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124 results about "Desulfobacter sp." patented technology

Nutrient source immobilized sulfate reducing bacterium globules as well as preparation method and application thereof

The invention relates to a preparation method and application of nutrient source immobilized sulfate reducing bacterium globules. Immobilized globules take polyvinyl alcohol and sodium alginate as carriers, a saturated boric acid calcium chloride solution as a cross-linking agent and sodium lactate as a nutrient source. The preparation method comprises the specific steps: firstly, dissolving the polyvinyl alcohol and the sodium alginate in a water bath at a constant temperature; then, adding active carbon, calcium carbonate, silicon dioxide and free bacterium liquid; sucking a mixed solution by an injector and dropwise adding the mixed solution into the saturated boric acid calcium chloride solution; crossly linking for 24h to form the globules; washing the globules with sterile physiological saline and putting the globules into a sodium lactate solution for 24h; after washing, freezing and drying to obtain the nutrient source immobilized sulfate reducing bacterium globules. In the application of the nutrient source immobilized sulfate reducing bacterium globules prepared by the invention, the nutrient source immobilized sulfate reducing bacterium globules have a good removing effect on four types of heavy metal including Cu, Zn, Pb and Cd in sediment of Xiang River; the treatment is stable and the leaching rate is low. The material is convenient to separate, can be repeatedly utilized and does not cause secondary pollution; the nutrient source immobilized sulfate reducing bacterium globules has good economic benefits and a wide application prospect.
Owner:HUNAN UNIV

Method for processing heavy metal wastewater

The invention relates to a removal method of heavy metal ion in wastewater, such as chrome, belonging to the technical field of wastewater treatment. The removal method is characterized in that nanometer FeSx is produced by culturing a compound bacteria comprising desulfovibrio Sp. CB1. 268, desulfotomaculum Sp. CB1.139, and desulfobacter Sp. CB1.168 in a culture medium comprising lactic acid proteinate, ferrite, sulfate, K, Mg, Ca, Cu, Mn, B, Si, and Mo under anaerobic or facultative, and alkaline condition at the temperature between 30 and 39 DEG C for thirty-six to 72 hours; the nanometer FeSx is dissociated into S<2-> and Fe<2+> under acidic condition, can reduce Cr<6+> to Cr<3+>, and generate unsolvable sedimentation of metal sulphides with Ni<2+>, Cu<2+>, Zn<2+>, and other elements; after the sedimentation is separated, the metal ions in the wastewater are removed. The removal method has the advantages of high removal efficiency and low cost.
Owner:CHENGDU INST OF BIOLOGY CHINESE ACAD OF S

Method and apparatus for removing sulfate in high-salt petrochemical wastewater

The invention relates to a removing method and a removing apparatus for sulfate in high-salt petrochemical wastewater, and belongs to the technical field of wastewater treatment. According to the present invention, in order to provide a method with characteristics of low cost, simpleness, convenience and high sulfate removal rate, and an apparatus thereof, according to the principle of conversion of sulfate into hydrogen sulfide with sulfate reducing bacteria, residual sludge produced by a high-salt petrochemical wastewater treatment system precipitation tank is subjected to ultrasonic wave degradation through an ultrasonic wave treatment, the obtained sludge or sludge supernatant and high-salt petrochemical wastewater are mixed, and then enter an anoxic tank or anaerobic tank so as to provide a carbon source for the sulfate reducing bacteria, and a blowing removing manner under a N2 solution is adopted to remove the hydrogen sulfide so as to reduce inhibition effects of hydrogen sulfide and other sulfides on the sulfate reducing bacteria; and the sulfate reduction can be effectively promoted, the sulfate removal rate can be increased, the calcium sulfate scaling tendency can be reduced, the maintenance period and the service life of the equipment can be prolonged, and the system stability is increased.
Owner:CHINA PETROLEUM & CHEM CORP

Wastewater treatment method for synchronously removing organic matters, sulfate radicals, heavy metals and total nitrogen

