28 results about "Cell Separation Technology" patented technology

The utility model discloses a cell separating and screening device and relates to the technical field of cell separation.The cell separating and screening device comprises a separating and screening box, side sliding grooves are formed in the two sides of the separating and screening box, cell preservation liquid flows out of an end through groove through the arrangement of the end through groove in an upward discharging mode, then the cell preservation liquid is gathered through a guide rod, and then the cell preservation liquid is separated and screened. The cell preservation liquid drips, the filtered cells are impacted, the gathered cells are dispersed, the cells are driven to move by utilizing the mobility of the cell preservation liquid, the screened cells can be screened out of the filtering frame, the screening effect is ensured, in this way, multiple times of screening are not needed, and the cell preservation liquid flows out from the upper portion through the design, so that the screening efficiency is improved. Separated cells can be prevented from entering a connecting pipe, waste is avoided, practicability is higher, a second driving motor is arranged to drive the connecting pipe to move, the cells are spread out, the utilization rate of the filtering frame is increased, and the filtering effect is guaranteed.

The utility model discloses a cell induced separation device, which belongs to the technical field of cell separation, and adopts the technical scheme that the cell induced separation device comprises a base, the top of the base is fixedly connected with a frame, and a first cleaning pool, a second cleaning pool and an operation table are sequentially arranged in the frame from left to right, and an ultrasonic generating device is arranged in the first cleaning pool; by arranging the flushing assembly, the flushing assembly can spray flushing fluid to the culture chamber from multiple angles, the surface of the culture chamber can be comprehensively and efficiently flushed, so that pollutants such as residual cell debris and an inducer are rapidly removed, the cleaning efficiency is greatly improved, and the cleanliness of the culture chamber is ensured; according to the cell culture device, the culture chamber can be accurately moved into the flushing assembly to be flushed, then moved into the first cleaning pool to be deeply cleaned and then moved to the operation table to be subjected to subsequent cell induction or separation operation, and the use convenience is greatly improved through the flexible position adjusting function.

The invention discloses a blood cell separation magnet device and method, and belongs to the technical field of cell separation. The magnet device comprises a plurality of pole heads which are symmetrically arranged, and runway coils are arranged outside the pole heads; the central axis position of a gap defined by the runway coils is a working area, the working area is a uniform magnetic field capturing area, and magnetized target cells in blood cells are stably separated by using a uniform magnetic field; the width of the working area ranges from 8 mm to 15 mm, and the length of the working area ranges from 200 mm to 500 mm. Through cooperation of the runway coil and the pole head, a linear or planar working area which is small in space body, high in strength, large in gradient and uniform in magnetic field distribution is formed, and compared with an existing point-shaped or small-area high gradient, magnetized cells flowing through a sample in the area can be captured more efficiently and more thoroughly, and the recovery rate and the purity are remarkably improved.

Devices and methods suitable for cell separation. The devices herein include non-random voids interconnected through non-random pores and / or non-random solid geometrical structures optionally connected through solid non-random interconnecting elements. Such devices are preferably suitable for affinity-based cell isolation techniques which rely upon binding interactions.

The invention discloses a single-cell suspension purification method capable of maintaining representativeness of a cell population and a kit, and belongs to the technical field of single-cell separation. The method comprises the following steps that a cell sample to be separated and purified is resuspended in a centrifugal container through a reagent B, then a reagent A is added and evenly mixed, the adding amount of the reagent A and the reagent B is adjusted so that the density of the suspension can be 1.08-1.15 g / ml, the reagent A is an aqueous solution containing iodixanol, and the reagent B is a DMEM culture medium containing 1-10% of FBS; and slowly laying PBS on the upper layer of the suspension, centrifuging, collecting living cells of an interface layer, washing the obtained living cells, and re-suspending. According to the scheme, the single-cell suspension for single-cell library construction can be purified under the conditions of low equipment requirement, low cost and mild conditions, the cell population representativeness of a sample can be reserved to the maximum extent while dead cells are removed, and no adverse effect is generated on the single-cell library construction process.

