The invention relates to the technical field of nuclear reactor thermal hydraulic design and safety analysis, and particularly discloses a CHF relational expression DNBR limit value statistical determination method based on a grouping method. The method comprises the following steps: 1, acquiring CHF experimental data of a fuel assembly; 2, obtaining M/P data of an experiment burning point position; 3, carrying out a Bartlett test; 4, after passing the Bartlett test, carrying out homogeneity test of a data mean value; 5, carrying out normal distribution inspection by adopting an Epps-Pulley inspection method; 6, determining a DNBR limit value by using an Own criterion; 7, performing W-M-W inspection and K-W unilateral variance analysis respectively; 8, determining a unilateral 95/95 limitvalue of free distribution, and obtaining a DNBR limit value; 9, after the Bartlett test is not passed, carrying out a homogeneity test of a data mean value; 10, performing data grouping inspection; 11, determining a DNBR limit value. According to the method, a rigorous, accurate and relatively conservative CHF relational expression DNBR limit value can be obtained, key parameters can be calculated for CHF relational expression development and CHF experimental data evaluation, and the most concerned design limit value is provided for a nuclear safety department.