The invention discloses a wastewater treatment method for synchronously removing organic matters, sulfate radicals, heavy metals and total nitrogen. The method comprises the following steps that: production wastewater enters an anaerobic tank, organic matters are used as electron donors, and SO4<2-> in the production wastewater is reduced by sulfate reducing bacteria; the wastewater flows into thereaction tank again, S2- reacts with heavy metal ions in the wastewater to generate insoluble metal sulfide, and a coagulation reaction is carried out; the wastewater flows into a sedimentation tankfor solid-liquid separation, and metal sulfide in the wastewater is removed through sedimentation; the wastewater is flown into an anoxic tank, wherein thiobacillus denitrificans performs autotrophicdenitrification nitrogen removal by utilizing residual S2-, S and SO3<2->; and the wastewater flows into the aerobic tank, and aerobic denitrifying bacteria in the wastewater perform aerobic denitrification by utilizing residual organic matters in the wastewater to further remove nitrogen. According to the method, the energy consumption can be reduced by 30%-50%, the sludge generation amount can be reduced by 30%-40%, the manual operation amount can be reduced, and the method conforms to the development trend of treating waste with waste in future sewage treatment.
Owner:SOUTH CHINA UNIV OF TECH

Immobilized granules for treating acidic mine wastewater, and preparation and application methods thereof

The invention relates to immobilized granules for treating acidic mine wastewater. The immobilized granules for treating acidic mine wastewater comprise the following components in percentage by weight: 20-40% of sulfate reducing bacteria sludge, 5-10% of nano iron composite granular material, 5-15% of hydrotalcite / hydrotalcite roasted product, 5-9% of polyvinyl alcohol, 0.2-1.5% of sodium alginate, 5-20% of biomass material and 4.5-59.8% of distilled water. The preparation method comprises the following steps: adding the polyvinyl alcohol and sodium alginate into the distilled water, and swelling and stirring to form a bubble-free gel; adding the nano iron composite granular material and hydrotalcite / hydrotalcite roasted product into the gel, and cooling by stirring to form a cooled gel; and adding the sulfate reducing bacteria sludge, dropwisely adding the saturated boric acid solution while stirring, and crosslinking to obtain the immobilized granules. The application method comprises the following steps: activating the immobilized granules, putting in a permeable reaction wall, and treating the acidic mine wastewater. On the premise of lowering the cost, the immobilized granules effectively remove heavy metals and anionic pollutants in the acidic mine wastewater, and have obviously higher treatment efficiency.
Owner:LIAONING TECHNICAL UNIVERSITY

Molecular detection method for desulfovibro

The invention discloses a molecular detection method for desulfovibro. The nucleotide sequences of the specific primer pair adopted by the detect method are SEQ ID No.1 and SEQ ID No.2. The detection method comprises the following steps: (1) establishing a standard curve and a linear equation for quantitative detection of desulfovibro; (2) pretreating a sample; (3) taking the pre-treated sample as a mold plate, establishing a PCR reaction system by utilizing the primer pair, and carrying out PCR amplification; (4) carrying out agarose gel electrophoresis detection; (5) analyzing the electrophoresis result by utilizing a gel imaging system to obtain a relative strip density value, and quantitatively determining the concentration of the desulfovibro by looking up the standard curve and the linear equation. The quantitative molecular detection method has the advantages that the time consumption is low; the operation is simple; the sensitivity is high; the test charge is low; the detection effect is broad-spectrum; desulfovibro in various samples can be quantitatively detected.
Owner:北京世纪金道石油技术开发有限公司 +1

Oil field water biological treatment agent as well as preparation method and use method thereof

The invention relates to the technical field of sewage treatment agents, and relates to an oil field water biological treatment agent as well as a preparation method and a use method thereof. The oil field water biological treatment agent is obtained according to the preparation method which comprises the following step: sufficiently mixing a required quantity of denitrification and nitrification bacteria solutions, nitrate, nitrite, biquaternary ammonium salt sludge strippers, oil field sewage, potassium permanganate, potassium dichromate and water to obtain the oil field water biological treatment agent. The oil field water biological treatment agent disclosed by the invention can sufficiently exert the richness and effectiveness of indigenous beneficial microorganisms by being prepared by adopting oil field sewage, comprehensively inhibit the activity and growth of sulphate reducing bacteria and control the generation of hydrogen sulfide by enabling the indigenous beneficial microorganisms to compete with the residual sulphate reducing bacteria by virtue of a higher advantage, fundamentally reduce the problem of secondary hydrogen sulfide caused by the sulphate reducing bacteria by inhibiting the growing environment of the sulphate reducing bacteria and changing the generation way of corrosion, prevent the factor of increasing oil field corrosion and inhibit the cost.
Owner:KARAMAY JINSHAN PETROCHEMICAL LTD CO

Method for preparing strain and microbial agent for heavy metal contaminated water treatment

