65 results about "Oxyntic cell" patented technology

The invention provides a stem cell culture medium and a method for culturing endometrium stem cells by using the stem cell culture medium. The method comprises the following steps: separately collecting menstrual blood and endometrium tissues, respectively culturing the menstrual blood and endometrium tissues in the stem cell culture medium provided by the invention to respectively obtain menstrual blood adherent cells and endometrium adherent cells, culturing the menstrual blood adherent cells and endometrium adherent cells in a cell culture bottle, collecting the adherent cells by trypsinization, inoculating the adherent cells in a cell coculture dish, and culturing the adherent cells in the stem cell culture medium provided by the invention. The stem cell culture medium has the advantages of simple components, fewer added components and lower cost. After more than 20 generations of in-vitro culture, the cells can not easily have the phenomenon of aging or degeneration, and can maintain the activity and stem property of the stem cells for a long time. The stem cell culture method is simple and effective, the cell proliferation efficiency is high, and the in-vitro culture doubling time is only 20 hours or so. The cells can be stably amplified by 50 generations.

The invention discloses a method of preparing microfibrillated cellulose from bamboo parenchyma cells, which comprises following steps: (1) separation of the bamboo parenchyma cells: performing chemical separation or drying and crushing process to raw materials, sieving the treated raw materials through a 20-mesh sieve screen to obtain the parenchyma cells; (2) chemical pre-treatment: adding the parenchyma cells to a phenethyl alcohol solution for extraction, adding a 1-3% sodium chlorite solution for treating the parenchyma cells for one hour, and adding the parenchyma cells in an alkaline solution for treating the parenchyma cells for two hours, treating the parenchyma cells in a diluted hydrochloric acid solution for two hours, and finally washing the parenchyma cells with deionized water to neutralization; and (3) dispersing the treated parenchyma cells in deionized water to form a 1% suspension liquid, and performing high-frequency ultrasonic treatment or high-pressure homogenizing treatment. According to the method, the bamboo parenchyma cells are separated through the sieving process in early stage to prepare the microfibrillated cellulose. The raw materials are wide in sources and are low in cost. The method is simple and easy to carry out, is high in production efficiency, can reach 90% in yield and can basically achieve all-conversion of the raw materials.

The invention relates to a laboratory adherent cell thin film culture device and the method of the field of biological engineering technology, and the device includes a flat dish, an organic polymer thin film layer and an adhesive liquid layer. The organic polymer thin film layer is adhered at the inner bottom surface of the flat dish. The polymer material thin film is wetted with the adhesive liquid to be adhered at the bottom surface of a culture dish as an adherent cell attachment surface, so as to facilitate the rapid separation of the cells during the passage, the cells are rapidly put into a volumetric culture liquid and then are exfoliated by swinging method, further to carry out counting and passage based on this, the accurate component ensures the parallel initial inoculation conditions of the next generation; compared with the original passage method, the operation is more simple, and the bacteria contamination opportunities are fewer. The different polymer thin films can be attached for matching different needs, so as to improve digestive efficiency or survival rate of the adherent culture.

The invention discloses a three-dimensional dyeing method for amygdalus communis l anther cells. According to the three-dimensional dyeing method, an amygdalus communis l anther slice treated with a conventional paraffin slicing method is dyed with an iron haematoxylin solution prepared 10 min in advance, such that the pollen granulocytes, the tapetal cells, the intermediate cells, the connective cells, the endothecium cells, the epidermic cells, the cells at the connection tissue position and the filaments of the amygdalus communis l anther can be clearly colored, and the coloring degrees among the cells at different parts are different so as to provide the three-dimensional dyeing effect. The three-dimensional dyeing method can be used for the study on the three-dimensional dyeing method internal structure change and the anther development process.

The invention discloses functions and application of responsive to centrifugal force and shear stress gene 1 (RECS1) to treatment of restenosis after vascular injury. An RECS1 knockout mouse and a wild type mouse are taken as experimental subjects, and detection on mouse internal membrane regeneration, vessel wall cell proliferation level and smooth muscle cell phenotype transformation is carried out by a vascular injury model. Detection results prove that by virtue of RECS1 knockout, internal membrane regeneration and cell proliferation can be obviously inhibited, and smooth muscle cells are inhibited to be transformed into synthetic phenotype from contractile phenotype. Therefore, the functions of the RECS1 to the restenosis after vascular injury are mainly embodied in that internal membrane regeneration, cell proliferation and smooth muscle cell phenotype transformation are promoted by the RECS1. Aiming at the functions of the RECS1, the RECS1 can be used as a drug target for screening medicines for preventing, relieving and/or treating the disease of restenosis after vascular injury; an inhibitor of the RECS1 can be used for preparing medicines and arterial stents for preventing, relieving and/or treating the restenosis after vascular injury.

