37 results about "Infranatant" patented technology

The invention discloses a liquid mass spectrometry detection method for a variety of marine biological toxins in aquatic products. The samples of the aquatic products to be tested are extracted with 0.1vol% formic acid aqueous solution and acetonitrile in multiple stages, and the upper organic phase and the lower aqueous phase are obtained by layering. ; The lower aqueous phase was added to the MCX-HLB series column, rinsed with low-proportion methanol water, and then eluted once with the organic phase purified by dSPE, and then eluted with basic methanol for the second time. Liquid chromatography-tandem mass spectrometry is used for detection, and the external standard method is used for quantification to obtain the content of each component to be tested in the aquatic product sample to be tested. The invention can detect 8 kinds of marine biotoxins including hydrophilic and lipophilic at one time, and for the first time realizes one-step liquid mass spectrometry high-throughput quantitative detection of hydrophilic and lipophilic marine biotoxins.

The invention discloses a pretreatment method for rapid detection of nitrofuran metabolites and a detection method thereof. The method comprises the following steps: 1) sequentially adding an acid solution and a derivative reagent to the crushed animal tissue to obtain a solution A; 2) heating the solution A and adding an extractant for extraction to obtain a supernatant; 3) adding an extraction agent to the supernatant An organic solvent with a polarity ranging from 0 to 3.5 and a buffer solution are sequentially added into the liquid, and the detection liquid of the lower layer is taken for detection. After adding the extractant for extraction, the present invention can not only effectively remove ethyl acetate, but also remove excess oil in the sample, so that the substance to be detected is directly dissolved in the buffer solution, which is simple and efficient , without the need for additional instruments, greatly simplifies the operation steps, saves the cost of instruments, improves the operability of on-site detection, and also significantly shortens the pretreatment time, realizing the rapid on-site detection of nitrofuran metabolites.

The invention belongs to the field of feed detection, and relates to a detection method of diludine in feed. The detection method comprises the following steps: weighing a proper amount of crushed and uniformly mixed feed sample, adding 0.1 mol / L of hydrogen peroxide solution, and standing in a thermostatic water bath; adding acetonitrile, homogenizing by a homogenizer and then centrifuging, evaporating supernatant by a rotary evaporator and then concentrating until the supernatant is nearly dry; fixing the volume with acetonitrile and degreasing with normal hexane, filtering a solution on a lower layer, and measuring by a high performance liquid chromatography-tandem mass spectrometer; carrying out qualitative and quantitative analysis by using qualitative and quantitative ions, wherein a detection limit is 0.001mg / kg. The detection method disclosed by the invention has the characteristics of accurate qualitative analysis, high quantification speed, simple steps, capability of effectively eliminating interference and the like; in addition, the detection method is suitable for detecting the addition amount of the diludine in livestock, poultry and aquatic feed being in a solid state at normal temperature.

The invention discloses a method for detecting lactic acid bacteria in probiotics, and belongs to the technical field of detection of microbial products. The method includes: (1) adding the sample to be tested into PBS buffer solution added with BSAV, homogenizing to prepare a sample homogeneous solution, the dosage ratio of the sample to be tested and PBS buffer solution is 1g:9mL; Lactose solution was added to the solution, and the supernatant was removed by water bath and centrifugation to obtain a bacterial suspension; (2) Lactic acid bacteria immune magnetic beads were added to the bacterial suspension obtained in step (1), mixed and oscillated, and statically placed on a magnetic stand Set the layers, discard the upper layer, and elute the lower layer samples, add PBS buffer solution to the eluted lactic acid bacteria immunomagnetic beads, oscillate and resuspend the magnetic beads, and obtain the sample solution to be tested; (3) incubate the sample to be tested , observation, detection. The invention shortens the detection time of the lactic acid bacteria in the sample, improves the detection rate of the lactic acid bacteria and the accuracy of the detection results, and has high detection sensitivity.

The invention relates to a reagent for removing lipids in hyperlipidemia serum and an application of the reagent to an apolipoprotein E detection process. A method comprises the steps of mixing the serum with the reagent for removing the blood lipids; and stirring and uniformly mixing the serum and the reagent, and carrying out standing for 10 minutes, so that the serum is layered, wherein the lower layer is blood-lipid-free serum, and the upper layer is a white separated blood lipid part.