The invention discloses a method for preparing a strain and a microbial agent for heavy metal contaminated water treatment. The strain is a sulfate reducing bacterium Desulfovibrio oxamicusw, has a preservation number of CGMCC 9548, and is named D. oxamicusw CGMCC 9548. The D. oxamicusw CGMCC 9548 has a removal rate on SO4<2->, Pb, Cd, As and W to be over 80 percent, so that heavy metals in a water body can be removed, and the pH value of the water body can be increased. The strain is applied to heavy metal water contamination treatment of industrial and acid mine wastewater. The method provides a developed fermentation method to large-scale fermentation production of D. oxamicusw CGMCC 9548 strain microbial agent, and the product strain density is over 1012. The microbial agent can be used for removing heavy metals in a water body and increasing the pH value of the water body, and can be applied to heavy metal water contamination treatment of industrial and acid mine wastewater.
Owner:POWERCHINA ZHONGNAN ENG +1

Preparation method for high-efficiency oilfield bactericide

ActiveCN103340202AHas dual-effect bactericidal propertiesAddressing drug resistanceBiocideDisinfectantsPolyolReverse osmosis
A preparation method for a high-efficiency oilfield bactericide comprises the following preparing steps: adding a solvent into a reaction kettle, starting to heat, and adding polyol or / and polyphenol to the reaction kettle; controlling the temperature of 50 DEG C or less and dropwise adding tertiary amine, after finishing addition, stirring fully, and holding the temperature for 10 min; raising the temperature, dropwise adding epoxy chloropropane, after finishing addition, and holding the temperature for reaction; and adding reverse osmosis water to adjust an active matter content. The bactericide has the special advantages that: the prepared oilfield dedicated bactericide has double-effect bactericidal performance; the bactericide is an updating product of an oilfield bactericide SRB, and can effectively solve a drug resistance problem of sulfate reducing bacteria; and the bactericide has good compatibility, and can effectively exterminate and inhibit the oilfield sulfate reducing bacteria. The bactericide has a significant inhibiting effect on bacterial breeding in oilfield water injection and oil extraction production systems, and is especially suitable for polymer-containing oil extraction wastewater treatment and reinjection systems.
Owner:山东泰和科技股份有限公司

Oligomeric quaternary ammonium salt bactericide and preparation method thereof

The invention relates to an oligomeric quaternary ammonium salt bactericide and a preparation method thereof. Ester p-toluenesulfonate-based gemini quaternary ammonium salt, guanidine hydrochloride and sodium alkoxide are added into solvent, stirred and heated to 120 DEG C to 180 DEG C, heating is stopped after 10 to 48 hours of reaction, so that a crude product is obtained, and after post-treatment, the oligomeric quaternary ammonium salt bactericide is obtained; and the substance amount ratio of the ester p-toluenesulfonate-based gemini quaternary ammonium salt, the guanidine hydrochloride and the sodium alkoxide is (0.008 to 0.040):0.008:0.008. The gemini quaternary ammonium salt is introduced to a plurality of active sites, eight active sites are contained in the structure of the oligomeric quaternary ammonium salt bactericide, the oligomeric quaternary ammonium salt bactericide can show good water solubility and surface activity in aqueous solution, and an important role is played in the process of oligomeric quaternary ammonium salt preparation. A bacteria killing test on three types of bacteria, i.e. sulfate reducing bacteria, iron bacteria and saprophytic bacteria, indicates that the oligomeric quaternary ammonium salt bactericide has a good bactericidal effect.
Owner:SHAANXI UNIV OF SCI & TECH

Sulfate reducing bacteria corrosion-resistant oil well pipe and production method thereof

The invention relates to a sulfate reducing bacteria corrosion-resistant oil well pipe and a production method thereof. The sulfate reducing bacteria corrosion-resistant oil well pipe consists of the following chemical components in percentage by weight: 0.12 percent or less of C; 0.15 to 0.45 percent of Si; 0.7 percent or less of Mn; 8.5 to 25 percent of Cr; 0.15 to 2.5 percent of Cu; 0.5 to 3.5 percent of Al; and the balance of Fe and inevitable impurities. The production method for the sulfate reducing bacteria corrosion-resistant oil well pipe includes the following steps: the chemical components are smelted and continuously cast into a round billet or cast into a square billet which is then rolled into a round billet; after hot rolling, a seamless steel pipe is produced; high-temperature solution heat treatment is carried out, the heating temperature is 950 DEG C to 1200 DEG C, and the temperature-keeping time is 30 to 120 minutes; the tempering temperature is 650 DEG C to 750 DEG C, and the tempering temperature-keeping time is 60 to 90 minutes; sizing and straightening are carried out, the hot straightening temperature is higher than 450 DEG C, and therefore the oil well pipe is produced. The oil well pipe produced by the method can effectively resist SRB (sulfate reducing bacteria) corrosion in external environment, moreover, the yield strength of the oil well pipe is greater than 380MPa, thus meeting the requirement of J55 steel grade, and better meeting the requirements of production strings for water injection wells under harsh environment.
Owner:BAOSHAN IRON & STEEL CO LTD

