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84 results about "Pharmaceutic industry" patented technology
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Preparation method of total flavone of Hovenia dulcisThunb
The invention discloses a method for preparing total flavonoids of hovenia acerba. The method adopts one optional method of the solvent extraction method, the solvent extraction method, the macro-porous resin adsorption method, the liquid-liquid countercurrent distribution chromatography, the supercritical fluid extraction method or the column chromatography, or the free combination of the methods. The total flavonoids of hovenia acerba is prepared from any one part of hovenia acerba, such as fruit stems, fruits, seeds, seed-cases, roots, stems, leaves, flowers, branches, skins, skin and fruit residues, etc., and the combination thereof. The prepared total flavonoids is a combination of various flavonoid active components, wherein the total flavonoids comprises the main active components as follows: quercetin, kaempferol, (+)-dihydromyricetin, myricetin, north hovenia acerba alcohol, (+)-pyrocatechin, hovenia acerba I, hovenia acerba II, hovenia acerba III and the derivates thereof; the total percentage composition of various flavonoid components by the weight is 5% to 100%; and the preparation method is applicable to pharmaceutical industry, food industry, cosmetic industry and health-care product industry.
Owner:NORTHWEST A & F UNIV
Nanostructured Biosensor Containing Neuropathy Target Esterase Activity
InactiveUS20120160708A1High sensitivityContinuous measurementImmobilised enzymesBioreactor/fermenter combinationsNeuropathy target esteraseHomeland security
The present invention provides compositions, devices and methods for detecting esterase activity. The present invention also provides devices and methods of detecting esterase inhibitors, for example, organophosphates. In particular, the present invention provides a biosensor comprising Neuropathy Target Esterase (NTE) polypeptides. Further, the present invention relates to medicine, industrial chemistry, agriculture, and homeland security.
Owner:MICHIGAN STATE UNIV +1
Novel pullulanase and gene and preparation methods thereof and engineering bacteria expressing same
ActiveCN109321552AIncrease enzyme activityImprove heat resistanceBacteriaMicroorganism based processesBacillus licheniformisFood industry
The invention belongs to the technical field of genetic engineering of enzymes, and particularly relates to novel pullulanase and a gene, an amino acid sequence and preparation methods thereof. According to the technical scheme, by screening bacteria, a strain of bacillus naganoensis which can generate pullulanase is obtained, and through an error-prone PCR technology, the pullulanase gene which is obtained through screening is modified, so that a piece of modified gene of the pullulanase is obtained, and then the novel pullulanase gene is expressed in a bacillus subtillis expression system, abacillus amyloliquefaciens expression system and a bacillus licheniformis expression system for expression separately to achieve the different preparation methods of the novel pullulanase. Meanwhile,the novel pullulanase has good application in the aspects of the food industry and the medical industry.
Owner:SHANDONG LONGKETE ENZYME PREPARATION +1
Fucosyltransferase, genetically engineered bacteria thereof and application
InactiveCN106434590AEfficient manufacturingStrong substrate specificityTransferasesFermentationBiotechnologyEscherichia coli
The invention discloses fucosyltransferase, genetically engineered bacteria thereof and an application, belongs to the technical field of gene engineering, and relates to nucleic acid and amino acid sequences from Sideroxydans lithotrophicus ES-1. Codes represent novel alpha-1, 2-fucosyltransferase SlFucT, the fucosyltransferase SlFucT obtained by separation constructs an expression vector pET15b-slfuct, and the expression vector is converted into an Escherichia coli expression strain BL21 (DE3) to realize efficient expression. The invention further provides a method for generating fucosylation oligose by alpha-1, 2-fucosyltransferase, and particularly provides an application and a method of discovering the fucosylation oligose in breast milk. The application and the method have application prospects in the field of food and medical industry.
