84 results about "Genipinine" patented technology

The present invention relates to novel genipin derivatives which have an excellent liver protection activity with little cytotoxicity, and these compounds are so stable in vivo that they do not induce any side effects.

The invention discloses genipin cross-linked biogel as well as a preparation method and application thereof. The genipin cross-linked biogel comprises the following raw materials in percentage by weight: 0.05wt%-0.2wt% of genipin, 1wt%-10wt% of matrix and the balance of water. By carrying out crosslinking by virtue of optimized genipin concentration, the obtained biogel has the properties of low toxicity, good water-absorbing property and mechanical property and the like. The biogel can be directly used for protecting and isolating the surfaces of various wounds, bedsores and ulcers, can be used as a dressing carrier to load antibacterial drugs, paregorics, growth factors and the like, and can be applied to the treatment of wound infection, wound analgesia or promotion of wound healing and the like. According to the genipin cross-linked biogel, the matrix, a crosslinking agent and the components are all derived from natural organisms, so that the biogel is safe and non-toxic and can be widely applied to the treatment and repair of clinical acute and chronic wounds.

Novel compositions comprising genipin for cross-linking polymer fibers, are provided. In aspects of the invention the compositions further comprise a solvent system, wherein said solvent system comprises alcohol solvent and water. The genipin-based compositions are useful in methods for promoting the stabilization of fibers in an aqueous environment, and in tissue engineering. The novel genipin-based composition is also useful in methods of treating dermatological conditions.

The invention belongs to the field of medicines and particularly relates to a new application of an iridoid glycoside compound, genipin-1-[beta]-D-gentiobioside, and a composition composed by compounding the genipin-1-[beta]-Dgentiobioside with other components as effective components for preparing antiviral drugs, antibacterial drugs, fever alleviating drugs, inflammation resistant drugs and anti-oxidizing drugs. The iridoid glycoside compound and the composition can be used for treating acute respiratory infection, flu, pneumonia, viral hepatitis type B, herpes zoster, herpes viral keratitis, viral myocarditis, Epstein-Barr virus infection, retrovirus infectious diseases and bacterial infectious diseases.

The invention discloses a method for producing a hemostatic composition comprising mixing a biocompatible polymer suitable for use in hemostasis and a genipin-type crosslinker, crosslinking said polymer by said genipin-type crosslinker to obtain a crosslinked biocompatible polymer, and finishing said crosslinks polymer to a pharmaceutically acceptable hemostatic composition, new hemostatic compositions and methods for using such compositions.

The invention relates to a glycosyl substituted genipin derivative (I) as well as a preparation method and an application as pesticides. The inhibition rate of a part of compounds in formulae (Ia andIb) to tobacco mosaic viruses exceeds that of commercial variety virazole, and is approximate to that of ningnanmycin. In addition, a part of the compounds in the formulae (Ia and Ib) have excellent sterilization activity on 14 plant pathogens, and fungus bacteriosis of multiple crops can be effectively prevented and treated. A part of the compounds in the formulae (Ia and Ib) have excellent insecticide activity on 4 common lepidoptera pests (plutella xylostella, oriental armyworm, ostrinia nubilalis and cotton bollworm), and lepidoptera pests of multiple crops can be effectively prevented andtreated. Specific synthesis steps and bioactivity are shown in the specification.

The invention discloses a geniposide-rich hydrophilic molecularly imprinted polymer, a preparation method and application, and belongs to the technical field of separation. Template geniposide, an alkenyl glucoside functional monomer, a cross-linking agent, an initiator and an pore-forming agent are added in an aqueous solvent system, the alkenyl glucoside functional monomer and the template molecule form a non-covalent template-monomer complex through invertible self-assembly, the template geniposide is removed, and the geniposide-rich hydrophilic molecularly imprinted polymer is obtained. The alkenyl glucoside functional monomer is used for the first time to prepare the geniposide molecularly imprinted polymer in the water phase; compared with a traditional molecularly imprinted polymer prepared from a liposoluble functional monomer, the polymer prepared in the water phase has good hydrophilia, and has better adsorption performance in a water medium.

