19 results about "Oxyntic cell" patented technology

The invention belongs to the technical field of medicines, and particularly provides a solid dispersion as well as a preparation method and application thereof. The solid dispersion comprises a carrier material, and further comprises quercetagetin and phospholipid, the mass ratio of the quercetagetin to the phospholipid is 1: (0.3-1.5), and the carrier material comprises one or more of a hydrophilic polymer and a pH-dependent polymer. Wherein the quercetagetin and the phospholipid are combined through an intermolecular force, so that the quercetagetin obtains a lipid material similar to a cell membrane structure, a cell membrane bionic phospholipid compound is formed, the cell membrane bionic phospholipid compound can be naturally compatible with an upper wall cell membrane of a small intestine, and the permeation transmembrane capability of the quercetagetin is better promoted; the quercetagetin is added into the solid dispersion, so that more quercetagetin can enter systemic circulation and lymphatic circulation, and therefore, the solid dispersion can strengthen transmembrane absorption while strengthening dissolution, so that the solid dispersion has high bioavailability.

The mechanisms driving the development of extracapillary lesions in focal segmental glomerulosclerosis (FSGS) and crescentic glomerulonephritis (CGN) remain poorly understood. A key question is how parietal epithelial cells (PECs) invade glomerular capillaries, thereby promoting injury and kidney failure. Here the inventors show that expression of the tetraspanin CD9 increases markedly in PECs in mouse models of CGN and FSGS, and in kidneys from individuals diagnosed with these diseases. Cd9 gene targeting in PECs prevents glomerular damage in CGN and FSGS mouse models. Mechanistically, CD9 deficiency prevents the oriented migration of PECs into the glomerular tuft and their acquisition of CD44 and β1 integrin expression. These findings highlight a critical role for de novo expression of CD9 as a common pathogenic switch driving the PEC phenotype in CGN and FSGS, while offering a potential therapeutic avenue to treat these conditions. Accordingly, CD9 represents a reliable biomarker and as well as a biotargets in glomerulonephritides.

The invention discloses a multilayer adherent cell culture reactor and a use method. The culture reactor comprises a shell, a plurality of layers of cell culture plates are arranged in the shell in an array manner; and the liquid inlet is used as an inlet of a cell suspension. The liquid outlet is an outlet of the culture medium; the air holes are formed in the upper surface and the lower surface of the shell. And the quality control chamber is communicated with the inner cavity of the shell. The use method comprises the following steps: uniformly mixed cells and a culture medium enter the inner cavity of the shell through the liquid inlet for positive culture; after front culture is completed, the multi-layer adherent cell culture device is inverted; uniformly mixed cells and a culture medium enter the inner cavity of the shell through the liquid inlet for reverse culture. By arranging the multiple layers of cell culture plates with double surfaces capable of culturing, the cell culture area is greatly increased, the space utilization rate is improved, the use amount of a culture medium is reduced, the prepared quality control chamber is communicated with the inner cavity of the shell, and the cell growth state is conveniently, rapidly and accurately observed on the premise of not polluting the internal environment and not damaging the culture stability through isolation of the partition opening.

The disclosure is directed to a method for seed train expansion of adherent cells comprising culturing cells with a serum-supplemented growth medium in a N-2 vessel; removing the cells from the serum-supplemented medium; inoculating the cells from step into a serum-free growth medium in a N-1 vessel; culturing the cells in the N-1 vessel under suspension conditions; and inoculating a growth medium in a bioreactor with the suspension-cultured cells. In some aspects, the adherent cells are not suspension-adapted. In some aspects, the adherent cells are suspension-adapted. In some aspects, the adherent cells produced by the seed train expansion method are used to produce viral vectors. In some aspects, the viral vectors are AAV vectors.