Owner:FUJIAN AGRI & FORESTRY UNIV
Screening and application of L-proline high-producing Brevundimonas sp. (JNPP-1)
The invention belongs to the technical field of biology, and particularly relates to screening and application of an L-proline high-producing Brevundimonas sp. JNPP-1. An L-proline high-producing pseudomonad JNPP-1 is screened out for the first time; and by combining morphological characteristics and physiological biochemical characteristics, the strain JNPP-1 is identified as a Brevundimonas sp. The strain is preserved at China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.3828. The strain can produce glucoses into L-proline, and the yield can be up to 32.67g / L. The L-proline produced by the strain is one of important amino acids for synthesizing human proteins, is an important material for amino acid transfusion, and has been widely used in multiple fields, such as food, medicine and other industries.
Owner:JIANGNAN UNIV
Cholesterol oxidase gene, engineering bacterium and application thereof
ActiveCN102168098AIncrease profitIncrease productivityBacteriaMicroorganism based processesDehydrogenationGene engineering
The invention provides a cholesterol oxidase gene, cholesterol oxidase coded by the cholesterol oxidase gene, an expression vector containing a gene sequence of the cholesterol oxidase gene, a gene engineering recombinant strain containing the expression vector and a method for preparing the cholesterol oxidase. The cholesterol oxidase recombinant strain provided by the invention can be used for performing 3-site hydroxyl dehydrogenation or sterol metabolism on 3-hydroxyl steroids and can also be used for degrading sterols, so that the problem of low utilization ratio of mycobacteria and rhodococcus for degrading sterols in the sterol medicine industry is solved.
Owner:EAST CHINA UNIV OF SCI & TECH
Cryophilic xylosidase/arabinofuranosidase and preparation method and application thereof
InactiveCN102051350AHigh catalytic activityBeta-D-xylosidase highFungiBacteriaCelluloseChemical industry
The invention belongs to the field of biological engineering and provides a novel bifunctional enzyme RuXyn1 with low-temperature resistance and activities of beta-D-xylosidase and alpha-L-arabinofuranosidase. The RuXyn1 has an amino acid coding sequence shown as SEQ ID No 2 and has a nucleotide coding sequence shown as SEQ ID No 1. The RuXyn1 can be prepared by a genetic engineering method or an artificial synthesis method. The RuXyn1 has functions of the arabinofuranosidase and the xylosidase, can keep higher activity in a low-temperature environment, and can be applied to the application fields of cellulose bioconversion, chemical industry, textiles, foods, bioenergy, feed additives, pharmaceutical industry and the like.
Owner:FUDAN UNIV
EGFR positron tracer, preparation method and application thereof
InactiveCN104817542AGood tracer effectStrong specificityOrganic chemistryRadioactive preparation carriersErlotinibStructural formula
The invention discloses an EGFR positron tracer, a preparation method and application thereof. The structural formula of the EGFR positron tracer is shown as the specification. The EGFR positron tracer has good tracing effect, and compared with 18F-FDG that is the most common in clinical practice at current, the tracer has very good specificity, can achieve positive identification of epidermal growth factor receptor (EGFR) high expression tumors. The preparation method is convenient, simple and fast, can realize fully automatic production, and can meet scientific research and clinical demands. In the aspect of application, the PET imaging of 18F-Erlotinib not only can be used for screening of clinical anticancer drug Erlotinib sensitive individuals, and also has very good application prospects in detection of EGFR expression level and screening of small molecule EGFR inhibitors in the pharmaceutical industry.
Owner:NANFANG HOSPITAL OF SOUTHERN MEDICAL UNIV +1
Method for extracting alkannin naphthoquinone pigment
The invention discloses a method for extracting an alkannin naphthoquinone pigment extract and series products thereof by combining subcritical 1,1,1,2-tetrafluoroethane (R134a) extraction and molecular distillation technology. The method has the characteristics of high extraction rate, low extraction temperature, short production cycle, high product quality, low production cost and the like; andthe prepared alkannin naphthoquinone pigment extract and the refined alkannin naphthoquinone derivative series products can be used for pharmaceutical industry, food industry, cosmetics industry, printing and dyeing coloring agents and the like.
