Perfusion device

Abstract

This perfusion device (1) comprises a supply pipe (31) and a body case (70). The supply pipe (31) supplies a perfusate from a reservoir (10) to an organ (9) in a reactor by means of a water head difference. The supply pipe (31) is attached to the front side of the body case (70). Accordingly, the supply pipe (31) can be easily attached to and detached from the body case (70). The reservoir (10) and a display (60) are disposed on the upper part of the body case (70). With this configuration, the lateral width and depth dimensions of the perfusion device (1) can be reduced.

Description

perfusion device

[0001] The present invention relates to a perfusion device for supplying a perfusion fluid to an organ.

[0002] In organ transplant surgery, after an organ is removed from a donor, it is temporarily stored outside the body until it is transplanted into a recipient. During this process, the organ may be preserved while a perfusion fluid is supplied to the organ to prevent the organ from becoming ischemic.

[0003] A conventional perfusion device for supplying a perfusion fluid to an organ is described in, for example, Patent Document 1.

[0004] Japanese Patent Application Laid-Open No. 2020-189890

[0005] In this type of perfusion device, the user must place and connect the piping for supplying the perfusion fluid in a predetermined position. However, in a structure in which the piping is housed within a housing, the tasks of placing and connecting the piping before using the device and removing the piping after using the device are cumbersome.

[0006] SUMMARY OF THE INVENTION It is therefore an object of the present invention to provide a perfusion device that allows easy attachment and detachment of piping.

[0007] The first invention is a perfusion device that supplies perfusion fluid to an organ, comprising: a reservoir that stores perfusion fluid; a reactor that is positioned below the reservoir and that accommodates the organ; a supply pipe that supplies perfusion fluid from the reservoir to the organ in the reactor by means of a head difference; a main body case to which the supply pipe is attached; and a display that displays the operating status of the perfusion device, wherein the supply pipe is attached to the front of the main body case, and the reservoir and the display are positioned at the top of the main body case.

[0008] A second aspect of the present invention is the perfusion device of the first aspect of the present invention, further comprising a recovery pipe for recovering perfusion fluid discharged from the organ, the recovery pipe being attached to the front surface of the main body case.

[0009] The third invention is a perfusion device of the second invention, wherein the main body case has on its front surface a first guideline indicating the position where the supply piping should be placed, and a second guideline indicating the position where the recovery piping should be placed.

[0010] A fourth aspect of the present invention is the perfusion device of the third aspect of the present invention, wherein the first guide line and the second guide line are different colors from each other.

[0011] A fifth invention is a perfusion device according to any one of the second to fourth inventions, wherein the main body case has a first holder that holds the supply piping, a first holder sensor that detects whether the supply piping is held in the first holder, a second holder that holds the recovery piping, and a second holder sensor that detects whether the supply piping is held in the second holder.

[0012] A sixth aspect of the present invention is the perfusion device of any one of the first to fifth aspects of the present invention, further comprising an elevation mechanism for changing the height of the reservoir.

[0013] The seventh invention is a perfusion device according to any one of the first to sixth inventions, further comprising a fluid volume sensor that detects whether the amount of perfusion fluid in the reservoir is greater than or equal to a predetermined amount, a valve provided in the supply pipe, and a control unit that closes the valve when the fluid volume sensor detects that the amount of perfusion fluid in the reservoir is less than the predetermined amount.

[0014] An eighth aspect of the present invention is the perfusion device of any one of the first to seventh aspects of the present invention, wherein the reactor is separable from the main body case.

[0015] According to the first to eighth aspects of the present invention, the supply pipe is attached to the front surface of the main body case. This allows the supply pipe to be easily attached and detached to and from the main body case. In addition, the reservoir and display are located at the top of the main body case. This allows the width and depth of the perfusion device to be reduced.

[0016] In particular, according to the second aspect of the present invention, the recovery pipe is attached to the front surface of the main body case, which makes it easy to attach and detach the recovery pipe to and from the main body case.

[0017] In particular, according to the third aspect of the present invention, the supply pipes are arranged along the first guide line, and the recovery pipes are arranged along the second guide line, so that the supply pipes and the recovery pipes can be arranged in the correct positions.

[0018] In particular, according to the fourth aspect of the present invention, the position where the supply pipe should be disposed and the position where the recovery pipe should be disposed can be easily distinguished.

