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64 results about "Oxyntic cell" patented technology

Alternative Titles: delomorphous cell, oxyntic cell. Parietal cell, also called Oxyntic Cell, or Delomorphous Cell, in biology, one of the cells that are the source of the hydrochloric acid and most of the water in the stomach juices.

Stem cell culture medium and method for culturing endometrium stem cells

The invention provides a stem cell culture medium and a method for culturing endometrium stem cells by using the stem cell culture medium. The method comprises the following steps: separately collecting menstrual blood and endometrium tissues, respectively culturing the menstrual blood and endometrium tissues in the stem cell culture medium provided by the invention to respectively obtain menstrual blood adherent cells and endometrium adherent cells, culturing the menstrual blood adherent cells and endometrium adherent cells in a cell culture bottle, collecting the adherent cells by trypsinization, inoculating the adherent cells in a cell coculture dish, and culturing the adherent cells in the stem cell culture medium provided by the invention. The stem cell culture medium has the advantages of simple components, fewer added components and lower cost. After more than 20 generations of in-vitro culture, the cells can not easily have the phenomenon of aging or degeneration, and can maintain the activity and stem property of the stem cells for a long time. The stem cell culture method is simple and effective, the cell proliferation efficiency is high, and the in-vitro culture doubling time is only 20 hours or so. The cells can be stably amplified by 50 generations.
Owner:HANGZHOU S EVANS BIOSCI LTD

Method of preparing microfibrillated cellulose from bamboo parenchyma cells

InactiveCN104831572ARealize full utilization of multiple componentsAchieve full conversionPulping with organic solventsCelluloseParenchyma
The invention discloses a method of preparing microfibrillated cellulose from bamboo parenchyma cells, which comprises following steps: (1) separation of the bamboo parenchyma cells: performing chemical separation or drying and crushing process to raw materials, sieving the treated raw materials through a 20-mesh sieve screen to obtain the parenchyma cells; (2) chemical pre-treatment: adding the parenchyma cells to a phenethyl alcohol solution for extraction, adding a 1-3% sodium chlorite solution for treating the parenchyma cells for one hour, and adding the parenchyma cells in an alkaline solution for treating the parenchyma cells for two hours, treating the parenchyma cells in a diluted hydrochloric acid solution for two hours, and finally washing the parenchyma cells with deionized water to neutralization; and (3) dispersing the treated parenchyma cells in deionized water to form a 1% suspension liquid, and performing high-frequency ultrasonic treatment or high-pressure homogenizing treatment. According to the method, the bamboo parenchyma cells are separated through the sieving process in early stage to prepare the microfibrillated cellulose. The raw materials are wide in sources and are low in cost. The method is simple and easy to carry out, is high in production efficiency, can reach 90% in yield and can basically achieve all-conversion of the raw materials.
Owner:INT CENT FOR BAMBOO & RATTAN

Functions and application of responsive to centrifugal force and shear stress gene 1 (RECS1) to treatment of restenosis after vascular injury

ActiveCN104198697APromote restenosisPro-restenosisGenetic material ingredientsSurgeryCell phenotypeDisease
The invention discloses functions and application of responsive to centrifugal force and shear stress gene 1 (RECS1) to treatment of restenosis after vascular injury. An RECS1 knockout mouse and a wild type mouse are taken as experimental subjects, and detection on mouse internal membrane regeneration, vessel wall cell proliferation level and smooth muscle cell phenotype transformation is carried out by a vascular injury model. Detection results prove that by virtue of RECS1 knockout, internal membrane regeneration and cell proliferation can be obviously inhibited, and smooth muscle cells are inhibited to be transformed into synthetic phenotype from contractile phenotype. Therefore, the functions of the RECS1 to the restenosis after vascular injury are mainly embodied in that internal membrane regeneration, cell proliferation and smooth muscle cell phenotype transformation are promoted by the RECS1. Aiming at the functions of the RECS1, the RECS1 can be used as a drug target for screening medicines for preventing, relieving and/or treating the disease of restenosis after vascular injury; an inhibitor of the RECS1 can be used for preparing medicines and arterial stents for preventing, relieving and/or treating the restenosis after vascular injury.
Owner:武汉惠康基因科技有限公司

