19 results about "Enzyme Interaction" patented technology

In various embodiments a molecular circuit is disclosed. The circuit comprises a negative electrode, a positive electrode spaced apart from the negative electrode, and an enzyme molecule conductively attached to both the positive and negative electrodes to form a circuit having a conduction pathway through the enzyme. In various examples, the enzyme is a polymerase. The circuit may further comprise molecular arms used to wire the enzyme to the electrodes. In various embodiments, the circuit functions as a sensor, wherein electrical signals, such as changes to voltage, current, impedance, conductance, or resistance in the circuit, are measured as substrates interact with the enzyme.

The application relates to the field of biotechnology, and discloses a method and system for regulating the ratio of amino acids and peptides after soybean proteolysis, which comprises collecting enzyme-coupling parameters in the whole proteolysis process to generate time series data, obtaining a multi-dimensional dynamic covariance matrix based on the data, identifying significant enzyme interaction through the matrix, and generating a list; a multi-level enzyme interaction network model is constructed according to the list, and finally, dynamic and accurate regulation of the ratio of amino acids and peptides is realized based on the model; the application can effectively solve the identification problem of hidden interaction interference in a multi-enzyme system, improve the consistency and predictability of product functions, and reduce the batch-to-batch fluctuation of the content of target functional peptides.

A method for selecting polynucleotides, the method comprising: allowing a nucleic acid handling enzyme to move along multiple polynucleotides in a sample for a defined time period, wherein the enzyme is loaded onto each of the multiple polynucleotides and wherein one or more molecule of the enzyme moves along each of the multiple polynucleotides; and selecting polynucleotides based on whether or not the enzyme reaches the end of and / or unbinds from the polynucleotides in the defined time period.

In various embodiments a molecular circuit is disclosed. The circuit comprises a negative electrode, a positive electrode spaced apart from the negative electrode, and an enzyme molecule conductively attached to both the positive and negative electrodes to form a circuit having a conduction pathway through the enzyme. In various examples, the enzyme is a polymerase. The circuit may further comprise molecular arms used to wire the enzyme to the electrodes. In various embodiments, the circuit functions as a sensor, wherein electrical signals, such as changes to voltage, current, impedance, conductance, or resistance in the circuit, are measured as substrates interact with the enzyme.

The present disclosure provides methadone prodrugs, pharmaceutical compositions, and their methods of use, where the pharmaceutical compositions comprise a methadone prodrug that provides enzymatically-controlled release of methadone, and an optional enzyme inhibitor that interacts with the enzyme(s) that mediates the enzymatically-controlled release of methadone from the prodrug so as to attenuate enzymatic cleavage of the prodrug.

The present disclosure relates to enzymatic cascade reactions for achieving better sensitivity in detecting a target nucleic acid sequence. Several aspects of the disclosure relate to a reaction master mix comprising four major components: a CRISPR enzyme, a guide RNA (gRNA), an aptamer (e.g., an inhibiting aptamer or an activating aptamer) and a “signaling” (i.e., reporter) enzyme. The aptamer interacts with the signaling enzyme and forms a complex, resulting in, e.g., inhibited signaling enzyme activity. When a target is present in the reaction mix, the Cas / guideRNA system becomes activated and preferentially collaterally cleave(s) all nucleic acids in the solution, including the aptamer. Once aptamer gets cleaved, the signaling enzyme is free to produce a signal. In the presence of a substrate, such signaling enzyme activity produces a robust signal that significantly improves the limit-of-detection of other techniques in the art.

The present invention provides a regulator construct in which the drug efficacy is auto-regulated by the presence of a indicator enzyme. The construct is a chemical structure comprising: (a) a biorecognition component comprising a substrate recognized by an enzyme, (b) an incapacitation component, and (c) an active drug component. The incapacitation component provides a chemical incapacitation mechanism to render benign or incapacitate the drug component, after the substrate-enzyme interaction, allowing additionally for the creation of altered benign chemical structures while, otherwise, when said enzymes is inactive or absent the drug will take effect.