[0019] In particular, according to the fifth aspect of the present invention, it is possible to detect whether the supply pipe and the recovery pipe are arranged on the front surface of the main body case.

[0020] In particular, according to the sixth aspect of the present invention, the pressure of the perfusion fluid due to the head difference can be adjusted by changing the height of the reservoir.

[0021] In particular, according to the seventh aspect of the present invention, when the amount of perfusion fluid in the reservoir falls below a predetermined amount, the supply of perfusion fluid to the organ can be automatically stopped.

[0022] Fig. 1 is a diagram showing the piping configuration of the perfusion device. Fig. 2 is a control block diagram of the perfusion device. Fig. 3 is a flowchart showing the flow of organ transplant surgery. Fig. 4 is a diagram showing the perfusion device as viewed from the front. Fig. 5 is a diagram showing the perfusion device as viewed from the front.

[0023] Hereinafter, an embodiment of the present invention will be described with reference to the drawings.

[0024] In the present application, a "donor" and a "recipient" may be a human or a non-human animal. That is, in the present application, an "organ" may be a human organ or a non-human animal organ. Furthermore, the non-human animal may be a rodent including a mouse and a rat, an ungulate including a pig, a goat, and a sheep, a non-human primate including a chimpanzee, or another non-human mammal, or may be an animal other than a mammal.

[0025] 1 is a diagram showing the piping configuration of a perfusion apparatus 1 according to one embodiment. This perfusion apparatus 1 is an apparatus for temporarily preserving an organ 9 excised from a donor outside the body until transplantation into a recipient during organ transplant surgery. The perfusion apparatus 1 preserves the organ 9 while supplying a perfusion fluid to the blood vessels within the organ 9.

[0026] In this embodiment, the case where the organ 9 to be preserved is the liver will be described. However, the organ 9 preserved in the perfusion apparatus 1 may be other organs such as the kidney, heart, or pancreas, or may be a part of an organ.

[0027] As shown in FIG. 1, the perfusion apparatus 1 of this embodiment includes a reservoir 10, a reactor 20, a perfusion fluid supply line 30, a perfusion fluid recovery line 40, a control unit 50, and a display 60.

[0028] The reservoir 10 is a container that stores perfusion fluid. For example, a pouch bag (a so-called infusion bag) that stores perfusion fluid is used as the reservoir 10. The reservoir 10 is replaced with a new one after each use. However, the reservoir 10 may be a container other than a pouch bag (for example, a bottle). Typically, ETK solution or UW solution is used as the perfusion fluid supplied from the reservoir 10. However, perfusion fluids other than ETK solution and UW solution may also be used.

[0029] As shown in Fig. 1, the perfusion device 1 includes a fluid volume sensor 11. The fluid volume sensor 11 is attached to the reservoir 10. The fluid volume sensor 11 detects whether the amount of perfusion fluid stored in the reservoir 10 is equal to or greater than a predetermined amount. The fluid volume sensor 11 is also electrically connected to the control unit 50. The fluid volume sensor 11 outputs a detection signal to the control unit 50 indicating whether the amount of perfusion fluid stored in the reservoir 10 is equal to or greater than a predetermined amount.

[0030] The perfusion device 1 may also include a sensor that detects the pH value of the perfusion fluid stored in the reservoir 10. The perfusion device 1 may also include a sensor that detects the oxygen concentration of the perfusion fluid stored in the reservoir 10.

[0031] The reactor 20 is a container that contains the organ 9. The reactor 20 is placed below the reservoir 10. A perfusion fluid is stored in the reactor 20, and the organ 9 is immersed in the perfusion fluid. In the reactor 20, the organ 9 may be cooled with ice or the like. It is desirable that the organ 9 in the reactor 20 be maintained at a low temperature of, for example, about 4°C. In the example of FIG. 1 , the reactor 20 is placed in an enclosed space in a chamber 21.

[0032] The perfusion fluid supply line 30 is a piping system for supplying perfusion fluid from the reservoir 10 to the organ 9 in the reactor 20. When the organ 9 is a liver, the perfusion fluid is supplied to the portal vein and hepatic artery of the liver. For this reason, as shown in FIG. 1 , the perfusion fluid supply line 30 has two supply pipes 31. The supply pipes 31 are elastically deformable tubes. The upstream end of each supply pipe 31 is connected to an output port of the reservoir 10. The downstream end of one supply pipe 31 is connected to the portal vein of the liver via a cannula. The downstream end of the other supply pipe 31 is connected to the hepatic artery of the liver via a cannula.

