In-situ manufacturing and application device and method for agricultural microalgae fertilizer

WO2026129604A1PCT designated stage Publication Date: 2026-06-25SHANDONG ANALYSIS AND TEST CENTER

Patent Information

Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
SHANDONG ANALYSIS AND TEST CENTER
Filing Date
2025-06-27
Publication Date
2026-06-25

AI Technical Summary

Technical Problem

The existing microalgae fertilizer preparation process is complex, transportation is inconvenient and affects fertilizer quality, and large-scale cultivation and collection face technical and economic challenges.

Method used

An in-situ manufacturing and application device for agricultural microalgae fertilizer is provided. By filling a first chamber with culture medium and microalgae, the in-situ growth and fertilization of microalgae are achieved through overflow holes and replenishment pipes. The growth of microalgae under light is utilized, and the replenishment of culture medium is controlled to achieve targeted fertilization.

Benefits of technology

It simplifies the preparation process of microalgae fertilizer, reduces transportation costs, improves fertilizer quality and fertilization effect, and realizes efficient in-situ manufacturing and application of microalgae fertilizer.

✦ Generated by Eureka AI based on patent content.

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Abstract

The present invention relates to the field of microbial fertilizer manufacturing. Disclosed are an in-situ manufacturing and application device and method for an agricultural microalgae fertilizer. The device comprises a first box, wherein the first box is filled with a culture solution and microalgae; a replenishment tube is in communication with the first box; a plurality of small overflow holes are provided in a side wall of the first box; the replenishment tube can replenish the culture solution in the first box; when the replenishment tube injects the culture solution into the first box and the liquid level of the culture solution reaches the small overflow holes, culture solution containing microalgae flows out into the soil; and when the injection of the culture solution into the first box is stopped, the microalgae remaining in the first box continue to grow.
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Description

An apparatus and method for in-situ manufacturing and application of agricultural microalgae fertilizer Technical Field

[0001] This invention relates to the field of microbial fertilizer manufacturing technology, specifically to an in-situ manufacturing and application device and method for agricultural microalgae fertilizer. Background Technology

[0002] With increasing global focus on sustainable agriculture and environmental protection, traditional chemical fertilizers are facing growing scrutiny due to their inefficient use and environmental pollution. These problems include soil acidification, water eutrophication, and greenhouse gas emissions caused by excessive fertilizer use. To address these challenges, researchers are seeking more environmentally friendly and efficient alternatives. Among these alternatives, microbial fertilizers, as an emerging agricultural input, have garnered widespread attention due to their ability to promote soil health, increase crop yield and quality, and reduce negative environmental impacts.

[0003] Microalgae, a biological resource rich in various nutrients, has been extensively studied and is increasingly being used as a microbial fertilizer in agricultural production. Microalgae bio-fertilizers, with their comprehensive nutrition, unique efficacy, and natural, pollution-free characteristics, have demonstrated their potential and advantages in modern agriculture. Microalgae bio-fertilizers contain abundant proteins, vitamins, minerals, and growth hormones, which can promote plant growth and enhance crop disease resistance. Studies have shown that microalgae fertilizers can increase soil organic matter content, improve soil structure, and enhance soil water and fertilizer retention capacity. Furthermore, microalgae fertilizers can enhance plant antioxidant capacity and stress resistance through their bioactive substances, such as polysaccharides, polyphenols, and carotenoids, thereby improving crop yield and quality.

[0004] However, existing methods for preparing microalgae fertilizers have many shortcomings. For example, they require separate microalgae cultivation plants, which not only involves complex preparation processes and high transportation costs, but also poses risks of damaging endogenous substances within microalgae cells if not stored properly, thus affecting fertilizer quality and effectiveness. Furthermore, large-scale cultivation and collection of microalgae also face technical and economic challenges. Summary of the Invention

[0005] The purpose of this invention is to provide an in-situ manufacturing and application device and method for agricultural microalgae fertilizer, which solves the problem that the existing technology requires microalgae to be cultivated in a separate factory, resulting in complicated preparation, inconvenient transportation, and reduced fertilizer quality and application effect.

