45 results about "Conglutination reaction" patented technology

An immunoassay comprises the steps of: (a) mixing a whole blood sample with sensitized insoluble carrier particles smaller than erythrocytes to cause an immune agglutination reaction; (b) introducing the resulting immune agglutination reaction mixture including agglutinated particles and unagglutinated particles to a flow cell, irradiating the particles passing through the flow cell with laser light, and detecting scattered lights generated thereby; (c) setting a threshold value for distinguishing unagglutinated particles from agglutinated particles and a threshold value for distinguishing the agglutinated particles from blood cells with regard to intensity of the scattered light; and (d) distinguishing and counting the unagglutinated particles, the agglutinated particles and the blood cells from the scattered lights detected in the step (b), in reference to the threshold values set in the step (c).

The invention relates to an enterovirus 71 type latex agglutination detection kit, preparation and application. The kit comprises the following substances: (1) a carboxylated latex reagent sensitizing a prokaryotic expression enterovirus 71 type VP1 specific antigen; (2) a carboxylated latex reagent sensitizing three strains of monoclonal mixed antibodies aiming at enterovirus 71 type VP1; (3) enterovirus 71 type positive-negative standard serum, an inactivated virus solution and PBS; and (4) a toothpick or a plastic cement rod which contains a glass slide platform for using the sentization latex to perform aggregation reaction and is used to uniformly mix latex and to-be reacted serum. The kit can detect EV71 antigen in samples of different sources, and overcomes the disadvantages that the sensitized antigen is less in amount and unstable, sensitized protein is easy to fall off, and the like when protein sensitizes common latex. The detection method is applicable to enterovirus 71 type serum epidemiological investigation, clinic assistant diagnosis, regulation and control on EV71 virus replication, anti-virus therapy medicine screening and other scientific research fields.

The present invention relates to an inert carrier Escherichia coli and its potential application, the Escherichia coli isolated strain has the inert carrier property, and is expected to be developed into a carrier strain for an indirect agglutination test. The inert carrier Escherichia coli is preserved in the China General Microorganisms Collection and Management Center (CGMCC), and a preservation address is Beijing, China, a preservation number is CGMCC No. 17339, a preservation date is March 18, 2019, a classification name is Escherichia coli, and a strain code is SE1. The Escherichia coli isolated strain is from a healthy flock, the bacteria number at a higher working concentration does not cause any macroscopic agglutination reaction with various chicken serums of different genetic backgrounds, and does not generate non-specific agglutination reaction with chicken source serum, so that the Escherichia coli is called as an inert carrier Escherichia coli. The application provides an inert carrier strain and its potential application for developing a simple and rapid indirect agglutination assay.

The invention discloses an inert carrier indirect agglutination test detection system and application thereof. The system comprises inert carrier bacteria S9 and a complex S9-P which is shown and expressed on the surface of the inert carrier bacteria S9 and carries a P-antibody factor. The system only carries the P-antibody factor, is single in component and specific in targeting, generates macroscopic positive particle agglutination reaction with whole blood or serum of chicken infected by pullorum disease and salmonella typhimurium under a certain concentration condition, and does not generate non-specific cross agglutination reaction with chicken-derived serum and whole blood of different backgrounds infected by non-pullorum disease and salmonella typhimurium. The S9-P is based on a glass plate agglutination reaction operation platform. The S9-P is simple and convenient to operate, sensitive and rapid in reaction, macroscopic in agglutination reaction particles and clear and easy inresult judgment, tests and result judgment are completed within two minutes, and the S9-P is suitable for targeted specific detection of pullorum disease and salmonella gallinarum infection in chicken flocks and has a good application prospect in on-site monitoring and diagnosis of the chicken flocks.

The invention provides a hybridoma cell strain capable of secreting anti-human AB blood type IgM type monoclonal antibodies. The preservation number of the hybridoma cell strain is CGMCC NO. 18535. The invention also provides the monoclonal antibody secreted by the hybridoma cell strain capable of secreting anti-human AB blood type IgM type monoclonal antibodies, and application of the monoclonalantibody. A preparation method comprises following steps: taking a human AB type erythrocyte suspension as an antigen to immunize BALB/c mice; carrying out immunization, cell fusion, screening and cloning to obtain a hybridoma cell strain 2D12 capable of stably subculturing and secreting anti-human AB blood type IgM type monoclonal antibodies; the secreted 2D12 antibody can agglutinate with humanA-type red blood cells, B-type red blood cells and AB-type red blood cells, no agglutination reaction with human O-type red blood cells is caused, the secreted 2D12 antibody is an anti-AB-type blood type monoclonal antibody, and has antibody titer of 512.

The invention relates to the field of medicine and health and provides a method for simple, rapid and effective separation of erythrocytes. According to the method, immunological agglutination technology and magnetic separation technology are combined, an antigen-antibody compound is formed by an ovine antibody against human erythrocytes and an erythrocyte, then the antigen-antibody compound and a magnetic microsphere coated by a mouse monoclonal antibody against ovine Fc-terminal undergo immunoadsorption so as to induce a more intense agglutination reaction, and magnetic separation is carried out so as to achieve the purpose of removal of erythrocytes. The method realizes simple and rapid removal of erythrocytes in samples and can be applied on a fully-automatic or semi-automatic chemiluminescence apparatus so as to realize whole blood detection of a common kit.

The invention relates to a fast check reagent of Salmonella causing food poisoning, in particular to a fast check reagent of food source type Salmonella aertrycke, Salmonella suipestifer, Salmonella enteritidis, TAB vaccine and salmonella B and a preparation method thereof. The fast check reagent has the characteristics of high speed, high accuracy, high sensitivity, and the like. Compared with a traditional method, the fast check reagent has an application value and has an important meaning for guiding clinical diagnosis and treatment and avoiding abusing antibacterial medicines. Specific Salmonella polyvalent and monovalent serum are added in enriched liquid, the check diagnosis process is shortened to 2-6 hours, the serum agglutination reaction of specific serum can be utilized at the time of enriching, and the identification accuracy is larger than 97 percent. The fast check reagent has little time consumption, high sensitivity and strong accuracy when checking pathogenic bacteria.