[0033] Each supply pipe 31 is provided with a pinch valve 32. The pinch valve 32 is electrically connected to the control unit 50. The pinch valve 32 closes or opens the flow path in the supply pipe 31 in accordance with a control signal supplied from the control unit 50. The reservoir 10 is disposed above the reactor 20. Therefore, when the pinch valve 32 is opened, the head difference between the reservoir 10 and the organ 9 causes the perfusion fluid in the reservoir 10 to flow through the supply pipe 31 into the organ 9.

[0034] Each supply pipe 31 is also provided with a bubble trap 33 and a sensor unit 34. The bubble trap 33 is provided upstream of the sensor unit 34. The bubble trap 33 removes air bubbles from the perfusion fluid flowing through the supply pipe 31. The sensor unit 34 is electrically connected to the control unit 50. The sensor unit 34 detects the flow rate, pressure, temperature, and presence or absence of air bubbles of the perfusion fluid flowing through the supply pipe 31, and outputs a signal indicating the detection result to the control unit 50.

[0035] As described above, in this perfusion device 1, the perfusion fluid is supplied from the reservoir 10 to the organ 9 by using the head difference. For this reason, the supply pipe 31 is not provided with a pump that forcibly pumps the perfusion fluid. By using the head difference, the burden on the organ 9 can be reduced compared to pumping by a pump. In particular, if the flow path resistance of the blood vessels in the organ 9 is high, pumping by a pump may place excessive pressure on the organ 9. However, by using the head difference, the perfusion fluid can be supplied without placing excessive pressure on the organ 9.

[0036] The perfusate recovery line 40 is a piping system for recovering perfusion fluid discharged from the organ 9 in the reactor 20. When the organ 9 is the liver, the perfusion fluid is discharged into the reactor 20 from the hepatic vein of the liver. The perfusion fluid recovery line 40 returns the perfusion fluid stored in the reactor 20 to the reservoir 10. As shown in FIG. 1 , the perfusion fluid recovery line 40 has a recovery pipe 41 connecting the reactor 20 and the reservoir 10. The recovery pipe 41 is an elastically deformable tube. The upstream end of the recovery pipe 41 is connected to the perfusion fluid in the reactor 20. The downstream end of the recovery pipe 41 is connected to the input port of the reservoir 10.

[0037] The recovery pipe 41 is provided with a pump 42. For example, a roller pump is used as the pump 42. The pump 42 is electrically connected to the control unit 50. When the control unit 50 operates the pump 42, a liquid flow is formed in the recovery pipe 41 from the reactor 20 toward the reservoir 10. As a result, the perfusion liquid discharged from the organ 9 is supplied again to the reservoir 10 through the recovery pipe 41.

[0038] However, the recovery pipe 41 may also be configured to recover the perfusion fluid discharged from the organ 9 into a waste tank without returning it to the reservoir 10.

[0039] Furthermore, a filter unit 43 is provided in the recovery pipe 41. The filter unit 43 filters the perfusion fluid flowing through the recovery pipe 41. When foreign matter such as tissue fragments is discharged from the organ 9 together with the perfusion fluid, the foreign matter is removed by the filter unit 43.

[0040] An oxygenator 44 is also provided in the recovery pipe 41. The oxygenator 44 is located downstream of the pump 42. The oxygenator 44 supplies oxygen to the perfusion fluid flowing through the recovery pipe 41, dissolving the oxygen. For example, an oxygen exchanger using a hollow fiber membrane is used as the oxygenator 44. The oxygenator 44 also has the function of maintaining the perfusion fluid at a constant temperature using temperature-controlled water supplied from a temperature-controlled water generator (not shown). The perfusion fluid is maintained at a low temperature, for example, about 4°C, which is sufficiently lower than body temperature.

[0041] The filter unit 43 and the oxygenator 44 may be integrated into one unit.

[0042] The control unit 50 is a unit for controlling the operation of each part of the perfusion apparatus 1. Fig. 2 is a control block diagram of the perfusion apparatus 1. The control unit 50 is composed of a computer having a processor 51 such as a CPU, a memory 52 such as RAM, and a storage unit 53 such as a hard disk drive. A computer program CP for controlling the operation of the perfusion apparatus 1 is stored in the storage unit 53.