The present invention relates to a culture method of hair follicle mesenchymal stem cells and belongs to the field of biology. The culture method of the hair follicle mesenchymal stem cells comprisesthe following steps: step 1, acquiring primary hair follicle tissue blocks; step 2, inoculating the obtained primary hair follicle tissue blocks into a cell culture medium for a culture treatment; andstep 3, carrying out biological identification on amplified cells, wherein results show that (1) the cells are adhered to walls; (2) cell phenotypes are as follows: CD44+ is greater than or equal to95%, CD105+ is greater than or equal to 95%, CD45+ is less than or equal to 2% and CD34+ is less than or equal to 2%; and (3) the cells can be differentiated into adipocytes, osteocytes and chondrocytes. The culture method can effectively improve proliferation of the hair follicle mesenchymal stem cells, improves an amplification capacity of the hair follicle mesenchymal stem cells in a culture process, is convenient to obtain materials, has small pain to obtain the materials, and is rich in sources and simple in separation.

The invention discloses functions and application of SHPS1 in treatment of post-vascular injury restenosis, belonging to the field of functions and applications of genes. SHPS1 gene knockout mice and wild type mice are taken as experimental subjects, and due to a vascular injury model, the mouse neointima formation, vascular wall cell proliferation level and smooth muscle cell phenotype transform are detected. The result proves that SHPS1 gene knockout can obviously promote neointima neogenesis and cell proliferation and promote transformation of smooth muscle cells from a shrinkage manner to a synthetic manner. Thus the functions of SHPS1 in post-vascular injury restenosis are realized, which mainly shows that SHPS1 has the functions of inhibiting neointima formation and cell proliferation and inhibiting smooth muscle cell phenotype transformation. Aiming at the functions of the SHPS1, the SHPS1 can be used for preparing medicines and arterial stents for preventing, relieving and/or treating the post-vascular injury restenosis.

The invention relates to a method for extracting anchorage-dependent cells. The method comprises the following steps of preprocessing polyacrylamide hydrogel, culturing anchorage-dependent cells, integrally excavating the cells and the adhered gel, removing surface gel and the like. According to the method, an anchorage-dependent cell extracting device suitable for the extracting method is also arranged. The extracting method disclosed by the invention is convenient and easy to operate, and the extracting of the anchorage-dependent cells can be completed without tedious steps, so that the possibility of mistaking is reduced; besides, the adopted extracting device is simple in structure and low in cost; and in addition, the extracting method is small in damage to the anchorage-dependent cells, and has important significance to cell research.

The present invention provides a cell carrier containing a nanofiber composed of water-insoluble polysaccharides, preferably chitin or chitosan nanofiber; a carrier capable of being a common carrier in various operations such as i) suspension culture, ii) differentiation induction, iii) transportation and preservation under non-freezing conditions, iv) transplantation, v) recovery of bioactive substance from culture supernatant and the like of adherent cells; and continuous performance of plural operations selected from i) suspension culture, ii) differentiation induction, iii) transportation and preservation under non-freezing conditions, iv) transplantation, and v) recovery of bioactive substance from culture supernatant of adherent cells by using the carrier.

The invention discloses a preparation method of a parenchymal cell cellulose and liquid metal nano-droplet composite membrane, which comprises the following steps: by taking agricultural waste bagasse pith as a source of parenchymal cell cellulose, removing lignin by using a chlorine dioxide method, and then removing hemicellulose by using an organic guanidine aqueous solution method to obtain parenchymal cell cellulose; the method comprises the following steps: dissolving parenchyma cell cellulose through DMAC and LiCl to obtain a parenchyma cell cellulose dissolving solution; the preparation method comprises the following steps: ultrasonically dispersing liquid metal into nano liquid drops by using isopropanol, modifying by using APTES to obtain SML NDs, and finally preparing the composite membrane by using a grafting reaction caused by N, N-methylene bisacrylamide and ammonium persulfate among cellulose macromolecules. The composite film has antibacterial ability and stretchability, also has good physical strength, can be completely degraded, has low toxicity and good skin fitness, and provides a feasible scheme for expanding the application field of bagasse pith and improving the additional value of bagasse pith.

