Aloe extract-based antibacterial composition and preparation method therefor

Abstract

An aloe extract-based antibacterial composition, which is a nano microemulsion and comprises the following components in percentage by mass according to soluble solids: 0.50%-10% of an aloe extract, 0.50%-5.0% of polylysine, 0.50%-20% of a caprylohydroxamic acid, 6.0%-20% of a surfactant, 15%-60% of a solvent, the remainder being water. A preparation method for the antibacterial composition comprises: mixing polylysine, an aloe extract, a caprylohydroxamic acid, a surfactant, a solvent, an optional auxiliary agent, and water, and carrying out ultrasonic treatment to prepare a uniform nano microemulsion. The aloe extract, the polylysine and the caprylohydroxamic acid are compounded by means of a microemulsion system, thereby achieving a synergistic effect, and greatly improving an antibacterial effect; and the antibacterial composition is green and safe and can serve as a bacteriostatic agent to be used in products such as daily necessities, washing and caring cosmetics and baby products.

Description

An antibacterial composition based on aloe vera extract and its preparation method Technical Field

[0001] This invention belongs to the field of antibacterial agent technology, and relates to an antibacterial agent, specifically an antibacterial composition based on aloe vera extract, and a method for preparing the composition. Background Technology

[0002] Aloe vera antibacterial technology is an innovative technology that utilizes the natural antibacterial and anti-inflammatory properties of aloe vera. The technology involves the extraction, purification, and preparation of active compounds from aloe vera. These compounds are widely believed to have inhibitory effects on various bacteria and fungi, and their applications in food, medicine, daily chemicals, cosmetics, and skincare products. The aim is to develop products with aloe vera's antibacterial properties, thereby inhibiting and killing bacteria, promoting wound healing, and effectively treating skin infections.

[0003] Aloe vera, a perennial evergreen herbaceous plant belonging to the lily family, is widely used in food, cosmetics, health products, and medicine. Aloe vera extract is a common natural product with anti-inflammatory and antibacterial properties, but its antibacterial effect is not ideal in practical applications. Aloe vera extract is often added to certain products, along with preservatives and antibacterial agents, to enhance the antibacterial effect. Due to its antibacterial effect and compatibility issues, aloe vera extract is difficult to use as a general-purpose antibacterial agent in daily necessities, toiletries, cosmetics, baby products, and household paper products, limiting its practical application as an antibacterial agent in production and daily life.

[0004] On the other hand, polylysine possesses antibacterial, antiviral, and adsorption properties, and is widely used in medical, food safety, and water treatment fields. Polylysine exhibits good broad-spectrum antibacterial properties, but its effects are primarily concentrated in the early stages of bacterial growth and require a certain amount of time to manifest. When the bacterial concentration exceeds 10⁷ CFU / mL, the inhibitory effect of polylysine on bacterial growth weakens. Furthermore, when the concentration of polylysine exceeds 1000 ppm, its antibacterial effect decreases, indicating that its antibacterial effect is concentration-limited. Simultaneously, polylysine has relatively weak antifungal activity.

[0005] Based on aloe vera extract, a preservative-free antibacterial composition with a wide range of applications and good antibacterial effect can be constructed, which can provide a green, efficient and safe antibacterial system, and provide a more comprehensive solution for the practical application and market demand of aloe vera antibacterial technology.

[0006] Summary of the Invention

[0007] The purpose of this invention is to provide a preservative-free antibacterial composition based on aloe vera extract and its preparation method. By using nanoemulsion technology to compound aloe vera extract with polylysine and octyl isohydroxamic acid, the antibacterial effect of the composition is greatly enhanced, overcoming the problems of poor antibacterial effect of aloe vera extract and weak antifungal ability of polylysine. Aloe vera extract and polylysine are natural products, and octyl isohydroxamic acid is a natural product derivative. The antibacterial system obtained by compounding these three components is more in line with the characteristics of being green, environmentally friendly, and safe.

[0008] The antibacterial composition provided by the present invention is a nanoemulsion. Aloe vera extract is compounded with polylysine and octyl isohydroxamic acid through a nanoemulsion system. The compounded nanoparticles are uniformly distributed and stable in the system and produce a synergistic effect, thereby achieving a highly efficient antibacterial effect.