The invention relates to the technical field of cell separation, and particularly discloses a cell separation device which comprises a main body, the rotating platform is arranged in the main body, and the rotating platform rotates at a high speed to generate centrifugal force, so that target cells are separated from other components; the number of the bases is four, and the four bases are distributed in an annular array mode relative to the center axis of the rotating platform; the first reset spring provides upward elastic force for the sliding block, the second reset spring provides downward elastic force for the clamping seat, and when the centrifugal tube moves along with the clamping seat, the first reset spring simultaneously absorbs power of the clamping seat, protects the centrifugal tube, gently and uniformly mixes, prevents cells from settling to block a filter membrane, but avoids generation of foam; four centrifugal tubes are uniformly mixed in the rotating platform at the same time, so that the experiment process is reduced, a sample is uniformly mixed in a sterile environment, the possibility that the sample is polluted by microorganisms outside the sterile environment is reduced, and the accuracy and the treatment effect of a subsequent experiment are influenced.

The invention belongs to the technical field of cell separation, and particularly relates to a method for separating kidney immune cells, which comprises the following steps: digesting an obtained mouse kidney tissue block in an RPMI 1640 culture medium of fetal calf serum containing collagenase IV and DNAaseI, collecting an upper-layer single-cell suspension after digestion is completed, washing, centrifuging, and discarding supernate, thereby obtaining the kidney immune cells. And centrifuging by a discontinuous Percoll density gradient centrifugation method, and sucking cells of a high interface layer of 40-80% to obtain the mouse kidney immune cells. According to the technical scheme, a three-step optimization scheme of enzyme digestion, room-temperature sedimentation and density gradient separation is adopted, so that when tissues are dissociated in a warm manner, cell damage is greatly reduced, and antigen loss or degradation caused by enzyme activity and metabolism is effectively reduced.

The invention discloses a mesenchymal stem cell culture and separation device, and relates to the technical field of cell separation. Comprising a frame, a culture dish and two first telescopic pieces are arranged on a bottom plate of the frame, a second telescopic piece is arranged at the bottom of a top plate of the frame, a push plate assembly is detachably connected to the second telescopic piece, and a plurality of push plates are evenly arranged below the push plate assembly; a culture block is placed on the culture dish, and a plurality of culture cavities matched with the push plates are formed in the culture block; sliding grooves are formed in two side plates of the frame, sliding blocks are arranged in the sliding grooves in a sliding mode, the two sliding blocks are fixedly connected with the tops of the two first telescopic pieces correspondingly, and elastic columns are arranged on the sides, facing the culture dishes, of the sliding blocks; inserting holes matched with the two elastic columns are formed in the two sides of the culture block. The multi-cavity culture block is adopted to increase the adsorption area, a large number of cells are convenient to culture, meanwhile, the push plate assembly is matched to facilitate separation, and the structure is simple; the culture blocks and the push plate assembly are detachable, so that cleaning is convenient.

The invention belongs to the technical field of cell separation, and relates to a cell separation sampling instrument which is mainly composed of a shell, a centrifugal assembly and a sampling assembly, the centrifugal assembly is arranged in the shell and comprises a first telescopic rod, a second supporting rod, a third supporting rod and the like, and the third supporting rod is pushed through a hydraulic rod of the first telescopic rod; the second supporting rod and the rotating disc are linked, the centrifugal bin rotates around the square section of the rotating shaft, then the angle of the centrifugal bin is adjusted, the sampling assembly is arranged on the left side and the upper portion of the shell and can sample and collect separated blood, and during centrifugation, a test tube can be fastened, collision damage is avoided, rapid acceleration and deceleration are achieved, and the centrifugation time is shortened; meanwhile, the sampling assembly does not need to frequently replace the needle head, so that the sampling operation time is shortened, the working efficiency is integrally improved, the equipment cost is reduced, and a more efficient and practical tool is provided for cell separation research.

