The invention provides a preparation technology of a giant salamander protein peptide probiotic product. The preparation technology comprises the following specific steps of adding red jujubes to giant salamander meat, performing crushing, adding water and glucamylase, performing saccharification, adding sodium selenite, performing high-temperature sterilization, performing inoculation with baker's yeast, performing aerobic fermentation, and performing filtration so as to obtain filtrate I and filter residues I; adding water, neutral protease and calcium chloride to the filter residues I, performing heat preservation at 30-40 DEG C for 2-4h, then adding kiwi fruit juice obtained through mincing and peeling, performing stirring, and performing filtration so as to obtain filtrate II and filter residues II; performing high-temperature sterilization on the fruit residues I, adding the filtrate I, performing aerobic fermentation, and performing centrifuging so as to obtain supernatant III and precipitate III; adding the filtrate II to the precipitate III, performing heat preservation at 30-40 DEG C for 2-4h, and performing filtration so as to obtain filtrate IV; and performing high-temperature sterilization on fresh milk, adding lactic acid bacteria and bifidobacteria, performing fermentation to obtain fermented milk, uniformly mixing the fermented milk with the supernatant III andthe filtrate IV, and performing storage at 0-5 DEG C.