67 results about "Oxyntic cell" patented technology

The present invention is related to oral compositions comprising an irreversible gastric H+ / K+-ATPase proton pump inhibitor (PPI) as a gastric acid secretion inhibitor and succinc acid as a parietal cell activator in the gastric lumen. The compositions of the present invention are capable of enhancing the anti-acid activity of PPI in the stomach. The present invention further relates to a method of using such compositions to reduce gastric acid secretion in a mammal.

The invention relates to methods for identifying agents, which induce oxidative cell death in a tumor cell. The invention further relates to methods for identifying tumor cells, which are sensitive to agents that induce oxidative cell death. The invention also relates to methods for identifying subjects who are suffering from tumors, which are sensitive to agents that induce oxidative cell death.

The invention provides a stem cell culture medium and a method for culturing endometrium stem cells by using the stem cell culture medium. The method comprises the following steps: separately collecting menstrual blood and endometrium tissues, respectively culturing the menstrual blood and endometrium tissues in the stem cell culture medium provided by the invention to respectively obtain menstrual blood adherent cells and endometrium adherent cells, culturing the menstrual blood adherent cells and endometrium adherent cells in a cell culture bottle, collecting the adherent cells by trypsinization, inoculating the adherent cells in a cell coculture dish, and culturing the adherent cells in the stem cell culture medium provided by the invention. The stem cell culture medium has the advantages of simple components, fewer added components and lower cost. After more than 20 generations of in-vitro culture, the cells can not easily have the phenomenon of aging or degeneration, and can maintain the activity and stem property of the stem cells for a long time. The stem cell culture method is simple and effective, the cell proliferation efficiency is high, and the in-vitro culture doubling time is only 20 hours or so. The cells can be stably amplified by 50 generations.

The invention discloses a method of preparing microfibrillated cellulose from bamboo parenchyma cells, which comprises following steps: (1) separation of the bamboo parenchyma cells: performing chemical separation or drying and crushing process to raw materials, sieving the treated raw materials through a 20-mesh sieve screen to obtain the parenchyma cells; (2) chemical pre-treatment: adding the parenchyma cells to a phenethyl alcohol solution for extraction, adding a 1-3% sodium chlorite solution for treating the parenchyma cells for one hour, and adding the parenchyma cells in an alkaline solution for treating the parenchyma cells for two hours, treating the parenchyma cells in a diluted hydrochloric acid solution for two hours, and finally washing the parenchyma cells with deionized water to neutralization; and (3) dispersing the treated parenchyma cells in deionized water to form a 1% suspension liquid, and performing high-frequency ultrasonic treatment or high-pressure homogenizing treatment. According to the method, the bamboo parenchyma cells are separated through the sieving process in early stage to prepare the microfibrillated cellulose. The raw materials are wide in sources and are low in cost. The method is simple and easy to carry out, is high in production efficiency, can reach 90% in yield and can basically achieve all-conversion of the raw materials.

The present invention provides a method for separating out rice root xylem parenchyma cell protoplasts. The method chooses the part of the extended and the mature sections of a rice Oryza sativa L. seeding root, which is 2cm to 3cm long and does not has lateral roots grown, uses hydrolytic enzyme liquids with different ingredients and finds the optimal separation conditions to successfully separate out the intact stele xylem parenchyma cell protoplasts. At present, the related report is not published yet at home and abroad.

The present invention is related to novel oral compositions comprising an irreversible gastric H+ / K+-ATPase proton pump inhibitor (PPI) as a gastric acid secretion inhibitor and one or more aliphatic carboxylic acid derivative molecules which activate parietal cells, wherein the derivatives possess delayed or sustained enhancement effect on the PPI activity compared to the non-derivatized acid molecules. The present invention further relates to a method of using such compositions to reduce gastric acid secretion in a mammal.

The invention relates to a laboratory adherent cell thin film culture device and the method of the field of biological engineering technology, and the device includes a flat dish, an organic polymer thin film layer and an adhesive liquid layer. The organic polymer thin film layer is adhered at the inner bottom surface of the flat dish. The polymer material thin film is wetted with the adhesive liquid to be adhered at the bottom surface of a culture dish as an adherent cell attachment surface, so as to facilitate the rapid separation of the cells during the passage, the cells are rapidly put into a volumetric culture liquid and then are exfoliated by swinging method, further to carry out counting and passage based on this, the accurate component ensures the parallel initial inoculation conditions of the next generation; compared with the original passage method, the operation is more simple, and the bacteria contamination opportunities are fewer. The different polymer thin films can be attached for matching different needs, so as to improve digestive efficiency or survival rate of the adherent culture.