The utility model provides a kind of biological cell separation device, it is related to biological cell separation technical field, including separation seat, the separation seat side top edge is fixedly installed with several connection rings of the annular array distribution around separation seat center, any the coaxial distribution and fixed mounting in the base of the connection ring below separation seat side middle position is provided with, and any the circular ring and rotating seat are arranged in the position above and below of the connection ring, by being provided with several roller frame in circular ring and rotating seat, the clamping force even distribution is realized in the tube orifice and tube bottom of test tube using several roller frame resistance test tube surface, avoid test tube swing in the process of rotation, and using rotating seat rotation drives the revolution of several roller frame inside to further realize test tube rotation, the tire of roller frame in circular ring can rotate independently, when test tube rotation, the tire of roller frame in circular ring rotates under the action of friction force, further stabilize test tube tube orifice.

The invention provides a separation method and an identification method of bactrian camel testis interstitial cells, and belongs to the technical field of cell separation. The method comprises the following steps: mixing 0.08-0.12% of collagenase IV solution and 0.23-0.27% of trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) according to a volume ratio of 1: 2, digesting a bactrian camel testis tissue block, then carrying out hypotonic treatment purification to remove redundant germ cells, carrying out differential adhesion purification to remove a large amount of supporting cells, carrying out percoll density gradient centrifugation purification to remove a small amount of supporting cells, and carrying out freeze drying to obtain a finished product. And transferring the suspension with the percoll gradient of 34-60% into a new culture bottle to obtain the interstitial cells with the purity of more than or equal to 95%, and completing identification by combining morphological observation, immunofluorescence staining and secretion function detection. The separated bactrian camel testis interstitial cells can be used for double-hump reproductive performance research, and a foundation is laid for the reproductive performance of bactrian camels.

The invention provides an umbilical cord mesenchymal stem cell separation device, which belongs to the technical field of cell separation, and comprises a support table, a support frame fixedly connected to the support table, an umbilical cord cutting table fixedly connected to the top of the support frame and used for conveniently pretreating an umbilical cord, and a treatment assembly fixedly connected to the support table, a separation assembly used for separating stem cells from the umbilical cord Wharton's jelly is arranged in the treatment assembly, and a cutting assembly used for cutting up the umbilical cord Wharton's jelly is further fixedly connected to the treatment assembly. The device has excellent performance in the aspects of automation, continuity and the like of the operation process, after the Wharton's jelly separated by the cutting table is placed on the cutting table, a series of subsequent operations such as cutting, enzyme digestion, oscillation mixing and centrifugation can be automatically completed under the cooperative operation of all parts of the device, frequent manual intervention is not needed, and the working efficiency is improved. The working efficiency is greatly improved, and meanwhile, the continuity and the stability of the separation process are ensured.

The invention provides a magnetic nanoparticle mediated mesenchymal stem cell rapid separation method, and belongs to the technical field of cell separation.The magnetic nanoparticle mediated mesenchymal stem cell rapid separation method comprises the following steps that 1, a cell sample is obtained from tissue, and a cell suspension is obtained after digestion; 2) mixing the cell suspension with first magnetic nanoparticles, and performing first incubation; 3) adding second magnetic nanoparticles, and carrying out second incubation; then carrying out magnetic separation and elution to obtain mesenchymal stem cells; the first magnetic nanoparticles are modified with a CD73 antibody and a CD90 antibody; and the second magnetic nanoparticles are modified with a CD105 antibody. According to the method, through two-step magnetic nanoparticle incubation and one-step magnetic separation, the purity of the mesenchymal stem cells obtained through separation is improved on the basis of ensuring sufficient separation of the mesenchymal stem cells.

