79 results about "Desulfobacter sp." patented technology

The invention relates to acid-resistant sulfate reducing bacteria and application thereof, and belongs to the technical field of microorganisms. The acid-resistant sulfate reducing bacterium disclosed by the invention is Desulfovibrio desulfuricans QY411, and is preserved in the Guangdong Microbial Culture Collection Center on March 14, 2024, and the preservation number of the acid-resistant sulfate reducing bacterium is GDMCC No: 64412. The acid-resistant sulfate reducing bacterium provided by the invention can survive in an environment with the pH value of 4, and can be applied to ecological reconstruction of acid tailings and waste dump soil, the pH values of mining wasteland soil and surface water after ecological reconstruction are remarkably increased, the concentration of SO4 < 2-> in the soil is remarkably reduced, the acidification process in the mining wasteland soil is effectively inhibited, and the acid-resistant sulfate reducing bacterium has the advantages of being safe and reliable. Therefore, the acidification process in the mining wasteland is effectively controlled. Meanwhile, soil heavy metal pollution is reduced, and vegetation growth is recovered.

The invention discloses a lead-zinc mine ecological restoration method driven by combination of pennisetum purpureum and soil microorganisms. After soil in a to-be-repaired area of a mine is loosened and conditioned, pennisetum purpureum is planted, different microorganisms A and microorganisms B are introduced before and after the pennisetum purpureum is planted respectively, the microorganisms A are composed of metal reducing bacteria and sulfate reducing bacteria, the microorganisms B are composed of heavy metal tolerant microorganisms and symbiotic nitrogen-fixing bacteria, and the heavy metal tolerant microorganisms and the symbiotic nitrogen-fixing bacteria can be obtained through the method that the different microorganisms are introduced in sequence. Heavy metals in the mine soil are transformed and absorbed for multiple times, and ecological restoration of the mine is realized. Through the combined action of microorganisms and plants, accumulation of organic matter in soil can be promoted, the diversity and activity of soil microbial communities can be restored, finally, restoration of a soil ecological system is achieved, in the restoration process, the root system of the pennisetum purpureum can assist in enhancing the structure and permeability of the soil through symbiosis with the microorganisms, and the soil quality is improved. And a foundation is laid for future ecological restoration.

The invention discloses a method for preparing a waste incineration flue gas demercuration adsorbent by utilizing microorganism induced corrosion waste battery slag, which comprises the following steps: firstly, preparing an SRB (sulfate reducing bacteria) seed culture medium by mixing SRB and an anaerobic culture medium, and then adding sulfur-rich waste battery slag into the SRB seed culture medium for anaerobic reaction; and after the reaction is finished, centrifuging, washing with deionized water and drying to obtain amorphous iron sulfide (FeSx) generated by microorganism induced corrosion. According to the method, the sulfate reducing bacteria culture medium is used as the precursor solution, the waste battery slag is subjected to anaerobic modification to prepare the mercury removal adsorbent, on one hand, high-valued and resource utilization of the sulfur-rich waste battery slag is achieved, and on the other hand, the method capable of rapidly, accurately and simply preparing the high-activity amorphous metal sulfide adsorbent in a large-scale mode is explored. Meanwhile, the type of the flue gas demercuration adsorbent is also expanded.

The invention relates to the technical field of wastewater treatment, and provides a treatment device and method for treating high-concentration sulfate wastewater, and the device comprises a pretreatment unit, a bioreactor, a chemical precipitation unit and a solid-liquid separation unit; the pretreatment unit comprises a pH adjusting tank and a constant temperature device and is used for adjusting the pH value and the temperature of wastewater; the bioreactor is filled with a hydrogen sulfide adsorption filler layer and a sulfate reducing bacteria immobilization carrier; a stirring device and a calcium salt adding device are arranged in the chemical precipitation unit, and the chemical precipitation unit is connected with the bioreactor through an overflow pipe; the solid-liquid separation unit comprises an inclined plate sedimentation tank and a precision filter and is used for carrying out solid-liquid separation on the wastewater. According to the invention, two-stage cooperative processing is adopted, so that both efficiency and economy are improved; sludge reduction and resource recovery are carried out; modularized and intelligent design is carried out, operation is stable, and maintenance is easy.

