Plasma adsorption filter and use thereof

Abstract

Some embodiments of the present application provide a plasma adsorption filter and use thereof. According to the embodiments of the present application, the plasma adsorption filter comprises a filter and an adsorber. The filter and the adsorber are laid flat and connected by means of adhesive bonding. The filter is a polyethersulfone membrane, and the adsorber is an aldocellulose membrane. The thickness of the polyethersulfone membrane is 25-30 μm, and the thickness of the aldocellulose membrane is 0.01-100 μm. The aldocellulose membrane is a dialdehyde-modified cellulose membrane.

Description

Plasma adsorption filter and application

[0001] CROSS-REFERENCE TO RELATED APPLICATIONS

[0002] This application is related to the Chinese Patent Application No. 202411223641.3 entitled "Plasma adsorption filter and application" filed on September 03, 2024, which is incorporated by reference in its entirety into this application TECHNICAL FIELD

[0003] The present application relates to the technical field of membrane materials, in particular to a plasma adsorption filter and application. BACKGROUND

[0004] Plasma adsorption filter is a medical device used for blood purification, mainly used for treating various diseases caused by accumulation of toxins, inflammatory mediators or metabolic products in the body. Its working principle is to remove these harmful substances from the plasma through adsorption technology to achieve the purpose of purifying blood. It can be applied to remove toxic substances such as bilirubin and ammonia in blood, relieve the symptoms of liver failure. It can also reduce inflammation by removing bacterial toxins and inflammatory mediators, and improve patient prognosis.

[0005] Interleukin 8 (IL-8) is a chemokine mainly secreted by macrophages and epithelial cells. IL-8 plays an important role in the immune system, especially in attracting and activating neutrophils. It has many biological functions, including chemotaxis: IL-8 is a potent chemokine that can attract neutrophils, T lymphocytes and eosinophils to the site of inflammation. Activation: IL-8 can activate neutrophils, enhance their phagocytic ability and bactericidal activity. Inflammatory response: IL-8 plays a key role in the inflammatory response, and excessive IL-8 can lead to chronic inflammation and tissue damage.

[0006] In some clinical treatment programs, plasma adsorption filter may be used to regulate the level of inflammatory mediators such as interleukin 8 to control the inflammatory response. For example, in severe sepsis or autoimmune diseases, removing excessive IL-8 through plasma adsorption filter may help alleviate symptoms and improve the condition.

[0007] The inventors have found that the prior art at least has the following problems: the existing plasma adsorption filter device needs to be improved in the application of this technical development. SUMMARY

[0008] The purpose of some embodiments of the present application is to provide a plasma adsorption filter.

[0009] The plasma adsorption filter comprises a filter and an adsorber, which are connected by adhesive connection, the filter is a polyether sulfone membrane, and the adsorber is an aldehyde-based cellulose membrane; the thickness of the polyether sulfone membrane is 25-30 μm, and the thickness of the aldehyde-based cellulose membrane is 0.01-100 μm.

[0010] The thickness of the polyether sulfone membrane is 25-30 μm, for example, 25 μm, 26 μm, 27 μm, 28 μm, 29 μm or 30 μm, etc.

[0011] In addition, the aldehyde-based cellulose membrane is a dialdehyde-modified cellulose membrane.

[0012] In the present application, the more aldehyde groups on the cellulose backbone, the better the adsorption effect on interleukin-8. Moreover, the whole adsorption process is relatively mild.

[0013] In addition, the dialdehyde-modified cellulose membrane is prepared by the following method:

[0014] Using cellulose as the base material, sodium periodate is used for oxidation reaction to obtain a dialdehyde-modified cellulose membrane.

[0015] In the reaction process of the present application, the selective oxidation reaction of the secondary carbon hydroxyl groups at the C2 and C3 positions under the action of sodium periodate causes the ring opening of the cellulose structural unit to generate dialdehyde cellulose.

[0016] The thickness of the aldehyde-based cellulose membrane is 0.01-100 μm, for example, 0.01 μm, 1 μm, 10 μm, 20 μm, 40 μm, 60 μm, 80 μm or 100 μm, etc.

