30 results about "Cryopreserved Tissue" patented technology

A bioreactor and methods of using same for making tissue constructs and for conditioning tissue-engineered constructs and harvested tissues such as cryopreserved tissues. The bioreactor allows for static and dynamic culture / conditioning. The bioreactor is dual chambered (one chamber above and one below the cells or construct) to allow for application of biochemical and / or biomechanical stimuli to each side of the cells / construct.

A bioreactor and methods of using same for making tissue constructs and for conditioning tissue-engineered constructs and harvested tissues such as cryopreserved tissues. The bioreactor allows for static and dynamic culture / conditioning. The bioreactor is dual chambered (one chamber above and one below the cells or construct) to allow for application of biochemical and / or biomechanical stimuli to each side of the cells / construct.

The invention discloses a cryopreservation method of umbilical cord tissue blocks, and relates to a cryopreservation method of umbilical cords. The invention aims at solving the problems that the preservation time of umbilical cord tissues is short, and the cell death of the umbilical cord tissues is easily caused at present. The method comprises the following steps: 1, carrying out treatment of the tissue blocks, namely in a sterile operating platform, carrying out disinfection treatment on an umbilical cord, cleaning the umbilical cord normal saline, shearing the umbilical cord into segments, peeling off a wharton jelly, placing the wharton jelly in a clean centrifuging tube and cutting the wharton jelly into pieces; and 2, adding DMEM in the tissue blocks, resuspending the tissue blocks, then adding a pre-cooling cryopreservation liquid, sub-packaging the tissue blocks in cryopreservation tubes, cooling by use of a program-controlled cooler, and transferring the tissue blocks in liquid nitrogen for long-time storage after bacterial detection, microbiological detection and virus detection are all negative, so as to finish the cryopreservation of the umbilical cord tissue blocks. The directly-inoculated adherent survival rate of the cryopreserved and unfrozen tissue blocks in culture bottles is more than 98%, the cell growth is good, and moreover, an osteogenic differentiation experiment carried out on the cultured cells of the cryopreserved tissue blocks indicates that the differentiation function is high. The method is used for storing the umbilical cord tissues.

The present invention relates to a method and composition for the cryopreservation of adipose tissue with the intention to use this tissue in the culturing of stem and / or progenitor cells. The method uses a specific cryoprotection medium to prevent damage of the original tissue during the cryopreservation while still maintaining a high viability of the stem and / or progenitor cells obtained from the cryopreserved adipose tissue. Furthermore the cryoprotection medium of the present invention does not contain any kind of xenogeneic sera, a critical factor since it is the intention of that the cryopreserved tissue is used for obtaining stem and / or progenitor cells that can be used in medicine. The cryoprotection medium is characterized in that it is a solution of physiological water comprising glycerol and sucrose and / or trehalose and optionally serum albumin.

An method for enhanced follicular tissue transplantation and management is provided. Follicular tissue is first obtained from a donor during a dedicated harvesting procedure or as a by-product of another cosmetic surgery procedure, then cryopreserved and stored. When implantation is desired, the cryopreserved tissue is thawed, and the tissue that survived the cryopreservation and thawing processes is implanted in the recipient. In another aspect of the invention, the harvesting process is conducted when the quality of donor follicular tissue is as optimal as possible, and is stored in cryopreserved form until the donor requires implantation thereof, such that the newly implanted thawed follicular tissue is of higher quality than the recipient's current follicular tissue. In yet another aspect of the present invention, a method for managing the business aspects of the transplantation process, including harvested follicular tissue grading, cataloguing and banking is provided.

The invention discloses a preparation and cryopreservation method of human placental chorionic tissues. The method comprises the following steps: (1) separation and cryopreservation of a placental amnion and a decidua; (2) separation and cryopreservation of placenta subchorionic large vascular tissues; 3) separation and cryopreservation of the placental chorionic tissues; and (4) treatment and cryopreservation of the placental chorionic tissues. The invention also provides a method for resuscitation after cryopreservation. The cryopreservation method facilitates the improvement of the activityof cryopreserved tissues and cells, the morphology, the function and the structure of resuscitated cryopreserved tissues are same to those of fresh tissues, the overall survival rate of cells in thetissues reaches 90% or above, and the preserved tissues can be used in the field of separation of stem cells and epithelial cells, and also can be used in the field of tissue engineering transplantation.