Immune cell and preparation method thereof

ActiveCN105039254AStrong tumor antigen sensitizationStrong antigen sensitizationBlood/immune system cellsAntigenRisk factor
The invention relates to the technical field of biology, in particular to an immune cell and a preparation method thereof. The preparation method comprises the steps that tumor cell nucleotide is mixed with supernate, and a first preparation is obtained; PBMC is taken for culture, and a suspension cell and an adherent cell are separated; the adherent cell is cultured through a culture medium containing the first preparation, and a DC cell is obtained; induced culture is conducted on the suspension cell, and an immune cell with killing efficiency is obtained; the DC cell and the immune cell with the killing efficiency are cultured together, and the immune cell is prepared. According to the the immune cell and the preparation method thereof, the tumor cell nucleotide and protein allergic sources are utilized simultaneously, strengthening stimulation is conducted on the DC cell, stronger antigen sensitization is exerted, higher affinity with a tumor cell is achieved, and the risk factor of the tumor cell does not exist.
Owner:GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD

Suspension culture method of adherent cells and application thereof

The invention discloses a suspension culture method of adherent cells and application thereof. The suspension culture method and the application provide modified adherent cells by high expression of transformation regulation medium matters related to cell suspension culture in the adherent cells; and then the modified adherent cells are cultured by the suspension culture method. The transformationregulation medium matters related to the cell suspension culture are subjected to higher level expression in the adherent cells, and the modified adherent cells are not prone to clustering in suspension culture, the improvement of cell survival rate is facilitated, the expression of foreign proteins or the preparation of virus vaccines is facilitated, and the production efficiency is improved. Therefore, the suspension culture method is particularly suitable for the production of recombinant proteins or the virus vaccines.
Owner:广州创瑞健康科技有限公司

Plant cell sap extraction device and method

The invention discloses a plant cell sap extraction method and device, and the extraction method comprises the following steps: S1) taking fresh plant fragments or a to-be-extracted product saturated by water absorption, and carrying out high-temperature and high-pressure heating in a closed container at a heating temperature of 120-190 DEG C and an absolute pressure of 130-320 KPa so as to break the wall of plant cells and vaporize the cell sap; s2) high-temperature negative-pressure heavy metal and drug residue separation; s3) starting a vacuum pump to reduce the pressure of the container, and controlling the temperature in the container to be 110-180 DEG C, so that the cell sap is continuously evaporated and separated in the container, and liquid drops are formed at the top and fall into a containing cup in the container; and S4) reducing the pressure of the container to 100-1000 Pa, and controlling the internal temperature of the container to 105-175 DEG C, so that the cell sap is stably purified and forms the small molecular group cell sap. According to the method disclosed by the invention, the drug residue separation and the small molecular group extraction are completed in the negative pressure environment, and the decomposition of typical substances in the cell sap is reduced under the negative pressure condition, so that the extracting solution keeps the original character characteristics as much as possible.
Owner:梁小毛

Adherent cell culture fermentation tank

The invention discloses an adherent cell culture fermentation tank. The fermentation tank comprises a fermentation tank protection shell, a fermentation tank body and a cell counting mechanism, the cell counting mechanism is located at the position, close to the middle, of the front end of the fermentation tank protection shell, the cell counting mechanism penetrates through a cavity to the interior of the fermentation tank body, and the fermentation tank body is located in the fermentation tank protection shell; a fixing ring, a first guide pipe, a conveying pipe, a stirring rod, a stirring shaft, a plurality of groups of stirring blades and a downward extending shaft body are matched for use; the shearing effect of a stirring device on cells in a traditional scheme is reduced; the cell counting mechanism is convenient for people to control different culture stages in the cell culture process; different substances are conveyed through different pipelines connected with a first valve,it is guaranteed that only the required substances are conveyed each time for cell culture, the probability of confusion between the materials is reduced, it is beneficial for changing a single variable to conduct a pre-experiment on cell culture, and the cell culture process is optimized.
Owner:陈红

Quality standard detection method for gastrodia elata wall-broken decoction pieces

The invention discloses a quality standard detection method for gastrodia elata wall-broken decoction pieces, and the method comprises gastrodia elata wall-broken decoction piece identification, particle size distribution inspection, non-wall-broken cell limit inspection, appearance uniformity inspection, characteristic spectrum determination and content determination. According to the invention,a systematic and scientific quality standard detection method is provided for the gastrodia elata wall-broken decoction pieces, and the method comprises the steps of category identification, inspection of various physicochemical indexes and determination of pharmacological components. According to the detection method, parameters are accurately controlled, the quality of the gastrodia elata wall-broken decoction pieces can be completely reflected, full-component extraction of the gastrodia elata wall-broken decoction pieces can be achieved, the product quality can be more effectively controlled, and the detection method serves as an evaluation basis for the curative effect consistency of the gastrodia elata wall-broken decoction pieces produced on a large scale and has high scientific guidance and industrial regulation and control value.
Owner:ANHUI GUANGYINTANG CHINESE MEDICINE

