Cosmetic composition comprising fermented oat sprout extract, and preparation method therefor

Abstract

The present invention relates to a cosmetic composition comprising a fermented oat sprout extract, and a preparation method therefor, the cosmetic composition comprising a fermented oat sprout extract obtained by fermenting an oat sprout extract obtained by extracting oats sprouts, thereby having various effects on the skin.

Description

Cosmetic composition containing fermented sprout oat extract and method for producing the same

[0001] The present invention relates to a cosmetic composition comprising a fermented extract of oat sprouts and a method for producing the same, and more particularly, to a cosmetic composition comprising a fermented extract of oat sprouts obtained by fermenting an oat sprout extract extracted from oat sprouts and having various effects on the skin, and a method for producing the same.

[0002] Oat leaves have long been used as medicine and are known to be rich in nutrients, including vitamins, enzymes, and chlorophyll. Recently, the excellent nutrients and health functional substances contained in oats have become known for their effectiveness in balancing diets and improving physical constitution. This has led to increased interest in oats as a functional raw material for health and well-being. They are also receiving excellent evaluations as livestock feed.

[0003] Sprouted oats are young plants that have sprouted and grown to about 15 cm. Generally, oats are soaked in water for about a day, drained, and sown in a plastic basket or Styrofoam of an appropriate size lined with newspaper, covered with paper until they sprout, and then removed once they sprout and kept moist for about 7-10 days until they are 15 cm tall. It is known that oat sprouts contain a significantly higher content of dietary fiber, carotene, and vitamin C than other vegetables and fruits. In addition, in terms of pharmacological activity, oat sprouts are known to have the effects of lowering cholesterol, preventing constipation, preventing arteriosclerosis, having antioxidant activity, preventing aging, preventing osteoporosis, improving insomnia, and preventing anemia.

[0004] Various studies are being conducted on cosmetic compositions utilizing sprouted oats. Republic of Korea Patent Publication No. 10-2023-0127504 (published on September 1, 2023) discloses, "Sprouted oat postbiotic composition with skin whitening and wrinkle-improving activity and use thereof." This composition contains a fermented sprouted oat (Avena sativa) product, which is designed to have whitening and wrinkle-improving effects. However, the combination of sprouted oats and the Lactobacillus plantarum, Lactobacillus rhamnosus, Lactobacillus casei and Lactobacillus gasseri strains of the conventional patented technology did not show any remarkable effect, and accordingly, research is actively being conducted on a composition with improved skin and health effects by grafting other strains onto sprouted oats and fermenting them.

[0005] [Prior Art Literature]

[0006] [Patent Document]

[0007] (Patent Document 0001) Republic of Korea Patent Publication No. 10-2023-0127504 (published on September 1, 2023), "Sprouted oat postbiotics composition having skin whitening and wrinkle improvement activity and use thereof"

[0008] In order to solve the above problems, the present invention relates to a cosmetic composition including a fermented extract of oat sprouts and a method for producing the same, and more specifically, to a cosmetic composition including a fermented extract of oat sprouts obtained by fermenting an oat sprout extract extracted from oat sprouts and having various effects on the skin, and a method for producing the same.

[0009] In order to achieve the above purpose, the present invention provides a cosmetic composition comprising a fermented oat sprout extract, characterized in that it comprises a fermented oat sprout extract obtained by fermenting a sprout extract obtained by extracting sprout oats.

[0010] At this time, the above sprouted oat extract is characterized by being obtained by mixing sprouted oats with purified water at a weight ratio of 1:20, extracting at a temperature of 80 to 100°C for 6 to 10 hours, and filtering.

[0011] At this time, the above-mentioned sprouted oat extract fermentation product is characterized by being obtained by fermenting sprouted oat extract using Galactomyces candidus strain at a temperature of 25 to 40°C for 12 to 50 hours and filtering.

[0012] At this time, the above sprouted oat extract fermentation is characterized by being concentrated using a reduced pressure concentrator to 50 to 90 brix.

[0013] In addition, the present invention provides a method for producing a cosmetic composition including a fermented oat sprout extract, characterized in that it comprises a step (S10) of obtaining a sprout extract by extracting sprout oats; a step (S20) of obtaining a fermented oat sprout extract by fermenting the sprout oat extract; and a step (S30) of concentrating the fermented oat sprout extract using a reduced pressure concentrator.

[0014] The cosmetic composition comprising a fermented oat sprout extract according to the manufacturing method of the present invention relates to a cosmetic composition comprising a fermented oat sprout extract and a manufacturing method thereof, and more specifically, to a fermented oat sprout extract obtained by fermenting oat sprout extract, thereby providing various effects on the skin.

[0015] Figure 1 is a graph regarding the cytotoxicity confirmation experiment of Example 1 and Comparative Example 1 according to the present invention.

[0016] Figure 2 is a graph regarding the moisturizing effect confirmation experiment of Example 1 and Comparative Example 1 according to the present invention.

[0017] Figure 3 is a graph regarding the Astringent confirmation experiment of Example 1 and Comparative Example 1 according to the present invention.

[0018] Figure 4 is a graph regarding an experiment confirming the elastase inhibition effect of Example 1 and Comparative Example 1 according to the present invention.

[0019] Figure 5 is a graph regarding an experiment to confirm the collagenase inhibition effect of Example 1 and Comparative Example 1 according to the present invention.