Method for cooperatively treating arsenic-containing waste liquid by using sulfate reducing bacteria and lead-zinc smelting slag

The invention discloses a method for cooperatively treating arsenic-containing waste liquid by using sulfate reducing bacteria and lead-zinc smelting slag. The method comprises the following steps: (1) stirring and mixing the lead-zinc smelting slag and the arsenic-containing waste liquid in an anaerobic environment and conducting reacting to obtain to-be-treated waste liquid; and (2) adding sulfate reducing bacteria into the to-be-treated waste liquid obtained in the step (1), adding sodium lactate, and carrying out microbiological treatment, so as to synergistically treat the arsenic-containing waste liquid by the sulfate reducing bacteria and the lead-zinc smelting slag. According to the method for cooperatively treating the arsenic-containing waste liquid through the sulfate reducing bacteria and the lead-zinc smelting slag, the sulfate reducing bacteria and the lead-zinc smelting slag cooperate with each other, the removal rate of arsenic in the arsenic-containing waste liquid is jointly increased, and the removal rate of arsenic in the arsenic-containing waste liquid reaches 99.80% or above.
Owner:CENT SOUTH UNIV

Method and device for improving beneficiability of niobium mineral in niobium-containing tailings

The invention provides a method and a device for improving beneficiability of a niobium mineral in niobium-containing tailings. The method comprises the following steps: spraying a culture solution containing ferrous oxide thiobacilli to a niobium-containing tailing heap, wherein the ferrous oxide thiobacilli can oxidize Fe<2+> on the surface of the niobium mineral in the niobium-containing tailings into Fe<3+> so as to be dissolved out, thereby changing the category and density of particles on the surface of the mineral and improving the floatability difference between the niobium mineral and other minerals in the niobium-containing tailings; reducing Fe<3+> on the surface of hematite or magnetite into Fe<2+> ions so as to be dissolved out by using iron-reducing bacteria; meanwhile enabling Fe<2+> to rapidly form a sulfide precipitate on the surface of hematite or magnetite in the presence of sulfate reducing bacteria or Na2S, thereby improving the floatation difference between the hematite or magnetite and the niobium mineral. By using the method, the recovery rate and grade of the niobium mineral in the niobium-containing tailings can be improved, meanwhile the environmental pollution and the use of chemical agents such as an inhibitor and a regulator in a floatation process can be reduced; the method has high social benefits.
Owner:INNER MONGOLIA UNIV OF SCI & TECH

Magnesium alloy microbial corrosion method based on solid medium

InactiveCN101226136AObserve the growth formCorrosion results are intuitiveWeather/light/corrosion resistanceMicrobiological testing/measurementMicroorganismSulfate-reducing bacteria
The invention relates to a sulfate reducing bacteria microbe corrosion method of magnesium alloy, for resolving the microbe corrosion problem of magnesium alloy and combining the microbe cultivation technique and microbe corrosion technique of solid culture medium to provide a novel magnesium alloy microbe corrosion method based on solid culture medium. The method comprises (1) processing and disinfecting sample, (2) arranging and disinfecting a solid culture medium, (3) cultivating and activating bacterial, (4) corroding paster. The invention optimizes the components of electrolyte to highlight the bacterial performance in the corrosion process of magnesium alloy, and compares the electrode surface corrosion morphology with bacterial and without bacterial, to provide the reference for magnesium alloy microbe corrosion mechanism research. The inventive method is suitable for magnesium alloy microbe corrosion research.
Owner:JILIN UNIV

Method for regenerating and recycling waste leaching liquid used for heavy metal contaminated soil