A reconstituted polypeptide characterized by inducing apoptosis in nuclear blood cells having integrin associated protein (IAP) without causing the agglutination of erythrocytes. This reconstituted polypeptide contains at least two H chain V domains and at least two L chain V domains of a monoclonal antibody which induces apoptosis in nuclear blood cells having IAP. This reconstituted polypeptide is useful as a remedy for blood diseases such as leukemia.

The invention discloses generic inert carrier salmonella S9H and a potential application thereof. The generic inert carrier salmonella S9H is obtained by continuously culturing inert carrier bacteriaS9 in vitro by using an LB solid and liquid culture medium for passage to the fortieth generation, under the condition of the quantity of bacteria with working concentration, the non-specific agglutination reaction with serum and whole blood of human sources, mouse sources, cattle sources, pig sources and poultry sources (including chickens, ducks, gooses, turkeys, pigeons and quails) can be avoided, the characteristics of carrying and surface expression displaying human sources, mouse sources, cattle sources, pig sources and poultry sources (including chickens, ducks, gooses, turkeys, pigeonsand quails) for different resistance factors are realized, the generic inert carrier salmonella S9H can be applied to development of an indirect agglutination test detection method for simply, conveniently and quickly detecting human and various animal antigens or infected antibodies, improves and perfects the technical bottleneck of poor specificity and sensitivity of an existing agglutination test for agglutination antigen and antibody detection, and has a wide application value and market prospect.

The present invention relates to a method for selectively detecting a multimer type multimer-forming polypeptide in a biological sample, the method comprising: (a) bringing the biological sample into contact with an agglutination reaction inducing agent to induce the formation of an aggregate in an analysis target, the agglutination reaction inducing agent being a particle in which a specific antibody is surface-bonded with the multimer-forming polypeptide; (b) obtaining an image with respect to the aggregate of step (a); and (c) analyzing a size or a shape of the aggregate by using the image. Step (a), step (b), or steps (a) and (b) are performed on a microchip having a microchannel. The image analysis is performed using a coefficient according to the size of the aggregate in a predetermined volume provided by the microchannel. In the case where the multimer type of multimer-forming polypeptide is present in the biological sample, the size of the aggregate is larger than the size of an aggregate of a monomer type control group. According to the present invention, unlike in a detection method using chemiluminescence immunoanalysis of the related art, an image with respect to an agglutination reaction target is obtained and then a size or a shape of an aggregate is analyzed so as to determine whether or not an analysis target is present in a biological sample and to determine the quantity of the analysis target. Also, it is possible to detect a multimer type multimer-forming polypeptide by just analyzing an image acquired from the sample so that the detection process is made more convenient and quick.

The invention provides a detection method for an agglutination test of rabies virus neutralizing antibody cells. In the detection method, cells for expressing rabies virus glycoprotein are taken as antigens and carrier particles; and through agglutination reaction with human, dog and cat serum antibodies, whether the rabies virus neutralizing antibodies in the serum reach an immune protection level can be judged. By the detection method, the problems that the conventional detection method for the rabies virus neutralizing antibodies is long in detection time, complicated in operation, high in detection cost and the like can be solved.

A method and a sample analysis apparatus having a processing part for practicing the method, the method including a step of, with respect to an immunoagglutination reaction that occurred in the reaction solution between the test substance and a binding partner thereof, determining an index value relating to a difference between a changing speed of a scattered light intensity in a first period of the reaction and a changing speed of a scattered light intensity in a second period of the reaction, based on a scattered light intensity of the reaction solution in the first period and a scattered light intensity of the reaction solution in the second period, and a step of determining whether the concentration of the test substance is within a proper range by comparing the index value and a predetermined threshold value.

The invention discloses generic inert carrier salmonella S9H and a potential application thereof. The generic inert carrier salmonella S9H is obtained by continuously culturing inert carrier bacteriaS9 in vitro by using an LB solid and liquid culture medium for passage to the fortieth generation, under the condition of the quantity of bacteria with working concentration, the non-specific agglutination reaction with serum and whole blood of human sources, mouse sources, cattle sources, pig sources and poultry sources (including chickens, ducks, gooses, turkeys, pigeons and quails) can be avoided; due to the characteristics of carrying and surface expression displaying human sources, mouse sources, cattle sources, pig sources and poultry sources (including chickens, ducks, gooses, turkeys,pigeons and quails) for different resistance factors are realized, the generic inert carrier salmonella S9H can be applied to development of an indirect agglutination test detection method for simply,conveniently and quickly detecting human and various animal antigens or infected antibodies, improves and perfects the technical bottleneck of poor specificity and sensitivity of an existing agglutination test for agglutination antigen and antibody detection. The product has wide application value and market prospect.

The invention provides a preparation method of pork blood tofu, and belongs to the technical field of food processing. In order to ensure the safety of blood sources, animal quarantine is performed onmixed pork blood, and continuous processing can be performed after the inspection is passed. After a blood vessel ruptures, a platelet aggregation reaction occurs, and therefore, blood is coagulated.In order to prevent the agglutination reaction, sodium citrate is used in an early stage to prevent blood coagulation, and then a coagulant is added in a later stage to completely coagulate a product, so that a stable product taste is ensured. High-pressure all water sterilization is performed after collected filtered liquid and coagulant are mixed so that the pork blood tofu can be stored at a room temperature, and product safety is guaranteed.