The invention belongs to the technical field of cell culture and in particular relates to a method for culturing human umbilical cord mesenchymal stem cells. Aiming at the problems that conventional separated human umbilical cord mesenchymal stem cells have residual bovine serum, bovine-derived and swine-derived viruses, and the like, the invention provides the method for culturing the human umbilical cord mesenchymal stem cells. The method comprises the following steps: a, carrying out primary culture on human umbilical cord tissue with a serum-free medium till a P0 generation; b, carrying out subculture on cells of the P0 generation till a P2 generation in a culture dish; c, carrying out amplification culture on cells of the P2 generation till a fifth generation in a spinner bottle, thereby obtaining human umbilical cord mesenchymal stem cells after multiplication culture. By adopting the method provided by the invention, a serum-free culture mode is carried out in the whole process,artificially prepared cell factors and chemotactic factors are added, and the culture medium is low in sensitization and small in potential hazard factor; due to adoption of amplification culture inthe spinner bottle, the method has the advantages of rapid multiplication, short cycle, low cost, considerable multiplication cell number, and the like, thereby having wide clinical application prospects.