[0020] Figure 6 is a graph regarding an experiment to confirm the antioxidant effect of Example 1 and Comparative Example 1 according to the present invention.

[0021] Figure 7 is an image of a cell binding force confirmation experiment of Example 1 and Comparative Example 1 according to the present invention.

[0022] Figure 8 is a graph showing the increase rate of Claudin-1 during the cell binding force confirmation experiment of Example 1 and Comparative Example 1 according to the present invention.

[0023] Figure 9 is a graph regarding the fat differentiation inhibition activity of Example 1 and Comparative Example 1 according to the present invention.

[0024] Figure 10 is a graph showing the increase rate of Desmoglein 2 during the cell binding force confirmation experiment of Example 1 and Comparative Example 1 according to the present invention.

[0025] Figure 11 is an image of the fat differentiation inhibition activity of Example 1 and Comparative Example 1 according to the present invention.

[0026] Figure 12 is an image of a sebum control-biomarker confirmation experiment of Example 1 and Comparative Example 1 according to the present invention.

[0027] Figure 13 is a graph regarding the PPAR γ expression level during the sebum control-biomarker confirmation experiment of Example 1 and Comparative Example 1 according to the present invention.

[0028] Figure 14 is a graph regarding the expression level of C / EBP α during the sebum control-biomarker confirmation experiment of Example 1 and Comparative Example 1 according to the present invention.

[0029] Figure 15 is a graph regarding a simple clinical moisturizing experiment of Comparative Example 2, Manufacturing Example 1, Comparative Example 3, and Comparative Example 4 according to the present invention.

[0030] The following detailed description of the present invention provides examples of embodiments in which the present invention may be practiced, and reference is made to the accompanying drawings, which illustrate examples of such embodiments. These embodiments are described in sufficient detail to enable those skilled in the art to practice the present invention. It should be understood that the various embodiments of the present invention, while different from each other, are not necessarily mutually exclusive. For example, specific shapes, structures, and characteristics described herein may be implemented in other embodiments without departing from the spirit and scope of the present invention. Furthermore, it should be understood that the positions or arrangements of individual components within each described embodiment may be changed without departing from the spirit and scope of the present invention.

[0031] Accordingly, the detailed description set forth below is not intended to be limiting, and the scope of the present invention is defined solely by the appended claims, along with the full scope equivalent to what such claims, if properly described, would encompass. Similar reference numerals in the drawings designate the same or similar features throughout.

[0032] The terms used in this invention have been selected from widely used, current terms, taking into account the functions of the invention. However, these terms may vary depending on the intentions of those skilled in the art, precedents, the emergence of new technologies, etc. Furthermore, in certain cases, terms may be arbitrarily selected by the applicant, in which case their meanings will be described in detail in the relevant description of the invention. Therefore, the terms used in this invention should not be defined simply as names, but rather based on their inherent meanings and the overall content of the invention.

[0033] When it is said in the present invention that a part “includes” a certain component, this does not mean that other components are excluded, but rather that other components may be included, unless otherwise specifically stated.

[0034]

[0035] Hereinafter, a cosmetic composition containing a fermented sprout extract according to the present invention will be described in detail.

[0036]

[0037] A cosmetic composition comprising a fermented oat sprout extract according to the present invention comprises a fermented oat sprout extract obtained by extracting oat sprouts and a fermented oat sprout extract obtained by fermenting the same.

[0038]

[0039] The above-mentioned sprouted oats refer to young shoots of oats (Avena sativa L.) less than 20 cm long that have sprouted and grown. The above-mentioned oats are a biennial herb of the gramineae family, native to Central Asia, and have been cultivated in Korea since the Goryeo Dynasty. They grow in clumps at the base and can reach a height of 1 m. The above-mentioned oat stems are straight and almost hairless, but the nodes have downward-facing hairs. The above-mentioned oats are a starchy edible plant and are widely cultivated in temperate regions. Although oats are mainly used as livestock feed, they are also peeled, pressed flat, and eaten to make oatmeal or cookies.

[0040]

[0041] The above sprouted oat extract can be obtained by mixing sprouted oats with purified water at a weight ratio of 1:20, extracting at a temperature of 80 to 100°C for 6 to 10 hours, and filtering.

[0042] More specifically, the above sprouted oat extract is preferably obtained by mixing sprouted oats with purified water at a weight ratio of 1:20, extracting at a temperature of 90°C for 8 hours, and filtering.

[0043]

[0044] The above-mentioned sprouted oat extract fermentation product can be obtained by fermenting sprouted oat extract using Galactomyces candidus strain at a temperature of 25 to 40°C for 12 to 50 hours and filtering.

[0045] More specifically, the above-mentioned sprouted oat extract fermentation product is preferably obtained by fermenting sprouted oat extract using a Galactomyces candidus strain at a temperature of 30 to 35°C for 24 to 48 hours and filtering with a 0.2 μm filter paper.

[0046] The above Galactomyces candidus strain is a commercially available strain, has a biosafety rating of Level 1 by the U.S. Centers for Disease Control and Prevention, and is a type of yeast strain involved in the fermentation of cheese.

[0047]

[0048] The above-mentioned sprouted oat extract fermentation product can be concentrated using a vacuum concentrator to 50 to 90 brix. More specifically, it is preferable to concentrate the above-mentioned sprouted oat extract fermentation product using a vacuum concentrator to 70 brix.