[0043] 2, the control unit 50 is electrically connected to the above-described fluid volume sensor 11, pinch valve 32, sensor unit 34, and pump 42. The control unit 50 is also electrically connected to a first holder sensor S1 and a second holder sensor S2, which will be described later. The control unit 50 controls the operation of these components in accordance with a computer program CP stored in the storage unit 53. This allows the perfusion process for the organ 9 to proceed.

[0044] The display 60 is a device that displays the operating status of the perfusion apparatus 1. The display 60 is, for example, a liquid crystal display. The display 60 displays the flow rate, pressure, temperature, etc. of the perfusion fluid on the screen. In this embodiment, the display 60 has a touch panel function that allows information to be input. A user of the perfusion apparatus 1 can input necessary information to the control unit 50 by touching the display 60 while checking the display on the display 60.

[0045] 3 is a flowchart showing the flow of transplant surgery for organ 9. When performing transplant surgery, first, the donor's abdomen is opened and the organ 9 is extracted from the donor (step ST1). Specifically, the blood vessels extending from the donor's organ 9 are cut, and the organ 9 is removed from the donor's body cavity.

[0046] The extracted organ 9 is kept immersed in the low-temperature perfusion fluid in the reactor 20 (step ST2). Next, the supply pipe 31 is connected to the organ 9. Then, the pinch valve 32 is opened to start the supply of perfusion fluid from the reservoir 10 to the organ 9. This allows the organ 9 to be perfused and preserved (step ST3).

[0047] When the organ 9 is in a so-called warm ischemic state in which blood flow is cut off while the organ 9 is at room temperature, the organ 9 is prone to deterioration due to metabolism within the organ 9. Therefore, in step ST3, the organ 9 is stored at a temperature lower than room temperature to suppress deterioration of the organ 9.

[0048] Thereafter, the recipient's abdomen is opened and the organ 9 is placed in the recipient's body cavity (step ST4).Then, the supply pipe 31 is removed from the organ 9, and the blood vessels of the organ 9 are anastomosed to the blood vessels of the recipient (step ST5).

[0049] 2. Physical Structure of the Perfusion Device Next, the physical structure of the perfusion device 1 will be described. Figures 4 and 5 are views of the perfusion device 1 as seen from the front. Figure 4 shows the perfusion device 1 without the reservoir 10, perfusate supply line 30, and perfusate recovery line 40 attached. Figure 5 shows the perfusion device 1 with the reservoir 10, perfusate supply line 30, and perfusate recovery line 40 attached.

[0050] 4 and 5, the perfusion device 1 includes a main body case 70 in addition to the components shown in Fig. 1. The main body case 70 is a housing that holds the reservoir 10, the perfusion fluid supply line 30, the perfusion fluid recovery line 40, the control unit 50, and the display 60. A plurality of wheels 71 are provided on the bottom of the main body case 70. These wheels 71 allow the main body case 70 to be moved relative to the floor.

[0051] The main body case 70 has a generally rectangular parallelepiped shape with a height greater than the left-right and depth dimensions. The front of the main body case 70 is provided with a mounting surface 72 for attaching the perfusion fluid supply line 30 and the perfusion fluid recovery line 40. In this embodiment, the mounting surface 72 is inclined so that its normal is slightly upward rather than horizontally facing forward. This makes it easier for the user to perform tasks on the mounting surface 72.

[0052] When using the perfusion device 1, the user attaches the perfusion fluid supply line 30 and the perfusion fluid return line 40 to the mounting surface 72 of the main body case 70. That is, the user attaches the perfusion fluid supply line 30 and the perfusion fluid return line 40 to the front surface of the main body case 70, rather than inside the main body case 70. This makes it easier to attach and detach the perfusion fluid supply line 30 and the perfusion fluid return line 40 to and from the main body case 70 than when the perfusion fluid supply line 30 and the perfusion fluid return line 40 are arranged inside the main body case 70.

[0053] As shown in Fig. 4, two first guide lines L1 and one second guide line L2 are provided on the placement surface 72 of the main body case 70. The first guide line L1 is a line indicating the position where the supply pipe 31 should be placed. The second guide line L2 is a line indicating the position where the recovery pipe 41 should be placed. The first guide line L1 and the second guide line L2 are formed on the placement surface 72 using paint or an adhesive sheet, for example. However, the first guide line L1 and the second guide line L2 may also be lines formed by grooves or protrusions formed on the placement surface 72.

