37 results about "Infranatant" patented technology

The invention discloses a method for simultaneous extraction and analysis of a metabolite group and a lipid group in microtissue. The method comprises the following steps: freeze drying to-be-analyzed microtissue, accurately weighing 1 to 25 mg of the freeze-dried microtissue and adding solvents like methanol (MeOH), methyl tert butyl ether (MTBE) and water in certain proportion for extraction; allowing a solution obtained after completion of extraction to be divided into two layers, wherein an upper layer mainly contains nonpolar metabolites and lipids, and the lower layer mainly comprises polar and medium-polar metabolites; and subjecting the upper-layer solution and the lower-layer solution to mixing in proportion and freeze-drying, then carrying out redissolving and then metabonomical analysis based on liquid chromatography-mass spectrometry, subjecting the upper-layer solution to freeze-drying and then to redissolving and carrying out lipidomical analysis based on liquid chromatography-mass spectrometry. The method has the following advantages: metabolites and lipids are extracted as many as possible through one extraction of a small amount of tissue, and through metabonomical and lipidomical analysis, the amount of a tissue sample is saved, which benefits other biochemical analysis of the tissue sample.

The invention belongs to the technical field of poultry product detection and relates to a combined detection method of amantadine, rimantadine, ribavirin and moroxydine residues in eggs. The method comprises the following steps: carrying out low temperature repetitive freeze-thawing on a sample, adding a formic acid-methanol solution, mixing, centrifuging, taking a supernatant for standby, adding water saturated n-hexane, mixing, carrying out ultrasonic treatment, centrifuging to remove n-hexane floccules on the upper layer with an extracting solution left at the lower layer, purifying with a cation exchange solid-phase extraction column, measuring by a liquid chromatography-tandem mass spectrometer provided with an ESI source, and carrying out accurate qualitative and quantitative analysis on residues of the four antiviral drugs in eggs. The method provided by the invention has high specificity, can accurately and simultaneously measure residual quantity of amantadine, rimantadine, ribavirin and moroxydine without pollution, has high sensitivity, and provides technical support for guaranteeing quality safety of eggs.

The invention discloses a liquid-mass method for detecting various marine biotoxins in an aquatic product. The sample of the aquatic product to be tested is extracted by using 0.1 vol% formic acid aqueous solution and acetonitrile in a classification way repeatedly, and layering is carried out to acquire upper organic phase and lower aqueous phase. The lower aqueous phase is added into an MCX-HLBseries column. Rinsing is carried out with a low proportion of methanol water. Elution is carried out with the organic phase purified by dSPE. Secondary elution is carried out with alkaline methanol.A high performance liquid chromatography-tandem mass spectrometer is used for detection after adding eluant with a dropper to bring the bottom of the meniscus to the line. An external standard methodis used for quantification to acquire the content of each component to be tested in the sample of the aquatic product to be tested. According to the invention, eight kinds of hydrophilic and lipophilic marine biotoxins can be detected at one time, and first-step liquid-mass high-throughput quantitative detection of hydrophilic and lipophilic marine biotoxins is realized.

The invention relates to a biochemical index detection integrated chip comprising an upper layer and a lower layer; the upper layer and the lower layer are connected in a water tightness manner, the upper layer is provided with a sample through hole, the lower layer comprises a sample tank, a liquid storage tank, a sample waste liquid tank, a sample quantification tank and a micro-reflection surface, and the tanks are connected through flow channels; the sample waste liquid tank is characterized in that the sample waste liquid tank comprises a first waste liquid tank and / or a second waste liquid tank or two or more waste liquid tanks, and freeze-drying reaction reagents are preloaded in the first waste liquid tank and / or the second waste liquid tank or two or more waste liquid tanks. Whenthe chip is used for detecting the pH value, the cost and the consumption of biological samples and reagents can be greatly reduced, and meanwhile, the defect that the pH value cannot be detected through micro-fluidic in-vitro rapid diagnosis is overcome.

