22 results about "Reactive nitrogen species" patented technology

The invention discloses a brain-targeted active oxygen responsive hydrogen sulfide donor liposome, a preparation method and an application, and belongs to the technical field of pharmaceutical preparations, the structure of the brain-targeted active oxygen responsive hydrogen sulfide donor liposome comprises a lipid material and a hydrogen sulfide donor encapsulated in the lipid material, the lipid material comprises phospholipid, cholesterol, active oxygen sensitive lipid and brain-targeted lipid, and the lipid material comprises phospholipid, cholesterol, active oxygen sensitive lipid and brain-targeted lipid. The active oxygen sensitive lipid comprises a phospholipid lipophilic part, a polyethylene glycol hydrophilic part and an active oxygen sensitive bond, and the brain-targeted lipid comprises a phospholipid lipophilic part, a polyethylene glycol hydrophilic part and brain-targeted peptide. The hydrogen sulfide donor lipidosome can penetrate through a blood brain barrier, target neuronal cells, respond to an active oxygen environment of a focus part and sensitively release a hydrogen sulfide donor, so that hydrogen sulfide is distributed in the focus neuronal cells, active oxygen and active nitrogen substances are effectively removed, oxidation and nitration stress are inhibited, and damage and death of the neuronal cells are reduced; the growth and functional recovery of neuronal cells are promoted, and the application prospect in the aspect of treating central nervous system injury diseases is good.

The invention relates to a diatomic iron-iron site nano-enzyme constructed by relieving oxidative stress and cartilage degeneration and used for targeted osteoarthritis treatment. According to the invention, a nitrogen-doped porous carbon anchored diatomic iron nano enzyme catalyst (Fe2-NCs) with Fe-Fe dimer coordination is developed by using a'subject-object 'strategy. The Fe2-NCs protect cartilage cells from oxidative stress induced apoptosis by modulating ROS and active nitrogen species (RNS) and promoting O2 release. In addition, Fe2-NCs restores mitochondrial function by inhibiting NOX4 expression, improving ATP production, and normalizing COXIV levels. In an in-vivo OA model, the Fe2-NCs can reduce the expression of a pro-inflammatory medium COX-2 through an NF-kappa B signal channel, inhibit the up-regulation of MMP-13 and delay the degradation of type II collagen. The invention provides a new theoretical framework and methodological approach for the treatment of osteoarthritis, and has important clinical and scientific significance.

The present disclosure relates to a cosmetic composition having anti-inflammatory activity. According to the present disclosure, provided is a cosmetic composition capable of inhibiting the production of an active nitrogen substance, which is an inflammatory mediator involved in the induction of inflammation in a cell.

Novel compositions of recombinant human CC10 protein have been generated by chemically modifying the pure protein in vitro. Several new synthetic preparations containing isoforms of chemically modified rhCC10 have been generated by processes that utilize reactive oxygen species and reactive nitrogen species. These preparations contain novel isoforms of rhCC10 which have been characterized with enhanced or altered biological properties compared to the unmodified protein. Preparations containing novel isoforms may be used as standards to identify and characterize naturally occurring isoforms of native CC10 protein from blood or urine and ultimately to measure new CC10-based biomarkers to assess patient disease status. These preparations may also be used to treat respiratory, autoimmune, inflammatory, and other medical conditions that are not effectively treated with the unmodified protein.

The invention disclosed relates to a mobile and modular nitrogen production system utilizing a non-thermal plasma reactor. The system is designed for scalable on-site production of reactive molecules including Reactive Nitrogen Species (RNS) and Reactive Oxygen Species (ROS), applicable in various industries including agriculture and healthcare. Key components include one or more improved modular plasma reactors integrated within a mobile nitrogen production system. The integrated system features a plurality of improved and removable reactors, which further comprise fixed pintle injectors for fluid introduction and flow regulation, a voltage conditioner, and a ground discharge system for electrical stabilization. A controller is integrated to manage the plasma generation process, utilizing real-time data from sensors to optimize performance. The invention offers enhanced efficiency, safety, and adaptability, with applications including producing fertilizers and other reactive species-based products.

A device for creating reactive oxygen and reactive nitrogen species comprising: a plasma generator comprising at least one electrode positioned within an open-ended chamber and a centrifugal mixing chamber in fluid communication with the plasma generator, said centrifugal mixing chamber comprising a mixing chamber side wall and a blade, said blade oriented to allow passage of air in a circular motion around a central post of said centrifugal mixing chamber, and an exit nozzle on an opposing end of said centrifugal mixing chamber.

