9 results about "Hydroxylamine derivatives" patented technology

The invention belongs to the technical field of biological medicine, and discloses a preparation method of ionizable cationic lipid. The preparation method comprises the following steps: reacting 7-oxoheptyl caprate with hydroxylamine or hydroxylamine salt in the presence of alkali in the presence of a reducing agent and a cobalt-ruthenium alloy catalyst; (9Z, 12Z)-octadecane-9, 12-dienal is added into the reaction product, the reaction continues in the presence of a reducing agent, and a hydroxylamine derivative intermediate is obtained; and performing amidation reaction with 4-dimethylaminobutyric acid or hydrochloride thereof in the presence of a condensing agent and a catalyst to obtain the ionizable cationic lipid. The invention also discloses an application of the ionizable cationic lipid, the lipid nanoparticle composition or the mRNA-loaded lipid nanoparticles obtained by the preparation method in preparation of a pharmaceutical composition or a kit for mRNA delivery. The LNP provided by the invention has good physicochemical properties, biocompatibility and efficient mRNA encapsulation and intracellular delivery capabilities, and can be applied to preparation of pharmaceutical compositions or kits for mRNA delivery.

The present invention belongs to the technical field of fluorescent probes, and specifically relates to a formaldehyde fluorescent probe based on formaldehyde-induced catalytic hydrolysis of aminocarboxylic acid hydroxylamine esters, a preparation method thereof, and applications thereof. The preparation of the formaldehyde fluorescent probe specifically comprises the following steps: S1: a naphthalimide derivative A is refluxed with triphosgene and 4-dimethylaminopyridine in a toluene solution to obtain an intermediate B; S2: the intermediate B is reacted with a hydroxylamine derivative C in a dichloromethane solution at room temperature to obtain a probe compound I; the molar ratio of the naphthalimide derivative A, triphosgene, and 4-dimethylaminopyridine in S1 is 1:1.5:3; the molar ratio of the intermediate B to the hydroxylamine derivative C in S2 is 1:1.3; the fluorescent probe of the present invention can selectively and rapidly react with formaldehyde to generate a product with strong fluorescence, and exhibits high selectivity and sensitivity to formaldehyde.

The invention relates to the technical field of medicines, and discloses a Horsfiquione B derivative as well as a preparation method and application thereof, the applicant of the invention finds that Horsfiquione B hydroxylamine derivatives 1 and 2 are prepared by adopting a reaction between Horsfiquione B and hydroxylamine, and finds that the anti-tumor activity of the Horsfiquione B hydroxylamine derivatives is doubled, and the Horsfiquione B hydroxylamine derivatives have remarkable anti-breast cancer and leukemia tumor cell proliferation activity. The application prospect of tumor treatment or adjuvant treatment is realized.

The invention discloses a real-time reaction monitoring method and a real-time reaction monitoring system for DNA (Deoxyribose Nucleic Acid) base removal sites and small molecule toxic compounds, and belongs to the technical field of biological analysis. The method comprises the following steps: constructing a compound formed by combining a uracil-containing DNA probe and an electrochemical active marker, and hydrolyzing uracil by using UDG enzyme to form a base removal site. A nanopore single-molecule detection technology is adopted, a compound is driven by voltage to pass through a hole, and a characteristic current signal is generated to establish a baseline map. Hydroxylamine derivatives are introduced to react with base removal sites, and dynamic interaction is analyzed according to time domain change of current signals. The system comprises a labeled compound preparation module, an enzymatic reaction module, a nanopore detection module, a small molecule compound reaction module and a central control and data processing module, all the modules cooperate, full-process automation from probe preparation to dynamic reaction monitoring is achieved, traditional sensitivity limitation is broken through, and a high-precision detection means is provided for studying the DNA damage and toxic compound action mechanism.

The present application belongs to the technical field of medicine, and relates to a diphenyl hydroxylamine derivative shown in general formula I, a stereoisomer and a pharmaceutically acceptable salt, a hydrate, a solvate or a prodrug thereof, wherein the substituent groups X, Ar and R have the definitions given in the specification. The present application also relates to a method for preparing the compound of general formula I, a pharmaceutical composition containing the compound, and the use of the compound and the pharmaceutical composition in the preparation of a medicament for treating and preventing diseases caused by superficial bacterial and deep Gram-negative bacterial infections.

The invention discloses a preparation method of a hydroxylamine derivative, under the action of a catalyst, a hydroxylamine aqueous solution and a functional group-containing compound react to obtain the hydroxylamine derivative, the catalyst is a basic catalyst, and the molar ratio of the functional group-containing compound to hydroxylamine in the hydroxylamine aqueous solution is 1: (1-2); the molar ratio of the functional group-containing compound to the basic catalyst is 1: (0.001-0.5). The method has the advantages of good catalytic activity, high reaction efficiency, easiness in separation of the catalyst, and greenness and environmental protection.

The invention provides a low-cost and high-plating-speed pure palladium chemical plating solution for a power chip, which comprises the following components based on the total amount of the plating solution: 0.4-0.6 g / L of water-soluble palladium salt, 20-30 g / L of composite complexing agent, 10-20 g / L of pH buffer agent, 8-12 g / L of double reducing agent, 0.1-0.5 g / L of accelerator and 5-8 mg / L of stabilizer, the composite complexing agent comprises 5-10 g / L of organic amines and 15-20 g / L of amino carboxylic acid; the double reducing agents are selected from two of hydrazine substances, formic acid substances or hydroxylamine substances. The chemical palladium plating solution disclosed by the invention can be used for high-speed pure palladium plating in an aluminum substrate chemical nickel-palladium-gold plating surface treatment process on a power chip, an obtained plating layer is a phosphorus-free pure palladium plating layer, the plating speed of the plating solution under the condition of lower palladium content reaches 0.049 mu m / min, the liquid medicine cost and the time cost in the production process can be effectively reduced, and the production efficiency is improved. And meanwhile, the plating solution further has good stability, and stable operation of five MTOs can be achieved.

The invention discloses a method for synthesizing 9H-tribenzo[B,D,F]azepine, and relates to the technical field of chemical synthesis of organic luminescent materials. The method aims to solve the problem that most existing synthesis methods require a Pd catalyst and are not simple and efficient. The method comprises the following steps: using 9,10-triphenylene as a basic reaction raw material, a hydroxylamine derivative as an amine source, and FeBr3, CuBr2 or CoBr2 as a catalyst; reacting in a reaction solvent to achieve ring expansion and nitrogenation under aromatic group migration; and obtaining a 9H-tribenzo[B,D,F]azepine product through separation and purification. The method has low raw material price, simple and easy operation of the synthesis steps, mild and easy-to-control reaction conditions, a low-cost and low-toxic catalyst, and an environmentally friendly preparation process. The yield of the 9H-tribenzo[B,D,F]azepine product is high, and the method is suitable for industrial application.

The present application mainly relates to the efficient synthesis of a kind of tri-substituted hydroxylamine compound by transition metal Pd catalytic C-O bond coupling.The present application realizes the one-pot reaction of tertiary chloroalkane or secondary chloroalkane and O-acyl hydroxylamine compound under the action of catalyst, ligand and base in argon atmosphere, realizes C-O bond coupling in the case of keeping the fragile N-O bond unbroken, and obtains a kind of big steric hindrance N,N,O-trialkyl substituted hydroxylamine derivative with high chemical selectivity.The present application has the characteristics of excellent chemical selectivity to construct C-O bond, simple experimental operation, wide source of raw materials, high yield, wide substrate application range and excellent functional group tolerance, and the synthesized product is suitable for being contained in drug small molecule screening library for the discovery of lead drug.