Patents
Literature
Hiro is an intelligent assistant for R&D personnel, combined with Patent DNA, to facilitate innovative research.
Hiro

60 results about "Cell Separation Technology" patented technology

Automatic medical cell separation device

The invention belongs to the technical field of cell separation, and particularly discloses an automatic medical cell separation device which comprises a separation tube. The separation tube is internally provided with a transmission rod, the transmission rod is provided with a structure which can extrude and crush or smash a medical cell, the top end of the transmission rod is connected with a driving motor, and an outlet is arranged on the bottom part of the separation tube and is provided with a plug cover. By the medical cell separation device, a large cell tissue can be conveniently and easily extruded and crushed or smashed into cell particles, the operation process of cell separation of the separation device and an operation can be simultaneously carried out in an operating room, nonnutritive time after the medical cell separation can be effectively shortened, pollution opportunities and harms of the cell can be reduced, and the quality control of the cell treatment can be convenient; simultaneously, the storage and transportation of samples are greatly convenient, the standard operation can be carried out, and the repeatability of a research can be increased; and automation control and personalized customization can be carried out according to different tissue cells, so that the use is simple and convenient.
Owner:GUANGZHOU YIDAI PHARMA

Preparation method for improving yield of umbilical cord derived mesenchymal stem cell primary cells

The invention relates to the technical field of cell culture and cell separation and in particular discloses a preparation method for improving the yield of umbilical cord derived mesenchymal stem cell primary cells. On the basis of culturing and separating mesenchymal stem cells on a traditional Wharton's jelly tissue block in a disposable adherence manner, Wharton's jelly tissue blocks are finely collected at different time of a later period of culture and are repeatedly adhered; P0 generation mesenchymal stem cells can be continuously collected at different time. According to the method provided by the invention, the utilization rate of Wharton's jelly is maximized through a property that the mesenchymal stem cells continuously move out from Wharton's jelly tissues to grow; compared with Wharton's jelly tissue block disposable adherence culture, the obtained quantity of the P0 generation mesenchymal stem cells can be increased by two times at least; the culture density of the mesenchymal stem cells, the concentration of serum replacements in a culture medium and the culture time are subsequently controlled; when the cells are sub-cultured to a P3 generation, the obtained cell quantity can completely meet the requirements of cell storage and clinical transplantation.
Owner:北京中科易微生物科技有限公司

Separation and purification method for islet cells of mouse

InactiveCN103013907ARich optional technologyConfiguration concentration is lowVertebrate cellsArtificial cell constructsWater bathsPurification methods
The invention belongs to the technical field of cell separation, and particularly relates to a separation method for islet cells of a mouse. The method comprises the following steps: narcotizing the mouse, disinfecting the skin and cutting along the center of the abdomen to fully expose the pancreas; shearing the ligament organization connected with the pancreas of the spleen, clamping to close the junction of the splenic vein and the spleen and cutting the heart broken, inserting a needle at the intersection of the splenic vein and the portal vein to the splenic vein, and injecting collagenase filling liquid; immersing the filled pancreas of the spleen into the collagenase filling liquid after the pancreas of the spleen is dissociated from the abdominal cavity; performing water bath slaking on the pancreas collagenase solution, performing centrifugal separation after adding Hank's liquid and taking the sediment; and performing heavy-suspending sedimentation, filtration and centrifugal treatment on the sediment to obtain the islet cells of the mouse. The separation and purification method for the islet cells of the mouse, provided by the invention, is simple and feasible in operation, and the separated islet cells have no obvious differences with those obtained by a CBD method in forms and functions, so that the selectable technologies for separation of pancreas islet of the mouse are enriched, and the separative power is improved.
Owner:SHANGHAI INST FOR ENDOCRINE & METABOLIC DISEASES

Preparation method of HLA-A0201 (Human Leukocyte Antigen-A0201) restrictive anti-MAGE (MicroArray and Gene Expression) antigenic specificity CTL (Cytotoxic T Lymphocyte)

The invention discloses a preparation method of an HLA-A0201 (Human Leukocyte Antigen-A0201) restrictive anti-MAGE (Micro Array and Gene Expression) antigenic specificity CTL (Cytotoxic T Lymphocyte). The method comprises the following steps of: carrying out hemapheresis to collect peripheral blood mononuclear cells; enriching and purifying CD8+T lymphocytes; utilizing a mature dendritic cell loaded with an HLA-A0201 restrictive anti-MAGE antigen polypeptide to stimulate the CD8+T lymphocytes; utilizing rhIL-2 and rhIL-7 to accelerate the T cells to grow; utilizing a Tetramer marking method and a flow type cell separation technology to purify the target CTL; utilizing an anti-human CD3 monoclonal antibody covered by a solid phase and an IL-2 to stimulate the target CTL to grow; adding an autologous PBMC (Peripheral Blood Mononuclear Cell) radiated by gamma rays to enhance the activation of the target CTL; adding rhIL-15 to cultivate and reproduce; and collecting and identifying. The prepared target CTL has high purity, high reproduction capability, high killing activity and high ratio CTL-CM, and can be used for immune treatment of tumors and the like.
Owner:江苏得康生物科技有限公司

