Use of small molecule compound in increasing content of cannabidiol and tetrahydrocannabinol in cannabis

Abstract

The use of a small molecule compound in increasing the content of cannabidiol and tetrahydrocannabinol in cannabis. Experiments verify that a low concentration of RPS-1 can increase the content of CBD and THC in planted cannabis and, in practical production and use, reduce the production costs of CBD and THC. RPS-1 is exogenously sprayed and flexibly applied. The method does not need to change the variety and genetic background of cannabis, and can be applied to cannabis of all varieties.

Description

Application of small molecule compounds in improving cannabidiol and tetrahydrocannabinol content in cannabis TECHNICAL FIELD

[0001] The present application relates to the field of agricultural technology, in particular to the application of small molecule compounds in improving cannabidiol and tetrahydrocannabinol content in cannabis. BACKGROUND

[0002] Cannabidiol (CBD) and tetrahydrocannabinol (THC) are the main chemical components in cannabis plants, mainly extracted from female cannabis plants. CBD is a non-psychoactive component in cannabis, which has various pharmacological effects such as anticonvulsant, anxiolytic, and anti-inflammatory effects. THC is the main psychoactive component in cannabis. Traditionally, CBD and THC content in cannabis varieties is improved through hybrid breeding. The success of new variety breeding has greatly improved the planting level and economic value of cannabis.

[0003] However, the cultivation of new varieties often requires a long period and a large amount of expenditure. In addition, due to the particularity of the variety, special methods are required for seedling and planting during the cultivation of the new variety, thereby increasing the cost of cultivation. In addition, the improvement of cannabis varieties through traditional breeding, genetic modification or gene editing technology also involves variety approval and supervision, and the review period usually takes several years, with a cost of tens of millions of yuan.

[0004] Therefore, it is urgent to develop a regulator that does not need to change the cannabis variety and genetic background, can be directly applied to existing industrial cannabis varieties, and can improve the content of CBD and THC in a short period of time, thereby reducing the production cost. SUMMARY

[0005] In view of the defects in the prior art, the present application provides the application of small molecule compounds in improving the content of cannabidiol in cannabis, which improves the content of CBD and THC in cannabis by applying small molecule compound RPS-1.

[0006] The present application provides the application of small molecule compounds selected from any one of (1) to (3) in improving the content of CBD in cannabis;

[0007] (1) a compound of formula (I) or a salt or ester thereof;

[0008] (2) an optical isomer of the compound of formula (I) or a racemate thereof;

[0009] (3) a solvate of the compound of formula (I) or a precursor thereof;

[0010] The present application also provides the application of small molecule compounds selected from any one of (1) to (3) in improving the content of CBD and THC in cannabis.

[0011] The small molecule compound is named as RPS-1, and the present application increases the accumulation of secondary metabolites CBD and THC by activating the self-related signal pathway of cannabis by spraying RPS-1 once or more times in the vegetative growth stage and reproductive growth stage of cannabis.

[0012] In some embodiments, the small molecule compound of formula (I) can be formulated into flowable composition, wettable powder, microemulsion, suspension, granule, slow-release agent or a combination thereof and similar dosage forms. All dosage forms are suitable for soil, water and / or foliar application.

[0013] In some embodiments, the cannabis is any one of industrial hemp, recreational cannabis and medical cannabis.

[0014] In some embodiments, the industrial hemp is any one of the cultivated strains belonging to Cannabis sativa L. and Cannabis indica Lam. and hybrids thereof.

[0015] In some embodiments, the recreational cannabis and medical cannabis are any one of the cultivated strains of Cannabis sativa L. and Cannabis indica Lam. and their mutual hybrids.

[0016] The present application also provides the use of any one selected from (1) to (3) in the preparation of a preparation for increasing the content of CBD and THC in cannabis:

[0017] (1) the compound of formula (I) or its salt or ester;

[0018] (2) the optical isomer of the compound of formula (I) or its racemate;

[0019] (3) the solvate of the compound of formula (I) or its precursor;

[0020] In some embodiments, the cannabis is one of industrial hemp, recreational cannabis or medical cannabis.

