53 results about "Desulfobacter sp." patented technology

The application discloses a biological electrochemical auxiliary sulfur conversion promoting sulfur-based denitrification and sulfate emission reduction method and belongs to the field of water treatment. ‑ Nitrogen-containing wastewater enters an elemental sulfur denitrification system, and NO3 0 is reduced to N2 by sulfur autotrophic denitrifying bacteria, and elemental sulfur (S 2‑ ) is converted into SO4 2‑ . Effluent enters a cathode chamber of an electrochemical auxiliary sulfate reduction system, and hydrogen autotrophic sulfate reducing bacteria utilize cathode hydrogen production to reduce SO4 ‑ to HS 2‑ , and meanwhile, alkalinity produced by cathode hydrogen production neutralizes acidity produced in the denitrification process in the elemental sulfur denitrification system. ‑ HS 2‑ / supernatant is returned to the elemental sulfur denitrification system to react with S 0 to generate polysulfide (S n 2‑ ), S n 2‑ , which is used as an efficient electron donor to participate in denitrification, thereby significantly improving denitrification efficiency. The application does not need to add an organic carbon source, sludge production is small, high-concentration nitrogen-containing wastewater is efficiently treated, and the content of sulfate in effluent is simultaneously reduced, thereby realizing sulfur resource recycling, and the application is an economic and environment-friendly autotrophic denitrification method.

The invention discloses an antibacterial peptide induced by sulfate reducing bacteria and application of the antibacterial peptide, and belongs to the technical field of marine corrosion protection. The amino acid sequence of the SRB-induced antibacterial peptide is VAHKRLVVGTR, and the SRB-induced antibacterial peptide has efficient antibacterial activity on corrosive bacteria such as SRB and has the advantages of being environmentally friendly and not prone to inducing the bacteria to generate drug resistance, and the problems that an existing antibacterial peptide is insufficient in corrosive bacteria specificity, poor in environmental stability and the like are solved; meanwhile, peptide chains are short, synthesis is easy, and the production cost is low; the compound corrosion inhibition bactericide formed by combining the compound with a chemical bactericide can significantly improve the antibacterial and antiseptic effects, saves the dosage of the chemical bactericide, effectively solves the problems of bacterial drug resistance, high cost, environmental pollution and other pain points caused by abuse of the existing bactericide, and is suitable for the fields of marine microbial corrosion protection and the like.

The present application belongs to the technical field of environmental remediation and soil ecological engineering, and particularly relates to a modified iron biochar-humus-sulfate-reducing bacteria (SRB) composite system for realizing efficient immobilization and ecological remediation of cadmium (Cd 2+ ) in heavy metal contaminated soil. The system can enhance the stability of microbial colonies and has the characteristics of physical and biological synergistic passivation, thereby realizing long-term stabilization and ecological remediation of heavy metals.

The invention relates to a method for in-situ remediation of hexavalent chromium contaminated soil and application of the method, according to the method, a nutritional additive solution and a sulfate reduction biological agent are added into the hexavalent chromium contaminated soil, and a nutritional additive contains organic acid salt. According to the method, the organic acid salt serves as a chelating agent to enable insoluble hexavalent chromium to be dissolved out of soil particles, meanwhile, the organic acid salt serves as a nutrition additive to promote reproduction and growth of sulfate reducing bacteria, sulfate is reduced into negative bivalent sulfur through the sulfate reducing bacteria, and the dissolved hexavalent chromium is reduced into trivalent chromium through the negative bivalent sulfur; finally, the purpose of treating the hexavalent chromium contaminated soil is achieved.

The invention discloses a device and a method for detecting the corrosion resistance of a cementing material by simulating a dynamic seawater impact environment, the device combines the dissolved oxygen regulation (2-8mg / L) of a corrosive medium adding system with the coupling of sulfate reducing bacteria (10-10CFU / mL) through the cooperative regulation of a flow making pump, a wave generator and a guide plate (the concentration deviation is less than or equal to + / -0.05%, and the temperature uniformity is less than or equal to + / -0.3 DEG C); chemical-biological-mechanical three-factor simulation is realized; the test piece fixing frame is of an electric rotating structure (0.5-2r / min), and is matched with dynamic impact to ensure that the test piece is uniformly impacted. According to the method, a corrosion rate curve (R is larger than or equal to 0.95) is fitted by presetting a marine environment template, closed-loop feedback control and an emergency pressure maintaining mode (the data loss rate is smaller than or equal to 0.05%). The device and the method solve the problems of single simulation and low precision in the prior art, the correlation between the detection result and the real marine environment is improved by 85%, and a reliable platform is provided for the corrosion resistance test of the ocean engineering cementing material.

