41 results about "Genipinine" patented technology

The invention is applicable to the technical field of biomedicine, and provides dipeptide-photosensitizer molecule co-assembled nanoparticles as well as a preparation method and application thereof. According to the invention, cationic diphenylalanine (CDP) and a biological cross-linking agent genipin are self-assembled to form a dipeptide carrier, and then the dipeptide carrier and a photosensitizer molecule chlorin e6 (Ce6) are co-assembled, so that nanoparticles with controllable size, controllable drug loading capacity and good biocompatibility are successfully prepared. The preparation method is simple and easy to implement, effectively solves the key problems that the traditional photosensitizer molecule Ce6 is poor in biocompatibility and easy to gather, not only can efficiently deliver Ce6, but also obviously improves the application safety and biocompatibility of Ce6. The Ce6 is almost free of dark toxicity under a dark condition, can play a strong photodynamic killing role under illumination, and greatly improves the possibility and application potential of the Ce6 in tumor photodynamic therapy by virtue of the flexible regulation and control advantages of the size and the drug loading capacity.

The invention relates to the technical field of biochemistry, in particular to a specific affinity enrichment and preparation method of a torreya grandis protease hydrolysate ACE inhibitory peptide, which comprises the following steps: step 1, preparing and characterizing magnetic aminated Fe3O4 nanoparticles; step 2, preparing and characterizing the immobilized trypsin; 3, preparing and characterizing the immobilized ACE, and optimizing reaction conditions; step 4, determining the activity of the immobilized ACE; step 5, then preparing and specifically enriching the Chinese torreya antihypertensive peptide; step 6, then, carrying out molecular docking through AutoDock Vina; according to the method, genipin is adopted as a cross-linking agent, magnetic Fe3O4 nanoparticles with the ACE inhibitory peptide specific affinity recognition function are constructed, specific enrichment of the ACE inhibitory peptide from the torreya grandis cake protein hydrolysate is achieved, and specific separation is achieved.

The invention discloses genipin modified ginkgo protein and whey protein composite gel and a preparation method thereof, and belongs to the field of functional food. The method comprises the following steps: (1) uniformly mixing a prepared ginkgo protein solution and a prepared whey protein solution, and adjusting the pH value to obtain composite sol; (2) adding a genipin reagent into the composite sol, carrying out a cross-linking reaction, and after the reaction is completed, adjusting the pH value again to obtain a genipin-treated composite sol; and (3) carrying out thermal induction gel formation on the genipin-treated composite sol, taking out and cooling to obtain the genipin-modified ginkgo protein and whey protein composite gel. Animal protein and plant protein are combined through the cross-linking agent genipin, so that the prepared composite gel is more nutritional, pollution to the environment is reduced, application in food production is facilitated, meanwhile, the composite gel treated with the cross-linking agent genipin has more diversified functional characteristics, and the composite gel is suitable for being applied to food production. And the food can be provided for people with different requirements.

The invention relates to a culture medium for separating and culturing stem cells in tissues and application of the culture medium. The culture medium is a basic culture medium containing genipin, plasmalogen and cell factors. The brand-new culture medium is designed, genipin, plasmalogen and cell factors are added into the basic culture medium and are integrally and synergistically matched, stem cells in tissues (such as nasal mucosa tissues) can be quickly activated, the adherence and migration speed of the stem cells is remarkably increased, proliferation of the stem cells is promoted, and cell activity is effectively maintained; the activation and separation efficiency of the stem cells is obviously improved.

The invention relates to a mitochondrial uncoupling inhibitor sustained-release nanodot, a preparation method thereof and application of the nanodot in type 2 diabetes mellitus, and belongs to the technical field of nano-drugs. According to the preparation method disclosed by the invention, glycine and genipin are subjected to Schiff reaction to prepare the slow-release nanodot, and the slow-release nanodot is of a monodisperse spherical structure, has surface negative charges, and can slowly release genipin under physiological conditions, so that the mitochondrial protection effect of the genipin is fully exerted. The mitochondrial uncoupling inhibitor slow-release nanodot prepared by the invention can obviously improve the mitochondrial membrane potential, increase the cell ATP content, effectively inhibit mtDNA release under the stimulation of IL-1beta and palmitic acid, effectively inhibit STING inflammation pathway activation under the stimulation of IL-1beta and PA, obviously reduce pancreatic beta cell apoptosis, obviously relieve endoplasmic reticulum stress, and can be used for preparing the mitochondrial uncoupling inhibitor slow-release nanodot. The T2DM mouse blood sugar is obviously reduced, the glucose tolerance dose and the insulin sensitivity are improved, and good biocompatibility is achieved.