[0021] In some embodiments, the preparation further comprises any one or more of surfactants, humectants, spreading agents, sticking agents, antifreezing agents, synergists and corrosion inhibitors.

[0022] In some embodiments, the surfactant is preferably any one of a cationic surfactant, an anionic surfactant, an amphoteric surfactant, or a non-ionic surfactant; and the content of the surfactant is 0.01-10 wt%.

[0023] In some embodiments, the preparation can comprise other active ingredients in addition to the small-molecule compound of Formula (I). For example, active ingredients that can kill fungi can be mixed, or other plant growth regulator ingredients can be mixed.

[0024] The present application also provides a method for increasing the content of CBD and THC in cannabis, wherein the small-molecule compound is sprayed one or more times during any one or both of the vegetative growth stage and the reproductive growth stage of the cannabis.

[0025] In some embodiments, the spraying method comprises foliar spraying or root irrigation.

[0026] In some embodiments, the concentration of the small-molecule compound is 10-500 μM, preferably 50-200 μM.

[0027] In some embodiments, the amount of a single spraying is 1-100 L / acre of cannabis, preferably 2-50 L / acre of cannabis.

[0028] In summary, compared with the prior art, the present application achieves the following technical effects:

[0029] 1. The present application provides a new use of the small-molecule compound RPS-1 for increasing the content of CBD and THC in cannabis, and the method of the present application does not need to change the variety and genetic background of cannabis.

[0030] 2. The present application has been verified by tests that the small-molecule compound RPS-1 can increase the content of CBD and THC in industrial cannabis at a lower concentration, thereby reducing the production cost of CBD and THC in actual production and application.

[0031] 3. The small-molecule compound RPS-1 of the present application is exogenous and can be sprayed flexibly, and can be applied to all varieties of cannabis, including industrial cannabis, recreational cannabis, and medical cannabis. BRIEF DESCRIPTION OF DRAWINGS

[0032] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed in the embodiments will be briefly introduced below, and it should be understood that the following drawings only show some embodiments of the present application, and therefore should not be regarded as a limitation on the scope. For those skilled in the art, other related drawings can also be obtained without creative labor on the basis of these drawings.

[0033] Figure 1 is the CBD content detection result of the present application examples 1-9, 16-18 and comparative examples 1-3, 6;

[0034] Figure 2 is the CBD content detection result of the present application comparative examples 4-5 and examples 10-15;

[0035] Figure 3 is the CBD content detection result of the present application comparative examples 1, 6 and examples 20-23, 29-33;

[0036] Figure 4 is the CBD content detection result of the present application comparative example 4 and examples 10, 24-28. DETAILED DESCRIPTION

[0037] In order to make the personnel in the technical field better understand the present application scheme, the technical scheme in the present application examples will be described clearly and completely in combination with the drawings in the present application examples. Obviously, the described examples are only a part of the present application examples, not all. Based on the examples in the present application, all other examples obtained by the person skilled in the art without creative labor should belong to the scope of the present application protection.

[0038] The present application sprays RPS-1 once or more times in the vegetative growth stage and reproductive growth stage of hemp, activates the signal pathway of industrial hemp itself, thereby increasing the accumulation of primary and secondary metabolites, and finally realizes the increase of CBD and THC content. According to the results of the examples, RPS-1 is suitable for multiple industrial hemp varieties, and can increase the content of CBD and THC at low dose.

[0039] The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents, etc. used are commercially available unless otherwise specified.

[0040] Material source:

[0041] Guomai No. 1: Harbin Hanbo Biological Technology Co., Ltd.;

[0042] Muma No. 3: Harbin Hanbo Biological Technology Co., Ltd.;

[0043] Hanma No. 7: Harbin Hanbo Biological Technology Co., Ltd.;

[0044] White Widow: Royal Queen Seeds Co.;

[0045] Sour Diesel: Royal Queen Seeds Co.;

[0046] Candida (CD-1): Kind Seed Co.;

[0047] RPS-1: Preparation and identification of the compound are described in detail in Patent No. ZL201611066259.1.