The invention discloses a device and a method for recovering a chromatographic grade organic reagent from an organic reagent waste liquid. The device comprises an organic reagent waste liquid salting-out tank, an organic reagent distillation kettle, an organic reagent pervaporation membrane separation device and a saturated salt solution distillation tank. The method comprises the following steps: the organic reagent waste liquid enters the organic reagent waste liquid salting-out tank; after the organic reagent waste liquid is added into the organic reagent waste liquid salting-out tank from the saturated salt solution distillation tank according to a certain proportion, carrying out sufficient stirring and standing; after stratification, a supernviaould like liquid returns to the saturated salt solution distillation tank for distillation; after the distillation, a saturated salt solution is obtained for repeated use; a supernatant organic solvent liquid is transferred to the organic reagent distillation kettle. Through the adoption of the device, a chromatographic grade organic reagent waste liquid can be collected; the chromatographic grade organic reagent, namely an effective constituent in the waste liquid, can be recovered by certain means, such as the coupling between salting-out and dewatering, the coupling between distillation and impurity removing, and the coupling between pervaporation and liquid-liquid separation; the recovered chromatographic grade organic reagent can reach the national standard.

The invention relates to a method of determining risedronate in human plasma based on LC-MS / MS pre-derivatization. The pre-derivatization comprises steps: 150 muL of a plasma sample is taken, 5.00 muLof an internal standard working solution is added, uniform mixing is carried out, 150 muL of 10% trichloroacetic acid is added, after 3 min of oscillation, centrifugation for 5 min at 13,000 rpm is carried out, 100 muL of a supernatant is taken, 57.0 muL of methanol-25% ammonia water (50 to 7, v / v) is added, and 3 min of oscillation is carried out; and 400 muL of trimethylsilyl diazomethane is added, oscillatory derivation for 4 h at 1600 rpm is carried out, and after standing, 2.00 muL of a lower solution is taken for sampling. In comparison with the prior art, a derivative reaction is directly carried out after protein precipitation, the risedronate sample preprocessing process is greatly simplified, and the method has the advantages of short running time, good selectivity and high sensitivity.

The invention discloses a pretreatment method and a quantitative detection method of quinolone antibiotics in a biological sample. The method is characterized by comprising the steps of putting a biological sample to be detected into a glass centrifuge tube, adding a 10 mmol / L phosphate buffer solution of which the volume is 10 times that of the sample liquid to be detected and the pH value is 6,and uniformly mixing to obtain a uniform solution; adding a TiO2-Tb nano material into a uniform solution; performing ultrasonic-assisted extraction for 3 to 8 minutes; centrifuging and discarding anupper-layer solution; adding an acetic acid solution with the mass concentration of 4wt% into a lower-layer solution, wherein the volume of the acetic acid solution is equal to that of the phosphate buffer solution; and performing ultrasonic-assisted back extraction for 12 to 18 minutes, centrifuging, taking an upper-layer solution, filtering with a 0.45 mu m microporous filter membrane, and applying the upper-layer solution to high performance liquid chromatography-ultraviolet spectrophotometric quantitative detection of quinolone antibiotics. The method has the advantages of simplicity, quickness, high target recovery rate, high sensitivity and good accuracy.

The invention discloses a preparation method of an allograft-derived platelet-rich gelator. The method comprises the steps as follows: (1) umbilical cord blood collection; (2) primary centrifugation:centrifuging a blood sample at the centrifugation parameter of 1500 r / min for 10 min; sucking a supernatant, a middle layer and the upper end of a red blood cell layer for 0.5-1 mm after centrifugation, and performing secondary centrifugation; (3) secondary centrifugation: performing secondary centrifugation at the centrifugation parameter of 2800 r / min for 10 min; sucking a supernatant liquid after centrifugation until 5 ml of the lower layer is left, which is the platelet-rich factor; and (4) uniformly mixing the platelet-rich factor with a calcium chloride-bovine thrombin solution in the volume ratio being 1:(8-10), leaving the mixture to stand for 2 min or longer to obtain the platelet-rich gelator. The allograft-derived platelet-rich gelator prepared by the method can be used for allograft therapy, has concentration up to 9 times that of original whole blood, can be used for acute and chronic wound repair and can be used as compound artificial bone for treatment of nonunion.