[0049]

[0050] Meanwhile, the present invention provides a cosmetic composition characterized in that it is manufactured in the form of a toner, skin, lotion, cream, foundation, essence, gel, pack, emulsified UV protection cream, emulsified foundation, emulsified makeup base, oil cake-type foundation, two-way cake, or powder pact, including the fermented sprout extract of the above.

[0051] The present invention includes, in addition to the fermented sprout extract of the present invention, ingredients commonly used in cosmetic compositions, such as conventional auxiliary agents such as antioxidants, stabilizers, solubilizers, vitamins, pigments, coloring agents and fragrances, and carriers.

[0052] The cosmetic composition of the present invention can be manufactured into any formulation commonly manufactured in the art, and for example, can be formulated into a toner, skin, lotion, cream, foundation, essence, gel, pack, emulsified UV protection cream, emulsified foundation, emulsified makeup base, oil cake type foundation, two-way cake, or powder pact, but is not limited thereto. More specifically, it can be manufactured into a formulation of a flexible toner, a nourishing toner, a nourishing cream, a massage cream, an essence, a pack, an eye cream, a cleansing cream, a cleansing foam, cleansing water, a pack, a spray, or a powder.

[0053] When the formulation of the present invention is a paste, cream or gel, one or more of animal oil, vegetable oil, wax, paraffin, starch, tragacanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be selected and used as a carrier component.

[0054] When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and particularly in the case of a spray, a propellant such as chlorofluorohydrocarbon, propane / butane or dimethyl ether may be additionally included.

[0055] When the formulation of the present invention is a solution or emulsion, a solvent, a solubilizer or an emulsifier is used as a carrier component, for example, water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, butylene glycol, 1,3-butyl glycol oil, polyoxyethylene hydrogenated castor oil, glycerol, glycerin, aliphatic ester, phenoxyethanol, triethanolamine, polyethylene glycol, beeswax, polysorbate 60, sorbitan sesquioleate, paraffin, sorbitan stearate, lipophilic monostearate glycerin, stearic acid, glyceryl stearate / PEG-400 stearate, carboxypolymer, sitosterol, polyglyceryl 2-oleate, ceramide, cholesterol, steareth-4, dicetyl phosphate, macadamia oil, Any one or more of carboxyvinyl polymer, xanthan gum or sorbitan fatty acid esters may be selected and used.

[0056] When the formulation of the present invention is a suspension, one or more of a liquid diluent such as water, ethanol, glycerin, butylene glycol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline cellulose, hydroxyethyl cellulose, sodium hyaluronate, phenoxyethanol, aluminum metahydroxide, bentonite, stearic acid, cetyl alcohol, glyceryl monostearate, polyoxyethylene sorbitan monostearate, sorbitan sesquioleate, glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate, wax, paraffin, squalane, caprylic / capric triglyceride, carboxyvinyl polymer, triethanolamine, agar or tragacanth may be selected and used as a carrier component.

[0057] When the formulation of the present invention is a surfactant-containing cleansing agent, any one or more of aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, isethionate, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkylamidobetaine, fatty alcohol, fatty acid glyceride, fatty acid diethanolamide, vegetable oil, lanolin derivative, or ethoxylated glycerol fatty acid ester may be selected and used as a carrier component.

[0058]

[0059] In addition, the present invention provides a method for producing a cosmetic composition including a fermented oat sprout extract, characterized in that it comprises a step (S10) of obtaining a sprout extract by extracting sprout oats; a step (S20) of obtaining a fermented oat sprout extract by fermenting the sprout oats extract; and a step (S30) of concentrating the fermented oat sprout extract using a vacuum concentrator.

[0060]

[0061] In the step of obtaining the oat sprout extract (S10), oat sprouts are extracted to obtain an oat sprout extract. In the step of obtaining the oat sprout extract (S10), the oat sprout extract can be obtained by mixing oat sprouts and purified water in a weight ratio of 1:20, extracting at a temperature of 80 to 100°C for 6 to 10 hours, and filtering. More specifically, in the step of obtaining the oat sprout extract (S10), it is preferable to obtain an oat sprout extract by mixing oat sprouts and purified water in a weight ratio of 1:20, extracting at a temperature of 90°C for 8 hours, and filtering.

[0062] In the step of obtaining the fermented oat extract (S20), the fermented oat extract is fermented to obtain the fermented oat extract. In the step of obtaining the fermented oat extract (S20), the fermented oat extract can be obtained by fermenting the fermented oat extract using a Galactomyces candidus strain for 12 to 50 hours at a temperature of 25 to 40°C and filtering the fermented oat extract. More specifically, in the step of obtaining the fermented oat extract (S20), it is preferable to ferment the fermented oat extract using a Galactomyces candidus strain for 24 to 48 hours at a temperature of 30 to 35°C and filtering the fermented oat extract with a 0.2 μm filter paper to obtain the fermented oat extract. Meanwhile, in the step of obtaining the above-mentioned sprout fermented extract (S20), filtration may be performed and then a sterilization process may be performed.

[0063] In the above concentration step (S30), the sprouted oat extract fermentation is concentrated using a vacuum concentrator. In the above concentration step (S30), the sprouted oat extract fermentation can be concentrated using a vacuum concentrator to a brix of 50 to 90. More specifically, in the above concentration step (S30), it is preferable to concentrate the sprouted oat extract fermentation using a vacuum concentrator to a brix of 70.