[0006] To achieve the above objectives, the present invention provides the following technical solution: an in-situ manufacturing and application device for agricultural microalgae fertilizer, comprising a first box, the first box being filled with a culture medium and microalgae, a replenishment pipe connected to the first box, and a plurality of overflow holes on the side wall of the first box, wherein the replenishment pipe can replenish the culture medium into the first box, and when the culture medium is injected into the first box and the culture medium level reaches the overflow holes, the culture medium containing microalgae flows out into the soil, and when the injection of culture medium into the first box is stopped, the microalgae remaining in the first box continue to grow.

[0007] Preferably, the drain end of the replenishment tube is submerged below the surface of the culture medium in the first tank.

[0008] Preferably, the multiple overflow orifices are located at the same height.

[0009] Preferably, the outer wall of the first box is connected to an overflow drip tube, which guides the culture medium containing microalgae flowing out of the overflow hole to the plant roots and stems.

[0010] Preferably, the top of the first housing is covered with a transparent cover.

[0011] Preferably, it also includes a second chamber filled with culture medium, a vertical pipe connected to the second chamber, a horizontal pipe connected to the vertical pipe, the horizontal pipe being connected to the replenishment pipe, and a valve being provided on the vertical pipe.

[0012] Preferably, a scraper is slidably connected to the upper surface of the transparent cover, a first pull rope is connected to the scraper, and a rectangular counterweight is connected to the other end of the first pull rope;

[0013] A rectangular box is connected to the side of the first box body. The rectangular counterweight slides inside the rectangular box. A water outlet pipe is connected between the rectangular counterweight and the scraper. A diversion hole communicating with the water outlet pipe is opened in the scraper. One end of the water outlet pipe away from the scraper passes through the rectangular counterweight and communicates with the rectangular box. A protrusion is provided at the port of the rectangular box.

[0014] A water supply pipe is connected to the outlet pipe, and the water supply pipe is connected to an external high-pressure water source. A three-way valve is installed at the connection between the outlet pipe and the water supply pipe. When the outlet pipe is connected to the inner cavity of the rectangular box but not to the water supply pipe, the rectangular counterweight slides down onto the rectangular box under gravity, thereby draining the water in the rectangular box through the outlet pipe. The rectangular counterweight pulls the scraper to slide on the transparent cover. When the outlet pipe is connected to the water supply pipe but not to the diversion hole on the scraper, high-pressure water is injected into the rectangular box, causing the rectangular counterweight to move upward and reset.

[0015] Preferably, a sliding column is slidably connected inside the bottom end of the vertical tube, a first spring is connected between the bottom of the sliding column and the bottom end of the vertical tube, a miniature cylinder is connected to the upper part of the rectangular counterweight, and an air pipe is connected between the miniature cylinder and the lower space of the sliding column.

[0016] The sliding column slides at the connection between the vertical pipe and the horizontal pipe. A water pump is connected to the vertical pipe. When water flows through the vertical pipe, the sliding column slides down, the vertical pipe connects to the horizontal pipe, and the micro cylinder extends and drives the three-way valve to change direction.

[0017] Preferably, the side wall of the first box is connected to a drainage groove, and there are multiple overflow drip tubes, all of which are connected to the drainage groove and correspond one-to-one with the multiple overflow holes;

[0018] An installation plate is slidably connected inside the drainage channel, and multiple unclogging rods are connected to the installation plate. The multiple unclogging rods slide in multiple overflow drip tubes respectively, and a second spring is connected between the installation plate and the drainage channel.

[0019] A camshaft is rotatably connected inside the drainage channel. The camshaft is in contact with the mounting plate. A torsion spring is connected between the camshaft and the drainage channel. A receiving wheel is connected to the end of the camshaft. A second pull rope is connected between the receiving wheel and the scraper. A guide wheel that cooperates with the second pull rope is rotatably connected to the outer wall of the first box. When the scraper slides, the unblocking rod slides back and forth inside the overflow drip tube.