The invention discloses 1, 7-diphenyl-4-heptene-3-ketone separated from galangal and an application thereof. The separation method comprises the following steps that 42 kg of galangal is smashed, 80%ethyl alcohol is heated and refluxed three times, supernatant liquid and subnatant liquid are taken respectively to be dripped on a plate, and extraction is completed if the subnatant liquid is not dripped on the plate and the subnatant liquid drips out slightly; after the extraction is finished, the supernatant is taken, combining is carried out, and rotary evaporation and volatilizing are carried out to obtain a galangal extract; the extract is loaded on a column according to a ratio of 1: 7, 385g of silica gel and 55g of a silica gel protection column are carried out, fractions obtained bywashing petroleum ether, ethyl acetate, methanol and water according to eluent ratios of (30: 1), (25: 1), (20: 1), (15: 1), (10: 1), (5: 1) and (5: 3) are respectively connected, and a liquid phase is prepared. The galangal extract has a remarkable IR improving effect, and the compound can up-regulate the transcription level of Nrf2 mRNA and downstream target genes.

The invention relates to the field of tumor DNA detection, and particularly discloses a method for optimizing ctDNA detection accuracy, which comprises the following steps: S1, blood sample treatment: carrying out the following operations on a blood sample within 2 hours of blood sampling: centrifuging the blood sample, and taking upper-layer plasma; s2, extraction of ctDNA: adding a lysate, a protein enzymolysis agent and silicon dioxide into the plasma, uniformly mixing, centrifuging, taking a lower-layer precipitate, adding an EB buffer solution, carrying out water bath, taking out, centrifuging at room temperature, taking a supernatant as a ctDNA sample, and storing; s3, intermediate treatment of the ctDNA sample: heating the ctDNA sample to 1-10 DEG C, and carrying out ultrasonic treatment; and S4, ctDNA content detection: carrying out PCR amplification on the ctDNA sample, mixing the amplified ctDNA with a biosensor, carrying out a hybridization reaction, and detecting the fluorescence intensity. The ctDNA detection method has the advantages of high plasma extraction rate, short extraction time, high protein hydrolysis degree, high accuracy and high sensitivity.

The invention provides a method for jointly extracting multiple kinds of yolk protein based on water phase separation and a product. The method includes the steps that (1), fresh yolk is taken, a water phase solution with the volume 0.5-5 times that of the yolk is added for dilution, and the material is stirred, and is subjected to standing or is centrifugally layered; an upper-layer component A,a middle-layer component B and a lower-layer component C are obtained, wherein the water phase solution is polysaccharide matter with the mass percentage concentration of 0.1-0.5% or 2-8% of a water solution of low-molecular polymers; (2), the component A is taken, and yolk low-density lipoprotein is prepared; (3), the component B is taken, and yolk immune globulin is prepared; (4), the componentC is taken, and phosvitin and yolk high-density lipoprotein are prepared. The process is simple and short, the operation is easy, four kinds of yolk protein can be jointly extracted, the extracting efficiency is high, and the unit raw material output is high; extraction is carried out based on water phase separation, reagents used in the method are all food-grade or medicine-grade components, andthe reagents are green and safe; the treatment process is mild, and the functional characteristics and the bio-activity of the protein can be reserved to the maximum degree.

The invention discloses a novel lymphocyte separation and extraction device for blood examination, and belongs to the field of medical instruments. The novel lymphocyte separation and extraction device for blood examination comprises a base, wherein a first motor is fixedly mounted in the base, an output shaft of the first motor is fixedly connected with a rotating rod through a coupling, a rotating block is rotationally connected to the top of the base, the top end of the rotating rod penetrates through and is rotationally connected to the top of the base, the top end of the rotating rod is fixedly connected to the bottom of the rotating block, a reagent tube is inserted into the rotating block, a clamping assembly is arranged in the rotating block, and a sealing plug is arranged at the top of the reagent tube. According to the device, the situation that a blood sample is mixed with separation liquid before separation can be reduced, so that the separation effect is guaranteed, meanwhile, lymphocytes can be rapidly extracted in a layered mode, and the situations that supernatant is not completely extracted, lower-layer precipitates are extracted by mistake and the like are reduced.

