7 results about "GTP-sepharose" patented technology

The utility model belongs to the technical field of sepharose gel preparation, and particularly relates to a temperature measuring and blending device for sepharose gel preparation, which comprises an oscillating and blending device, the oscillating and blending device comprises a vibrating plate capable of vibrating, a fixing device for fixing a preparation container is clamped on the vibrating plate, and a temperature measuring device is arranged on the vibrating plate. The fixing device is fixedly connected with a temperature measuring plate for placing a preparation container, and a thermochromic film is arranged on the upper surface of the temperature measuring plate. Compared with the prior art, by arranging the fixing device and the temperature measuring plate on the fixing device, the technical problems that in the prior art, an agarose solution needs to be shaken up manually, and the agarose solution is possibly polluted when a traditional temperature measuring device is used are solved.

The invention provides a preparation method of an aprotinin affinity chromatographic column. Specifically, the aprotinin is used as a raw material and is coupled with CNBr activated agarose gel Focurose 4FF to prepare the aprotinin affinity chromatographic column. The coupling reaction in the preparation method is simple and easy to control, the reaction condition is mild, the operation is convenient, the repeatability is high, and industrial amplification is easy. The affinity column prepared by the invention can completely remove human urinary kallidinogenase in urokinase, and can be repeatedly used for multiple times.

The invention relates to a polymer grafted ion exchange chromatography medium for separating recombinant human serum albumin and a preparation method thereof. The preparation method comprises the following steps: by taking agarose as a raw material and calcium carbonate as a pore-foaming agent, synthesizing porous agarose gel matrix microspheres by adopting an emulsification-curing method; coupling functional ligands of diethylaminoethyl and polyethyleneimine to prepare a polymer grafted anion exchange medium; the method is applied to efficient separation and purification of an actual protein system, and the adsorption capacity and adsorption efficiency of protein can be improved. Experiments prove that compared with a commercialized non-grafted anion exchange chromatography medium Q Sepharose FF, the DEAE coupled PEI polymer grafting medium taking the porous agarose microspheres as a matrix shows higher static adsorption capacity, and the moving target protein recovery rate reaches 89.6%. The medium is simple in preparation process, low in cost and good in biocompatibility, and has a good application prospect in efficient and rapid separation and purification of protein.

The invention relates to the technical field of polymer glycosylation analysis, and discloses a VWF polymer glycosylation detection method based on gel protein electrophoresis, and the method comprises the following steps: carrying out denaturation treatment on VWF-rich plasma, platelet lysis buffer or cell supernatant to prepare a sample; the method comprises the following steps: carrying out low-temperature constant-pressure electrophoresis on a sample by adopting agarose gel protein electrophoresis, separating VWF polymers with different glycosylation degrees, transferring colloids after electrophoresis to a hydrophilic elastic polyester film, and carrying out air drying to prepare a plurality of colloids; lectins are sequentially added into the colloids, different colloids correspond to different lectins in a one-to-one mode, and then streptavidin-peroxidase is added for incubation; and after incubation is finished, smearing the chemiluminescence liquid on the colloid, and determining the glycosylation type and quantifying the glycosylation abundance of the luminescence product through an imaging system. According to the present invention, the rapid detection of the VWF glycosylation mode is achieved, the sugar chain coverage range is expanded, the detection specificity is improved, and the observability of the sugar chain signal is improved.

According to the hucho taimen roe micro-injection device and method, an injection pipe and a discharge pipe are arranged side by side and integrally manufactured, needle tips are arranged at the lower ends of the injection pipe and the discharge pipe, and a liquid storage tank is communicated with the discharge pipe through a connecting pipe. The method comprises the following steps: 1, semen taking; 2, healthy roes are taken, and a container containing the roes is placed on the ice-water mixture; 3, mixing 1000 + / -10 eggs with the seminal fluid, gently and uniformly stirring, and slowly adding water until the eggs are submerged; 4, standing for 2 minutes, washing the eggs with water at 10 DEG C, and removing redundant sperms; 5, injecting clean water again; 6, pouring 1.2% sepharose gel into a culture dish, and after the sepharose gel is coagulated, poking out a semicircular groove with the diameter of 4-5mm; seventhly, the fertilized eggs absorb water to expand, within 0-15 minutes after meeting water, the fertilized eggs are fixed in the semicircular grooves, and injection is conducted through a micro-injection device; 8, timely picking out the eggs which are damaged, severely leaked, not fertilized or have water mildew infection symptoms; and 9, incubating the fertilized eggs after injection. The microinjection device is used for microinjection of hucho taimen fertilized eggs.

The present invention provides a responsive double-network hydrogel and its preparation method and application, which relate to the field of nucleic acid separation and recovery. This hydrogel is composed of an agarose gel network and a poly (N-isopropylacrylamide) network. By adjusting the ratio of agarose and poly (N-isopropylacrylamide), the separation resolution of nucleic acids during electrophoresis can be enhanced, and the separation range of nucleic acids of different molecular weights can be regulated. The temperature sensitivity of poly (N-isopropylacrylamide) enables the double-network hydrogel to have thermal responsiveness. When heated above its lower critical phase transition temperature (~32°C), the double-network hydrogel can squeeze out the aqueous phase in the gel through the phase transition of poly (N-isopropylacrylamide), thereby releasing the nucleic acids therein without destroying the hydrogel network, simplifying the nucleic acid recovery operation, and improving the nucleic acid recovery efficiency and sample recovery rate. This method is suitable for the separation and recovery of various nucleic acid fragments and nucleic acid-assembled nanostructures.