The invention relates to the field of soil remediation and improvement, and discloses a protonated chitosan modified sustained-release material and a preparation method and application thereof.The protonated chitosan modified sustained-release material is of a core-shell structure, an inner core contains sulfate, and a shell layer contains protonated chitosan modified porous carbon; the raw materials for preparing the protonated chitosan modified porous carbon contain biomass. The protonated chitosan modified sustained-release material provided by the invention can continuously provide electron acceptors for indigenous sulfate reducing bacteria by continuously releasing sulfate radicals and stimulate the sulfate reducing bacteria to continuously utilize an organic carbon source in an environment for metabolic proliferation, so that the effects of degrading and stabilizing pollutants are achieved, and the protonated chitosan modified sustained-release material has a relatively good application value.

The application discloses a biological electrochemical auxiliary sulfur conversion promoting sulfur-based denitrification and sulfate emission reduction method and belongs to the field of water treatment. ‑ Nitrogen-containing wastewater enters an elemental sulfur denitrification system, and NO3 0 is reduced to N2 by sulfur autotrophic denitrifying bacteria, and elemental sulfur (S 2‑ ) is converted into SO4 2‑ . Effluent enters a cathode chamber of an electrochemical auxiliary sulfate reduction system, and hydrogen autotrophic sulfate reducing bacteria utilize cathode hydrogen production to reduce SO4 ‑ to HS 2‑ , and meanwhile, alkalinity produced by cathode hydrogen production neutralizes acidity produced in the denitrification process in the elemental sulfur denitrification system. ‑ HS 2‑ / supernatant is returned to the elemental sulfur denitrification system to react with S 0 to generate polysulfide (S n 2‑ ), S n 2‑ , which is used as an efficient electron donor to participate in denitrification, thereby significantly improving denitrification efficiency. The application does not need to add an organic carbon source, sludge production is small, high-concentration nitrogen-containing wastewater is efficiently treated, and the content of sulfate in effluent is simultaneously reduced, thereby realizing sulfur resource recycling, and the application is an economic and environment-friendly autotrophic denitrification method.

The invention discloses an antibacterial peptide induced by sulfate reducing bacteria and application of the antibacterial peptide, and belongs to the technical field of marine corrosion protection. The amino acid sequence of the SRB-induced antibacterial peptide is VAHKRLVVGTR, and the SRB-induced antibacterial peptide has efficient antibacterial activity on corrosive bacteria such as SRB and has the advantages of being environmentally friendly and not prone to inducing the bacteria to generate drug resistance, and the problems that an existing antibacterial peptide is insufficient in corrosive bacteria specificity, poor in environmental stability and the like are solved; meanwhile, peptide chains are short, synthesis is easy, and the production cost is low; the compound corrosion inhibition bactericide formed by combining the compound with a chemical bactericide can significantly improve the antibacterial and antiseptic effects, saves the dosage of the chemical bactericide, effectively solves the problems of bacterial drug resistance, high cost, environmental pollution and other pain points caused by abuse of the existing bactericide, and is suitable for the fields of marine microbial corrosion protection and the like.

The invention belongs to the technical field of stripping agents, and particularly relates to a slime stripping agent and a preparation method and application thereof. The slime stripping agent disclosed by the invention is prepared from dodecyl dimethyl benzyl amine bromide, dichlorophenol, isothiazolinone, polyoxyethylene alkylolamide, hydroxyethylidene diphosphonic acid, dimethylformamide and sodium dodecyl benzene sulfonate. Through the combined action of the specific components, the slime stripping agent has good stripping capacity and can rapidly permeate and disintegrate slime, dirt and biological membranes attached to the surface of equipment, the cleanliness and operation efficiency of the equipment are recovered, and equipment blockage and performance reduction caused by slime accumulation are effectively avoided; meanwhile, the high killing effect on sulfate reducing bacteria and nitrifying bacteria is achieved.

The present invention discloses a triazine ring-containing cationic Gemini surfactant having a structure represented by the following general formula (I): #imgabs0#, wherein m = 6 to 16, and n = 2 to 6. Leveraging the molecular structural characteristics of the triazine ring-containing surfactant and the effects of its quaternary ammonium salt functional groups, the present invention achieves high corrosion inhibition and sterilization efficiencies even at relatively low dosages. The surfactant can be used as a corrosion inhibitor, particularly for corrosion inhibition and sterilization of carbon steel in the presence of sulfate-reducing bacteria.