[0009] To achieve the above-mentioned objectives, the present invention adopts the following technical solution:

[0010] An antibacterial composition based on aloe vera extract, characterized in that it is a nanoemulsion and comprises the following components by weight percentage:

[0011] The aloe vera extract may be in liquid or solid form. Common liquid aloe vera extracts include aloe vera gel juice and whole aloe vera leaf juice, while solid aloe vera extracts include aloe vera gel powder and whole aloe vera leaf powder. The aloe vera gel juice can be unbleached or bleached, and the whole aloe vera leaf juice can be unbleached or bleached. The aloe vera gel powder can be spray-dried or freeze-dried, and the whole aloe vera leaf powder can be spray-dried or freeze-dried. The aloe vera extract described in this invention is recommended to be a product conforming to QB / T2489-2018 or QB / T2488-2006.

[0012] The aloe extracts mentioned are preferably aloe gel spray-dried powder, whole aloe leaf spray-dried powder, aloe gel freeze-dried powder, or whole aloe leaf freeze-dried powder.

[0013] The solvent is an organic solvent with good dispersing and water-retaining capabilities, such as alcohols or esters. Alcohols include, but are not limited to, monohydric alcohols, dihydric alcohols, and polyhydric alcohols. Esters include, but are not limited to, fatty acid esters, aromatic esters, and fruit esters. Propylene glycol, glycerol, and isopropanol are preferred, along with one or more of them.

[0014] The surfactants mentioned are substances that can provide good emulsification for the system, including anionic surfactants, cationic surfactants, nonionic surfactants, biosurfactants, amino acid surfactants, peptide surfactants, or polymeric surfactants. Anionic surfactants include sulfates, sulfate esters, sulfonates, phosphate esters, phosphate esters, carboxylates, sulfosuccinates, and ethoxylated sulfates; cationic surfactants include quaternary ammonium salts, quaternary ammonium base salts, pyridinium salts, amine salts, imidazoline salts, alkylammonium salts, bisquaternary ammonium salts, and fatty amides; nonionic surfactants include ethoxylated alcohols, alkyl polysaccharides, fatty acid ethanolamides, esters, fatty alcohol polyoxyethylene ethers, fatty alcohol polyoxyethylene esters, polyoxyethylene alkylamines, polyoxyethylene alkylamides, polyethers, carbamates, and block copolymers.

[0015] Furthermore, the surfactant having an HLB value of 4-18 is preferably one or more of the following: Tween 20, Tween 40, Tween 60, Tween 80, Span 40, Span 60, Span 80, cetearyl alcohol polyether-6, cetearyl alcohol polyether-10, cetearyl alcohol polyether-15, cetearyl alcohol polyether-20, cetearyl alcohol polyether-30, cetearyl alcohol polyether-40, sodium bovine and ovine cholate, sodium cholate, and sodium deoxycholate.

[0016] The antibacterial composition is a uniform and stable nanoemulsion with an average particle size of 100-500 nm. At room temperature, it is a yellow or pale yellow translucent liquid with a pH of 3.0-8.0 and a density of 0.95 g / mL-1.2 g / mL.

[0017] Furthermore, adjuvants, including stabilizers and / or pH adjusters, may be added to the antibacterial composition.

[0018] The stabilizer is selected from esters, cyclodextrins, vitamin K, amino acids, and polymeric amino acids. Preferably, it is one or more of ethyl lactate, ethyl acetate, caprylic / capric glyceride, isopropyl laurate, vitamin K, and β-cyclodextrin.

[0019] The pH adjuster includes acids, bases, or buffers. These include, but are not limited to, inorganic acids or bases, organic acids or bases, amines, borates, amino acids and their derivatives, preferably one or more of citric acid, lactic acid, acetic acid, sodium hydroxide, potassium hydroxide, sodium carbonate, sodium bicarbonate, potassium carbonate, potassium bicarbonate, sodium dihydrogen phosphate, disodium hydrogen phosphate, sodium citrate, sodium lactate, triethanolamine, and glycine.

[0020] Preferably, the antibacterial composition comprises, by weight percentage, the following nanoemulsions:

[0021] The present invention also relates to a method for preparing the antibacterial composition. The antibacterial composition is prepared by mixing polylysine, aloe vera extract, octyl isohydroxamic acid, surfactant, solvent, adjuvant and water, followed by sonication to obtain a uniform nanoemulsion.

