promote healing, and reduce scarring while also offering antibacterial, antifungal, and even anti-abscess effects at the site of application.

[0020] Moreover, the production cost of this ointment is expected to be significantly lower than both imported and domestic alternatives, thanks to the availability and local sourcing of most ingredients, making it highly suitable for industrial-scale production.

[0021] This invention describes an ointment formulation with both animal- and plant-based ingredients, produced through a specific process to ensure ease of use. This product accelerates the healing of various types of skin wounds without leavingkeloid scars at the injury site. In addition to treating infected wounds, it also helps prevent new infections from developing. Moreover, this ointment formulation is designed to reduce pain in the wound area.

[0022] Sheep tail fat oil is rich in nutrients, various vitamins, and fatty acids. It typically contains around 29.3% to 54.8% saturated fatty acids and approximately 45.9% to 65.8% unsaturated fatty acids. A significant portion of its saturated fats consists of short-chain fatty acids. Research has shown the beneficial effects of certain nutrients—such as arginine, glutamine, vitamin C, and fatty acids—on the wound-healing process. In particular, studies have highlighted the positive role of unsaturated fatty acids in wound repair. Multiple investigations have demonstrated that polyunsaturated fatty acids (PUFAs) play a crucial role in tissue regeneration. Specifically, unsaturated fatty acids help reduce the production of pro-inflammatory cytokines (such as IL-1, IL-6, and TNF-α) and their downstream products (including leukotrienes, prostaglandins, and thromboxane’s). This gives them notable anti-inflammatory properties. Additionally, one study reported the beneficial effects of sheep fat in wound healing. The results showed that the combined use of sheep fat oil and honey helped protect wounds from infection, with the oil acting as a barrier against microbial invasion.

[0023] Saqez is a resin extracted from the wild pistachio tree(Pistacia atlantica), also known locally as Beneh, Pistacia atlantica, kurdica, Kolkhunak, or simply Saqez. In traditional Iranian medicine, the oil derived from Saqez fruit has been used for treating wounds, inflammation, and other conditions. GC-MS analysis of Saqez oil has shown that its major components include unsaturated fatty acids (PUFAs) such as linoleic acid (38.1%), oleic acid (36.9%), and stearic acid (3.8%). Fatty acids are known to have numerous skin health benefits and play an important role in wound healing by regulating epithelialization. One study demonstrated that Saqez oil reduced neutrophil levels at the wound site, contributing to anti-inflammatory effects after 21 days of treatment. Another study found that Saqez oil facilitated wound contraction and epithelial layer formation in rabbits. Additionally, Saqez oil is rich in phenolic compounds, giving it strong antioxidant properties. Other research has also highlighted the resin’s effective wound-healing properties.

[0024] Beeswax is secreted by worker bees. Gas chromatography–mass spectrometry (GC-MS) analysis has identified various components in beeswax, including alkanes, alkynes, free fatty acids, monoesters, diesters, and hydroxy monoesters. These compounds give beeswax its natural moisturizing and emollient properties. Beeswax can also contain propolis and pollen oil. Propolis itself is rich in phenolic acids, esters, flavonoids, and other organic compounds. When present in beeswax, it provides strong antioxidant, anti-inflammatory, and antimicrobial properties, making it highly beneficial for treating skin conditions and promoting wound healing. Beeswax also absorbs water and hydrates the wound environment, helping to accelerate healing. Research has documented many therapeutic effects of beeswax, including its anti-inflammatory properties, wound-healing ability, effectiveness in treating second-degree burns, antifungal activity, relief of oral and lip lesions, and anti-itch effects. One study demonstrated that a combination of beeswax (10 g), olive oil (10 cc), and butter (10 g) applied via an impregnated bandage significantly improved healing in an animal model of second-degree burns. The results suggested that this mixture promoted fibroblast activity and keratinization through the induction of growth factors such as TGF-β1 and VEGF-α, leading to faster burn wound regeneration in rats. Today, beeswax remains a widely used and cost-effective ingredient in many skincare products and topical formulations