The invention discloses a method for adsorbing and degrading high-concentration tetracycline in high-salinity wastewater by using activated sludge, which comprises the following two steps of: 1, directionally domesticating heterotrophic active sludge which takes sulfate reducing bacteria (SRB) as main functional bacteria based on a completely mixed reactor in a continuous water feeding manner; water-inflowing culture microorganisms with low-concentration tetracycline are added, so that the tolerance to tetracycline is improved, bacteria are promoted to secrete an extracellular polymeric substance (EPS), and the tetracycline is adsorbed by utilizing the EPS and is promoted to be degraded. By adjusting the pH value in stages, the horizontal transfer of antibiotic resistance genes is effectively inhibited. And 2, completing efficient degradation of high-concentration tetracycline by using the successfully domesticated anaerobic activated sludge. The method successfully realizes effective treatment of high-concentration tetracycline wastewater generated in industries such as mariculture and the like, inhibits diffusion of resistance genes, and has remarkable environmental benefits and economic benefits.

The present application belongs to the technical field of environmental remediation and soil ecological engineering, and particularly relates to a modified iron biochar-humus-sulfate-reducing bacteria (SRB) composite system for realizing efficient immobilization and ecological remediation of cadmium (Cd 2+ ) in heavy metal contaminated soil. The system can enhance the stability of microbial colonies and has the characteristics of physical and biological synergistic passivation, thereby realizing long-term stabilization and ecological remediation of heavy metals.

The invention discloses a microbial flora for treating sulfate wastewater and a preparation method of the microbial flora. Comprising the following microbial treatment flora components: 10-35% of biphasic photosynthetic bacteria, 5-10% of sulfate reducing bacteria, 5-30% of bifidobacterium, 3-8% of desulfurization vibrio, 5-25% of mesophilic actinomycetes, 2-5% of methanogens, 10-30% of sub-digestion bacteria, 2-6% of acidithiobacillus acidophilus, 12-30% of bacillus megatherium, 1-4% of iron-oxidizing bacteria, 1-5% of denitrifying bacteria and 1-3% of phosphorus-accumulating bacteria. According to the method, sulfate is efficiently removed, the removal rate of SO4 < 2-> is larger than or equal to 85%, the H2S output is reduced by 70% or above, multi-pollutant cooperative treatment of odor pollution is avoided, the removal rate of COD is larger than or equal to 60%, the removal rates of total nitrogen and total phosphorus are larger than or equal to 50% and 40% respectively, the comprehensive discharge standard is met, the environmental adaptability is high, the salt tolerance NaCl is smaller than or equal to 5%, the temperature fluctuation resistance is 20-40 DEG C, and the method is suitable for the complex environment of industrial wastewater, low in cost and suitable for industrial production. Extra chemical agents are not needed, strains can be bred autonomously, and the operation cost is reduced by 40% or above compared with a traditional method.

The application provides a wastewater treatment system and method for anaerobic coupling and electric stimulation synergistic bacteria-algae desulfurization, which comprises a primary anaerobic reactor and a secondary anaerobic reactor; the primary anaerobic reactor comprises an anaerobic fluidized state zone and a desulfurization reaction zone, the anaerobic fluidized state zone is filled with a filler carrier, the sulfate in the wastewater is converted into sulfide by sulfate-reducing bacteria attached to the filler carrier in the anaerobic fluidized state zone, the desulfurization reaction zone is filled with microalgae and desulfurization bacteria, oxygen is generated by photosynthesis of the microalgae in the desulfurization reaction zone, the oxygen is used by the desulfurization bacteria and the sulfide in the wastewater is converted into sulfur at the same time, the effluent outlet of the desulfurization reaction zone is connected with the secondary anaerobic reactor, and the organic matter in the wastewater is converted into methane in the secondary anaerobic reactor. The primary anaerobic reactor is used as a desulfurization process, the wastewater is recycled by desulfurization to avoid inhibition of anaerobic microorganisms in the downstream reactor by sulfide, and the wastewater enters the secondary anaerobic reactor again to degrade organic pollutants, so that the biodegradability of the wastewater is improved.