[0048] 1. Cultivation management of cannabis

[0049] Five varieties of cannabis, namely, Guomai No. 1, Hanma No. 7, Mumama No. 3, White Widow, and Candida (CD-1), were selected for field planting. The most suitable soil for the cultivation of cannabis is sandy loam with deep soil layer, loose and fertile soil. Clay soil is not suitable for the cultivation of cannabis because of poor drainage, which can cause poor emergence, root rot, and seedling death of cannabis.

[0050] The growth cycle refers to the autumn and winter planting season in the temperate continental monsoon climate production area, as shown in Table 1:

[0051] Table 1. Growth cycle of industrial hemp (winter planting season)

[0052] 2. Preparation of RPS-1

[0053] RPS-1 is dissolved in an organic solvent (dimethyl sulfoxide (DMSO) is used in the examples) to prepare a storage solution (e.g., 200 mM), which is then diluted with water to the desired concentration (usually 1-500 μM) and a surfactant (0.1% (v / v) Tween-20 is used in the examples) is added.

[0054] Spraying can be performed using artificial, unmanned aerial vehicles, or other field spraying equipment, or through daily drip irrigation.

[0055] 3. Treatment of cannabis with RPS-1 solution

[0056] Spraying is performed during the vegetative and reproductive growth periods of cannabis, with the vegetative growth period being preferred. The spraying is performed once or multiple times.

[0057] Example 1

[0058] Fifty μM RPS-1 solution was sprayed twice on the leaves of Guomai No. 1 at the early and vigorous growth stages, respectively. The spraying area was 1 mu, and the spraying amount was 30 L per mu each time, with an interval of 20 days.

[0059] Example 2

[0060] Fifty μM RPS-1 solution was sprayed twice on the leaves of Guomai No. 1 at the early and vigorous growth stages, respectively. The spraying area was 1 mu, and the spraying amount was 30 L per mu each time, with an interval of 45 days.

[0061] Example 3

[0062] Spray 50 μM RPS-1 solution twice to the leaves of Guomax No. 1 at vigorous vegetative growth stage and early reproductive growth stage, respectively, with spraying area of 1 mu, spraying dosage of 30 L per mu each time, and interval of 25 days.

[0063] Example 4

[0064] Spray 50 μM RPS-1 solution twice to the leaves of Mumax No. 3 at early and vigorous vegetative growth stage, respectively, with spraying area of 1 mu, spraying dosage of 30 L per mu each time, and interval of 20 days.

[0065] Example 5

[0066] Spray 50 μM RPS-1 solution twice to the leaves of Mumax No. 3 at early and vigorous vegetative growth stage, respectively, with spraying area of 1 mu, spraying dosage of 30 L per mu each time, and interval of 45 days.

[0067] Example 6

[0068] Spray 50 μM RPS-1 solution twice to the leaves of Mumax No. 3 at vigorous vegetative growth stage and early reproductive growth stage, respectively, with spraying area of 1 mu, spraying dosage of 30 L per mu each time, and interval of 25 days.

[0069] Example 7

[0070] Spray 50 μM RPS-1 solution twice to the leaves of Hanma No. 7 at vigorous vegetative growth stage and early reproductive growth stage, respectively, with spraying area of 1 mu, spraying dosage of 30 L per mu each time, and interval of 20 days.

[0071] Example 8

[0072] Spray 50 μM RPS-1 solution twice to the leaves of Hanma No. 7 at vigorous vegetative growth stage and early reproductive growth stage, respectively, with spraying area of 1 mu, spraying dosage of 30 L per mu each time, and interval of 45 days.

[0073] Example 9

[0074] Spray 50 μM RPS-1 solution twice to the leaves of Hanma No. 7 at vigorous vegetative growth stage and early reproductive growth stage, respectively, with spraying area of 1 mu, spraying dosage of 30 L per mu each time, and interval of 25 days.