[0020] A method for in-situ manufacturing and application of agricultural microalgae fertilizer includes the following steps:

[0021] The first chamber is buried in the soil so that the overflow hole faces the crop;

[0022] Culture medium and microalgae were added to the first chamber, and the microalgae grew after being exposed to light.

[0023] According to the timing of crop nutrient demand, the supplement tube is used to inject culture medium into the first tank. When the liquid level in the first tank rises to the overflow hole, the culture medium containing microalgae flows out through the overflow hole and fertilizes the crop.

[0024] Stop adding culture medium to the tube. The liquid level in the first tank will no longer rise, and the remaining microalgae in the first tank will continue to grow, forming a new batch of microalgae fertilizer.

[0025] Repeat the above steps at the next fertilization time.

[0026] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0027] 1. This invention involves adding culture medium and microalgae to the first tank up to the height of the overflow hole. The microalgae grow under light. When fertilization is needed, the replenishment tube is controlled to add culture medium to the first tank, causing the liquid level in the first tank to rise. After the liquid level rises, the microalgae floating on the surface of the culture medium and some of the culture medium are discharged through the overflow hole to fertilize the crops. When the replenishment tube stops supplying culture medium to the first tank, the liquid level in the first tank no longer rises, and the culture medium and microalgae no longer discharge through the overflow hole. At this time, the microalgae remaining in the first tank continue to grow as raw materials for the next fertilization of the crops. Attached Figure Description

[0028] Figure 1 is a schematic diagram of the overall structure of the present invention;

[0029] Figure 2 is a structural schematic diagram of the first housing of the present invention;

[0030] Figure 3 is a schematic diagram of the structure of the supplementary tube of the present invention;

[0031] Figure 4 is a schematic diagram of the rectangular counterweight block of the present invention;

[0032] Figure 5 is a schematic diagram of the structure of the sliding column in this invention;

[0033] Figure 6 is a schematic diagram of the structure of the unblocking rod of the present invention;

[0034] Figure 7 is a growth curve of Chlorella vulgaris in the first chamber of the present invention;

[0035] Figure 8 is a graph showing the tomato fruit weight after the application of the Chlorella fertilizer of the present invention.

[0036] Figure 9 is a growth curve of *Xanthophyllaria* in the first chamber of the present invention;

[0037] Figure 10 shows the weight index of chili peppers after application of the yellow algae fertilizer of the present invention.

[0038] In the diagram: 1. First box; 11. Overflow hole; 12. Transparent cover; 2. Second box; 21. Vertical pipe; 22. Horizontal pipe; 23. Replenishment pipe; 3. Drainage channel; 31. Overflow drip tube; 32. Mounting plate; 33. Unclogging rod; 34. Second spring; 35. Camshaft; 36. Torsion spring; 37. Collection wheel; 38. Second pull rope; 39. Guide wheel; 4. Scraper; 41. First pull rope; 42. Rectangular counterweight; 43. Rectangular box; 44. Water outlet pipe; 441. Diversion hole; 45. Water replenishment pipe; 46. Three-way valve; 47. Miniature cylinder; 48. Sliding column; 481. First spring; 49. Air pipe. Detailed Implementation

[0039] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0040] Example 1, referring to Figures 1-6, describes an in-situ manufacturing and application device for agricultural microalgae fertilizer, comprising a first box 1 filled with a culture medium and microalgae, a replenishment pipe 23 connected to the first box 1, and multiple overflow holes 11 on the side wall of the first box 1. The replenishment pipe 23 can replenish the culture medium into the first box 1, and when the culture medium level reaches the overflow hole 11, the culture medium containing microalgae flows out into the soil. When the replenishment pipe 23 injects the culture medium into the first box 1, the culture medium containing microalgae flows out into the soil. When the injection of culture medium into the first box 1 is stopped, the microalgae remaining in the first box 1 continue to grow.

