82 results about "Amethopterin" patented technology

The invention relates to a biological tissue material and its preparation, in particular to a calcium phosphor salt base bone repairing material and its preparation. Said material provided by the invention contains calcium phosphor salt and chitin/chitosan fiber, which existes in form of fiber thread or fiber bunch. It can also be combined with 0.1-10% chitin derivants like chitosan, phosphorylate chitin, phosphorylate chitosan, carboxylation sulation chitosan and or 0.01-0.1% collagen, bone formation protein amethopterin or adriacin. Cell experiments and animal experiments all indicate that the material has favorable biological compatibility and induction activity for osteogenesis. It can raise averagely 1 to 10 times of flexural resistant intensity of calcium phosphor base bone repairing material. The invention is a reliable in vivo degradable material for tissue repairing and treatment.

The invention discloses application of a PARP1 inhibitor in the preparation of a medicine for reversing drug resistance of tumor cells to amethopterin and belongs to the technical field of tumour biotherapy. It is found by the inventor through researches that as for malignant cells with MTX resistance and DHFR gene high-amplification, by specifically inhibiting the key protein RARP1 of A-NHEJ pathway, DHFR gene copy number can be reduce and DMs in cells can be decreased so as to reverse drug resistance of tumors and enhance efficiency of tumor therapy. Therefore, on the basis of the above researches, the invention brings forward the application of the PARP1 inhibitor in the preparation of a medicine for reversing the drug resistance of tumor cells to MTX by reducing DHFR gene copy number. The invention provides a new targeting therapeutic scheme for the MTX as the main therapeutic drug and for the biotherapy of malignant tumors which are easy to resist drugs and also provides a scientific basis for effectively fighting against MTX drug resistance. In addition, the invention is also of great positive significance for deeply understanding the nature of chemotherapy resistance and finding resistance target for individualized treatment.

An object of the present invention is to provide a hyaluronic acid-methotrexate conjugate useful as a therapeutic drug for joint disorders. There is provided a hyaluronic acid-methotrexate conjugate useful for the treatment of joint disorders, wherein methotrexate is conjugated with a hydroxy group of hyaluronic acid through a linker containing a peptide chain consisting of 1 to 8 amino acids, and the linker is conjugated with the hyaluronic acid through a carbamate group.

The invention discloses a preparation method of hyaluronic acid-alendronate sodium-methotrexate nanometer granules. The method comprises the steps of adding hyaluronic acid to an aqueous solution of EDC and NHS, after carboxyl activation, adding alendronate sodium, continuing a stirring reaction, and performing dialysis to obtain an HA-ALN coupling substance; then enabling the HA-ALN coupling substance to dissolve in DMF, adding DMSO, performing diluting to prepare an HA-ALN coupling substance solution, enabling methotrexate to dissolve in dimethylsulfoxide, and adding EDC and DMAP, to prepare a methotrexate solution; and finally, mixing the HA-ALN coupling substance solution with the methotrexate solution, and performing a normal-temperature reaction to prepare the hyaluronic acid-alendronate sodium-methotrexate nanometer granules. The prepared HA-ALN-MTX coupling substance is high in stability and favorable in dispersibility, can be targeted to cancer cells to effectively kill cancer cells, and besides, is low in toxic and side effect, and has application prospect in tumor targeted therapy.

The invention discloses a coprecipitation method for preparing a methotrexate/ layered double hydroxides (MTX/LDHs) nanocomposite material, and belongs to the technical field of materials and pharmaceutical preparations. The production method comprises the following steps that: polyethylene glycol (PEG)/water is used as a solvent, and is reacted in an environment of 5 to 20 wt% of alkali solution, and the resultant product is separated by centrifugation and then washed by centrifugation, and finally, the MTX/LDHs nanocomposite material is prepared by peptization and drying. The method has theadvantages that the MTX/LDHs nancomposite material with good monodispersity can be synthesized by adding one solvent, the operation is simple, the synthetic effect is good, and no pollution to a synthetic product exists.

The invention relates to an application of ulinastatin in the preparation of drugs for treating rheumatoid arthritis. The related drugs are derived from natural proteins which are extracted from human urine, have stable and reliable quality and few adverse reactions and can overcome the shortcomings of more side effects and the like in the traditional drugs for treating the rheumatoid arthritis. The efficacy comparison studies of rheumatoid arthritis animal models prove that the efficacy of the ulinastatin is better than an amethopterin group, and the side effects are few.