The invention belongs to the technical field of biochemistry, and particularly relates to a digestion staining solution and a crystal violet staining cell nucleus counting method. In order to solve the problems that pancreatin digestive juice digestion time is not easy to control in a sheet-shaped carrier adherent culture mode, and digested cells cannot be completely separated from a net-shaped space in the prior art, the invention provides the digestive staining solution which is a mixed solution of crystal violet, citric acid and triton X100. The crystal violet staining cell nucleus countingmethod comprises the following steps of: (1) mixing a carrier carrying cells with the digestion staining solution, and staining and digesting the mixture to obtain a staining solution; and (2) carrying out cell nucleus counting on the staining solution. The invention further provides application of triton X100 in digesting adherent cells in a cell nucleus counting process. According to the crystal violet staining cell nucleus counting method, the phenomena of cell damage and even death caused by overlong time in the digestion process can be reduced, so that the accuracy of cell counting is improved.

The invention discloses a plant cell sap extraction method and device, and the extraction method comprises the following steps: S1) taking fresh plant fragments or a to-be-extracted product saturated by water absorption, and carrying out high-temperature and high-pressure heating in a closed container at a heating temperature of 120-190 DEG C and an absolute pressure of 130-320 KPa so as to break the wall of plant cells and vaporize the cell sap; s2) high-temperature negative-pressure heavy metal and drug residue separation; s3) starting a vacuum pump to reduce the pressure of the container, and controlling the temperature in the container to be 110-180 DEG C, so that the cell sap is continuously evaporated and separated in the container, and liquid drops are formed at the top and fall into a containing cup in the container; and S4) reducing the pressure of the container to 100-1000 Pa, and controlling the internal temperature of the container to 105-175 DEG C, so that the cell sap is stably purified and forms the small molecular group cell sap. According to the method disclosed by the invention, the drug residue separation and the small molecular group extraction are completed in the negative pressure environment, and the decomposition of typical substances in the cell sap is reduced under the negative pressure condition, so that the extracting solution keeps the original character characteristics as much as possible.

The invention discloses an adherent cell culture fermentation tank. The fermentation tank comprises a fermentation tank protection shell, a fermentation tank body and a cell counting mechanism, the cell counting mechanism is located at the position, close to the middle, of the front end of the fermentation tank protection shell, the cell counting mechanism penetrates through a cavity to the interior of the fermentation tank body, and the fermentation tank body is located in the fermentation tank protection shell; a fixing ring, a first guide pipe, a conveying pipe, a stirring rod, a stirring shaft, a plurality of groups of stirring blades and a downward extending shaft body are matched for use; the shearing effect of a stirring device on cells in a traditional scheme is reduced; the cell counting mechanism is convenient for people to control different culture stages in the cell culture process; different substances are conveyed through different pipelines connected with a first valve,it is guaranteed that only the required substances are conveyed each time for cell culture, the probability of confusion between the materials is reduced, it is beneficial for changing a single variable to conduct a pre-experiment on cell culture, and the cell culture process is optimized.

The invention provides a human primary cell culture method, and relates to the technical field of cell culture. In the human primary cell culture process, the supernatant of the suspected polluted cell culture solution is subjected to bacterial identification and drug sensitivity detection, and the types and concentrations of antibiotics which have no influence on the cell growth are selected according to the drug sensitivity test result after the cell culture is polluted, so that the bacterial pollution can be reversed in the early stage; the success rate of primary cell culture is greatly improved, and the method plays an important role in timely rescue of precious adherent cell pollution in cell culture.

The invention relates to the technical field of traditional Chinese medicine, in particular to a Chinese medicinal composition with dampness removing, spleen transporting, qi promoting and stomach promoting effects, as well as a preparation method and application thereof. The Chinese medicinal composition is prepared by 2-7 part of rhizoma Atractylodis, 2-7 parts of rhizoma Atractylodis Macrocephalae, 1-5 part of cortex Magnoliae officinalis, 1-5 parts of pericarpium Citri Tangerinae, 1-5 parts of Poria, 1-5 parts of rhizoma Alismatis, 1-5 part of Polyporus, 1-2 parts of radix aucklandiae, 1-2parts of fructus Amomi, 1-2 parts of cortex Cinnamomi and 1-2 parts of radix Glycyrrhizae. As that Chinese medicinal material are combined accord to a specific proportion, the obtained composition can obviously reduce fecal water content, regulate the symptom of spleen deficiency and dampness retention, and simultaneously promote urination of rats with spleen deficiency and dampness retention andreduce the occurrence of edema. It can also promote the absorption of serum total protein and albumin, regulate the secretion of gastric acid, promote the growth of gastric mucosa and parietal cells,remove dampness and transport spleen, promote qi and stomach to regulate the function of gastrointestinal tract.