[0075] Example 10

[0076] Spray 50 μM RPS-1 solution twice to the leaves of White Widow at vigorous vegetative growth stage and early reproductive growth stage, respectively, with spraying area of 1 mu, spraying dosage of 30 L per mu each time, and interval of 20 days.

[0077] Example 11

[0078] Spray 50 μM RPS-1 solution twice to the leaves of White Widow variety at vigorous vegetative growth stage and early reproductive growth stage, respectively, with a spraying area of 1 mu, a spraying amount of 30 L per mu each time, and an interval of 45 days.

[0079] Example 12

[0080] Spray 50 μM RPS-1 solution twice to the leaves of White Widow variety at vigorous vegetative growth stage and early reproductive growth stage, respectively, with a spraying area of 1 mu, a spraying amount of 30 L per mu each time, and an interval of 25 days.

[0081] Example 13

[0082] Spray 50 μM RPS-1 solution twice to the leaves of Sour Diesel variety at vigorous vegetative growth stage and early reproductive growth stage, respectively, with a spraying area of 1 mu, a spraying amount of 30 L per mu each time, and an interval of 20 days.

[0083] Example 14

[0084] Spray 50 μM RPS-1 solution twice to the leaves of Sour Diesel variety at vigorous vegetative growth stage and early reproductive growth stage, respectively, with a spraying area of 1 mu, a spraying amount of 30 L per mu each time, and an interval of 45 days.

[0085] Example 15

[0086] Spray 50 μM RPS-1 solution twice to the leaves of Sour Diesel variety at vigorous vegetative growth stage and early reproductive growth stage, respectively, with a spraying area of 1 mu, a spraying amount of 30 L per mu each time, and an interval of 25 days.

[0087] Example 16

[0088] Spray 50 μM RPS-1 solution twice to the leaves of Candida (CD-1) variety at vigorous vegetative growth stage and early reproductive growth stage, respectively, with a spraying area of 1 mu, a spraying amount of 30 L per mu each time, and an interval of 20 days.

[0089] Example 17

[0090] Spray 50 μM RPS-1 solution twice to the leaves of Candida (CD-1) variety at vigorous vegetative growth stage and early reproductive growth stage, respectively, with a spraying area of 1 mu, a spraying amount of 30 L per mu each time, and an interval of 45 days.

[0091] Example 18

[0092] Candida (CD-1) variety at the vigorous vegetative growth stage and the early reproductive growth stage, respectively, twice with 50 μM RPS-1 solution, the spraying area is 1 mu, the spraying amount is 30 L per mu each time, and the interval is 25 days.

[0093] Example 19

[0094] The leaves of Guomaca No. 1 variety are sprayed with 5 μM RPS-1 solution twice at the vigorous vegetative growth stage and the early reproductive growth stage, respectively, the spraying area is 1 mu, the spraying amount is 30 L per mu each time, and the interval is 20 days.

[0095] Example 20

[0096] The leaves of Guomaca No. 1 variety are sprayed with 25 μM RPS-1 solution twice at the vigorous vegetative growth stage and the early reproductive growth stage, respectively, the spraying area is 1 mu, the spraying amount is 30 L per mu each time, and the interval is 20 days.

[0097] Example 21

[0098] The leaves of Guomaca No. 1 variety are sprayed with 100 μM RPS-1 solution twice at the vigorous vegetative growth stage and the early reproductive growth stage, respectively, the spraying area is 1 mu, the spraying amount is 30 L per mu each time, and the interval is 20 days.

[0099] Example 22

[0100] The leaves of Guomaca No. 1 variety are sprayed with 200 μM RPS-1 solution twice at the vigorous vegetative growth stage and the early reproductive growth stage, respectively, the spraying area is 1 mu, the spraying amount is 30 L per mu each time, and the interval is 20 days.

[0101] Example 23

[0102] The leaves of Guomaca No. 1 variety are sprayed with 400 μM RPS-1 solution twice at the vigorous vegetative growth stage and the early reproductive growth stage, respectively, the spraying area is 1 mu, the spraying amount is 30 L per mu each time, and the interval is 20 days.

