This invention relates to a method for preparing
oyster sauce, particularly a method based on the synergistic preparation of
oyster sauce using bacterial-
enzyme interaction and gradient temperature-variable
thermal reaction. The method includes
raw material pretreatment, bacterial-
enzyme interaction
hydrolysis, and gradient temperature-variable
thermal reaction. This invention employs a process
route combining bacterial-
enzyme interaction with complex
hydrolysis and a step-by-step gradient temperature-variable
thermal reaction. During bacterial
fermentation, endonucleases are responsible for efficiently cleaving
peptide bonds within proteins, generating a large number of small peptides and amino acids, providing ample precursor substances for the gradient temperature-variable thermal reaction. These precursors are more readily combined with reducing sugars during the reaction. Exonucleases, on the other hand, hydrolyze hydrophobic terminal
peptide bonds, reducing the production of bitter peptides and improving the product
flavor. The subsequent gradient temperature-variable thermal reaction generates basic
aroma substances at low temperatures, followed by a deeper reaction at high temperatures, forming complex and rich
aroma compounds with flavors such as roasted meat and nuts. These two processes complement each other, jointly constructing the rich
flavor and
aroma profile of the
oyster sauce.