[0025] Castor oil is extracted from the seeds of the castor plant (Ricinus communis). It has a wide range of applications in medicine, industry, and pharmaceuticals, and is used in food, medicine, and skincare products. Castor oil is rich in triglycerides, with ricinoleic acid—a unique monounsaturated fatty acid—being its primary component. Research has shown that ricinoleic acid is well-known for its moisturizing properties. By creating a moist environment and preventing the wound from drying out, castor oil can help promote wound healing. Additionally, ricinoleic acid may help reduce skin inflammation and relieve wound pain. It also exhibits antibacterial properties that help prevent bacterial growth on the skin. Regular use of castor oil has been shown to reduce acne, pimples, and other skin inflammations. However, castor oil is typically not used alone for treating skin disorders; it is often combined with other ingredients in formulations designed for skin care and wound healing

[0026] Olive oil is extracted from the fruit of the olive tree (Olea europaea). Its main and most abundant component is oleic acid, a monounsaturated fatty acid. Olive oil also contains smaller amounts of other fatty acids and polyphenols. The high content of oleic acid and polyphenols gives olive oil strong anti-inflammatory and antioxidant properties. These pharmacological effects play a significant role in the wound-healing process. One study found that topical application of olive oil on diabetic wounds in rats accelerated wound closure and promoted angiogenesis (formation of new blood vessels). A clinical study on patients with grade 1 and 2 diabetic foot ulcers also showed that using olive oil helped speed up the healing process. Beyond topical use, another study reported that oral consumption of olive oil in patients with second-degree burns reduced hospital stay duration and accelerated wound healing. These beneficial effects are thought to be related to the stimulation of the Nrf2 pathway by the polyphenolic compounds in olive oil. Additionally, other research has used olive oil as a matrix material in chitosan nanocomposites, showing enhanced antibacterial activity of the resulting films against Escherichia coli, Staphylococcus aureus, and Micrococcus luteus. However, relatively few studies have specifically examined olive oil’s effects on the healing of acute wounds, such as surgical wounds

[0027] Coconut oil is extracted from the fruit of the coconut tree (Cocos nucifera). For centuries, it has been used in Ayurvedic medicine to treat skin disorders, including wound healing and microbial infections. Analysis of coconut oil reveals that it is rich in medium-chain fatty acids (MCFAs, C6–C12). The most abundant fatty acid in coconut oil is lauric acid, which is well known for its antimicrobial properties. Other fatty acids present include oleic acid, caproic acid, palmitic acid, and more, all contributing to its ability to combat microbial infections. Beyond fatty acids, coconut oil also contains polyphenols (such as catechin, ferulic acid, p-coumaric acid, and caffeic acid), phenolic acids, unidentified flavonoids, as well as vitamin E and vitamin A. These compounds give coconut oil strong antioxidant activity, which plays a crucial role in supporting wound healing. One study specifically highlighted coconut oil’s wound-healing properties. The results showed that applying 0.5 to 1 cc of coconut oil daily accelerated epithelialization and improved wound healing in rats. Histopathological findings also indicated increased fibroblast proliferation and new blood vessel formation (angiogenesis) in the treated animals.

[0028] Turmeric powder is derived from the rhizome of the plantCurcuma longa. Its key bioactive compounds are yellow pigments known as curcuminoids, which make up about 3–5% of turmeric’s composition. The primary curcuminoids are polyphenolic compounds such as curcumin and desmethoxycurcumin. Research has documented turmeric’s anti-inflammatory, antioxidant, and antibacterial properties. Curcumin, the main active component, has been especially well studied for its wound-healing benefits. Studies have shown that curcumin can inhibit the production of pro-inflammatory factors involved in skin wounds, including interleukins (IL-1, IL-6), tumor necrosis factor-alpha (TNF-α), and nuclear factor kappa B (NF-κβ). By reducing these inflammatory mediators, curcumin helps promote wound healing. In addition to its anti-inflammatory action, curcumin has demonstrated antioxidant properties and the ability to stimulate collagen production in the skin, both of which are critical for effective wound repair. Further research has shown that curcumin accelerates the overall wound-healing process by enhancing tissue granulation, regeneration, and wound contraction across multiple healing stages.