The invention discloses a liquid mass spectrometry detection method for a variety of marine biological toxins in aquatic products. The samples of the aquatic products to be tested are extracted with 0.1vol% formic acid aqueous solution and acetonitrile in multiple stages, and the upper organic phase and the lower aqueous phase are obtained by layering. ; The lower aqueous phase was added to the MCX-HLB series column, rinsed with low-proportion methanol water, and then eluted once with the organic phase purified by dSPE, and then eluted with basic methanol for the second time. Liquid chromatography-tandem mass spectrometry is used for detection, and the external standard method is used for quantification to obtain the content of each component to be tested in the aquatic product sample to be tested. The invention can detect 8 kinds of marine biotoxins including hydrophilic and lipophilic at one time, and for the first time realizes one-step liquid mass spectrometry high-throughput quantitative detection of hydrophilic and lipophilic marine biotoxins.

The invention relates to a hemoglobin storage method which is characterized by comprising the following steps of adding a proper quantity of human blood into normal saline (a 0.9% NaCl solution) with the volume being 3-6 times that of the human blood, centrifuging at the temperature of 4 DEG C and the rotating speed of 2800r / min for 15-30 minutes to obtain a supernatant containing leukocytes, plasma proteins and platelets, extruding the supernatant, retaining deposited erythrocytes at the lower layer, repeating the washing process for more than 3-5 times until the supernatant is basically colorless, and removing other substances except for the erythrocytes to the maximum extent to obtain clean deposited erythrocytes; adding a certain quantity of deposited erythrocytes into equivoluminal sterilized high-purity deionized water to pre-expand for 5-15 minutes, finally, adding water with the volume being 2 times that of the deposited erythrocytes to carry out low permeation for 10-20 minutes, centrifuging at the rotating speed of 10000r / min for 30-60 minutes, and extracting the supernatant after centrifuging to obtain a hemoglobin solution; and subpackaging the purified hemoglobin solution into a 10ml sample bottle, sealing by using paraffin, and storing the purified hemoglobin solution in a brown bottle filled with a pyrogallic acid solution at the temperature of 4 DEG C, wherein the content of hemoglobin does not exceed 3% after the sample is stored for 4 months and is kept on the relatively-low level.

The invention discloses a centrifugal device for separating and preparing a biological sample, and belongs to the technical field of medical instruments or equipment. The centrifugal device for separating and preparing the biological sample comprises a rotary drum, a test tube, an autorotation module and a revolution module, the tail end of the test tube is flexible and controllable in opening and closing, the test tube is provided with a to-be-separated sample liquid , the tail of the test tube is inserted into a placement hole corresponding to the inner wall of the rotary drum and inclined towards the axis side, the autorotation module controls the test tube to rotate around an axis of the test tube, the tail end is opened, the revolution module controls the test tube to rotate around an axis of the rotary drum, after the test tube rotates, the tail end is opened, the sample liquid integrally descends, the sample liquid is layered after revolution centrifugation, the tail end is closed after rotation is stopped, lower-layer waste residues and upper-layer clear liquid are isolated, and after the test tube is taken out, the clear liquid can be directly used without re-extraction, so that the time is saved, and the efficiency is improved.

The invention discloses a pretreatment method for rapid detection of nitrofuran metabolites and a detection method thereof. The method comprises the following steps: 1) sequentially adding an acid solution and a derivative reagent to the crushed animal tissue to obtain a solution A; 2) heating the solution A and adding an extractant for extraction to obtain a supernatant; 3) adding an extraction agent to the supernatant An organic solvent with a polarity ranging from 0 to 3.5 and a buffer solution are sequentially added into the liquid, and the detection liquid of the lower layer is taken for detection. After adding the extractant for extraction, the present invention can not only effectively remove ethyl acetate, but also remove excess oil in the sample, so that the substance to be detected is directly dissolved in the buffer solution, which is simple and efficient , without the need for additional instruments, greatly simplifies the operation steps, saves the cost of instruments, improves the operability of on-site detection, and also significantly shortens the pretreatment time, realizing the rapid on-site detection of nitrofuran metabolites.