Owner:XINJIANG UNIVERSITY
Difunctional enzyme with endoglucanase/xylanase and preparation method and application thereof
The invention belongs to the technical field of biological engineering, in particular to a difunctional enzyme RuCelA with endoglucanase / xylanase and a preparation method and application thereof. The RuCelA sources from uncultured microorganism of rumen of Chinese yaks, and has a certain activity on phosphocellulose and filter. The RuCelA not only can hydrolyze polysaccharide but also can hydrolyze trisaccharide to obtain monosaccharide; meanwhile, the RuCelA has synergistic effect with xylanase to synergetically degrade xylan; and the RuCelA can be utilized to realize mono enzymatic degradation on preprocessed lignocellulose. The difunctional enzyme RuCelA of the invention can be widely applied to cellulose degradation, comprising cellulose biotransformation, chemical industry, spinning, food, bioenergy, feed ingredients, pharmaceutical Industry and other aspects.
Owner:FUDAN UNIV
Esterase, encoding genes and use thereof
The invention discloses an esterase and coding gene and application thereof; the esterase disclosed by the invention is the protein of the following (a) or (b): (a) amino acid sequence thereof is classified as sequence 2 in a sequence list; (b) the amino acid sequence of the sequence 2 in the sequence list go through the substitution and / or deletion and / or addition of one or a plurality of amino acid residues and can catalyze and hydrolyze the protein of carboxylic ester, and the protein is derived from the (a). The invention also discloses a gene for encoding the esterase and the esterase of the invention is applicable to the industries of chemicals, food, biotransformation and medicine and other related industries.
Owner:INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
Biosynthetic gene cluster of Rubrolone and application of biosynthetic gene cluster
The invention provides a biosynthetic gene cluster of Rubrolone as a tropolone natural product, Streptomyces sp. CGMCC No.11535, a preparation method for preparing Rubrolone compounds 1-4 from the biosynthetic gene cluster and the Streptomyces sp., a medicinal composition taking the Rubrolone compounds as active components, and application of the biosynthetic gene cluster to pharmacy. By adopting all gene and protein information which is relevant to Rubrolone biosynthesis, a biosynthesis mechanism of the Rubrolone as the natural product is known, and materials and knowledge are provided for further genetic modification. Genes provided by the invention and a protein thereof can be used for finding compounds, genes or proteins applicable to medicine, industry or agriculture.
Owner:KUNMING INST OF BOTANY - CHINESE ACAD OF SCI
Immune library-derived nano-antibody for specifically recognizing immunoglobulin Fc fragment
The invention belongs to the field of gene engineering, and concretely relates to a single-domain heavy chain antibody for an immunoglobulin Fc fragment. The antibody has an amino acid sequence represented by SEQ ID NO:1, and can be used in the fields of immunodetection and antigen enrichment and purification. A mutant with good properties (endophilicity, specificity and stability) can be obtained through reconstructing by using a random or site directed mutagenesis technology with an amino acid sequence is used as a precursor, and is used for developing proteins or polypeptides for being further used in medicines, industries and the agriculture.
Owner:NANCHANG UNIV
Article
ActiveCN101541508ASynthetic resin layered productsThin material handlingPolymer sciencePolypropylene
The present invention provides an article comprising a biaxially oriented polypropylene composition, wherein said composition comprises a multimodal polypropylene and a polymeric nucleating agent. The articles are suitable for use in the medical and food industries.
Owner:BOREALIS TECH OY
Pharmaceutical Compositions Comprising Canagliflozin
InactiveUS20170258761A1Improve solubilityImprove bioavailabilityOrganic active ingredientsMetabolism disorderMedicinePharmaceutical industry
The present invention belongs to the field of pharmaceutical industry and relates to a dry pharmaceutical composition comprising Canagliflozin, as well as to a process for preparing the same. Such dry pharmaceutical composition is useful as a medicament, especially for the normalization of plasma glucose levels.