[0029] Previous studies have reported that honey is a safe, natural substance with proven effectiveness in inhibiting bacterial growth and treating a wide variety of wounds. These include burns, abrasions, diabetic boils (skin abscesses related to diabetes), malignancies, leprosy, fistulas, leg ulcers, traumatic sores, cervical and varicose ulcers, amputations, dehisced abdominal wounds, septic and surgical wounds, cracked nipples, and abdominal wall ulcers. Natural honey is composed of approximately 82% carbohydrates and contains a wide range of bioactive compounds, including flavonoids, organic acids, phenolic acids, vitamins, minerals, enzymes, carbohydrates, proteins, and antioxidants. All of these components contribute to honey’s significant biological and therapeutic potential, especially in wound healing.

[0030] The primary sugars in honey, in order of abundance, include fructose (38.2%), glucose (31.2%), various disaccharides and trisaccharides, higher saccharides (around 9%), and sucrose (0.7–1%). These sugars and other active compounds play a crucial role in the wound-healing process. Honey’s natural viscosity and gel-like consistency create a protective layer over the wound, preventing bacterial entry and maintaining moisture balance to avoid dehydration. Studies have shown that honey’s high sugar content increases osmotic pressure gradients, drawing fluid from underlying tissue layers toward the surface, which promotes healing. Additionally, honey’s low water activity prevents bacterial growth at the wound site, while facilitating the delivery of nutrients and oxygen to deeper wound tissues. Its acidic pH enhances tissue oxygenation, and its flavonoids and organic acids help prevent cell damage caused by oxidative stress. Another important antimicrobial mechanism of honey is its ability to produce hydrogen peroxide, which sterilizes the wound site and stimulates the production of vascular endothelial growth factor (VEGF)—a key player in promoting new blood vessel formation and supporting tissue regeneration.

[0031] Table 1 illustrates ingredients of the formulation and indicates the amount and percentage of each component.

[0032] Table 1: The formulation and indicates the amount and percentage of each componentPercentage (%)Amount (gr)CombinationsNo.25.16615.1Sheep tail fat or Rump oi18.3335Pistacia atlantica28.3335Beeswax35.53.3Castor oil45.53.3Olive oil55.53.3Coconut oil616.66610Turmeric72515Honey810060SumOperational and Technical Description

[0033] The base formulation for the ointment is prepared using the specified ingredients. Sheep tail fat is first chopped into small pieces. Depending on the volume, one or two cups of water are added, and the mixture is gently heated until the fat is completely melted. The oil is extracted in two to three stages to ensure purity, and any impurities are removed using a metal mesh filter.

[0034] The resulting pure oil is used in a two-step process. In the first step, wild pistachio resin (saqez) and beeswax are added, and the mixture is heated at 65 °C for 30 minutes while being stirred with a manual mixer to ensure uniformity. The mixture is then left at room temperature for 24 hours until it is completely cooled.

[0035] In the second step, the cooled mixture is reheated to 65 °C until it is fully liquefied. Over a period of 30 minutes, castor oil, olive oil, coconut oil, turmeric, and finally honey is gradually added in sequence. The final mixture is stirred with an electric mixer for 30 minutes to ensure complete uniformity.

[0036] After mixing, the final formulation is allowed to cool at room temperature for 1 hour. It is then filled into ointment tubes to be used for animal studies.

[0037] To avoid spoilage without using preservatives, the ointment is prepared freshly each time and is stored in a refrigerator away from light.

[0038] The stages of ointment preparation in two step are presented in.Advantageous Effects of the Invention

[0039] In this study, ethical approval was obtained from the Ethics Committee on Animal Studies at Kermanshah University of Medical Sciences (Approval Code: IR.KUMS.AEC.1402.019). All procedures were conducted in full compliance with institutional guidelines for the care and use of laboratory animals, including a 12-hour light / dark cycle, a controlled temperature of 24 ± 1 °C,and55% humidity, under the supervision of an appointed university observer.

[0040] A total of 36 adult male Wistar rats (average weight: 200–250 g) were randomly divided into six groups (n = 6 per group) as follows:

[0041] Negative Control Group: Received no treatment.

[0042] Positive Control Group: Treated with 1% phenytoin cream (German formulation).

[0043] Sham Group: Treated with the ointment base (Eucerin).

[0044] Treatment Group (Ointment 0.5%): Treated with 0.5% ointment formulation.

[0045] Treatment Group (Ointment 1%): Treated with 1% ointment formulation.