[0064]

[0065] Hereinafter, the effects of a cosmetic composition containing a fermented sprout oat extract according to the present invention will be examined in detail through the following examples, comparative examples, and experimental examples.

[0066]

[0067] Example 1. Preparation of fermented oat sprout extract

[0068] Samples for the experiment were prepared according to the following process.

[0069] Step for obtaining sprouted oat extract (S10): Sprouted oats were mixed with purified water at a weight ratio of 1:20, extracted at a temperature of 90°C for 8 hours, and filtered to obtain a sprouted oat extract.

[0070] Step for obtaining fermented oat sprout extract (S20): The above fermented oat sprout extract was fermented using a Galactomyces candidus strain at a temperature of 30 to 35°C for 24 to 48 hours, and filtered through a 0.2 μm filter paper to obtain a fermented oat sprout extract.

[0071] Concentration step (S30): The above sprouted oat extract fermentation was concentrated using a vacuum concentrator to 70 brix.

[0072]

[0073] Comparative Example 1. Preparation of sprout oat extract

[0074] Samples for the experiment were prepared according to the following process.

[0075] Step for obtaining sprouted oat extract (S10): Sprouted oats were mixed with purified water at a weight ratio of 1:20, extracted at a temperature of 90°C for 8 hours, and filtered to obtain a sprouted oat extract.

[0076] Concentration step (S20): The above sprouted oat extract was concentrated using a vacuum concentrator to 70 brix.

[0077]

[0078] Experimental Example 1. Cell Viability Confirmation (MTT Assay)

[0079] 1) Experimental method

[0080] HaCaT cells were seeded at 1x10 in a 24-well plate. 5 cells / ml concentration and cultured for 24 hours in a 37℃, 5% CO2 incubator. After removing the medium, the samples of Example 1 and Comparative Example 1 were treated at various concentrations, and 450 μl of the medium was treated each, and cultured for 24 hours in a 37℃, 5% CO2 incubator. MTT reagent was prepared, diluted to 1 mg / ml, and 100 μl per well was treated, and cultured for 2 hours in a 37℃, 5% CO2 incubator under dark conditions. After removing the supernatant, 300 μl of DMSO was treated per well, and then treated in the dark at room temperature for 5 minutes. After adding 100 μl to each 96-well plate, the absorbance was measured at a wavelength of 540 nm, and the cell viability was calculated by the following mathematical equation 1.

[0081]

[0082] 2) Experimental results

[0083] The experimental results are shown in Fig. 1 and Table 1.

[0084] Cell viability (%) Concentration (%) Example 1 Comparative example 10 100.0 100.00.01 100.7 100.20.025 101.9 100.30.05 109.2 100.60.1 116.6 100.60.25 111.8 100.90.5 108.4 101.4

[0085] As a result, no significant cytotoxicity was observed in either Example 1 or Comparative Example 1. Therefore, the composition according to the present invention was confirmed to be safe for skin cells.

[0086] Experimental Example 2. Confirmation of moisturizing effect (HA production)

[0087] 1) Experimental method

[0088] First, regarding cell culture and sample processing, HaCaT cells were seeded at 1x10 in a 24-well plate. 5 The cells / ml concentration was cultured for 24 hours in a 37℃ 5% CO2 incubator. After removing the medium, the samples of Example 1 and Comparative Example 1 were treated at various concentrations, and 450 μl of the medium was treated each, and cultured for 24 hours in a 37℃ 5% CO2 incubator. After 24 hours of culture, the supernatant was collected, centrifuged, and used as a moisture measurement sample. Moisture measurement was performed using R&D SYSTEMS, Hyaluronan ELISA Kit-Quantikine. Assay diluent and each sample were placed in a 96-well plate, and incubated with stirring at room temperature for 2 hours. After washing four times with wash buffer, 100 μl of conjugate was added, and incubated with stirring at room temperature for 2 hours. After washing five times with wash buffer, 100 μl of substrate solution was added, and incubated for 30 minutes at room temperature. 100 μl of stop solution was added, and the absorbance was measured at 450 nm. The hyaluronan increase rate was calculated by substituting the sample absorbance into the standard calibration curve.

[0089] 2) Experimental results

[0090] The experimental results are shown in Fig. 2 and Table 2.

[0091] HA production (%) Concentration (ppm) Example 1 Comparative example 10 100.0 100.010 100.3 100.2 25 104.7 101.5 50 105.0 103.3 100 106.8 105.9 250 125.1 109.5 500 131.6 111.7 1,000 135.6 112.4

[0092] When the sprouted oat extract fermentation of Example 1 was treated, the production of hyaluronan increased in a concentration-dependent manner, and at a concentration of 1,000 ppm, the amount of hyaluronan produced increased by 35.6%, confirming its effectiveness in moisturizing. In contrast, the sprouted oat extract of Comparative Example 1 showed significantly lower values.

[0093] Experimental Example 3. Astringent Verification

[0094] 1) Experimental method

[0095] Samples of Example 1 and Comparative Example 1 at various concentrations and 0.2% hemoglobin solution were added to an E-tube at a 1:1 ratio and mixed. After vortexing for approximately 10 seconds, coagulated blood proteins were sedimented using a centrifuge. 200 μl were dispensed into each 96-well plate, and the absorbance was measured at 576 nm.

[0096] 2) Experimental results

[0097] The experimental results are shown in Fig. 3 and Table 3.