Cell sample extraction and subpackaging device

The invention discloses a cell sample extraction and subpackaging device, and relates to the technical field of cell separation. The cell sample extraction and subpackaging device comprises a supporting frame, wherein a centrifugal mechanism used for driving a rotating groove to rotate is in driving connection to a driving motor II; a vertically-arranged rotating column is rotationally installed on the supporting frame; and a subpackaging mechanism used for subpackaging cell samples is installed at the lower end of the rotating column. According to the cell sample extraction and subpackaging device, the arranged centrifugal mechanism can drive the rotating groove to drive a centrifugal groove to rotate, and the centrifugal separation effect of cells is achieved; an arranged suction head can convey the cells in the centrifugal groove into a storage box under the effect of a conveying mechanism, the suction head can be driven by a lifting mechanism to vertically move, the cells at different depth positions in the centrifugal groove are extracted, and cell extraction operation and adjustment are flexible and efficient; and the arranged subpackaging mechanism can stably and rapidly subpackage the extracted cells, the effect of storing and loading the cell samples on a large scale is achieved through a plurality of sets of test tubes on a rotating disc, and a large number of cell samples can be conveniently used in biological experiments.
Owner:李容尔

Separation method of macrophage in thyroid papillary carcinoma tissue

The invention provides a cell separation technology, and particularly relates to a separation method of macrophage in a thyroid papillary carcinoma tissue. The separation method comprises the following separation steps of: placing the collected thyroid papillary carcinoma tissue into D-hanks liquid; flushing; removing D-hanks liquid and connective tissues; cutting the collected thyroid papillary carcinoma tissue into blocks; adding II type collagenase into the blocked tissue; performing digestion reaction for 1 to 3 hours at temperature of 35 to 40 DEG C; adding an 1640 culture medium containing 10% of fetal calf serum for stopping the digestion reaction; filtering; centrifuging; removing supernatant; resuspending and planking cell sap through the 1640 culture medium; transferring into a cell incubator containing 5% of CO2 for incubating; fully washing through PBS (Phosphate Buffer Solution); re-culturing the obtained anchorage-dependent cells; collecting the supernatant; and freezing and storing. According to the separation method, the separated macrophage is circular and can be tightly adhered to a petri dish and shows positive by staining by CD68; the positive rate reaches more than 95%, namely, the macrophage is separated successfully.
Owner:RUIJIN HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE

Method for preparing animal mature neuron single cells

The invention discloses a method for preparing animal mature neuron single cells, and belongs to the technical field of separation of mature neuron single cells. According to the method, firstly, D-PBS is utilized for conducting perfusion on a mouse, blood cells are removed, and the influence of the blood cells on subsequent separation is avoided; Hibernate-A is utilized as a culture medium in thedigestion process, without considering the problem of early oxygen introduction or oxygen introduction in the digestion process, the time can be effectively saved by at least 30 minutes, and the damage to neurons is reduced; by means of the method, the single cells can be prepared without special instruments; through the method, oligodendrocytes, astrocytes and neurons can be simultaneously separated out, sequencing or function analysis can be conducted on different types of nerve cells in the same batch of samples, the experimental errors can be reduced, and the cost is reduced.
Owner:SHANGHAI CUTSEQ BIOMEDICAL TECH CO LTD

Separate culture method of primary tumor cells

InactiveCN109402060AEasy adherent growthAdequate growthCell dissociation methodsCulture processCancer cellHyaluronidase
The invention relates to the technical field of cell separation, in particular to a separate culture method of primary tumor cells. A composite enzyme solution is adopted for decomposing cancer tissue, wherein collagenase, trypsin and hyaluronidase in the composite enzyme solution mutually cooperate, looseness of bridging structures among the cells can be accelerated, the cancer tissue is converted to be flocculent from being blocky, thus, the cells in cell aggregate are evenly dispersed in cell suspension, and the numbers of cell alliances and single cells suspended in cell sap are increased;after inoculated culture is completed, the cells easily adhere to walls to grow, and therefore the survival rate of the cells is increased; serum extracted from the body of a patient suffering from tumors is added into a culture medium, a microenvironment most suitable for the growth of the cancer cells is provided for the cancer cells, the culture period is shortened, the culture success rate is80% or above, and the clinic consistency rate is 95% or above.
Owner:钱成穗

Cell separator with high separation efficiency

The invention discloses a cell separator with high separation efficiency. The cell separator comprises a mounting box, the top of the mounting box is slidably connected with a primary filtering mechanism, the primary filtering mechanism comprises a filtering box, the two sides of the filtering box are fixedly connected with fixing plates, the bottoms of the two fixing plates are slidably connected with the top of the mounting box through guide rails, the bottoms of the fixing plates are fixedly connected with tooth plates, the top of the mounting box is fixedly connected with first driving mechanisms, the bottom of the mounting box is fixedly connected with a filtering frame through a second driving mechanism, and the second driving mechanism comprises telescopic rods.The invention relates to the technical field of cell separation. According to the cell separator with the high separation efficiency, tissue fluid is filtered twice through the filtering box and the filtering frame, stem cells can be automatically screened out, the workload of workers is relieved, the filtering frame can move up and down in the mounting box, the filtering speed is increased, the working efficiency is improved, and the stem cell filtering and separating efficiency is greatly improved.
Owner:武汉北度生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products