[0041] The working mechanism of the in-situ manufacturing and application device for agricultural microalgae fertilizer provided in this embodiment is as follows:

[0042] The first box 1 is fixed by being buried one-third of its length in the soil in an inverted trapezoidal shape. Culture medium and microalgae are added into the first box 1 up to the height of the overflow hole 11. The microalgae grow under the influence of light. When it is necessary to fertilize the crops, the replenishment pipe 23 is controlled to replenish the culture medium into the first box 1, so that the liquid level in the first box 1 rises. After the liquid level rises, the microalgae floating on the surface of the culture medium and some of the culture medium are discharged through the overflow hole 11 to fertilize the crops. The supply of culture medium to the first box 1 by the replenishment pipe 23 is stopped, so that the liquid level in the first box 1 no longer rises, and the culture medium and microalgae no longer discharge through the overflow hole 11. At this time, the microalgae remaining in the first box 1 continue to grow as raw materials for the next fertilization of the crops.

[0043] Among the optional methods in this embodiment, the more preferred one is:

[0044] The drain end of the supplement tube 23 is submerged below the surface of the culture medium inside the first chamber 1.

[0045] The replenishment tube 23 can be placed horizontally below the liquid surface of the first tank 1 or inserted vertically below the liquid surface of the first tank 1. When the replenishment tube 23 adds culture medium into the first tank 1, it will not cause significant disturbance to the microalgae on the upper part of the culture medium, thereby ensuring that a large amount of microalgae can be discharged from the overflow hole 11.

[0046] Among the optional methods in this embodiment, the more preferred one is:

[0047] Multiple overflow orifices 11 are located at the same height.

[0048] By setting multiple overflow holes 11 at the same height, multiple overflow holes 11 can simultaneously discharge culture solution containing microalgae to fertilize crops.

[0049] Among the optional methods in this embodiment, the more preferred one is:

[0050] The outer wall of the first box 1 is connected to an overflow drip pipe 31, which guides the culture medium containing microalgae flowing out of the overflow hole 11 to the plant roots and stems.

[0051] The culture solution containing microalgae flowing out of the overflow hole 11 can enter the overflow dropper 31. The overflow dropper 31 is oriented towards the roots and stems of the plant, thereby guiding the microalgae fertilizer and applying it directionally to the roots and stems of the plant.

[0052] Among the optional methods in this embodiment, the more preferred one is:

[0053] The top of the first box 1 is covered with a transparent cover plate 12.

[0054] By covering the top of the first chamber 1 with a transparent cover plate 12, debris will not fall into the first chamber 1, while ensuring light transmission so that the growth of microalgae is not affected.

[0055] Among the optional methods in this embodiment, the more preferred one is:

[0056] It also includes a second chamber 2, which is filled with culture medium. A vertical tube 21 is connected to the second chamber 2, and a horizontal tube 22 is connected to the vertical tube 21. The horizontal tube 22 is connected to the replenishment tube 23, and a valve is installed on the vertical tube 21.

[0057] The second chamber 2 is filled with tap water, and different inorganic nutrient components are added to the tap water to prepare a nutrient solution according to the different microalgae growth requirements. The valve on the vertical pipe 21 is opened, and the culture solution in the second chamber 2 flows into the first chamber 1 through the vertical pipe 21, the horizontal pipe 22 and the replenishment pipe 23.

[0058] Among the optional methods in this embodiment, the more preferred one is:

[0059] A scraper 4 is slidably connected to the upper surface of the transparent cover 12. A first pull rope 41 is connected to the scraper 4, and the other end of the first pull rope 41 is connected to a rectangular counterweight 42. A rectangular box 43 is connected to the side of the first box 1. The rectangular counterweight 42 slides inside the rectangular box 43. A water outlet pipe 44 is connected between the rectangular counterweight 42 and the scraper 4. A diversion hole 441 communicating with the water outlet pipe 44 is opened in the scraper 4. The end of the water outlet pipe 44 away from the scraper 4 passes through the rectangular counterweight 42 and communicates with the rectangular box 43. A protrusion is provided at the port of the rectangular box 43. A water supply is connected to the water outlet pipe 44. Pipe 45, the water supply pipe 45 is connected to an external high-pressure water source. A three-way valve 46 is provided at the connection between the outlet pipe 44 and the water supply pipe 45. When the outlet pipe 44 is connected to the inner cavity of the rectangular box 43 but not to the water supply pipe 45, the rectangular counterweight 42 slides down onto the rectangular box 43 under gravity, so that the water in the rectangular box 43 is discharged from the outlet pipe 44. The rectangular counterweight 42 pulls the scraper 4 to slide on the transparent cover plate 12. When the outlet pipe 44 is connected to the water supply pipe 45 but not to the diversion hole 441 on the scraper 4, the high-pressure water source is injected into the rectangular box 43 so that the rectangular counterweight 42 moves up and resets.