[0098] Astringency (%) Concentration (%) Example 1 Comparative Example 1 TA6 1.76 1.7 0.1 36.3 2 1.4 0.5 4 3.5 3 3.9 1 5 5.7 4 1.2 TA: Tannic acid 1,000 ppm

[0099] As a result, it was confirmed that hemoglobin increased in a concentration-dependent manner and had strong activity when treated with the fermented oat sprout extract. In contrast, Comparative Example 1, which was an oat sprout extract, showed significantly lower values.

[0100] Experimental Example 4. Confirmation of Elastase Inhibition Effect (Elastase Inhibition Assay)

[0101] 1) Experimental method

[0102] 100 μL of 0.2 mol / L tris-HCl buffer, 25 μL of 10 mmol / L N-(methoxysuccinyl)-ala-ala-pro-val-4-nitroanilide (substrate), and various concentrations of the samples of Example 1 and Comparative Example 1 were mixed and incubated for 15 minutes. 25 μL of 0.3 units / mL elastase was added and incubated for an additional 15 minutes. The inhibition rate was then calculated by measuring at 410 nm using a microplate reader.

[0103] 2) Experimental results

[0104] The experimental results are shown in Fig. 4 and Table 4.

[0105] Elastase inhibition activity (%) Concentration (%) Example 1 Comparative Example 1 UA68.868.80.122.714.50.541.534.7159.843.3 UA:Ursolic acid 250 ppm

[0106] As a result, it was confirmed that the elastase inhibitory activity increased in a concentration-dependent manner when treated with the fermented oat sprout extract and had strong activity. In comparison, the oat sprout extract of Comparative Example 1 showed significantly lower values.

[0107] Experimental Example 5. Confirmation of collagenase inhibition effect (Collagenase inhibition assay)

[0108] 1) Experimental method

[0109] Various concentrations of the samples of Example 1 and Comparative Example 1 and 150 μl of collagenase (0.2 mg / ml) were added to 250 μl of a substrate solution containing 4 mM CaCl2 and 4-phenylazobenzyloxycarbonyl-Pro-Leu-Gly-Pro-Arg (0.3 mg / ml) in 1 M Tris-HCl buffer (pH 7.5), incubated at room temperature for 20 minutes, and the reaction was stopped by adding 500 μl of 6% citric acid. After adding 1.5 ml of ethylene glycol, the supernatant was collected and the absorbance was measured at 320 nm using a spectrophotometer. The inhibitory activity of collagenase was expressed as the rate of decrease in absorbance between the groups with and without the addition of the sample solution.

[0110] 2) Experimental results

[0111] The experimental results are shown in Fig. 5 and Table 5.

[0112] Collagenase inhibition activity (%) Concentration (%) Example 1 Comparative example 1 AA62.768.80.133.422.70.557.141.5171.559.8 AA: Ascorbic acid 100 ppm

[0113] As a result, the collagenase inhibitory activity was higher when treated with the fermented oat sprout extract than when treated with ascorbic acid, which was the positive control, and it was confirmed that the fermented oat sprout extract had an anti-wrinkle effect by inhibiting the activity of collagenase, a collagen-decomposing enzyme. In comparison, the fermented oat sprout extract of Comparative Example 1 showed a significantly lower value.

[0114] Experimental Example 6. Confirmation of Antioxidant Effect

[0115] 1) Experimental method

[0116] First, a 0.2 mM DPPH reagent was prepared. Samples from Example 1 and Comparative Example 1 at various concentrations were diluted in MeOH to prepare the solution. A positive control was prepared with 100 ppm A-ascorbic acid. Each sample was added to a 96-well plate in triplicate (100 μl each), followed by 100 μl of DPPH reagent. The plate was then incubated at room temperature in the dark for 30 minutes, followed by measurement at 517 nm.

[0117] 2) Experimental results

[0118] The experimental results are shown in Fig. 6 and Table 6.

[0119] DPPH radical scavenging (%) Concentration (%) Example 1 Comparative Example 1 AA92.492.40.0519.111.80.132.521.70.2561.747.60.572.459.4184.167.1 AA: ascorbic acid 100 ppm

[0120] It was confirmed that antioxidant activity increased in a concentration-dependent manner when treated with fermented oat sprout extract, and very high antioxidant activity was observed. In comparison, Comparative Example 1, which is an oat sprout extract, showed significantly lower values.

[0121] Experimental Example 7. Increase in cell binding capacity (Western blot)

[0122] 1) Cell culture and sample processing

[0123] HaCaT cells were seeded at 1x10 in a 6-well plate. 5 The samples were cultured for 24 hours in a 37°C, 5% CO2 incubator at a concentration of 10 cells / ml. After removing the medium, the samples of Example 1 and Comparative Example 1 were treated with 450 μl of medium at various concentrations, and then cultured for 24 hours in a 37°C, 5% CO2 incubator.

[0124] 2) Protein extraction

[0125] The 6-well plate on which the cells were cultured was washed once with 5 ml of cold phosphate-buffered saline (1x PBS) and the supernatant was removed. 200 μl (can be changed depending on the situation) of a solution prepared in the ratio of 1x RIPA buffer (100): Protease inhibitor (1): 100 mM PMSF (1) was dispensed and incubated at 4°C for 10 minutes. The cells were scraped with a scraper and collected in a 1.5 ml tube, and the vortex stirring and sonication were repeated three times for 30 seconds each. The cells were centrifuged at 16,000 g at 4°C for 20 minutes and the supernatant was transferred to a new 1.5 ml tube. Proteins were quantified using BCA assay.