The present disclosure relates to a use of Genipin-1-β-D-gentiobioside in preparation of a drug, specifically for use in a drug that treats respiratory inflammatory injury caused by coronavirus, respiratory syncytial virus, or Mycoplasma pneumoniae infection. The present disclosure has discovered that Genipin-1-β-D-gentiobioside possesses the characteristics of reducing viral load, lung index, and inflammatory cytokines in lung tissue following coronavirus infection, respiratory syncytial virus infection, or Mycoplasma pneumoniae infection. It also demonstrates a significant protective effect against death caused by coronavirus infection.

The invention relates to an application of genipin-1-beta-D gentiobioside in preparation of a medicine for treating chronic obstructive pulmonary disease. The genipin-1-beta-D gentiobioside is prepared into an aerosol inhalation solution, and the aerosol inhalation administration mode is adopted, so that the lung function in the chronic obstructive pulmonary disease can be improved, the content of inflammatory factors in lung tissues can be reduced, and the lung tissue injury can be relieved.

The present invention belongs to the field of organic synthesis technology and specifically relates to genipin derivatives, preparation methods, and applications thereof. The genipin derivatives provided by the present invention are structurally modified using genipin as a lead compound, attaching an unsaturated enol or alkynol group, and replacing the H atom on the C-1 hydroxyl group of genipin with 3-butene-1-ol, 4-pentene-1-ol, 5-hexene-1-ol, or 3-butyn-1-ol. The resulting four genipin derivatives containing unsaturated groups have excellent anti-inflammatory activity and are more suitable for preparing drugs for treating central nervous system inflammation.

The present invention discloses use of a male flower extract of Eucommia ulmoides Oliver in preparation of a food or a medicament for resisting muscle aging, belonging to the technical field of plant extracts. A main active ingredient of the male flower extract of Eucommia ulmoides Oliver is iridoids, and the composition of the iridoids includes aucubin, geniposide, geniposidic acid and asperuloside. The present invention reports for the first time that the male flower extract of Eucommia ulmoides Oliver and the iridoids contained therein have mitophagy activation activity and maintain normal mitochondrial function by effectively stimulating mitophagy. The extract has a relatively strong ability to prevent or alleviate aging-related muscle hypofunction, and has great application prospects in food, pharmaceutical and other fields.

A stabilized blue colorant is disclosed. The colorant includes a population of amino-acid-substituted genipin polymers having exclusively leucine, lysine, and arginine substitutions. The population includes a relative amino acid distribution of 5-90%leucine, 5-90%lysine, and 5-90%arginine. The stabilized blue colorant retains its blue color better than existing options. The colorant is made by a process that includes reacting genipin with the specific amino acids identified.

The invention relates to a genipin-based skin metabolism visual evaluation agent and application thereof, and belongs to the technical field of cosmetics. The invention provides a skin metabolism visualization evaluation agent which comprises the following components in parts by mass: 1-2 parts of genipin, 0.4-0.7 part of a coloring agent and 0.1-0.5 part of a thickening agent, and the pH value of the skin metabolism visualization evaluation agent is 6.0-6.5. The genipin, the coloring agent and the thickening agent are combined into the skin metabolism visual evaluation agent, so that the skin can be effectively dyed, the safety is relatively good, and the metabolism condition of the skin can be more intuitively and accurately evaluated. Meanwhile, the skin metabolism visualization evaluation agent is high in adhesive force on the skin, better in stability and material uniformity and more uniform in dyeing effect, can be used in people with deep skin color and light skin color, and is not limited by a test area.