[0054] The user places the supply pipe 31 along the first guideline L1. The user also places the recovery pipe 41 along the second guideline L2. This makes it easy to place the supply pipe 31 and the recovery pipe 41 in the correct positions.

[0055] The first guideline L1 and the second guideline L2 preferably have different line shapes. For example, the first guideline L1 and the second guideline L2 preferably have different colors. This makes it easy to distinguish between the position where the supply pipe 31 should be placed and the position where the recovery pipe 41 should be placed. This prevents the supply pipe 31 and the recovery pipe 41 from being confused, and makes it easier to place the supply pipe 31 and the recovery pipe 41 in the correct positions.

[0056] It is also desirable that the two first guide lines L1 are different colors, which makes it easy to distinguish between the position where one supply pipe 31 should be placed and the position where the other supply pipe 31 should be placed. This prevents the two supply pipes 31 from being mixed up, and makes it easier to place each supply pipe 31 in the correct position.

[0057] Furthermore, two first holders H1 and one second holder H2 are provided on the placement surface 72 of the main body case 70. The first holder H1 holds a portion of the supply pipe 31. The second holder H2 holds a portion of the recovery pipe 41. The first holder H1 also has a first holder sensor S1. The first holder sensor S1 detects whether the supply pipe 31 is being held by the first holder H1. The second holder H2 also has a second holder sensor S2. The second holder sensor S2 detects whether the recovery pipe 41 is being held by the second holder H2.

[0058] Furthermore, the mounting surface 72 of the main body case 70 may be provided with holders for attaching the pinch valve 32, the bubble trap 33, the sensor unit 34, the pump 42, the filter unit 43, the oxygenator 44, and the like.

[0059] The control unit 50 and the electrical wiring connected to the control unit 50 are housed inside the main body case 70. This prevents droplets of perfusion fluid or the like from getting on the control unit 50 and the electrical wiring, thereby reducing the risk of breakdowns in the control unit 50 and the electrical wiring.

[0060] The display 60 is disposed on the upper part of the main body case 70. This allows the width of the perfusion device 1 to be reduced compared to when the display 60 is disposed on the side of the main body case 70. Also, the depth of the perfusion device 1 can be reduced compared to when the display 60 is disposed on the rear of the main body case 70. Furthermore, a lamp such as an LED that emits light in response to various alerts may be further provided on the upper part of the main body case 70.

[0061] 4 and 5, the perfusion device 1 also has a reservoir frame 73 that suspends the reservoir 10. The reservoir frame 73 has a shaft that extends upward from the top surface of the main body case 70 and an arm that extends laterally from the upper end of the shaft. The upper end of the reservoir 10 is attached to the arm.

[0062] In this way, the reservoir 10 is also disposed on the upper part of the main body case 70. This allows the width of the perfusion device 1 to be reduced compared to when the reservoir 10 is disposed on the side of the main body case 70. Also, the depth of the perfusion device 1 can be reduced compared to when the reservoir 10 is disposed behind the main body case 70.

[0063] The perfusion device 1 also has an elevator mechanism 74 that raises and lowers the reservoir frame 73. The elevator mechanism 74 is provided on the top surface of the main body case 70. The elevator mechanism 74 can fix the reservoir frame 73 at any height. The elevator mechanism 74 can also release the fixation of the reservoir frame 73 to change the height of the reservoir frame 73. This allows the height of the reservoir 10 to be changed together with the reservoir frame 73. This makes it possible to adjust the head difference between the reservoir 10 and the organ 9. As a result, the pressure of the perfusion fluid supplied to the organ 9 can be adjusted.

[0064] The user of the perfusion device 1 can change the height of the reservoir 10 by operating the lifting mechanism 74 in accordance with the flow rate or pressure of the perfusion fluid detected by the sensor unit 34. Specifically, if the flow rate or pressure of the perfusion fluid is lower than a predetermined value, the height of the reservoir 10 is raised, and if the flow rate or pressure of the perfusion fluid is higher than the predetermined value, the height of the reservoir 10 is lowered. This allows the flow rate or pressure of the perfusion fluid supplied to the organ 9 to be maintained approximately constant.

[0065] The control unit 50 closes the pinch valve 32 when the fluid volume sensor 11 detects that the amount of perfusion fluid in the reservoir 10 is less than a predetermined value. Therefore, when the amount of perfusion fluid in the reservoir 10 falls below the predetermined value, the supply of perfusion fluid to the organ 9 is automatically stopped. This makes it possible to prevent air bubbles from being supplied to the organ 9. As a result, the safety of the perfusion process can be improved.