The invention discloses a preparation method of a nano-enzyme preparation as well as a product and application of the nano-enzyme preparation. The synthesis method of the nano-enzyme preparation comprises the following steps: mixing a transmembrane protein solution and a solution containing one or more nano-enzymes to prepare the nano-enzyme preparation. The nano-enzyme can target a disease site, and on one hand, the nano-enzyme removes active oxygen and active nitrogen species on the disease site; on the other hand, the nano-enzyme can be combined with cytokines of disease parts, the promotion effect of the cytokines on diseases is inhibited, and the effect of preventing and treating colitis and colitis-related colon cancer diseases is achieved. The nano-enzyme developed by the invention not only can relieve oxidative stress of diseases, but also can reduce the pro-inflammatory and tumor-promoting effects of cytokines, and can effectively treat colitis-related colon cancer caused by deterioration of colitis.

The invention discloses an anti-aging ganoderma lucidum polysaccharide-milk composition and a preparation method and application thereof.The preparation method includes the steps that ganoderma lucidum sporocarp serves as a raw material, after superfine pretreatment is conducted, ganoderma lucidum polysaccharide is extracted in a free radical mediation mode, the polysaccharide yield is effectively increased, and the activity of the polysaccharide is effectively improved; and then active oxygen free radicals (. OH) and active nitrogen free radicals (DPPH) are respectively used as indexes to preferably select the ganoderma lucidum polysaccharide-milk composition with the optimal synergistic interaction ratio, so that the antioxidant activity of the ganoderma lucidum polysaccharide is improved. The ganoderma lucidum polysaccharide-milk composition preferably selected by the method disclosed by the invention not only has the nutritional values of polysaccharide and milk, but also synergistically enhances free radical scavenging, oxidative stress resistance and aging resistance, so that high-quality utilization of ganoderma lucidum and milk is realized.

One aspect relates to a gold nanozyme containing glycol chitosan and gold particles, and a pharmaceutical composition containing the same for preventing or treating inflammatory bowel disease. It has been confirmed that the gold nanozyme according to one aspect and a composition containing the same suppress the expression of inflammatory factors, inhibit the production of intracellular reactive oxygen species (ROS) and reactive nitrogen species (RNS), and reduce the production of intracellular nitric oxide (NO). It has also been confirmed that when the gold nanozyme further contains glycyrrhizin, it inhibits the secretion of HMGB1 (High mobility group box 1). It has also been confirmed that the gold nanozyme and a composition containing the same restore the length and weight of damaged colon, making it suitable for use in the industry / market for the prevention and / or treatment of inflammatory bowel disease.

A device for ascertaining health of a tissue is disclosed. The device has a material comprising fluorescing dots (Fdots) disposed therein. The Fdots are adapted to: initiate emission of light having an emission wavelength when light having an excitation wavelength in the optical spectrum is incident thereon in a presence of nitric oxide (NO); or terminate emission of light having the emission wavelength when light having the excitation wavelength in the optical spectrum is incident thereon in a presence of nitric oxide (NO) or a reactive nitrogen species (RNS), or a reactive oxygen species (ROS); or initiate emission of light having the emission wavelength in a presence of NO, or an reactive nitrogen species, or an ROS.

One aspect according to the present invention relates to: a gold nanozyme including glycol chitosan and gold particles; and a pharmaceutical composition for preventing or treating inflammatory bowel disease, the composition comprising the gold nanozyme. The gold nanozyme and the pharmaceutical composition comprising same according to one aspect of the present invention was found to inhibit the expression of inflammatory factors, inhibit the generation of reactive oxygen species (ROS) and reactive nitrogen species (RNS) inside cells, and reduce the production of nitric oxide (NO) inside cells. In addition, the gold nanozyme was found to inhibit the secretion of Hight Mobility Group Box 1 (HMGB1) when further including glycyrrhizin. In addition, the gold nanozyme and the composition comprising same were found to enable the recovery of the length, weight, etc., of damaged intestines, and thus can be used in the inflammatory bowel disease prevention and / or treatment industry / market.