The invention discloses an application of geniposide which is used as an acetylcholin esterase inhibitor in a medicament for treating and preventing diseases with acetylcholin esterase activity abnormity. The invention relates to geniposide which directly inhibits activity of the acetylcholin esterase, inhibits activity of acetylcholin esterase in a cultured nerve cell, and inhibits activity of the acetylcholin esterase in a transgenic animal brain with Alzheimer's disease. The geniposide can bring inhibition effect on the activity of the acetylcholin esterase into play by virtue of the way, but is not limited to the way, and the geniposide possibly has a potential treatment value on the Alzheimer's disease by inhibiting the acetylcholin esterase.

The invention discloses a beta-glucosidase high-yielding strain. The beta-glucosidase high-yielding strain is screened from the nature, with fructus gardenia as a carbon source, and has a preservation number of CCTCC No.M2013634 and is classified and named as penicillium rolfsii HXL; the strain is able to efficiently convert geniposide and polydatin into genipin and resveratrol; on that basis, a method for converting and preparing genipin and resveratrol by using the beta-glucosidase high-yielding strain above is created. The method has the advantages that geniposide and polydatin are respectively hydrolyzed under a mild condition, so as to avoid the shortage of the loss of active ingredients of the traditional method, and thus the yield is effectively increased; in addition, the active ingredients, namely, geniposide or polydatin, of fructus gardenia or polygonum cuspidatum are converted by mass under an in-vitro controllable condition by the modern microbial fermentation engineering technology and enzyme engineering technology, and thus the production cost is decreased, and the production efficiency is improved. With the adoption of the beta-glucosidase high-yielding strain, a feasible manner is provided for industrially producing beta-glucosidase, genipin and resveratrol as mass, large economic benefit and social benefit are brought, and the market development prospect is wide.

The invention discloses a method for extracting aucubin and geniposide from eucommia ulmoides peel at the same time. The method includes the steps of firstly, weighing aucubin, geniposide and cobaltous acetate to be dissolved in an acetonitrile-dimethyl sulfoxide mixed solution according to a certain proportion, conducting shaking, adding functional monomers to be shaken, adding mixed cross-linking agent TRIM-DVB and initiator AIBN to be sufficiently mixed, transferring the mixture into a round-bottom flask, and conducting degassing, argon introducing and bottle sealing; secondly, putting the mixture in a constant-temperature water bath kettle at the temperature of 60 DEG C to be subjected to a polymerization reaction, and conducting smashing, screening, depositing, filtering, eluting and vacuum drying; thirdly, putting obtained molecularly imprinted polymers into a solid-phase extraction cylinder, conducting washing and eluting after sample adding, and obtaining aucubin and geniposide. Through the metal ion coordination imprinting technology, binary compound metal ion complex serves as a template molecule, molecule imprinting polymers with high selection recognition performance is synthesized, and quite high practical value is achieved when aucubin and geniposide are extracted from eucommia ulmoides peel at the same time through the polymers.

The invention discloses a preparation method of an immobilized enzyme. The preparation method comprises the following steps: performing cross-linking on polysaccharide sodium alginate with genipin; adding beta-glucosidase, and polymerizing to obtain a polymerized solution; dropwise adding the polymerized solution into a CaCl2 solution to form immobilized beads; performing metal ion substitution on the immobilized beads in a MnSO4 solution to form hybrid manganese alginate immobilized particles. The invention further discloses a geniposide conversion method which comprises the following steps: performing continuous bioconversion on the prepared hybrid manganese alginate immobilized particles in a packed bed reactor (PBR), and hydrolyzing geniposide to prepare the genipin. The preparation method has the benefits that through an immobilization method, the immobilized enzyme is better in stability and hydrolysis performance in comparison with a free enzyme, the time for achieving the same hydrolysis effect is shortened by more than half, and the hydrolysis process is more complete; the duration time is more than 500 hours and the conversion rate is kept about 90% during the continuous conversion process in the PBR.

The present invention discloses one kind of total jasminoidin extract and features that the total jasminoidin extract contains jasminoidin 45-90 wt%, genipin-1-beta-D-gentiobioside 5-30 wt% and jasminoidin + genipin-1-beta-D-gentiobioside 50-100 wt% and preferably contains jasminoidin 50-80 wt%, genipin-1-beta-D-gentiobioside 5-25 wt% and jasminoidin + genipin-1-beta-D-gentiobioside 55-100 wt%. Pharmacological experiment shows that the total jasminoidin extract of the present invention has excellent pharmacological effect.