The invention relates to the technical field of biology, and discloses a method and a system for regulating and controlling the proportion of amino acid and peptide after soybean protein enzymolysis, the method comprises the following steps: collecting enzymolysis coupling parameters in the whole enzymolysis process to generate time sequence data, obtaining a multi-dimensional dynamic covariance matrix based on the data, identifying significant enzyme interaction through the matrix, and generating a list; and constructing a multilevel enzyme interaction network model according to the list, and finally realizing dynamic and accurate regulation and control of the amino acid and peptide proportion based on the model. The method can effectively solve the identification problem of recessive interaction interference in a multi-enzyme system, improve the consistency and predictability of product functions, and reduce the batch-to-batch fluctuation of the target functional peptide content.

The present disclosure provides methadone prodrugs, pharmaceutical compositions, and their methods of use, where the pharmaceutical compositions comprise a methadone prodrug that provides enzymatically-controlled release of methadone, and an optional enzyme inhibitor that interacts with the enzyme(s) that mediates the enzymatically-controlled release of methadone from the prodrug so as to attenuate enzymatic cleavage of the prodrug.

The present invention belongs to the field of molecular biology, and relates to plant growth-promoting rhizobacteria (PGPR), and in particular to a method and an engineered bacterium for improving the rhizosphere chemotaxis and colonization ability of PGPR. The present application discovered that at least one of the bacterial chemotaxis receptors has a pentapeptide in its C-terminal extension region, and the pentapeptide can mediate the interaction between the methyltransferase CheR and the methylesterase CheB involved in chemotaxis, and reversibly methylate the chemotaxis receptor. Grafting the pentapeptide onto the C-terminus of the chemotaxis receptor of a strong chemoattractant in the chemosphere of the PGPR can enhance its chemotactic response to the strong chemoattractant, thereby improving the rhizosphere chemotaxis and colonization ability of the PGPR.

The present disclosure relates to enzymatic cascade reactions for achieving better sensitivity in detecting a target nucleic acid sequence. Several aspects of the disclosure relate to a reaction master mix comprising four major components: a CRISPR enzyme, a guide RNA (gRNA), an aptamer (e.g.., an inhibiting aptamer or an activating aptamer) and a "signaling" (i.e., reporter) enzyme. The aptamer interacts with the signaling enzyme and forms a complex, resulting in, e.g., inhibited signaling enzyme activity. When a target is present in the reaction mix, the Cas / guideRNA system becomes activated and preferentially collaterally cleave(s) all nucleic acids in the solution, including the aptamer. Once aptamer gets cleaved, the signaling enzyme is free to produce a signal. In the presence of a substrate, such signaling enzyme activity produces a robust signal that significantly improves the limit-of-detection of other techniques in the art.

The skin care composition provides a skin care composition capable of interacting with enzymes in the stoma output, thereby reducing enzyme activity.SOLUTION: A skin care composition comprising at least 1 wt.% potato protein, a first binder comprising a polar or non-polar polymer or copolymer in an amount of 20 to 70 wt.%, and 20 to 80 wt.% solids.SELECTED DRAWING: None

Compounds are described herein for the detection of the activity of an enzyme. The compounds include a recognition domain / substrate structured to interact with the enzyme, a reporter molecule, and a linking group forming a covalent bond between the recognition domain and reporter molecule. Upon interaction of the recognition domain with the enzyme, the covalent bond is destroyed, rendering reporter molecule detectable by a chemical detection device. Methods of detecting enzymatic activity of a plurality of enzymes are also described herein. Such methods include reacting a compound (having a recognition domain / substrate structured to interact with the enzyme, a reporter molecule, and a linking group forming a covalent bond between the recognition domain and reporter molecule) with an enzyme, and identifying detectable reporter molecules with a chemical detection device.

The invention relates to the technical field of enzyme catalysis, and discloses a feed fermentation parameter control method and system.The feed fermentation parameter control method comprises the steps that a metabolome similarity index is calculated through metabolite distribution; constructing an inter-enzyme interaction model according to the enzyme activity incidence matrix and the enzyme activity; defining a fermentation efficiency function, and solving the fermentation efficiency function to obtain an optimal parameter combination; calculating a parameter deviation between the current actual parameter value and the optimal fermentation parameter combination, and calculating a fermentation parameter quantity needing to be adjusted according to the parameter deviation; through inter-enzyme interaction network construction and comprehensive parameter control, the overall efficiency of the fermentation process is improved, the fermentation period is shortened, and the yield is improved.