The invention relates to the technical field of biological medicine, and discloses a natural product for targeted inhibition of a KCNQ1 / KCNE2 complex channel and application of the natural product for targeted inhibition of the KCNQ1 / KCNE2 complex channel in preparation of a medicine for treating or preventing gastric ulcer, and an application method of a pharmaceutical composition comprises the following steps: S1, applying the pharmaceutical composition to a medication object; s2, the natural product interacts with a KCNQ1 / KCNE2 complex channel on a gastric wall cell membrane, and potassium ion current of the complex channel is inhibited; s3, weakening functions of H < + > / K < + >-ATPase in gastric wall cells by inhibiting potassium ion current, so that gastric acid secretion is reduced, and gastric ulcer is treated or prevented. According to the invention, gastric acid is indirectly regulated and controlled through targeted inhibition of gastric wall cell KCNQ1 / KCNE2 complex channels, and a new strategy for treating gastric ulcer, which is milder and has safety potential, is provided.

The present application relates to the technical field of biology, and particularly discloses a method for culturing and obtaining foregut endoderm cell spheroids and application thereof. The method for culturing and obtaining foregut endoderm cell spheroids of the present application obtains foregut endoderm cell spheroids by inducing differentiation of stem cells on a culture surface with a PDA-matrigel coating. The culture method of the present application can ensure the differentiation efficiency of DE and AFE adherent cells and spheroids, promote the generation of AFE cell spheroids and the separation from the adherent cell layer, reduce the edge rolling of AFE adherent cells, prolong the production time of AFE cell spheroids, significantly improve the yield of AFE cell spheroids, and provide a basis for subsequent differentiation and culture of lung organoids.

An adherent cell culture substrate, which is a sheet-shaped substrate used for producing a culture vessel for adherent cells, includes a groove including a crest-shaped portion and a valley-shaped portion on one surface side of the substrate, in which a distance from a top end portion of the crest-shaped portion to the other surface of the substrate is 1 mm or less. Further, it is preferable that a cross section of the groove perpendicular to a direction in which the groove extends is formed in a substantially V-shape and an inclination angle of a side surface of the substantially V-shape of the groove is 80 degrees or less. Further, it is preferable that a plurality of the grooves are linearly arranged in parallel and the top end portions of the crest-shaped portions are formed in a linear shape.

The invention relates to an adherent cell microcarrier suspension culture method, which can reduce the usage amount of serum, reduce the cost of a culture medium and the risk of biosafety, can flexibly enlarge the culture volume according to the specification of a shake flask, and is convenient to operate.

The invention relates to the technical field of surface plasma resonance, and discloses an establishment method and application of an intestinal wall cell mucous layer model. The method comprises the following steps: carrying out bionic treatment on the surface of a metasurface plasmon resonance chip, inputting a culture solution containing intestinal wall cells into the surface of the metasurface plasmon resonance chip through a micro-fluidic chip to carry out intestinal wall cell culture, inputting an intestinal tract simulation solution into the surface of the metasurface plasmon resonance chip, and carrying out intestinal tract simulation on the surface of the metasurface plasmon resonance chip. And detecting the refractive index change of the surface of the metasurface plasmon resonance chip, and monitoring the forming process of the mucus layer of the intestinal wall cell in real time according to the refractive index change. According to the intestinal wall cell mucous layer model obtained through the construction method, unmarked real-time continuous monitoring of the intestinal wall cell mucous layer forming process, dynamic observation of physiological changes of cells and simulation of the real intestinal environment can be achieved, and the method has high sensitivity and accuracy, is low in detection cost and has remarkable market prospects.

The invention belongs to the technical field of cell clone acquisition, and discloses a needle head, a teasing needle and an adherent cell clone acquisition method.The needle head comprises a needle head body and a supporting column, the needle head body is provided with a suction channel, and the suction channel penetrates through the first end face, in the height direction, of the needle head body; the multiple supporting columns are arranged on the outer surface of the needle head body at intervals in the circumferential direction of the needle head body, and the supporting columns protrude out of the first end face; the adherent cell clone obtaining method comprises the steps that when cell clone obtaining is conducted, the needle body is placed in a culture solution of a culture container, all the supporting columns abut against the inner wall of the culture container, a flow field is more stable, then cell clone is sucked through the suction channel, and therefore when the cell clone is sucked, the cell clone is separated from the culture solution, and the adherent cell clone is obtained. The distance between the first end face and cell clone does not need to be noticed; meanwhile, the needle head can also form a relatively stable flow field on the surface, so that the acquisition efficiency of adherent cell clone is improved.