[0046] Treatment Group (Ointment 1.5%): Treated with 1.5% ointment formulation.

[0047] The histopathological findings in relation to the different groups are presented in table 2.

[0048] Table 2: Histopathological findings in relation to the different groupsGroupsHistopathological interpretationNegative controlIn the negative-control group, the cutaneous architecture is not reconstituted; the epidermis and dermis are separated. Microvascular hemorrhage is observed, and angiogenesis is incomplete.Positive control (Phenytoin ointment)In the positive-control group, substantial repair is observed in the epidermal and dermal layers; however, mild inflammation and the presence of immune cells are detected. Cutaneous adnexa, including hair follicles and sweat glands, are evident, and both mature and immature blood vessels are present.Sham groupA thin and incomplete epidermis is in the process of forming a continuous epithelium, while the dermis is immature, highly vascularized, and not fully developed. Collagen fibers are in the process of formation and alignment.Ointement 0.5 %Skin tissue has formed, with regeneration and maturation of the epidermis in progress. The epidermis and dermis are integrated and unified. Numerous new blood vessels are being formed, and collagen fibers are regularly organized.Ointement 1 %The skin tissue is fully formed, with complete formation of the epidermis and full re-epithelialization. The dermis is coherently developed and integrated with the epidermis. Numerous immature hair follicles are observed in the deep dermis along with abundant neovascularization. Collagen fibers are formed and continue to develop and organize.Ointement 1.5 %Similar to the previous group, the skin tissue is fully formed, with complete formation of the epidermis and full re-epithelialization. The dermis is coherently developed and integrated with the epidermis. Numerous immature hair follicles are observed in the deep dermis, accompanied by abundant mature and immature blood vessels. Collagen fibers are formed and continue to develop and organize, indicating tissue regeneration

[0049] In this study, the rats were first anesthetized using a combination of xylazine (10 mg / kg, produced by Aflasan, Netherlands)andketamine (55 mg / kg, produced by Aflasan, Netherlands).The hair on their backs was then shaved, and standardized full-thickness excisional wounds measuring 2 cm² were created on the dorsal surface using a biopsy punch.

[0050] Following wound induction, the animals in each group received their respective treatment protocols as described previously. To prevent injury to one another, the rats were housed individually in separate cages under standard temperature conditions.

[0051] The treatment was administered twice daily for 14 days. Wound healing progression was documented photographically at different stages of treatment using a digital camera. In addition, macroscopic evaluation of wound closurewas performed bymeasuring and recording the wound area with a ruler on day 0 and day 14 for comparison

[0052] Skin and wound histology was performed by obtaining 6 mm diameter punch biopsies from the designated areas. A subset of animals from each group was euthanized 14 days after treatment using an overdose of sodium thiopental, following ethical euthanasia protocols. Skin samples were immediately collected and fixed in 10% neutral buffered formalin for 48 hours. The fixed tissue samples were then processed, embedded in paraffin, and sectioned at 5 μm thickness. Finally, the sections were stained using the hematoxylin and eosin (H&E) method. Histological slides were evaluated under a light microscope. Parameters such as epithelialization, angiogenesis, fibroplasia, and granulation tissue formation were comparatively assessed across the different groups. Both macroscopic and microscopic evaluations demonstrated that our proposed ointment formulation resulted in superior wound healing, even outperforming the commercial 1% phenytoin ointment (German formulation).Brief Description of the Drawings

[0053] Description of figures are presented below:

[0054] Two-step production process and stages for preparing 60 g of the combined animal- and plant-based ointment formulation, based on the required raw materials for (A) base formulation preparation and (B) final ointment production (from left to right, Steps 1–9).

[0055] Molded sample of the prepared ointment.

[0056] Example of an induced wound created using a sterile biopsy punch on the dorsal area of a male Wistar rat.

[0057] Study timeline: According to the experimental design, rats were divided into groups receiving no treatment, 1% phenytoin, sham (ointment base / Eucerin), and various concentrations of the ointment formulation (0.5%, 1%, and 1.5%). Treatments were administered twice daily for 14 days.