The invention discloses a device and a method for recovering a chromatographic grade organic reagent from an organic reagent waste liquid. The device comprises an organic reagent waste liquid salting-out tank, an organic reagent distillation kettle, an organic reagent pervaporation membrane separation device and a saturated salt solution distillation tank. The method comprises the following steps: the organic reagent waste liquid enters the organic reagent waste liquid salting-out tank; after the organic reagent waste liquid is added into the organic reagent waste liquid salting-out tank from the saturated salt solution distillation tank according to a certain proportion, carrying out sufficient stirring and standing; after stratification, a supernviaould like liquid returns to the saturated salt solution distillation tank for distillation; after the distillation, a saturated salt solution is obtained for repeated use; a supernatant organic solvent liquid is transferred to the organic reagent distillation kettle. Through the adoption of the device, a chromatographic grade organic reagent waste liquid can be collected; the chromatographic grade organic reagent, namely an effective constituent in the waste liquid, can be recovered by certain means, such as the coupling between salting-out and dewatering, the coupling between distillation and impurity removing, and the coupling between pervaporation and liquid-liquid separation; the recovered chromatographic grade organic reagent can reach the national standard.

The invention discloses a preparation method and application of magnetic nanoparticles for capturing exosomes in blood, which is characterized by the following steps: (1) mixing ferrous chloride hexahydrate and ferrous chloride tetrahydrate In distilled water, stir and mix well and heat to 70°C, then slowly add ammonia solution dropwise, after the hydrolysis is basically completed, slowly add cetyltrimethylamine bromide to the solution, stir and centrifuge to collect the product for washing, take the lower precipitate and dry it in vacuum to obtain Nano Fe 3 o 4 Particles; (2) take nano Fe 3 o 4 After the particles were dispersed in an aqueous ethanol solution and ultrasonically treated, after adding tetraethyl orthosilicate solution, they were gradually added to an aqueous ammonia water bath to react for 5 h, and the product was collected by centrifugation, washed and dried to obtain SiO coated particles. 2 In addition, irregular magnetic micro-nano particles with a pore size of 40-200 nm are distributed on the surface, which has the advantages of binding exosomes without damage to the greatest extent, and the sample volume used is small, the processing time is short, and the operation is simple.

A preparation method of adiposomes, and use thereof. Provided is a method for preparing adiposomes consisting of neutral lipids and a monolayer phospholipid membrane, comprising a1) vortexing phospholipid and neutral lipids in a buffer, centrifuging the resulting mixture, and collecting an upper liquid phase; a2) purifying the upper liquid phase twice or more by uniformly mixing the upper liquid phase with the buffer, layering the mixture, and collecting an upper liquid phase; and a3) uniformly mixing the upper liquid phase obtained in step a2) with the buffer, layering the mixture, and collecting a lower liquid phase in containing adiposomes. For the adiposomes prepared by the method, one or more resident proteins and / or functional proteins can be recruited to obtain artificial lipid droplets, and one or more apolipoproteins can be recruited to obtain artificial lipoproteins; and they all play important roles in preparing drugs and / or drug carriers.

The invention belongs to the technical field of poultry product detection and relates to a combined detection method of amantadine, rimantadine, ribavirin and moroxydine residues in eggs. The method comprises the following steps: carrying out low temperature repetitive freeze-thawing on a sample, adding a formic acid-methanol solution, mixing, centrifuging, taking a supernatant for standby, adding water saturated n-hexane, mixing, carrying out ultrasonic treatment, centrifuging to remove n-hexane floccules on the upper layer with an extracting solution left at the lower layer, purifying with a cation exchange solid-phase extraction column, measuring by a liquid chromatography-tandem mass spectrometer provided with an ESI source, and carrying out accurate qualitative and quantitative analysis on residues of the four antiviral drugs in eggs. The method provided by the invention has high specificity, can accurately and simultaneously measure residual quantity of amantadine, rimantadine, ribavirin and moroxydine without pollution, has high sensitivity, and provides technical support for guaranteeing quality safety of eggs.