The application provides a tissue and / or cell cryopreservation protective solution as well as preparation and application thereof. The tissue and / or cell cryopreservation protective solution comprisesa permeating protective agent and a non-permeating protective agent, wherein the cryopreservation protective solution further comprises platelet lysates. In addition, the application further providesa method for cryopreserving tissues and / or cells by using the tissue and / or cell cryopreservation protective solution provided by the application, as well as a cryopreserved tissue and / or cell and use of the cryopreserved tissue and / or cell.

The invention belongs to the technical field of biology, and discloses a method for preparing mesenchymal stem cells. The method takes a neonatal umbilical cord as a raw material and comprises the following steps: pretreating the umbilical cord; cryopreserving tissues; resuscitating the tissues; separating primary mesenchymal stem cells; and amplifying the mesenchymal stem cells. The method disclosed by the invention has the advantages being easy for industrialization and operation and the like, and can be used for cryopreserving a large quantity of umbilical cord tissues and resuscitating the umbilical cord tissues for clinical need to prepare the mesenchymal stem cells. Compared with an existing method for directly separating the mesenchymal stem cells and cryopreserving the mesenchymal stem cells, the method disclosed by the invention can be used for sufficiently preserving the most original mesenchymal stem cells contained in the umbilical cord tissues, thereby greatly reducing the preservation cost and preventing the mesenchymal stem cells from influencing clinical application after being differentiated due to multiple-time passage in vitro.

The invention discloses a method for preparing and freezing human placental chorionic tissue, comprising: (1) separating and freezing the placental amniotic membrane and decidua; (2) separating and freezing the large blood vessel tissue under the placental chorionic tissue; 3) Separation and cryopreservation of placental chorionic tissue; (4) Processing and cryopreservation of placental chorionic tissue. The invention also provides a recovery method after cryopreservation. The cryopreservation method of the present invention is beneficial to improve the activity of cryopreserved tissues and cells, and the shape, function and structure after resuscitation are consistent with those of fresh tissues, and the overall survival rate of cells in the tissues is over 90%, and the preserved tissues can not only be used to separate stem cells , epithelial cells and other fields, and can also be used in fields such as tissue engineering and transplantation.

A solution system for promoting recovery of cryopreserved tissues and organs and cell activity of the present invention uses one or more of water and polyols as the base solution, and polyphenols, citric acid, and poloxamer are added to the base solution and vitamin E, put tissues, organs and cells in this solution system for cryopreservation, and apply the wavelength within the range of 20KHz-1MHz and the sound intensity is not greater than 3W / cm after cryopreservation 2 Cells are irradiated by ultrasonic waves. By adjusting the ratio of different components in the solution, and controlling the wavelength, sound intensity and irradiation time of ultrasonic waves, the biological activity of frozen tissues, organs and cells after recovery can be improved.

The invention discloses a method for preparing and freezing human placental subchorionic macrovascular tissue, comprising: (1) washing the placenta; cutting off the decidua along the edge of the placenta to remove the amniotic membrane; (2) removing the amniotic membrane from the placenta The fetal facial chorion and large blood vessels are separated together, washed with normal saline or PBS buffer, and the blood vessels are flushed to remove blood clots in the blood vessels; then, the large blood vessels connected to the chorionic plate are cut off one by one; (3) the blood vessels are transferred Put it into a cryopreservation tube or a cryopreservation bag, introduce the vitrification solution in a three-step method, transfer it to a programmed cooling device, cool it down to -80°C to -90°C, and transfer it to liquid nitrogen for cryopreservation. The cryopreservation method of the present invention is beneficial to improve the activity of cryopreserved tissues and cells, and the morphology, function, and structure after recovery are consistent with those of fresh tissues. The preserved tissues can not only be used in the fields of separating stem cells and epithelial cells, but also in tissue Engineering transplantation and other fields.

The invention provides a deep cryogenic freezing method for tumor tissue blocks, and relates to tumor tissue freezing methods. The deep cryogenic freezing method is used for solving the problems of the existing tissue cryopreservation methods that the activity of resuscitated tumor cells is remarkably lowered and the tumor forming rate is low. The method comprises the following steps: (1) preparing a cryopreservation solution; (2) treating and freezing the tissue blocks; (3) resuscitating cryopreserved tumor tissue blocks. According to the method, tumor tissue cryopreservation is carried out by using a permeable and impermeable cryopreservation protectant combined method, meanwhile, a preparing proportion of reagents is optimized, the proportion of DMSO in the cryopreservation solution is lowered to 2%, the cytotoxicity of tissue is lowered, a very good protective action on the cryopreserved tissue is achieved, thus, the cryopreserved tissue has relatively high mouse tumor forming performance after resuscitation, and the tumor forming rate reaches 90% or more. The method is applied to the freezing of the tumor tissue.