This invention relates to a method for preparing oyster sauce, particularly a method based on the synergistic preparation of oyster sauce using bacterial-enzyme interaction and gradient temperature-variable thermal reaction. The method includes raw material pretreatment, bacterial-enzyme interaction hydrolysis, and gradient temperature-variable thermal reaction. This invention employs a process route combining bacterial-enzyme interaction with complex hydrolysis and a step-by-step gradient temperature-variable thermal reaction. During bacterial fermentation, endonucleases are responsible for efficiently cleaving peptide bonds within proteins, generating a large number of small peptides and amino acids, providing ample precursor substances for the gradient temperature-variable thermal reaction. These precursors are more readily combined with reducing sugars during the reaction. Exonucleases, on the other hand, hydrolyze hydrophobic terminal peptide bonds, reducing the production of bitter peptides and improving the product flavor. The subsequent gradient temperature-variable thermal reaction generates basic aroma substances at low temperatures, followed by a deeper reaction at high temperatures, forming complex and rich aroma compounds with flavors such as roasted meat and nuts. These two processes complement each other, jointly constructing the rich flavor and aroma profile of the oyster sauce.

The present application relates to genetic engineering body, specifically discloses a schizonepeta MYB transcription factor gene SMYB71 And virus-induced gene silencing vector and application, the nucleotide sequence of the gene is shown as SEQ ID NO.1, the amino acid sequence of the coding product is shown as SEQ ID NO.2, the cloning, amplification, inhibition in schizonepeta in vivo of schizonepeta MYB transcription factor gene St MYB71 silencing fragment, schizonepeta MYB transcription factor StMYB71 inhibited schizonepeta plant is obtained, and the yeast single hybridization technology is further used to determine that StMYB71 regulates schizonepeta monoterpene compound biosynthesis by interacting with schizonepeta limonene-3-hydroxylase StL3OH.The present application relates to genetic engineering body, specifically discloses a schizonepeta MYB transcription factor gene St MYB71, the problem that the related MYB transcription factor for regulating monoterpene synthesis by interacting with StL3OH has not been separated and identified in the prior art research on schizonepeta can be solved.The present application can provide a basis for gene engineering for improving schizonepeta and improving the content of effective components such as schizonepeta pulegone and limonene.

A sensor and method for measuring enzymatic activity, such as cholinesterase (CHE) enzymatic activity. The sensor comprises a near-infrared (NIR) fluorescent single-walled carbon nanotube (SWCNT) functionalized with a substrate such as myristoylcholine (MC). Quantitative analysis correlates changes in fluorescence of the SWCNTs with enzymatic activity. A decrease in NIR fluorescence intensity of SWCNTs upon interaction of the substrate with the target enzyme allows real-time monitoring and is positively correlated with enzymatic activity. An absence of modulation of fluorescence of the SWCNTs is indicative of enzymatic inhibition and the presence of enzyme inhibitors.

The invention belongs to the technical field of biological medicine, and particularly relates to a biological switchable siRNA delivery composite material based on RNAi and a preparation method and application thereof. The invention designs a novel biological switchable siRNA delivery system (BiRDS) based on RNAi (Ribonucleic Acid Interference) by combining good endocytosis performance and stability of tetrahedral framework nucleic acid and gene targeting specificity of siRNA, the system consists of three siRNAs (three magic birds) and a nucleic acid core (central sun), and by loading small interfering RNA to target casein kinase 2 interacting protein 1 (siCkip-1), the novel biological switchable siRNA delivery system (BiRDS) based on RNAi is obtained. The siRNA is specifically delivered to the bone formation surface, the loading capacity is improved to the maximum extent, the kip gene is efficiently silenced, cell proliferation and migration are promoted, the good osteogenesis induction effect is achieved, and a new thought is provided for treatment of maxillofacial bone defects.

Compositions are described herein for the detection of the activity of a plurality of enzymes. The compositions comprise a plurality of molecular probes. Each molecular probe comprises a recognition domain / substrate structured to interact with a specific enzyme, a reporter molecule, and a linking group forming a covalent bond between the recognition domain and reporter molecule. For each of the plurality of molecular probes, upon interaction of the recognition domain with specific enzyme, the covalent bond is destroyed, rendering reporter molecule detectable by a chemical detection device. Methods of detecting enzymatic activity of a plurality of enzymes are also described herein. Such methods comprise reacting a composition, as described herein, with a plurality of enzymes, and identifying detectable reporter molecules with a chemical detection device.