The invention discloses a preparation method of eucommia ulmoides geniposidic acid composite nanoparticles, zein loaded geniposidic acid composite nanoparticles are prepared by using the unique self-assembly characteristic of zein, taking polysaccharide as a stabilizer and utilizing an anti-solvent coprecipitation method; the operation is simple, the condition is mild, the cost is lower, and the method is green and environment-friendly. According to the zein-loaded geniposidic acid composite nano-particles, geniposidic acid is embedded in the protein nano-particles, so that the stability of the protein nano-particles is effectively improved, the solubility of the protein nano-particles is improved, the prepared zein-loaded geniposidic acid composite nano-particles are uniform in particle size, high in embedding rate and relatively good in stability, the bioavailability of the natural active ingredient geniposidic acid is improved, and the bioavailability of the zein-loaded geniposidic acid composite nano-particles is improved. The problems that geniposidic acid is unstable and difficult to dissolve are solved.

A temporary tattooing ink is produced from concentrated genipin. In one embodiment, the concentrated genipin forms part of a solution. In another embodiment, the genipin is provided in a gel form which also includes a solvent and a thickening agent. Finally, an applicator is described into which genipin may be embedded for applying to a user's skin.

The invention discloses application of a genipin amino acid derivative as an NF-kappa B (nuclear transcription factor kappa-B) inhibitor. The genipin amino acid derivative as the NF-kappa B inhibitor controls the expression of various inflammatory cytokines, major histocompatibility composite gene and adhesion molecules, which participate in the tumor metastasis, and restrains and treats pathological symptoms caused by NF-kappa B activation by blocking the nuclear factor NF-kappa B activation, wherein the symptoms include cancer, autoimmune diseases (such as rheumatoid arthritis), cardiovascnlar and cerebrovascular diseases, acute and chronic inflammations, nervous system disorders (depression, Alzheimer's disease, etc.). The genipin amino acid derivative can restrain the NF-kappa B activity in a dose and time-dependent manner. The invention provides a novel drug source for NF-kappa B inhibitors and NF-kappa B control related diseases.

The invention discloses an application of geniposide in preparation of medicines for treating and preventing a radioactive lung injury caused by ionizing radiations. The geniposide as the medicines for treating and preventing the radioactive lung injury caused by aerospace, nuclear radiation and thoracic cancer radiotherapy strengthens the immunity of the organism to alleviate the radiation injury, so that the geniposide can provide a selective radiation protection idea for the radioactive lung injury caused by ionizing radiations, including an aerospace business, a nucleus scientific and technological development and a breast cancer radiotherapy and the like.

The invention discloses application of a beautifying cornea lens, and aims at applying the beautifying cornea lens to repairing the damage of cornea tissues, improving the appearance of an ocular surface, curing visual symptoms such as photophoby, glaring, diplopia and the like resulting from iris abnormity, or being used as a covering sheet or a medicine carrier for protecting the cornea in an ophthalmic operation, wherein the beautifying cornea lens is prepared in a way that: a biological material rich in amino is added into a solution containing genipin or genipin derivatives, the genipin is taken as a cross-linking agent to conduct the contact reaction, and then the mixture is rinsed, frozen and dried to prepare the beautifying cornea lens. The beautifying cornea lens provided by the invention can be used for repairing the damage of the cornea tissues, improving the appearance of the ocular surface, curing the visual symptoms such as photophoby, glaring, diplopia and the like resulting from iris abnormity, or being used as the covering sheet or the medicine carrier for protecting the cornea in the ophthalmic operation, therefore, a new approach is directly explored for clinical application.