[0022] The stabilizers and other additives in the nano-microemulsion system of the antibacterial composition can further stabilize the prepared nano-microemulsion system, making it less prone to demulsification, layering, and coarsening, thus ensuring the uniformity of the nano-microemulsion system. This further enables the compounded product to be more uniformly dispersed in the aqueous phase, allowing it to dissolve in water more quickly and effectively in applications, thereby significantly improving the antibacterial effect of the antibacterial composition.

[0023] Beneficial effects: The antibacterial composition of this invention is a uniform and stable nanoemulsion. Through the microemulsion system, aloe vera extract, polylysine, and octyl isohydroxamic acid are compounded, achieving a synergistic effect and significantly improving the antibacterial efficacy, not only at a concentration of 10... 9 It exhibits an inhibitory effect on bacteria at a concentration of CFU / mL and also shows good inhibitory effects on fungi. The antibacterial composition is a yellow or pale yellow translucent liquid at room temperature, with a pH of 3.0-8.0, a density of 0.95 kg / L-1.2 kg / L, and an average particle size of 100-500 nm. It is fluid at room temperature (20-25℃). After repeated freeze-thaw cycles at low temperatures, the compound product maintains a uniform particle size distribution. After repeated heating, the compound product also maintains a uniform particle size distribution, and temperature does not affect its nanoemulsion structure. The compound product is completely soluble in water and can be well dispersed in water, emulsions, serums, and other systems, making it suitable for various applications.

[0024] The antibacterial composition is mainly used in daily necessities, toiletries, cosmetics, baby products and household paper products as an antibacterial agent. Attached Figure Description

[0025] Figure 1 shows the color and morphology of the antibacterial compositions prepared in Example 1 (left) and Example 2 (right);

[0026] Figure 2. Particle size distribution of the antibacterial composition prepared in Example 1;

[0027] Figure 3. Photograph of the aqueous solution of the antibacterial composition prepared in Example 1;

[0028] Figure 4. Determination of the minimum inhibitory concentration of the antibacterial composition prepared in Example 1 against Escherichia coli;

[0029] Figure 5. The antibacterial effects of aloe vera extract (left), antibacterial composition (middle), and polylysine (right) on Staphylococcus aureus;

[0030] Figure 6. The antibacterial effects of aloe vera extract (left), antibacterial composition (middle), and polylysine (right) on Escherichia coli;

[0031] Figure 7. The antibacterial effects of aloe vera extract (left), antibacterial composition (middle), and polylysine (right) on Candida albicans;

[0032] Figure 8. The antibacterial effects of aloe vera extract (left), antibacterial composition (middle), and polylysine (right) on Aspergillus niger;

[0033] Figure 9 shows the long-term antibacterial results of the antibacterial composition added to the face cream. The left photo is the initial photo of the face cream, and the right photo is the photo of the face cream after 5 months.

[0034] Figure 10 shows the color and morphology of the compositions prepared in Comparative Example 1 (left) and Comparative Example 2 (right). Detailed Implementation

[0035] The technical solution of the present invention will be further described below through specific embodiments. However, it should be noted that the following embodiments are only used to describe the content of the present invention and do not constitute a limitation on the scope of protection of the present invention.

[0036] Example 1

[0037] An antibacterial composition based on aloe vera extract is a uniform and stable nanoemulsion comprising, by weight percentage: 44% propylene glycol, 15% cetearyl alcohol polyether-20, 1.5% freeze-dried whole aloe vera leaf powder, 16% octyl isohydroxamic acid, 2.0% polylysine, 8.5% ethyl lactate, with the balance being water, adjusted to pH 3.9.

[0038] The method for preparing the antibacterial composition involves adding polylysine, freeze-dried whole aloe vera leaf powder, octyl isohydroxamic acid, surfactant cetearyl alcohol polyether-20, propylene glycol, ethyl lactate, and water to a container, adjusting the pH to 3.9, and sonicating to fully mix until a uniform nanoemulsion is obtained.

[0039] The antibacterial composition prepared above is stable, as shown in Figure 1. At room temperature (20-25℃), it is a yellow, semi-transparent liquid with a density of 1.05 kg / L. Its particle size was measured, as shown in Figure 2, with an average particle size of 376 nm. At a wavelength of 570 nm, the transmittance of a 2 mm thick cuvette chamber was 68%.

