30 results about "TMPRSS2" patented technology

Provided herein are methods and compositions for detection of a nucleic acid target in a sample. The methods and compositions use primer directed amplification in conjunction with nucleic acid fragmentation. The methods have high sensitivity even in the presence of a large amount of non-target nucleic acid. Also provided are oligonucleotides and kits useful in the method. Exemplary nucleic acid targets are those with mutant gene sequence such as mutant sequence of the EGFR, APC, TMPRSS2, ERG and ETV1 genes.

The present invention provides methods for treating or preventing influenza virus infection. The methods of the present invention comprise administering to a subject in need thereof a pharmaceutical composition comprising a type II transmembrane serine protease (TTSP) inhibitor. The TTSP inhibitor preferably functions by inhibiting the proteolytic cleavage of influenza hemagglutinin (HA0) into the functional subunits HA1 and HA2. In certain embodiments, the TTSP inhibitor is an inhibitor of transmembrane protease serine S1 member 2 (TMPRSS2), such as an anti-TMPRSS2 antibody or antigen-binding fragment thereof.

The present invention provides methods for treating or preventing influenza virus infection. The methods of the present invention comprise administering to a subject in need thereof a pharmaceutical composition comprising a type II transmembrane serine protease (TTSP) inhibitor. The TTSP inhibitor preferably functions by inhibiting the proteolytic cleavage of influenza hemagglutinin (HA0) into the functional subunits HA1 and HA2. In certain embodiments, the TTSP inhibitor is an inhibitor of transmembrane protease serine S1 member 2 (TMPRSS2), such as an anti-TMPRSS2 antibody or antigen-binding fragment thereof.

The invention relates to the technical field of gene detection and provided a method for extracting total RNA from urine of a subject and detecting TMPRSS2-ERG mRNA and PSA mRNA as well as primers which specifically amplify the TMPRSS2-ERG mRNA and PSA mRNA, wherein the primers comprise two groups of PCR amplification nucleic acid primers for detecting the TMPRSS2-ERG mRNA and PSA mRNA. The lengths of the primers are 17-24bp, and the primers specifically amplify a TMPRSS2-ERG mRNA fragment and a PAS mRNA fragment. The ratio of the TMPRSS2-ERG mRNA / PAS mRNA is measured and a TMPRSS2-ERG score is calculated by virtue of an amplification result, so that the purpose of early diagnosis of prostatic cancer and estimation of prognosis is realized.

Provided herein are methods and compositions for detection of a nucleic acid target in a sample. The methods and compositions use primer directed amplification in conjunction with nucleic acid fragmentation. The methods have high sensitivity even in the presence of a large amount of non-target nucleic acid. Also provided are oligonucleotides and kits useful in the method. Exemplary nucleic acid targets are those with mutant gene sequence such as mutant sequence of the EGFR, APC, TMPRSS2, ERG and ETV1 genes.

The invention discloses a gene chip for predicting RNA virus infected severe case. The gene chip comprises probes for capturing the following genes of ACE, ANGPT2, ASRD, CD55, FAAH, GLDC, IFITM3, IL1A, IL1B, IL1RN, IL6, IL10, IL17, IL28B, IRAK3, LGALS1, LRRC16A, MBL-2, NAMPT, NFE2L2, PI3, POPDC3, PPFIA1, SP-A2, SP-B, ST3GAL1, TLR1, TLR3, TMPRSS2, TNFA, VEGF, XKR3, ACYP2, BCL2L1, CTC1, CXCR4, NFY1,OBFC1, PXR, RTEL1, TERC, TERT, ZNF208, ZNF311, and ZNF676.

Monoclonal antibodies, or antigen-binding fragments thereof, that bind to ERG, and more specifically, to an epitope formed by amino acids 42-66 of ERG3 are disclosed. The monoclonal antibodies can be non-human antibodies (e.g., rabbit or mouse) or humanized monoclonal antibodies having the CDR regions derived from those non-human antibodies. In other embodiments, the monoclonal antibodies are chimeric, having the light and heavy chain variable regions of a non-human ERG anti-body. Methods of using the antibodies to detect ERG, or fusion proteins comprising all or part of an ERG polypeptide, such as an ERG polypeptide encoded by a TMPRSS2 / ERG, SLC45A3 / ERG, or NDRG1 / ERG fusion transcript, are also provided, including methods of detecting ERG or ERG fusion events in a clinical setting. The antibodies can also be used to inhibit the activity of ERG or fusion proteins comprising all or part of an ERG polypeptide, such as an ERG polypeptide encoded by a TMPRSS2 / ERG, SLC45A3 / ERG, or NDRG1 / ERG fusion transcript and to treat malignancies associated with overexpression of ERG or an ERG fusion event, such as prostate cancer, Ewing's sarcoma, acute myeloid leukemia, acute T-lymphoblastic leukemia, endothelial cancer, and colon cancer.

Described herein are methods, compositions and kits directed to the detection the 5′ portion of TMPRSS2 mRNA for the detection and diagnosis of prostate disease including prostate cancer and benign prostatic hyperplasia.