The invention relates to a method for in-situ remediation of hexavalent chromium contaminated soil and application of the method, according to the method, a nutritional additive solution and a sulfate reduction biological agent are added into the hexavalent chromium contaminated soil, and a nutritional additive contains organic acid salt. According to the method, the organic acid salt serves as a chelating agent to enable insoluble hexavalent chromium to be dissolved out of soil particles, meanwhile, the organic acid salt serves as a nutrition additive to promote reproduction and growth of sulfate reducing bacteria, sulfate is reduced into negative bivalent sulfur through the sulfate reducing bacteria, and the dissolved hexavalent chromium is reduced into trivalent chromium through the negative bivalent sulfur; finally, the purpose of treating the hexavalent chromium contaminated soil is achieved.

The invention discloses a device and a method for detecting the corrosion resistance of a cementing material by simulating a dynamic seawater impact environment, the device combines the dissolved oxygen regulation (2-8mg / L) of a corrosive medium adding system with the coupling of sulfate reducing bacteria (10-10CFU / mL) through the cooperative regulation of a flow making pump, a wave generator and a guide plate (the concentration deviation is less than or equal to + / -0.05%, and the temperature uniformity is less than or equal to + / -0.3 DEG C); chemical-biological-mechanical three-factor simulation is realized; the test piece fixing frame is of an electric rotating structure (0.5-2r / min), and is matched with dynamic impact to ensure that the test piece is uniformly impacted. According to the method, a corrosion rate curve (R is larger than or equal to 0.95) is fitted by presetting a marine environment template, closed-loop feedback control and an emergency pressure maintaining mode (the data loss rate is smaller than or equal to 0.05%). The device and the method solve the problems of single simulation and low precision in the prior art, the correlation between the detection result and the real marine environment is improved by 85%, and a reliable platform is provided for the corrosion resistance test of the ocean engineering cementing material.

The present application relates to sewage remediation technical field, particularly to a kind of in-situ microbial pumping and injection remediation method of acid uranium mining groundwater.The method provided by the present application has the following advantages:1) the present application uses contaminated raw water to culture sulfate-reducing bacteria into bacterial solution, and continuously injects bacterial solution into the area to be repaired, and supplements new culture medium, so as to realize the continuous pumping and injection of bacterial solution, by reasonably controlling the continuous pumping and injection ratio (15%, 20% pumping and injection alternately), increase the reproduction and expansion rate of functional microorganisms, enhance the self-reproduction and repair effect of in-situ microorganisms, realize the efficient removal of iron, uranium and sulfate, reduce the degree of water pollution;2) low cost: sulfate-reducing bacteria is a kind of biological catalyst that can self-reproduce, by suitable pumping and injection ratio, in-situ biological sustainable remediation effect can be realized, and the cost of groundwater pumping and treatment is reduced;3) environmentally friendly: sulfate-reducing bacteria is non-toxic and harmless to the environment, and will not cause secondary pollution.

The invention relates to a method for producing acid through co-fermentation of sulfur reduction reinforced waste oil and excess sludge. Comprising the following steps: 1) treating residual sludge, standing for a period of time, and removing part of supernate to obtain treated residual sludge; (2) putting the residual sludge treated in the step (1) into a reaction device, and then adding waste oil; (3) adding sulfide into the reaction device; and 4) fermenting in an anaerobic environment. According to the present invention, the sulfur substance is added as the electron acceptor, such that the LCFA degradation can be effectively promoted, the sulfur substance can effectively promote the acid production under the alkaline condition, and the incompletely oxidized sulfate reducing bacteria (SRB) is successfully enriched so as to alleviate the LCFA inhibition effect and significantly improve the acid production efficiency;