The invention discloses a device for separating cell clusters in malignant pleural effusion and a use method of the device, and belongs to the technical field of cell separation.Two clamping blocks get close to each other and are separated from a clamping groove, the locking state between a supporting base and a mounting base is relieved, then the supporting base and a plurality of centrifugal tubes can be taken out of a centrifugal machine, and when the centrifugal tubes are taken down, the centrifugal tubes are separated from the mounting base; as the central axis of the tube frame and the central axis of the supporting seat are in a parallel state, the centrifugal tubes are directly and vertically pulled out upwards, the multiple centrifugal tubes can be conveniently and synchronously disassembled, the conical seat moves downwards to extrude the two clamping blocks to be clamped into the clamping grooves, and the purpose of locking the supporting seat and the mounting seat is achieved; therefore, the purpose of rapidly assembling and disassembling the batch centrifugal tubes is achieved through the centrifugal machine, the supporting seats and the tube frames which are modularly designed, the two sets of supporting seats and the multiple tube frames are adopted for centrifugal work, the centrifugal tubes can be alternately assembled and disassembled, the time spent on assembling and disassembling the centrifugal tubes is shortened, and efficient and stable separation work on cell clusters is facilitated.

This application discloses a blood cell separation magnet device and method, belonging to the field of cell separation technology. The magnet device includes: a plurality of symmetrically arranged poles, each pole having a racetrack coil disposed outside it; the central axis of the gap formed between the racetrack coils is the working area, which is a uniform magnetic field capture area, and uses the uniform magnetic field to stably separate magnetized target cells from blood cells; the width of the working area is 8-15 mm, and the length is 200-500 mm. This application, through the cooperation of the racetrack coils and poles, forms a linear or planar working area with a very small volume, high intensity, large gradient, and uniform magnetic field distribution. Compared with existing point-like or small-area high gradients, it can more efficiently and thoroughly capture magnetized cells in samples flowing through this area, significantly improving recovery rate and purity.

The invention discloses a stem cell separation device, and belongs to the technical field of stem cell separation.The stem cell separation device comprises a base, a supporting cylinder is fixedly mounted at the top end of the base, a centrifugal cylinder is rotatably mounted in the supporting cylinder, a backflow mechanism is arranged on the outer side of the centrifugal cylinder, and the backflow mechanism comprises a first pump body mounted on the outer side of the supporting cylinder; three annular pipes are arranged between the supporting cylinder and the centrifugal cylinder and communicate with the centrifugal cylinder, the input end of the first pump body communicates with the two annular pipes arranged at the bottom end through discharging pipes, and the output end of the first pump body communicates with the annular pipe at the top end through a feeding pipe. The tissue fluid in the two annular pipes at the bottom end is absorbed through the first pump body and discharged through the annular pipe at the top end, so that a sample continuously and circularly flows in the centrifugal process, different components in the sample have more opportunities to participate in the layering process, the situation that part of stem cells are missed due to insufficient participation is avoided, and the stem cell separation accuracy is remarkably improved.

The invention discloses a rare cell capturing device and a cell heterogeneity analysis method, the device comprises: a sample liquid inlet for receiving a sample liquid; the first conveying channel is connected with the sample liquid inlet; the filtering area is connected with the first conveying channel and is used for filtering the sample liquid to obtain target liquid; the cell zoning and separating area is connected with the filtering area and is used for capturing rare cells from the target liquid; the second conveying channel is connected with the cell partition separation region; and the filtrate outlet is connected with the second conveying channel and is used for outputting the filtrate. The cell separation is realized, and the cell purity, the recovery rate and the sample capacity are improved. The method can be widely applied to the technical field of cell separation.

The blood cell separator comprises an outer shell and two second hoses, a sample inlet and an article outlet of the outer shell are fixedly communicated with first hoses, the two first hoses and the second hoses are connected through butt joint structures, the butt joint structures comprise butt joint assemblies, butt joint assemblies and butt joint assemblies, and the butt joint assemblies are connected with the butt joint assemblies. The butt joint assembly comprises a first connector and a second connector, the first connector is in a T shape, a round hole is formed in the second connector, and the first connector is inserted into the round hole of the second connector. According to the blood cell separator with the good sealing performance, when blood cells are separated and connected with other components, operation is conducted through the butt joint structure, only the first connector needs to be inserted into the second connector and rotated during butt joint, operation is convenient and fast, stability and reliability are achieved after butt joint, the sealing performance of the butt joints is guaranteed through cooperation of the sealing rings, and the sealing performance of the butt joints is improved. And the practicability of the device in use is improved.