The invention discloses a method for regenerating and recycling waste leaching liquid used for heavy metal contaminated soil. The method comprises the steps that a carrier is flushed by water, soakedin acid overnight, flushed to be neutral and dried, domesticated sulfate reducing bacteria is inoculated in a culture medium and cultured for 2-3 days, culturing liquid completely becomes black, the dried carrier is placed in the culturnign liquid, the mass ratio of sulfate reducing bacteria liquid and the carrier is (10-20):1, the stirring reaction is conducted for 1-2 days in a culturing box atthe constant temperature of 35 DEG C or 39 DEG C, solid-liquid separation is conducted; after a solid sample is dried, a waste leaching liquid regenerant is obtained. The method can regenerate and recycle the chemical waste leaching liquid and has the advantages that the operation is simple, the cost is low, and the method is suitable for using and popularization.
Owner:TAIYUAN UNIVERSITY OF SCIENCE AND TECHNOLOGY

Method for producing graphene from biological cathode through reduction and removing sulfate

The invention relates to a method for producing graphene from a biological cathode through reduction and removing sulfate and belongs to the field of bioelectrochemistry systems. The system is characterized in that a microorganism electrolytic tank in the field of energy production and wastewater treatment is applied to in-situ production of graphene and synergetic treatment on sulfate wastewaterfor a first time, and by domesticating a certain microorganism amount of sulfate reducting bacteria, and inoculating the sulfate reducting bacteria with a cathode chamber, a stable autotrophic biological membrane is formed on a cathode electrode. Sulfate wastewater of a certain concentration is manually prepared, in addition, graphene oxide of a certain concentration is added and is ultrasonicallydispersed into the sulfate wastewater, and then a cathode liquid is prepared. A reasonable hydraulic retention time is determined, a substrate is replaced when an operation cycle is completed, graphene in an effluent of the cathode chamber is effectively collected, and in addition, a certain amount of sulfate can be removed.
Owner:SUN YAT SEN UNIV

Biological activated lignite synergistic sulfate reducing bacteria sludge immobilized particles as well as preparation method and application thereof

The invention provides biological activated lignite synergistic sulfate reducing bacteria sludge immobilized particles and a preparation method and application thereof. The immobilized particles can be used for reducing, adsorbing and removing various metal cations and anions in acid mine wastewater, and the preparation method of the immobilized particles comprises the following steps: 1) polyvinyl alcohol and sodium alginate are added into water, and swelling, heating and stirring are conducted until bubble-free gel is formed; 2) lignite is added into the gel obtained in the step 1), and uniform stirring and cooling are conducted; 3) rhodopseudomonas sphaeroides liquid and sulfate reducing bacteria sludge are added into the gel mixture obtained in the step 2), the mixture is uniformly stirred, and particles are prepared from the uniformly stirred mixture; 4) the particles obtained in the step 3) are dropwise added into a CaCl2-containing saturated boric acid aqueous solution with the pH value of 5.0-7.0, and crosslinking is carried out under stirring; and then normal saline is used for washing, and moisture on the surface is adsorbed to obtain the biological activated lignite synergistic sulfate reducing bacteria sludge immobilized particles.
Owner:SHENHUA SHENDONG COAL GRP

Protective coating of pipe wall, electroplating method and application

The invention provides a protective coating corroded by sulfate reducing bacteria, an electroplating method and application. The protective coating is a three-layer composite coating; the composite coating comprises bottom-layer semi-bright nickel, middle-layer bright copper and outer-layer bright nickel; the potential of the bottom-layer semi-bright nickel is higher than that of the middle-layerbright copper by 120mV or above, and the potential of the middle-layer bright copper is higher than that of the outer-layer bright nickel by 60-100 mV; the electroplating method adopts a flowing electroplating mode; and an adding mode of an additive of electroplating liquid is to add the additive at a liquid outlet of a plating tank with electroplating current as metering reference. The protectivecoating is perfect in technological process, the additive is reasonably matched and used, multilayer corrosion prevention is performed on the inner walls of oil pipes and sleeves, and the corrosion of the sulfate reducing bacteria to pipe matrix can be effectively reduced.
Owner:KARAMAY SHUANGXIN ANTICORROSION TECH

Black and odorous sludge treatment method

The invention discloses a black and odorous sludge treatment method, and belongs to the technical field of sewage treatment. According to the method, dredged sediment in the black and odorous river and lake control process is used as treatment objects; the sludge is put into a biological acidification leaching pool; black metal sulfides in the sludge are oxidized into sulfate radicals and metal ions in the leaching pool; organic sulfur is oxidized and decomposed; biological leaching supernatant fluid is introduced into a sulphate reducing bacterium anaerobic reactor; the sulfate radicals are reduced into sulfur ions; ferric salt or ferrous powder is added into outlet water of the reactor, so that the sulfur ions are precipitated in a form such as ferric sulfide. The method can be used forperforming black and odor removal on the sulfur-containing ingredients in the dredging sludge, and can also be used for removing and recovering heavy metal in the sludge.
Owner:KUNMING UNIV OF SCI & TECH