[0126] 3) Western blot

[0127] An SDS-PAGE gel was prepared and 20 μl of protein samples were loaded. Electrophoresis was performed at 70 V for 20 minutes and 100 V for 70 minutes. Transfer was performed to a nitrocellulose membrane. After blocking in 5% skim milk, the primary antibody was added and incubated overnight at 4°C. After washing three times with 1X TBST, the secondary antibody was added and incubated at room temperature for 1 hour. After washing three times with 1X TBST, the bands were confirmed by reacting with ECL solution.

[0128] 4) Experimental results

[0129] The experimental results are as shown in Figures 7 to 9 and Tables 7 and 8.

[0130] Claudin-1 Increase Rate (%) Biomarker Concentration (%) Example 1 Comparative Example 1 Claudin-10 100.0 100.0 1010 5.4 100.1 50 112.7 101.3 100 123.5 105.9 2 50 125.9 108.6 500 131.8 111.4

[0131] Desmoglein 2 Increase Rate (%) Biomarker Concentration (%) Example 1 Comparative Example 1 Desmoglein 2 0 100.0 100.0 10103.6 101.4 50 107.2 102.4 100 111.5 103.2 250 116.8 107.4 500 122.9 110.6

[0132] As a result, it was confirmed that the expression level of Claudin-1 increased by 31.8% and the expression level of Desmoglein 2 increased by 22.9% in the group treated with 500 ppm of fermented sprout oat extract compared to the untreated group. It was confirmed that the expression levels of Claudin-1 and Desmoglein 2, which are involved in cell cohesion, increased further in Example 1, which underwent fermentation, compared to Comparative Example 1, which was not fermented. Accordingly, it was confirmed that the fermented sprout oat extract was effective in cell cohesion, and this means that as cell cohesion increases, the pore reduction effect increases.

[0133] Experimental Example 8. Sebum Control and Fat Differentiation Inhibition Activity

[0134] 1) Cell culture and sample processing

[0135] 3T3-L1 cells were seeded at 2x10 in a 6-well plate. 5 The cells were cultured at a concentration of 10 cells / well in a 37℃, 5% CO2 incubator for 48 hours. After treatment with adipogenic differentiation inducers such as 1 μM Dexamethasone, 0.5 mM IBMX, and 10 μg / ml Insulin, the cells were cultured for 48 hours. After treatment with 10 μg / ml Insulin, the medium was changed three times at 2-day intervals to induce adipogenic differentiation. After induction of adipogenic differentiation, the cells were stained with Oil Red O dye reagent and the absorbance was measured at a wavelength of 520 nm.

[0136] 2) Experimental results

[0137] The experimental results are shown in Fig. 10 and Table 9.

[0138] Liquid accumulation (%) Sample (㎍ / ml) MDI Example 1 Comparative Example 1--48.246.7-+100.0100.0P.C+83.683.3100+92.997.8500+85.394.11,000+77.188.3MDI: Fat differentiation inducer, PC: Positive control (Garcinia cambogia extract powder 1,000 ppm)

[0139] It was confirmed that when the sprout oat extract fermentation was treated, intracellular fat accumulation was reduced compared to the group treated with only a fat-inducing substance (MDI). In contrast, Comparative Example 1, which is an oat sprout extract, showed a significantly lower value. Meanwhile, as shown in Fig. 10, the part dyed red is fat, and it was confirmed that when the sprout oat extract fermentation was treated, intracellular fat accumulation was reduced compared to the group treated with only a fat-inducing substance (MDI).

[0140] Experimental Example 9. Sebum Control_Biomarker Verification

[0141] 1) Cell culture and sample processing

[0142] 3T3-L1 cells were seeded at 2x10 in a 6-well plate. 5 The cells were cultured at a concentration of 10 cells / well in a 5% CO2 incubator at 37°C for 48 hours. After treatment with adipogenic differentiation inducers such as 1 μM Dexamethasone, 0.5 mM IBMX, and 10 μg / ml Insulin, the cells were cultured for 48 hours. After treatment with 10 μg / ml Insulin, the medium was changed three times at two-day intervals to induce adipogenic differentiation.

[0143] 2) Protein extraction

[0144] The 6-well plate on which the cells were cultured was washed once with 5 ml of cold phosphate buffered saline (1x PBS) and the supernatant was removed. 200 μl (can be changed depending on the situation) of a solution prepared in the ratio of 1x RIPA buffer (100): Protease inhibitor (1): 100 mM PMSF (1) was dispensed and incubated at 4°C for 10 minutes. The cells were scraped with a scraper and collected in a 1.5 ml tube, and the vortex stirring and sonication were repeated three times for 30 seconds each. The cells were centrifuged at 16,000 g at 4°C for 20 minutes and the supernatant was transferred to a new 1.5 ml tube. The protein was quantified using BCA assay.

[0145] 3) Western blot

[0146] SDS-PAGE gels were prepared and 20 μl of protein samples were loaded. Electrophoresis was performed at 70 V for 20 minutes and 100 V for 70 minutes. Transfer was performed to nitrocellulose membranes. After blocking in 5% skim milk, primary antibodies were added and incubated overnight at 4°C. After washing three times with 1X TBST, secondary antibodies were added and incubated at room temperature for 1 hour. After washing three times with 1X TBST, bands were identified by reacting with ECL solution.