The present application relates to sewage remediation technical field, particularly to a kind of in-situ microbial pumping and injection remediation method of acid uranium mining groundwater.The method provided by the present application has the following advantages:1) the present application uses contaminated raw water to culture sulfate-reducing bacteria into bacterial solution, and continuously injects bacterial solution into the area to be repaired, and supplements new culture medium, so as to realize the continuous pumping and injection of bacterial solution, by reasonably controlling the continuous pumping and injection ratio (15%, 20% pumping and injection alternately), increase the reproduction and expansion rate of functional microorganisms, enhance the self-reproduction and repair effect of in-situ microorganisms, realize the efficient removal of iron, uranium and sulfate, reduce the degree of water pollution;2) low cost: sulfate-reducing bacteria is a kind of biological catalyst that can self-reproduce, by suitable pumping and injection ratio, in-situ biological sustainable remediation effect can be realized, and the cost of groundwater pumping and treatment is reduced;3) environmentally friendly: sulfate-reducing bacteria is non-toxic and harmless to the environment, and will not cause secondary pollution.

The invention relates to the field of oil-gas field development, and discloses a method for removing sulfur-containing gas in an oil well by a biological method, which comprises the following steps: injecting a medicament A with the concentration of y1mg / L into the oil well according to a correlation formula y1 = 20a * Lgx1, x1 > 2; injecting an agent A with the concentration of y1mg / L into the oil well according to a correlation formula y1 = 20a * Lg2, wherein x1 is smaller than or equal to 2; injecting an agent B with the concentration of y2mg / L into the oil well according to a correlation formula of y2 = 30b / Lgx2, wherein x2 is greater than 2; injecting an agent B with the concentration of y2mg / L into the oil well according to a correlation formula of y2 = 30b / Lg2, wherein x2 is less than or equal to 2; wherein the concentration of the sulfate reducing bacteria in the oil well is x1CFU / mL, the concentration of the beneficial bacteria in the oil well is x2CFU / mL, a and b are actual value coefficients, a is smaller than or equal to 2, and b is larger than or equal to 1 and smaller than 10; the agent A comprises a sulfur removal bacterial agent; the medicament B comprises a nutritional agent. The reagent concentration is determined by utilizing a correlation formula, the reagent adding stability is realized, the desulfurization effect can be effectively improved, the reagent adding is more scientific, and the desulfurization cost is reduced.

The invention provides a coal gangue rapid soil formation method and application thereof in ditch filling and land reclamation, and belongs to the technical field of coal gangue ecological utilization. The coal gangue rapid soil formation method comprises the following steps that a foundation layer, a parent material-like layer, a soil-like layer and an ecological surface layer are sequentially constructed in a gully from bottom to top, and ecological stockpiling is carried out; the base layer is formed by a flexible biological impermeable material and a compound bacteria solution A; the parent material-like layer is formed by low-sulfur coal gangue, an improved matrix A and a bacillus pasteurii liquid; the soil-like layer is formed by high-sulfur coal gangue, an improved matrix B and a compound bacteria solution A; the ecological surface layer is formed by an ecological base material, a compound bacteria solution B and plant seeds; the compound bacteria liquid A in the base layer and the soil-like layer comprises acidophilic sulfate reducing bacteria and bacillus pasteurii, and the compound bacteria liquid B comprises bacillus subtilis, rhizobium and nitrogen-fixing bacteria. By adopting the method disclosed by the invention, harmless and large-scale digestion treatment of the coal gangue can be realized.

The invention belongs to the technical field of environmental materials, and particularly relates to a method for electrodeposition-assisted biosynthesis of a silver-based catalytic material SSSM-SRB and application of the silver-based catalytic material SSSM-SRB. The invention relates to a method for electrodeposition-assisted biosynthesis of a silver-based catalytic material SSSM-SRB, which comprises the following steps: by taking a sulfate reducing bacteria (SRB) suspension as an electrolyte and a silver net as a working electrode, carrying out electrodeposition by adopting a cyclic voltammetry, and growing Ag2S on the surface of the silver net in situ. The SRB is used for biologically synthesizing Ag2S, the process is environmentally friendly, and use of toxic reagents in traditional chemical synthesis is avoided.