[0040] The above antibacterial composition was added to water at different proportions, such as 0.05%, 0.50%, and 1.5%, and the solution remained clear and transparent after addition. The aqueous solution of the antibacterial composition is shown in Figure 3. After repeated freeze-thaw cycles and repeated heating, the above nanoemulsion did not exhibit demulsification, stratification, or coarsening; it maintained a uniform particle size distribution and a stable state, and its antibacterial activity was unaffected.

[0041] Antibacterial effect

[0042] The bacterial strains selected for the experiment, referring to preservative challenge tests (such as CTFA / USP / China Cosmetic Testing Standards), were as follows: *Escherichia coli* ATCC 8739, *Pseudomonas aeruginosa* ATCC 9027, *Acinetobacter baumannii* ATCC 19606, *Bacillus subtilis* ATCC 6051, *Staphylococcus aureus* ATCC 6538, *Klebsiella pneumoniae* ATCC 4352, *Salmonella enterica* ATCC 14028, *Candida albicans* ATCC 10231, and *Aspergillus niger* ATCC 16404. The minimum inhibitory concentration (MIC) of the antimicrobial composition against different microorganisms was tested using the disk diffusion method. Taking *Escherichia coli* as an example, the antimicrobial effect of the 0.50 g / L–5.0 g / L antimicrobial composition against *Escherichia coli* and other bacteria is shown in Figure 4.

[0043] The MIC determination results of the antibacterial composition against different bacterial species are shown in Table 1.

[0044] Table 1. Minimum inhibitory concentrations of the antibacterial compositions against different bacterial species.

[0045] The inhibitory effects of this antibacterial composition compared with those of aloe vera extract or polylysine alone at the same concentration were shown in Figures 5-8, with results against Staphylococcus aureus (Gram-positive), Escherichia coli (Gram-negative), Candida albicans (fungus), and Aspergillus niger (mold). It is evident that the antibacterial composition exhibits significantly higher inhibitory activity against all tested Gram-positive, Gram-negative, fungal, and mold species than aloe vera extract and polylysine alone. This indicates that the combination of aloe vera extract, polylysine, and octylhydroxamic acid in the microemulsion system significantly improves the shortcomings in antibacterial performance.

[0046] The antibacterial composition was added to both a light-textured and a thick-textured face cream to test its antibacterial effect. The results are shown in Figure 9. The antibacterial composition was added at a concentration of 0.50% to the face cream. The creams were left at room temperature for 5 months, during which time the lids were repeatedly opened to simulate usage scenarios. Ultimately, no bacterial growth was observed in either type of face cream, and the addition of the antibacterial composition did not disrupt the existing emulsification system of the face creams.

[0047] Example 2

[0048] The preparation method is consistent with that of Example 1, and its components include: 37% propylene glycol, 1.5% Span 80, 12% Tween 80, 0.50% freeze-dried whole aloe vera leaf powder, 16% octyl isohydroxamic acid, 2.0% polylysine, 15% ethyl lactate, and the balance being water, adjusted to pH 7.8.

[0049] The physicochemical properties of the antibacterial composition prepared above are basically the same as those of Example 1 (Figure 1), with an average particle size of 385 nm and a light transmittance of 69%.

[0050] The antibacterial effect of the antimicrobial composition was tested using the same method as in Example 1. The minimum inhibitory concentrations (MICs) of the antimicrobial composition against different microorganisms were basically consistent with those in Table 1.

[0051] Example 3

[0052] The preparation method is consistent with that of Example 1, and its components include: 37% propylene glycol, 0.60% Span 80, 6.0% Tween 80, 6.7% Tween 20, 2.6% freeze-dried aloe vera gel powder, 16% octyl isohydroxamic acid, 2.0% polylysine, 15% ethyl lactate, and the balance being water, adjusted to pH 7.0.

[0053] The physicochemical properties of the antibacterial composition prepared above are basically the same as those in Example 1, with an average particle size of 320 nm and a light transmittance of 65%.

[0054] The antibacterial test method was the same as in Example 1. The MIC of the antibacterial composition is shown in Table 2.

[0055] Table 2 shows the minimum inhibitory concentrations of the composition in Example 3 against different bacterial species.

[0056] Example 4

[0057] The preparation method is consistent with that of Example 1, and its components include: 38% propylene glycol, 0.60% Span 80, 6.0% Tween 80, 6.7% Tween 20, 0.70% freeze-dried aloe vera gel powder, 8.0% octyl isohydroxamic acid, 3.0% polylysine, 15% ethyl lactate, and the balance being water, with the pH adjusted to 6.5.