The invention discloses a method for preparing and freezing human placental amniotic membrane and decidua tissue, comprising: (1) washing the placenta; cutting off the decidual membrane along the edge of the placenta, and dividing the amniotic membrane into tissue pieces; The amniotic membrane on the fetal surface of the placenta is removed from the attached placental tissue; (2) Wash the amniotic membrane and decidua with normal saline or PBS buffer solution, fold or lay the amniotic membrane and decidua into a cryopreservation bag or cryopreservation tube , add 4°C cryopreservation solution, seal, transfer to a programmed cooling device, cool down to -80°C according to the set cooling program, and transfer to liquid nitrogen for cryopreservation. The cryopreservation method of the present invention is beneficial to improve the activity of cryopreserved tissues and cells, and the shape, function and structure after recovery are consistent with fresh tissues, and the overall survival rate of cells in the tissues is over 90%, and the preserved tissues can not only be used for separation Stem cells, epithelial cells and other fields can also be used in fields such as tissue engineering and transplantation.

The invention discloses a method for preparing and freezing human placental villi, comprising: (1) washing the placenta; cutting off the decidua along the edge of the placenta to remove the amnion; (2) removing the placenta from which the amnion has been removed The fetal facial chorion and large blood vessels are separated together, and the remaining placental tissue is the placental villi tissue. Cut the placental villi tissue into small pieces, and then cut them into thin slices; put the thin slices into cryopreservation bags or tubes, and proceed in three steps. Introduce the vitrification solution by using the method, transfer to the program cooling device, cool down to -80°C to -90°C, and transfer to liquid nitrogen for cryopreservation. The cryopreservation method of the present invention is beneficial to improve the activity of cryopreserved tissues and cells, and the shape, function, and structure after recovery are consistent with those of fresh tissues, and the overall survival rate of cells in the tissues is over 90%, and the preserved tissues can not only be used to separate stem cells , epithelial cells and other fields, and can also be used in fields such as tissue engineering and transplantation.

The invention discloses a systematic method for preparing and freezing placental tissue according to structural levels, including: (1) separation and freezing of placental amniotic membrane and decidua; (2) separation and freezing of placental subchorionic large blood vessel tissue ; (3) Isolation and cryopreservation of placental chorionic tissue; (4) Processing and cryopreservation of placental chorionic tissue. The invention also provides a recovery method after cryopreservation. The present invention separates and preserves tissues such as placental amniotic membrane, decidua, subchorionic blood vessels, and chorion, which is beneficial to improving the activity of frozen tissues and cells. The overall survival rate of inner cells is over 90%, and the preserved tissues can not only be used in fields such as separating stem cells, but also in fields such as tissue engineering and transplantation. The invention provides a systematic method for preserving various tissues of the complete placenta, and provides important biological resources for the research on stem cells derived from the placenta.

The invention discloses a cryopreservation method of umbilical cord tissue blocks, and relates to a cryopreservation method of umbilical cords. The invention aims at solving the problems that the preservation time of umbilical cord tissues is short, and the cell death of the umbilical cord tissues is easily caused at present. The method comprises the following steps: 1, carrying out treatment of the tissue blocks, namely in a sterile operating platform, carrying out disinfection treatment on an umbilical cord, cleaning the umbilical cord normal saline, shearing the umbilical cord into segments, peeling off a wharton jelly, placing the wharton jelly in a clean centrifuging tube and cutting the wharton jelly into pieces; and 2, adding DMEM in the tissue blocks, resuspending the tissue blocks, then adding a pre-cooling cryopreservation liquid, sub-packaging the tissue blocks in cryopreservation tubes, cooling by use of a program-controlled cooler, and transferring the tissue blocks in liquid nitrogen for long-time storage after bacterial detection, microbiological detection and virus detection are all negative, so as to finish the cryopreservation of the umbilical cord tissue blocks. The directly-inoculated adherent survival rate of the cryopreserved and unfrozen tissue blocks in culture bottles is more than 98%, the cell growth is good, and moreover, an osteogenic differentiation experiment carried out on the cultured cells of the cryopreserved tissue blocks indicates that the differentiation function is high. The method is used for storing the umbilical cord tissues.