The invention relates to the technical field of anhydrous treatment, in particular to a heavy metal adsorption filler as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, mixing biomass, attapulgite, a pore-forming agent and water, and performing compression molding to obtain raw material balls; the raw material balls are sequentially subjected to first drying and anoxic pyrolysis, and filler balls are obtained; and soaking the filler balls in a sulfate reducing bacteria solution, and sequentially carrying out biofilm formation and secondary drying to obtain the heavy metal adsorption filler. The heavy metal adsorption filler obtained by the method can effectively adsorb heavy metals, has higher mechanical strength and is suitable for repairing underground water.

The present application relates to wastewater biological treatment technical field, disclose a kind of high sulphate high nitrate nitrogen wastewater denitrification processing method, comprising the following steps: (1) in anoxic zone I, anoxic zone II and aerobic zone are filled into biological membrane carrier, and the biological membrane carrier of anoxic zone I and anoxic zone II is attached with salt-tolerant anoxic denitrifying bacteria, and the biological membrane carrier of aerobic zone is attached with salt-tolerant aerobic denitrifying bacteria;(2) the carbon-nitrogen ratio of wastewater to be treated is adjusted to 1.25~1.5, then into anoxic zone I, and carries out first-stage anoxic biological treatment;(3) the effluent of anoxic zone I is introduced into anoxic zone II, and carries out secondary anoxic biological treatment;(4) the effluent of anoxic zone II is introduced into aerobic zone, and carries out aerobic biological treatment.The method of the present application can better utilize denitrifying bacteria to inhibit the enrichment of sulfate-reducing bacteria, avoid the production of toxic sulfide and hydrogen sulfide, thereby improving the efficiency of wastewater denitrification.

The invention belongs to the technical field of oil field production, oil field chemistry and bacterium detection, and particularly relates to a ball coating bacterium detection method and device and application. The ball coating bacteria detection method provided by the invention comprises the following steps: (1) pouring a sterilized culture medium into a sterile culture dish, and adding sterile balls after the culture medium is solidified to obtain a bacteria detection device; (2) adding a certain amount of bacteria-containing water to the culture medium, and shaking the balls to uniformly coat the culture medium with the bacteria-containing water; (3) inverting the bacterium detection device, and separating the ball from the culture medium; (4) culturing the bacteria detection device to obtain a bacterial colony; and (5) identifying and counting bacterial colonies, and calculating to obtain the number and concentration of bacteria in the bacteria-containing water body. The saprophytic bacteria, the iron bacteria and the sulfate reducing bacteria can be qualitatively and quantitatively detected, the detection result is accurate and reliable, and the repeatability is high.

The application provides a sulfur-mediated anaerobic-anoxic-aerobic coking wastewater treatment method and system. The method is characterized by inoculating specific bacteria in an anaerobic reactor, an anoxic reactor and an aerobic reactor which are sequentially connected, and then making the coking wastewater to be treated sequentially pass through the anaerobic reactor, the anoxic reactor and the aerobic reactor, and then precipitating in a sedimentation tank, so as to realize wastewater treatment. In the above process, the coking wastewater to be treated sequentially passes through the anaerobic reactor inoculated with sulfate-reducing bacteria, the anoxic reactor inoculated with sulfur autotrophic denitrifying bacteria and the aerobic reactor inoculated with nitrifying bacteria, and the "sulfur cycle" is embedded in the coking wastewater treatment process, so that various pollutants in the coking wastewater can be effectively removed.