[0060] When culture medium is added to the first chamber 1, the three-way valve 46 operates simultaneously and cuts off the connection between the outlet pipe 44 and the replenishment pipe 45. At this time, the space inside the rectangular chamber 43 is connected to the diversion hole 441 on the scraper 4 through the outlet pipe 44. At this time, the rectangular counterweight 42 slides down inside the rectangular chamber 43 under the action of gravity. The clear water inside the rectangular chamber 43 is squeezed and flows from the outlet pipe 44 to the diversion hole 441 and is discharged onto the upper surface of the transparent cover plate 12. When the rectangular counterweight 42 moves down, the first pull rope 41 pulls the scraper 4 to slide on the transparent cover plate 12, making the transparent cover plate 12 more transparent. After the cover plate 12 is cleaned to ensure its light transmittance, allowing the microalgae to receive light and grow normally, the three-way valve 46 is activated after the culture medium is replenished, and the connection between the rectangular box 43 and the diversion hole 441 is cut off. At this time, the water supply pipe 45 is connected to the rectangular box 43 through the water outlet pipe 44, so that high-pressure water is supplied to the rectangular box 43, causing the rectangular counterweight 42 to move up to the port of the rectangular box 43 and be blocked by the protrusion at the port of the rectangular box 43. At this time, water is no longer replenished into the rectangular box 43, and the rectangular counterweight 42 no longer moves, preparing for the next cleaning of the transparent cover plate 12.

[0061] Among the optional methods in this embodiment, the more preferred one is:

[0062] A sliding column 48 is slidably connected inside the bottom end of the vertical pipe 21. A first spring 481 is connected between the bottom of the sliding column 48 and the bottom end of the vertical pipe 21. A miniature cylinder 47 is connected to the upper part of the rectangular counterweight 42. An air pipe 49 is connected between the miniature cylinder 47 and the lower space of the sliding column 48. The sliding column 48 slides at the connection between the vertical pipe 21 and the horizontal pipe 22. A water pump is connected to the vertical pipe 21. When water flows through the vertical pipe 21, the sliding column 48 slides down, the vertical pipe 21 and the horizontal pipe 22 are connected, and the miniature cylinder 47 extends and drives the three-way valve 46 to change direction.

[0063] The flow of culture medium in the vertical pipe 21 is driven by a water pump. When the culture medium flows, it first impacts the sliding column 48, causing the sliding column 48 to move downward. After the sliding column 48 moves down to the lower part of the connection between the vertical pipe 21 and the horizontal pipe 22, the culture medium can flow through the vertical pipe 21 into the horizontal pipe 22. At this time, the culture medium is replenished in the first box 1. When the sliding column 48 slides down, it presses the first spring 481, causing the air at the bottom of the sliding column 48 to be discharged into the micro cylinder 47 through the air pipe 49, causing the micro cylinder 47 to extend. When the micro cylinder 47 extends, it drives the three-way valve 46 to operate. When the water pump stops running, the pressure in the vertical pipe 21 is restored, and the first spring 481 pushes the sliding column 48 back to its original position. At this time, the air in the micro cylinder 47 flows back, thereby shortening the micro cylinder 47 and causing the three-way valve 46 to return to its initial state.

[0064] The miniature cylinder 47 and the three-way valve 46 can be connected by a gear and a rack. The rack is connected to the output end of the miniature cylinder 47, and the gear is connected to the valve stem of the three-way valve 46. This allows the extension and retraction of the miniature cylinder 47 to drive the valve stem of the three-way valve 46 to rotate. In addition, the valve core of the three-way valve 46 can also be directly connected to the output end of the miniature cylinder 47. This allows the extension and retraction of the miniature cylinder 47 to directly drive the valve core of the three-way valve 46 to move, thereby changing its opening and closing direction.