[0058] The physicochemical properties of the antibacterial composition prepared above are basically the same as those in Example 1, with an average particle size of 417 nm and a light transmittance of 66%.

[0059] The antibacterial test method was the same as in Example 1. The MIC of the antibacterial composition is shown in Table 3.

[0060] Table 3. Minimum inhibitory concentrations of the composition in Example 4 against different bacterial species.

[0061] Example 5

[0062] The preparation method is consistent with that of Example 1, and its components include: 16% propylene glycol, 1.8% Span 80, 18% Tween 80, 1.0% freeze-dried whole aloe vera leaf powder, 1.5% octylhydroxamic acid, 2.0% polylysine, with the balance being water, and the pH adjusted to 6.5.

[0063] The physicochemical properties of the antibacterial composition prepared above are basically the same as those in Example 1, with an average particle size of 296 nm and a light transmittance of 63%.

[0064] The antibacterial test method was the same as in Example 1. The MIC of the antibacterial composition is shown in Table 4.

[0065] Table 4 shows the minimum inhibitory concentrations of the composition in Example 5 against different bacterial species.

[0066] Example 6

[0067] The preparation method is consistent with that of Example 1, and its components include: 44% propylene glycol, 10% cetearyl alcohol polyether-6, 1.2% aloe vera gel freeze-dried powder, 16% octyl isohydroxamic acid, 2.0% polylysine, 11% ethyl lactate, and the balance being water, with the pH adjusted to 4.5.

[0068] The physicochemical properties of the antibacterial composition prepared above are similar to those of Example 1, and the antibacterial effect is consistent with that of Example 1. Its average particle size is 293 nm and its light transmittance is 62%.

[0069] Example 7

[0070] The preparation method is consistent with that of Example 1, and its components include: 38% glycerol, 1.2% Span 60, 12% Tween 80, 0.80% freeze-dried whole aloe vera leaf powder, 16% octyl hydroxamic acid, 2.0% polylysine, 16% ethyl lactate, and the balance being water, adjusted to pH 3.9.

[0071] The physicochemical properties of the antibacterial composition prepared above are similar to those of Example 1, and the antibacterial effect is consistent with that of Example 1. Its average particle size is 219 nm and its light transmittance is 63%.

[0072] Example 8

[0073] The preparation method is consistent with that of Example 1, and its components include: 38% glycerol, 15% cetearyl alcohol polyether-20, 0.70% aloe vera gel freeze-dried powder, 12% octyl isohydroxamic acid, 2.0% polylysine, 15% ethyl lactate, and the balance being water, adjusted to pH 6.0.

[0074] The physicochemical properties of the antibacterial composition prepared above are similar to those of Example 1, and the antibacterial effect is consistent with that of Example 3. Its average particle size is 313 nm and its light transmittance is 62%.

[0075] Example 9

[0076] The preparation method is consistent with that of Example 1, and its components include: 44% propylene glycol, 10% cetearyl alcohol polyether-40, 1.2% whole aloe vera leaf juice, 10% octyl hydroxamic acid, 2.0% polylysine, 11% caprylic / capric glyceride, and the balance being water, with the pH adjusted to 3.9.

[0077] The physicochemical properties of the antibacterial composition prepared above are similar to those of Example 1, and the antibacterial effect is consistent with that of Example 3. Its average particle size is 211 nm and its light transmittance is 60%.

[0078] Example 10

[0079] The preparation method is consistent with that of Example 1, and its components include: 38% propylene glycol, 1.2% Span 80, 12% Tween 80, 0.90% freeze-dried whole aloe vera leaf powder, 16% octyl isohydroxamic acid, 3.0% polylysine, 12% ethyl acetate, and the balance being water, adjusted to pH 6.5.

[0080] The physicochemical properties of the antibacterial composition prepared above are similar to those of Example 1, and the antibacterial effect is consistent with that of Example 3. Its average particle size is 334 nm and its light transmittance is 66%.

[0081] Example 11

[0082] The preparation method is the same as in Example 1, and its components include: 38% glycerol, 0.60% Span 80, 6.0% Tween 80, 6.8% Tween 20, 0.70% (soluble solids) aloe vera gel juice, 8.0% octyl hydroxamic acid, 2.0% polylysine, 15% isopropyl laurate, and the balance is water, adjusted to pH 4.5.