[0058] Macroscopic comparison of wound healing effects in groups receiving no treatment, 1% phenytoin, sham (Eucerin), and different concentrations of the prepared ointment formulation (0.5%, 1%, and 1.5%) on injured wound tissue from Day 1 to Day 14 in male rats.

[0059] Histological and tissue-level evaluation of negative control, phenytoin, Eucerin, and different concentrations of the prepared ointment formulation (0.5%, 1%, and 1.5%) on injured wound areas, comparing epidermis and dermis on Day 1 and Day 14 in male rats.

[0060] Pharmaceutical development and the production of medicines derived from natural sources (both plant- and animal-based) are among the industrial applications of this invention.

Claims

What is claimed is a pharmaceutical formulation in the form of an ointment, comprising sheep tail fat (15.1 g), wild pistachio resin (5 g), beeswax (5 g), castor oil (3.3 g), olive oil (3.3 g), coconut oil (3.3 g), turmeric (10 g), and honey (15 g), which, when applied topically, is effective in soothing, repairing, and treating various types of skin wounds.According to Claim 1, the formulation includes sheep tail fat, which contains polyunsaturated fatty acids with anti-inflammatory and antibiotic properties, and is used in this formulation in an amount ranging from 15.1 g to 25 g (equivalent to 41.66%–66.16%).According to Claim 1, the formulation includes wild pistachio resin (saqez), which contains polyunsaturated fatty acids and phenolic compounds providing anti-inflammatory, antioxidant, and epithelialization-promoting effects, and is used in this formulation at 3% to 5% (equivalent to 4.998 g–8.333 g).According to Claim 1, the formulation includes beeswax, which contains phenolic acids, esters, flavonoids, and other organic compounds that provide antioxidant, anti-inflammatory, antibacterial, antifungal, anti-itch, moisturizing, and emollient properties, as well as inducing fibroblast activity, promoting keratinization, and accelerating wound healing, and is used in this formulation at 3% to 5% (equivalent to 4.998 g–8.333 g).According to Claim 1, the formulation includes castor oil, which contains the active component ricinoleic acid, providing moisturizing, antioxidant, anti-inflammatory, and analgesic properties, and is used in this formulation in an amount ranging from 3.3 g to 5 g (equivalent to 5.5%–8.33%).According to Claim 1, the formulation includes olive oil, which contains the active components oleic acid and polyphenols, providing anti-inflammatory and antioxidant properties, and is used in this formulation in an amount ranging from 3.3 g to 5 g (equivalent to 5.5%–8.33%).According to Claim 1, the formulation includes coconut oil, which contains the active components lauric acid and polyphenols, providing antimicrobial, antioxidant, fibroblast proliferation–promoting, and angiogenesis-stimulating properties, and is used in this formulation in an amount ranging from 3.3 g to 5 g (equivalent to 5.5%–8.33%).According to Claim 1, the formulation includes turmeric, which contains the active component curcumin, providing antioxidant, anti-inflammatory, antibacterial properties, as well as enhancing tissue granulation, collagen synthesis, tissue regeneration, wound contraction, andaccelerating wound healing, and is used in this formulation in an amount ranging from 8 g to 10 g (equivalent to 13.328%–16.666%).According to Claim 1, the formulation includes honey, which contains water, sugars, flavonoids, organic acids, phenolic acids, vitamins, minerals, enzymes, and other compounds, providing antibacterial, hydrating, oxygenating, and wound-healing properties, and is used in this formulation in an amount ranging from 15 g to 25 g (equivalent to 25%–41.66%).According to Claim 1, the ointment production process comprises two stages: (1) preparation of the base mixture by adding sheep tail fat, wild pistachio resin, and beeswax; and (2) production of the final ointment by adding olive oil, castor oil, coconut oil, honey, and turmeric.According to Claim 10, the preparation of the ointment base formulation includes the steps of extracting sheep tail fat at 65 °C, adding wild pistachio resin and beeswax stepwise, heating the mixture for 30 minutes, and stirring the combination to achieve a uniform base mixture.According to Claim 10, the production of the final ointment formulation includes re-melting the base mixture at 65 °C, adding olive oil and castor oil stepwise while stirring, subsequently adding coconut oil and honey, then adding turmeric, stirring the final formulation for 30 minutes, and finally allowing it to cool at room temperature for 1 hour.

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