[0103] Example 24

[0104] The leaves of White Widow variety are sprayed with 5 μM RPS-1 solution twice at the vigorous vegetative growth stage and the early reproductive growth stage, respectively, the spraying area is 1 mu, the spraying amount is 30 L per mu each time, and the interval is 20 days.

[0105] Example 25

[0106] Spraying 5 μM RPS-1 solution twice to the leaves of White Widow variety at vigorous vegetative growth stage and early reproductive growth stage, spraying area is 1 mu, spraying amount is 30 L per mu each time, interval is 20 days.

[0107] Example 26

[0108] Spraying 100 μM RPS-1 solution twice to the leaves of White Widow variety at vigorous vegetative growth stage and early reproductive growth stage, spraying area is 1 mu, spraying amount is 30 L per mu each time, interval is 20 days.

[0109] Example 27

[0110] Spraying 200 μM RPS-1 solution twice to the leaves of White Widow variety at vigorous vegetative growth stage and early reproductive growth stage, spraying area is 1 mu, spraying amount is 30 L per mu each time, interval is 20 days.

[0111] Example 28

[0112] Spraying 400 μM RPS-1 solution twice to the leaves of White Widow variety at vigorous vegetative growth stage and early reproductive growth stage, spraying area is 1 mu, spraying amount is 30 L per mu each time, interval is 20 days.

[0113] Example 29

[0114] Spraying 5 μM RPS-1 solution twice to the leaves of Candida (CD-1) variety at vigorous vegetative growth stage and early reproductive growth stage, spraying area is 1 mu, spraying amount is 30 L per mu each time, interval is 20 days.

[0115] Example 30

[0116] Spraying 25 μM RPS-1 solution twice to the leaves of Candida (CD-1) variety at vigorous vegetative growth stage and early reproductive growth stage, spraying area is 1 mu, spraying amount is 30 L per mu each time, interval is 20 days.

[0117] Example 31

[0118] Spraying 100 μM RPS-1 solution twice to the leaves of Candida (CD-1) variety at vigorous vegetative growth stage and early reproductive growth stage, spraying area is 1 mu, spraying amount is 30 L per mu each time, interval is 20 days.

[0119] Example 32

[0120] Spray 200 μM RPS-1 solution twice to the leaf surface of Candida (CD-1) at vigorous vegetative growth stage and early reproductive growth stage, with spraying area of 1 mu, spraying dosage of 30 L per mu each time, and interval of 20 days.

[0121] Example 33

[0122] Spray 400 μM RPS-1 solution twice to the leaf surface of Candida (CD-1) at vigorous vegetative growth stage and early reproductive growth stage, with spraying area of 1 mu, spraying dosage of 30 L per mu each time, and interval of 20 days.

[0123] Comparative Example 1

[0124] Spray water containing 0.1% Tween 20 twice to the leaf surface of Guomaca No. 1 at early and vigorous vegetative growth stages, with spraying area of 1 mu, spraying dosage of 30 L per mu each time, and interval of 20 days.

[0125] Comparative Example 2

[0126] Spray water containing 0.1% Tween 20 twice to the leaf surface of Mumaca No. 3 at early and vigorous vegetative growth stages, with spraying area of 1 mu, spraying dosage of 30 L per mu each time, and interval of 20 days.

[0127] Comparative Example 3

[0128] Spray water containing 0.1% Tween 20 twice to the leaf surface of Hanmaca No. 7 at early and vigorous vegetative growth stages, with spraying area of 1 mu, spraying dosage of 30 L per mu each time, and interval of 20 days.

[0129] Comparative Example 4

[0130] Spray water containing 0.1% Tween 20 twice to the leaf surface of White willow at early and vigorous vegetative growth stages, with spraying area of 1 mu, spraying dosage of 30 L per mu each time, and interval of 20 days.

[0131] Comparative Example 5

[0132] Spray water containing 0.1% Tween 20 twice to the leaf surface of Sour Diesel at early and vigorous vegetative growth stages, with spraying area of 1 mu, spraying dosage of 30 L per mu each time, and interval of 20 days.