Owner:SANDOZ AG
Functional digestible micelle-state casein powder and preparation method thereof
ActiveCN113057250AIncrease negative chargeStable structureChemical industryProtein composition from milkBiotechnologyMicellar casein
The invention discloses functional digestible micelle-state casein powder and a preparation method thereof. The preparation method comprises the following steps: pretreating skimmed milk, preparing micelle-state casein, preparing a compound of functional molecules and the micelle-state casein, and drying the compound, so as to obtain the functional micelle-state casein powder which is relatively good in compressibility, rapid in rehydration and relatively good in solubility and digestibility. The dissolvability and digestibility of the micelle-state casein product can be remarkably improved, so that the micelle-state casein product can be more easily digested into small molecule peptide in an organism. In addition, micellar casein is taken as a carrier, so that loading, delivery and slow release of the functional micromolecules are realized, bioavailability of the functional micromolecules is improved, and health-care effects of the casein and the functional micromolecules are brought into full play. The functional micelle-state casein powder prepared by the preparation method disclosed by the invention has wide application and wide market prospect no matter being used as a health-care product or a raw and auxiliary material in food and medicine industries.
Owner:GANSU AGRI UNIV
Phospholipase and application thereof
The invention relates to phospholipase and an application thereof. Specifically, the invention provides a polypeptide selected from: (1) a polypeptide having substitution mutations at at least one ofpositions 182, 221, 274, 323, 339, 342, 369 and 500 of an amino acid sequence shown in SEQ ID NO: 2; (2) a polypeptide comprising the polypeptide shown in (1) and a polypeptide that promotes expression and purification of the polypeptide shown in (1). The invention further provides a polynucleotide sequence encoding the polypeptide, a nucleic acid construct containing the polynucleotide sequence,a host cell, and related uses. The polypeptide provided by the invention is phospholipase and can be used in oil refining, phospholipid modification, feed modifiers, food industry and medicine industry.
Owner:WILMAR SHANGHAI BIOTECH RES & DEV CENT
Food and drug industry protective boots and boots producing method and boots technical criterion
The invention discloses safety shoes for food and medicinal industries and the production method and the technical standard of the shoes. The sides and the soles of the shoes respectively contain PVC-S-1000 51.0%, 51.2%; DOP 30.6%, 26.1%; polyester plasticizer GLOBINEX-2340-S 15.3%, 19.1%; compound rare earth stabilizer 2.1%, 2.5%; and inorganic silver antibacterial agent 1.0%, 1.2%. The safety shoes are manufactured by following steps: weighting materials of the sides and the soles of the shoes according to the weight percentage, granulating by a granulator to obtain plastic particles, respectively adding the plastic particles into an injection molding machine, and molding twice to obtain the safety shoes. The safety shoes produced by the method meet a certain technical standard, have resistance to chemical corrosion, animal and vegetal oil, impact and bacteria, and obviate hazardness existing in the food and the medicinal industries.
Owner:朱国侯
Marine microbial stachybotrys longispora and fibrinolytic active compound produced by marine microbial stachybotrys longispora
The invention relates to marine microbial stachybotrys longispora FG216. The marine microbial stachybotrys longispora FG216 is separated from ocean and produces a fibrinolytic active compound FGFC1. The marine microbial stachybotrys longispora FG216 is preserved in the China center for type culture collection (CCTCC) and has an accession number of CCTCC: M2012227. The invention also discloses the fibrinolytic active compound produced by the marine microbial stachybotrys longispora FG216. The fibrinolytic active compound FGFC1 can greatly promote plasmin cellulolytic activity, has excellent druggability and has a further research and application prospect in the medicine industry.