[0083] The physicochemical properties of the antibacterial composition prepared above are similar to those of Example 1, and the antibacterial effect is consistent with that of Example 1. Its average particle size is 288 nm and its light transmittance is 68%.

[0084] Example 12

[0085] The preparation method is the same as in Example 1, and its components include: 38% isopropanol, 1.2% Span 80, 12% Tween 80, 0.50% (soluble solids) whole aloe vera leaf juice, 16% octylhydroxamic acid, 2.0% polylysine, 15% ethyl lactate, and the balance being water, with the pH adjusted to 7.8.

[0086] The physicochemical properties of the antibacterial composition prepared above are similar to those of Example 1, and the antibacterial effect is consistent with that of Example 1. Its average particle size is 267 nm and its light transmittance is 67%.

[0087] Example 13

[0088] The preparation method is the same as in Example 1. Its components include: 38% propylene glycol, 1.5% Span 60, 14% Tween 80, 0.50% freeze-dried whole aloe vera leaf powder, 16% octylhydroxamic acid, 2.0% polylysine, 15% caprylic / capric triglyceride, and the balance being water, with the pH adjusted to 6.8.

[0089] The physicochemical properties of the antibacterial composition prepared above are similar to those of Example 1, and the antibacterial effect is consistent with that of Example 1. Its average particle size is 311 nm and its light transmittance is 67%.

[0090] Example 14

[0091] The preparation method is the same as in Example 1. Its components include: 38% propylene glycol, 1.5% Span 80, 6.5% Tween 80, 6.5% Tween 20, 1.5% freeze-dried whole aloe vera leaf powder, 16% octyl isohydroxamic acid, 2.0% polylysine, 13% vitamin K, and the balance being water, with the pH adjusted to 4.0.

[0092] The physicochemical properties of the antibacterial composition prepared above are similar to those of Example 1, and the antibacterial effect is consistent with that of Example 1. Its average particle size is 446 nm and its light transmittance is 69%.

[0093] Example 15

[0094] The preparation method is the same as in Example 1. Its components include: 38% propylene glycol, 1.5% Span 80, 12% Tween 80, 0.70% aloe vera gel freeze-dried powder, 16% octyl isohydroxamic acid, 2.0% polylysine, 15% ethyl lactate, and the balance is water, with the pH adjusted to 7.0.

[0095] The physicochemical properties of the antibacterial composition prepared above are similar to those of Example 1, and the antibacterial effect is consistent with that of Example 1. Its average particle size is 328 nm and its light transmittance is 63%.

[0096] Example 16

[0097] An antibacterial composition comprising: 38% propylene glycol, 12% cetearyl alcohol polyether-6, 0.50% freeze-dried whole aloe vera leaf powder, 16% octyl isohydroxamic acid, 2.0% polylysine, 15% ethyl lactate, with the balance being water, and the pH adjusted to 4.5.

[0098] The nanoemulsion prepared above has an average particle size of 412 nm and a light transmittance of 67%. The physicochemical properties of the antibacterial composition are the same as those in Example 1, and the antibacterial effect is consistent with that in Example 3.

[0099] Example 17

[0100] An antibacterial composition comprising: 45% propylene glycol, 1.5% Span 60, 12% Tween 80, 0.80% (soluble solids) aloe vera gel juice, 16% octyl isohydroxamic acid, 2.0% polylysine, 15% ethyl lactate, with the balance being water, adjusted to pH 7.5.

[0101] The nanoemulsion prepared above has an average particle size of 402 nm and a light transmittance of 65%. The physicochemical properties of the antibacterial composition are the same as those in Example 1, and the antibacterial effect is consistent with that in Example 1.

[0102] Example 18

[0103] An antibacterial composition comprising: 38% propylene glycol, 1.5% Span 80, 12% Tween 60, 1.5% Tween 20, 0.50% (soluble solids) whole aloe vera leaf juice, 16% octylhydroxamic acid, 2.0% polylysine, 8.0% ethyl acetate, 7.0% ethyl lactate, with the balance being water, adjusted to pH 4.5.

[0104] The nanoemulsion prepared above has an average particle size of 395 nm and a light transmittance of 62%. The physicochemical properties of the antibacterial composition are the same as those in Example 1, and the antibacterial effect is consistent with that in Example 1.

[0105] Example 19

[0106] An antibacterial composition comprising: 46% propylene glycol, 12% cetearyl alcohol polyether-40, 1.5% Tween 20, 4.0% freeze-dried whole aloe vera leaf powder, 8.0% octyl isohydroxamic acid, 2.0% polylysine, 15% ethyl lactate, with the balance being water, adjusted to pH 6.8.