The invention belongs to the field of feed detection, and relates to a detection method of diludine in feed. The detection method comprises the following steps: weighing a proper amount of crushed and uniformly mixed feed sample, adding 0.1 mol / L of hydrogen peroxide solution, and standing in a thermostatic water bath; adding acetonitrile, homogenizing by a homogenizer and then centrifuging, evaporating supernatant by a rotary evaporator and then concentrating until the supernatant is nearly dry; fixing the volume with acetonitrile and degreasing with normal hexane, filtering a solution on a lower layer, and measuring by a high performance liquid chromatography-tandem mass spectrometer; carrying out qualitative and quantitative analysis by using qualitative and quantitative ions, wherein a detection limit is 0.001mg / kg. The detection method disclosed by the invention has the characteristics of accurate qualitative analysis, high quantification speed, simple steps, capability of effectively eliminating interference and the like; in addition, the detection method is suitable for detecting the addition amount of the diludine in livestock, poultry and aquatic feed being in a solid state at normal temperature.

The invention belongs to the technical field of natural concentrated growth factor CGF preparation, and relates to a method for preparing a solid or liquid natural concentrated growth factor CGF from autoblood. The method comprises the following steps: collecting blood, respectively injecting the blood into a purple sterile anti-coagulation test tube and a sterile vacuum red blood collection tube, adding a mixed anti-coagulation agent into the purple sterile anti-coagulation test tube, uniformly shaking, centrifuging, and taking a middle CGF layer to obtain liquid CGF; centrifuging the sterile vacuum red blood collection tube, taking the middle layer and the gel at the junction of the middle layer and the lower layer, and pressing and shaping by a film pressing device to obtain the solid CGF. The CGF preparation amount is large, the operation is convenient, the practicability is high, and the market application prospect is relatively high.

Provided herein are compositions, systems, and methods for extracting and detecting at least one HDL-associated protein (e.g., ApoA1) from a sample (e.g., plasma or serum sample). In certain embodiments, a strong organic acid and hydrophilic organic solvent are mixed with the sample; after centrifugation, the supernatant is transferred to a second container where it is mixed with a non-polar organic solvent; after centrifugation, the lower aqueous layer is transferred to a third container; and then at least a portion of the transferred aqueous layer is subjected to a detection assay such that at least one HDL-associated protein is detected.

The invention discloses a method for determining melatonin in faeces by using high performance liquid chromatography. The method comprises the following steps: mashing and uniformly mixing to-be-determined faeces, freeze-drying the to-be-determined faeces by using a freeze dryer, leaching the to-be-determined faeces by using methanol, centrifuging, taking a supernatant, and blow-drying the supernatant by using a termovap sample concentrator; performing dissolution by using an acetonitrile n-hexane solution, centrifuging, taking a subnatant, and filtering the subnatant by using a microfiltration membrane; and performing high performance liquid chromatography detection on a machine, and quantifying the melatonin in the faeces sample by adopting an external standard method. The melatonin in the faeces is extracted and detected for the first time; the detection method is high in sensitivity, high in specificity, good in stability and accurate and reliable in result; a novel animal non-invasive melatonin detection method can be established; and the circadian rhythm of animal endogenous melatonin secretion is clarified.

The invention discloses a method for detecting lactic acid bacteria in probiotics, and belongs to the technical field of detection of microbial products. The method includes: (1) adding the sample to be tested into PBS buffer solution added with BSAV, homogenizing to prepare a sample homogeneous solution, the dosage ratio of the sample to be tested and PBS buffer solution is 1g:9mL; Lactose solution was added to the solution, and the supernatant was removed by water bath and centrifugation to obtain a bacterial suspension; (2) Lactic acid bacteria immune magnetic beads were added to the bacterial suspension obtained in step (1), mixed and oscillated, and statically placed on a magnetic stand Set the layers, discard the upper layer, and elute the lower layer samples, add PBS buffer solution to the eluted lactic acid bacteria immunomagnetic beads, oscillate and resuspend the magnetic beads, and obtain the sample solution to be tested; (3) incubate the sample to be tested , observation, detection. The invention shortens the detection time of the lactic acid bacteria in the sample, improves the detection rate of the lactic acid bacteria and the accuracy of the detection results, and has high detection sensitivity.

