76 results about "Pharmaceutic industry" patented technology

The invention belongs to the technical field of biology, and particularly relates to screening and application of an L-proline high-producing Brevundimonas sp. JNPP-1. An L-proline high-producing pseudomonad JNPP-1 is screened out for the first time; and by combining morphological characteristics and physiological biochemical characteristics, the strain JNPP-1 is identified as a Brevundimonas sp. The strain is preserved at China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.3828. The strain can produce glucoses into L-proline, and the yield can be up to 32.67g / L. The L-proline produced by the strain is one of important amino acids for synthesizing human proteins, is an important material for amino acid transfusion, and has been widely used in multiple fields, such as food, medicine and other industries.

The invention provides a cholesterol oxidase gene, cholesterol oxidase coded by the cholesterol oxidase gene, an expression vector containing a gene sequence of the cholesterol oxidase gene, a gene engineering recombinant strain containing the expression vector and a method for preparing the cholesterol oxidase. The cholesterol oxidase recombinant strain provided by the invention can be used for performing 3-site hydroxyl dehydrogenation or sterol metabolism on 3-hydroxyl steroids and can also be used for degrading sterols, so that the problem of low utilization ratio of mycobacteria and rhodococcus for degrading sterols in the sterol medicine industry is solved.

The invention discloses an esterase and coding gene and application thereof; the esterase disclosed by the invention is the protein of the following (a) or (b): (a) amino acid sequence thereof is classified as sequence 2 in a sequence list; (b) the amino acid sequence of the sequence 2 in the sequence list go through the substitution and / or deletion and / or addition of one or a plurality of amino acid residues and can catalyze and hydrolyze the protein of carboxylic ester, and the protein is derived from the (a). The invention also discloses a gene for encoding the esterase and the esterase of the invention is applicable to the industries of chemicals, food, biotransformation and medicine and other related industries.

The invention discloses functional digestible micelle-state casein powder and a preparation method thereof. The preparation method comprises the following steps: pretreating skimmed milk, preparing micelle-state casein, preparing a compound of functional molecules and the micelle-state casein, and drying the compound, so as to obtain the functional micelle-state casein powder which is relatively good in compressibility, rapid in rehydration and relatively good in solubility and digestibility. The dissolvability and digestibility of the micelle-state casein product can be remarkably improved, so that the micelle-state casein product can be more easily digested into small molecule peptide in an organism. In addition, micellar casein is taken as a carrier, so that loading, delivery and slow release of the functional micromolecules are realized, bioavailability of the functional micromolecules is improved, and health-care effects of the casein and the functional micromolecules are brought into full play. The functional micelle-state casein powder prepared by the preparation method disclosed by the invention has wide application and wide market prospect no matter being used as a health-care product or a raw and auxiliary material in food and medicine industries.

The invention relates to phospholipase and an application thereof. Specifically, the invention provides a polypeptide selected from: (1) a polypeptide having substitution mutations at at least one ofpositions 182, 221, 274, 323, 339, 342, 369 and 500 of an amino acid sequence shown in SEQ ID NO: 2; (2) a polypeptide comprising the polypeptide shown in (1) and a polypeptide that promotes expression and purification of the polypeptide shown in (1). The invention further provides a polynucleotide sequence encoding the polypeptide, a nucleic acid construct containing the polynucleotide sequence,a host cell, and related uses. The polypeptide provided by the invention is phospholipase and can be used in oil refining, phospholipid modification, feed modifiers, food industry and medicine industry.

The invention discloses a nano-antibody for specifically recognizing duck hepatitis A virus 1. The amino acid sequence of the nano-antibody is represented by SEQ ID NO.1. The nano-antibody has the advantages of small molecular weight, high stability, excellent antigen binding performance and easy expression, and provides a basis for the detection of the duck hepatitis A virus 1 and the developmentof treatment medicines. A mutant with good endophilicity, specificity and stability can be obtained through reconstructing by using a random or site-directed mutagenesis technology with the nano-antibody as a precursor, and is used for developing proteins or polypeptides for being further used in medicines, industries and agriculture.

The invention discloses a class of chiral tridentate nitrogen phosphine ligand and its application in asymmetric hydrogenation and similar reactions. The tridentate nitrogen phosphine ligand disclosed in the present invention is the first chiral oxazoline-containing tridentate nitrogen phosphine ligand, and has been successfully applied to the hydrogenation reaction of ketones and imide salts and similar reactions with high efficiency and high selectivity . Compared with other ligands, the synthetic route is simple, the yield is high, and it is more environmentally friendly. In addition, the metal complexes of this type of ligand not only show better selectivity in asymmetric hydrogenation reactions, but also show higher conversion numbers . The iridium complex of the chiral tridentate nitrogen phosphine ligand of the present invention has successfully asymmetrically reduced β-ketone lipids to β-alcohol lipids (raw materials for the synthesis of molecular drugs duloxetine and atomoxetine), and α ‑Hydroxyacetophenone asymmetrically hydrogenates α‑hydroxyphenylethanol, and asymmetrically hydrogenates acetophenone to phenylethyl alcohol, which is of great significance for the production of the pharmaceutical industry.

The invention belongs to the technical field of preparations, and in particular relates to a dihydromyricetin water-soluble nano-suspension agent and its preparation method and application. The invention provides a preparation method of dihydromyricetin water-soluble nano-suspension, comprising the following steps: a) mixing the oil phase dissolved in dihydromyricetin and the aqueous solution dissolved in emulsifier uniformly and emulsified, and carrying out high-pressure homogeneous to obtain O / W type nanoemulsion; b) drying the O / W type nanoemulsion to obtain dihydromyricetin nanoparticles; c) mixing the dihydromyricetin nanoparticles with water to disperse , to obtain dihydromyricetin water-soluble nano-suspension. The preparation method of the present invention obtains O / W type nano-emulsion through high-pressure homogenization, and then dries to obtain dihydromyricetin nanoparticles, and dihydromyricetin nanoparticles are mixed with water to prepare dihydromyricetin water-soluble nano-suspension agent, which improves the Improve the solubility of dihydromyricetin in water, expand the application of dihydromyricetin in food and pharmaceutical industry.

The invention relates to the food and medical industries, medical cosmetics, dermatology, agriculture and the mixed feed industry. According to the invention vicinal dithioglycole (common formula RCH(SH)CH(SH)R−1 (I)) is used as a food additive, a food product, physiologically-active substances and active ingredients of forage additives and of forage, in cosmetic and / or dermatological and skin-therapeutic remedies. The invention comprises methods for producing such additives, products and remedies. The substance of formula (I) stimulates physiological processes, increases human and animal immunity, inhibits undesirable process in organisms and food products, produces curative and preventive action of skin, hair and nails and after vicinal dithioglycole is administered the intoxication effect of alcohol consumption known as hang-over is completely remored.

The invention provides a cholesterol oxidase gene, cholesterol oxidase coded by the cholesterol oxidase gene, an expression vector containing a gene sequence of the cholesterol oxidase gene, a gene engineering recombinant strain containing the expression vector and a method for preparing the cholesterol oxidase. The cholesterol oxidase recombinant strain provided by the invention can be used for performing 3-site hydroxyl dehydrogenation or sterol metabolism on 3-hydroxyl steroids and can also be used for degrading sterols, so that the problem of low utilization ratio of mycobacteria and rhodococcus for degrading sterols in the sterol medicine industry is solved.