The invention belongs to the field of compound synthesis, and particularly relates to a geniposide derivative with xanthine oxidase inhibitory activity as well as a preparation method and application of the geniposide derivative. The geniposide derivative has a molecular structure shown in the specification. According to the invention, a novel geniposide derivative is synthesized, and the geniposide derivative has an obvious effect of inhibiting the activity of xanthine oxidase; the geniposide is subjected to structural modification, so that the biological activity of the geniposide can be greatly improved, and the geniposide has important practical significance on research and development of novel uric acid reducing drugs; according to the geniposide derivative with the xanthine oxidase inhibitory activity, related structural modification is carried out by taking geniposide as a raw material, the synthesis method is simple, the raw material price is low, and the structure is novel.

The invention discloses an artificial skin graft for genipin immobilized activin B and a preparation method thereof. The artificial skin graft comprises hydrogel prepared from a skin graft stent material, and activin B which is distributed in the hydrogel and is cross-linked through genipin. The preparation method comprises the following steps of: sequentially adding an aqueous solution of genipin and the activin B into an aqueous solution of the skin graft stent material, uniformly mixing, forming, processing, gelatinizing and obtaining the product. The product is non-toxic, does not have pyrogenicity and has a high biocompatibility; pathogenic microorganism intrusion can be prevented, and infection incidence is reduced; quick healing of epithelial tissues can be promoted; and moreover, the exudation of wound surface fluid is reduced, a locally wet microenvironment is kept, the conventional disinfection and sterilization can be performed, and the artificial skin graft is convenient to storage and transport and is applied to burn, trauma and other skin injury repair.

The invention belongs to the technical field of traditional Chinese medicine preparation, and particularly relates to a composition containing pinoresinol diglucoside and genipin and application thereof. The composition containing pinoresinol diglucoside and genipin is prepared from the following components: pinoresinol diglucoside, genipin, nicotinamide, quercetin, a radix achyranthis bidentatae extract, tartronic acid and citric acid. The composition is simple in preparation method, can be used for treating hypertensive nephropathy, has a good curative effect, and can reduce side effects and reduce cost.

The invention relates to a preparation method and application of a novel modified egg white cell culture scaffold material. The method includes: step one, adding genipin into sterile ultrapure water, stirring, oscillating ultrasonically and completely, thoroughly dissolving at 25-40 DEG C, adding 0.2ml of the genipin solution into a reaction vessel containing 2ml of fresh egg white, and well mixing; step two, putting the egg white with the genipin serving as a crosslinking agent into a thermostat to realize complete crosslinking; step three, using a pipette for dripping a modified egg white cell culture scaffold material obtained at the step two into a PBS solution drop by drop to guarantee immersion of materials without overflowing, standing for more than 12 hours to remove surplus genipin molecules, and repeating for several times; step four, putting a modified egg white cell culture scaffold material obtained after reaction on a cell clean bench, and performing ultraviolet radiation for sterilization to obtain the novel modified egg white cell culture scaffold material. The hardness gradient of the novel modified egg white cell culture scaffold material is easy to control, and safety and avoidance of toxic and side effects are realized.

The invention discloses a medical application of an iridoid glycoside compound, and particularly relates to an application of genipin 1-beta-D gentiobioside. The genipin 1-beta-D gentiobioside has the advantages that the expression of triglyceride TG and inflammation related genes TNF-alpha and IL-6 on in-vitro cells is obviously reduced; the content of animal serum ALT and liver TG on a non-alcoholic fatty liver model mouse induced by high fat and high glucose is reduced; and the expression of NLRP3 related genes can be inhibited in vivo and in vitro. Therefore, the genipin 1-beta-D gentiobioside can be used for preparing the medicine and other products for preventing and treating the non-alcoholic fatty liver disease.

The invention belongs to the technical field of hydrogel material preparation, and particularly relates to a preparation method of injectable high-pressure-resistant high-strength anti-freezing genipin cross-linked gelatin hydrogel. The method comprises the steps: firstly, dissolving gelatin to obtain a gelatin aqueous solution; dissolving genipin by using ethanol, dropwise adding a gelatin solution while uniformly stirring and mixing, standing to obtain genipin water gel, and soaking in a glycerol solution to obtain glycerol-genipin gelatin hydrogel. The genipin cross-linked gelatin hydrogel prepared by the method has high strength, freezing resistance and long-term stability, and has excellent fatigue resistance and pressure resistance. The hydrogel added with glycerol can maintain the original properties after long-term storage, can maintain high flexibility at low temperature, and has excellent frost resistance and long-term stability. Meanwhile, the mechanical property of the genipin hydrogel can be regulated and controlled according to different crosslinking degrees, and the prepared hydrogel has injectability and is expected to be widely applied to the fields of biomedical materials and the like.