This invention relates to a sulfur autotrophic denitrification coupled with anaerobic ammonia oxidation filter and its operation method based on a backwashing strategy to achieve hydrogen sulfide reduction and sulfur source regeneration, belonging to the field of biological wastewater treatment. A backwashing device is installed at the bottom of the filter, and sulfur-containing filter media is dispersed within the filter. The filter outlet is equipped with a dissolved oxygen coupled with a temperature probe and a hydrogen sulfide probe, connected to a real-time monitoring and control system. The sulfur-containing filter media serves as a carrier for the attachment and growth of sulfur autotrophic denitrifying bacteria, sulfate-reducing bacteria, and anaerobic ammonia oxidizing bacteria. The real-time monitoring and control system is configured to activate the backwashing device when the hydrogen sulfide concentration exceeds a set value, injecting a set amount of gas into the filter for backwashing. While ensuring the smooth progress of sulfur autotrophic denitrification, anaerobic ammonia oxidation is coupled, and the oxygen from the backwashing is used to oxidize the hydrogen sulfide, converting it into elemental sulfur, which continues to serve as a sulfur source to drive sulfur autotrophic denitrification and achieve sulfur source regeneration. This effectively reduces the hydrogen sulfide content.

The invention relates to the technical field of sulfate reducing bacteria, in particular to a preparation method of a space-functionalized sulfate reducing bacteria blanket, which comprises the following steps: firstly, preparing a three-layer composite carrier consisting of a carbon felt bottom layer, a nitrogen-doped graphene / Fe3O4 conductive middle layer and a slow-release nutrition top layer; then, utilizing the synergistic effect of an electric field with specific intensity and an alternating / constant magnetic field to directionally colonize an acid-producing and hydrogen-producing flora and an acetic acid oxidation type and hydrogen nutrition type sulfate reducing flora in different space depths of the carrier in sequence to form a vertical metabolism partition; and finally, maturing the fungus blanket through multi-stage gradient domestication containing a fluctuating carbon source and weak current stimulation. According to the improvement, the problems of low treatment efficiency, poor impact resistance and long-term operation performance degradation caused by disordered spatial distribution of functional microorganisms, easy breakage of metabolic chains, unstable flora structure and lack of in-situ sensing regulation capability when the traditional sulfate reducing flora is applied to high-volatility rare earth tail water treatment are solved.

The invention discloses a multi-dimensional quantitative detection method for microbial corrosion resistance of Cr-containing steel. The method comprises the following steps: a microbial challenge test: inoculating sulfate reducing bacteria into a culture medium, and exposing a sample in a microbial environment; performing quantitative analysis on corrosion products: performing ultrasonic rust removal on the sample by using an acid pickling solution, then calculating a weightlessness corrosion rate, and quantifying the adhesion amount of microorganisms on the surface of the sample; multi-mode electrochemical testing: monitoring the open-circuit potential and linear polarization resistance of the sample, and performing electrochemical impedance spectroscopy and potentiodynamic polarization curve testing to obtain corrosion current density and polarization resistance; surface analysis: analyzing the corrosion morphology and element distribution of the sample through a scanning electron microscope and an energy disperse spectroscopy, and determining the chemical state of the chromium element by adopting X-ray photoelectron spectroscopy to obtain the stability and the sulfur content of a passive film; and evaluating the corrosion degree: establishing a coupling model of the corrosion current density, the polarization resistance, the passivation film stability, the microorganism adhesion amount and the sulfur content, and calculating the corrosion degree.

This invention discloses a microbial corrosion method for preparing Al for hydrogen production by water electrolysis. 0.6 The method for developing a CrFe2Ni2 high-entropy alloy catalyst belongs to the field of electrochemical catalysis technology. This invention uses sulfate-reducing bacteria (SRB) as the experimental strain and bulk Al as the catalyst. 0.6 A CrFe2Ni2 high-entropy alloy was used as the matrix material. After SRB corrosion, the FeS corrosion product generated on the alloy surface promoted the catalytic activity of the material. The resulting catalyst was used as the working electrode for water electrolysis to produce hydrogen, exhibiting excellent OER activity and durability in alkaline solutions. Compared with existing technologies, this invention is the first to combine microbial corrosion with bulk alloys for industrial hydrogen production via water electrolysis. The preparation method is simple and mild, with potential application prospects, and provides theoretical guidance and technical support for the design and synthesis of other non-precious metal water electrolysis catalysts.