[0065] Among the optional methods in this embodiment, the more preferred one is:

[0066] The side wall of the first housing 1 is connected to a drainage channel 3, and there are multiple overflow drip tubes 31, all of which are connected to the drainage channel 3 and correspond one-to-one with multiple overflow holes 11. A mounting plate 32 is slidably connected inside the drainage channel 3, and multiple unblocking rods 33 are connected to the mounting plate 32. The multiple unblocking rods 33 slide in the multiple overflow drip tubes 31 respectively. A second spring 34 is connected between the mounting plate 32 and the drainage channel 3. A camshaft 35 is rotatably connected inside the drainage channel 3. The camshaft 35 is in contact with the mounting plate 32. A torsion spring 36 is connected between the camshaft 35 and the drainage channel 3. A receiving wheel 37 is connected to the end of the camshaft 35. A second pull rope 38 is connected between the receiving wheel 37 and the scraper 4. A guide wheel 39 that cooperates with the second pull rope 38 is rotatably connected to the outer wall of the first housing 1. When the scraper 4 slides, the unblocking rods 33 slide back and forth in the overflow drip tubes 31.

[0067] When the scraper 4 slides, it pulls the second pull rope 38, causing the collecting wheel 37 to rotate. This causes the camshaft 35 connected to the collecting wheel 37 to rotate. The side wall of the camshaft 35 pushes the mounting plate 32 back and forth. Combined with the elastic force of the second spring 34, the unblocking rod 33 can slide back and forth in the overflow drip tube 31, thus unblocking the overflow drip tube 31 and preventing it from becoming clogged. When clean water is injected into the rectangular box 43 and the rectangular counterweight 42 moves upward, the torsion spring 36 returns elastically, causing the collecting wheel 37 to collect the second pull rope 38 and pull the scraper 4 back to its original position. At the same time, it also tightens the first pull rope 41, preparing for the next unblocking of the overflow drip tube 31.

[0068] The guide wheel 39 guides the pulling direction of the second pull rope 38 and prevents the second pull rope 38 from wearing out too quickly.

[0069] A method for in-situ manufacturing and application of agricultural microalgae fertilizer includes the following steps:

[0070] The first box 1 is buried in the soil so that the overflow hole 11 faces the crop;

[0071] Culture medium and microalgae were added to the first chamber 1, and the microalgae grew after being exposed to light;

[0072] According to the timing of crop fertilizer demand, the supplement tube 23 is controlled to inject culture medium into the first box 1. When the liquid level in the first box 1 rises to the overflow hole 11, the culture medium containing microalgae flows out through the overflow hole 11 and fertilizes the crop.

[0073] Stop adding culture medium to tube 23. The liquid level in the first chamber 1 will no longer rise. Repeat the above operation at the next fertilization time.

[0074] Example 2, referring to Figures 7 and 8, is based on Example 1. Tap water is poured into the second chamber 2. Inorganic nutrients are added according to the growth requirements of Chlorella and tomatoes, and following the requirements of BG11 culture medium. The culture solution is dissolved and prepared for use. The valve is opened, and the culture solution from the second chamber 2 is injected into the first chamber 1 until the liquid level reaches the overflow hole 11. Chlorella is then introduced into the culture solution, and the Chlorella is cultured for fifteen days using natural light to allow it to proliferate and produce Chlorella active fertilizer. After fifteen days, the valve is opened, allowing the culture solution containing Chlorella to overflow and drip into the soil through the overflow hole 11. After completing the tomato fertilization process, the valve was closed, leaving some Chlorella in the first chamber 1. This Chlorella was used as the seed for the next cycle of cultivation. The above process was repeated every fifteen days. Chlorella fertilizer was regularly cultivated in situ and applied to tomatoes. The tomato planting cycle lasted for two months. The growth curve of Chlorella in the first chamber 1 after fifteen days is shown in Figure 7. It can be seen that Chlorella grows well in the microalgae fertilizer in situ cultivation device provided in this embodiment. The total fruit weight of tomatoes after regular application of Chlorella fertilizer is shown in Figure 8. It can be seen that the microalgae fertilizer in situ cultivation device provided in Example 1 has a significant effect on increasing tomato yield.