[0017] In the present application, the thickness of the aldehyde-based cellulose membrane affects the adsorption filtration effect. The main reason is due to the principle of depth filtration. When the thickness is thicker, the more functional groups the plasma passes through the membrane and responds, and the better the adsorption effect. When the thickness is thinner, the filtration is relatively less, and the adsorption effect is reduced. In the present application, the thickness of the aldehyde-based cellulose membrane is in the above range, and the effect is best. When the thickness of the membrane is too large, the transmembrane pressure difference will increase, affecting the filtration speed.

[0018] In addition, the number of layers of the filter is 1-3 layers, for example, 1 layer, 2 layers, 3 layers, etc.

[0019] In addition, the number of layers of the adsorber is 1-3 layers, for example, 1 layer, 2 layers, 3 layers, etc.

[0020] In addition, the filter and the adsorber are alternately connected by adhesive connection, or sequentially connected by adhesive connection.

[0021] In the present application, the meaning of alternately bonding connection is that the filter, adsorber, filter, adsorber are alternately bonded; the meaning of sequentially bonding connection is that the filter, filter, adsorber, adsorber are sequentially bonded.

[0022] The present application further provides an application of the plasma adsorption filter in adsorbing interleukin-8 in the plasma. DETAILED DESCRIPTION

[0023] In order to make the purpose, technical scheme and advantages of the present application more clear, the following further describes the embodiments of the present application in combination with the embodiments. It should be understood that the specific embodiments described herein are only used to explain the present application, and are not used to limit the present application.

[0024] The endpoints of the ranges and any values disclosed herein are not limited to the precise values stated. The endpoints of the ranges and the values are approximate values and should be understood to include values approximately close thereto. For ranges, the endpoints are included between each respective range; the endpoints are included in the respective range and between the individual points; and the individual points are included in the range. It is intended to cover all ranges, closed and open, of any value or values specified herein.

[0025] The first embodiment of the present application relates to a plasma adsorption filter.

[0026] The structure of the plasma adsorption filter is sequentially arranged in four layers of filter, adsorber, filter and adsorber, and the filter is a 29 μm thick polyether sulfone membrane, and the adsorber is a 60 nm dialdehyde modified cellulose membrane.

[0027] The second embodiment of the present application relates to a plasma adsorption filter.

[0028] The structure of the plasma adsorption filter is sequentially arranged in three layers of filter, adsorber and filter, and the filter is a 26 μm thick polyether sulfone membrane, and the adsorber is a 40 μm dialdehyde modified cellulose membrane.

[0029] The third embodiment of the present application relates to a plasma adsorption filter.

[0030] The structure of the plasma adsorption filter is sequentially arranged in three layers of adsorber, filter and adsorber, and the filter is a 28 μm thick polyether sulfone membrane, and the adsorber is a 80 μm dialdehyde modified cellulose membrane.

[0031] The fourth embodiment of the present application relates to a plasma adsorption filter.

[0032] The structure of the plasma adsorption filter is sequentially arranged in four layers of filter, filter, adsorber and adsorber, and the filter is a 30 μm thick polyether sulfone membrane, and the adsorber is a 90 μm dialdehyde modified cellulose membrane.

[0033] The fifth embodiment of the present application relates to a plasma adsorption filter.

[0034] The plasma adsorption filter is the same as the first embodiment, only the dialdehyde-modified cellulose membrane used is different. The aldehyde group content of the dialdehyde-modified cellulose membrane is reduced by half. That is, during the preparation process, the amount of sodium periodate is reduced by half.

[0035] Compared with the prior art, in the embodiments provided by the present application, the chemical adsorption of interleukin can be achieved through the Schiff base chemical reaction between the aldehyde group on the membrane and the amino group of interleukin, and the adsorption process is not affected by interfering ions. At the same time, the cellulose material itself has the characteristics of green degradability, low toxicity, acid and alkali resistance, and stable structure. Combined with the use of polyether sulfone membrane, the combination of the two can further improve the strength of the plasma adsorption filter, improve the biocompatibility, reduce the adverse reactions caused by blood filtration, improve the effect of plasma adsorption filtration, and especially the adsorption effect of interleukin-8.