The application of the cross-feeding composite microbial agent for simultaneously removing mineral processing reagents and heavy metal pollution belongs to environmental microbial remediation and wastewater treatment technology, and is a mixture of a sulfur-oxidizing bacteria (Thiobacillus.sp ) bacterial suspension and a sulfate-reducing bacteria Desulfotomaculum_profundi ) bacterial suspension. The cross-feeding composite microbial agent is obtained by inoculating the mixed bacterial liquid into a liquid culture medium and culturing at 30 DEG C under constant temperature and micro-oxygen conditions at 120 rpm until the OD600 value reaches 0.6-1.0. The cross-feeding composite microbial agent realizes simultaneous xanthate high-efficiency degradation and heavy metal (such as Cu²⁺, Pb²⁺ and Zn²⁺) simultaneous fixation treatment in one reactor through the metabolic synergistic effect of the sulfur-oxidizing bacteria Thiobacillus.sp ) and the sulfate-reducing bacteria Desulfotomaculum profundi ).

This invention provides a permeable reactive wall process and its application for simultaneously removing hexavalent uranium and sulfate from groundwater. The permeable reactive wall process consists of a first treatment unit and a second treatment unit arranged sequentially along the water flow direction. The first treatment unit is filled with a first reaction medium, including zero-valent iron, sulfur-modified coconut shell charcoal, and sulfate-reducing bacteria. The second treatment unit is filled with a second reaction medium, including zero-valent iron, ferric oxide, sulfur-modified coconut shell charcoal, and *Priestia* sp., a non-pathogenic and environmentally friendly bacterium that can simultaneously remove uranium from water through adsorption and reduction. The composite permeable reactive wall process provided by this invention has strong resistance to interference from other substances in the water. When used to simultaneously remove sulfate and hexavalent uranium from groundwater at 15–35°C, the removal rates of both sulfate and uranium in the groundwater are greater than or equal to 90%.

The application provides a wastewater and waste gas treatment device and method, which comprises a pool body and a gas-liquid mixed water distribution system; a flow guide area, an upflow area, a downflow area and an autotrophic bacteria treatment area are arranged in the pool body; the upflow area and the downflow area are arranged side by side between the flow guide area and the autotrophic bacteria treatment area, and each of the upflow area and the downflow area comprises a sulfate-reducing bacteria treatment section close to the flow guide area and a heterotrophic bacteria treatment section close to the autotrophic bacteria treatment area; an overflow area is arranged above the autotrophic bacteria treatment area; the gas-liquid mixed water distribution system comprises a first gas-liquid mixed passage connected with a water inlet pipe led out from the flow guide area and a water distribution pipe led out from the upflow / downflow area, and a second gas-liquid mixed passage connected with a circulating pipe led out from the overflow area and the water distribution pipe led out from the upflow / downflow area. The application can realize simultaneous nitrification and denitrification and strengthen the denitrification effect, and reduce the discharge amount of sulfate in sulfur autotrophic denitrification through autotrophic bacteria, heterotrophic bacteria and sulfate reduction, so that the efficiency is high and the energy consumption is small.

The invention discloses an acid wastewater permeable reactive barrier filling material as well as a preparation method and application thereof, and belongs to the technical field of acid mine wastewater treatment and water environment remediation, the filling material comprises the following components in percentage by mass: 20-60% of modified tailing sand and 40-80% of municipal sludge biochar. According to the permeable reactive barrier filler, the gelling performance and compressive strength of the filler are enhanced through modification of the iron ore tailings, the adsorption capacity of the filler to toxic and harmful metal is increased through the municipal sludge biochar, meanwhile, attached growth of sulfate reducing bacteria is promoted through biochar pores and organic matter contained in the biochar, and the service cycle of the permeable reactive barrier filler is prolonged; the treatment efficiency of the acid wastewater is improved. The composite filler has the characteristics of wide raw material sources, simple preparation process, rich reaction ways and the like, and has a better market application prospect in the aspects of mine wastewater treatment and water environment restoration.

The present application belongs to the field of soil remediation, and particularly relates to a method for remediation of cadmium contaminated soil. The specific technical scheme is as follows: a soil remediation method comprises the following steps: before planting economic crops, applying sulfate-reducing bacteria to the soil to be remediated; after harvesting the economic crops, planting cadmium extraction crops in the soil to be remediated. The present application provides a water-flood rotation soil remediation method which can produce while remediation. On the one hand, the biological passivation can reduce the biological availability of cadmium in the soil, thereby reducing the stress of cadmium on plants and the absorption and accumulation of cadmium by plants; on the other hand, the biological technology can strengthen the ability of nightshade to extract cadmium, thereby extracting cadmium in farmland soil.