The invention discloses a three-dimensional dyeing method for amygdalus communis l anther cells. According to the three-dimensional dyeing method, an amygdalus communis l anther slice treated with a conventional paraffin slicing method is dyed with an iron haematoxylin solution prepared 10 min in advance, such that the pollen granulocytes, the tapetal cells, the intermediate cells, the connective cells, the endothecium cells, the epidermic cells, the cells at the connection tissue position and the filaments of the amygdalus communis l anther can be clearly colored, and the coloring degrees among the cells at different parts are different so as to provide the three-dimensional dyeing effect. The three-dimensional dyeing method can be used for the study on the three-dimensional dyeing method internal structure change and the anther development process.

The invention discloses functions and application of responsive to centrifugal force and shear stress gene 1 (RECS1) to treatment of restenosis after vascular injury. An RECS1 knockout mouse and a wild type mouse are taken as experimental subjects, and detection on mouse internal membrane regeneration, vessel wall cell proliferation level and smooth muscle cell phenotype transformation is carried out by a vascular injury model. Detection results prove that by virtue of RECS1 knockout, internal membrane regeneration and cell proliferation can be obviously inhibited, and smooth muscle cells are inhibited to be transformed into synthetic phenotype from contractile phenotype. Therefore, the functions of the RECS1 to the restenosis after vascular injury are mainly embodied in that internal membrane regeneration, cell proliferation and smooth muscle cell phenotype transformation are promoted by the RECS1. Aiming at the functions of the RECS1, the RECS1 can be used as a drug target for screening medicines for preventing, relieving and / or treating the disease of restenosis after vascular injury; an inhibitor of the RECS1 can be used for preparing medicines and arterial stents for preventing, relieving and / or treating the restenosis after vascular injury.

The invention discloses the function of IRF 9 in stent and carotid endarterectomy restenosis as well as an application of an inhibitor of IRF9, belonging to the field of the function and the application of a gene. According to the invention, IRF9 gene knockout mice and wild C57 mice are taken as experimental subjects, detection of mouse intima neogenesis, vascular wall cell proliferation level and smooth muscle cell phenotype is performed through a vascular injury model, and the results show that IRF9 gene knockout mice represents inhibited intima neogenesis and cell proliferation in comparison with the wild C57 mice. The invention discloses the function of the IRF9 gene in the stent and carotid endarterectomy restenosis, which mainly shows that the IRF9 gene has the function of promoting the intima neogenesis and cell proliferation. According to the abovementioned function, the IRF9 can be used as a drug target for screening drugs for treating hemadostenosis diseases, and the inhibitor of the IRF9 can be used for preparing the drug for treating the stent and carotid endarterectomy restenosis.

The present invention relates to a culture method of hair follicle mesenchymal stem cells and belongs to the field of biology. The culture method of the hair follicle mesenchymal stem cells comprisesthe following steps: step 1, acquiring primary hair follicle tissue blocks; step 2, inoculating the obtained primary hair follicle tissue blocks into a cell culture medium for a culture treatment; andstep 3, carrying out biological identification on amplified cells, wherein results show that (1) the cells are adhered to walls; (2) cell phenotypes are as follows: CD44+ is greater than or equal to95%, CD105+ is greater than or equal to 95%, CD45+ is less than or equal to 2% and CD34+ is less than or equal to 2%; and (3) the cells can be differentiated into adipocytes, osteocytes and chondrocytes. The culture method can effectively improve proliferation of the hair follicle mesenchymal stem cells, improves an amplification capacity of the hair follicle mesenchymal stem cells in a culture process, is convenient to obtain materials, has small pain to obtain the materials, and is rich in sources and simple in separation.

The invention discloses functions and application of SHPS1 in treatment of post-vascular injury restenosis, belonging to the field of functions and applications of genes. SHPS1 gene knockout mice and wild type mice are taken as experimental subjects, and due to a vascular injury model, the mouse neointima formation, vascular wall cell proliferation level and smooth muscle cell phenotype transform are detected. The result proves that SHPS1 gene knockout can obviously promote neointima neogenesis and cell proliferation and promote transformation of smooth muscle cells from a shrinkage manner to a synthetic manner. Thus the functions of SHPS1 in post-vascular injury restenosis are realized, which mainly shows that SHPS1 has the functions of inhibiting neointima formation and cell proliferation and inhibiting smooth muscle cell phenotype transformation. Aiming at the functions of the SHPS1, the SHPS1 can be used for preparing medicines and arterial stents for preventing, relieving and / or treating the post-vascular injury restenosis.