[0107] The nanoemulsion prepared above has an average particle size of 329 nm and a light transmittance of 66%. The physicochemical properties of the antibacterial composition are the same as those in Example 1, and the antibacterial effect is consistent with that in Example 3.

[0108] Example 20

[0109] An antibacterial composition comprising: 38% propylene glycol, 1.5% Span 80, 12% Tween 80, 1.5% Tween 20, 0.50% aloe vera gel juice, 16% octylhydroxamic acid, 2.0% polylysine, 7.0% caprylic / capric triglyceride, 7.0% ethyl lactate, with the balance being water, and the pH adjusted to 6.0.

[0110] The nanoemulsion prepared above has an average particle size of 304 nm and a light transmittance of 70%. The physicochemical properties of the antibacterial composition are the same as those in Example 1, and the antibacterial effect is consistent with that in Example 1.

[0111] Comparative Example 1

[0112] The preparation method is consistent with that in Example 1, and its components include: 44% propylene glycol, 5.0% Tween 80, 0.50% Span 80, 1.2% freeze-dried whole aloe vera leaf powder, 16% octyl isohydroxamic acid, 2.0% polylysine, 16% ethyl lactate, and the balance being water.

[0113] The above composition cannot form a uniform and stable nanoemulsion system. As shown in Figure 9, a large number of crystals precipitate out after standing.

[0114] Comparative Example 2

[0115] The preparation method is consistent with that in Example 1, and its components include: 59% propylene glycol, 6.0% Tween 80, 0.60% Span 80, 1.2% (soluble solids) aloe vera gel juice, 22% octyl isohydroxamic acid, 2.0% polylysine, and the balance being water.

[0116] The above composition cannot form a uniform and stable nanoemulsion system. As shown in Figure 10, a large number of crystals precipitate out after standing.

[0117] Finally, it should be noted that the above descriptions are merely preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions described in the foregoing embodiments or make equivalent substitutions for some of the technical features. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the protection scope of the present invention.

Claims

1. An antibacterial composition based on aloe vera extract, characterized in that, The microemulsion is composed of the following components by mass percentage:

2. The antibacterial composition based on aloe vera extract according to claim 1, characterized in that, The aloe extract is selected from aloe vera gel juice, whole aloe vera leaf juice, aloe vera gel powder, or whole aloe vera leaf powder.

3. The antibacterial composition based on aloe vera extract according to claim 1, characterized in that, The solvent is selected from one or more of propylene glycol, glycerol, and isopropanol.

4. The antibacterial composition based on aloe vera extract according to claim 1, characterized in that, The surfactant has an HLB value of 4-18.

5. The antibacterial composition based on aloe vera extract according to claim 4, characterized in that, The surfactant is selected from one or more of Tween 20, Tween 40, Tween 60, Tween 80, Span 40, Span 60, Span 80, cetearyl alcohol polyether-6, cetearyl alcohol polyether-10, cetearyl alcohol polyether-15, cetearyl alcohol polyether-20, cetearyl alcohol polyether-30, cetearyl alcohol polyether-40, sodium bovine and ovine cholate, sodium cholate, and sodium deoxycholate.

6. The antibacterial composition based on aloe vera extract according to claim 4, characterized in that, The nanoemulsion has an average particle size of 100-500 nm, a pH of 3.0-8.0, and a density of 0.95 g / mL-1.2 g / mL.

7. The antibacterial composition based on aloe vera extract according to claim 1, characterized in that, The antibacterial composition further includes additives selected from stabilizers and / or pH adjusters.

8. The antibacterial composition based on aloe vera extract according to claim 7, characterized in that, The stabilizer is selected from one or more of ethyl lactate, ethyl acetate, caprylic / capric triglyceride, isopropyl laurate, vitamin K, and β-cyclodextrin.

9. The antibacterial composition based on aloe vera extract according to claim 1, characterized in that, The nanoemulsion, by mass percentage, consists of the following components:

10. A method for preparing the antibacterial composition based on aloe vera extract according to any one of claims 1 to 9, characterized in that, Polylysine, aloe vera extract, octyl isohydroxamic acid, surfactant, solvent, additives and water were mixed and sonicated to obtain a uniform nanoemulsion.

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