[0133] Comparative Example 6

[0134] Spray water containing 0.1% Tween 20 twice to the leaf surface of Candida (CD-1) at early and vigorous vegetative growth stages, with spraying area of 1 mu, spraying dosage of 30 L per mu each time, and interval of 20 days.

[0135] 4. Harvesting of industrial hemp

[0136] (1) Normal harvesting of hemp at full flowering stage, sampling at early flowering stage (sampling time point 1) and full flowering stage (sampling time point 2), respectively, taking 3 samples for each combination, and then detecting the content of CBD and THC.

[0137] (2) The appearance, growth and yield of hemp in the three treatment groups were not significantly different compared with the control group (CK).

[0138] 5. Detection of CBD content

[0139] Sample preparation:

[0140] Liquid chromatography-mass spectrometry was used to detect the content of CBD and THC. 5 g of hemp sample was finely ground, 1 g of ground sample was taken in a test tube and 30 mL of 80% methanol solution was added. Shake for 15 min, then centrifuge at 5000g for 15 min. Take 2 mL of supernatant in a needle filter, filter with 0.2 μm filter membrane. After filtration, take 30 μL of filtrate in a 1.5 mL test tube, add 970 μL of 80% methanol solution to obtain 1000-fold dilution extract.

[0141] Liquid chromatography conditions:

[0142] Chromatography column Agilent Eclipse Plus C18 column, mobile phase water (0.1% formic acid): acetonitrile (0.1% formic acid) 10:90. Flow rate 0.5 mL / min, time length 15 min. Column temperature 40℃, injection volume 20 μL. Mass spectrometry conditions:

[0143]

[0144] ​CBD, CBDA, THC and THCA were detected using SCIEX Triple Quad™ 5500 triple quadrupole mass spectrometry detection system. The detection mode was set to positive ion mode. The spray voltage was 4500V, and the ion source temperature was 600℃. Nitrogen was used as the carrier gas, and the gas source 1 and 2 pressures were both 50 psi. The mass spectrometry detection parameters were set to cone hole voltage 100V, entrance voltage 10V, and collision energy 29V. The MS / MS parameters were CBD: m / z 315.0→193.0, CBDA: m / z 341.0→219.0, THC: m / z 315.0→193.0, and THCA: m / z 341.0→219.0. The detection results were quantified using an external standard method. First, a 0.2-50ng / mL solution was prepared using a standard product to detect the corresponding ion peak intensity, and a standard curve was prepared. The sample CBD and THCA content was calculated from the ion peak area of the detection results according to the standard curve. The total CBD and THC content was the sum of the CBD and CBDA detection values and the THC and THCA detection values, and the conversion method was:

[0145] % of THC Total = % of THC + (% of THCA x 0.877)

[0146] % of CBD Total = % of CBD + (% of CBDA x 0.877)

[0147] The specific content (%) is shown in Tables 2-3:

[0148] Table 2 CBD detection results (%) of examples and comparative examples

[0149] Table 3 THC detection results (%) of examples and comparative examples

[0150] As shown in Figure 1, Examples 1, 4, 7, 16 are sprayed at 20-day intervals during the vegetative and reproductive growth periods of Guomai No. 1, Mumian No. 3, and Hanma No. 7 and Candida (CD-1). Examples 2, 5, 8, 17 are sprayed at 45-day intervals during the vegetative and reproductive growth periods of the four cannabis varieties. Examples 3, 6, 9, 18 are sprayed at 25-day intervals during the vegetative and reproductive growth periods of the four cannabis varieties. The control groups are Comparative Examples 1, 2, 3, 6, respectively. The results show that the CBD content of the examples is higher than that of the corresponding comparative examples to varying degrees, indicating that spraying RPS-1 during the vegetative and reproductive growth periods of cannabis can increase the CBD content, and the effect is better during the vegetative growth period. As shown in Figure 2, Examples 10, 13 are sprayed at 20-day intervals during the vegetative and reproductive growth periods of White willow and Sour Diesel. Examples 11, 14 are sprayed at 45-day intervals during the vegetative and reproductive growth periods of White willow and Sour Diesel. Examples 12, 15 are sprayed at 25-day intervals during the vegetative and reproductive growth periods of White willow and Sour Diesel. The control groups are Comparative Examples 4, 5. The results show that the THC content of the examples is higher than that of the corresponding comparative examples to varying degrees, indicating that spraying RPS-1 during the vegetative and reproductive growth periods of cannabis can increase the THC content, and the effect is better during the vegetative growth period.