The invention belongs to the technical field of biological material preparation, and provides genipin cross-linked black bean peptidyl tea polyphenol sustained-release gel balls and a preparation method thereof. The preparation method comprises the following steps: dissolving black bean protein in water, carrying out microwave treatment, heating and incubating, adding protease for enzymolysis modification, and carrying out enzyme deactivation and precipitate removal to obtain a black bean peptide solution; the preparation method comprises the following steps: uniformly mixing black soybean peptide with a sodium alginate solution, adding tea polyphenol, uniformly stirring and mixing, adding a genipin aqueous solution, carrying out constant-temperature water bath, and dropwise adding the obtained solution into a calcium chloride solution to form gel balls; the prepared genipin cross-linked black bean peptidyl tea polyphenol sustained-release gel ball has a compact and stable wrinkled three-dimensional network structure and is high in biocompatibility and dispersity, and the stability of tea polyphenol is remarkably improved; the pH-responsive tea polyphenol slow release performance is achieved, accurate slow release of the tea polyphenol in intestinal juice is achieved, and the bioavailability of the tea polyphenol is improved.

Embodiments described herein include semi-permanent inks (e.g., semi-permanent inks contains genipin, a genipin derivative, lawsone, a lawsone derivative, or combinations thereof); semi-permanent ink applicators; transfer mediums; silicone adhesives; and silicone mediums and kits containing two or more thereof. Some embodiments describe methods for applying an ink to skin as well as methods for increasing the darkness of a semi-permanent tattoo and / or increasing the longevity of a semi-permanent tattoo.

The application discloses a capillary electrophoresis quantitative analysis method of active ingredients of Wuobi Shanyao pills. The method comprises the following steps: pretreating and purifying the powder of the test sample Wuobi Shanyao pills by MSPD-SPE, and quantitatively determining the active ingredients of the test sample, i.e., loganin, geniposidic acid, trilobatin and verbascoside by CE-UV. The MSPD with high extraction efficiency, the SPE with strong purification capacity and the CE with less consumption of organic solvents are combined for the first time, and are used for green, efficient and accurate content determination of the four components (loganin, geniposidic acid, trilobatin and verbascoside) in Wuobi Shanyao pills. In the sample preparation process and in the CE separation and determination, the consumption of organic solvents is far less than the amount consumed in the prior art.

The invention belongs to the field of preparation of functional compounds, and particularly relates to a genipin derivative with an amino ether structure, a preparation method of the genipin derivative and application of the genipin derivative to reduction of uric acid. The genipin derivative is designed and synthesized by taking genipin as a lead compound and carrying out chemical modification on the genipin. In order to enhance the binding capacity of the genipin derivative and XOD, the C-14 site of genipin ether is substituted, an aromatic ring or aromatic heterocyclic group containing organic amine is introduced, and the chemical structure of the obtained genipin derivative is represented and confirmed through 1H NMR and 13C NMR. The geniposide derivative synthesized by the invention is subjected to XOD inhibitory activity screening. The invention provides a structural and theoretical basis for subsequent further research on a more efficient XOD inhibitor.

The invention relates to a medicine for inhibiting breast cancer angiogenesis, and discloses an application of genipin in preparation of the medicine for inhibiting breast cancer angiogenesis. Research results of the invention show that the genipin can directly inhibit expression of HIF-1alpha so as to interfere hypoxia-related pathways and prevent hypoxia-related transcription factors from playing a role. In addition, the genipin can also reduce the expression of HSPG2, so that the accumulation of extracellular matrix components is reduced, and cell proliferation, migration and angiogenesis damage are finally caused, and therefore, the genipin can be used for preparing the medicine for inhibiting breast cancer angiogenesis.

The application provides a genipin cross-linked selenium nanoparticle and a preparation method and application thereof, and belongs to the field of functional nanomaterials and intelligent nutrition delivery technology. The preparation method comprises the following steps: dissolving a protein substance in water to obtain a protein solution, adding a selenous acid compound, then adding vitamin C and genipin, performing cross-linking reaction, and performing dialysis on the obtained reaction solution to obtain the genipin cross-linked selenium nanoparticle. The application innovatively uses the concentration of genipin as a control switch, realizes active and accurate control of the release rate of selenium in the intestinal tract, realizes the treatment effect on colitis through instantaneous high-concentration release under a low-concentration selenium administration dose, and effectively solves the defect that the "nutrition-toxicity" dose range of selenium is narrow.