The invention discloses a culture solution and a culture method of DC cells. The culture solution of the DC cells is added in batches according to the growth condition of the DC cells, namely an adherent cell culture solution, a subsequent first supplemented DC cell culture solution and a subsequent second supplemented DC cell culture solution, wherein the adherent cell culture solution comprises a serum-free DC culture medium, GM-SCF and IL-13; the subsequent first supplemented DC cell culture solution comprises a serum-free DC culture medium, GM-CSF, IL-4, an anti-CD 83 antibody and autoserum with the concentration of 1%; and the subsequent second supplemented DC cell culture solution comprises TNF-a, vitamin B12 and nicotinamide. By applying the culture solution and the culture method disclosed by the invention, the DC cells are cultured to be mature in a short time, the DC cells can be mature in only 3 days, the number and purity of the cells are high, the antigen presentation ability of the DC cells is strong, and the culture solution has an excellent anti-tumor effect.

The invention relates to the technical field of biology, in particular to an immune cell and a preparation method thereof. The preparation method comprises the steps that tumor cell nucleotide is mixed with supernate, and a first preparation is obtained; PBMC is taken for culture, and a suspension cell and an adherent cell are separated; the adherent cell is cultured through a culture medium containing the first preparation, and a DC cell is obtained; induced culture is conducted on the suspension cell, and an immune cell with killing efficiency is obtained; the DC cell and the immune cell with the killing efficiency are cultured together, and the immune cell is prepared. According to the the immune cell and the preparation method thereof, the tumor cell nucleotide and protein allergic sources are utilized simultaneously, strengthening stimulation is conducted on the DC cell, stronger antigen sensitization is exerted, higher affinity with a tumor cell is achieved, and the risk factor of the tumor cell does not exist.

The present invention is related to novel oral compositions comprising an irreversible gastric H+ / K+-ATPase proton pump inhibitor (PPI) as a gastric acid secretion inhibitor and one or more small carboxylic acid molecules as parietal cell activators in the gastric lumen. Unexpectedly, the compositions of the present invention are capable of enhancing the anti-acid activity of PPI in the stomach. The present invention further relates to a method of using such compositions to reduce gastric acid secretion in a mammal.

The invention relates to a method for extracting anchorage-dependent cells. The method comprises the following steps of preprocessing polyacrylamide hydrogel, culturing anchorage-dependent cells, integrally excavating the cells and the adhered gel, removing surface gel and the like. According to the method, an anchorage-dependent cell extracting device suitable for the extracting method is also arranged. The extracting method disclosed by the invention is convenient and easy to operate, and the extracting of the anchorage-dependent cells can be completed without tedious steps, so that the possibility of mistaking is reduced; besides, the adopted extracting device is simple in structure and low in cost; and in addition, the extracting method is small in damage to the anchorage-dependent cells, and has important significance to cell research.

The present invention provides a cell carrier containing a nanofiber composed of water-insoluble polysaccharides, preferably chitin or chitosan nanofiber; a carrier capable of being a common carrier in various operations such as i) suspension culture, ii) differentiation induction, iii) transportation and preservation under non-freezing conditions, iv) transplantation, v) recovery of bioactive substance from culture supernatant and the like of adherent cells; and continuous performance of plural operations selected from i) suspension culture, ii) differentiation induction, iii) transportation and preservation under non-freezing conditions, iv) transplantation, and v) recovery of bioactive substance from culture supernatant of adherent cells by using the carrier.

The invention discloses a suspension culture method of adherent cells and application thereof. The suspension culture method and the application provide modified adherent cells by high expression of transformation regulation medium matters related to cell suspension culture in the adherent cells; and then the modified adherent cells are cultured by the suspension culture method. The transformationregulation medium matters related to the cell suspension culture are subjected to higher level expression in the adherent cells, and the modified adherent cells are not prone to clustering in suspension culture, the improvement of cell survival rate is facilitated, the expression of foreign proteins or the preparation of virus vaccines is facilitated, and the production efficiency is improved. Therefore, the suspension culture method is particularly suitable for the production of recombinant proteins or the virus vaccines.

The invention provides a female-rat vaginal-smear sample collection device. The female-rat vaginal-smear sample collection device comprises a vagina built-in head and a normal saline pusher. The quantitative normal saline pusher is adopted, and the consistency of normal saline for sampling is guaranteed. In the vagina built-in head, a cervix end and an orificium vaginae end are fixed for guaranteeing the consistency of a sampling part; a soft material is used in the cervix end, and operational friction stimulation is reduced; meanwhile, the flow direction of the normal saline entering the vagina can be changed to be outwards, the cervix is protected, infection caused when the normal saline reversely flows is avoided, and infection caused when cells of the inner wall of the vagina are injured is avoided in the mode that once washing is carried out with saline. In the operation process, the normal saline can be kept in the aseptic condition, and the animal infection rate in the operation process is reduced; the vagina built-in head can be disinfected and washed to be reused.