Devices and methods suitable for cell separation. The devices herein include non-random voids interconnected through non-random pores and / or non-random solid geometrical structures optionally connected through solid non-random interconnecting elements. Such devices are preferably suitable for affinity-based cell isolation techniques which rely upon binding interactions.

This invention discloses a cell cluster separation device and its usage method in malignant pleural effusion, belonging to the field of cell separation technology. By having two locking blocks approach each other and disengage from the locking groove, the locking state between the support base and the mounting base is released, allowing the support base and multiple centrifuge tubes to be removed from the centrifuge. When removing the centrifuge tubes, because the central axis of the tube rack and the support base is parallel, the centrifuge tubes can be directly pulled vertically upwards, facilitating the simultaneous removal of multiple centrifuge tubes. The downward movement of the conical seat can compress the two locking blocks, causing them to engage in the locking groove, thus achieving the purpose of locking the support base and the mounting base. Therefore, the modular design of the centrifuge, support base, and tube rack enables the rapid loading and unloading of batches of centrifuge tubes. The use of two sets of support bases and multiple tube racks for centrifugation allows for alternating loading and unloading of centrifuge tubes, shortening the time spent loading and unloading centrifuge tubes and facilitating the efficient and stable separation of cell clusters.

The application provides an umbilical cord mesenchymal stem cell separation device, and belongs to the technical field of cell separation, comprising a support table, a support frame is fixedly connected to the support table, a top of the support frame is fixedly connected with an umbilical cord segmentation table for conveniently preprocessing the umbilical cord, a processing assembly is fixedly connected to the support table, a separation assembly for separating stem cells from umbilical cord Wharton's jelly is arranged in the processing assembly, and a cutting assembly for cutting the umbilical cord Wharton's jelly is fixedly connected to the processing assembly. The application is excellent in automation and continuity of operation process, and after the Wharton's jelly separated by the segmentation table is placed on the cutting table, a series of operations such as subsequent cutting, enzymolysis, shock mixing and centrifugation can be automatically completed under the cooperative operation of each component of the device without frequent manual intervention, so that the working efficiency is greatly improved, and the continuity and stability of the separation process are ensured.

The application discloses a PBMC separation and displacement method, and relates to the technical field of cell separation. The method comprises a separation stage and a displacement stage, and both the separation stage and the displacement stage adopt a centrifugal cup with one-in-two-out, which is provided with an inner port, an outer port and a central port. The separation stage comprises the following steps: pumping gradient liquid into the centrifugal cup from the outer port, then pumping blood samples into the centrifugal cup from the outer port, rotating the centrifugal cup and discharging inner-layer blood plasma from the central port, performing centrifugal separation while continuously pumping gradient liquid into the centrifugal cup from the outer port, discharging red blood cells from the inner port after centrifugal separation, then discharging red blood cells from the outer port, collecting product liquid and gradient liquid and discharging them to a product bag from the inner port to obtain a displacement liquid, and discharging waste liquid from the outer port. The displacement liquid is pumped into the centrifugal cup from the inner port, and the displacement stage is started. After the displacement is completed, washing waste liquid is discharged, and a PBMC product liquid is obtained.

The invention provides a separation method and an identification method of bactrian camel testis sertoli cells, and belongs to the technical field of cell separation. The method comprises the following steps: mixing 0.08-0.12% of collagenase IV solution and 0.23-0.27% of trypsin-EDTA (Ethylene Diamine Tetraacetic Acid) according to a volume ratio of 1: 1, digesting a bactrian camel testis tissue block, and then combining differential adhesion and hypotonic treatment to obtain bactrian camel testis supporting cells with the purity of 95% or above, meanwhile, reducing cell damage, and enabling the cell survival rate to exceed 85%; and the identification is completed by combining morphological observation, immunofluorescence staining and secretion function detection. The separated bactrian camel testicular sertoli cells can be used for double-hump reproductive performance research, and a foundation is laid for the reproductive performance of bactrian camels.