The invention relates to the field of oil-gas field development, and discloses a method for removing sulfur-containing gas in an oil well by a biological method, which comprises the following steps: injecting a medicament A with the concentration of y1mg / L into the oil well according to a correlation formula y1 = 20a * Lgx1, x1 > 2; injecting an agent A with the concentration of y1mg / L into the oil well according to a correlation formula y1 = 20a * Lg2, wherein x1 is smaller than or equal to 2; injecting an agent B with the concentration of y2mg / L into the oil well according to a correlation formula of y2 = 30b / Lgx2, wherein x2 is greater than 2; injecting an agent B with the concentration of y2mg / L into the oil well according to a correlation formula of y2 = 30b / Lg2, wherein x2 is less than or equal to 2; wherein the concentration of the sulfate reducing bacteria in the oil well is x1CFU / mL, the concentration of the beneficial bacteria in the oil well is x2CFU / mL, a and b are actual value coefficients, a is smaller than or equal to 2, and b is larger than or equal to 1 and smaller than 10; the agent A comprises a sulfur removal bacterial agent; the medicament B comprises a nutritional agent. The reagent concentration is determined by utilizing a correlation formula, the reagent adding stability is realized, the desulfurization effect can be effectively improved, the reagent adding is more scientific, and the desulfurization cost is reduced.

The application provides a wastewater treatment method based on interspecies electron transfer, and belongs to the technical field of wastewater treatment. The wastewater treatment method based on interspecies electron transfer comprises the following steps: introducing wastewater to be treated into a wastewater treatment device, wherein the wastewater treatment device comprises ammonia-oxidizing bacteria, carbon-fixing bacteria and sulfate-reducing bacteria; under anaerobic conditions, in the wastewater treatment device, the ammonia-oxidizing bacteria oxidize ammonia nitrogen in the wastewater to be treated as an electron donor to generate an oxidation reaction, the carbon-fixing bacteria reduce inorganic carbon in the wastewater to be treated as an electron acceptor to generate a reduction reaction, and the sulfate-reducing bacteria are used to combine the ammonia-oxidizing bacteria and the carbon-fixing bacteria by using interspecies electron transfer capacity of the sulfate-reducing bacteria, so that the wastewater to be treated is subjected to denitrification through metabolic interaction among multiple strains, and treated wastewater is obtained.

The invention relates to a cross-feeding complex microbial inoculant capable of synchronously removing beneficiation reagents and heavy metal pollution and application thereof, and belongs to an environmental microbial remediation and wastewater treatment technology, and the cross-feeding complex microbial inoculant is obtained by inoculating a mixed bacterial solution of a sulfur oxidizing bacteria (Thiobacillus.sp) bacterial suspension and a sulfate reducing bacteria (Desulfomacululprofrudii) bacterial suspension into a liquid culture medium, and carrying out shaking culture at 120 rpm under the conditions of constant temperature of 30 DEG C and micro-aerobic condition until the OD600 value reaches 0.6-1.0. According to the cross feeding complex microbial inoculant, through the metabolic synergistic effect of sulfur oxidizing bacteria (Thiobacillus.sp) and sulfate reducing bacteria (Desulfotomacullum profrudii), synchronous efficient degradation of xanthate and synchronous fixation treatment of heavy metals (such as Cu, Pb and Zn) are achieved in a reactor.

The invention provides a coal gangue rapid soil formation method and application thereof in ditch filling and land reclamation, and belongs to the technical field of coal gangue ecological utilization. The coal gangue rapid soil formation method comprises the following steps that a foundation layer, a parent material-like layer, a soil-like layer and an ecological surface layer are sequentially constructed in a gully from bottom to top, and ecological stockpiling is carried out; the base layer is formed by a flexible biological impermeable material and a compound bacteria solution A; the parent material-like layer is formed by low-sulfur coal gangue, an improved matrix A and a bacillus pasteurii liquid; the soil-like layer is formed by high-sulfur coal gangue, an improved matrix B and a compound bacteria solution A; the ecological surface layer is formed by an ecological base material, a compound bacteria solution B and plant seeds; the compound bacteria liquid A in the base layer and the soil-like layer comprises acidophilic sulfate reducing bacteria and bacillus pasteurii, and the compound bacteria liquid B comprises bacillus subtilis, rhizobium and nitrogen-fixing bacteria. By adopting the method disclosed by the invention, harmless and large-scale digestion treatment of the coal gangue can be realized.

