6 results about "Luminescent Proteins" patented technology

The application relates to the technical field of bioengineering, in particular to a preparation method of a pre-dyed fluorescent protein Marker, which comprises the following steps: preparing a subunit mixture: 1 mg of luminescent protein precipitate is placed in a centrifuge tube, high-speed centrifugation is carried out at 4 DEG C for 5 min, supernatant and precipitate are obtained, the supernatant is completely sucked away by using a pipette gun, and only the precipitate part is reserved. The fluorescent protein Marker is a naturally extracted fluorescent protein, the molecular weight of the fluorescent protein is 20-300 kDa, the fluorescent protein is covalently coupled with protein A and protein G which can be combined with an antibody IgG and an antibody Fc region protein or peptide which can be combined with a secondary antibody, due to the color of the fluorescent protein, the purpose that the protein is pre-dyed protein and fluorescent protein is achieved, transfer film staining or X-ray film imaging is not needed, electrophoresis is simpler, due to the very strong fluorescent signal, even if a trace amount of protein, electrophoresis detection can be realized, and an ideal electrophoresis map is obtained.

This disclosure relates to compositions containing apomylated jellyfish luminescent protein and methods for treating neuronal inflammation. The invention relates to a method of premodulating neurons to reduce neuronal inflammation in a subject. The method includes the step of administering apomylated jellyfish luminescent protein to the subject, wherein the subject's neurons are premodulated to reduce subsequent neuronal inflammation in the subject.

The invention relates to the technical field of biomechanical luminescence diagnosis and treatment, in particular to a fusion protein and a reagent for ultrasonically regulating and controlling cell luminescence. A force response bioluminescent protein system composed of mechanical force sensitive ion channel (Msc) protein and jellyfish luminescent protein (AEQ) is constructed in cells, and transmembrane conduction of Ca < 2 + > under the action of ultrasonic mechanical force is achieved. And Ca < 2 + > is specifically combined with jellyfish luminescent protein, and the oxidized coelenterazine substrate emits light, so that the bioluminescence process regulated and controlled by ultrasound is achieved. The Ca < 2 + >-sensitive jellyfish luminescent protein is used as an ultrasonic mechanical force response bioluminescent carrier, and can generate instantaneous luminescence under ultrasonic control, so that the ultrasonic has the switch function characteristic of controlling the luminescence of the protein. Meanwhile, based on the non-invasiveness and excellent tissue penetrating capacity of ultrasound, accurate space-time positioning in the body can be achieved. Therefore, bioprotein luminescence triggered by ultrasound is not limited by treatment depth, and the technical bottleneck of in-vivo application of light therapy is broken through.

This disclosure describes novel methods for imaging or evaluating partial oxygen tension in a tissue in vitro or in vivo in a subject as well as methods for detecting or evaluating a level of oxygen in a target. A method of identifying an agent capable of modulating partial oxygen tension in a tissue of a subject and a method of evaluating an effect of an agent on partial oxygen tension in a tissue of a subject are also disclosed. The methods involve introducing or expressing in the tissue or in one or more cells therein a luminescent protein capable of oxidizing a substrate with oxygen in the tissue, wherein oxidization of the substrate by the luminescent protein results in emission of a bioluminescence signal, and wherein intensity of the bioluminescence signal is correlated to a level of oxygen in the tissue; contacting the tissue with the substrate under a condition permitting oxidization of the substrate by the luminescent protein and emission of the bioluminescence signal; and imaging or evaluating the partial oxygen tension in the tissue by detecting the bioluminescence signal or determining a level of the bioluminescence signal. Preferably the luminescent protein is a fusion protein comprising a luciferase fused to a fluorescent protein.

The present disclosure, in one aspect, provides a modified luciferase with improved luminescence intensity, and use thereof. The present disclosure relates to a modified luciferase derived from a luminescent mushroom or a functional fragment thereof, including a mutation introduced into arginine or lysine corresponding to position 26 and / or a mutation introduced into valine corresponding to position 161 of amino acid sequence shown in SEQ ID NO: 1, and having enhanced luminescence compared with a luciferase before the mutation is introduced. The present disclosure also relates to an isolated nucleic acid encoding the modified luciferase or functional fragment thereof of the present disclosure.

The invention discloses a protein and rare earth complex co-crystallized Cu < 2 + > fluorescence sensor and a method and application thereof, and the method comprises the following steps: blending a rare earth complex solution and a protein solution, carrying out a product precipitation co-crystallization method to obtain a rare earth complex doped luminescent protein crystal, carrying out annealing treatment on the crystal, and carrying out chemical crosslinking to obtain the Cu < 2 + > fluorescence sensor. And a crystallization structure with enhanced stability is obtained. The protein crystal can be used as a carrier with good biocompatibility, the hybrid luminous crystal is obtained by co-crystallizing the protein crystal and a rare earth complex, the hybrid luminous crystal can effectively detect the concentration of Cu < 2 + > in blood or a solution, and the luminous intensity of the crystal is changed along with the change of the ion concentration.