The invention relates to the technical field of biology, in particular to a method for extracting nannochloropsis oculata EPA polar lipid with assistance of enzyme treatment. The method comprises the following steps: 1, treating nannochloropsis oculata by using laccase to obtain nannochloropsis oculata enzymolysis suspension; 2, centrifuging the nannochloropsis oculata enzymolysis suspension, removing supernatant liquid, taking lower precipitate, and performing drying to obtain nannochloropsis oculata enzymolysis wall-broken algae powder; 3, carrying out subcritical extraction on the wall-broken nannochloropsis oculata powder to obtain a nannochloropsis oculata polar lipid extracting solution; 4, centrifuging the nannochloropsis oculata polar lipid extracting solution, taking supernatant liquid, and removing the solvent through vacuum evaporation to obtain the nannochloropsis oculata EPA polar lipid. The preparation process of the nannochloropsis oculata EPA polar lipid is safe, the method is convenient, and the extraction rate of the EPA polar lipid is remarkably improved compared with the prior art.

The invention relates to a pretreatment method for determination of quinolone residues in animal-derived products, which comprises the following steps: weighing a proper amount of a sample, putting the sample into a centrifuge tube, adding a formic acid-acetonitrile solution, and homogenizing with a homogenizer; after homogenizing, adding anhydrous sodium sulfate into the centrifuge tube, and uniformly mixing; centrifuging, and extracting supernatant for the second time; adding acetonitrile saturated n-hexane into the supernatant, shaking, discarding the upper-layer solution, and transferringthe lower-layer extracting solution into a heart-shaped bottle; adding n-propanol or isopropanol into a heart-shaped bottle, and then carrying out rotary evaporation; adding a formic acid-acetonitrilesolution to dissolve residues, filtering, fixing the volume, transferring the fixed solution into a 10mL centrifuge tube after fixing the volume, adding acetonitrile saturated n-hexane, performing vortex mixing, performing low-temperature high-speed centrifugation, taking the lower-layer liquid, and detecting on an organic filter membrane machine. According to the method, the phenomenon that thesolution in the heart-shaped bottle is exploded and boiled during rotary evaporation is reduced, the problem that the nitrogen blowing time is too long is solved, and the phenomenon that white particles are separated out of the high-fat sample final solution is avoided.

The invention discloses a device and a method for recovering a chromatographic grade organic reagent from an organic reagent waste liquid. The device comprises an organic reagent waste liquid salting-out tank, an organic reagent distillation kettle, an organic reagent pervaporation membrane separation device and a saturated salt solution distillation tank. The method comprises the following steps: the organic reagent waste liquid enters the organic reagent waste liquid salting-out tank; after the organic reagent waste liquid is added into the organic reagent waste liquid salting-out tank from the saturated salt solution distillation tank according to a certain proportion, carrying out sufficient stirring and standing; after stratification, a supernviaould like liquid returns to the saturated salt solution distillation tank for distillation; after the distillation, a saturated salt solution is obtained for repeated use; a supernatant organic solvent liquid is transferred to the organic reagent distillation kettle. Through the adoption of the device, a chromatographic grade organic reagent waste liquid can be collected; the chromatographic grade organic reagent, namely an effective constituent in the waste liquid, can be recovered by certain means, such as the coupling between salting-out and dewatering, the coupling between distillation and impurity removing, and the coupling between pervaporation and liquid-liquid separation; the recovered chromatographic grade organic reagent can reach the national standard.

The invention discloses a kit for lipoprotein determination. The kit comprises a determination agent placement layer, a medicament placement layer and a box; the medicament placement layer comprises amedicament placement tube, a rubber base, a bottom layer chilling plate, a drawer and a support plate; and the medicament placement layer comprises a determination placement tube, a determination support rack, a determination bottom rack, a top fixing plate, a ventilation opening, an upper layer chilling plate, an electric fan and a humidifier. According to the kit for the lipoprotein determination provided by the invention, an upper layer and a lower layer are placed separately, an agent to be tested is placed on the upper layer, and a medicament needed for the test is placed on the lower layer, and the storage methods of the agent to be tested and the medicament are inconsistent, so that the upper and lower layers are separated, thus the agent to be tested can be tested during the placement process, thereby saving time and improving the efficiency.