[0075] Example 3, referring to Figures 9 and 10, is based on Example 1. Tap water is poured into the second chamber 2. Inorganic nutrients are added according to the growth requirements of *Phyllostachys edulis* and peppers, and following the requirements of MCM culture medium. The mixture is dissolved and prepared as a culture solution. The valve is opened, and the culture solution from the second chamber 2 is injected into the first chamber 1 until the liquid level reaches the overflow hole 11. *Phyllostachys edulis* is then introduced into the culture solution. The *Phyllostachys edulis* is cultured for fifteen days using natural light to allow it to proliferate and produce active fertilizer. After fifteen days, the valve is opened, allowing the culture solution containing *Phyllostachys edulis* to overflow and drip into the soil through the overflow hole 11. After completing the fertilization process for the chili peppers, the valve was closed, leaving some yellow algae in the first chamber 1. This portion of yellow algae was used as the algae seed for the next cycle of cultivation. The above process was repeated every fifteen days. The in-situ cultivation of yellow algae fertilizer and the application of chili peppers were carried out regularly. The chili pepper planting cycle lasted for two months. The growth curve of yellow algae in the first chamber 1 after fifteen days is shown in Figure 9. It can be seen that the yellow algae grows well in the microalgae fertilizer in-situ cultivation device provided in this embodiment. The total fruit weight index of the chili peppers after regular application of yellow algae fertilizer is shown in Figure 10. It can be seen that the microalgae fertilizer in-situ cultivation device provided in Example 1 has a significant effect on increasing the yield of chili peppers.

[0076] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. An in-situ manufacturing and application device for agricultural microalgae fertilizer, characterized in that: The system includes a first box (1) filled with culture medium and microalgae. A replenishment tube (23) is connected to the first box (1). Multiple overflow holes (11) are provided on the side wall of the first box (1). The replenishment tube (23) can replenish culture medium into the first box (1). When the culture medium is injected into the first box (1) by the replenishment tube (23) and the culture medium level reaches the overflow hole (11), the culture medium containing microalgae flows out into the soil. When the injection of culture medium into the first box (1) is stopped, the microalgae remaining in the first box (1) continue to grow.

2. The in-situ manufacturing and application device for agricultural microalgae fertilizer according to claim 1, characterized in that: The drain end of the supplement tube (23) is submerged below the surface of the culture medium in the first box (1).

3. The in-situ manufacturing and application device for agricultural microalgae fertilizer according to claim 1, characterized in that: Multiple overflow orifices (11) are located at the same height.

4. The in-situ manufacturing and application device for agricultural microalgae fertilizer according to claim 1, characterized in that: The outer wall of the first box (1) is connected to an overflow drip tube (31), which guides the culture medium containing microalgae flowing out of the overflow hole (11) to the plant roots and stems.

5. The in-situ manufacturing and application device for agricultural microalgae fertilizer according to claim 4, characterized in that: The top of the first box (1) is covered with a transparent cover plate (12).

6. The in-situ manufacturing and application device for agricultural microalgae fertilizer according to claim 5, characterized in that: It also includes a second box (2), which is filled with culture medium. A vertical pipe (21) is connected to the second box (2), and a horizontal pipe (22) is connected to the vertical pipe (21). The horizontal pipe (22) is connected to the supplementary pipe (23), and a valve is provided on the vertical pipe (21).