The invention discloses a preparation method of a parenchymal cell cellulose and liquid metal nano-droplet composite membrane, which comprises the following steps: by taking agricultural waste bagasse pith as a source of parenchymal cell cellulose, removing lignin by using a chlorine dioxide method, and then removing hemicellulose by using an organic guanidine aqueous solution method to obtain parenchymal cell cellulose; the method comprises the following steps: dissolving parenchyma cell cellulose through DMAC and LiCl to obtain a parenchyma cell cellulose dissolving solution; the preparation method comprises the following steps: ultrasonically dispersing liquid metal into nano liquid drops by using isopropanol, modifying by using APTES to obtain SML NDs, and finally preparing the composite membrane by using a grafting reaction caused by N, N-methylene bisacrylamide and ammonium persulfate among cellulose macromolecules. The composite film has antibacterial ability and stretchability, also has good physical strength, can be completely degraded, has low toxicity and good skin fitness, and provides a feasible scheme for expanding the application field of bagasse pith and improving the additional value of bagasse pith.

The invention belongs to the technical field of biochemistry, and particularly relates to a digestion staining solution and a crystal violet staining cell nucleus counting method. In order to solve the problems that pancreatin digestive juice digestion time is not easy to control in a sheet-shaped carrier adherent culture mode, and digested cells cannot be completely separated from a net-shaped space in the prior art, the invention provides the digestive staining solution which is a mixed solution of crystal violet, citric acid and triton X100. The crystal violet staining cell nucleus countingmethod comprises the following steps of: (1) mixing a carrier carrying cells with the digestion staining solution, and staining and digesting the mixture to obtain a staining solution; and (2) carrying out cell nucleus counting on the staining solution. The invention further provides application of triton X100 in digesting adherent cells in a cell nucleus counting process. According to the crystal violet staining cell nucleus counting method, the phenomena of cell damage and even death caused by overlong time in the digestion process can be reduced, so that the accuracy of cell counting is improved.

The invention discloses a plant cell sap extraction method and device, and the extraction method comprises the following steps: S1) taking fresh plant fragments or a to-be-extracted product saturated by water absorption, and carrying out high-temperature and high-pressure heating in a closed container at a heating temperature of 120-190 DEG C and an absolute pressure of 130-320 KPa so as to break the wall of plant cells and vaporize the cell sap; s2) high-temperature negative-pressure heavy metal and drug residue separation; s3) starting a vacuum pump to reduce the pressure of the container, and controlling the temperature in the container to be 110-180 DEG C, so that the cell sap is continuously evaporated and separated in the container, and liquid drops are formed at the top and fall into a containing cup in the container; and S4) reducing the pressure of the container to 100-1000 Pa, and controlling the internal temperature of the container to 105-175 DEG C, so that the cell sap is stably purified and forms the small molecular group cell sap. According to the method disclosed by the invention, the drug residue separation and the small molecular group extraction are completed in the negative pressure environment, and the decomposition of typical substances in the cell sap is reduced under the negative pressure condition, so that the extracting solution keeps the original character characteristics as much as possible.

The invention discloses an adherent cell culture fermentation tank. The fermentation tank comprises a fermentation tank protection shell, a fermentation tank body and a cell counting mechanism, the cell counting mechanism is located at the position, close to the middle, of the front end of the fermentation tank protection shell, the cell counting mechanism penetrates through a cavity to the interior of the fermentation tank body, and the fermentation tank body is located in the fermentation tank protection shell; a fixing ring, a first guide pipe, a conveying pipe, a stirring rod, a stirring shaft, a plurality of groups of stirring blades and a downward extending shaft body are matched for use; the shearing effect of a stirring device on cells in a traditional scheme is reduced; the cell counting mechanism is convenient for people to control different culture stages in the cell culture process; different substances are conveyed through different pipelines connected with a first valve,it is guaranteed that only the required substances are conveyed each time for cell culture, the probability of confusion between the materials is reduced, it is beneficial for changing a single variable to conduct a pre-experiment on cell culture, and the cell culture process is optimized.