Device for in-situ remediation of cadmium and lead contaminated farmland soil through sulfate reduction system driven by microorganism electrochemistry and method thereof

ActiveCN111054740AReduce lead toxicityWill not cause significant changes in physical and chemical propertiesContaminated soil reclamationElectrolysisEdaphic
The invention discloses a device for in-situ remediation of cadmium and lead contaminated farmland soil by microbial electrochemical enhanced sulfate reduction and a method thereof. According to the method, a sulfate reduction reactor is used for generating the sulfur-containing (S<2->) water system passivator, the passivator can be used for in-situ remediation of cadmium and lead polluted farmland soil, and cadmium and lead are converted into forms which are difficult to absorb by crops. A double-chamber sulfate reduction reactor is constructed, current is applied to promote hydrogen production reaction of a cathode chamber, and autotrophic sulfate reducing bacteria in the cathode chamber can reduce sulfate into S <2-> by utilizing hydrogen and an inorganic carbon source generated by water electrolysis of an electrode. After the passivator containing S<2-> is mixed with soil, the toxicity of cadmium and lead in farmland soil can be reduced. The liquid passivator is used for restoringthe cadmium and lead polluted farmland soil, the physicochemical property of the soil cannot be obviously changed, and the device and the method have obvious advantages compared with the mode of restoring the heavy metal polluted soil through quick lime, biochar and the like.
Owner:ZHEJIANG UNIV

Gas stripping internal circulation type treatment process and system for treating acid mine wastewater by utilizing SRB

InactiveCN110482795AAvoid reduction efficiency impactRapid alkali productionWaste water treatment from quariesWater contaminantsSulfate-reducing bacteriaNutrient solution
The invention discloses a gas stripping internal circulation type treatment process and a gas stripping internal circulation type treatment system for treating acid mine wastewater by utilizing SRB. The process comprises the steps of stripping a reaction zone to a reduction atmosphere, carrying out a biochemical reduction sulfur production reaction, carrying out a first precipitation reaction andcarrying out a second precipitation reaction; the system comprises a first precipitation reaction device, a second precipitation reaction device, a gas stripping reaction device and a nutrient solution storage device. According to the method, the influence of multiple (acid, heavy metal and sulfide) toxicity inhibition on the sulfate reduction efficiency can be avoided; in addition, sulfate reducing bacteria in the gas stripping reaction device are high in activity and quick in alkali production, the pH of the acid mine wastewater in the second precipitation reaction device can be directly neutralized to be neutral, pretreatment of the acid mine wastewater is not needed, and the cost is reduced; the sulfate reduction rate in the gas stripping reaction device is high, the sulfide yield is high, heavy metal ions in the acid mine wastewater in the precipitation reaction device can be rapidly removed, and meanwhile valuable metal is recycled.
Owner:KUNMING UNIV OF SCI & TECH

Repairing method for remediation of antimony and zinc combined pollution and application

The invention discloses a remediation method for remediation of antimony and zinc combined pollution and application, and particularly relates to the field of soil remediation. The remediation method comprises the steps of enrichment of sulfate reducing bacteria, curing of the sulfate reducing flora on the Sb (III) and the Zn (II) and acute toxicity detection. When the microbial method provided by the invention is used for treating antimony-zinc combined pollution, the cost is low, the efficiency is high, and harmful pollutants in the environment can be treated in situ, so that the sustainable development of the environment is realized. The microbial metabolism capacity is high, the specific surface area is large, and the surface is charged, so that the microbial can repair heavy metal pollution through cell metabolism, biological adsorption, precipitation and redox. The sulfate reducing flora provided by the invention can be used for simultaneously converting Sb (III) and Zn (II) into sulfide precipitates and converting free forms of the Sb (III) and the Zn (II) into insoluble precipitates, so that the environmental pollution degree is reduced, a bioremediation material is provided for remediation of heavy metal pollution of mines, and the sulfate reducing flora has a very good industrial application prospect.
Owner:CHINA UNIV OF GEOSCIENCES (BEIJING)

Test apparatus for research on laws of influence of sulfate reducing bacteria on stress corrosion cracking of metal