[0151] As shown in Figure 3, the comparison of Comparative Example 1 and Examples 1, 19, 20, 21, 22, 23, and Comparative Example 6 and Examples 16, 29, 30, 31, 32, 33 shows that spraying RPS-1 at a concentration of 5 μM to 400 μM can increase the CBD content, and spraying at a concentration of 50 μM is optimal for increasing the CBD content. As shown in Figure 4, the comparison of Comparative Example 4 and Examples 10, 24, 25, 26, 27, 28 shows that spraying RPS-1 at a concentration of 5 μM to 400 μM can increase the THC content, and spraying at a concentration of 50 μM is optimal for increasing the THC content.

[0152] In summary, the small molecule compound RPS-1 has the effect of promoting the CBD and THC content of cannabis at a low concentration, and does not affect the growth of cannabis. The present application helps to increase the content of the medicinal effective components CBD and THC in industrial cannabis, which is of great significance in actual production.

[0153] The above description is only the preferred embodiments of the present application and is not intended to limit the present application. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. Use of a small molecule compound selected from any one of (1) to (3) in increasing the content of cannabidiol in cannabis. (1) a compound of formula (I) or a salt or ester thereof; (2) an optical isomer of the compound of formula (I) or a racemate thereof; (3) solvates of the compounds of formula (I) or precursors thereof; 2. Use of a small molecule compound selected from any one of (1) to (3) in increasing the content of cannabidiol and tetrahydrocannabinol in cannabis. (1) a compound of formula (I) or a salt or ester thereof; (2) an optical isomer of the compound of formula (I) or a racemate thereof; (3) solvates of the compounds of formula (I) or precursors thereof; 3. Use according to claim 1, characterized in that, The cannabis is any one of industrial cannabis, recreational cannabis and medical cannabis.

4. Use according to claim 3, characterized in that, The industrial cannabis is any one of a cultivated strain belonging to Cannabis sativa L. and Cannabis indica Lam. and hybrids thereof.

5. Use according to claim 3, characterized in that, The recreational cannabis or the medical cannabis is any one of a cultivated strain belonging to Cannabis sativa L. and Cannabis indica Lam. and hybrids thereof.

6. Use of a small molecule compound selected from any one of (1) to (3) in the preparation of a preparation for increasing the content of cannabidiol and tetrahydrocannabinol in cannabis. (1) a compound of formula (I) or a salt or ester thereof; (2) an optical isomer of the compound of formula (I) or a racemate thereof; (3) solvates of the compounds of formula (I) or precursors thereof; 7. Use according to claim 6, characterized in that, The cannabis is any one of industrial cannabis, recreational cannabis or medical cannabis.

8. Use according to claim 6 or 7, characterized in that, The preparation further comprises any one or more of a surfactant, a wetting agent, a spreading agent, an adhesion agent, an anti-freezing agent, a synergist and a corrosion inhibitor.

9. A method of increasing the content of cannabidiol and tetrahydrocannabinol in cannabis, characterized in that, The small molecule compound of claim 1 is sprayed once or more than once at any one or both of the vegetative growth stage and the reproductive growth stage of the cannabis.

10. The method of claim 9, wherein, The spraying mode comprises any one of foliar spraying and root irrigation.

11. The method of claim 9, wherein, The concentration of the small molecule compound is 10 to 500 μM.

12. The method of claim 9, wherein, The amount of single spraying is 1 to 100 L per mu of cannabis.

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