Owner:SHANGHAI OCEAN UNIV
Compound immobilized enzyme used for preparing statins and preparation method of compound immobilized enzyme
ActiveCN105925558AImprove utilization efficiencyEasy to separateCarbon-halide lyasesOxidoreductasesHydroxybutyric acidActive enzyme
The invention belongs to the technical field of medical industry, and mainly relates to a compound immobilized enzyme used for preparing statins, as well as a preparation method and applications of the compound immobilized enzyme. The compound immobilized enzyme provided by the invention is prepared through the manner of fixing active enzyme in an immobilized enzyme carrier through the fixing manner of adsorption, covalent binding, embedding, microencapsulation or crosslinking, wherein the active enzyme is composed of ketoreductase and / or halohydrin dehalogenase, and the mass percent of the ketoreductase in the active enzyme is 0%-100%. The immobilized enzyme product is applied to the process for producing the midbody, namely, ethyl(S)-4-chloro-3-hydroxybutanoate and / or ethyl(R)-4-cyano-3-hydroxybutyate of the products including atorvastatin, rosuvastatin, pitavastatin and the like. Compared with the liquid enzyme, the compound immobilized enzyme provided by the invention has the advantages that the production cost is reduced, the labor intensity is alleviated, the utilization efficiency of enzyme is improved, the discharge of waste water, waste gas and industrial residue is reduced, and the environment is protected.
Owner:河北周酶生物科技有限公司
Nano-antibody for specifically recognizing duck hepatitis A virus 1
ActiveCN109021099ASpecific recognition abilityStrong specificityDigestive systemBiological material analysisDuck hepatitis A virusSite-directed mutagenesis
The invention discloses a nano-antibody for specifically recognizing duck hepatitis A virus 1. The amino acid sequence of the nano-antibody is represented by SEQ ID NO.1. The nano-antibody has the advantages of small molecular weight, high stability, excellent antigen binding performance and easy expression, and provides a basis for the detection of the duck hepatitis A virus 1 and the developmentof treatment medicines. A mutant with good endophilicity, specificity and stability can be obtained through reconstructing by using a random or site-directed mutagenesis technology with the nano-antibody as a precursor, and is used for developing proteins or polypeptides for being further used in medicines, industries and agriculture.
Owner:SHANDONG AGRICULTURAL UNIVERSITY
Method for preparing free-standing film by using salt solution
The invention discloses a method for preparing a free-standing film by using a salt solution. The prepared free-standing film has a controllable thickness. The method comprises the following steps: processing a substrate, and preparing the free-standing film. The prepared free-standing film is simply mechanically peeled from water to rapidly prepare the free-standing film. The method has the advantages of simple technology, high film production efficiency and environmentally-friendly process, and the free-standing film can be widely applied to fields of medicines, industries and environmentalprotection.
Owner:CHANGCHUN UNIV OF SCI & TECH
Method of obtaining conformational polymorph of sucrose
A new sucrose is obtained through the present invention. The new sucrose has a lower solubility and a lower melting point. Thus, the new sucrose has a higher stability. The new sucrose obtained through the present invention can be used as an added functionality excipient of drug in pharmaceutical industry. And the new sucrose can also be used in sugar and food industries.
Owner:NAT CENT UNIV
Decarboxylated FR-008 derivative polyketone antibiotic and use thereof
The invention relates to decarboxylation FR-008 derivation polyketone antibiotic. It uses gene engineering to delete gene fscP to gain mutant CS103 which has the product of decarboxylation FR-008 hepa-olefin macrolide polyketone antibiotic. Compared with the FR-008 / Candicidin compound, it has lower toxicity, higher anti fungi activity, potential clinical application value, can be used in medical industry by further development.
Owner:EAST CHINA UNIV OF SCI & TECH +1
Lipase and application thereof
ActiveCN108251400AGood enzymatic propertiesImprove toleranceFatty acid esterificationHydrolasesChemistryAmino acid
The invention relates to a lipase and an application thereof, and concretely provides a polypeptide. The polypeptide is selected from a polypeptide (1) having a substitution mutation in at least one of the 150th position, the 222th position, the 285th position and the 291th position of an amino acid sequence represented by SEQ ID NO:2, and a polypeptide (2) composed of the polypeptide (1) and a polypeptide for promoting the expression and purification of the polypeptide (1). The invention also provides a polynucleotide sequence for encoding the polypeptide, a nucleic acid construct containingthe polynucleotide sequence, a host cell, and relevant uses. The polypeptide is the lipase, and can be used in oil refining, oil processing, oil chemical engineering, feed improvers, the food industry, biodiesel preparation and the medicine industry.