The invention relates to the technical field of tumor detection and cell separation, in particular to a functionalized magnetic bead suspension, a preparation method thereof, a kit and application of the kit. Comprising the following steps: S1, obtaining magnetic microspheres, performing surface activation treatment to obtain surface-activated magnetic beads, and averagely dividing the magnetic beads into two parts; s2, coupling one part of the magnetic beads with a non-cleavable chain to obtain first functional magnetic beads; s3, coupling the other part of magnetic beads with a cleavable chain to obtain second functional magnetic beads; and S4, respectively adding the first functional magnetic beads and the second functional magnetic beads into a buffer solution containing bovine serum albumin for sealing treatment, and then mixing the first functional magnetic beads and the second functional magnetic beads after sealing treatment to obtain the functionalized magnetic bead suspension. The dual functions of irreversibly anchoring white blood cells and releasing and capturing tumor cells can be achieved, the signal-to-noise ratio is increased through physical shielding, and the activity of the tumor cells is protected through mild release.

This invention provides a method for isolating and identifying interstitial cells from Bactrian camels, belonging to the field of cell isolation technology. A mixture of 0.08–0.12% collagenase IV solution and 0.23–0.27% trypsin-EDTA at a volume ratio of 1:2 is used to digest Bactrian camel testicular tissue blocks. The mixture is then purified by hypotonic treatment to remove excess germ cells, differential adhesion purification to remove a large number of supporting cells, and Percoll density gradient centrifugation to remove a small number of supporting cells. The suspension, obtained from a 34%–60% Percoll gradient, is transferred to a new culture flask to obtain interstitial cells with a purity ≥95%. Identification is performed using morphological observation, immunofluorescence staining, and secretory function testing. The interstitial cells isolated from Bactrian camels using this invention can be used for research on the reproductive performance of Bactrian camels, laying the foundation for understanding the reproductive performance of Bactrian camels.

The invention relates to the technical field of cell separation, in particular to a method for separating cartilage cells from cartilage cell spheres and application of the method. The method for separating the cartilage cells from the cartilage cell spheres comprises the step of carrying out digestion treatment on the cartilage cell spheres by adopting digestive juice, the digestive juice comprises type I collagenase, type II collagenase and streptavidin. The method can rapidly and efficiently digest the cartilage cell balls and significantly improve the survival rate of the obtained single cells, the separated single cells can maintain the phenotypes of original cartilage cells, cannot cause changes of cartilage cell attributes, can completely reserve the molecular characteristics of the cartilage cells, and can be used for comprehensively evaluating the quality attributes of the cartilage cell balls, and the method has good application prospects. And technical support is provided for promoting industrialization and commercialization processes of cartilage cell sphere related products.

The invention discloses a single cell suction device and method, and relates to the technical field of single cell separation.The single cell suction device comprises a multi-axis adjusting platform, a suction mechanism, a sample moving platform, a sample holder, a focusing module and an imaging system.The output end of the multi-axis adjusting platform is connected with the suction mechanism, and the output end of the sample moving platform is connected with the sample holder; the suction mechanism is located above the sample holder, the sample holder is used for placing a single cell sample, the focusing module is located below the sample moving platform and can focus the sample holder and the tail end of the suction mechanism, and the imaging system is located below the sample moving platform and can focus the sample holder and the tail end of the suction mechanism. The multi-axis adjusting platform is used for moving the tail end of the suction mechanism to the center of the imaging system, the sample moving platform is used for driving the sample rack to move and enabling the single-cell sample on the sample rack to be located at the center of the imaging system, and the suction mechanism is used for sucking the single-cell sample on the sample rack. According to the invention, automatic needle alignment and automatic stable suction and release of target cells can be realized, and the operation difficulty is reduced.