The invention provides application of geniposide in preparation of a medicine for treating asthenospermia. Experimental results show that the geniposide can significantly improve the sperm quantity and activity and reduce the sperm malformation rate, can significantly inhibit endoplasmic reticulum stress inside testis, increase mitochondrial membrane potential, regulate expression of related proteins in testis tissues, and improve excessive apoptosis of spermatogenic cells.

The present disclosure provides colorant compounds and methods of isolation of the colorant compounds derived from a reaction of genipin and an amine. The colorant compositions comprise purified compounds (e.g., a purified polymer or a purified dimer) obtained from multiple fractioning by chromatography of the reaction resulting material. The purified polymer or dimer can be used as a colorant by itself or in combination with another colorant for imparting color to a food, a drug, a cosmetic, a medical device, and textile products.

The invention provides novel genipin derivatives and a preparation method thereof. The novel genipin derivatives comprise geniposide derivatives and genipin derivatives. The invention belongs to the field of pharmaceutical chemistry. The geniposide derivative has a structure shown as a formula (I).

The invention belongs to the technical field of preparation of novel compounds, and particularly relates to an amide pentaacetyl geniposide derivative as well as a preparation method and application thereof. The amide penta-acetyl geniposide derivative provided by the invention is prepared by the following steps: firstly, hydrolyzing lipid groups at C-4 sites of iridoid in geniposide to obtain geniposidic acid, then acetylating five hydroxyl groups at 2 ', 3', 4 ', 6' and 11 sites on sugar to obtain penta-acetyl geniposidic acid (2a), and finally reacting with RNH2 to obtain the penta-acetyl geniposide derivative. The geniposide derivative prepared by the invention has remarkable XOD inhibitory activity and kidney injury improvement activity, and can effectively reduce the accumulation of uric acid in the kidney. The inflammatory reaction is reduced, so that the level of inflammatory factors is reduced; renal fibrosis of a mouse with hyperuricemia can be reduced by inhibiting expression of an inflammatory factor TGF-beta.

The invention provides an ink tattoo sticker based on a genipin coloring principle. The ink tattoo sticker comprises a paper base with a water-soluble sliding layer and release paper or film, and further comprises colorless viscose A arranged between the paper base and the release paper or film and colorless viscose B containing genipin, wherein the viscose A and the viscose B are sequentially overlapped and printed on the paper base by using a silk-screen printing technology on the paper base, and the release paper covers the paper base printed with the viscose A and the viscose B. Accordingto the ink tattoo sticker, the colorless viscose B and the viscose A with patterns are pasted on the surface of skin, the paper base is wetted by water, so that the viscose B and the viscose A with the patterns can be transferred to the skin, the genipin in the viscose B and amino acid in the skin of a human body generate a physical reaction, the patterns can be slowly displayed and gradually become blue black within 12-24 hours, the effect very close to that of a real tattoo is achieved, and the effect can be kept for 14 days or longer.

The invention belongs to the technical field of novel compound preparation, and particularly relates to an ester pentaacetyl geniposide derivative obtained through esterification reaction and a preparation method and application thereof. The amide penta-acetyl geniposide derivative provided by the invention is prepared by the following steps: firstly, hydrolyzing lipid groups at C-4 sites of iridoid in geniposide to obtain geniposidic acid, then acetylating five hydroxyl groups at 2 ', 3', 4 ', 6' and 11 sites on sugar to obtain penta-acetyl geniposidic acid (2a), and finally reacting with ROH to obtain the penta-acetyl geniposide derivative. The geniposide derivative prepared by the invention has remarkable XOD inhibitory activity and kidney injury improvement activity, and can effectively reduce the accumulation of uric acid in the kidney. The inflammatory reaction is reduced, so that the level of inflammatory factors is reduced; renal fibrosis of a mouse with hyperuricemia can be reduced by inhibiting expression of an inflammatory factor TGF-beta.