The invention belongs to the technical field of environmental materials, and particularly relates to a method for electrodeposition-assisted biosynthesis of a silver-based catalytic material SSSM-SRB and application of the silver-based catalytic material SSSM-SRB. The invention relates to a method for electrodeposition-assisted biosynthesis of a silver-based catalytic material SSSM-SRB, which comprises the following steps: by taking a sulfate reducing bacteria (SRB) suspension as an electrolyte and a silver net as a working electrode, carrying out electrodeposition by adopting a cyclic voltammetry, and growing Ag2S on the surface of the silver net in situ. The SRB is used for biologically synthesizing Ag2S, the process is environmentally friendly, and use of toxic reagents in traditional chemical synthesis is avoided.

The invention relates to the technical field of anhydrous treatment, in particular to a heavy metal adsorption filler as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, mixing biomass, attapulgite, a pore-forming agent and water, and performing compression molding to obtain raw material balls; the raw material balls are sequentially subjected to first drying and anoxic pyrolysis, and filler balls are obtained; and soaking the filler balls in a sulfate reducing bacteria solution, and sequentially carrying out biofilm formation and secondary drying to obtain the heavy metal adsorption filler. The heavy metal adsorption filler obtained by the method can effectively adsorb heavy metals, has higher mechanical strength and is suitable for repairing underground water.

The present application relates to wastewater biological treatment technical field, disclose a kind of high sulphate high nitrate nitrogen wastewater denitrification processing method, comprising the following steps: (1) in anoxic zone I, anoxic zone II and aerobic zone are filled into biological membrane carrier, and the biological membrane carrier of anoxic zone I and anoxic zone II is attached with salt-tolerant anoxic denitrifying bacteria, and the biological membrane carrier of aerobic zone is attached with salt-tolerant aerobic denitrifying bacteria;(2) the carbon-nitrogen ratio of wastewater to be treated is adjusted to 1.25~1.5, then into anoxic zone I, and carries out first-stage anoxic biological treatment;(3) the effluent of anoxic zone I is introduced into anoxic zone II, and carries out secondary anoxic biological treatment;(4) the effluent of anoxic zone II is introduced into aerobic zone, and carries out aerobic biological treatment.The method of the present application can better utilize denitrifying bacteria to inhibit the enrichment of sulfate-reducing bacteria, avoid the production of toxic sulfide and hydrogen sulfide, thereby improving the efficiency of wastewater denitrification.

The invention belongs to the technical field of oil field production, oil field chemistry and bacterium detection, and particularly relates to a ball coating bacterium detection method and device and application. The ball coating bacteria detection method provided by the invention comprises the following steps: (1) pouring a sterilized culture medium into a sterile culture dish, and adding sterile balls after the culture medium is solidified to obtain a bacteria detection device; (2) adding a certain amount of bacteria-containing water to the culture medium, and shaking the balls to uniformly coat the culture medium with the bacteria-containing water; (3) inverting the bacterium detection device, and separating the ball from the culture medium; (4) culturing the bacteria detection device to obtain a bacterial colony; and (5) identifying and counting bacterial colonies, and calculating to obtain the number and concentration of bacteria in the bacteria-containing water body. The saprophytic bacteria, the iron bacteria and the sulfate reducing bacteria can be qualitatively and quantitatively detected, the detection result is accurate and reliable, and the repeatability is high.

The application provides a sulfur-mediated anaerobic-anoxic-aerobic coking wastewater treatment method and system. The method is characterized by inoculating specific bacteria in an anaerobic reactor, an anoxic reactor and an aerobic reactor which are sequentially connected, and then making the coking wastewater to be treated sequentially pass through the anaerobic reactor, the anoxic reactor and the aerobic reactor, and then precipitating in a sedimentation tank, so as to realize wastewater treatment. In the above process, the coking wastewater to be treated sequentially passes through the anaerobic reactor inoculated with sulfate-reducing bacteria, the anoxic reactor inoculated with sulfur autotrophic denitrifying bacteria and the aerobic reactor inoculated with nitrifying bacteria, and the "sulfur cycle" is embedded in the coking wastewater treatment process, so that various pollutants in the coking wastewater can be effectively removed.

