Devices for and methods of sample preparation for imaging target molecules

The chip with atomically flat surfaces and hydrophobic boundaries facilitates unperturbed DNA imaging, addressing matrix and manipulation issues in existing methods, improving speed and accuracy in DNA sizing.

WO2026096509A1 Publication Date: 2026-05-07EVIZIA INC
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Patent Information

Application Number
PCT/US2025/052917
Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
Priority Date
2024-11-01
Filing Date
2025-10-28
Publication Date
2026-05-07

AI Technical Summary

Technical Problem

Existing DNA sizing methods face limitations such as the need for multiple matrices, upper size limits, and manipulation steps that affect accuracy and speed, requiring internal standards and stretching processes which increase contamination risk.

Method used

A chip with an atomically flat imaging surface and hydrophobic boundaries defines bounded sample segments for DNA imaging, allowing target molecules to settle unperturbed, followed by a wash fluid removal of contaminants and controlled drying to maintain the natural state of the molecules.

Benefits of technology

This method enables efficient, rapid, and accurate DNA sizing without stretching or alignment, reducing contamination and increasing processing speed by allowing multiple samples on a single chip, enhancing the precision of atomic force microscopy.

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Abstract

Devices and methods of sample preparation for imaging target molecules via atomic force microscopy are provided. Accordingly, the devices include a sample support chip that has an atomically flat imaging surface of a support substrate. A bounded sample segment is defined on the imaging surface via a surrounding hydrophobic barrier. The bounded sample segment includes an imaging region that is substantially devoid of contaminants and a fluidically coupled collection region. The target molecule is electrostatically bonded to the imaging surface in the imaging region in a substantially unperturbed state.
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