The invention discloses a biological carbon source optimization method and application of the biological carbon source optimization method to in-situ purification of high-sulfate mine water in a goaf. Preliminarily screening cheap degradable carbon sources, and determining the material cost of each carbon source; measuring and evaluating the carbon release performance of the carbon source and the availability degree and activity of the sulfate reducing bacteria; and constructing a carbon source optimization multi-index comprehensive evaluation model according to the evaluation indexes, and selecting an optimal carbon source. And finally, preparing a structured cage body, punching right above a coal mine goaf, directly putting the cage body into the goaf through a drill hole, introducing nitrogen into high-sulfate mine water to remove oxygen, discharging the high-sulfate mine water into the goaf, and discharging low-sulfate low-suspended-matter mine water from an outlet of the goaf. According to the method, the underground mining space of the coal mine is fully utilized, the mine water treatment cost can be greatly reduced, the sulfate removal efficiency can be improved, green low-carbon treatment of the mine water is promoted, and efficient synergistic removal of sulfate and suspended solids is achieved.

This invention discloses the preparation and application of a bio-supported graphitic nitrogen carbide catalyst for efficient water electrolysis, belonging to the field of electrochemical catalysis technology. The invention uses sulfate-reducing bacteria (SRB) as the experimental strain. A nickel source and pre-prepared g-C3N4 are directly added to a culture medium containing an iron source. Utilizing the metabolic sulfidation of SRB under extremely mild conditions (33±0.2 ℃), a one-step in-situ biomineralization process generates Ni-doped FeS nanocomposite materials on the surface of g-C3N4. This invention cleverly utilizes the culture medium components as both an iron source and a nutrient source. The entire composite loading process requires no external magnetic field or other physical field intervention and no secondary high-temperature heat treatment. The obtained catalyst powder is loaded onto nickel foam (NF) as the working electrode, exhibiting excellent OER activity and durability in alkaline solutions. This method is simple, environmentally friendly, and low-cost, providing a new pathway for the large-scale production of high-performance non-precious metal electrocatalysts.

The invention relates to the technical field of microbial remediation, in particular to a microbial complex microbial inoculant for remediation of petroleum hydrocarbon contaminated soil and a remediation method and application of the microbial complex microbial inoculant. The complex microbial inoculant is composed of sulfate reducing bacteria, methanogens, oleophilic bacteria and white-rot fungi. The ratio of the sulfate reducing bacteria to the methanogens to the oleophilic bacteria to the white-rot fungi is (2-4): (0.5-2): (3-5): (1-3) according to inoculation biomass. The remediation method comprises the following steps: inoculating bacterial liquid of sulfate reducing bacteria and methanogens to petroleum hydrocarbon contaminated soil below a certain depth, and continuously remediating for 2-5 weeks; the surface layer of the contaminated soil is inoculated with bacterial liquid of oleophilic bacteria and white-rot fungi, and continuous remediation is conducted for 2-5 weeks. According to the method, a strategy of anaerobic-aerobic two stages and fungus-bacteria transboundary synergy is utilized, refined remediation of a polluted site is realized, and finally, the complex and stubborn petroleum hydrocarbon pollutants are fully degraded by utilizing a mutual synergy effect after multiple microorganisms are compounded.

The application discloses a treatment process and a combined reactor thereof for mine acid wastewater, and relates to the technical field of water treatment; and specifically comprises the following steps: oxidizing the mine acid wastewater through an aerobic reactor; adjusting the pH to 2.8 in a first neutralization reactor by using KOH, and further adjusting the pH to 4.5 in a second neutralization reactor by using KOH; introducing H2S into the wastewater into a pretreatment reactor for treatment, and continuously introducing the treated wastewater into two anaerobic reactors for reaction. The application adopts the "aerobic-anaerobic" combined reactor containing iron-oxidizing bacteria and sulfate-reducing bacteria to treat the mine acid wastewater, which can realize effective treatment of AMD on one hand, and selectively precipitate heavy metals on the other hand, so that valuable schuilingite and metal sulfides are obtained. The method realizes effective utilization of the economic value of the wastewater and the goal of environmental governance, and has good economic value.