[0066] In the state shown in Fig. 5, the chamber 21 is disposed in front of the main body case 70. On the other hand, in the state shown in Fig. 4, the chamber 21 is not disposed in front of the main body case 70. As described above, in the perfusion apparatus 1 of this embodiment, the chamber 21 including the reactor 20 is detachable from the main body case 70. Therefore, immediately after the organ 9 is extracted from the donor or immediately before transplantation of the organ 9 into the recipient, the organ 9 can be held in the reactor 20 in the chamber 21 detached from the main body case 70. This further improves the preservation state of the organ 9.

[0067] 3. Modifications Although one embodiment of the present invention has been described above, the present invention is not limited to the above embodiment.

[0068] In the above embodiment, a case has been described in which so-called low-temperature perfusion is performed, in which a perfusion fluid is supplied while maintaining an organ at a low temperature of about 4° C. However, the perfusion device of the present invention is not limited to a device that performs low-temperature perfusion, and may also be a device that supplies a perfusion fluid while maintaining an organ at a temperature equivalent to body temperature.

[0069] In the above embodiment, the user changes the height of the reservoir 10 by operating the lifting mechanism 74. However, the lifting mechanism 74 may change the height of the reservoir 10 using a power source such as a motor or an air cylinder. In this case, the control unit 50 may change the height of the reservoir 10 by controlling the lifting mechanism 74 in accordance with the flow rate or pressure of the perfusion fluid detected by the sensor unit 34.

[0070] In the above embodiment, the organ 9 to be preserved is a liver. Therefore, the perfusion apparatus 1 in the above embodiment is provided with two perfusate supply lines 30 and one perfusate return line 40. However, the number of perfusate supply lines 30 and perfusate return lines 40 may be selected appropriately depending on the type of organ 9.

[0071] Furthermore, the elements appearing in the above-described embodiments and modifications may be combined as appropriate within the scope of not causing any contradiction.

[0072] 1: Perfusion device 9: Organ 10: Reservoir 11: Fluid volume sensor 20: Reactor 21: Chamber 30: Perfusate supply line 31: Supply piping 32: Pinch valve 33: Bubble trap 34: Sensor unit 40: Perfusate recovery line 41: Recovery piping 42: Pump 43: Filter unit 44: Oxygenator 50: Control unit 60: Display 70: Main body case 72: Mounting surface 73: Reservoir frame 74: Lifting mechanism H1: First holder H2: Second holder L1: First guideline L2: Second guideline S1: First holder sensor S2: Second holder sensor

Claims

1. A perfusion device for supplying perfusion fluid to an organ, comprising: a reservoir for storing perfusion fluid; a reactor arranged below the reservoir and accommodating the organ; a supply pipe for supplying perfusion fluid from the reservoir to the organ in the reactor by means of a head difference; a main body case to which the supply pipe is attached; and a display for displaying the operating status of the perfusion device, wherein the supply pipe is attached to the front of the main body case, and the reservoir and the display are arranged on top of the main body case.

2. A perfusion device according to claim 1, further comprising a collection pipe for collecting perfusion fluid discharged from an organ, said collection pipe being attached to the front surface of said main body case.

3. A perfusion device according to claim 2, wherein the main body case has, on its front surface, a first guideline indicating the position where the supply pipe should be placed, and a second guideline indicating the position where the recovery pipe should be placed.

4. A perfusion device according to claim 3, wherein the first guideline and the second guideline are different colors.

5. A perfusion device according to any one of claims 2 to 4, wherein the main body case has: a first holder that holds the supply piping; a first holder sensor that detects whether the supply piping is held by the first holder; a second holder that holds the recovery piping; and a second holder sensor that detects whether the supply piping is held by the second holder.

6. A perfusion device according to any one of claims 1 to 5, further comprising an elevation mechanism for changing the height of the reservoir.

7. A perfusion device according to any one of claims 1 to 6, further comprising: a fluid volume sensor that detects whether the amount of perfusion fluid in the reservoir is equal to or greater than a predetermined amount; a valve provided in the supply pipe; and a control unit that closes the valve when the fluid volume sensor detects that the amount of perfusion fluid in the reservoir is less than the predetermined amount.

8. A perfusion device according to any one of claims 1 to 7, wherein the reactor is separable from the main body case.

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