This invention discloses a solution for inhibiting keloids and its preparation method. Physiological saline is treated with low-temperature atmospheric pressure plasma (CAP) to become activated saline. The activated saline contains a large amount of reactive oxygen species and reactive nitrogen species. After injection into the body, its active ingredients induce disulfide death in fibroblasts derived from keloids, thereby inhibiting keloid formation. Compared with existing methods for inhibiting keloids, this application has several unique advantages. First, the effect and degree of CAP activation on fibroblast life and death are dose-dependent, allowing for precise control of the effect. Second, the full spectrum of active species generated by CAP can synergistically act on multiple biological targets, reducing the risk of recurrence. Third, CAP is administered through the process of generating an activated solution from a liquid medium, enabling non-invasive or minimally invasive drug delivery and greatly reducing the risk of systemic toxicity. Finally, CAP technology avoids complex biological agent preparation processes, making it simpler to operate and lower in cost. This invention fills the gap in research on low-temperature atmospheric pressure plasma inhibition of keloids.

Provided is a carbon antioxidant enzyme mimic, wherein a carbonyl group or an amine group, as a first functional group, and a hydroxyl group, as a second functional group, are bonded on a surface of the carbon antioxidant enzyme mimic. The carbon nanoparticle-based antioxidant enzyme mimic can effectively scavenge reactive oxygen species and reactive nitrogen species without metal ions. In addition, the carbon antioxidant enzyme mimic has an anti-inflammatory effect by reducing inflammatory cytokines and increasing the expression of natural antioxidant enzymes in cells.

This invention belongs to the field of nanobiomedicine technology, specifically relating to a mitochondrial-targeting copper-based nanoparticle (Qu@Cu-SS31) and its application in the preparation of drugs for treating myocardial infarction. The nanoparticles consist of quercetin (Que) and copper ions (Cu²⁺). + The nanoparticles, consisting of a self-assembly of the mitochondrial-targeting peptide SS31, exhibit a uniform, near-spherical morphology with an average particle size of 17.2 ± 1.6 nm. These nanoparticles specifically target cardiomyocyte mitochondria, responsively releasing Cu²⁺. + Furthermore, Qu@Cu-SS31 restores cardiomyocyte copper homeostasis by downregulating the copper metabolism negative regulator COMMD1, the copper uptake protein CTR1, and the copper chaperone protein CCS. Based on this, Qu@Cu-SS31 exhibits remarkable SOD-like and CAT-like enzyme activities, efficiently scavenging various reactive oxygen species / reactive nitrogen species and significantly reducing oxidative stress. Simultaneously, it inhibits NLRP3 inflammasome activation and Caspase-1-mediated pyroptosis, regulates the Bcl-2 / Bax balance to inhibit apoptosis, and promotes vascular endothelial growth factor expression and angiogenesis.

A method and system are provided for synthesizing nitrogen-containing compounds, including ammonia, urea, amino acids, and ammonium salts, under ambient temperature and pressure conditions without the use of external catalysts or high-energy input. A nitrogen-containing gas and water are introduced into an aqueous solution through a submerged bubble-diffusing component, generating microbubbles that collapse and rupture to form high-energy microenvironments. These environments facilitate in situ molecular dissociation and the formation of reactive nitrogen species, including ammonia as an intermediate. The method supports reaction with co-solutes such as carbon dioxide, organic acids, or inorganic anions to yield target compounds. Optional low-energy augmentations—such as ultraviolet irradiation, ultrasonic agitation, or shear mixing—may enhance yield and selectivity. The system is compatible with isotopic gas variants and can be configured for batch or continuous-flow operation. Applications include decentralized production of fertilizers and biochemical precursors in agricultural, hydroponic, or laboratory settings.

Compositions and methods for treating oxidative stress, and conditions where oxidative stress contributes to conditions such as diabetes, in a subject by administering an effective amount of cobinamide. Oxidative stress can be caused by, for example, increased levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS) in the patient. Compositions and methods for treating oxidative stress that contributes to diabetes-associated conditions such as cardiac fibrosis, and to aortic aneurysm, lipid or protein oxidation, or DNA damage, in a subject by administering an effective amount of cobinamide.

A liquid delivery system and a gas-liquid mixing device are provided. The gas-liquid mixing device includes a ROS (reactive oxygen species) generation module, a RNS (reactive nitrogen species) generation module, a venturi, and a control module. The ROS generation module and the RNS generation module can react with air through electrical breakdown effect to respectively generate a plurality of ROS gas particles and a plurality of RNS gas particles. The venturi can generate a negative pressure to draw in the ROS gas particles and the RNS gas particles, so as to be mixed into a liquid. The control module can control one of the ROS generation module and the RNS generation module to perform electrical breakdown effect to obtain a surge duration, and another one of the ROS generation module and the RNS generation module to perform electrical breakdown effect after the surge duration.