The invention discloses a culture medium for selectively separating and culturing group B streptococci and a preparation method thereof. Culture supernatant fluid of group B streptococci at the logarithmic phase and an antibiotic combination are added to a group B streptococcus selective developing basic culture medium. The culture supernatant fluid contains quorum sensing substances capable of accelerating the growth of bacteria and facilitating the speed of color development reaction and color development intensity, thereby greatly shortening the detection time; and the antibiotic combination comprises amethopterin, colistin sulfate and metronidazole, which can inhibit the growth of mixed bacterium, except the group B streptococci, thereby achieving a purpose of selectively separating and culturing the group B streptococci. When the culture medium is used for detecting the group B streptococci, the characteristics of high specificity and sensitivity, convenience in observation and the like are provided, and the culture medium can be effectively applied to rapid detection to clinic samples.

The invention relates to a preparation method of a medicine sustained-release system with pH and near-infrared light dual response. The preparation method comprises the following steps of preparing graphene oxide, preparing graphene oxide/aminated mesoporous silica, preparing methotrexate/graphene oxide/aminated mesoporous silica, and preparing a methotrexate/graphene oxide/mesoporous silica/sodium alginate medicine sustained-release system. The preparation method provided by the invention has the beneficial effects that amino groups on the surface of aminated mesoporous silica and carboxyl onsodium alginate take amidation reaction, so that amido bonds can be generated to coat the surface of the mesoporous silica with the sodium alginate, and a sealing and blocking effect is achieved on medicine methotrexate in mesopores. The amido bonds have certain pH sensitivity, and the graphene oxide has good light-to-heat conversion characteristics, so that the methotrexate/graphene oxide/mesoporous silica/sodium alginate medicine sustained-release system with pH and near-infrared light dual response is obtained. The methotrexate/graphene oxide/mesoporous silica/sodium alginate medicine sustained-release system can achieve the goal of controlled release of medicine through dual stimulation of near-infrared light and pH.

The invention discloses a yttrium-based metal organic framework material and application thereof. The chemical formula of the yttrium-based metal organic framework material (Y-MOF) is Y<4> (M)< 2>. (DMF)<3.5>. (H<2>O), and in the chemical formula, M is 2, 4, 6-tri (4-carboxyl phenyl) 1, 3, 5-triazine, 2, 4, 6-tri (4-pyridine) 1, 3, 5-triazine or 1, 3, 5-tribenzoic acid pyridine. The yttrium-basedmetal organic framework material (Y-MOF) prepared by the invention is of a porous structure, has better biocompatibility and water stability, can be used as a carrier material for simultaneously loading metal ions (Mn < 2 + >, Fe < 2 + > and the like) and drug molecules (methotrexate, busulfan, adriamycin and the like), and has the effects of magnetic resonance imaging, chemodynamic therapy and chemical drug therapy; the yttrium-based metal organic framework material (Y-MOF) is prepared by adopting a solvothermal method, so that the method is simple, convenient and safe to operate, the raw materials are easy to obtain, and the conditions are mild.

The invention discloses a methotrexate metal coordination polymer, and a preparation method and application thereof. The structural formula of the methotrexate metal coordination polymer is as described in the description, wherein R is a functional metal ion. According to the invention, GdIII and FeIII capable of being imaged by magnetic resonance imaging (MRI) are introduced into a structure of MTX to obtain a coordination polymer of MTX-GdIII and MTX-FeIII, and the polymer can be efficiently delivered to tumor cells with overexpressed folate receptors under active targeting mediation (the MTX and the folate receptor have extremely strong affinity), so that the administration dosage is reduced, and accurate and efficient diagnosis and treatment of tumor focus are realized. An MTX-ZnII coordination polymer is obtained by introducing ZnII with an immune promotion effect into the structure of the MTX, and the polymer is expected to be used for efficiently treating tumors and immune diseases.

A method for identifying a protein as being able to bind a ligand comprising, providing a molecule composed of a methotrexate moiety that is covalently bonded to the ligand; introducing the molecule into a cell which a) expresses a first fusion protein comprising a dihydrofolate reductase capable of binding methotrexate, expresses b) a second fusion protein comprising the protein, wherein one of the first and second fusion proteins also comprises a transcription activator domain and the other comprises a DNA-binding domain, and c) has a reporter gene wherein expression of the reporter gene is conditioned on the proximity of the first fusion protein to the second fusion protein; permitting the molecule to bind to the first fusion protein and to the second fusion protein so as to activate the expression of the reporter gene; and selecting the cell if it expresses the reporter gene.

The invention provides an ELISA (Enzyme-linked Immunosorbent Assay) kit for detecting methotrexate and application thereof. The kit is prepared from the following reagents: a methotrexate coated 96-pore microporous plate, a methotrexate monoclonal antibody, an antibody diluting solution, an HRP (Hypothalamic Regulatory Peptide)-labeled goat anti-mouse secondary antibody, a methotrexate standard product, a washing liquid concentrated solution, a sample diluting solution, a TMB (Tetramethylbenzidine) color-developing solution and a stopping solution. The stable, rapid and simple methotrexate detection kit is obtained through improving the combination of all reagents and improvement on parameters of the reagents; when the ELISA kit is applied, the standard deviation value of the kit is relatively small. The ELISA kit can be used for detecting the plasma drug concentration of patients who utilize the methotrexate with a large dosage and detecting the plasma drug concentration of patients who utilize the methotrexate with a small dosage.