The invention discloses a pharmaceutical composition and application thereof. The pharmaceutical composition provided by the invention comprises the following active components in percentage by weight: 10-50% of geniposide, 10-50% of a surfactant, and 10-50% of a 10% to 50% of geniposide; 10% to 50% of genipin gentiobioside; 10 to 50 percent of crocin I and 10 to 50 percent of ursolic acid. The composition can effectively inhibit paralysis of tremor, improve the balancing capacity and grasping capacity of mice, reduce the content of interleukin-1beta and tumor necrosis factor-alpha, promote secretion of dopamine, reduce the content of malondialdehyde and improve the total antioxidant capacity in brain tissue. The compound has a good treatment effect on a Parkinson's disease model, so that the effect of treating the Parkinson's disease is expected to be greatly improved, and adverse reactions are reduced.

The invention discloses thermophilic beta-1, 4-glucosidase and an application of the thermophilic beta-1, 4-glucosidase. The base sequence of the thermophilic beta-1, 4-glucosidase is as shown in SEQ ID NO: 1. The thermophilic beta-glucosidase provided by the invention can be used for hydrolyzing p-nitrophenyl-beta-D glucopyranoside (pNPGlu), p-nitrophenyl-beta-D galactopyranoside (pNPGal), p-nitrophenyl-beta-D xylopyranoside (pNPXyl), p-nitrophenyl-alpha-D glucopyranoside (pNP alpha Glu), p-nitrophenyl-N-acetylglucosamine, geniposide, polydatin, glucosyltransferase, glucosyltransferase and the like. And salicin and arbutin. The optimum reaction temperature of the enzyme is 95 DEG C, and after the enzyme is treated at 70 DEG C for 24 hours, the residual enzyme activity is greater than 40%. In an n-hexyl alcohol-water phase (or n-caprylic alcohol-water phase), geniposide can be hydrolyzed into genipin, and industrial production of genipin is facilitated.

Use of genipin-1-β-D gentiobioside in the preparation of a drug for treating chronic obstructive pulmonary disease. Genipin-1-β-D gentiobioside is prepared into a solution for atomized inhalation. Using the administration mode of atomized inhalation can improve lung function in chronic obstructive pulmonary disease, reduce the content of inflammatory factors in lung tissue, and reduce damage to lung tissue.

A temporary tattooing ink is produced from concentrated genipin. In one embodiment, the concentrated genipin forms part of a solution. In another embodiment, the genipin is provided in a gel form which also includes a solvent and a thickening agent. Finally, an applicator is described into which genipin may be embedded for applying to a user's skin.

The use of genipin-1-β-D-gentiobioside in preparing drugs, comprising the use in drugs for treating respiratory inflammatory injuries caused by infections of coronaviruses, respiratory syncytial viruses or mycoplasma pneumoniae. The genipin-1-β-D-gentiobioside has the characteristics of reducing the viral load, lung index and lung tissue inflammatory cytokines in lung tissue after infections of coronaviruses, respiratory syncytial viruses or mycoplasma pneumoniae, and obviously preventing death caused by coronavirus infections.

The invention discloses a preparation method and medical application of genipin derivatives, and a series of genipin derivatives are obtained by structurally modifying active sites of a natural compound genipin. The genipin derivative provided by the invention has very strong anti-hepatic fibrosis activity, and can effectively inhibit the up-regulation of hepatic fibrosis markers; and the genipin derivative can play a role by inhibiting a TGF-beta / Smad signal channel. The invention discloses an application of the genipin derivative or the pharmaceutically acceptable salt and solvate thereof in preparation of a medicine for treating hepatic fibrosis.

The invention belongs to the field of preparation of functional compounds, and particularly relates to a methylated aromatic substituted genipin derivative with a uric acid reducing effect as well as a preparation method and application of the methylated aromatic substituted genipin derivative. The genipin derivative synthesized by the invention is subjected to XOD inhibitory activity screening, and researches on in-vivo and in-vitro activity, uric acid reduction and XOD inhibitory activity, anti-inflammatory activity and anti-fibrosis activity show that the derivative shows relatively high XOD inhibitory activity; oxidative stress is relieved by inhibiting XOD activity at the liver, so that liver inflammation is inhibited. The invention provides a structural and theoretical basis for subsequent further research on a more efficient XOD inhibitor.

The invention discloses genipin derivatives as well as a preparation method and application thereof. According to the invention, genipin is subjected to chemical structure transformation and modification, and nitrochloroacetyl chloride bullet is introduced into the structure of genipin, so that the anticancer activity of the compound is improved. The genipin derivative disclosed by the invention has very strong anti-tumor activity, can effectively inhibit proliferation of tumor cells, and has no obvious toxicity to normal cells; the genipin derivative disclosed by the invention can play a role by inducing ferroptosis, and is a novel ferroptosis inducer for inhibiting tumor cell proliferation.