This application provides a CA-crosslinked, Isatis indigotica-derived carbon dots-loaded dual-responsive OPU hydrogel, its preparation method, and its applications. The hydrogel is prepared using oxidized pullulan as a backbone and chlorogenic acid as a crosslinking agent; carbon dots derived from Isatis indigotica are loaded into the hydrogel. This hydrogel exhibits excellent injectability and self-healing ability, effectively conforming to irregular wound surfaces. Under the stimulation of the acidic and oxidative microenvironment unique to infected wounds, the hydrogel gradually dissociates, achieving controlled release of Isatis indigotica carbon dots and chlorogenic acid. The Isatis indigotica carbon dots exhibit potent antibacterial activity, while chlorogenic acid synergistically works to efficiently scavenge excess reactive oxygen species and reactive nitrogen species, promoting tissue regeneration and reducing inflammatory responses. In vitro and in vivo experiments have confirmed that this hydrogel possesses excellent biocompatibility, exerting anti-infective, antioxidant, and anti-inflammatory effects in the early stages of healing, and promoting angiogenesis and accelerating wound healing in the later stages.

The invention belongs to the technical field of anti-oxidation systems, and discloses a bionic high-biological-activity anti-oxidation defense lipidosome system, application and a construction method.The bionic high-biological-activity anti-oxidation defense lipidosome system is characterized in that bionic means is adopted, and elements of an anti-oxidation defense system in biological cells, such as simulated antioxidant enzymes, water-soluble antioxidant factors and fat-soluble antioxidant factors, are combined into a whole in a bionic manner; layered entrapment is carried out in the form of liposome vesicles, and the center of the system is a water-soluble antioxidant factor; the outer side of the water-soluble antioxidant factor is coated with a lipidosome double-layer structure, and the lipidosome double-layer structure is embedded with the fat-soluble antioxidant factor; a combination of chitosan and Pt nanoclusters is arranged on the outer side of the liposome double-layer structure; the system is applied to preparation of smearing type medicines for resisting oxidative stress inflammation or medicines for improving systemic oxidative stress inflammation diseases. The system constructed by the invention has synergistic oxidation resistance, can effectively remove various active oxygen and active nitrogen, can be used for protecting cell components from oxidative damage, and can promote wound healing.

The present invention discloses a mass spectrometry imaging probe for detecting peroxynitrite, and its preparation method and application. The present invention provides a novel structural compound, the semicyanine structure of which has a positive center and can be used as a mass spectrometry detection label to capture signals; the tetrazine structure of the compound reacts with peroxynitrite to generate an oxadiazole structure, which can also be detected by mass spectrometry, and the response abundance is significantly higher than the response after reaction with other common reactive oxygen and reactive nitrogen species, showing excellent peroxynitrite selectivity, and can be used as a mass spectrometry probe for detecting peroxynitrite levels in biological samples. The probe can evaluate the production of peroxynitrite in in vivo samples by detecting the signal intensity of the corresponding product from the mass spectrometry channel, and quickly, simply and accurately analyze the spatial distribution of peroxynitrite in biological samples (such as liver tissue with ischemia-reperfusion injury) with the help of mass spectrometry imaging technology. The prior art has not disclosed the compound of the present invention, its preparation method and application.

The present invention relates to human albumin solutions and in particular to methods of preparation and uses thereof. The invention also relates to a human albumin solution having an increased mercaptoalbumin content and / or an increased transport capacity for reactive oxygen species, reactive nitrogen species and endogenous and exogenous ligands. Some embodiments of the invention have been particularly developed for the treatment of disease and will be described hereinafter with reference to the present application. However, it is understood that the present invention is not limited to this particular field of application.

This invention provides a magnesium alloy, its preparation method, and its applications. The magnesium alloy comprises a magnesium alloy body and a peroxide coating covering the surface of the magnesium alloy body. A protein carrier is adsorbed on the surface of the peroxide coating, and a NO donor is loaded onto the surface of the protein carrier. The magnesium alloy body can generate hydrogen gas in situ within the acidic tumor microenvironment, downregulating the expression of heat shock proteins in tumor cells, making tumor cells sensitive to temperature, and killing tumor cells under a low-intensity alternating magnetic field. Simultaneously, the peroxide coating stimulates the release of NO from the NO donor in the acidic tumor microenvironment, while the magnesium alloy body promotes the production of heat-activated oxygen substances inside cancer cells, which further react with NO to produce more toxic reactive nitrogen substances, achieving further killing of tumor cells.