Medicinal toothpaste capable of resisting helicobacter pylori

InactiveCN111494232APrevent transport functionPrevent secretionAntibacterial agentsOrganic active ingredientsBiotechnologyToothpaste
The invention belonging to the technical field of toothpaste discloses medicinal toothpaste capable of resisting helicobacter pylori. The medicinal toothpaste is prepared from the following functionalbasic raw materials, by weight and an added anti-helicobacter pylori medicine raw material; the functional basic raw materials comprise 60-68 parts of a humectant, 13-17 parts of an abrasive, 0.1-0.4part of an adhesive, 2.6-3.0 parts of a foaming agent, 2.2-2.7 parts of a thickening agent, 1.2-1.8 parts of a sweetening agent and 0.6-1.0 part of a flavoring agent; the anti-helicobacter pylori medicine raw material is prepared from the following raw materials, by weight: 0.2 to 0.6 part of metronidazole, 0.03 to 0.08 part of clarithromycin, 2.1 to 2.7 parts of omeprazole and 0.3 to 0.9 part ofan organic colloidal bismuth agent. A trace amount of clarithromycin is specially used for removing helicobacter pylori and a function of preventing wall cells from transferring hydrogen ions is matched, so that secretion of gastric acid is hindered, mucoprotein in gastric mucosa is prevented from being hydrolyzed, and thus a formed membrane barrier is firm and durable.
Owner:苏州聚分享电子商贸有限公司

A CTL preparation method for efficient proliferation and targeted killing of tumors

The invention discloses an efficient multiplication CTL preparation method killing tumors in a targeted mode. The CTL preparation method comprises the following steps: (a) removing CD4+CD25+Treg cells through immunomagnetic bead negative sorting; (b) arranging mixed cells in a serum-free medium for cultivation, and obtaining suspension cells and adherent cells; (c) adding GM-SCF and IL-4 in the adherent cells, culturing the cells for five days; in the sixth day, adding a tumour cell holoantigen, and in the seventh day, adding TNF-alpha and IL-27; (d) transferring the suspension cells to a culture flask wrapped by a CD3 monoclonal antibody and recombinant human fibronectin, adding IFN-gamma, in the second day, adding IL-2, IL-12 and the IL-27, and culturing the mixture till the eighth day to obtain CIK cells; (e) mixing the CIK cells and mature DC cells, and adding the IL-12, IL-7 and an anti-CD 28 monoclonal antibody for cultivation; in the third day, adding an anti-CTLA-4 monoclonal antibody, and then culturing the mixture for four days. According to the efficient multiplication CTL preparation method killing tumors in the targeted mode, efficiency of in-vitro CTL cell proliferation is improved, activity of killing the tumor cells in the targeted mode is improved, transformation of peripheral blood mononuclear cells to the CD4+CD25+Treg cells is inhibited.
Owner:四川全组生命科技有限公司

A Chinese medicinal composition with qi promote and stomach promoting effect, and its preparation method and application

ActiveCN109224025ASignificantly removes dampness and invigorates the spleenReduce moisture contentDigestive systemFungi medical ingredientsTotal proteinOfficinalis
The invention relates to the technical field of traditional Chinese medicine, in particular to a Chinese medicinal composition with dampness removing, spleen transporting, qi promoting and stomach promoting effects, as well as a preparation method and application thereof. The Chinese medicinal composition is prepared by 2-7 part of rhizoma Atractylodis, 2-7 parts of rhizoma Atractylodis Macrocephalae, 1-5 part of cortex Magnoliae officinalis, 1-5 parts of pericarpium Citri Tangerinae, 1-5 parts of Poria, 1-5 parts of rhizoma Alismatis, 1-5 part of Polyporus, 1-2 parts of radix aucklandiae, 1-2parts of fructus Amomi, 1-2 parts of cortex Cinnamomi and 1-2 parts of radix Glycyrrhizae. As that Chinese medicinal material are combined accord to a specific proportion, the obtained composition can obviously reduce fecal water content, regulate the symptom of spleen deficiency and dampness retention, and simultaneously promote urination of rats with spleen deficiency and dampness retention andreduce the occurrence of edema. It can also promote the absorption of serum total protein and albumin, regulate the secretion of gastric acid, promote the growth of gastric mucosa and parietal cells,remove dampness and transport spleen, promote qi and stomach to regulate the function of gastrointestinal tract.
Owner:吉林修正药业新药开发有限公司 +2
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