According to the oleic acid-geniposide composite preparation and the application thereof, a mixed system of oleic acid and geniposide is constructed and acts on PC12 cells induced by hydrogen peroxide to generate oxidative damage, and the result shows that compared with cells treated by singly using geniposide with the same concentration, the oleic acid-geniposide composite preparation has the advantages that the effect is good, and the cost is low. The mixed system can obviously improve the survival rate of PC12 cells, and the obvious effect is not observed after linoleic acid and linolenic acid are respectively mixed with geniposide. The invention clarifies that oleic acid can enhance the protective effect of geniposide on oxidative damage cells, and provides a brand new thought and technical support for developing products for resisting oxidative damage of cells.

The present application relates to topical compositions comprising a compound capable of covalently binding to the skin and an excipient suitable for topical application wherein the compound capable of covalently binding to the skin is a compound of formula (I) or a tautomer and / or a pharmaceutically acceptable salt thereof, wherein R1 represents hydrogen or a protecting group that is hydrolysable under physiological conditions upon application of the topical composition to the skin; wherein L1, L2 and L3, independently of each other, represent a linking group or are absent, and wherein A1, A2 and A3, independently of each other, represent: a) a C1-C30 moiety, H, hydroxyl, amino or halogen, or b) a C1-C60 moiety or a polymeric moiety; wherein at least one of A1, A2 and A3 is a C1-C60 moiety or a polymeric moiety of group b); wherein the C1-C60 portion or polymeric portion of group b): b1) is configured to act as a skin moisturizer, an insecticide, a skin whitening agent or a drug; and / or b2) is configured to act as a skin moisturizer, an insecticide, a skin whitening agent, a fragrance or a drug upon cleavage from the 3, 4-dihydro-2H-pyran moiety; and wherein L1-A1 and L2-A2 do not represent a moiety comprising an unsaturated C-C or C-N bond at the alpha position of the carbon atoms marked as "a" and "b", respectively. (I);

The invention relates to the technical field of medical materials, in particular to a coloring agent for endoscopic surgery and a preparation method and application thereof.The coloring agent is an acetyl-GPLGIRG-genipin enzyme activated precursor, the acetyl-GPLGIRG-genipin enzyme activated precursor is obtained through a reaction of acetylated GPLGIRG polypeptide and genipin, and the GPLGIRG polypeptide and the genipin are used for preparing the coloring agent for endoscopic surgery. The genipin is connected to the carbon end of the acetylated GPLGIRG polypeptide. Rapid, clear and lasting targeted blue labeling of a lesion area is realized by using a specific cross-linking reaction of a genipin component and a protein amino group and utilizing biochemical difference of a lesion tissue and a normal tissue on a microstructure. The problem that a traditional staining agent cannot specifically mark lesions is solved, a pad formed by lifting liquid under mucous membranes is more stable and durable due to the cross-linking characteristic of the staining agent, and double advantages are provided for endoscopic surgery.

The present invention discloses a bis - amino acid - genipin derivative, its preparation method and application; aiming to provide a bis - amino acid - genipin derivative with neuroprotective activity; the hemiacetal at the C - 1 position of genipin is oxidized and aminated to introduce the first amino acid fragment, and the second amino acid is introduced by esterification at the C - 10 position to obtain a series of brand - new genipin derivatives, which show good neuroprotective activity in the OGD / R - induced HT22 nerve cell injury model and can be used for preparing neuroprotective drugs; the general structural formula of the bis - amino acid - genipin derivative is as follows; it relates to the fields of pharmaceutical technology and medicinal chemistry.

The invention discloses a double amino acid-genipin derivative as well as a preparation method and application thereof. The invention aims to provide a bis-amino acid-genipin derivative with neuroprotective activity. A first amino acid fragment is introduced through oxidation and amination of hemiacetal at the C-1 position of genipin, a second amino acid fragment is introduced through esterification at the C-10 position to obtain a series of brand-new genipin derivatives, and the genipin derivatives show good neuroprotective activity on an OGD / R induced HT22 nerve cell injury model and can be used for preparing neuroprotective drugs; the structural general formula of the double amino acid-genipin derivative is # imgabs0 #, relates to the field of medical technology and medicinal chemistry.