The invention discloses a method for preparing feeder layer cells by a suspension-adhesion method. The method comprises the following three steps: obtaining a CF-1 MEFs primary generation; carrying out subculture of the CF-1 MEFs and preparing a CF-1 MEF Feeder. The CF-1 MEF Feeder is prepared by using the suspension-adhesion method which comprises the following steps: digesting the third generation of the CF-1 MEFs cells to form a single-cell suspension; then, bespreading to a culture bottle / vessel at the density of bespreading the culture bottle / vessel after the cells are adhered to the wall; adding MMC for treating for 0.5-3.5 hours after adhesion treatment in a culture box; and quickly absorbing a MMC-containing culture liquid and non-adhered cells in the culture vessel and just recovering adhered cells as the Feeder. The method disclosed by the invention not only can be used for fully inhibiting mitosis, but also can be used for screening cells in good states as the Feeder, so that the quality of the Feeder is improved. Meanwhile, the method can be used for overcoming the defect that the optimum treatment time is hardly mastered as the proliferation speed of MEFs is too fast, so that the preparation time of the CF-1 MEF Feeder is flexible and convenient.

The invention discloses a quality standard detection method for gastrodia elata wall-broken decoction pieces, and the method comprises gastrodia elata wall-broken decoction piece identification, particle size distribution inspection, non-wall-broken cell limit inspection, appearance uniformity inspection, characteristic spectrum determination and content determination. According to the invention,a systematic and scientific quality standard detection method is provided for the gastrodia elata wall-broken decoction pieces, and the method comprises the steps of category identification, inspection of various physicochemical indexes and determination of pharmacological components. According to the detection method, parameters are accurately controlled, the quality of the gastrodia elata wall-broken decoction pieces can be completely reflected, full-component extraction of the gastrodia elata wall-broken decoction pieces can be achieved, the product quality can be more effectively controlled, and the detection method serves as an evaluation basis for the curative effect consistency of the gastrodia elata wall-broken decoction pieces produced on a large scale and has high scientific guidance and industrial regulation and control value.

The invention belonging to the technical field of toothpaste discloses medicinal toothpaste capable of resisting helicobacter pylori. The medicinal toothpaste is prepared from the following functionalbasic raw materials, by weight and an added anti-helicobacter pylori medicine raw material; the functional basic raw materials comprise 60-68 parts of a humectant, 13-17 parts of an abrasive, 0.1-0.4part of an adhesive, 2.6-3.0 parts of a foaming agent, 2.2-2.7 parts of a thickening agent, 1.2-1.8 parts of a sweetening agent and 0.6-1.0 part of a flavoring agent; the anti-helicobacter pylori medicine raw material is prepared from the following raw materials, by weight: 0.2 to 0.6 part of metronidazole, 0.03 to 0.08 part of clarithromycin, 2.1 to 2.7 parts of omeprazole and 0.3 to 0.9 part ofan organic colloidal bismuth agent. A trace amount of clarithromycin is specially used for removing helicobacter pylori and a function of preventing wall cells from transferring hydrogen ions is matched, so that secretion of gastric acid is hindered, mucoprotein in gastric mucosa is prevented from being hydrolyzed, and thus a formed membrane barrier is firm and durable.

The invention provides a human primary cell culture method, and relates to the technical field of cell culture. In the human primary cell culture process, the supernatant of the suspected polluted cell culture solution is subjected to bacterial identification and drug sensitivity detection, and the types and concentrations of antibiotics which have no influence on the cell growth are selected according to the drug sensitivity test result after the cell culture is polluted, so that the bacterial pollution can be reversed in the early stage; the success rate of primary cell culture is greatly improved, and the method plays an important role in timely rescue of precious adherent cell pollution in cell culture.

The invention discloses an efficient multiplication CTL preparation method killing tumors in a targeted mode. The CTL preparation method comprises the following steps: (a) removing CD4+CD25+Treg cells through immunomagnetic bead negative sorting; (b) arranging mixed cells in a serum-free medium for cultivation, and obtaining suspension cells and adherent cells; (c) adding GM-SCF and IL-4 in the adherent cells, culturing the cells for five days; in the sixth day, adding a tumour cell holoantigen, and in the seventh day, adding TNF-alpha and IL-27; (d) transferring the suspension cells to a culture flask wrapped by a CD3 monoclonal antibody and recombinant human fibronectin, adding IFN-gamma, in the second day, adding IL-2, IL-12 and the IL-27, and culturing the mixture till the eighth day to obtain CIK cells; (e) mixing the CIK cells and mature DC cells, and adding the IL-12, IL-7 and an anti-CD 28 monoclonal antibody for cultivation; in the third day, adding an anti-CTLA-4 monoclonal antibody, and then culturing the mixture for four days. According to the efficient multiplication CTL preparation method killing tumors in the targeted mode, efficiency of in-vitro CTL cell proliferation is improved, activity of killing the tumor cells in the targeted mode is improved, transformation of peripheral blood mononuclear cells to the CD4+CD25+Treg cells is inhibited.