The invention discloses a preparation method and application of magnetic nanoparticles for capturing exosomes in blood, which is characterized by the following steps: (1) mixing ferrous chloride hexahydrate and ferrous chloride tetrahydrate In distilled water, stir and mix well and heat to 70°C, then slowly add ammonia solution dropwise, after the hydrolysis is basically completed, slowly add cetyltrimethylamine bromide to the solution, stir and centrifuge to collect the product for washing, take the lower precipitate and dry it in vacuum to obtain Nano Fe 3 o 4 Particles; (2) take nano Fe 3 o 4 After the particles were dispersed in an aqueous ethanol solution and ultrasonically treated, after adding tetraethyl orthosilicate solution, they were gradually added to an aqueous ammonia water bath to react for 5 h, and the product was collected by centrifugation, washed and dried to obtain SiO coated particles. 2 In addition, irregular magnetic micro-nano particles with a pore size of 40-200 nm are distributed on the surface, which has the advantages of binding exosomes without damage to the greatest extent, and the sample volume used is small, the processing time is short, and the operation is simple.

A preparation method of adiposomes, and use thereof. Provided is a method for preparing adiposomes consisting of neutral lipids and a monolayer phospholipid membrane, comprising a1) vortexing phospholipid and neutral lipids in a buffer, centrifuging the resulting mixture, and collecting an upper liquid phase; a2) purifying the upper liquid phase twice or more by uniformly mixing the upper liquid phase with the buffer, layering the mixture, and collecting an upper liquid phase; and a3) uniformly mixing the upper liquid phase obtained in step a2) with the buffer, layering the mixture, and collecting a lower liquid phase in containing adiposomes. For the adiposomes prepared by the method, one or more resident proteins and / or functional proteins can be recruited to obtain artificial lipid droplets, and one or more apolipoproteins can be recruited to obtain artificial lipoproteins; and they all play important roles in preparing drugs and / or drug carriers.

The invention belongs to the technical field of poultry product detection and relates to a combined detection method of amantadine, rimantadine, ribavirin and moroxydine residues in eggs. The method comprises the following steps: carrying out low temperature repetitive freeze-thawing on a sample, adding a formic acid-methanol solution, mixing, centrifuging, taking a supernatant for standby, adding water saturated n-hexane, mixing, carrying out ultrasonic treatment, centrifuging to remove n-hexane floccules on the upper layer with an extracting solution left at the lower layer, purifying with a cation exchange solid-phase extraction column, measuring by a liquid chromatography-tandem mass spectrometer provided with an ESI source, and carrying out accurate qualitative and quantitative analysis on residues of the four antiviral drugs in eggs. The method provided by the invention has high specificity, can accurately and simultaneously measure residual quantity of amantadine, rimantadine, ribavirin and moroxydine without pollution, has high sensitivity, and provides technical support for guaranteeing quality safety of eggs.

The invention discloses a device and a method for preparing and collecting lipidosome. The device comprises a signal generator and a preparation and collection chip, wherein the preparation and collection chip comprises a preparation chamber, a collection chamber and a waste fluid chamber; the preparation chamber comprises an electrode couple and a preparation cavity; the electrode couple is connected with the signal generator through electric conduction tapes; the collection chamber comprises an upper-layer collection cavity and a lower-layer collection cavity; a layer of microfiltration membrane is arranged between the two cavities; and the preparation chamber, the collection chamber and the waste fluid chamber are connected through microchannels. The device for preparing and collecting lipidosome mainly uses the method of powering up to prepare the lipidosome, and utilizes the microfiltration membrane to collect and process the lipidosome on the same chip. Compared with the prior art, the device and the method have the following technical effects: the large blank space lipidosome and the large lipidosome carried with drug, protein and particles can be effectively prepared; the preparation and collection of the lipidosome are integrated to reduce pilot process; and the required dose of reagents is less.