This invention relates to a sulfur autotrophic denitrification coupled with anaerobic ammonia oxidation filter and its operation method based on a backwashing strategy to achieve hydrogen sulfide reduction and sulfur source regeneration, belonging to the field of biological wastewater treatment. A backwashing device is installed at the bottom of the filter, and sulfur-containing filter media is dispersed within the filter. The filter outlet is equipped with a dissolved oxygen coupled with a temperature probe and a hydrogen sulfide probe, connected to a real-time monitoring and control system. The sulfur-containing filter media serves as a carrier for the attachment and growth of sulfur autotrophic denitrifying bacteria, sulfate-reducing bacteria, and anaerobic ammonia oxidizing bacteria. The real-time monitoring and control system is configured to activate the backwashing device when the hydrogen sulfide concentration exceeds a set value, injecting a set amount of gas into the filter for backwashing. While ensuring the smooth progress of sulfur autotrophic denitrification, anaerobic ammonia oxidation is coupled, and the oxygen from the backwashing is used to oxidize the hydrogen sulfide, converting it into elemental sulfur, which continues to serve as a sulfur source to drive sulfur autotrophic denitrification and achieve sulfur source regeneration. This effectively reduces the hydrogen sulfide content.

The invention relates to the technical field of sulfate reducing bacteria, in particular to a preparation method of a space-functionalized sulfate reducing bacteria blanket, which comprises the following steps: firstly, preparing a three-layer composite carrier consisting of a carbon felt bottom layer, a nitrogen-doped graphene / Fe3O4 conductive middle layer and a slow-release nutrition top layer; then, utilizing the synergistic effect of an electric field with specific intensity and an alternating / constant magnetic field to directionally colonize an acid-producing and hydrogen-producing flora and an acetic acid oxidation type and hydrogen nutrition type sulfate reducing flora in different space depths of the carrier in sequence to form a vertical metabolism partition; and finally, maturing the fungus blanket through multi-stage gradient domestication containing a fluctuating carbon source and weak current stimulation. According to the improvement, the problems of low treatment efficiency, poor impact resistance and long-term operation performance degradation caused by disordered spatial distribution of functional microorganisms, easy breakage of metabolic chains, unstable flora structure and lack of in-situ sensing regulation capability when the traditional sulfate reducing flora is applied to high-volatility rare earth tail water treatment are solved.

The invention discloses a multi-dimensional quantitative detection method for microbial corrosion resistance of Cr-containing steel. The method comprises the following steps: a microbial challenge test: inoculating sulfate reducing bacteria into a culture medium, and exposing a sample in a microbial environment; performing quantitative analysis on corrosion products: performing ultrasonic rust removal on the sample by using an acid pickling solution, then calculating a weightlessness corrosion rate, and quantifying the adhesion amount of microorganisms on the surface of the sample; multi-mode electrochemical testing: monitoring the open-circuit potential and linear polarization resistance of the sample, and performing electrochemical impedance spectroscopy and potentiodynamic polarization curve testing to obtain corrosion current density and polarization resistance; surface analysis: analyzing the corrosion morphology and element distribution of the sample through a scanning electron microscope and an energy disperse spectroscopy, and determining the chemical state of the chromium element by adopting X-ray photoelectron spectroscopy to obtain the stability and the sulfur content of a passive film; and evaluating the corrosion degree: establishing a coupling model of the corrosion current density, the polarization resistance, the passivation film stability, the microorganism adhesion amount and the sulfur content, and calculating the corrosion degree.

This invention discloses a microbial corrosion method for preparing Al for hydrogen production by water electrolysis. 0.6 The method for developing a CrFe2Ni2 high-entropy alloy catalyst belongs to the field of electrochemical catalysis technology. This invention uses sulfate-reducing bacteria (SRB) as the experimental strain and bulk Al as the catalyst. 0.6 A CrFe2Ni2 high-entropy alloy was used as the matrix material. After SRB corrosion, the FeS corrosion product generated on the alloy surface promoted the catalytic activity of the material. The resulting catalyst was used as the working electrode for water electrolysis to produce hydrogen, exhibiting excellent OER activity and durability in alkaline solutions. Compared with existing technologies, this invention is the first to combine microbial corrosion with bulk alloys for industrial hydrogen production via water electrolysis. The preparation method is simple and mild, with potential application prospects, and provides theoretical guidance and technical support for the design and synthesis of other non-precious metal water electrolysis catalysts.