7 results about "Cryopreserved Tissue" patented technology

This utility model discloses a multifunctional support device for fixing and extracting cryopreserved tissues in a liquid nitrogen tank, relating to the field of biological specimen preservation technology. It mainly consists of a liquid nitrogen tank, limiting guide rails, an outer frame, a tray, and a lifting rod. The liquid nitrogen tank has an insulating layer to reduce liquid nitrogen evaporation; the limiting guide rails are arranged in a rectangular vertical "L" shape to ensure the stability of the outer frame's sliding; the outer frame has partitions dividing the storage chamber, and a locking plate combined with a spring can fix the tray; the lifting rod and sleeve engage through a J-shaped slot and locking protrusion for easy extraction of the outer frame. Furthermore, the outer frame and the outside of the liquid nitrogen tank are respectively equipped with a first and a second electronic tag for convenient sample information management. This device is easy to operate, effectively protects samples, and improves the practicality and safety of liquid nitrogen tanks in biological specimen preservation.

A method for RF rewarming of cryopreserved tissues, organs, cartilage, arteries, and the like, whereby the samples are uniformly, volumetrically rewarmed to promote viability. The method maintains a warming rate above the critical warming rate of a CPA, preferably DP6 or VS55, during the critical warming period (−80° C. to −30° C.) and does not produce cracks, crystallization, or destructive thermal gradients during devitrification. The method also minimizes CPA toxicity. The method controls frequency and impedance matching to keep the system at resonant frequency and an input impedance of 50 Ω.

The invention discloses a method for directly cryopreserving pig ear edge tissues by cycloastragenol and application of cycloastragenol, and relates to the technical field of refrigeration or cryopreservation. On the basis of an existing refrigerating fluid formula, the concentration of DMSO is reduced, high-glucose DMEM is used for replacing DPBS, meanwhile, trehalose, cycloastragenol and the like are added, and a brand-new refrigerating fluid formula is provided. The method has the following advantages: 1) an unfreezing solution and an equilibrium solution do not need to be additionally prepared; the unfreezing method is easy to operate, only simple melting is needed, and the method is suitable for large-scale preservation. And 3) the tissue does not need to be subjected to complex pretreatment, and only the tissue needs to be simply cleaned. The method has the advantages that the method is suitable for field sampling, strict sterile conditions are not needed, the cell survival rate, the cell apoptosis rate, the mitochondrial membrane potential, the active oxygen, the cell proliferation rate and the like are not obviously different from those of cells cultured from fresh tissue, and the volume of the supported cryopreserved tissue is the maximum cryopreserved volume in the cryopreserved pig tissue which can be found at present.

The application provides a glass-like cryopreservation solution and application thereof, and belongs to the technical field of tissue cryopreservation. The glass-like cryopreservation solution provided by the application contains specific final concentrations of nitrogen ketone, cellobiose and alpha-lactose, and overcomes the problem of reduced activity of stem cells in the process of umbilical cord Wharton's jelly cryopreservation in the prior art. The glass-like cryopreservation solution provided by the application can make the cryopreserved tissue well preserved at low temperature, so that the tissue can produce a low degree of dehydration in a short time but does not affect physiological properties, and is more conducive to preservation. When the tissue contains stem cells, the glass-like cryopreservation solution provided by the application can effectively avoid the problem that the stem cells in the tissue are caused to die in large quantities due to ice crystal formation in the cryopreservation process, and when the tissue is resuscitated, the stem cells can be quickly extracted, without affecting the activity of the stem cells, and without significant difference compared with fresh tissue.

The invention relates to the technical field of biology, in particular to a cryopreservation solution and a method for cryopreservation and resuscitation of umbilical cord tissue blocks, preparation of mesenchymal stem cells and establishment of a seed bank.The cryopreservation solution is prepared from 85% of a serum-free medium, 5% of platelet lysate and 10% of DMSO. After being cooled according to a program, the tissue blocks are stored in a liquid nitrogen deep hypothermia environment, the mesenchymal stem cells are cultured after the tissue blocks are recovered, the growth state of the cells can be obviously improved through serum-free culture, and differentiation osteogenesis experiments and differentiation adipogenesis experiments are carried out on the cells cultured by the cryopreserved tissue blocks, so that the differentiation function is very good. The method provided by the invention can effectively reduce waste of stem cell resources and damage to cells. According to the method, umbilical cord tissue blocks can be stored for a long time, nutrient substances required by cell growth are effectively provided, the condition of poor cell growth state is improved, and the umbilical cord tissue blocks can be recovered and amplified according to needs and then applied to treatment of future diseases.

Aspects of the disclosure include cryopreserved tissue fibers and multilayer structures comprising said fibers, wherein the fibers encapsulate dissociated cells, and where the fibers and / or structured are cryopreserved prior to aggregation of the encapsulated cell population. Methods of making and using such fibers and structures are also provided.

Bladder cancer primarily affects older adults, with radical surgery often leading to significant complications due to the use of bowel tissue for bladder reconstruction. Provided herein is a new approach using cryopreserved tissue from cadaveric vessels that avoids these complications. This new method makes surgery safer and recovery quicker, reducing hospital stays and improving patients' quality-of-life. This disclosed method is a major advancement in treating muscle-invasive bladder cancer, offering minimal side effects and a faster return to normal life.