A nimustine slow release injection for treating solid cancer comprises slow release finding and nimustine, or the combination of nimustine and relative booster (pidorubicin, osaliplatinum, adriablastina, amethopterin or the like). The viscidity of slow-release injection is 10-650cp (20-30Deg. C). The anti-cancer effective component can be made into slow release plant agent. The slow release finding substantially comprises macromolecule polymer with biological soluble degradable and absorb property, which can slow release the anti-cancer effective component to cancer part in the degradation process, significantly reduce general reaction and hold effective drug density on cancer. The anti-cancer compound can be arranged part of cancer to reduce the general toxicity reaction, selectively improve the drug density locally, and strengthen the effect of non-surgery treatments as chemotherapy, and radiation therapy or the like. The solid cancer comprises glioma, lung carcinoma, intestinal cancer, breast cancer or the like.

The invention discloses a detection kit of methotrexate metabolism markers, and a detection method and application of the detection kit. The kit is used for detecting the gene polymorphism of the methotrexate metabolism markers ABCB1 (C3435T), MTHFR (C677T) and SLCO1B1 (c.1865+4846T>C). According to the invention, multiple RPA amplification and optimized pyrosequencing technologies are combined to detect the methotrexate drug dosage and the gene polymorphism related to adverse reaction prediction, the kit can be used for simultaneously detecting ABCB1 (C3435T), MTHFR (C677T) and SLCO1B1 (c.1865+4846T>C) gene polymorphism methotrexate drugs, and suggestions from the gene perspective are provided for clinical personalized medication.

The invention discloses an artificially synthesized cationic peptide (named as AIK) and applications thereof in preparing an anti-tumor drug. The amino acid sequence of the cationic peptide is shown as SEQ ID NO.1. A research shows that the AIK has an obvious inhibition effect on various solid tumor cells, especially leukemic cells, cells of pulmonary giant cell cancer and hepatoma ascites cells, the anti-tumor activity is possibly combined an anionic acceptor on the surface of tumor cells so as to induce the tumor cells to be subjected to apoptosis and necrosis, and the anti-tumor activity is higher than that of the anti-tumor peptide molecules reported by other researchers. More importantly, the artificially synthesized 25-peptide drug molecules have higher specificity on the tumor cells. After the anti-tumor drug is locally administrated by subcutaneous tumor-bearing mice, the subcutaneous growth of liver cancer cells H22 of the tumor-bearing mice can be obviously inhibited and toxic and side effects are lower than chemotherapeutic drugs amethopterin. The artificially synthesized cationic peptide can provide theoretical basis and experimental materials for further researching and developing polypeptide anti-tumor new drugs and small-size targeted anti-tumor new drugs.

The invention belongs to the field of methotrexate synthesis, and particularly relates to a preparation method of a methotrexate compound, which comprises the following steps: under the action of a high-activity copper catalyst, synthesizing p-methylamino benzoyl L-glutamic acid zinc salt from chlorobenzoyl L-glutamic acid, methylamine and zinc chloride, and then synthesizing methotrexate from the p-methylamino benzoyl L-glutamic acid zinc salt, tetraaminopyrimidine sulfate and tribromoacetone, and the preparation method of the chlorobenzoyl L-glutamic acid is limited. According to the method, the problem of low purity of methotrexate is solved, efficient catalysis of the high-activity copper-based catalyst is utilized, and a fixed bed reaction system is matched, so that the catalytic efficiency is ensured, rapid separation of the catalyst is realized, and the stability of the product is effectively improved.

The invention discloses a transdermal drug delivery system and a preparation method and application thereof. The preparation method comprises the following steps that polydopamine composite manganese dioxide nanoparticles are provided; a PDMS female mold is prepared; and methotrexate, a high polymer material and the polydopamine composite manganese dioxide nanoparticles are mixed, a mixture is added into the PDMS female mold, drying treatment is carried out, demolding is carried out to form a microneedle patch containing methotrexate and the polydopamine composite manganese dioxide nanoparticles, and the transdermal drug delivery system is obtained. According to the transdermal drug delivery system, methotrexate and the PDA@MnO2 nanoparticles can be effectively delivered into the skin, the side effect of methotrexate can be reduced, the treatment effect of rheumatoid arthritis can further be improved by removing active oxygen through the PDA@MnO2 nanoparticles, and MRI imaging enhancement is realized through manganese ions, so that the imaging diagnosis of the rheumatoid arthritis is realized.