The present application relates to water treatment and aquaculture environmental protection technical field, and disclose a bypass sulfur cycle coupled seawater aquaculture tail water deep denitrification and phosphorus removal method and system, including main road and bypass double process: S1 solid-liquid separation obtains concentrated solid phase and main road clear liquid;S2 in bypass anaerobic reactor, concentrated solid phase organic matter is hydrolyzed and acidified, and then sulfate reducing bacteria is utilized, and reduced sulfate is generated into sulfide and releases phosphorus, and bypass fermentation liquor is obtained;S3 in side flow chemical phosphorus removal unit, reagent is added, and phosphorus precipitate is generated and recovered in a direction, and sulfur-rich supernatant is obtained;S4 in main road anoxic reactor, main road clear liquid, sulfur-rich supernatant and nitrification reflux liquid complete autotrophic denitrification;S5 in main road aerobic reactor, ammonia nitrogen is oxidized into nitrate, and part of effluent is refluxed.The present application realizes spatial decoupling of electron donor and acceptor, and double sludge age operation guarantees efficiency, and external carbon source is not needed, and denitrification and phosphorus removal and phosphorus resource recovery are realized, and the seawater aquaculture tail water quality is adapted.

The application discloses a cadmium-polluted paddy field rice-shrimp co-cultivation method using sulfate-reducing bacteria, which comprises the following steps: step 1, spraying diluted SRB bacterial agent propagation liquid in the cadmium-polluted paddy field to be treated, then making the bacterial agent fully contact with the soil in the form of rotary tillage, and adding water in the paddy field to a depth of 5-10 cm, wherein the cadmium content in the cadmium-polluted paddy field to be treated is 0.40-1.50 mg / kg, and the pH of the cadmium-polluted paddy field ranges from 5.5 to 6.5; step 2, monitoring the redox potential of the soil in the paddy field until the redox potential value of the soil is monitored to be below-150 mV within a preset time period, and then performing rice transplanting operation; step 3, after 10-15 days of rice transplanting, putting Australian freshwater lobster fry with a body length of 2-5 cm; and step 4, harvesting rice and lobsters at the mature stage of the rice respectively. The application aims to significantly reduce the cadmium content in rice and the availability of soil cadmium through the sulfate-reducing bacteria and rice-shrimp co-cultivation system.

According to the preparation method of the light filter material for deeply removing the total nitrogen and the total phosphorus, the foaming porous filter material is prepared by taking the steel slag, the zeolite, the lime and the like as main components, and the sludge is cultivated in the starting period, so that the tolerance of microorganisms to sulfide toxicity is improved; sulfur and cultured strains of autotrophic thiobacillus denitrificans and sulfate reducing bacteria are solidified on the porous filter material through a biological embedding technical means, so that autotrophic denitrification of elemental sulfur and reduction of sulfate radicals are realized. The porous structure of the light filter material is beneficial to transmission of substances such as nitrogen and phosphorus, the surface of the filter material can provide conditions for attachment and growth of microorganisms such as phosphorus-accumulating bacteria, nitrifying bacteria, thiobacillus denitrificans and sulfate reducing bacteria, the high porosity and large specific surface area of the filter material can bring a large microorganism attachment amount, and the huge specific surface area is beneficial to growth of a biological membrane, so that the biological membrane can be effectively degraded. The filter material is subjected to combined treatment through a solid physicochemical adsorption method and a microbiological method, the pollutant removal capacity is high, and the effect is remarkable.

This invention discloses a method for adsorbing and degrading high-concentration tetracycline in high-salt wastewater using activated sludge. The method comprises two steps. The first step uses a continuous influent process in a completely mixed reactor to selectively acclimate heterotrophic activated sludge with sulfate-reducing bacteria (SRB) as the primary functional bacteria. Adding influent cultured with low tetracycline concentrations enhances the tetracycline tolerance of the microorganisms and promotes the secretion of extracellular polymeric substances (EPS) by the bacteria. The EPS adsorbs and degrades the tetracycline. By adjusting the pH value in stages, the horizontal transfer of antibiotic resistance genes is effectively inhibited. The second step utilizes the successfully acclimatized anaerobic activated sludge to efficiently degrade the high-concentration tetracycline. This invention successfully achieves effective treatment of high-concentration tetracycline wastewater generated by industries such as marine aquaculture, while simultaneously curbing the spread of resistance genes, resulting in significant environmental and economic benefits.