The invention relates to the field of biomedical engineering, and discloses a construction method and application of an intestinal wall cell barrier model. The method comprises the following steps: performing intestinal wall cell culture on a metasurface plasmon resonance chip of which the surface is subjected to bionic treatment in a culture medium to obtain a chip plate attached to the wall of intestinal wall cells, and monitoring the forming process of a barrier layer of the intestinal wall cells in real time by detecting the refractive index change of the surface of the metasurface plasmon resonance chip. The intestinal wall cell barrier model obtained by the construction method can realize unmarked real-time continuous monitoring of the formation process of the intestinal wall cell barrier layer and dynamic observation of physiological changes of cells, and the method has high sensitivity and stability.

The utility model discloses a bioreactor microcarrier adherent cell pancreatin digestion device, which belongs to the technical field of cell pancreatin digestion, and comprises a pancreatin digestion unit connected with a bioreactor, and the pancreatin digestion unit is connected with a bottom valve of the bioreactor through a second connecting pipe; the micro-carrier adherent cells are digested by pancreatin in the bioreactor. The bioreactor microcarrier adherent cell pancreatin digestion device provided by the utility model can be used for observing the cell digestion state in real time and terminating digestion in time, carrying out data statistics and analysis on cell counting, calculating the recovery rate of digested cells, and observing the cell attachment rate and the cell growth condition of the cells in a next-stage bioreactor, so that the digestion efficiency of the cells in the next-stage bioreactor is improved. Compared with culture parameter data of a production batch for cell digestion by using a butterfly-shaped pancreatin digester before improvement, the recovery rate and the adherence rate are stably increased, and the working efficiency and the product yield are better improved.

One embodiment of this invention relates to a method for manufacturing a cell suspension including (A), (B) and (C).　(A) culturing adherent cells in a cell suspension containing the adherent cells, a microcarrier, and a medium and having a volume of 0.3 L or more; (B) culturing the adherent cells in a cell suspension containing the adherent cells, the microcarrier, and the medium obtained by (A) above and having a volume of 5 L or more; and (C) culturing the adherent cells in a cell suspension containing the adherent cells, the microcarrier, and the medium obtained by (B) above and having a volume of 10 L or more.

The utility model relates to the technical field of adherent cell culture bottle, concretely is a kind of adherent cell culture bottle convenient to change liquid, including box body subassembly, closed mechanism is installed on the box body subassembly;The lower end of the box body subassembly is equipped with connecting mechanism;The lower end middle part of the box body subassembly is equipped with first installation slot, through pipe is equipped in the inner bottom of the box body subassembly, the lower end of the through pipe extends into first installation slot, and one end of the through pipe in first installation slot is connected with discharge pipe.The application can be convenient to change liquid operation by closed mechanism, and it is also convenient to connect between bottle body, to facilitate storage and storage, and the stored liquid is discharged by discharge pipe, and liquid can be effectively discharged.

The invention discloses an extraction and culture method of central nervous system vascular wall cells, and relates to the technical field of cell culture. Microvascular clumps are separated through one-step enzymolysis in combination with two-time centrifugation and four-time natural settling, a large number of high-purity and high-activity vascular wall cells are obtained through in-vitro culture and five-time passage, and the method has the advantages of being easy and convenient to operate, low in cost, high in efficiency, good in cell activity and high in purity. The method comprises the following steps: S1, carrying out enzymatic dissociation on obtained brain and spinal cord tissues by using papain; s2, blowing and beating brain and spinal cord tissues to obtain brain and spinal cord tissue homogenate; s3, adding bovine serum albumin into the brain and spinal cord tissue homogenate, and centrifuging twice to obtain a low-purity microvascular plexus; s4, naturally settling the low-purity capillary plexus for four times to obtain a high-purity capillary plexus; s5, inoculating the high-purity capillary plexus into a culture plate; s6, performing in-vitro culture to enable the cells to climb out of the capillary plexus to obtain vascular wall cells; and S7, carrying out passage for five times to obtain the high-purity vascular wall cells.