7. The in-situ manufacturing and application device for agricultural microalgae fertilizer according to claim 6, characterized in that: A scraper (4) is slidably connected to the upper surface of the transparent cover plate (12), and a first pull rope (41) is connected to the scraper (4). The other end of the first pull rope (41) is connected to a rectangular counterweight (42). A rectangular box (43) is connected to the side of the first box (1). The rectangular counterweight (42) slides inside the rectangular box (43). A water outlet pipe (44) is connected between the rectangular counterweight (42) and the scraper (4). A diversion hole (441) communicating with the water outlet pipe (44) is opened in the scraper (4). One end of the water outlet pipe (44) away from the scraper (4) passes through the rectangular counterweight (42) and communicates with the rectangular box (43). The port of the rectangular box (43) is provided with a protrusion. The outlet pipe (44) is connected to a water supply pipe (45), which is connected to an external high-pressure water source. A three-way valve (46) is provided at the connection between the outlet pipe (44) and the water supply pipe (45). When the outlet pipe (44) is connected to the inner cavity of the rectangular box (43) but not to the water supply pipe (45), the rectangular counterweight (42) slides down onto the rectangular box (43) under gravity, so that the water in the rectangular box (43) is discharged from the outlet pipe (44), and the rectangular counterweight (42) pulls the scraper (4) to slide on the transparent cover plate (12). When the outlet pipe (44) is connected to the water supply pipe (45) but not to the diversion hole (441) on the scraper (4), the high-pressure water source is injected into the rectangular box (43) so that the rectangular counterweight (42) moves up and resets.

8. The in-situ manufacturing and application device for agricultural microalgae fertilizer according to claim 7, characterized in that: The bottom end of the vertical tube (21) is slidably connected to a sliding column (48), and a first spring (481) is connected between the bottom of the sliding column (48) and the bottom end of the vertical tube (21). The upper part of the rectangular counterweight (42) is connected to a miniature cylinder (47), and an air pipe (49) is connected between the miniature cylinder (47) and the lower space of the sliding column (48). The sliding column (48) slides at the connection between the vertical pipe (21) and the horizontal pipe (22). A water pump is connected to the vertical pipe (21). When water flows through the vertical pipe (21), the sliding column (48) slides down, the vertical pipe (21) and the horizontal pipe (22) are connected, and the micro cylinder (47) extends and drives the three-way valve (46) to change direction.

9. The in-situ manufacturing and application device for agricultural microalgae fertilizer according to claim 8, characterized in that: The side wall of the first box (1) is connected to a drainage groove (3), and there are multiple overflow drip tubes (31), and the multiple overflow drip tubes (31) are all connected to the drainage groove (3) and correspond one-to-one with the multiple overflow holes (11); An installation plate (32) is slidably connected inside the drainage channel (3). Multiple unblocking rods (33) are connected to the installation plate (32). The multiple unblocking rods (33) slide in multiple overflow drip tubes (31). A second spring (34) is connected between the installation plate (32) and the drainage channel (3). A camshaft (35) is rotatably connected inside the drainage channel (3). The camshaft (35) is in contact with the mounting plate (32). A torsion spring (36) is connected between the camshaft (35) and the drainage channel (3). A receiving wheel (37) is connected to the end of the camshaft (35). A second pull rope (38) is connected between the receiving wheel (37) and the scraper (4). A guide wheel (39) that cooperates with the second pull rope (38) is rotatably connected to the outer wall of the first box (1). When the scraper (4) slides, the unblocking rod (33) slides back and forth inside the overflow drip tube (31).

10. A method for in-situ manufacturing and application of agricultural microalgae fertilizer, applied to the in-situ manufacturing and application apparatus for agricultural microalgae fertilizer as described in claim 1, characterized in that, Includes the following steps: The first box (1) is buried in the soil so that the overflow hole (11) faces the crop; Culture medium and microalgae were added to the first box (1), and the microalgae grew after being exposed to light; According to the timing of crop fertilizer demand, control the injection tube (23) to inject culture solution into the first box (1). When the liquid level in the first box (1) rises to the position of the overflow hole (11), the culture solution containing microalgae flows out through the overflow hole (11) and fertilizes the crop. Stop replenishing the culture medium in the replenishment tube (23). The liquid level in the first box (1) will no longer rise. The remaining microalgae in the first box (1) will continue to grow and form a new batch of microalgae fertilizer. Repeat the above steps at the next fertilization time.