[0147] 4) Experimental results

[0148] The experimental results are as shown in Figures 12 to 14 and Tables 10 and 11.

[0149] PPAR γ expression amount (%) Biomarker Sample (㎍ / ml) MDI Example 1 Comparative Example 1 PPAR γ--14.1 13.9-+100.0 100.0 P.C+11.3 11.4 100+59.7 67.2 500+52.4 62.9 1,000+40.2 56.8 MDI: Adipogenesis-inducing substance, PC: N-Acetyl-L-Cysteine ​​1,000 ppm

[0150] C / EBP α expression level (%) Biomarker Sample (㎍ / ml) MDI Example 1 Comparative Example 1 C / EBP α--4.94.8-+100.0100.0P.C+4.34.6100+58.165.2500+51.962.71,000+46.659.4MDI: Adipogenic differentiation inducer, PC: N-Acetyl-L-Cysteine ​​1,000 ppm

[0151] As a result, it was confirmed that the expression levels of sebum-regulating factors PPAR γ and C / EBP α decreased in the group treated with the fermented sprout oat extract compared to the MDI alone treatment group. The PPAR γ factor decreased in a concentration-dependent manner with the fermented sprout oat extract, and in particular, the expression level was confirmed to decrease by 59.8% at a concentration of 1,000 ppm. The C / EBP α factor decreased in a concentration-dependent manner with the fermented sprout oat extract, and in particular, the expression level was confirmed to decrease by 53.4% ​​at a concentration of 1,000 ppm.

[0152]

[0153] Experimental Example 10. Moisturizing_Simple Clinical Trial

[0154] 1) Preparation of formulation

[0155] To conduct a moisturizing simple clinical trial, formulations as shown in Table 12 below were prepared.

[0156] Comparative Example 2 Manufacturing Example 1 Comparative Example 3 Comparative Example 4 Gly 0550 HD 0220 Ethylhexylglycerin 00.10.10 Fermented sprouted oat extract 0500 Sprouted oat extract 0050 Hyaluronic acid 0001 Purified water 100 87.987.999 Total 100 100 100 100

[0157] 2) Experimental method The number of test subjects was 3, and the measurement intervals were before application, immediately after application, 10 minutes, 20 minutes, 30 minutes, 40 minutes, 50 minutes, and 60 minutes, and measurements were made using Cutometer®MPA 580, Courage + Khazaka, Cologne Germany. 3) Experimental results

[0158] The results are shown in Fig. 15 and Table 13.

[0159] Moisturizing effect (%) Before application Immediately after application 10 minutes 20 minutes 30 minutes 40 minutes 50 minutes 60 minutes Average Comparison Example 20.0 44.3 14.7 6.03 43.01 20.8 0.046.0 15.43 53.12 91.30 60.043.11 3.84 93.12 81.30.7 Average 0.044.5 14.6 4.8 3.22 .91.30.7 Manufacturing Example 10.061.256.249.537.732.129.228.90.062.155.852.339.533.629.627.60.060.756.549.543.835.129.426.9 Average 0.061.356.250.540.433.629. 427.8Comparative Example 20.061.256.249.537.732.129.228.90.062.155.852.339.533.629.627.60.060.756.549.543.835.129.426.9Average 0.060.953.745.636.727.521.71 8.6 Comparative Example 30.055.648.642.635.625.317.815.60.054.949.641.636.126.119.816.80.053.649.043.534.224.918.613.1 Average 0.054.749.142.635.325.418.715.2

[0160] As a result, the formulation of Manufacturing Example 1, which includes the fermented sprout extract of Example 1 according to the present invention, showed the best moisturizing effect. In comparison, the remaining formulations not including Example 1 showed significantly inferior moisturizing effects. Hereinafter, based on the results of the above experimental examples, a cosmetic containing the fermented sprout extract of oat is formulated and presented as follows. However, the composition of the present invention is not limited to the following formulation examples.

[0161]

[0162] Formulation Example 1. Preparation of a toner containing fermented sprout oat extract

[0163] Among the cosmetics containing the above Example 1, a prescription example of a toner (skin lotion) is as follows.

[0164] Number of raw materials (weight%) 1 Example 15.02 Glycerin 3.03 Butylene glycol 2.04 Propylene glycol 2.05 Polyoxyethylene hydrogenated castor oil 1.06 Ethanol 10.07 Triethanolamine 0.18 Preservative trace 9 Color trace 10 Flavor trace 11 Purified water trace

[0165] <Manufacturing method> Add 2, 3, 4, and 8 in order to 11 and stir to dissolve, then heat 5 to about 60℃ to dissolve, then add 10 and dissolve, and then add to 11. Finally, add 6, 7, and 9, but add 1 and stir well before maturing.

[0166]

[0167] Formulation Example 2. Preparation of a nutritional lotion containing fermented sprout oat extract

[0168] Among the cosmetics containing the above Example 1, a prescription example of a nutritional lotion is as follows.