The utility model relates to the technical field of cell culture bottles, in particular to an adherent cell culture bottle which comprises a bottle body for containing culture solution, a dynamic supporting piece and a stabilizing piece, the dynamic supporting piece is arranged at the bottom of the bottle body and used for being in contact with a table top, and when the bottle body is subjected to external driving force, the dynamic supporting piece is fixed on the bottle body. The dynamic supporting piece is arranged on the bottle body, the dynamic supporting piece drives the bottle body to shake so that a culture solution in the bottle body can be in a uniform and efficient mixed state, and the stabilizing piece is arranged on the bottle body and used for erecting the dynamic supporting piece in a non-operation state so that the bottle body can be stably placed. The bottle body can more smoothly roll on the table top, an operator only needs to slightly apply force to push the bottle body to shake with the dynamic supporting piece as a fulcrum, and compared with a traditional plane bottom culture bottle which needs to be suspended and shaken vigorously in the whole process, the operation difficulty is reduced.

The application discloses an in-situ freezing method for adherent cells, and the method obtains a "sandwich layer" structure by using Matrigel and gelatin before freezing; the bottom layer can prevent the middle layer of cells from falling off due to temperature change and prevent the cells from being frozen; the uppermost layer is used to protect the middle layer of cells from being frozen, and can be removed well during rewarming, thereby facilitating use and subculture operation. The method does not need to digest cells and collect cells, and avoids damage to cells caused by operations such as digestion and blowing before freezing; the method solves the problems of limitation of adherent materials and falling off of adherent cells caused by non-affinity materials in the in-situ freezing of adherent cells; after thawing and recovery, the cells do not need to be re-inoculated and subcultured for a long time to recover the cell state, and the cells can be used after thawing, thereby shortening the culture time of the cells after recovery; the survival rate of the adherent cells after recovery can reach 73.32%, the survival rate is good, the adherent state after recovery is good, there is no obvious falling off, and the cells have normal morphology.

The utility model relates to the technical field of cell culture, in particular to a culture unit for high-density adherent cells, which comprises a culture bag, a carrier unit mounted in the middle of the inside of the culture bag, a perfusion inlet pipe connected to one side of the bottom of the culture bag, a perfusion outlet pipe connected to one side of the top of the culture bag, and a breather pipe connected to the bottom of the culture bag. The top of the culture bag is connected with an air outlet pipe. In the culture unit of the high-density adherent cells, the carrier unit is arranged in the culture bag, and the unique lamination groove structure and the side wall opening design of the carrier unit remarkably improve the adherent efficiency and growth density of the cells, so that the cells can grow in a more uniform environment. Due to the design of the top cover at the top of the culture bag and the connection of the locking plate and the locking bolt between the top cover and the culture bag, the sealing performance and the stability in the culture process are ensured. And the sealing groove and the sealing ring are arranged at the bottom of the top cover, so that the sealing effect is further improved, and external pollutants are prevented from entering.

The application discloses a human allogeneic acellular matrix tissue-engineered heart valve, a preparation method and application. A composite tubular support and / or a composite film of polyglycolic acid and polyethylene glycol polymers is prepared by electrostatic spinning, seed cells are planted in the composite tubular support and / or the composite film, perfusion culture is carried out under first preset culture conditions to simulate arterial pulsation flow pressure and axial cyclic tensile stress conditions of a human body, a tubular and / or sheet-shaped engineered tissue composed of the seed cells and extracellular matrix is obtained, the tubular and / or sheet-shaped engineered tissue is decellularized to remove cells in the tubular wall, a tubular engineered tissue and / or a sheet-shaped engineered film composed of extracellular matrix is obtained, and the tubular engineered tissue and / or the sheet-shaped engineered film is cut to obtain acellular matrix tissue-engineered heart valve leaflet material. The acellular matrix tissue-engineered heart valve leaflet material is used to prepare an acellular matrix tissue-engineered heart valve including a conventional surgical heart valve and a minimally invasive interventional heart valve.