This invention provides a composition comprising (a) a first monoclonal antibody that (i) specifically binds to the extracellular portion of human angiotensin converting enzyme 2 (hACE2), (ii) specifically inhibits binding of SARS-CoV-2 to the extracellular portion of hACE2, and (iii) does not significantly inhibit the ability of hACE2 to cleave angiotensin II and / or a synthetic MCA-based peptide; and (b) a second monoclonal antibody that (i) specifically binds to the extracellular portion of human TMPRSS2 (hTMPRSS2), and (ii) specifically inhibits the entry into hACE2+ / hTMPRSS2+ human cells of a pseudovirus bearing SARS-CoV-2 S protein. This invention also provides related recombinant AAV vectors, recombinant AAV particles, compositions, prophylactic and therapeutic methods, and kits.

The present disclosure provides antibodies and antigen-binding fragments thereof that bind specifically to TMPRSS2 and methods of using such antibodies and fragments for treating or preventing viral infections (e.g., coronavirus infections or influenza virus infections).

The invention belongs to the field of airway inflammation medicine application, and relates to application of melatonin in preparation of a medicine for inhibiting expression of a novel coronavirus SARS-CoV-2 receptor. In the application, the melatonin can effectively alleviate OVA-induced allergic airway inflammation, and can significantly inhibit expression increase of a plurality of SARS-CoV-2 related key receptors, including ACE2, exfoliated sACE2, TMPRSS2, Calmodulin and ADAM17, in mouse and human primary bronchial epithelial cells caused by respiratory allergen exposure, so that the melatonin can be used for inhibiting the expression increase of a plurality of SARS-CoV-2 related key receptors, such as ACE2, exfoliated sACE2, TMPRSS2, Calmodulin and ADAM17. It is indicated that airway inflammatory diseases such as asthma may be a risk factor of SARS-CoV-2 infection, and the melatonin significantly inhibits expression of an SARS-CoV-2 receptor, especially ACE2 and shedding of an extracellular domain, so that a basis is provided for application of the melatonin in preparation of drugs for treating novel coronavirus pneumonia (COVID-19).

The invention belongs to the technical field of medicines, and particularly relates to application of a thioimidazolidone medicine or pharmaceutically acceptable salt thereof to preparation of a medicine for treating ACE2 and TMPRSS2 protein disorder related diseases, in particular to application to preparation of a medicine for treating COVID-19 diseases.

The invention relates to a method of post-surgical risk stratification of a prostate cancer subject, comprising determining a transmembrane protease, serine 2-ETS-related gene (TMPRSS2-ERG) fusion status in a biological sample obtained from the subject, determining a gene expression profile for each of one or more phosphodiesterase 4D variants in a biological sample obtained from the subject, determining an expression based risk score for the subject based on the gene expression profile for a selected phosphodiesterase 4D variant, and determining a post-surgical prognostic risk score for the subject based on the expression based risk score and post-surgical clinical variables of the subject, wherein the selected phosphodiesterase 4D variant is selected depending on the TMPRSS2-ERG fusion status. This may allow for an improved stratification of the subject in a post-surgical setting that may result in better post-surgical, secondary treatment decisions.

The present invention includes an antibody or antigen-binding fragment thereof that binds specifically to TMPRSS2 and methods of using such antibodies and fragments for treating or preventing viral infections (e.g., influenza virus infections).

The invention relates to a method of post-surgical risk stratification of a prostate cancer subject, comprising determining a transmembrane protease, serine 2-ETS-related gene (TMPRSS2-ERG) fusion status in a biological sample obtained from the subject, determining a gene expression profile for each of one or more phosphodiesterase 4D variants in a biological sample obtained from the subject, determining an expression based risk score for the subject based on the gene expression profile for a selected phosphodiesterase 4D variant, and determining a post-surgical prognostic risk score for the subject based on the expression based risk score and post-surgical clinical variables of the subject, wherein the selected phosphodiesterase 4D variant is selected depending on the TM-PRSS2-ERG fusion status. This may allow for an improved stratification of the subject in a post-surgical setting that may result in better post-surgical, secondary treatment decisions.

The present invention relates to methods for treating or preventing a coronavirus infection with serine protease inhibitors targeted against the host protease, transmembrane serine protease 2 (TMPRSS2), and related compositions and methods.

The invention provides a primer probe combination for early diagnosis, metastasis early warning and prognosis evaluation of prostate cancer, a kit and application of the primer probe combination and the kit. The primer probe combination comprises a first group of primer pairs, a second group of primer pairs and a third group of primer pairs, wherein the first group of primer pairs is used for detecting the PCA3 gene and has nucleotide sequences as shown in SEQ ID NO: 1-2; the second group of primer pairs is used for detecting the PSA gene and has nucleotide sequences as shown in SEQ ID NO: 4-5; the third group of primer pairs is used for detecting the TMPRSS2-ERG fusion gene and has nucleotide sequences as shown in SEQ ID NO: 7-8; the probes correspond to the three groups of primer pairs. The method not only realizes early diagnosis of the prostate cancer, but also realizes effective evaluation of metastasis and deterioration risks of the prostate cancer of a diagnosed patient, and is suitable for large-scale population screening and general survey.