Owner:WILMAR SHANGHAI BIOTECH RES & DEV CENT
A chiral tridentate pnn ligand and its application in asymmetric hydrogenation reactions
ActiveCN105153229BHigh electronegativityGood chiral environmentOrganic compound preparationGroup 5/15 element organic compoundsDuloxetinePhenethyl alcohol
Owner:SHENZHEN CATALYS SCI & TECH CO LTD +2
High-purity isothiocyanate compound preparation method for industrial production
The present invention provides a high-purity isothiocyanate compound preparation method for industrial production. Specifically, in the method, organic amine and CS2 are used as raw materials to prepare the thiocarbamate, and then desulfurization is carried out, and the high-purity isothiocyanate compound is obtained by using purification, post-processing and other methods. The method in the present invention is suitable for industrial production, is simple in the post-processing, has a high yield rate, and allows the product to have a high purity, and is suitable for the production of the isothiocyanate compound in the pharmaceutical industry.
Owner:JC (WUXI) CO INC
A kind of dihydromyricetin water-soluble nano-suspension agent and its preparation method and application
ActiveCN108670953BImprove solubilityEvenly dispersedOrganic active ingredientsSolution deliveryPolymer sciencePharmaceutical industry
Owner:GUANGDONG UNIV OF TECH
Light-stable modified curdlan antioxidant as well as preparation method and application thereof
ActiveCN112048028AImprove antioxidant capacityImprove photostabilityCosmetic preparationsToilet preparationsCarboxyl radicalAnti oxidant
The invention discloses a light-stable modified curdlan antioxidant as well as a preparation method and application thereof, and belongs to the technical field of biomass materials. According to the method, the problems of steric hindrance and water solubility of physical characteristics-reaction of carboxymethyl curdlan are considered, and the modified product is prepared by adopting EDC / DMAP esterification reaction. In the modification process of the method, carboxyl of carboxymethyl curdlan is reserved to a great extent, EGCG succinylation is carried out firstly, and then reaction with remaining hydroxyl of the carboxymethyl curdlan is carried out, so that the EGCG modified curdlan antioxidant Na-CMCUD-EGCG is prepared. According to the method disclosed by the invention, the grafting rate of the EGCG is favorably improved, the grafting rate reaches 70-150mg EGCG / g Na-CMCUD-EGCG, and the water solubility of the product is also favorably improved; and the obtained product has relatively strong antioxidant activity and light stability, and can be applied to the fields of food industry, medicine industry and daily use chemicals.
Owner:JIANGNAN UNIV
Oxygen nitrogen heterocyclic heptane derivative containing exocyclic double bond and preparation method thereof
The invention provides an oxygen nitrogen heterocyclic heptane derivative containing an exocyclic double bond and a preparation method thereof. The preparation method comprises the following steps that tetraacetylene compounds are mixed with methylbenzene toluene to perform heating reaction with 2,4,4-trimethyl-2-oxazoline, and after the reaction is finished, the mixture is naturally cooled to room temperature, and a product is purified and separated to obtain the oxygen nitrogen heterocyclic heptane derivative containing the exocyclic double bond. Compared with the prior art, the invention provides the novel preparation method of the oxygen nitrogen heterocyclic heptane derivative containing the exocyclic double bond. Compared with common heterocyclic derivatives, the oxygen nitrogen heterocyclic heptane derivative containing the exocyclic double bond has the existence of the exocyclic double bond and oxygen-nitrogen heteroatoms, and a rare seven-membered ring structure is obtained, and the structure is more complex and novel. The substance of the oxygen nitrogen heterocyclic heptane derivative containing the exocyclic double bond may further have a wide range of functions in thepharmaceutical industry, and the preparation method is simple, convenient and efficient, has short reaction time and high efficiency.
Owner:ANHUI NORMAL UNIV
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