[0169] Number Raw material content (weight%) 1 Example 15.02 Beeswax 1.03 Polysorbate 60 1.54 Sorbitan sesquioleate 0.55 Liquid paraffin 10.06 Sorbitan stearate 1.07 Lipid monostearate Glycerin 0.58 Stearic acid 1.59 Glyceryl stearate / PEG-400 stearate 1.0 10 Propylene glycol 3.0 11 Carboxypolymer 0.1 12 Triethanolamine 0.2 13 Preservative trace 14 Color trace 15 Flavor trace 16 Purified water trace

[0170] <Manufacturing method> Mix and stir 10, 11, 12, 13, and 16 and heat to 80 to 85℃, then add to the manufacturing section, operate the emulsifier, heat 2, 3, 4, 5, 6, 7, 8, 9, and 12 to 80 to 85℃ to dissolve, and then emulsify. After emulsification is complete, cool to 50℃ while stirring using a stirrer, then add No. 15, cool to 45℃, add No. 14, add No. 1 at 35℃, cool to 25℃, and then mature.

[0171]

[0172] Formulation Example 3. Preparation of a nutritional cream containing fermented sprout oat extract

[0173] Among the cosmetics containing the above Example 1, a prescription example of a nutritional cream is as follows.

[0174] Number Raw material content (weight%) 1 Example 15.02 Stearic acid 2.03 Cetyl alcohol 2.04 Glyceryl monostearate 2.05 Polyoxyethylene sorbitan monostearate 0.56 Sorbitan sesquioleate 0.57 Glyceryl monostearate / glyceryl stearate / polyoxyethylene stearate 1.08 Wax 1.09 Liquid paraffin 4.0 10 Squalane 4.0 11 Caprylic / capric triglyceride 4.0 12 Carboxyvinyl polymer 0.3 13 Butylene glycol 5.0 14 Glycerin 3.0 15 Triethanolamine 0.5 16 Residual water content

[0175] <Manufacturing method> Mix and stir 12, 13, 14, and 16 and heat to 80 to 85℃, then add to the manufacturing section, operate the emulsifier, heat 2, 3, 4, 5, 6, 7, 8, 9, 10, and 11 to 80 to 85℃ and dissolve, then add 15, stir, and add to the manufacturing section to emulsify. After emulsification is complete, cool to 35℃ while stirring using a stirrer, add 1, cool to 25℃, and mature.

[0176]

[0177] Formulation Example 4. Preparation of essence containing fermented sprout oat extract

[0178] Among the cosmetics containing the above Example 1, the prescription examples of essence are as follows.

[0179] Number of raw materials (weight%) 1 Example 15.02 Cytosterol 1.73 Polyglyceryl 2-oleate 1.54 Ceramide 0.75 Steareth-4 1.26 Cholesterol 1.57 Dicetyl phosphate 0.48 Concentrated glycerin 5.09 Macadamia oil 15.0 10 Carboxyvinyl polymer 0.2 11 Xanthan gum 0.2 12 Preservative trace 13 Flavor trace 14 Remaining tablet amount

[0180] <Manufacturing method> 2, 3, 4, 5 and 6 are homogenized at a constant temperature and called a nonionic amphiphilic lipid. The nonionic amphiphilic lipid and 7, 8 and 14 are mixed, and 1 is added and mixed, homogenized at a constant temperature and passed through a microfluidizer, and then 9 is slowly added at a constant temperature and homogenized and passed through a microfluidizer again. Then, 10, 11, 12 and 13 are added and dispersed to stabilize and mature.

[0181]

[0182] Although the present invention has been described with reference to the attached drawings, this is only one embodiment among various embodiments including the gist of the present invention, and the purpose is to enable those skilled in the art to easily practice it, and it is clear that the present invention is not limited to the embodiments described above. Therefore, the protection scope of the present invention should be interpreted by the following claims, and all technical ideas within the equivalent scope by modification, substitution, replacement, etc., within the scope that does not depart from the gist of the present invention, will be included in the rights of the present invention. In addition, it is clearly stated that some components of the drawings are provided in an exaggerated or reduced form compared to the actual structure to more clearly explain the components.

[0183]

[0184] [Explanation of symbols]

[0185] (S10): Step for obtaining sprout oat extract

[0186] (S20): Step for obtaining fermented oat sprout extract

[0187] (S30): Concentration stage

Claims

1. A cosmetic composition comprising a fermented oat sprout extract, characterized in that it comprises a fermented oat sprout extract obtained by fermenting a sprout extract obtained by extracting sprout oats.

2. In paragraph 1, A cosmetic composition comprising a fermented oat sprout extract, characterized in that the above oat sprout extract is obtained by mixing oat sprout and purified water in a weight ratio of 1:20, extracting at a temperature of 80 to 100°C for 6 to 10 hours, and filtering.

3. In paragraph 1, A cosmetic composition comprising a fermented oat sprout extract, characterized in that the fermented oat sprout extract is obtained by fermenting the fermented oat sprout extract using a Galactomyces candidus strain for 12 to 50 hours at a temperature of 25 to 40°C and filtering the fermented oat sprout extract.

4. In paragraph 1, A cosmetic composition comprising a fermented oat sprout extract, characterized in that the fermented oat sprout extract is concentrated using a vacuum concentrator to a brix of 50 to 90.

5. Step of obtaining sprouted oat extract by extracting sprouted oats (S10); A step (S20) of obtaining a fermented oat sprout extract by fermenting the above-mentioned oat sprout extract; and, A method for producing a cosmetic composition comprising a fermented oat sprout extract, characterized in that it comprises a concentration step (S30) of concentrating the fermented oat sprout extract using a vacuum concentrator.

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