The invention provides a multi-PCR kit for rapidly identifying Dragonfish type and an identifying method thereof; the kit includes 1, three upstream primers and three downstream primers; 2, positive comparison product; 3, PCR reaction fluid; meanwhile, the method also provides a method for rapidly identifying the Dragonfish type, wherein the method includes steps of 1), selecting three upstream primers and three downstream primers; 2), preparing a
gene group
DNA template; 3), respectively adding the upstream primers and the downstream primers in the
gene group
DNA template prepared by the positive controlled
DNA template and the Dragonfish to be tested, so as to perform PCR amplified reaction;4). Taking 5 miu L products after amplification and applying samples to 2.5% of
sepharose gel; taking 50 bp Marker (stripes 50, 100, 150, 200, 250, 300, 350, 400, 450, 500) as standard molecule reference and performing
electrophoresis; applying the
electrophoresis result to the analysis of an
ultraviolet gel imaging
system; 5), judging result. The kit and the method can improve the identifying efficiency, save time, and save the identifying cost.