4 results about "GTP-sepharose" patented technology

The utility model belongs to the technical field of sepharose gel preparation, and particularly relates to a temperature measuring and blending device for sepharose gel preparation, which comprises an oscillating and blending device, the oscillating and blending device comprises a vibrating plate capable of vibrating, a fixing device for fixing a preparation container is clamped on the vibrating plate, and a temperature measuring device is arranged on the vibrating plate. The fixing device is fixedly connected with a temperature measuring plate for placing a preparation container, and a thermochromic film is arranged on the upper surface of the temperature measuring plate. Compared with the prior art, by arranging the fixing device and the temperature measuring plate on the fixing device, the technical problems that in the prior art, an agarose solution needs to be shaken up manually, and the agarose solution is possibly polluted when a traditional temperature measuring device is used are solved.

The invention provides a preparation method of an aprotinin affinity chromatographic column. Specifically, the aprotinin is used as a raw material and is coupled with CNBr activated agarose gel Focurose 4FF to prepare the aprotinin affinity chromatographic column. The coupling reaction in the preparation method is simple and easy to control, the reaction condition is mild, the operation is convenient, the repeatability is high, and industrial amplification is easy. The affinity column prepared by the invention can completely remove human urinary kallidinogenase in urokinase, and can be repeatedly used for multiple times.

The invention relates to the technical field of polymer glycosylation analysis, and discloses a VWF polymer glycosylation detection method based on gel protein electrophoresis, and the method comprises the following steps: carrying out denaturation treatment on VWF-rich plasma, platelet lysis buffer or cell supernatant to prepare a sample; the method comprises the following steps: carrying out low-temperature constant-pressure electrophoresis on a sample by adopting agarose gel protein electrophoresis, separating VWF polymers with different glycosylation degrees, transferring colloids after electrophoresis to a hydrophilic elastic polyester film, and carrying out air drying to prepare a plurality of colloids; lectins are sequentially added into the colloids, different colloids correspond to different lectins in a one-to-one mode, and then streptavidin-peroxidase is added for incubation; and after incubation is finished, smearing the chemiluminescence liquid on the colloid, and determining the glycosylation type and quantifying the glycosylation abundance of the luminescence product through an imaging system. According to the present invention, the rapid detection of the VWF glycosylation mode is achieved, the sugar chain coverage range is expanded, the detection specificity is improved, and the observability of the sugar chain signal is improved.