The invention discloses a test apparatus for research on laws of influence of sulfate reducing bacteria on stress corrosion cracking of metal. The integral test apparatus comprises a stress corrosion test machine, an electrochemical workstation, a nitrogen cylinder, a constant-temperature water bath and a control system. The stress corrosion test machine comprises a power device, a transmission gear, a test specimen fixture, a solution box and a test specimen surface detector, and interfaces can be provided for other devices by the stress corrosion test machine. Oxygen can be removed by nitrogen in stress corrosion test procedures, so that anaerobic environments can be provided for the sulfate reducing bacteria. Bent test specimens can further deform by the aid of the power device and the transmission gear, so that specific stress can be applied. Stress corrosion can occur on the bent test specimens under the joint effects of the external stress and sulfate reducing bacterial corrosion solution. Corrosion signals can be monitored by the aid of the electrochemical workstation in real time, and nucleation and propagation conditions of surface crack can be periodically observed by the aid of the test specimen surface detector. The test apparatus has the advantages that test conditions can be precisely controlled, the test apparatus is high in automation degree, and conditions can be provided for research on the influence of the sulfate reducing bacteria on the stress corrosion cracking of the metal.
Owner:LIAONING UNIVERSITY OF PETROLEUM AND CHEMICAL TECHNOLOGY +1

Method of biologically synthesizing ZnxCd1-xS quantum dots by using zinc-cadmium wastewater as metal source

The invention relates to a method of preparing various different fluorescent Znx-Cd1-xS quantum dots having uniform particle size simply and efficiently in batch through zinc-cadmium wastewater via sulfate-reducing bacteria, and belongs to the technical field of green techniques. Quantum dots about 7 nm in uniform particle size and having good dispersity are prepared by adding sulfate-reducing bacteria; various Znx-Cd1-xS quantum dots with different light-emitting peaks are prepared according to wastewaters having different Zn-Cd concentration ratios. When the Zn-Cd concentration ratio in a solution is 3:1 to 20:1, the prepared ZnxCd1-xS quantum dots have photoluminescent spectrum from red light to blue light when excited at 365 nm, and have photoluminescent peaks at 470-590 nm. The different fluorescent ZnxCd1-xS ternary quantum dots are synthesized for the first time herein through a microbiological method; the problems of the prior art, such as high zinc-cadmium wastewater yield, high toxicity and low recycling value, are solved.
Owner:BEIJING INSTITUTE OF TECHNOLOGYGY

Method for treating oil-field waste-water refilling by catalytic oxygenation-desorption deaerization UV sterilization

The invention provides a method for processing and recycling oil-field exploitation waste water, making air oxidation and catalytic oxidation on the waste water, making catalytic oxidation and air oxidation on volatile phenol, sulfide, Fe2+, etc, on more moderate reaction conditions, and modifying them; filtering out deposits, removing oxygen from the filtrate by low-temperature desorption and obtaining oxygen free water, and finally killing sulfate reducing bacterium, saprophyte and iron bacterium by UV sterilizing processing. And the test shows that it can process polymer-containing waste water in oil field to make the waste water directly reach water reinjection requirements, having low operating expenses.
Owner:NANKAI UNIV

Sludge compost heavy metal passivation microbial agent as well as production method and application thereof

The invention provides a sludge compost heavy metal passivation microbial agent as well as a production method and application thereof. The passivation microbial agent comprises the following components in parts by weight: 10-16% of thermophilic sulfate reducing bacteria, and 4-6% of bacillus, with the balance being modified charcoal. The modified charcoal comprises 90 to 95 percent of charcoal and 5 to 10 percent of nano silicon dioxide loaded in pore channels of the charcoal; the average pore diameter of the modified charcoal is 6 to 9 nm; the specific surface area is 422-495 m<2> / g. In thesludge compost heavy metal passivation microbial agent obtained by the scheme, the fixation rate of microorganisms is 80-85%, the content of active heavy metal Cr is reduced by 85% or above, the amount of active Cu is reduced by 87% or above, and the amount of active Cd is reduced by 84% or above.
Owner:山西宁邦生物科技有限公司

Aerobic granular sludge rapid culture and stabilization method based on quorum sensing signal molecule synthesis