[0036] The present application also has three comparative examples. Comparative example one only uses 1 layer of filter; comparative example two only uses 1 layer of adsorber; comparative example three differs from the first embodiment in that the adsorber is not modified with aldehyde group, but only uses ordinary cellulose membrane.

[0037] The plasma filtration and adsorption devices of the above embodiments and comparative examples are tested for performance, and the adsorption effect is tested. The specific results are shown in Table 1.

[0038] Test steps:

[0039] The plasma containing interleukin-8 (concentration of 252.1 pg / mL) is filtered once through the plasma filtration membrane (3M, 1 filter piece with a diameter of 4.7 cm) of Jiangxia Company, and the filtration speed is 1.1 mL / min; the filtration pressure is kept within 100 kPa, until the plasma becomes clear.

[0040] The plasma filtered by the Jiangxia plasma filtration membrane is used for filtration of the adsorption filter. The adsorption filter membrane has a diameter of 47 mm and is attached to the lower side of the Jiangxia filtration membrane (1 piece), and the filtration speed is 1.1 mL / min, and the plasma dosage is 10 mL.

[0041] After the plasma is filtered by the above process, the concentration of interleukin-8 in the plasma before and after filtration is tested by chemiluminescence method.

[0042] Table 1

[0043] From the data of the examples and the comparative examples of the present application, it can be seen that: (1) the content of aldehyde groups affects the adsorption effect, and the adsorption layer with high content of aldehyde groups can completely remove interleukin in the plasma; (2) the thickness of the adsorption layer is positively correlated with the adsorption amount; (3) the polysulfone membrane alone or the unmodified cellulose membrane has limited adsorption effect on interleukin.

[0044] Compared with the prior art, the plasma adsorption filter provided by the present application can efficiently remove toxins and metabolites in the plasma, enhance the selectivity and specificity of the plasma, specifically adsorb interleukin-8 in the plasma, and has good biocompatibility. In addition, the plasma adsorption filter is also easy to functionalize, and the aldehyde groups on the surface of the aldehyde cellulose membrane provide convenience for further functionalization. Through technical extension, the multifunctionalization of the membrane can be further realized by introducing other functional groups (such as amine groups, carboxyl groups, etc.), so as to adapt to different application requirements and expand its application range. Since the pore size structure of the aldehyde cellulose membrane can be adjusted through the preparation process, it can adapt to the separation requirements of molecules of different sizes. It has biodegradability and will not pollute the environment after use, in line with the concept of green environmental protection.

[0045] Those skilled in the art can understand that each of the above embodiments is a specific embodiment for implementing the present application, and in actual application, various changes can be made in form and details without departing from the spirit and scope of the present application.

Claims

1. Use of a plasma adsorption filter for adsorbing interleukin-8 in plasma, wherein, The plasma adsorption filter comprises a filter and an adsorber, the filter and the adsorber are connected by adhesion, the filter is a polyether sulfone membrane, and the adsorber is an aldehyde-based cellulose membrane; the thickness of the polyether sulfone membrane is 25-30 mu m, the thickness of the aldehyde-based cellulose membrane is 0.01-100 mu m; and the aldehyde-based cellulose membrane is a dialdehyde-modified cellulose membrane.

2. Use of a plasma adsorber filter according to claim 1 for adsorbing interleukin-8 from plasma, wherein, The dialdehyde-modified cellulose membrane is prepared by the following method: using cellulose as a base material, and performing an oxidation reaction by using sodium periodate to obtain a dialdehyde-modified cellulose membrane.

3. Use of a plasma adsorber filter according to claim 1 for adsorbing interleukin-8 in plasma, wherein, The filter has 1-3 layers.

4. Use of the plasma adsorber filter according to claim 1 for adsorbing interleukin-8 in plasma, wherein, The adsorber has 1-3 layers.

5. Use of the plasma adsorber filter according to claim 1 for adsorbing interleukin-8 in plasma, wherein, The filter and the adsorber are alternately connected by adhesion, or are sequentially connected by adhesion.

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