The invention discloses an aerobic granular sludge rapid culture and stabilization method based on quorum sensing signal molecule synthesis, and relates to the technical field of activated sludge culture. According to the method, aerobic activated sludge is used as seed sludge so that the formation of aerobic granular sludge is accelerated, and the granulation time is shortened; sulfate is added into inlet water of an aeration tank, and aerobic bacteria and sulfate reducing bacteria in activated sludge are symbiotic by utilizing an up-regulation action mechanism of sulfate on synthesis of quorum sensing signal molecule homoserine lactones (AHLs), so that the stability of the core region in granular sludge is maintained; and thus, the stability of the aerobic granular sludge in the long-term operation process is enhanced, and the pollutant removal capacity of the sludge in the earlier stage of granulation is improved.
Owner:华润环保发展有限公司

Oilfield water biological treatment agent and its preparation method and use method

The invention relates to the technical field of sewage treatment agents, and is an oilfield water biological treatment agent and its preparation method and use method. The oilfield water biological treatment agent is obtained by the following preparation method, and the required amount of denitrifying and nitrifying bacteria liquid, nitrate, nitrite, double quaternary ammonium salt type slime stripping agent, oilfield sewage, potassium permanganate, Potassium dichromate and water are fully mixed to obtain oilfield water biological treatment agent. The present invention adopts oilfield sewage to prepare oilfield water biological treatment agent, Can give full play to the richness and utility of indigenous beneficial microorganisms, Make it compete with the remaining sulfate-reducing bacteria with a greater advantage, thereby comprehensively inhibiting the activity and growth of sulfate-reducing bacteria, controlling the production of hydrogen sulfide, and fundamentally inhibiting the growth environment of sulfate-reducing bacteria and changing the path of corrosion Reduces secondary hydrogen sulfide problems caused by sulfate-reducing bacteria while being able to Avoiding factors that increase oilfield corrosion and containment costs 。
Owner:KARAMAY JINSHAN PETROCHEMICAL LTD CO

Thermophilic bio-trickling gas-liquid two-phase device and application thereof in removal of heavy metals in flue gas

The invention belongs to the technical field of environmental protection, and particularly relates to a thermophilic bio-trickling gas-liquid two-phase device and application thereof in removal of heavy metals in flue gas. The thermophilic bio-trickling gas-liquid two-phase device comprises a thermophilic bio-trickling device unit and an AA/O unit, the thermophilic bio-trickling device is internally provided with a three-stage filler layer (attached with a biological membrane), and high-temperature-resistant special-effect denitrifying bacteria and sulfate reducing bacteria are added to the biological membrane and are used for converting and stabilizing heavy metals in a gas phase. The AA/O unit is composed of an anaerobic tank, an anoxic tank, an aerobic tank and a sedimentation tank, anddenitrifying bacteria, devulcanizing bacteria and sulfurizing bacteria are added to convert and stabilize heavy metals in a liquid phase. The thermophilic bio-trickling gas-liquid two-phase device can be used for removing heavy metals in flue gas. The method for efficiently removing heavy metals in flue gas based on the device can overcome the defects that an adsorbent in an adsorption method inan existing heavy metal flue gas treatment technology is easy to saturate, secondary pollution such as hazardous wastes is generated, and the operation cost is relatively high.
Owner:SUN YAT SEN UNIV

Sulfur autotrophic denitrification advanced denitrogenation process and device

The invention relates to a sulfur autotrophic denitrification advanced denitrogenation device using sulfate in water as a sulfur source. The device comprises a filter tower. Secondary effluent to be treated enters from the lower part of the filter tower, and is treated and then discharged from the upper part of the filter tower. The filter tower is also provided with an exhaust port. The filter tower comprises a sulfate reduction area located at the lower part and a sulfur autotrophic denitrification area located at the upper part. A solid slow-release carbon source feeding hole is formed between the sulfate reduction area and the sulfur autotrophic denitrification area, and a slow-release carbon source can be supplemented to the sulfate reduction area through the solid slow-release carbon source feeding hole. The sulfate reduction area is filled with a filter material, and a sulfate reducing bacteria biological membrane is hung on the filter material. The sulfur autotrophic denitrification area is filled with a filter material, and a sulfur autotrophic denitrifying bacterium biological membrane is hung on the filter material. According to the invention, with sulfate contained in wastewater as an indirect nutrition source, sulfur autotrophic denitrification advanced denitrogenation is carried out under the condition of no additional sulfur source, so that resources are saved, the utilization rate of the sulfur source is maximized, and the operation cost is reduced. The process and the device are especially suitable for treating secondary effluent with low C/N ratio.
Owner:HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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